AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0825760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825760 MtrunA17_Chr5g0447711 76.849 311 23 3 1 262 1 311 9.44e-167 463
Msa0825760 MtrunA17_Chr5g0447701 45.570 316 101 8 1 245 1 316 1.40e-76 237
Msa0825760 MtrunA17_Chr4g0009881 74.576 59 15 0 75 133 46 104 3.79e-28 103
Msa0825760 MtrunA17_Chr4g0004911 30.412 194 105 3 75 245 214 400 3.42e-22 95.1
Msa0825760 MtrunA17_Chr4g0004911 45.763 59 32 0 182 240 185 243 6.86e-11 62.0
Msa0825760 MtrunA17_Chr7g0243891 30.189 212 115 5 74 255 245 453 4.83e-22 95.1
Msa0825760 MtrunA17_Chr3g0101131 28.384 229 121 4 62 248 255 482 1.32e-20 90.9
Msa0825760 MtrunA17_Chr3g0101131 41.791 67 36 2 175 240 237 301 1.02e-11 64.7
Msa0825760 MtrunA17_Chr5g0445311 28.780 205 114 3 74 248 229 431 2.30e-20 90.1
Msa0825760 MtrunA17_Chr5g0445311 35.000 100 62 2 142 240 161 258 1.41e-11 64.3
Msa0825760 MtrunA17_Chr5g0432161 27.556 225 120 3 68 250 267 490 1.65e-19 87.8
Msa0825760 MtrunA17_Chr3g0079691 43.902 82 46 0 166 247 480 561 3.46e-19 86.7
Msa0825760 MtrunA17_Chr3g0106491 39.806 103 61 1 149 250 332 434 4.22e-19 86.3
Msa0825760 MtrunA17_Chr7g0249301 46.667 75 40 0 169 243 280 354 5.04e-19 85.5
Msa0825760 MtrunA17_Chr7g0249311 42.222 90 52 0 166 255 377 466 5.72e-19 86.3
Msa0825760 MtrunA17_Chr5g0418191 40.230 87 52 0 166 252 305 391 1.20e-18 84.7
Msa0825760 MtrunA17_Chr5g0418191 36.957 92 55 2 171 260 139 229 1.06e-11 64.3
Msa0825760 MtrunA17_Chr4g0042831 36.559 93 59 0 169 261 266 358 2.29e-18 83.6
Msa0825760 MtrunA17_Chr7g0218081 39.080 87 53 0 166 252 395 481 2.87e-18 84.0
Msa0825760 MtrunA17_Chr7g0218081 43.077 65 34 2 177 240 224 286 2.87e-11 63.2
Msa0825760 MtrunA17_Chr7g0263531 45.333 75 41 0 166 240 346 420 1.22e-16 79.0
Msa0825760 MtrunA17_Chr7g0263531 44.118 68 37 1 173 240 181 247 5.69e-12 65.1
Msa0825760 MtrunA17_Chr1g0150801 46.377 69 37 0 169 237 90 158 1.42e-15 72.4
Msa0825760 MtrunA17_Chr8g0383251 41.667 72 42 0 169 240 96 167 1.10e-14 70.5
Msa0825760 MtrunA17_Chr4g0074001 45.833 72 39 0 169 240 347 418 1.11e-14 73.6
Msa0825760 MtrunA17_Chr4g0051881 46.575 73 39 0 169 241 166 238 2.29e-14 71.6
Msa0825760 MtrunA17_Chr8g0386241 41.176 68 40 0 170 237 92 159 2.58e-14 69.3
Msa0825760 MtrunA17_Chr1g0188941 36.111 72 46 0 170 241 40 111 3.50e-14 67.4
Msa0825760 MtrunA17_Chr2g0294231 42.105 76 44 0 166 241 188 263 9.58e-14 70.1
Msa0825760 MtrunA17_Chr1g0194481 43.421 76 43 0 166 241 143 218 1.27e-13 69.7
Msa0825760 MtrunA17_Chr8g0335811 41.892 74 43 0 166 239 139 212 1.31e-13 68.2
Msa0825760 MtrunA17_Chr7g0224801 42.647 68 39 0 170 237 82 149 1.91e-13 66.6
Msa0825760 MtrunA17_Chr7g0270191 42.667 75 43 0 166 240 130 204 2.65e-13 68.6
Msa0825760 MtrunA17_Chr5g0418781 41.758 91 48 2 169 255 112 201 2.97e-13 68.2
Msa0825760 MtrunA17_Chr3g0129321 40.278 72 43 0 169 240 106 177 3.20e-13 67.0
Msa0825760 MtrunA17_Chr5g0443131 44.156 77 42 1 169 244 145 221 6.86e-13 67.4
Msa0825760 MtrunA17_Chr3g0129361 40.541 74 44 0 166 239 129 202 2.48e-12 64.7
Msa0825760 MtrunA17_Chr4g0001361 35.870 92 50 3 168 250 149 240 2.55e-12 65.9
Msa0825760 MtrunA17_Chr2g0333851 34.118 85 55 1 158 241 146 230 2.71e-12 65.5
Msa0825760 MtrunA17_Chr3g0136961 41.667 72 39 2 169 237 124 195 4.67e-12 64.7
Msa0825760 MtrunA17_Chr1g0202461 37.234 94 53 2 175 262 255 348 5.64e-12 65.1
Msa0825760 MtrunA17_Chr4g0025061 30.928 97 66 1 147 242 326 422 1.14e-11 64.7
Msa0825760 MtrunA17_Chr5g0432071 39.130 69 41 1 181 249 104 171 1.48e-11 62.4
Msa0825760 MtrunA17_Chr7g0270831 35.443 79 49 2 171 247 206 284 1.53e-11 63.9
Msa0825760 MtrunA17_Chr1g0150281 45.070 71 37 2 169 237 127 197 1.75e-11 63.2
Msa0825760 MtrunA17_Chr4g0068541 38.889 72 44 0 169 240 196 267 1.92e-11 63.5
Msa0825760 MtrunA17_Chr3g0099641 33.696 92 58 3 171 260 241 331 2.08e-11 63.5
Msa0825760 MtrunA17_Chr2g0288871 31.915 94 63 1 148 240 272 365 2.69e-11 63.2
Msa0825760 MtrunA17_Chr8g0383351 43.548 62 35 0 178 239 157 218 2.76e-11 62.0
Msa0825760 MtrunA17_Chr7g0248721 37.975 79 48 1 159 237 119 196 3.36e-11 61.2
Msa0825760 MtrunA17_Chr4g0075201 33.708 89 58 1 153 240 239 327 7.42e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0825760 AT2G04880.2 29.461 241 129 3 62 261 123 363 5.40e-27 108
Msa0825760 AT2G04880.2 44.118 68 37 1 178 245 108 174 1.31e-12 67.4
Msa0825760 AT2G04880.1 27.820 266 125 6 62 261 123 387 2.43e-24 101
Msa0825760 AT2G04880.1 44.118 68 37 1 178 245 108 174 1.28e-12 67.8
Msa0825760 AT2G30250.1 30.412 194 107 4 75 241 196 388 9.87e-20 87.8
Msa0825760 AT5G56270.1 46.154 78 42 0 166 243 472 549 1.39e-19 88.6
Msa0825760 AT3G01080.1 29.688 192 115 3 63 240 180 365 1.83e-19 87.4
Msa0825760 AT3G01080.2 29.688 192 115 3 63 240 218 403 1.89e-19 87.8
Msa0825760 AT2G03340.1 40.449 89 53 0 166 254 400 488 2.00e-18 84.7
Msa0825760 AT2G03340.1 41.667 84 48 1 172 255 241 323 3.91e-15 75.1
Msa0825760 AT5G07100.2 44.872 78 43 0 166 243 126 203 3.71e-18 80.9
Msa0825760 AT5G07100.2 45.455 66 36 0 175 240 18 83 1.92e-13 68.2
Msa0825760 AT5G07100.4 44.872 78 43 0 166 243 126 203 3.71e-18 80.9
Msa0825760 AT5G07100.4 45.455 66 36 0 175 240 18 83 1.92e-13 68.2
Msa0825760 AT5G07100.5 44.872 78 43 0 166 243 126 203 3.71e-18 80.9
Msa0825760 AT5G07100.5 45.455 66 36 0 175 240 18 83 1.92e-13 68.2
Msa0825760 AT5G07100.3 44.872 78 43 0 166 243 126 203 3.71e-18 80.9
Msa0825760 AT5G07100.3 45.455 66 36 0 175 240 18 83 1.92e-13 68.2
Msa0825760 AT1G13960.2 44.000 75 42 0 166 240 241 315 3.80e-18 83.2
Msa0825760 AT1G13960.2 42.353 85 46 2 168 250 63 146 6.93e-15 73.9
Msa0825760 AT1G13960.1 44.000 75 42 0 166 240 394 468 3.85e-18 84.0
Msa0825760 AT1G13960.1 42.353 85 46 2 168 250 216 299 5.59e-15 74.7
Msa0825760 AT5G07100.1 44.872 78 43 0 166 243 219 296 7.86e-18 81.6
Msa0825760 AT5G07100.1 45.455 66 36 0 175 240 111 176 3.70e-13 68.6
Msa0825760 AT2G38470.1 44.304 79 44 0 166 244 347 425 9.98e-18 82.8
Msa0825760 AT4G26640.7 44.872 78 43 0 166 243 326 403 4.77e-17 80.9
Msa0825760 AT4G26640.7 37.647 85 49 3 179 262 169 250 6.45e-11 62.8
Msa0825760 AT4G26640.5 44.872 78 43 0 166 243 190 267 5.14e-17 80.1
Msa0825760 AT4G26640.5 37.647 85 49 3 179 262 33 114 8.25e-11 62.0
Msa0825760 AT4G26640.6 44.872 78 43 0 166 243 190 267 5.14e-17 80.1
Msa0825760 AT4G26640.6 37.647 85 49 3 179 262 33 114 8.25e-11 62.0
Msa0825760 AT4G26640.4 44.872 78 43 0 166 243 190 267 5.14e-17 80.1
Msa0825760 AT4G26640.4 37.647 85 49 3 179 262 33 114 8.25e-11 62.0
Msa0825760 AT4G26640.3 44.872 78 43 0 166 243 190 267 5.14e-17 80.1
Msa0825760 AT4G26640.3 37.647 85 49 3 179 262 33 114 8.25e-11 62.0
Msa0825760 AT4G26640.2 44.872 78 43 0 166 243 366 443 5.23e-17 80.9
Msa0825760 AT4G26640.2 37.647 85 49 3 179 262 209 290 6.86e-11 62.4
Msa0825760 AT4G26640.1 44.872 78 43 0 166 243 294 371 5.43e-17 80.5
Msa0825760 AT4G26640.1 37.647 85 49 3 179 262 137 218 6.79e-11 62.4
Msa0825760 AT4G26440.1 38.614 101 59 1 147 247 341 438 5.60e-17 80.5
Msa0825760 AT5G13080.1 44.118 68 38 0 170 237 56 123 6.78e-15 70.5
Msa0825760 AT5G64810.1 40.845 71 42 0 169 239 98 168 8.38e-15 71.2
Msa0825760 AT4G30935.1 38.043 92 57 0 149 240 299 390 9.19e-15 73.9
Msa0825760 AT5G26170.1 36.000 100 63 1 139 237 70 169 1.23e-14 70.5
Msa0825760 AT1G55600.1 43.836 73 41 0 168 240 294 366 4.23e-14 72.0
Msa0825760 AT5G49520.1 45.000 80 43 1 166 244 206 285 7.41e-14 71.2
Msa0825760 AT2G21900.1 38.961 77 45 1 167 241 93 169 1.09e-13 68.6
Msa0825760 AT2G47260.1 42.667 75 43 0 166 240 159 233 1.76e-13 69.7
Msa0825760 AT2G44745.1 41.892 74 43 0 166 239 130 203 3.19e-13 67.4
Msa0825760 AT1G69310.4 44.286 70 39 0 169 238 135 204 4.07e-13 68.2
Msa0825760 AT1G69310.2 44.286 70 39 0 169 238 135 204 4.07e-13 68.2
Msa0825760 AT1G69310.1 44.286 70 39 0 169 238 135 204 4.07e-13 68.2
Msa0825760 AT1G29860.1 41.975 81 46 1 166 245 121 201 7.61e-13 67.4
Msa0825760 AT4G18170.1 44.737 76 42 0 166 241 157 232 9.15e-13 67.4
Msa0825760 AT4G12020.11 41.096 73 42 1 168 240 455 526 1.21e-12 68.2
Msa0825760 AT4G12020.5 41.096 73 42 1 168 240 388 459 1.30e-12 68.2
Msa0825760 AT4G12020.7 41.096 73 42 1 168 240 455 526 1.33e-12 68.2
Msa0825760 AT4G12020.1 41.096 73 42 1 168 240 455 526 1.33e-12 68.2
Msa0825760 AT4G12020.9 41.096 73 42 1 168 240 455 526 1.33e-12 68.2
Msa0825760 AT4G23550.1 41.333 75 43 1 170 243 129 203 1.35e-12 67.0
Msa0825760 AT4G12020.3 41.096 73 42 1 168 240 455 526 1.41e-12 67.8
Msa0825760 AT4G12020.10 41.096 73 42 1 168 240 455 526 1.43e-12 67.8
Msa0825760 AT4G12020.6 41.096 73 42 1 168 240 455 526 1.48e-12 67.8
Msa0825760 AT4G12020.8 41.096 73 42 1 168 240 455 526 1.48e-12 67.8
Msa0825760 AT4G12020.4 41.096 73 42 1 168 240 455 526 1.49e-12 67.8
Msa0825760 AT4G12020.2 41.096 73 42 1 168 240 455 526 1.49e-12 67.8
Msa0825760 AT3G62340.1 47.541 61 32 0 180 240 117 177 1.52e-12 66.2
Msa0825760 AT5G41570.1 42.029 69 40 0 169 237 86 154 1.53e-12 64.7
Msa0825760 AT5G46350.1 48.438 64 33 0 180 243 182 245 1.80e-12 66.6
Msa0825760 AT1G64000.1 42.029 69 40 0 169 237 102 170 1.95e-12 64.7
Msa0825760 AT3G01970.1 41.176 68 40 0 170 237 54 121 3.19e-12 63.2
Msa0825760 AT4G39410.1 41.892 74 43 0 166 239 208 281 4.13e-12 65.5
Msa0825760 AT2G37260.1 39.706 68 41 0 170 237 338 405 5.96e-12 65.5
Msa0825760 AT2G37260.3 39.706 68 41 0 170 237 258 325 6.49e-12 65.1
Msa0825760 AT2G37260.2 39.706 68 41 0 170 237 256 323 6.50e-12 65.1
Msa0825760 AT2G25000.2 35.632 87 49 2 168 253 90 170 8.27e-12 63.5
Msa0825760 AT2G25000.4 35.632 87 49 2 168 253 90 170 8.27e-12 63.5
Msa0825760 AT2G46130.1 39.130 69 42 0 169 237 18 86 9.52e-12 60.8
Msa0825760 AT2G25000.3 35.632 87 49 2 168 253 112 192 1.12e-11 63.5
Msa0825760 AT4G01720.1 42.254 71 40 1 171 240 229 299 1.32e-11 64.7
Msa0825760 AT2G25000.1 35.632 87 49 2 168 253 133 213 1.37e-11 63.5
Msa0825760 AT4G31800.2 36.000 75 47 1 167 240 97 171 7.37e-11 61.2
Msa0825760 AT1G80840.1 39.437 71 42 1 172 241 137 207 8.00e-11 61.6

Find 49 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAATAATGCGTATTAA+TGG 0.253581 5_3:+65813239 Msa0825760:CDS
TAATGTAACAACTACCTATA+AGG 0.261776 5_3:+65813157 Msa0825760:CDS
ATGATCATGTTGTCCCTTAT+AGG 0.293854 5_3:-65813171 None:intergenic
ACTTCCACACCGGTCAAATA+TGG 0.326583 5_3:-65812670 None:intergenic
AATTCATGTGAAGGAGTATT+AGG 0.352642 5_3:-65812252 None:intergenic
TGTTCTTCCTGTTGTTGAAC+AGG 0.360275 5_3:+65812636 Msa0825760:CDS
ACAATGCTTGATTGAGGATT+AGG 0.367320 5_3:-65812598 None:intergenic
GTTACATTAGTCACATTTAG+AGG 0.369899 5_3:-65813143 None:intergenic
ACAATGTACAATATATTAAG+AGG 0.384416 5_3:-65812747 None:intergenic
ACTAGAGTTGGCGTGTTAAC+TGG 0.387351 5_3:+65812798 Msa0825760:CDS
AAATTTCATTGGTCAAATGA+TGG 0.419177 5_3:+65812535 Msa0825760:CDS
ACAGGAAGAACATGATCAAT+TGG 0.434811 5_3:-65812625 None:intergenic
TACTTTCGATGCGCCTATCC+AGG 0.436737 5_3:+65813089 Msa0825760:CDS
AATGTAACAACTACCTATAA+GGG 0.449073 5_3:+65813158 Msa0825760:CDS
TTGTAAATGATGCGTATCGC+TGG 0.453288 5_3:+65812862 Msa0825760:CDS
GCGTATCGCTGGCGCAAATA+CGG 0.465731 5_3:+65812873 Msa0825760:CDS
ATTAGGATGATTATGCTGAT+TGG 0.483420 5_3:-65812581 None:intergenic
TGTGGTCCCTGTTCAACAAC+AGG 0.490907 5_3:-65812643 None:intergenic
TTGACCGGTGTGGAAGTTCA+AGG 0.496310 5_3:+65812676 Msa0825760:CDS
AATTTCATTGGTCAAATGAT+GGG 0.502281 5_3:+65812536 Msa0825760:CDS
GTTCTTCCTGTTGTTGAACA+GGG 0.504635 5_3:+65812637 Msa0825760:CDS
TTTCATTGTGCATCCTGGAT+AGG 0.505091 5_3:-65813102 None:intergenic
AATGCTGATGCTACTAGAGT+TGG 0.527944 5_3:+65812786 Msa0825760:CDS
AGAAACTAATGATGTAGCTA+TGG 0.532105 5_3:+65812188 Msa0825760:CDS
TTACATTAGTCACATTTAGA+GGG 0.537229 5_3:-65813142 None:intergenic
TCATGAACCACCGACTGGTA+GGG 0.554218 5_3:+65813190 Msa0825760:CDS
CCACATCAACCATATTTGAC+CGG 0.557825 5_3:+65812661 Msa0825760:CDS
ACTCGATGTTGTACTTCAGA+AGG 0.565037 5_3:-65812135 None:intergenic
GGTGGTACAATGCTTGATTG+AGG 0.567041 5_3:-65812604 None:intergenic
TGTAGCTATGGCTTGTGACT+TGG 0.571450 5_3:+65812200 Msa0825760:CDS
TATTGATTAGATTGTATCAG+AGG 0.571636 5_3:-65812276 None:intergenic
ATCATGAACCACCGACTGGT+AGG 0.573060 5_3:+65813189 Msa0825760:CDS
CATGAACCACCGACTGGTAG+GGG 0.577060 5_3:+65813191 Msa0825760:CDS
ATCAGAGGTAATTCATGTGA+AGG 0.594434 5_3:-65812261 None:intergenic
CCGGTCAAATATGGTTGATG+TGG 0.605641 5_3:-65812661 None:intergenic
GGAAGAACATGATCAATTGG+TGG 0.607875 5_3:-65812622 None:intergenic
TACATTAGTCACATTTAGAG+GGG 0.623510 5_3:-65813141 None:intergenic
CATGATCATGAACCACCGAC+TGG 0.628103 5_3:+65813185 Msa0825760:CDS
ACATCGAGTGAATCCTCAGA+GGG 0.633099 5_3:+65812149 Msa0825760:CDS
GACAATGTGATCTCCCTCTG+AGG 0.634875 5_3:-65812162 None:intergenic
CACATATGCAACACTCGCAG+AGG 0.656505 5_3:+65812229 Msa0825760:CDS
TATTTGTAACAGAGTCATGA+CGG 0.659620 5_3:-65813217 None:intergenic
GGTGTAACTGAAATATTCGA+CGG 0.662056 5_3:-65812560 None:intergenic
ATCACCTTGAACTTCCACAC+CGG 0.673408 5_3:-65812680 None:intergenic
CACTTGAAGTCTGAACAACA+AGG 0.680480 5_3:-65812830 None:intergenic
TCAACCATATTTGACCGGTG+TGG 0.682556 5_3:+65812666 Msa0825760:CDS
CGGACACCCCTACCAGTCGG+TGG 0.689482 5_3:-65813197 None:intergenic
TGACGGACACCCCTACCAGT+CGG 0.690312 5_3:-65813200 None:intergenic
AACATCGAGTGAATCCTCAG+AGG 0.727570 5_3:+65812148 Msa0825760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGTACAATATATTAAG+AGG - chr5_3:65812750-65812769 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG + chr5_3:65812907-65812926 Msa0825760:CDS 20.0%
!! ACAATGTACAATATATTAAG+AGG - chr5_3:65812750-65812769 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG + chr5_3:65812907-65812926 Msa0825760:CDS 20.0%
!! TATTGATTAGATTGTATCAG+AGG - chr5_3:65812279-65812298 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG + chr5_3:65812524-65812543 Msa0825760:CDS 25.0%
! AAATTTCATTGGTCAAATGA+TGG + chr5_3:65812535-65812554 Msa0825760:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG + chr5_3:65812536-65812555 Msa0825760:CDS 25.0%
! TTACATTAGTCACATTTAGA+GGG - chr5_3:65813145-65813164 None:intergenic 25.0%
! TAATGTAACAACTACCTATA+AGG + chr5_3:65813157-65813176 Msa0825760:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG + chr5_3:65813158-65813177 Msa0825760:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG + chr5_3:65813239-65813258 Msa0825760:CDS 25.0%
!! TATTGATTAGATTGTATCAG+AGG - chr5_3:65812279-65812298 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG + chr5_3:65812524-65812543 Msa0825760:CDS 25.0%
! AAATTTCATTGGTCAAATGA+TGG + chr5_3:65812535-65812554 Msa0825760:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG + chr5_3:65812536-65812555 Msa0825760:CDS 25.0%
! TTACATTAGTCACATTTAGA+GGG - chr5_3:65813145-65813164 None:intergenic 25.0%
! TAATGTAACAACTACCTATA+AGG + chr5_3:65813157-65813176 Msa0825760:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG + chr5_3:65813158-65813177 Msa0825760:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG + chr5_3:65813239-65813258 Msa0825760:CDS 25.0%
AGAAACTAATGATGTAGCTA+TGG + chr5_3:65812188-65812207 Msa0825760:CDS 30.0%
!! AATTCATGTGAAGGAGTATT+AGG - chr5_3:65812255-65812274 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG + chr5_3:65812325-65812344 Msa0825760:intron 30.0%
GGCTATAAGTGGAAAAAATA+TGG + chr5_3:65812336-65812355 Msa0825760:intron 30.0%
TGAATGTGTGCATTTGTAAT+AGG - chr5_3:65812497-65812516 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG - chr5_3:65812584-65812603 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG + chr5_3:65812891-65812910 Msa0825760:CDS 30.0%
ACGAGTTCAAAATAAAAGCT+AGG - chr5_3:65812938-65812957 None:intergenic 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG - chr5_3:65813076-65813095 None:intergenic 30.0%
TACATTAGTCACATTTAGAG+GGG - chr5_3:65813144-65813163 None:intergenic 30.0%
GTTACATTAGTCACATTTAG+AGG - chr5_3:65813146-65813165 None:intergenic 30.0%
TATTTGTAACAGAGTCATGA+CGG - chr5_3:65813220-65813239 None:intergenic 30.0%
AGAAACTAATGATGTAGCTA+TGG + chr5_3:65812188-65812207 Msa0825760:CDS 30.0%
!! AATTCATGTGAAGGAGTATT+AGG - chr5_3:65812255-65812274 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG + chr5_3:65812325-65812344 Msa0825760:intron 30.0%
GGCTATAAGTGGAAAAAATA+TGG + chr5_3:65812336-65812355 Msa0825760:intron 30.0%
TGAATGTGTGCATTTGTAAT+AGG - chr5_3:65812497-65812516 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG - chr5_3:65812584-65812603 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG + chr5_3:65812891-65812910 Msa0825760:CDS 30.0%
ACGAGTTCAAAATAAAAGCT+AGG - chr5_3:65812938-65812957 None:intergenic 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG - chr5_3:65813076-65813095 None:intergenic 30.0%
TACATTAGTCACATTTAGAG+GGG - chr5_3:65813144-65813163 None:intergenic 30.0%
GTTACATTAGTCACATTTAG+AGG - chr5_3:65813146-65813165 None:intergenic 30.0%
TATTTGTAACAGAGTCATGA+CGG - chr5_3:65813220-65813239 None:intergenic 30.0%
ATCAGAGGTAATTCATGTGA+AGG - chr5_3:65812264-65812283 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG + chr5_3:65812315-65812334 Msa0825760:intron 35.0%
GGTGTAACTGAAATATTCGA+CGG - chr5_3:65812563-65812582 None:intergenic 35.0%
ACAATGCTTGATTGAGGATT+AGG - chr5_3:65812601-65812620 None:intergenic 35.0%
ACAGGAAGAACATGATCAAT+TGG - chr5_3:65812628-65812647 None:intergenic 35.0%
! GTAATTTCTGGAAGATTGGA+GGG - chr5_3:65813072-65813091 None:intergenic 35.0%
! AGTAATTTCTGGAAGATTGG+AGG - chr5_3:65813073-65813092 None:intergenic 35.0%
!!! TACTTTTTCATTGTGCATCC+TGG - chr5_3:65813110-65813129 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG - chr5_3:65813174-65813193 None:intergenic 35.0%
ATCAGAGGTAATTCATGTGA+AGG - chr5_3:65812264-65812283 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG + chr5_3:65812315-65812334 Msa0825760:intron 35.0%
GGTGTAACTGAAATATTCGA+CGG - chr5_3:65812563-65812582 None:intergenic 35.0%
ACAATGCTTGATTGAGGATT+AGG - chr5_3:65812601-65812620 None:intergenic 35.0%
ACAGGAAGAACATGATCAAT+TGG - chr5_3:65812628-65812647 None:intergenic 35.0%
! GTAATTTCTGGAAGATTGGA+GGG - chr5_3:65813072-65813091 None:intergenic 35.0%
! AGTAATTTCTGGAAGATTGG+AGG - chr5_3:65813073-65813092 None:intergenic 35.0%
!!! TACTTTTTCATTGTGCATCC+TGG - chr5_3:65813110-65813129 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG - chr5_3:65813174-65813193 None:intergenic 35.0%
ACTCGATGTTGTACTTCAGA+AGG - chr5_3:65812138-65812157 None:intergenic 40.0%
TCGCTCAATCATACGTGAAA+TGG + chr5_3:65812302-65812321 Msa0825760:intron 40.0%
GGAAGAACATGATCAATTGG+TGG - chr5_3:65812625-65812644 None:intergenic 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG + chr5_3:65812636-65812655 Msa0825760:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG + chr5_3:65812637-65812656 Msa0825760:CDS 40.0%
CCACATCAACCATATTTGAC+CGG + chr5_3:65812661-65812680 Msa0825760:CDS 40.0%
AATGCTGATGCTACTAGAGT+TGG + chr5_3:65812786-65812805 Msa0825760:CDS 40.0%
CACTTGAAGTCTGAACAACA+AGG - chr5_3:65812833-65812852 None:intergenic 40.0%
TTGTAAATGATGCGTATCGC+TGG + chr5_3:65812862-65812881 Msa0825760:CDS 40.0%
GCGCAAATACGGAAGAAAAA+TGG + chr5_3:65812884-65812903 Msa0825760:CDS 40.0%
TTTCATTGTGCATCCTGGAT+AGG - chr5_3:65813105-65813124 None:intergenic 40.0%
ACTCGATGTTGTACTTCAGA+AGG - chr5_3:65812138-65812157 None:intergenic 40.0%
TCGCTCAATCATACGTGAAA+TGG + chr5_3:65812302-65812321 Msa0825760:intron 40.0%
GGAAGAACATGATCAATTGG+TGG - chr5_3:65812625-65812644 None:intergenic 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG + chr5_3:65812636-65812655 Msa0825760:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG + chr5_3:65812637-65812656 Msa0825760:CDS 40.0%
CCACATCAACCATATTTGAC+CGG + chr5_3:65812661-65812680 Msa0825760:CDS 40.0%
AATGCTGATGCTACTAGAGT+TGG + chr5_3:65812786-65812805 Msa0825760:CDS 40.0%
CACTTGAAGTCTGAACAACA+AGG - chr5_3:65812833-65812852 None:intergenic 40.0%
TTGTAAATGATGCGTATCGC+TGG + chr5_3:65812862-65812881 Msa0825760:CDS 40.0%
GCGCAAATACGGAAGAAAAA+TGG + chr5_3:65812884-65812903 Msa0825760:CDS 40.0%
TTTCATTGTGCATCCTGGAT+AGG - chr5_3:65813105-65813124 None:intergenic 40.0%
AACATCGAGTGAATCCTCAG+AGG + chr5_3:65812148-65812167 Msa0825760:CDS 45.0%
ACATCGAGTGAATCCTCAGA+GGG + chr5_3:65812149-65812168 Msa0825760:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG + chr5_3:65812200-65812219 Msa0825760:CDS 45.0%
GGTGGTACAATGCTTGATTG+AGG - chr5_3:65812607-65812626 None:intergenic 45.0%
! CCGGTCAAATATGGTTGATG+TGG - chr5_3:65812664-65812683 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG + chr5_3:65812666-65812685 Msa0825760:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG - chr5_3:65812673-65812692 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG - chr5_3:65812683-65812702 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG + chr5_3:65812798-65812817 Msa0825760:CDS 45.0%
GGCGCATCGAAAGTAATTTC+TGG - chr5_3:65813084-65813103 None:intergenic 45.0%
AACATCGAGTGAATCCTCAG+AGG + chr5_3:65812148-65812167 Msa0825760:CDS 45.0%
ACATCGAGTGAATCCTCAGA+GGG + chr5_3:65812149-65812168 Msa0825760:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG + chr5_3:65812200-65812219 Msa0825760:CDS 45.0%
GGTGGTACAATGCTTGATTG+AGG - chr5_3:65812607-65812626 None:intergenic 45.0%
! CCGGTCAAATATGGTTGATG+TGG - chr5_3:65812664-65812683 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG + chr5_3:65812666-65812685 Msa0825760:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG - chr5_3:65812673-65812692 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG - chr5_3:65812683-65812702 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG + chr5_3:65812798-65812817 Msa0825760:CDS 45.0%
GGCGCATCGAAAGTAATTTC+TGG - chr5_3:65813084-65813103 None:intergenic 45.0%
GACAATGTGATCTCCCTCTG+AGG - chr5_3:65812165-65812184 None:intergenic 50.0%
CACATATGCAACACTCGCAG+AGG + chr5_3:65812229-65812248 Msa0825760:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG - chr5_3:65812646-65812665 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG + chr5_3:65812676-65812695 Msa0825760:CDS 50.0%
TACTTTCGATGCGCCTATCC+AGG + chr5_3:65813089-65813108 Msa0825760:CDS 50.0%
CATGATCATGAACCACCGAC+TGG + chr5_3:65813185-65813204 Msa0825760:CDS 50.0%
! ATCATGAACCACCGACTGGT+AGG + chr5_3:65813189-65813208 Msa0825760:CDS 50.0%
! TCATGAACCACCGACTGGTA+GGG + chr5_3:65813190-65813209 Msa0825760:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG - chr5_3:65812165-65812184 None:intergenic 50.0%
CACATATGCAACACTCGCAG+AGG + chr5_3:65812229-65812248 Msa0825760:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG - chr5_3:65812646-65812665 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG + chr5_3:65812676-65812695 Msa0825760:CDS 50.0%
TACTTTCGATGCGCCTATCC+AGG + chr5_3:65813089-65813108 Msa0825760:CDS 50.0%
CATGATCATGAACCACCGAC+TGG + chr5_3:65813185-65813204 Msa0825760:CDS 50.0%
! ATCATGAACCACCGACTGGT+AGG + chr5_3:65813189-65813208 Msa0825760:CDS 50.0%
! TCATGAACCACCGACTGGTA+GGG + chr5_3:65813190-65813209 Msa0825760:CDS 50.0%
GCGTATCGCTGGCGCAAATA+CGG + chr5_3:65812873-65812892 Msa0825760:CDS 55.0%
! CATGAACCACCGACTGGTAG+GGG + chr5_3:65813191-65813210 Msa0825760:CDS 55.0%
GCGTATCGCTGGCGCAAATA+CGG + chr5_3:65812873-65812892 Msa0825760:CDS 55.0%
! CATGAACCACCGACTGGTAG+GGG + chr5_3:65813191-65813210 Msa0825760:CDS 55.0%
! TGACGGACACCCCTACCAGT+CGG - chr5_3:65813203-65813222 None:intergenic 60.0%
! TGACGGACACCCCTACCAGT+CGG - chr5_3:65813203-65813222 None:intergenic 60.0%
CGGACACCCCTACCAGTCGG+TGG - chr5_3:65813200-65813219 None:intergenic 70.0%
CGGACACCCCTACCAGTCGG+TGG - chr5_3:65813200-65813219 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 65812096 65813271 65812096 ID=Msa0825760;Name=Msa0825760
chr5_3 mRNA 65812096 65813271 65812096 ID=Msa0825760-mRNA-1;Parent=Msa0825760;Name=Msa0825760-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0|0.25|4|0|262
chr5_3 exon 65812096 65812315 65812096 ID=Msa0825760-mRNA-1:exon:20659;Parent=Msa0825760-mRNA-1
chr5_3 exon 65812518 65812697 65812518 ID=Msa0825760-mRNA-1:exon:20660;Parent=Msa0825760-mRNA-1
chr5_3 exon 65812727 65812928 65812727 ID=Msa0825760-mRNA-1:exon:20661;Parent=Msa0825760-mRNA-1
chr5_3 exon 65813085 65813271 65813085 ID=Msa0825760-mRNA-1:exon:20662;Parent=Msa0825760-mRNA-1
chr5_3 CDS 65812096 65812315 65812096 ID=Msa0825760-mRNA-1:cds;Parent=Msa0825760-mRNA-1
chr5_3 CDS 65812518 65812697 65812518 ID=Msa0825760-mRNA-1:cds;Parent=Msa0825760-mRNA-1
chr5_3 CDS 65812727 65812928 65812727 ID=Msa0825760-mRNA-1:cds;Parent=Msa0825760-mRNA-1
chr5_3 CDS 65813085 65813271 65813085 ID=Msa0825760-mRNA-1:cds;Parent=Msa0825760-mRNA-1
Gene Sequence

>Msa0825760

ATGGTTTTGTCACTGCAAAATGTTGATCACAATGATTCTCCTTCTGAAGTACAACATCGAGTGAATCCTCAGAGGGAGATCACATTGTCACGAGAAACTAATGATGTAGCTATGGCTTGTGACTTGGTATTATCACATATGCAACACTCGCAGAGGCCTAATACTCCTTCACATGAATTACCTCTGATACAATCTAATCAATACACTCGCTCAATCATACACTGTCGTGCAAAAAAGAAATTTCATTGGTCAAATGATGGGACCGTCGAATATTTCAGTTACACCAATCAGCATAATCATCCTAATCCTCAATCAAGCATTGTACCACCAATTGATCATGTTCTTCCTGTTGTTGAACAGGGACCACATCAACCATATTTGACCGGTGTGGAAGTTCAAGTTTCAATATTACATGAAAAACCTCTTAATATATTGTACATTGTTGTGCATGCAGACAACAATGCTGATGCTACTAGAGTTGGCGTGTTAACTGGTGAACTTCACCTTGTTGTTCAGACTTCAAGTGCAAATGAAGTTGTAAATGATGCGTATCGCTGGCGCAAATACGGAAGAAAAATGGTTAATGGAAAAACAATTCAAAGAAATTACTTTCGATGCGCCTATCCAGGATGCACAATGAAAAAGTATGTTGAAAAGTCCCCTCTAAATGTGACTAATGTAACAACTACCTATAAGGGACAACATGATCATGAACCACCGACTGGTAGGGGTGTCCGTCATGACTCTGTTACAAATACTCAAATAATGCGTATTAATGGCGAGCCATAA
Protein sequence

>Msa0825760

MVLSLQNVDHNDSPSEVQHRVNPQREITLSRETNDVAMACDLVLSHMQHSQRPNTPSHELPLIQSNQYTRSIIHCRAKKKFHWSNDGTVEYFSYTNQHNHPNPQSSIVPPIDHVLPVVEQGPHQPYLTGVEVQVSILHEKPLNILYIVVHADNNADATRVGVLTGELHLVVQTSSANEVVNDAYRWRKYGRKMVNGKTIQRNYFRCAYPGCTMKKYVEKSPLNVTNVTTTYKGQHDHEPPTGRGVRHDSVTNTQIMRINGEP*