AlfalfaGEDB | Msa0828490

M. sativa cultivar zhongmu-4 / Msa0828490

KEGG: K12412
GO: -
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828490	RHN66613.1	81.347	193	29	4	1	187	1	192	2.85e-96	287

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828490	sp\|Q9FKK2\|AGL62_ARATH	31.757	148	92	4	36	177	26	170	2.36e-15	75.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828490	A0A396IQ06	81.347	193	29	4	1	187	1	192	1.36e-96	287

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TFs/TRs:

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Gene ID	Type	Classification
Msa0828490	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828490	MtrunA17_Chr3g0093131	81.347	193	29	4	1	187	1	192	2.62e-100	287
Msa0828490	MtrunA17_Chr2g0302091	61.081	185	64	6	1	181	16	196	8.87e-59	182
Msa0828490	MtrunA17_Chr8g0345001	50.857	175	73	6	15	180	10	180	6.68e-47	152
Msa0828490	MtrunA17_Chr2g0333981	36.196	163	96	3	12	168	1	161	2.06e-24	94.0
Msa0828490	MtrunA17_Chr4g0018951	36.842	133	74	2	36	168	31	153	3.91e-22	87.8
Msa0828490	MtrunA17_Chr8g0379021	35.135	148	88	3	24	165	13	158	9.85e-20	82.0
Msa0828490	MtrunA17_Chr5g0422421	36.129	155	87	4	21	165	7	159	5.53e-18	78.6
Msa0828490	MtrunA17_Chr4g0071601	34.507	142	83	3	36	168	31	171	8.21e-18	77.0
Msa0828490	MtrunA17_Chr3g0099421	32.911	158	88	3	21	165	32	184	7.06e-16	73.2
Msa0828490	MtrunA17_Chr8g0355091	30.909	165	95	4	14	165	1	159	7.29e-16	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0828490	AT5G60440.1	31.757	148	92	4	36	177	26	170	2.40e-16	75.5
Msa0828490	AT2G24840.1	28.090	178	106	6	8	175	63	228	3.31e-16	74.7
Msa0828490	AT3G66656.1	51.020	49	24	0	36	84	21	69	4.11e-12	62.0
Msa0828490	AT1G65360.1	29.747	158	92	5	21	165	5	156	8.74e-12	62.0
Msa0828490	AT4G36590.1	28.571	161	97	5	21	165	5	163	1.49e-11	61.6
Msa0828490	AT2G34440.1	29.508	122	80	2	36	154	21	139	3.35e-11	59.7

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Find 26 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAAGTTCTATGCATG+TGG	0.619639	5_4:-1074232	Msa0828490:CDS
ACATGCATAGAACTTGTTGT+TGG	0.594378	5_4:+1074234	None:intergenic
AGGAAAACTTAAAGAAGAGA+AGG	0.524858	5_4:-1074086	Msa0828490:CDS
CAACACACGGAAGAGAAAGA+AGG	0.527351	5_4:-1074371	Msa0828490:CDS
CATGCATAGAACTTGTTGTT+GGG	0.522753	5_4:+1074235	None:intergenic
CCCTTCTCAAAACTCAAACT+TGG	0.422835	5_4:-1074325	Msa0828490:CDS
CGATGAGTGCAGTTTCTGAT+TGG	0.423120	5_4:+1074266	None:intergenic
CGCTTCATATCGAAGTCTTA+GGG	0.401643	5_4:+1074114	None:intergenic
CGGCCGAGAGAAACAACACA+CGG	0.685523	5_4:-1074384	Msa0828490:CDS
CTCATCCTCGTCCATATGTC+CGG	0.445428	5_4:+1074171	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTCTAAGAAGAAAATTCCAA+TGG	-	chr5_4:1073906-1073925	Msa0828490:CDS	30.0%
TAAGAAGAAAATTCCAATGG+CGG	-	chr5_4:1073909-1073928	Msa0828490:CDS	30.0%
AGGAAAACTTAAAGAAGAGA+AGG	-	chr5_4:1074227-1074246	Msa0828490:CDS	30.0%
TAACACACATTCTCTTGAAT+TGG	+	chr5_4:1074429-1074448	None:intergenic	30.0%
GAAGGATGAAATCAAGAAAG+AGG	-	chr5_4:1073960-1073979	Msa0828490:CDS	35.0%
AACAACAAGTTCTATGCATG+TGG	-	chr5_4:1074081-1074100	Msa0828490:CDS	35.0%
GAATGACAGCATCATAGTTA+GGG	+	chr5_4:1074110-1074129	None:intergenic	35.0%
TGAATGACAGCATCATAGTT+AGG	+	chr5_4:1074111-1074130	None:intergenic	35.0%
CCCTTCTCAAAACTCAAACT+TGG	-	chr5_4:1073988-1074007	Msa0828490:CDS	40.0%
CGCTTCATATCGAAGTCTTA+GGG	+	chr5_4:1074202-1074221	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	1073886	1074449	1073886	ID=Msa0828490;Name=Msa0828490
chr5_4	mRNA	1073886	1074449	1073886	ID=Msa0828490-mRNA-1;Parent=Msa0828490;Name=Msa0828490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|187
chr5_4	exon	1073886	1074449	1073886	ID=Msa0828490-mRNA-1:exon:216;Parent=Msa0828490-mRNA-1
chr5_4	CDS	1073886	1074449	1073886	ID=Msa0828490-mRNA-1:cds;Parent=Msa0828490-mRNA-1

Gene Sequence

>Msa0828490

ATGAATAAGAATGGTGAAACGTCTAAGAAGAAAATTCCAATGGCGGCCGAGAGAAACAACACACGGAAGAGAAAGAAGGATGAAATCAAGAAAGAGGAAAAGCCCTTCTCAAAACTCAAACTTGGAATTTTCAACAAAGCAATAGAGCTATCTATTCTATGCCAATCAGAAACTGCACTCATCGTAACATCACCCAACAACAAGTTCTATGCATGTGGCTACCCTAACTATGATGCTGTCATTCAGCGTTTTCTGACCGGACATATGGACGAGGATGAGAAAAAGAAGCAAGAAGATGATGACGTTGTTGAAACCCTAAGACTTCGATATGAAGCGATTCAAGGAAAACTTAAAGAAGAGAAGGAGAAATTGCAAGCTGTCAAAGATGCACACAAGAGTAATTTTGATTTGCCTGCTGATTGGTGGAATAAATCTATTGATGATATGGATTTAACATCTCTTGAAGATATTACGACTTCGTTGAATAAATTGAAGATTAATTTGTCCGTAGCAAGCGAAGCGAAGAAGTTCAATTTACATCCAATTCAAGAGAATGTGTGTTAA

Protein sequence

>Msa0828490

MNKNGETSKKKIPMAAERNNTRKRKKDEIKKEEKPFSKLKLGIFNKAIELSILCQSETALIVTSPNNKFYACGYPNYDAVIQRFLTGHMDEDEKKKQEDDDVVETLRLRYEAIQGKLKEEKEKLQAVKDAHKSNFDLPADWWNKSIDDMDLTSLEDITTSLNKLKINLSVASEAKKFNLHPIQENVC*