AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826810 MtrunA17_Chr5g0448431 99.390 164 1 0 1 164 1 164 2.08e-121 338
Msa0826810 MtrunA17_Chr5g0395301 27.632 152 87 3 14 142 17 168 5.03e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826810 AT5G11340.1 77.439 164 37 0 1 164 1 164 8.73e-97 276
Msa0826810 AT5G16800.1 23.926 163 101 3 4 143 20 182 2.25e-12 63.2
Msa0826810 AT5G16800.2 23.926 163 101 3 4 143 20 182 3.70e-12 62.8
Msa0826810 AT5G16800.3 23.926 163 101 3 4 143 20 182 5.53e-12 62.4

Find 73 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAGCTATCTTACAATTT+TGG 0.052794 5_3:+67598327 Msa0826810:three_prime_UTR
CATTTCAGTGCTAGTTATTT+TGG 0.053551 5_3:+67598130 Msa0826810:three_prime_UTR
AAATTTCTTATAAAAGTTAA+TGG 0.199299 5_3:-67598002 None:intergenic
TTTAGGATCTATTGACTAAT+TGG 0.220792 5_3:+67598514 Msa0826810:three_prime_UTR
GCTGGTGGGAGAGGGAAGAT+AGG 0.267925 5_3:-67598311 None:intergenic
TTCAATTTCACAAGCAAAAT+CGG 0.296681 5_3:+67595320 Msa0826810:five_prime_UTR
AATTTGGTACTAGAATCTTT+TGG 0.317504 5_3:+67598367 Msa0826810:three_prime_UTR
TGATGTTTACGTAATAGTTC+TGG 0.333615 5_3:-67598048 None:intergenic
CTGAAATGTTATTTCTTTGT+TGG 0.338231 5_3:-67598115 None:intergenic
CGGGTTTACATCATGACTTT+AGG 0.340929 5_3:+67597599 Msa0826810:CDS
GGCTTCGCAGGAACTGGATT+GGG 0.348608 5_3:-67595208 None:intergenic
AGGAACTGGATTGGGCTTCT+TGG 0.356940 5_3:-67595200 None:intergenic
TGTAAGATAGCTGGTGGGAG+AGG 0.360366 5_3:-67598320 None:intergenic
TCATGATAATCAAACTGTTT+AGG 0.365120 5_3:+67598497 Msa0826810:three_prime_UTR
AGAAGGAAGGCGGGCAAGTT+CGG 0.365269 5_3:+67597579 Msa0826810:CDS
GAAGGAAGGCGGGCAAGTTC+GGG 0.367625 5_3:+67597580 Msa0826810:CDS
TGAAATGTTATTTCTTTGTT+GGG 0.378604 5_3:-67598114 None:intergenic
TTGATGGGCTTCGCAGGAAC+TGG 0.382868 5_3:-67595214 None:intergenic
TATTTGTCGTTGTAACGAAC+AGG 0.385879 5_3:-67595632 None:intergenic
TTTAAATTTAGCATTGGAAA+TGG 0.391033 5_3:-67598220 None:intergenic
CCTTACCGTGGACTTGGTAT+TGG 0.408366 5_3:+67597632 Msa0826810:CDS
TGTTATTTCTTTGTTGGGCT+AGG 0.418834 5_3:-67598109 None:intergenic
TTAGCGCCTTACCGTGGACT+TGG 0.429752 5_3:+67597626 Msa0826810:CDS
TGATAGAAAAGAACCTAGTT+TGG 0.435318 5_3:-67595687 None:intergenic
GAATAGAGAAGTAGAATCAA+TGG 0.442448 5_3:+67595523 Msa0826810:intron
GGGCTTCGCAGGAACTGGAT+TGG 0.446032 5_3:-67595209 None:intergenic
GAAGTATCAATATCATTAGA+TGG 0.453181 5_3:+67595557 Msa0826810:CDS
TTGTTGAACCAAGCTATACT+TGG 0.458797 5_3:-67598415 None:intergenic
GGGTAAAGCGGTAGCGGCTA+TGG 0.461748 5_3:-67595283 None:intergenic
CGCCTTCCTTCTTCTCAAGC+CGG 0.465600 5_3:-67597568 None:intergenic
GTAAGATAGCTGGTGGGAGA+GGG 0.473436 5_3:-67598319 None:intergenic
TGCATGCCGGCTTGAGAAGA+AGG 0.476399 5_3:+67597562 Msa0826810:CDS
CTGGTGGGAGAGGGAAGATA+GGG 0.482693 5_3:-67598310 None:intergenic
GATGAGTTGATGGGCTTCGC+AGG 0.491117 5_3:-67595220 None:intergenic
ATGAGTTGAGTTGGCTGAGT+GGG 0.492310 5_3:-67595346 None:intergenic
TCCCAAAATTGTAAGATAGC+TGG 0.496559 5_3:-67598329 None:intergenic
TGATGAATGAGATGAGTTGA+TGG 0.499292 5_3:-67595230 None:intergenic
TCCGCTGATTTCACCAAACT+AGG 0.503020 5_3:+67595674 Msa0826810:CDS
AATAGAGAAGTAGAATCAAT+GGG 0.520505 5_3:+67595524 Msa0826810:intron
TCCGGCGAGATGAGTTGAGT+TGG 0.520696 5_3:-67595355 None:intergenic
TACTACAGTGACATTTGTGT+TGG 0.528723 5_3:+67597533 Msa0826810:CDS
ACCCAACATCTCAAAACTCC+AGG 0.529812 5_3:+67595406 Msa0826810:five_prime_UTR
GGTTGAGACAAAACTCAGTT+GGG 0.533539 5_3:-67598549 None:intergenic
AAACAAGGGTAAAGCGGTAG+CGG 0.547015 5_3:-67595289 None:intergenic
CGCGAAGCAAAACATTTCTG+AGG 0.547039 5_3:+67597946 Msa0826810:CDS
GGCTTGAGAAGAAGGAAGGC+GGG 0.549023 5_3:+67597570 Msa0826810:CDS
TATAGCTTGGTTCAACAAGA+TGG 0.551909 5_3:+67598420 Msa0826810:three_prime_UTR
AATTGAAATGGTTACAAACA+AGG 0.557285 5_3:-67595304 None:intergenic
CAAAATTGTAAGATAGCTGG+TGG 0.561640 5_3:-67598326 None:intergenic
GAAGTAGAATCAATGGGAGC+TGG 0.565209 5_3:+67595530 Msa0826810:exon
TTTGGGTTTATGCAGGAACA+AGG 0.566199 5_3:+67597897 Msa0826810:intron
AATTGGGGATCTCTTTGCTA+AGG 0.566447 5_3:+67598271 Msa0826810:three_prime_UTR
GTGTTGGCGCAATTGCATGC+CGG 0.568057 5_3:+67597549 Msa0826810:CDS
GATGTTGGGTAATGTGATGA+TGG 0.569672 5_3:-67595393 None:intergenic
GCCAACTCAACTCATCTCGC+CGG 0.575000 5_3:+67595354 Msa0826810:five_prime_UTR
TGCCGGCTTGAGAAGAAGGA+AGG 0.583442 5_3:+67597566 Msa0826810:CDS
AGGTTGAGACAAAACTCAGT+TGG 0.586889 5_3:-67598550 None:intergenic
ATTGAAATGGTTACAAACAA+GGG 0.598777 5_3:-67595303 None:intergenic
CTTGTGACAACATAACAGTC+TGG 0.600627 5_3:-67598076 None:intergenic
AGCTTGGTTCAACAAGATGG+TGG 0.610806 5_3:+67598423 Msa0826810:three_prime_UTR
CCAATACCAAGTCCACGGTA+AGG 0.611714 5_3:-67597632 None:intergenic
GCTTGGTTCAACAAGATGGT+GGG 0.619201 5_3:+67598424 Msa0826810:three_prime_UTR
CGGCTTGAGAAGAAGGAAGG+CGG 0.620221 5_3:+67597569 Msa0826810:CDS
GGTTACAAACAAGGGTAAAG+CGG 0.625791 5_3:-67595295 None:intergenic
ATTTGTCGTTGTAACGAACA+GGG 0.627678 5_3:-67595631 None:intergenic
TCTTACCAATACCAAGTCCA+CGG 0.630487 5_3:-67597637 None:intergenic
TGAGTTGAGTTGGCTGAGTG+GGG 0.631926 5_3:-67595345 None:intergenic
ACCTAGTTTGGTGAAATCAG+CGG 0.645414 5_3:-67595675 None:intergenic
GATGAATGAGATGAGTTGAT+GGG 0.646306 5_3:-67595229 None:intergenic
CAGACTGTTATGTTGTCACA+AGG 0.649227 5_3:+67598077 Msa0826810:CDS
GATGAGTTGAGTTGGCTGAG+TGG 0.705617 5_3:-67595347 None:intergenic
AAAATTGTAAGATAGCTGGT+GGG 0.714409 5_3:-67598325 None:intergenic
GTGACAACATAACAGTCTGG+TGG 0.757743 5_3:-67598073 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCATATATTATAAGTTA+GGG - chr5_3:67597025-67597044 None:intergenic 10.0%
!!! ATTTTCATATATTATAAGTT+AGG - chr5_3:67597026-67597045 None:intergenic 10.0%
!! TAAATTAAAACACTTAAAAA+GGG - chr5_3:67597049-67597068 None:intergenic 10.0%
!! TTAAATTAAAACACTTAAAA+AGG - chr5_3:67597050-67597069 None:intergenic 10.0%
!! AAATTTCTTATAAAAGTTAA+TGG - chr5_3:67598005-67598024 None:intergenic 10.0%
!! ATAAAAATAATATTAGAACC+TGG - chr5_3:67595427-67595446 None:intergenic 15.0%
!! TAAAAAAAAATCCTAAACAA+AGG - chr5_3:67596012-67596031 None:intergenic 15.0%
!!! TTTTAAAATGAAAAGTTGTT+AGG + chr5_3:67596047-67596066 Msa0826810:intron 15.0%
!!! TTTTTTTTTTTTTCTTTCAG+AGG + chr5_3:67596092-67596111 Msa0826810:intron 15.0%
!! ATTATTATAACAACAGAAAT+TGG + chr5_3:67596618-67596637 Msa0826810:intron 15.0%
!! TTTCATATATTATAAGTTAG+GGG - chr5_3:67597024-67597043 None:intergenic 15.0%
!! AAGTATAATAATCAATCTTA+AGG - chr5_3:67597503-67597522 None:intergenic 15.0%
!!! ATAGTATTGAATTTTGAATT+TGG + chr5_3:67598351-67598370 Msa0826810:three_prime_UTR 15.0%
!!! AGTTTTGCTTTGTTTTTATT+TGG - chr5_3:67595262-67595281 None:intergenic 20.0%
!!! AGAATATTAACCTTCTAAAA+TGG - chr5_3:67595901-67595920 None:intergenic 20.0%
!! CAAATTGAAACAAATCATTA+TGG - chr5_3:67595963-67595982 None:intergenic 20.0%
!!! AACAGTTTTAGTGAAATTTT+AGG + chr5_3:67595983-67596002 Msa0826810:intron 20.0%
!!! ACAGTTTTAGTGAAATTTTA+GGG + chr5_3:67595984-67596003 Msa0826810:intron 20.0%
!! TTAAAACATAGTGTATTCAT+TGG + chr5_3:67596267-67596286 Msa0826810:intron 20.0%
!!! CATGTATTTTATTTCATACT+AGG + chr5_3:67596397-67596416 Msa0826810:intron 20.0%
!! GAGTTTCTATCTAATTATTT+AGG + chr5_3:67596977-67596996 Msa0826810:intron 20.0%
!! TTCATATATTATAAGTTAGG+GGG - chr5_3:67597023-67597042 None:intergenic 20.0%
!!! TTTAAGTTTTAACAAGAACA+TGG + chr5_3:67597065-67597084 Msa0826810:intron 20.0%
!! AGAAAGATTTCAAAACAAAA+AGG - chr5_3:67597201-67597220 None:intergenic 20.0%
!! ACATACAACAACATTAATAA+AGG - chr5_3:67597463-67597482 None:intergenic 20.0%
!! ATAATAATCAATCTTAAGGT+TGG - chr5_3:67597499-67597518 None:intergenic 20.0%
!! AGAAATTTGATATGAATCTT+TGG + chr5_3:67597879-67597898 Msa0826810:intron 20.0%
!! GAAATTTGATATGAATCTTT+GGG + chr5_3:67597880-67597899 Msa0826810:intron 20.0%
!!! TGAAATGTTATTTCTTTGTT+GGG - chr5_3:67598117-67598136 None:intergenic 20.0%
!!! CTATTTATTGAATTTTGCTA+TGG + chr5_3:67598177-67598196 Msa0826810:three_prime_UTR 20.0%
!!! TTTAAATTTAGCATTGGAAA+TGG - chr5_3:67598223-67598242 None:intergenic 20.0%
!!! TACTGTTTTAAATTTAGCAT+TGG - chr5_3:67598229-67598248 None:intergenic 20.0%
!!! TTTGCTTTGTTTTTATTTGG+TGG - chr5_3:67595259-67595278 None:intergenic 25.0%
! ATTGAAATGGTTACAAACAA+GGG - chr5_3:67595306-67595325 None:intergenic 25.0%
! AATTGAAATGGTTACAAACA+AGG - chr5_3:67595307-67595326 None:intergenic 25.0%
!! TTTTGCTTGTGAAATTGAAA+TGG - chr5_3:67595319-67595338 None:intergenic 25.0%
! TTCAATTTCACAAGCAAAAT+CGG + chr5_3:67595320-67595339 Msa0826810:five_prime_UTR 25.0%
! AATAGAGAAGTAGAATCAAT+GGG + chr5_3:67595524-67595543 Msa0826810:intron 25.0%
!! GAAGTATCAATATCATTAGA+TGG + chr5_3:67595557-67595576 Msa0826810:CDS 25.0%
! AAACACTGTTCAAAGATTAA+GGG - chr5_3:67596214-67596233 None:intergenic 25.0%
! AAAACACTGTTCAAAGATTA+AGG - chr5_3:67596215-67596234 None:intergenic 25.0%
!! TTCTTTTGATGCTTGATTTA+AGG + chr5_3:67597162-67597181 Msa0826810:intron 25.0%
! GATAAAAGGACATTAATCAT+AGG - chr5_3:67597225-67597244 None:intergenic 25.0%
!! TTTTATGAGATGTGGATAAA+AGG - chr5_3:67597239-67597258 None:intergenic 25.0%
!!! CTGAAAGTTTTTAACTTAGT+TGG + chr5_3:67597336-67597355 Msa0826810:intron 25.0%
!!! TTTTTAACTTAGTTGGAACT+TGG + chr5_3:67597343-67597362 Msa0826810:intron 25.0%
!! TTCAGTTTTTCATGATAACA+TGG + chr5_3:67597415-67597434 Msa0826810:intron 25.0%
!!! CTGAAATGTTATTTCTTTGT+TGG - chr5_3:67598118-67598137 None:intergenic 25.0%
!!! ATTTCAGTGCTAGTTATTTT+GGG + chr5_3:67598131-67598150 Msa0826810:three_prime_UTR 25.0%
!! AATTTGGTACTAGAATCTTT+TGG + chr5_3:67598367-67598386 Msa0826810:three_prime_UTR 25.0%
!! ATTTGGTACTAGAATCTTTT+GGG + chr5_3:67598368-67598387 Msa0826810:three_prime_UTR 25.0%
! TCATGATAATCAAACTGTTT+AGG + chr5_3:67598497-67598516 Msa0826810:three_prime_UTR 25.0%
!! TTTAGGATCTATTGACTAAT+TGG + chr5_3:67598514-67598533 Msa0826810:three_prime_UTR 25.0%
GAATAGAGAAGTAGAATCAA+TGG + chr5_3:67595523-67595542 Msa0826810:intron 30.0%
TGATAGAAAAGAACCTAGTT+TGG - chr5_3:67595690-67595709 None:intergenic 30.0%
TAGCTTAGAGTGTGAAATAA+TGG - chr5_3:67595713-67595732 None:intergenic 30.0%
AAAAATAAGCCAATCCAAAC+CGG + chr5_3:67595861-67595880 Msa0826810:intron 30.0%
!! ATTAACCTTCTAAAATGGAC+AGG - chr5_3:67595896-67595915 None:intergenic 30.0%
! ATAGTGTATTCATTGGCTAA+AGG + chr5_3:67596274-67596293 Msa0826810:intron 30.0%
AAATACATGAGAGAGAAAAC+AGG - chr5_3:67596386-67596405 None:intergenic 30.0%
TTTCATACTAGGATTAACGT+CGG + chr5_3:67596408-67596427 Msa0826810:intron 30.0%
GAAAAATTGAAGGAGGAATT+TGG - chr5_3:67596668-67596687 None:intergenic 30.0%
ATGAAGAAAAACAAACCCTT+GGG + chr5_3:67596718-67596737 Msa0826810:intron 30.0%
AAAGCATGGAGTAAAATCTA+AGG - chr5_3:67596808-67596827 None:intergenic 30.0%
AACAGAAATCGAATGCAAAA+AGG - chr5_3:67596927-67596946 None:intergenic 30.0%
! TTTTTGCATTCGATTTCTGT+TGG + chr5_3:67596926-67596945 Msa0826810:intron 30.0%
! TTTTGCATTCGATTTCTGTT+GGG + chr5_3:67596927-67596946 Msa0826810:intron 30.0%
ATATATTATAAGTTAGGGGG+AGG - chr5_3:67597020-67597039 None:intergenic 30.0%
AAGAACATGGAATACATAGA+GGG + chr5_3:67597078-67597097 Msa0826810:intron 30.0%
! TTTTGATGCTTGATTTAAGG+AGG + chr5_3:67597165-67597184 Msa0826810:intron 30.0%
GATTCCAATAAAGATGACAA+CGG - chr5_3:67597267-67597286 None:intergenic 30.0%
GTAGTGAAACTATATAGTCT+TGG - chr5_3:67597289-67597308 None:intergenic 30.0%
AAAGCACACATATATCAAAC+AGG - chr5_3:67597397-67597416 None:intergenic 30.0%
CATGATAACATGGATGATTT+AGG + chr5_3:67597425-67597444 Msa0826810:intron 30.0%
AATATGTGTTTGTGGTACAA+GGG - chr5_3:67597753-67597772 None:intergenic 30.0%
! ACATATTGGAGACTTTTGTT+AGG + chr5_3:67597766-67597785 Msa0826810:intron 30.0%
AGATAGCAAACTTAAAGAAC+AGG - chr5_3:67597849-67597868 None:intergenic 30.0%
TGATGTTTACGTAATAGTTC+TGG - chr5_3:67598051-67598070 None:intergenic 30.0%
! CATTTCAGTGCTAGTTATTT+TGG + chr5_3:67598130-67598149 Msa0826810:three_prime_UTR 30.0%
!! TTTCAGTGCTAGTTATTTTG+GGG + chr5_3:67598132-67598151 Msa0826810:three_prime_UTR 30.0%
AAAATTGTAAGATAGCTGGT+GGG - chr5_3:67598328-67598347 None:intergenic 30.0%
! ACCAGCTATCTTACAATTTT+GGG + chr5_3:67598328-67598347 Msa0826810:three_prime_UTR 30.0%
! TTTGGTACTAGAATCTTTTG+GGG + chr5_3:67598369-67598388 Msa0826810:three_prime_UTR 30.0%
!!! TTTTAGAACCAAGTATAGCT+TGG + chr5_3:67598407-67598426 Msa0826810:three_prime_UTR 30.0%
!! GATGAATGAGATGAGTTGAT+GGG - chr5_3:67595232-67595251 None:intergenic 35.0%
! TGATGAATGAGATGAGTTGA+TGG - chr5_3:67595233-67595252 None:intergenic 35.0%
!!! GCTTTGTTTTTATTTGGTGG+AGG - chr5_3:67595256-67595275 None:intergenic 35.0%
ATTATTTGTGCGCCACAAAT+CGG - chr5_3:67595483-67595502 None:intergenic 35.0%
ATTTGTCGTTGTAACGAACA+GGG - chr5_3:67595634-67595653 None:intergenic 35.0%
TATTTGTCGTTGTAACGAAC+AGG - chr5_3:67595635-67595654 None:intergenic 35.0%
TGCTTATGCAAATACAGCTT+AGG + chr5_3:67595757-67595776 Msa0826810:intron 35.0%
CTAAAATGGACAGGAACTAA+GGG - chr5_3:67595887-67595906 None:intergenic 35.0%
! TCTAAAATGGACAGGAACTA+AGG - chr5_3:67595888-67595907 None:intergenic 35.0%
! TAGTTCCTGTCCATTTTAGA+AGG + chr5_3:67595888-67595907 Msa0826810:intron 35.0%
!!! ATTTTAGGGTGCCTTTGTTT+AGG + chr5_3:67595998-67596017 Msa0826810:intron 35.0%
TGTATAGAGGTTTCATTGGA+TGG - chr5_3:67596356-67596375 None:intergenic 35.0%
TGAGAGAGAAAACAGGAAAT+CGG - chr5_3:67596379-67596398 None:intergenic 35.0%
!! TTACTTTTAGCCAAGAGTAG+TGG - chr5_3:67596466-67596485 None:intergenic 35.0%
CAGTCATAGCAAATCCTTTA+AGG + chr5_3:67596506-67596525 Msa0826810:intron 35.0%
GGAGTAGATGGAAAAAAAGA+GGG + chr5_3:67596556-67596575 Msa0826810:intron 35.0%
!! ATTTTTCAGTTCCCCCATAT+TGG + chr5_3:67596681-67596700 Msa0826810:intron 35.0%
!! TTTTTCAGTTCCCCCATATT+GGG + chr5_3:67596682-67596701 Msa0826810:intron 35.0%
ATACAAAATCCCCCCAATAT+GGG - chr5_3:67596697-67596716 None:intergenic 35.0%
CATGAAGAAAAACAAACCCT+TGG + chr5_3:67596717-67596736 Msa0826810:intron 35.0%
TGAAGAAAAACAAACCCTTG+GGG + chr5_3:67596719-67596738 Msa0826810:intron 35.0%
! CTGCTATTTGATATCTTCCA+TGG + chr5_3:67596898-67596917 Msa0826810:intron 35.0%
TTTGCATTCGATTTCTGTTG+GGG + chr5_3:67596928-67596947 Msa0826810:intron 35.0%
CAAGAACATGGAATACATAG+AGG + chr5_3:67597077-67597096 Msa0826810:intron 35.0%
GTAGATGTGTTGAATGCTAA+TGG + chr5_3:67597120-67597139 Msa0826810:intron 35.0%
TACTACAGTGACATTTGTGT+TGG + chr5_3:67597533-67597552 Msa0826810:CDS 35.0%
CAATATGTGTTTGTGGTACA+AGG - chr5_3:67597754-67597773 None:intergenic 35.0%
CTTGTACCACAAACACATAT+TGG + chr5_3:67597752-67597771 Msa0826810:intron 35.0%
AAGTCTCCAATATGTGTTTG+TGG - chr5_3:67597761-67597780 None:intergenic 35.0%
TTAGGTGCAAGCATCAAATT+AGG + chr5_3:67597784-67597803 Msa0826810:intron 35.0%
! ATGAATCTTTGGGTTTATGC+AGG + chr5_3:67597890-67597909 Msa0826810:intron 35.0%
!! TGTTATTTCTTTGTTGGGCT+AGG - chr5_3:67598112-67598131 None:intergenic 35.0%
! ATCCCCAATTGCTTCTTTTT+GGG - chr5_3:67598261-67598280 None:intergenic 35.0%
CAAAATTGTAAGATAGCTGG+TGG - chr5_3:67598329-67598348 None:intergenic 35.0%
! CACCAGCTATCTTACAATTT+TGG + chr5_3:67598327-67598346 Msa0826810:three_prime_UTR 35.0%
TCCCAAAATTGTAAGATAGC+TGG - chr5_3:67598332-67598351 None:intergenic 35.0%
TTGTTGAACCAAGCTATACT+TGG - chr5_3:67598418-67598437 None:intergenic 35.0%
TATAGCTTGGTTCAACAAGA+TGG + chr5_3:67598420-67598439 Msa0826810:three_prime_UTR 35.0%
GGTTACAAACAAGGGTAAAG+CGG - chr5_3:67595298-67595317 None:intergenic 40.0%
! TGGACACAGATTGAGTTTTC+CGG - chr5_3:67595376-67595395 None:intergenic 40.0%
GATGTTGGGTAATGTGATGA+TGG - chr5_3:67595396-67595415 None:intergenic 40.0%
!!! ACCTGGAGTTTTGAGATGTT+GGG - chr5_3:67595410-67595429 None:intergenic 40.0%
!!! AACCTGGAGTTTTGAGATGT+TGG - chr5_3:67595411-67595430 None:intergenic 40.0%
!! CGATTTTCCTTTCCGATTTG+TGG + chr5_3:67595468-67595487 Msa0826810:intron 40.0%
! ACCTAGTTTGGTGAAATCAG+CGG - chr5_3:67595678-67595697 None:intergenic 40.0%
CAGAGGTAACACATGAACTT+TGG + chr5_3:67596109-67596128 Msa0826810:intron 40.0%
GGCTAAAGGAAAATGCTTGT+TGG + chr5_3:67596288-67596307 Msa0826810:intron 40.0%
TCGGTGTATAGAGGTTTCAT+TGG - chr5_3:67596360-67596379 None:intergenic 40.0%
AACAGGAAATCGGTGTATAG+AGG - chr5_3:67596369-67596388 None:intergenic 40.0%
GTGCTATATACCACTACTCT+TGG + chr5_3:67596453-67596472 Msa0826810:intron 40.0%
!! ATTTGCTATGACTGACTCCA+GGG - chr5_3:67596500-67596519 None:intergenic 40.0%
! CTGTGTTTTGCGAGTTTAGA+GGG + chr5_3:67596529-67596548 Msa0826810:intron 40.0%
! TGTGTTTTGCGAGTTTAGAG+GGG + chr5_3:67596530-67596549 Msa0826810:intron 40.0%
GGGAGTAGATGGAAAAAAAG+AGG + chr5_3:67596555-67596574 Msa0826810:intron 40.0%
GGGAACTGAAAAATTGAAGG+AGG - chr5_3:67596675-67596694 None:intergenic 40.0%
TGGGGGAACTGAAAAATTGA+AGG - chr5_3:67596678-67596697 None:intergenic 40.0%
! TTTTCAGTTCCCCCATATTG+GGG + chr5_3:67596683-67596702 Msa0826810:intron 40.0%
TACAAAATCCCCCCAATATG+GGG - chr5_3:67596696-67596715 None:intergenic 40.0%
GATACAAAATCCCCCCAATA+TGG - chr5_3:67596698-67596717 None:intergenic 40.0%
GAAGAAAAACAAACCCTTGG+GGG + chr5_3:67596720-67596739 Msa0826810:intron 40.0%
!!! GGTTTTGGGTAAAATGAAGG+AGG - chr5_3:67596755-67596774 None:intergenic 40.0%
!!! GAGGGTTTTGGGTAAAATGA+AGG - chr5_3:67596758-67596777 None:intergenic 40.0%
TAAGGCTATGTTTGCGAGTT+TGG - chr5_3:67596790-67596809 None:intergenic 40.0%
! CATGCTTTCGTTGCACTTTT+CGG + chr5_3:67596820-67596839 Msa0826810:intron 40.0%
!! TAGCAGTTCTGAATTGCACA+GGG - chr5_3:67596884-67596903 None:intergenic 40.0%
!! ATAGCAGTTCTGAATTGCAC+AGG - chr5_3:67596885-67596904 None:intergenic 40.0%
CGAATGCAAAAAGGATACCA+TGG - chr5_3:67596918-67596937 None:intergenic 40.0%
TATTATAAGTTAGGGGGAGG+CGG - chr5_3:67597017-67597036 None:intergenic 40.0%
! GGGAAGTTCTCTCTTTTCTT+TGG + chr5_3:67597098-67597117 Msa0826810:intron 40.0%
!!! CGGACTGTTTTTATGAGATG+TGG - chr5_3:67597247-67597266 None:intergenic 40.0%
CAGTCCGTTGTCATCTTTAT+TGG + chr5_3:67597260-67597279 Msa0826810:intron 40.0%
CGGGTTTACATCATGACTTT+AGG + chr5_3:67597599-67597618 Msa0826810:CDS 40.0%
TCTTACCAATACCAAGTCCA+CGG - chr5_3:67597640-67597659 None:intergenic 40.0%
! TTTGGGTTTATGCAGGAACA+AGG + chr5_3:67597897-67597916 Msa0826810:intron 40.0%
CTTGTGACAACATAACAGTC+TGG - chr5_3:67598079-67598098 None:intergenic 40.0%
CAGACTGTTATGTTGTCACA+AGG + chr5_3:67598077-67598096 Msa0826810:CDS 40.0%
!!! GTGCTAGTTATTTTGGGGAA+AGG + chr5_3:67598137-67598156 Msa0826810:three_prime_UTR 40.0%
! TCCCCAATTGCTTCTTTTTG+GGG - chr5_3:67598260-67598279 None:intergenic 40.0%
! GATCCCCAATTGCTTCTTTT+TGG - chr5_3:67598262-67598281 None:intergenic 40.0%
AATTGGGGATCTCTTTGCTA+AGG + chr5_3:67598271-67598290 Msa0826810:three_prime_UTR 40.0%
AAACAAGGGTAAAGCGGTAG+CGG - chr5_3:67595292-67595311 None:intergenic 45.0%
!! ATGAGTTGAGTTGGCTGAGT+GGG - chr5_3:67595349-67595368 None:intergenic 45.0%
ACCCAACATCTCAAAACTCC+AGG + chr5_3:67595406-67595425 Msa0826810:five_prime_UTR 45.0%
GAAGTAGAATCAATGGGAGC+TGG + chr5_3:67595530-67595549 Msa0826810:exon 45.0%
!! TCCGCTGATTTCACCAAACT+AGG + chr5_3:67595674-67595693 Msa0826810:CDS 45.0%
TCGTTTACGTTCGCGTTTGT+CGG - chr5_3:67596322-67596341 None:intergenic 45.0%
AAGTAAACCTGCTGAAACCC+TGG + chr5_3:67596480-67596499 Msa0826810:intron 45.0%
!! GATTTGCTATGACTGACTCC+AGG - chr5_3:67596501-67596520 None:intergenic 45.0%
CTCGCAAAACACAGCCTTAA+AGG - chr5_3:67596523-67596542 None:intergenic 45.0%
! GCTGTGTTTTGCGAGTTTAG+AGG + chr5_3:67596528-67596547 Msa0826810:intron 45.0%
! TTTTGCGAGTTTAGAGGGGA+GGG + chr5_3:67596534-67596553 Msa0826810:intron 45.0%
TTTCAGTTCCCCCATATTGG+GGG + chr5_3:67596684-67596703 Msa0826810:intron 45.0%
ACAAAATCCCCCCAATATGG+GGG - chr5_3:67596695-67596714 None:intergenic 45.0%
TGGGTAAAATGAAGGAGGCA+AGG - chr5_3:67596750-67596769 None:intergenic 45.0%
CTATGTTTGCGAGTTTGGAG+GGG - chr5_3:67596785-67596804 None:intergenic 45.0%
GCTATGTTTGCGAGTTTGGA+GGG - chr5_3:67596786-67596805 None:intergenic 45.0%
! CGAAAAGTGCAACGAAAGCA+TGG - chr5_3:67596822-67596841 None:intergenic 45.0%
CGCGAAGCAAAACATTTCTG+AGG + chr5_3:67597946-67597965 Msa0826810:CDS 45.0%
GTGACAACATAACAGTCTGG+TGG - chr5_3:67598076-67598095 None:intergenic 45.0%
AGCCCCCAAAAAGAAGCAAT+TGG + chr5_3:67598254-67598273 Msa0826810:three_prime_UTR 45.0%
GCCCCCAAAAAGAAGCAATT+GGG + chr5_3:67598255-67598274 Msa0826810:three_prime_UTR 45.0%
! CCCCAATTGCTTCTTTTTGG+GGG - chr5_3:67598259-67598278 None:intergenic 45.0%
CCCCCAAAAAGAAGCAATTG+GGG + chr5_3:67598256-67598275 Msa0826810:three_prime_UTR 45.0%
AGCTTGGTTCAACAAGATGG+TGG + chr5_3:67598423-67598442 Msa0826810:three_prime_UTR 45.0%
GCTTGGTTCAACAAGATGGT+GGG + chr5_3:67598424-67598443 Msa0826810:three_prime_UTR 45.0%
!! TGAGTTGAGTTGGCTGAGTG+GGG - chr5_3:67595348-67595367 None:intergenic 50.0%
!! GATGAGTTGAGTTGGCTGAG+TGG - chr5_3:67595350-67595369 None:intergenic 50.0%
TTGTGCGCCACAAATCGGAA+AGG - chr5_3:67595478-67595497 None:intergenic 50.0%
!! AACTAAGGGCCGGTTTGGAT+TGG - chr5_3:67595873-67595892 None:intergenic 50.0%
AATGGACAGGAACTAAGGGC+CGG - chr5_3:67595883-67595902 None:intergenic 50.0%
! GTTTTGCGAGTTTAGAGGGG+AGG + chr5_3:67596533-67596552 Msa0826810:intron 50.0%
TTTGCGAGTTTAGAGGGGAG+GGG + chr5_3:67596535-67596554 Msa0826810:intron 50.0%
TTCAGTTCCCCCATATTGGG+GGG + chr5_3:67596685-67596704 Msa0826810:intron 50.0%
GGCTATGTTTGCGAGTTTGG+AGG - chr5_3:67596787-67596806 None:intergenic 50.0%
CCAATACCAAGTCCACGGTA+AGG - chr5_3:67597635-67597654 None:intergenic 50.0%
! CCTTACCGTGGACTTGGTAT+TGG + chr5_3:67597632-67597651 Msa0826810:CDS 50.0%
GTAAGATAGCTGGTGGGAGA+GGG - chr5_3:67598322-67598341 None:intergenic 50.0%
TGTAAGATAGCTGGTGGGAG+AGG - chr5_3:67598323-67598342 None:intergenic 50.0%
GGCTTCGCAGGAACTGGATT+GGG - chr5_3:67595211-67595230 None:intergenic 55.0%
!! TTGATGGGCTTCGCAGGAAC+TGG - chr5_3:67595217-67595236 None:intergenic 55.0%
!! GATGAGTTGATGGGCTTCGC+AGG - chr5_3:67595223-67595242 None:intergenic 55.0%
GCCAACTCAACTCATCTCGC+CGG + chr5_3:67595354-67595373 Msa0826810:five_prime_UTR 55.0%
! TCCGGCGAGATGAGTTGAGT+TGG - chr5_3:67595358-67595377 None:intergenic 55.0%
ACAGGAACTAAGGGCCGGTT+TGG - chr5_3:67595878-67595897 None:intergenic 55.0%
! ACTGACTCCAGGGTTTCAGC+AGG - chr5_3:67596490-67596509 None:intergenic 55.0%
TTAGAGGGGAGGGGAGTAGA+TGG + chr5_3:67596544-67596563 Msa0826810:intron 55.0%
TTTGCGAGTTTGGAGGGGAC+GGG - chr5_3:67596780-67596799 None:intergenic 55.0%
GTTTGCGAGTTTGGAGGGGA+CGG - chr5_3:67596781-67596800 None:intergenic 55.0%
! GTGTTGGCGCAATTGCATGC+CGG + chr5_3:67597549-67597568 Msa0826810:CDS 55.0%
!! TGCATGCCGGCTTGAGAAGA+AGG + chr5_3:67597562-67597581 Msa0826810:CDS 55.0%
!! TGCCGGCTTGAGAAGAAGGA+AGG + chr5_3:67597566-67597585 Msa0826810:CDS 55.0%
CGCCTTCCTTCTTCTCAAGC+CGG - chr5_3:67597571-67597590 None:intergenic 55.0%
CGGCTTGAGAAGAAGGAAGG+CGG + chr5_3:67597569-67597588 Msa0826810:CDS 55.0%
GGCTTGAGAAGAAGGAAGGC+GGG + chr5_3:67597570-67597589 Msa0826810:CDS 55.0%
AGAAGGAAGGCGGGCAAGTT+CGG + chr5_3:67597579-67597598 Msa0826810:CDS 55.0%
!!! GGTGTTTTAGCGCCTTACCG+TGG + chr5_3:67597620-67597639 Msa0826810:CDS 55.0%
TTAGCGCCTTACCGTGGACT+TGG + chr5_3:67597626-67597645 Msa0826810:CDS 55.0%
CTGGTGGGAGAGGGAAGATA+GGG - chr5_3:67598313-67598332 None:intergenic 55.0%
GGGCTTCGCAGGAACTGGAT+TGG - chr5_3:67595212-67595231 None:intergenic 60.0%
! GGGTAAAGCGGTAGCGGCTA+TGG - chr5_3:67595286-67595305 None:intergenic 60.0%
GAGGCAAGGTAGTCCCCCAA+GGG - chr5_3:67596736-67596755 None:intergenic 60.0%
GAAGGAAGGCGGGCAAGTTC+GGG + chr5_3:67597580-67597599 Msa0826810:CDS 60.0%
GCTGGTGGGAGAGGGAAGAT+AGG - chr5_3:67598314-67598333 None:intergenic 60.0%
GGAGGCAAGGTAGTCCCCCA+AGG - chr5_3:67596737-67596756 None:intergenic 65.0%
! GGAGGGGACGGGAGGGTTTT+GGG - chr5_3:67596769-67596788 None:intergenic 65.0%
! TGGAGGGGACGGGAGGGTTT+TGG - chr5_3:67596770-67596789 None:intergenic 65.0%
CGAGTTTGGAGGGGACGGGA+GGG - chr5_3:67596776-67596795 None:intergenic 65.0%
GCGAGTTTGGAGGGGACGGG+AGG - chr5_3:67596777-67596796 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 67595202 67598564 67595202 ID=Msa0826810;Name=Msa0826810
chr5_3 mRNA 67595202 67598564 67595202 ID=Msa0826810-mRNA-1;Parent=Msa0826810;Name=Msa0826810-mRNA-1;_AED=0.04;_eAED=0.04;_QI=239|1|1|1|0.66|0.5|4|435|164
chr5_3 exon 67595202 67595427 67595202 ID=Msa0826810-mRNA-1:exon:21161;Parent=Msa0826810-mRNA-1
chr5_3 exon 67595529 67595695 67595529 ID=Msa0826810-mRNA-1:exon:21162;Parent=Msa0826810-mRNA-1
chr5_3 exon 67597531 67597653 67597531 ID=Msa0826810-mRNA-1:exon:21163;Parent=Msa0826810-mRNA-1
chr5_3 exon 67597912 67598564 67597912 ID=Msa0826810-mRNA-1:exon:21164;Parent=Msa0826810-mRNA-1
chr5_3 five_prime_UTR 67595202 67595427 67595202 ID=Msa0826810-mRNA-1:five_prime_utr;Parent=Msa0826810-mRNA-1
chr5_3 five_prime_UTR 67595529 67595541 67595529 ID=Msa0826810-mRNA-1:five_prime_utr;Parent=Msa0826810-mRNA-1
chr5_3 CDS 67595542 67595695 67595542 ID=Msa0826810-mRNA-1:cds;Parent=Msa0826810-mRNA-1
chr5_3 CDS 67597531 67597653 67597531 ID=Msa0826810-mRNA-1:cds;Parent=Msa0826810-mRNA-1
chr5_3 CDS 67597912 67598129 67597912 ID=Msa0826810-mRNA-1:cds;Parent=Msa0826810-mRNA-1
chr5_3 three_prime_UTR 67598130 67598564 67598130 ID=Msa0826810-mRNA-1:three_prime_utr;Parent=Msa0826810-mRNA-1
Gene Sequence

>Msa0826810

ATGGGAGCTGGTCGTGAAGTATCAATATCATTAGATGGAGTAAGAGACAAGAACATTATGCAACTCAAGAAACTTAATCTTGCTCTTTTCCCTGTTCGTTACAACGACAAATACTATGCTGATGCTCTTGCTTCCGCTGATTTCACCAAACTAGCTTACTACAGTGACATTTGTGTTGGCGCAATTGCATGCCGGCTTGAGAAGAAGGAAGGCGGGCAAGTTCGGGTTTACATCATGACTTTAGGTGTTTTAGCGCCTTACCGTGGACTTGGTATTGGAACAAGGCTGCTGAATCATGTTATTGATCTTTGCGCGAAGCAAAACATTTCTGAGGTTTACTTGCATGTGCAGACAAACAACGAAGATGCCATTAACTTTTATAAGAAATTTGAGTTTGAAATTACAGAAACTATCCAGAACTATTACGTAAACATCACACCACCAGACTGTTATGTTGTCACAAGGCACATAACACCTAGCCCAACAAAGAAATAA
Protein sequence

>Msa0826810

MGAGREVSISLDGVRDKNIMQLKKLNLALFPVRYNDKYYADALASADFTKLAYYSDICVGAIACRLEKKEGGQVRVYIMTLGVLAPYRGLGIGTRLLNHVIDLCAKQNISEVYLHVQTNNEDAINFYKKFEFEITETIQNYYVNITPPDCYVVTRHITPSPTKK*