AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833430


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833430 MtrunA17_Chr5g0399261 85.461 282 21 4 1 282 5 266 3.18e-169 469
Msa0833430 MtrunA17_Chr4g0056091 42.724 323 118 13 1 279 1 300 3.15e-58 188
Msa0833430 MtrunA17_Chr7g0231181 32.184 348 157 12 1 280 1 337 5.89e-38 137
Msa0833430 MtrunA17_Chr4g0012691 28.399 331 133 11 1 259 2 300 2.38e-23 98.2
Msa0833430 MtrunA17_Chr4g0018761 27.138 269 141 12 6 260 9 236 3.25e-16 76.3
Msa0833430 MtrunA17_Chr3g0079061 28.125 288 154 12 6 264 8 271 1.36e-13 69.3
Msa0833430 MtrunA17_Chr3g0079131 24.483 290 160 11 3 260 5 267 2.21e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833430 AT3G60580.1 29.755 326 133 12 1 280 1 276 1.04e-25 103
Msa0833430 AT4G16610.1 34.536 194 94 5 3 191 4 169 1.17e-21 90.5
Msa0833430 AT1G02030.1 28.144 334 108 16 2 282 3 257 2.57e-21 90.9
Msa0833430 AT2G45120.1 30.526 285 152 8 1 259 1 265 1.03e-19 87.4

Find 43 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTACTACTACTGTTTGT+TGG 0.284162 5_4:-6555769 None:intergenic
GTTATAGCAGCTTCTTCTTC+AGG 0.287165 5_4:-6556064 None:intergenic
AGAAGATGAAAATGAGAGTA+TGG 0.289041 5_4:-6555729 None:intergenic
GTGAGATTGTGTTTGTGGTT+GGG 0.304906 5_4:-6555687 None:intergenic
TTTGGTTCTTATCAAGCATT+AGG 0.305236 5_4:+6556313 Msa0833430:CDS
TGATGCTATCAAGAGATAAA+TGG 0.312199 5_4:+6556092 Msa0833430:CDS
TGAGATTGTGTTTGTGGTTG+GGG 0.323538 5_4:-6555686 None:intergenic
AAAAGTGAAGTAACAGGTTC+TGG 0.326984 5_4:-6556031 None:intergenic
TCTGGTTCAGTTTCTGTTAG+TGG 0.341539 5_4:-6556013 None:intergenic
GGTGAGATTGTGTTTGTGGT+TGG 0.402930 5_4:-6555688 None:intergenic
TGTTGTTGAGAAAGAAAACT+TGG 0.417388 5_4:-6555864 None:intergenic
GTGTGGTGAGATTGTGTTTG+TGG 0.419011 5_4:-6555692 None:intergenic
ATGAAAAGAGTCTAATGTAT+AGG 0.419874 5_4:+6555801 Msa0833430:CDS
TTGAGTTGGGTTCTTTGACT+TGG 0.422339 5_4:-6555918 None:intergenic
TCTTCTTCCAAGATGGATAG+AGG 0.427918 5_4:-6556354 None:intergenic
AATCTCACCTCTATCCATCT+TGG 0.444489 5_4:+6556347 Msa0833430:CDS
TTCATCAATGGTAAAGCATT+GGG 0.449808 5_4:+6555614 Msa0833430:CDS
TTATAGCAGCTTCTTCTTCA+GGG 0.455323 5_4:-6556063 None:intergenic
ATTATAAATGTGACAAATGT+GGG 0.460652 5_4:+6556161 Msa0833430:CDS
GAAGAAGAACAAGAAGTTTG+TGG 0.463316 5_4:+6556136 Msa0833430:CDS
AAGAAGAACAAGAAGTTTGT+GGG 0.463576 5_4:+6556137 Msa0833430:CDS
ATTCATCAATGGTAAAGCAT+TGG 0.467655 5_4:+6555613 Msa0833430:CDS
TGCTCAAGAACATTCATCAA+TGG 0.467969 5_4:+6555602 Msa0833430:CDS
TGGAAGAGGAAGAGGGAGGG+TGG 0.478080 5_4:-6555660 None:intergenic
GAGAGTATGGTAGAAGAAGT+AGG 0.483653 5_4:-6555716 None:intergenic
CTTTAGAAGTTCACAAGCAA+TGG 0.490495 5_4:+6556192 Msa0833430:CDS
GGTTTGGAAGAGGAAGAGGG+AGG 0.491265 5_4:-6555664 None:intergenic
AAGTTGAAAAGTGAAGTAAC+AGG 0.534456 5_4:-6556037 None:intergenic
GTTGTTGAGAAAGAAAACTT+GGG 0.543214 5_4:-6555863 None:intergenic
GGATCATCTTCTTATGATGA+AGG 0.547481 5_4:-6556415 None:intergenic
TGGTAGAAGAAGTAGGAGTG+TGG 0.559870 5_4:-6555709 None:intergenic
AATTATAAATGTGACAAATG+TGG 0.560495 5_4:+6556160 Msa0833430:CDS
GTTTGGAAGAGGAAGAGGGA+GGG 0.560895 5_4:-6555663 None:intergenic
AGCATTGGGTGGACACATGA+AGG 0.563543 5_4:+6555628 Msa0833430:CDS
GAGATTGTGTTTGTGGTTGG+GGG 0.564499 5_4:-6555685 None:intergenic
TATAGCAGCTTCTTCTTCAG+GGG 0.571162 5_4:-6556062 None:intergenic
CCTTAGGACAAAACACACAT+TGG 0.573593 5_4:-6556288 None:intergenic
ATCTCTTGATAGCATCATGA+GGG 0.581541 5_4:-6556086 None:intergenic
GGTTCTTATCAAGCATTAGG+TGG 0.596035 5_4:+6556316 Msa0833430:CDS
ATCAATGGTAAAGCATTGGG+TGG 0.611558 5_4:+6555617 Msa0833430:CDS
TATCTCTTGATAGCATCATG+AGG 0.628708 5_4:-6556087 None:intergenic
GTAGTTAGTGCCAAATGGAG+AGG 0.644078 5_4:+6555564 None:intergenic
TCATCTTCTTATGATGAAGG+TGG 0.658652 5_4:-6556412 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAAGTTTGAAACTTTTTTT+GGG - chr5_4:6555839-6555858 None:intergenic 20.0%
!!! TGAAAGTTTGAAACTTTTTT+TGG - chr5_4:6555840-6555859 None:intergenic 20.0%
!! AATTATAAATGTGACAAATG+TGG + chr5_4:6556160-6556179 Msa0833430:CDS 20.0%
!! ATTATAAATGTGACAAATGT+GGG + chr5_4:6556161-6556180 Msa0833430:CDS 20.0%
!!! ATTGGAATATTTTATGATCA+CGG - chr5_4:6556273-6556292 None:intergenic 20.0%
!!! GAAAGTTTGAAACTTTTTTT+GGG - chr5_4:6555839-6555858 None:intergenic 20.0%
!!! TGAAAGTTTGAAACTTTTTT+TGG - chr5_4:6555840-6555859 None:intergenic 20.0%
!! AATTATAAATGTGACAAATG+TGG + chr5_4:6556160-6556179 Msa0833430:CDS 20.0%
!! ATTATAAATGTGACAAATGT+GGG + chr5_4:6556161-6556180 Msa0833430:CDS 20.0%
!!! ATTGGAATATTTTATGATCA+CGG - chr5_4:6556273-6556292 None:intergenic 20.0%
! ATGAAAAGAGTCTAATGTAT+AGG + chr5_4:6555801-6555820 Msa0833430:CDS 25.0%
! ATGAAAAGAGTCTAATGTAT+AGG + chr5_4:6555801-6555820 Msa0833430:CDS 25.0%
ATTCATCAATGGTAAAGCAT+TGG + chr5_4:6555613-6555632 Msa0833430:CDS 30.0%
! TTCATCAATGGTAAAGCATT+GGG + chr5_4:6555614-6555633 Msa0833430:CDS 30.0%
AGAAGATGAAAATGAGAGTA+TGG - chr5_4:6555732-6555751 None:intergenic 30.0%
TTTGTACTACTACTGTTTGT+TGG - chr5_4:6555772-6555791 None:intergenic 30.0%
GTTGTTGAGAAAGAAAACTT+GGG - chr5_4:6555866-6555885 None:intergenic 30.0%
TGTTGTTGAGAAAGAAAACT+TGG - chr5_4:6555867-6555886 None:intergenic 30.0%
!!! TGAGTTTTCGTTTTTGAGTT+GGG - chr5_4:6555934-6555953 None:intergenic 30.0%
!! TTGTTCTGAATTTTGGACAA+AGG - chr5_4:6555957-6555976 None:intergenic 30.0%
!! GCTTCTTTTGTTCTGAATTT+TGG - chr5_4:6555964-6555983 None:intergenic 30.0%
AAGTTGAAAAGTGAAGTAAC+AGG - chr5_4:6556040-6556059 None:intergenic 30.0%
TGATGCTATCAAGAGATAAA+TGG + chr5_4:6556092-6556111 Msa0833430:CDS 30.0%
AAGAAGAACAAGAAGTTTGT+GGG + chr5_4:6556137-6556156 Msa0833430:CDS 30.0%
TGATAAGAACCAAAAACCTT+AGG - chr5_4:6556307-6556326 None:intergenic 30.0%
!! TTTGGTTCTTATCAAGCATT+AGG + chr5_4:6556313-6556332 Msa0833430:CDS 30.0%
ATTCATCAATGGTAAAGCAT+TGG + chr5_4:6555613-6555632 Msa0833430:CDS 30.0%
! TTCATCAATGGTAAAGCATT+GGG + chr5_4:6555614-6555633 Msa0833430:CDS 30.0%
AGAAGATGAAAATGAGAGTA+TGG - chr5_4:6555732-6555751 None:intergenic 30.0%
TTTGTACTACTACTGTTTGT+TGG - chr5_4:6555772-6555791 None:intergenic 30.0%
GTTGTTGAGAAAGAAAACTT+GGG - chr5_4:6555866-6555885 None:intergenic 30.0%
TGTTGTTGAGAAAGAAAACT+TGG - chr5_4:6555867-6555886 None:intergenic 30.0%
!!! TGAGTTTTCGTTTTTGAGTT+GGG - chr5_4:6555934-6555953 None:intergenic 30.0%
!! TTGTTCTGAATTTTGGACAA+AGG - chr5_4:6555957-6555976 None:intergenic 30.0%
!! GCTTCTTTTGTTCTGAATTT+TGG - chr5_4:6555964-6555983 None:intergenic 30.0%
AAGTTGAAAAGTGAAGTAAC+AGG - chr5_4:6556040-6556059 None:intergenic 30.0%
TGATGCTATCAAGAGATAAA+TGG + chr5_4:6556092-6556111 Msa0833430:CDS 30.0%
AAGAAGAACAAGAAGTTTGT+GGG + chr5_4:6556137-6556156 Msa0833430:CDS 30.0%
TGATAAGAACCAAAAACCTT+AGG - chr5_4:6556307-6556326 None:intergenic 30.0%
!! TTTGGTTCTTATCAAGCATT+AGG + chr5_4:6556313-6556332 Msa0833430:CDS 30.0%
TGCTCAAGAACATTCATCAA+TGG + chr5_4:6555602-6555621 Msa0833430:CDS 35.0%
!!! GTGAGTTTTCGTTTTTGAGT+TGG - chr5_4:6555935-6555954 None:intergenic 35.0%
AAAAGTGAAGTAACAGGTTC+TGG - chr5_4:6556034-6556053 None:intergenic 35.0%
TTATAGCAGCTTCTTCTTCA+GGG - chr5_4:6556066-6556085 None:intergenic 35.0%
! ATCTCTTGATAGCATCATGA+GGG - chr5_4:6556089-6556108 None:intergenic 35.0%
! TATCTCTTGATAGCATCATG+AGG - chr5_4:6556090-6556109 None:intergenic 35.0%
GAAGAAGAACAAGAAGTTTG+TGG + chr5_4:6556136-6556155 Msa0833430:CDS 35.0%
! CTTTAGAAGTTCACAAGCAA+TGG + chr5_4:6556192-6556211 Msa0833430:CDS 35.0%
! GTGTTTTGTCCTAAGGTTTT+TGG + chr5_4:6556295-6556314 Msa0833430:CDS 35.0%
TTCTTCTTCTTCTTCCAAGA+TGG - chr5_4:6556364-6556383 None:intergenic 35.0%
TGCTCAAGAACATTCATCAA+TGG + chr5_4:6555602-6555621 Msa0833430:CDS 35.0%
!!! GTGAGTTTTCGTTTTTGAGT+TGG - chr5_4:6555935-6555954 None:intergenic 35.0%
AAAAGTGAAGTAACAGGTTC+TGG - chr5_4:6556034-6556053 None:intergenic 35.0%
TTATAGCAGCTTCTTCTTCA+GGG - chr5_4:6556066-6556085 None:intergenic 35.0%
! ATCTCTTGATAGCATCATGA+GGG - chr5_4:6556089-6556108 None:intergenic 35.0%
! TATCTCTTGATAGCATCATG+AGG - chr5_4:6556090-6556109 None:intergenic 35.0%
GAAGAAGAACAAGAAGTTTG+TGG + chr5_4:6556136-6556155 Msa0833430:CDS 35.0%
! CTTTAGAAGTTCACAAGCAA+TGG + chr5_4:6556192-6556211 Msa0833430:CDS 35.0%
! GTGTTTTGTCCTAAGGTTTT+TGG + chr5_4:6556295-6556314 Msa0833430:CDS 35.0%
TTCTTCTTCTTCTTCCAAGA+TGG - chr5_4:6556364-6556383 None:intergenic 35.0%
!! ATCAATGGTAAAGCATTGGG+TGG + chr5_4:6555617-6555636 Msa0833430:CDS 40.0%
TGAGATTGTGTTTGTGGTTG+GGG - chr5_4:6555689-6555708 None:intergenic 40.0%
GTGAGATTGTGTTTGTGGTT+GGG - chr5_4:6555690-6555709 None:intergenic 40.0%
! GAGAGTATGGTAGAAGAAGT+AGG - chr5_4:6555719-6555738 None:intergenic 40.0%
!! TTGAGTTGGGTTCTTTGACT+TGG - chr5_4:6555921-6555940 None:intergenic 40.0%
! TCTGGTTCAGTTTCTGTTAG+TGG - chr5_4:6556016-6556035 None:intergenic 40.0%
TATAGCAGCTTCTTCTTCAG+GGG - chr5_4:6556065-6556084 None:intergenic 40.0%
GTTATAGCAGCTTCTTCTTC+AGG - chr5_4:6556067-6556086 None:intergenic 40.0%
CCTTAGGACAAAACACACAT+TGG - chr5_4:6556291-6556310 None:intergenic 40.0%
! CCAATGTGTGTTTTGTCCTA+AGG + chr5_4:6556288-6556307 Msa0833430:CDS 40.0%
GGTTCTTATCAAGCATTAGG+TGG + chr5_4:6556316-6556335 Msa0833430:CDS 40.0%
AATCTCACCTCTATCCATCT+TGG + chr5_4:6556347-6556366 Msa0833430:CDS 40.0%
TCTTCTTCCAAGATGGATAG+AGG - chr5_4:6556357-6556376 None:intergenic 40.0%
!! ATCAATGGTAAAGCATTGGG+TGG + chr5_4:6555617-6555636 Msa0833430:CDS 40.0%
TGAGATTGTGTTTGTGGTTG+GGG - chr5_4:6555689-6555708 None:intergenic 40.0%
GTGAGATTGTGTTTGTGGTT+GGG - chr5_4:6555690-6555709 None:intergenic 40.0%
! GAGAGTATGGTAGAAGAAGT+AGG - chr5_4:6555719-6555738 None:intergenic 40.0%
!! TTGAGTTGGGTTCTTTGACT+TGG - chr5_4:6555921-6555940 None:intergenic 40.0%
! TCTGGTTCAGTTTCTGTTAG+TGG - chr5_4:6556016-6556035 None:intergenic 40.0%
TATAGCAGCTTCTTCTTCAG+GGG - chr5_4:6556065-6556084 None:intergenic 40.0%
GTTATAGCAGCTTCTTCTTC+AGG - chr5_4:6556067-6556086 None:intergenic 40.0%
CCTTAGGACAAAACACACAT+TGG - chr5_4:6556291-6556310 None:intergenic 40.0%
! CCAATGTGTGTTTTGTCCTA+AGG + chr5_4:6556288-6556307 Msa0833430:CDS 40.0%
GGTTCTTATCAAGCATTAGG+TGG + chr5_4:6556316-6556335 Msa0833430:CDS 40.0%
AATCTCACCTCTATCCATCT+TGG + chr5_4:6556347-6556366 Msa0833430:CDS 40.0%
TCTTCTTCCAAGATGGATAG+AGG - chr5_4:6556357-6556376 None:intergenic 40.0%
GAGATTGTGTTTGTGGTTGG+GGG - chr5_4:6555688-6555707 None:intergenic 45.0%
GGTGAGATTGTGTTTGTGGT+TGG - chr5_4:6555691-6555710 None:intergenic 45.0%
GTGTGGTGAGATTGTGTTTG+TGG - chr5_4:6555695-6555714 None:intergenic 45.0%
TGGTAGAAGAAGTAGGAGTG+TGG - chr5_4:6555712-6555731 None:intergenic 45.0%
GAGATTGTGTTTGTGGTTGG+GGG - chr5_4:6555688-6555707 None:intergenic 45.0%
GGTGAGATTGTGTTTGTGGT+TGG - chr5_4:6555691-6555710 None:intergenic 45.0%
GTGTGGTGAGATTGTGTTTG+TGG - chr5_4:6555695-6555714 None:intergenic 45.0%
TGGTAGAAGAAGTAGGAGTG+TGG - chr5_4:6555712-6555731 None:intergenic 45.0%
! AGCATTGGGTGGACACATGA+AGG + chr5_4:6555628-6555647 Msa0833430:CDS 50.0%
GTTTGGAAGAGGAAGAGGGA+GGG - chr5_4:6555666-6555685 None:intergenic 50.0%
TGTGTTTGTGGTTGGGGGTT+TGG - chr5_4:6555683-6555702 None:intergenic 50.0%
! AGCATTGGGTGGACACATGA+AGG + chr5_4:6555628-6555647 Msa0833430:CDS 50.0%
GTTTGGAAGAGGAAGAGGGA+GGG - chr5_4:6555666-6555685 None:intergenic 50.0%
TGTGTTTGTGGTTGGGGGTT+TGG - chr5_4:6555683-6555702 None:intergenic 50.0%
GGTTTGGAAGAGGAAGAGGG+AGG - chr5_4:6555667-6555686 None:intergenic 55.0%
GGGGGTTTGGAAGAGGAAGA+GGG - chr5_4:6555670-6555689 None:intergenic 55.0%
TGGGGGTTTGGAAGAGGAAG+AGG - chr5_4:6555671-6555690 None:intergenic 55.0%
TGTGGTTGGGGGTTTGGAAG+AGG - chr5_4:6555677-6555696 None:intergenic 55.0%
GGTTTGGAAGAGGAAGAGGG+AGG - chr5_4:6555667-6555686 None:intergenic 55.0%
GGGGGTTTGGAAGAGGAAGA+GGG - chr5_4:6555670-6555689 None:intergenic 55.0%
TGGGGGTTTGGAAGAGGAAG+AGG - chr5_4:6555671-6555690 None:intergenic 55.0%
TGTGGTTGGGGGTTTGGAAG+AGG - chr5_4:6555677-6555696 None:intergenic 55.0%
TGGAAGAGGAAGAGGGAGGG+TGG - chr5_4:6555663-6555682 None:intergenic 60.0%
TGGAAGAGGAAGAGGGAGGG+TGG - chr5_4:6555663-6555682 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6555578 6556426 6555578 ID=Msa0833430;Name=Msa0833430
chr5_4 mRNA 6555578 6556426 6555578 ID=Msa0833430-mRNA-1;Parent=Msa0833430;Name=Msa0833430-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|282
chr5_4 exon 6555578 6556426 6555578 ID=Msa0833430-mRNA-1:exon:2834;Parent=Msa0833430-mRNA-1
chr5_4 CDS 6555578 6556426 6555578 ID=Msa0833430-mRNA-1:cds;Parent=Msa0833430-mRNA-1
Gene Sequence

>Msa0833430

ATGGAGAGGTACAAATGCAAGCTTTGCTCAAGAACATTCATCAATGGTAAAGCATTGGGTGGACACATGAAGGCTCATTTTGCCACCCTCCCTCTTCCTCTTCCAAACCCCCAACCACAAACACAATCTCACCACACTCCTACTTCTTCTACCATACTCTCATTTTCATCTTCTTCTGAAAATGAACAAGACCAACAAACAGTAGTAGTACAAAGAGACTCAAATGAAAAGAGTCTAATGTATAGGTTGAGAGAAAATCCCAAAAAAAGTTTCAAACTTTCAGATCCCAAGTTTTCTTTCTCAACAACAGAGACTATTGTTCATGACAGAGAGAGTGAAACCAAGTCAAAGAACCCAACTCAAAAACGAAAACTCACCTTTGTCCAAAATTCAGAACAAAAGAAGCTTAAACAAAGTTTGAAAGATTCACGTTCACCACTAACAGAAACTGAACCAGAACCTGTTACTTCACTTTTCAACTTTTCCCCTGAAGAAGAAGCTGCTATAACCCTCATGATGCTATCAAGAGATAAATGGAAGATCAATGTTGCTGTCAAAGAAGAAGAACAAGAAGTTTGTGGGAATTATAAATGTGACAAATGTGGGAATAATAACTTTAGAAGTTCACAAGCAATGGAAAGTCACAAAAGTATTTGTCTTCAAAATGAAACAAATCTTGCTTCAACAAGTAGCCGTGATCATAAAATATTCCAATGTGTGTTTTGTCCTAAGGTTTTTGGTTCTTATCAAGCATTAGGTGGTCACAAAAAATCTCACCTCTATCCATCTTGGAAGAAGAAGAAGAAGAAGCTATGTTTTTTCGATCTCAACTTGCCACCTTCATCATAA

Protein sequence

>Msa0833430

MERYKCKLCSRTFINGKALGGHMKAHFATLPLPLPNPQPQTQSHHTPTSSTILSFSSSSENEQDQQTVVVQRDSNEKSLMYRLRENPKKSFKLSDPKFSFSTTETIVHDRESETKSKNPTQKRKLTFVQNSEQKKLKQSLKDSRSPLTETEPEPVTSLFNFSPEEEAAITLMMLSRDKWKINVAVKEEEQEVCGNYKCDKCGNNNFRSSQAMESHKSICLQNETNLASTSSRDHKIFQCVFCPKVFGSYQALGGHKKSHLYPSWKKKKKKLCFFDLNLPPSS*