AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0834380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0834380 MtrunA17_Chr5g0399851 82.069 145 24 1 25 169 2 144 3.65e-84 244
Msa0834380 MtrunA17_Chr5g0399861 66.484 182 51 2 1 180 1 174 5.28e-83 243
Msa0834380 MtrunA17_Chr5g0399881 71.930 171 37 4 1 169 1 162 2.55e-79 233
Msa0834380 MtrunA17_Chr2g0301131 44.937 158 47 5 33 164 102 245 4.13e-35 123
Msa0834380 MtrunA17_Chr4g0070271 35.294 136 61 3 33 167 90 199 1.01e-18 79.7
Msa0834380 MtrunA17_Chr2g0302041 35.088 114 67 3 42 148 56 169 1.05e-13 66.6
Msa0834380 MtrunA17_Chr4g0069721 33.333 117 59 3 42 145 43 153 1.81e-12 62.8
Msa0834380 MtrunA17_Chr8g0344481 30.252 119 82 1 29 146 90 208 2.32e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0834380 AT1G19180.2 40.876 137 72 5 36 167 52 184 1.31e-25 97.8
Msa0834380 AT1G19180.1 40.876 137 72 5 36 167 118 250 4.34e-25 97.8
Msa0834380 AT1G19180.3 40.876 137 72 5 36 167 118 250 4.34e-25 97.8
Msa0834380 AT1G74950.1 42.017 119 61 2 36 146 111 229 7.86e-24 94.7
Msa0834380 AT1G74950.2 40.541 111 58 2 36 138 111 221 3.77e-19 81.6
Msa0834380 AT5G20900.1 37.398 123 57 5 44 155 57 170 2.02e-13 65.9
Msa0834380 AT1G72450.1 35.652 115 64 3 39 147 101 211 2.09e-12 64.3
Msa0834380 AT1G17380.1 32.520 123 73 3 33 147 87 207 6.87e-12 62.8
Msa0834380 AT1G17380.2 32.540 126 69 3 33 147 58 178 8.05e-12 62.4
Msa0834380 AT1G70700.2 34.109 129 80 4 42 168 117 242 3.33e-11 60.5

Find 37 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACAAGAGGTTTCAAATT+TGG 0.130518 5_4:+7407389 Msa0834380:CDS
CCATCCACACCTGTTATTTA+TGG 0.168614 5_4:+7407804 Msa0834380:CDS
AGAGGATGTAGTTCCTTTCT+TGG 0.251859 5_4:-7407682 None:intergenic
CTACAAGAGGTTTCAAATTT+GGG 0.272831 5_4:+7407390 Msa0834380:CDS
AGCTTCACTTCATCGGTTCT+TGG 0.292747 5_4:+7407951 Msa0834380:CDS
GTTGGGTTACTTGTTTGATA+TGG 0.307437 5_4:-7411446 None:intergenic
CATCATAAAATATTGTCATT+TGG 0.312886 5_4:-7407599 None:intergenic
ATTCAGATTTACCAATGATT+AGG 0.314150 5_4:+7407926 Msa0834380:intron
ATCATAAAATATTGTCATTT+GGG 0.325400 5_4:-7407598 None:intergenic
ATTCATTAATTGCCTTCTTA+AGG 0.334385 5_4:-7411472 None:intergenic
TTGCCTTCTTAAGGTCTGTT+GGG 0.384826 5_4:-7411463 None:intergenic
TTTCAAATTTGGGACATTCC+AGG 0.386089 5_4:+7407400 Msa0834380:CDS
CTCGAGCTACATTCTGTTTG+TGG 0.391793 5_4:-7411526 None:intergenic
AGTGAAGCTTTCCTAATCAT+TGG 0.409424 5_4:-7407937 None:intergenic
ATTGCCTTCTTAAGGTCTGT+TGG 0.425409 5_4:-7411464 None:intergenic
GGAGCTTGTTGAAAATTCAT+AGG 0.431112 5_4:-7407357 None:intergenic
GAATTTCTAGCAAGAAATGA+TGG 0.446507 5_4:-7407759 None:intergenic
TTCATCGGTTCTTGGAGAAA+AGG 0.463618 5_4:+7407959 Msa0834380:CDS
TGGTACTTGAAGAGAATTGT+AGG 0.475609 5_4:-7407784 None:intergenic
TCCTCATCTCTTTCTACAAG+AGG 0.490417 5_4:+7407377 Msa0834380:CDS
ATCCATGTCATGGCTCTCCT+TGG 0.504600 5_4:+7411493 Msa0834380:CDS
ACATTCTGTTTGTGGTCTTG+TGG 0.510551 5_4:-7411518 None:intergenic
TTAGGAAAGCTTCACTTCAT+CGG 0.526716 5_4:+7407944 Msa0834380:CDS
AATGAAGGCTAATATTATCA+AGG 0.546904 5_4:+7407560 Msa0834380:CDS
TTTGTGGTCTTGTGGTGCCA+AGG 0.551456 5_4:-7411510 None:intergenic
TCTTGGAGAAAAGGAAGGAT+AGG 0.554767 5_4:+7407968 Msa0834380:CDS
TCGGTTCTTGGAGAAAAGGA+AGG 0.585705 5_4:+7407963 Msa0834380:CDS
TGCCAAGGAGAGCCATGACA+TGG 0.588458 5_4:-7411495 None:intergenic
ACCTCTTGTAGAAAGAGATG+AGG 0.589295 5_4:-7407378 None:intergenic
TTTCTACAGAATTTCTGCAA+GGG 0.590111 5_4:+7411421 Msa0834380:intron
CCATAAATAACAGGTGTGGA+TGG 0.595900 5_4:-7407804 None:intergenic
TTTACCATAAATAACAGGTG+TGG 0.596443 5_4:-7407808 None:intergenic
CAATTAATGAATCCATGTCA+TGG 0.606119 5_4:+7411483 Msa0834380:CDS
TAACCCAACAGACCTTAAGA+AGG 0.618363 5_4:+7411460 Msa0834380:CDS
GTTTCTACAGAATTTCTGCA+AGG 0.621041 5_4:+7411420 Msa0834380:intron
AATTTCTAGCAAGAAATGAT+GGG 0.637681 5_4:-7407758 None:intergenic
TTCTAGCAAGAAATGATGGG+TGG 0.652275 5_4:-7407755 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGGTAAAAAAAAAAAAAT+TGG + chr5_4:7407822-7407841 Msa0834380:intron 10.0%
!! AAATCTGAATATATATAAAT+AGG - chr5_4:7407916-7407935 None:intergenic 10.0%
!!! TATTTTTTAATTAAAATTCC+GGG + chr5_4:7408737-7408756 Msa0834380:intron 10.0%
!! ATATGAGTTTAATAAAAAAT+AGG - chr5_4:7409102-7409121 None:intergenic 10.0%
!!! TTTCAGAAATTTTAAAATTA+AGG - chr5_4:7409384-7409403 None:intergenic 10.0%
!! ATTATTAAAAATTCTGAATT+GGG - chr5_4:7409782-7409801 None:intergenic 10.0%
!! TATTATTAAAAATTCTGAAT+TGG - chr5_4:7409783-7409802 None:intergenic 10.0%
!!! TTCAGAATTTTTAATAATAT+AGG + chr5_4:7409784-7409803 Msa0834380:intron 10.0%
!! AAAATCATAATTAGAAAAAA+AGG - chr5_4:7411368-7411387 None:intergenic 10.0%
!!! ATCATAAAATATTGTCATTT+GGG - chr5_4:7407601-7407620 None:intergenic 15.0%
!!! AAATGACAATATTTTATGAT+GGG + chr5_4:7407601-7407620 Msa0834380:CDS 15.0%
!!! TTTTTTTTACCATAAATAAC+AGG - chr5_4:7407816-7407835 None:intergenic 15.0%
!! TTGATTGTAAACAAAAATAA+AGG + chr5_4:7408942-7408961 Msa0834380:intron 15.0%
!! TGATTGTAAACAAAAATAAA+GGG + chr5_4:7408943-7408962 Msa0834380:intron 15.0%
!!! GAAAAGTTGATATATTTATA+AGG - chr5_4:7408986-7409005 None:intergenic 15.0%
!!! AAAAGTGATAAAAAACTTTT+GGG - chr5_4:7409073-7409092 None:intergenic 15.0%
!!! AAAAAGTGATAAAAAACTTT+TGG - chr5_4:7409074-7409093 None:intergenic 15.0%
!! GACAAATTTCATTAAAAAAT+AGG + chr5_4:7409278-7409297 Msa0834380:intron 15.0%
!! TCTAGAAATTGATATAATAA+GGG - chr5_4:7409323-7409342 None:intergenic 15.0%
!! ATCTAGAAATTGATATAATA+AGG - chr5_4:7409324-7409343 None:intergenic 15.0%
!!! TTATATCAATTTCTAGATTT+TGG + chr5_4:7409326-7409345 Msa0834380:intron 15.0%
!! ATTGAAAAAAAAATTATCAC+CGG - chr5_4:7409556-7409575 None:intergenic 15.0%
!!! AAAAGTATGATACAAATTTT+AGG + chr5_4:7409965-7409984 Msa0834380:intron 15.0%
!! TAATTTATAACCGATAAATT+TGG + chr5_4:7410062-7410081 Msa0834380:intron 15.0%
!! AATCTTTAAATTAATCTCAA+CGG + chr5_4:7410260-7410279 Msa0834380:intron 15.0%
!!! AAAGTAATTTTGTGAAAATT+AGG + chr5_4:7410384-7410403 Msa0834380:intron 15.0%
!! ATTGTTAAATAATCATATGT+AGG - chr5_4:7410449-7410468 None:intergenic 15.0%
!!! ATTTCTTTTGGTTTAATTTT+TGG + chr5_4:7410530-7410549 Msa0834380:intron 15.0%
!!! TTTCTTTTGGTTTAATTTTT+GGG + chr5_4:7410531-7410550 Msa0834380:intron 15.0%
!! TATAGACTTATTTAATCTAA+TGG + chr5_4:7410883-7410902 Msa0834380:intron 15.0%
!! ATTATCAAATATAAGTTCAT+CGG - chr5_4:7410997-7411016 None:intergenic 15.0%
!!! TATTTGATAATTGATAAACT+AGG + chr5_4:7411006-7411025 Msa0834380:intron 15.0%
!! GTTATATTAATTATTGTTTG+TGG + chr5_4:7411028-7411047 Msa0834380:intron 15.0%
!! CATCATAAAATATTGTCATT+TGG - chr5_4:7407602-7407621 None:intergenic 20.0%
!!! CAAATGACAATATTTTATGA+TGG + chr5_4:7407600-7407619 Msa0834380:CDS 20.0%
!! ATAGGTATGTTGTAATAATT+AGG + chr5_4:7407986-7408005 Msa0834380:intron 20.0%
!! AAAAAGCAGTTACAAAAATT+TGG + chr5_4:7408653-7408672 Msa0834380:intron 20.0%
!! TATATTCAAAATGTACAACA+AGG + chr5_4:7408786-7408805 Msa0834380:intron 20.0%
!!! AAAGTGATAAAAAACTTTTG+GGG - chr5_4:7409072-7409091 None:intergenic 20.0%
!! TAATGAAATTTGTCAGAAAT+GGG - chr5_4:7409272-7409291 None:intergenic 20.0%
!! TTAATGAAATTTGTCAGAAA+TGG - chr5_4:7409273-7409292 None:intergenic 20.0%
!! CTAGAAATTGATATAATAAG+GGG - chr5_4:7409322-7409341 None:intergenic 20.0%
!! AATTAAGGTGATTAAAATTG+TGG - chr5_4:7409369-7409388 None:intergenic 20.0%
!! ATCACTCTTAATTTAAGTAT+TGG - chr5_4:7409413-7409432 None:intergenic 20.0%
!! AAAATTGAGGATGATAATTT+AGG + chr5_4:7409597-7409616 Msa0834380:intron 20.0%
!!! TTTCACCACATTTTTTTTTA+AGG + chr5_4:7409620-7409639 Msa0834380:intron 20.0%
!!! TTCACCACATTTTTTTTTAA+GGG + chr5_4:7409621-7409640 Msa0834380:intron 20.0%
!!! CTTTTATTTTAATGTTGAAG+TGG + chr5_4:7409682-7409701 Msa0834380:intron 20.0%
!! AAATTGCAGATTTAAAAACA+TGG + chr5_4:7409812-7409831 Msa0834380:intron 20.0%
!! AATTGCAGATTTAAAAACAT+GGG + chr5_4:7409813-7409832 Msa0834380:intron 20.0%
!!! TAATGTTAATTTCTGGATTT+TGG - chr5_4:7409841-7409860 None:intergenic 20.0%
!! AAATCCAGAAATTAACATTA+TGG + chr5_4:7409841-7409860 Msa0834380:intron 20.0%
!! AATCCAGAAATTAACATTAT+GGG + chr5_4:7409842-7409861 Msa0834380:intron 20.0%
!! ATTAAGCCTAAAAAATATGA+AGG + chr5_4:7409880-7409899 Msa0834380:intron 20.0%
!!! GTTTAACCTTCATATTTTTT+AGG - chr5_4:7409889-7409908 None:intergenic 20.0%
!!! TTATTCACTATTTCACTTTT+GGG - chr5_4:7410033-7410052 None:intergenic 20.0%
!!! ATTATTCACTATTTCACTTT+TGG - chr5_4:7410034-7410053 None:intergenic 20.0%
!! TACGAACTCAATTAATATAA+TGG - chr5_4:7410153-7410172 None:intergenic 20.0%
!! GAGATTAATTTAAAGATTGA+AGG - chr5_4:7410258-7410277 None:intergenic 20.0%
!!! TTTCACAAAATTACTTTTGT+TGG - chr5_4:7410381-7410400 None:intergenic 20.0%
!!! AGTTTAATAAAGTGTTTTGT+TGG - chr5_4:7410617-7410636 None:intergenic 20.0%
!! TTTATTAAACTACTTGATGA+AGG + chr5_4:7410626-7410645 Msa0834380:intron 20.0%
!! AGAAAATATAAGTTTATCAG+TGG - chr5_4:7410962-7410981 None:intergenic 20.0%
!! ATTATCTAACTTCAAATCAA+TGG - chr5_4:7411236-7411255 None:intergenic 20.0%
! AGTAGCTAAAAAAATGTTGA+TGG - chr5_4:7407460-7407479 None:intergenic 25.0%
! CATGATTCATGTTTATAACA+TGG + chr5_4:7407492-7407511 Msa0834380:intron 25.0%
!!! GTTTTATTACAGTGTAATGA+AGG + chr5_4:7407545-7407564 Msa0834380:intron 25.0%
! AATGAAGGCTAATATTATCA+AGG + chr5_4:7407560-7407579 Msa0834380:CDS 25.0%
!!! AATTTCTAGCAAGAAATGAT+GGG - chr5_4:7407761-7407780 None:intergenic 25.0%
! AAATTGGATATCTATTTGAG+TGG + chr5_4:7407838-7407857 Msa0834380:intron 25.0%
! ATTCAGATTTACCAATGATT+AGG + chr5_4:7407926-7407945 Msa0834380:intron 25.0%
! CAAAAATGTTGATATAGTTG+TGG - chr5_4:7408130-7408149 None:intergenic 25.0%
! TTCTTTATATCTCTTGAATG+CGG - chr5_4:7408635-7408654 None:intergenic 25.0%
!!! AACCACTAATTACTGTTTTT+TGG + chr5_4:7408844-7408863 Msa0834380:intron 25.0%
! TACAATCAAACAGTAATTAG+TGG - chr5_4:7408931-7408950 None:intergenic 25.0%
! AATTGTGGAATTAGAAACAT+GGG - chr5_4:7409354-7409373 None:intergenic 25.0%
! AAATTGTGGAATTAGAAACA+TGG - chr5_4:7409355-7409374 None:intergenic 25.0%
!!! TTTTTTTCAATAACCACTAG+TGG + chr5_4:7409565-7409584 Msa0834380:intron 25.0%
!!! TCACCACATTTTTTTTTAAG+GGG + chr5_4:7409622-7409641 Msa0834380:intron 25.0%
!! TATTTAAAATTGGAGTGATG+TGG - chr5_4:7409754-7409773 None:intergenic 25.0%
!! TTGGGCTCAATATTTAAAAT+TGG - chr5_4:7409764-7409783 None:intergenic 25.0%
! ATTGCAGATTTAAAAACATG+GGG + chr5_4:7409814-7409833 Msa0834380:intron 25.0%
! AAAATATGAAGGTTAAACAC+AGG + chr5_4:7409891-7409910 Msa0834380:intron 25.0%
! TTTATAACCGATAAATTTGG+TGG + chr5_4:7410065-7410084 Msa0834380:intron 25.0%
! TTAGAATCCACCAAATTTAT+CGG - chr5_4:7410075-7410094 None:intergenic 25.0%
! TTTAAAGATTGAAGGAATAG+AGG - chr5_4:7410250-7410269 None:intergenic 25.0%
!!! TTAATAAAGTGTTTTGTTGG+CGG - chr5_4:7410614-7410633 None:intergenic 25.0%
!! TTCAATCTTTTTGCAAAAGA+AGG - chr5_4:7411140-7411159 None:intergenic 25.0%
! TTTGCAAAAAGATTGAATCT+CGG + chr5_4:7411143-7411162 Msa0834380:intron 25.0%
! AATCAATAATTTCTGCACAT+AGG + chr5_4:7411259-7411278 Msa0834380:intron 25.0%
! AACAAAGATATCAGTAAATC+TGG - chr5_4:7411397-7411416 None:intergenic 25.0%
! ATTCATTAATTGCCTTCTTA+AGG - chr5_4:7411475-7411494 None:intergenic 25.0%
TCTACAAGAGGTTTCAAATT+TGG + chr5_4:7407389-7407408 Msa0834380:CDS 30.0%
CTACAAGAGGTTTCAAATTT+GGG + chr5_4:7407390-7407409 Msa0834380:CDS 30.0%
!! GAATTTCTAGCAAGAAATGA+TGG - chr5_4:7407762-7407781 None:intergenic 30.0%
TTTACCATAAATAACAGGTG+TGG - chr5_4:7407811-7407830 None:intergenic 30.0%
AATGTTGATATAGTTGTGGT+CGG - chr5_4:7408126-7408145 None:intergenic 30.0%
AATTACGCGAAAAATCCTTA+AGG - chr5_4:7408247-7408266 None:intergenic 30.0%
AGAATGGTCTGTTAAATCTT+GGG - chr5_4:7408282-7408301 None:intergenic 30.0%
AAGAATGGTCTGTTAAATCT+TGG - chr5_4:7408283-7408302 None:intergenic 30.0%
AAGATTTAACAGACCATTCT+TGG + chr5_4:7408282-7408301 Msa0834380:intron 30.0%
GTCTATATATGCATAACTCT+GGG - chr5_4:7408335-7408354 None:intergenic 30.0%
AGTCTATATATGCATAACTC+TGG - chr5_4:7408336-7408355 None:intergenic 30.0%
TAACTTCAAGCATAAAAGAG+AGG + chr5_4:7408377-7408396 Msa0834380:intron 30.0%
! TTTTATGTGCTACAGTTCAA+AGG + chr5_4:7408408-7408427 Msa0834380:intron 30.0%
! GTTTCAAATATTTTGTGTCC+CGG - chr5_4:7408758-7408777 None:intergenic 30.0%
! TCTTATTTGTTTGAGAGATC+TGG - chr5_4:7408816-7408835 None:intergenic 30.0%
CACCAAAAAACAGTAATTAG+TGG - chr5_4:7408849-7408868 None:intergenic 30.0%
!!! CACTAATTACTGTTTTTTGG+TGG + chr5_4:7408847-7408866 Msa0834380:intron 30.0%
!!! TAATTACTGTTTTTTGGTGG+TGG + chr5_4:7408850-7408869 Msa0834380:intron 30.0%
ATTGTGGAATTAGAAACATG+GGG - chr5_4:7409353-7409372 None:intergenic 30.0%
TGATTTGAATGCCATATCAA+TGG + chr5_4:7409434-7409453 Msa0834380:intron 30.0%
GTTAAGTAAAACGAATCCAA+TGG + chr5_4:7409475-7409494 Msa0834380:intron 30.0%
GAAAAAAAAATTATCACCGG+AGG - chr5_4:7409553-7409572 None:intergenic 30.0%
!! GATAAATTTGGTGGATTCTA+AGG + chr5_4:7410074-7410093 Msa0834380:intron 30.0%
! ATGGCTCTTATTGTTATTCA+TGG - chr5_4:7410134-7410153 None:intergenic 30.0%
TAAATGTGATTCAGCGTATA+TGG - chr5_4:7410190-7410209 None:intergenic 30.0%
!!! TTACCCTTTTTCAACATTCA+GGG - chr5_4:7410679-7410698 None:intergenic 30.0%
AAAGGGTAAGCAAATTGAAA+TGG + chr5_4:7410690-7410709 Msa0834380:intron 30.0%
AAGGGTAAGCAAATTGAAAT+GGG + chr5_4:7410691-7410710 Msa0834380:intron 30.0%
! AAGTTTATCAGTGGATTCTA+GGG - chr5_4:7410953-7410972 None:intergenic 30.0%
! TAAGTTTATCAGTGGATTCT+AGG - chr5_4:7410954-7410973 None:intergenic 30.0%
! AGTTAGTCTGAAAACTTAAG+AGG + chr5_4:7411056-7411075 Msa0834380:intron 30.0%
!!! CTCAAACTTTTTTTTTGTGC+TGG + chr5_4:7411098-7411117 Msa0834380:intron 30.0%
! AAGTGAAGGGATAATTTTCT+TGG - chr5_4:7411307-7411326 None:intergenic 30.0%
ATTTCGAAACAAGAAGTGAA+GGG - chr5_4:7411320-7411339 None:intergenic 30.0%
TATTTCGAAACAAGAAGTGA+AGG - chr5_4:7411321-7411340 None:intergenic 30.0%
TTTCTACAGAATTTCTGCAA+GGG + chr5_4:7411421-7411440 Msa0834380:intron 30.0%
CAATTAATGAATCCATGTCA+TGG + chr5_4:7411483-7411502 Msa0834380:CDS 30.0%
!! GGAGCTTGTTGAAAATTCAT+AGG - chr5_4:7407360-7407379 None:intergenic 35.0%
! TTTCAAATTTGGGACATTCC+AGG + chr5_4:7407400-7407419 Msa0834380:CDS 35.0%
TGAAAAAGTCAACGACATCA+TGG + chr5_4:7407653-7407672 Msa0834380:CDS 35.0%
! ATCATGGTCTTTTCCAAGAA+AGG + chr5_4:7407669-7407688 Msa0834380:CDS 35.0%
! AGTTGTTGTTGTTGTTGTTG+TGG - chr5_4:7407713-7407732 None:intergenic 35.0%
TGGTACTTGAAGAGAATTGT+AGG - chr5_4:7407787-7407806 None:intergenic 35.0%
AGTGAAGCTTTCCTAATCAT+TGG - chr5_4:7407940-7407959 None:intergenic 35.0%
TTAGGAAAGCTTCACTTCAT+CGG + chr5_4:7407944-7407963 Msa0834380:CDS 35.0%
! TTGATAATCGATTCAGTAGC+AGG - chr5_4:7408167-7408186 None:intergenic 35.0%
!!! TCACTGCTTGTTTGTTTTAC+AGG - chr5_4:7408192-7408211 None:intergenic 35.0%
AATGGTCTGTTAAATCTTGG+GGG - chr5_4:7408280-7408299 None:intergenic 35.0%
GAATGGTCTGTTAAATCTTG+GGG - chr5_4:7408281-7408300 None:intergenic 35.0%
!!! CCTTGGTTTTAAACCAAGAA+TGG - chr5_4:7408298-7408317 None:intergenic 35.0%
! CCATTCTTGGTTTAAAACCA+AGG + chr5_4:7408295-7408314 Msa0834380:intron 35.0%
! TCTTGGTTTAAAACCAAGGA+TGG + chr5_4:7408299-7408318 Msa0834380:intron 35.0%
TTGACGTGAACAAACAAAAG+GGG - chr5_4:7408460-7408479 None:intergenic 35.0%
GTTGACGTGAACAAACAAAA+GGG - chr5_4:7408461-7408480 None:intergenic 35.0%
TGTTGACGTGAACAAACAAA+AGG - chr5_4:7408462-7408481 None:intergenic 35.0%
! GGATTCGTTTTACTTAACGA+AGG - chr5_4:7409473-7409492 None:intergenic 35.0%
!! ATCCAATGGCTTGTTAGAAA+AGG + chr5_4:7409489-7409508 Msa0834380:intron 35.0%
! TTTTCATTCATCACCACTAG+TGG - chr5_4:7409581-7409600 None:intergenic 35.0%
! GTGGTGATGAATGAAAATTG+AGG + chr5_4:7409584-7409603 Msa0834380:intron 35.0%
TCGCCCCTTAAAAAAAAATG+TGG - chr5_4:7409628-7409647 None:intergenic 35.0%
CCTCCCATAATGTTAATTTC+TGG - chr5_4:7409848-7409867 None:intergenic 35.0%
CCAGAAATTAACATTATGGG+AGG + chr5_4:7409845-7409864 Msa0834380:intron 35.0%
! GCTCTTATTGTTATTCATGG+TGG - chr5_4:7410131-7410150 None:intergenic 35.0%
AAGGAATAGAGGAGATAAAG+GGG - chr5_4:7410239-7410258 None:intergenic 35.0%
GAAGGAATAGAGGAGATAAA+GGG - chr5_4:7410240-7410259 None:intergenic 35.0%
TGAAGGAATAGAGGAGATAA+AGG - chr5_4:7410241-7410260 None:intergenic 35.0%
! CAATCTTGTGCTTTTGTTGA+TGG + chr5_4:7410465-7410484 Msa0834380:intron 35.0%
!! AACGAAAGTTGGTGTATTCA+AGG - chr5_4:7410497-7410516 None:intergenic 35.0%
! CTACAAGTTCTAACGAAAGT+TGG - chr5_4:7410508-7410527 None:intergenic 35.0%
CAACTTTCGTTAGAACTTGT+AGG + chr5_4:7410506-7410525 Msa0834380:intron 35.0%
! GAACTTGTAGGCATTTCTTT+TGG + chr5_4:7410518-7410537 Msa0834380:intron 35.0%
ACCAATACACTACTAGAACA+TGG + chr5_4:7410580-7410599 Msa0834380:intron 35.0%
ACCATGTTCTAGTAGTGTAT+TGG - chr5_4:7410584-7410603 None:intergenic 35.0%
!!! CTTACCCTTTTTCAACATTC+AGG - chr5_4:7410680-7410699 None:intergenic 35.0%
AAACGCTTAGCAAATTCAGA+AGG - chr5_4:7410737-7410756 None:intergenic 35.0%
!! TGAATTTGCTAAGCGTTTCT+TGG + chr5_4:7410739-7410758 Msa0834380:intron 35.0%
TAAGCGTTTCTTGGAACAAA+TGG + chr5_4:7410748-7410767 Msa0834380:intron 35.0%
! ACACAAAGTGAGATTTTGCT+AGG + chr5_4:7410800-7410819 Msa0834380:intron 35.0%
! CACAAAGTGAGATTTTGCTA+GGG + chr5_4:7410801-7410820 Msa0834380:intron 35.0%
GGTCAATTAAAGTGATACAC+CGG + chr5_4:7410904-7410923 Msa0834380:intron 35.0%
TTTCGAAATAGTTTGAGTCG+TGG + chr5_4:7411331-7411350 Msa0834380:intron 35.0%
GTTTCTACAGAATTTCTGCA+AGG + chr5_4:7411420-7411439 Msa0834380:intron 35.0%
GTTGGGTTACTTGTTTGATA+TGG - chr5_4:7411449-7411468 None:intergenic 35.0%
TCCTCATCTCTTTCTACAAG+AGG + chr5_4:7407377-7407396 Msa0834380:CDS 40.0%
ACCTCTTGTAGAAAGAGATG+AGG - chr5_4:7407381-7407400 None:intergenic 40.0%
GTAGCAAAAGAACATGTACC+TGG - chr5_4:7407421-7407440 None:intergenic 40.0%
!!! ATGTCGTTGACTTTTTCAGC+TGG - chr5_4:7407651-7407670 None:intergenic 40.0%
AGAGGATGTAGTTCCTTTCT+TGG - chr5_4:7407685-7407704 None:intergenic 40.0%
!!! GTTGTTGTTGTGGTTTTGAG+AGG - chr5_4:7407703-7407722 None:intergenic 40.0%
! TTCTAGCAAGAAATGATGGG+TGG - chr5_4:7407758-7407777 None:intergenic 40.0%
CCATAAATAACAGGTGTGGA+TGG - chr5_4:7407807-7407826 None:intergenic 40.0%
! CCATCCACACCTGTTATTTA+TGG + chr5_4:7407804-7407823 Msa0834380:CDS 40.0%
!! TTCATCGGTTCTTGGAGAAA+AGG + chr5_4:7407959-7407978 Msa0834380:CDS 40.0%
TCTTGGAGAAAAGGAAGGAT+AGG + chr5_4:7407968-7407987 Msa0834380:CDS 40.0%
TGACGTGAACAAACAAAAGG+GGG - chr5_4:7408459-7408478 None:intergenic 40.0%
! GACCTTTTCTAACAAGCCAT+TGG - chr5_4:7409494-7409513 None:intergenic 40.0%
AGAAAAGGTCGATGGAATGA+TGG + chr5_4:7409504-7409523 Msa0834380:intron 40.0%
AGAAATAGCGATGAAACCTC+CGG + chr5_4:7409534-7409553 Msa0834380:intron 40.0%
TGGCATGTTTGGATATGACA+TGG - chr5_4:7409734-7409753 None:intergenic 40.0%
!! ATTTGGTGGATTCTAAGGTG+AGG + chr5_4:7410079-7410098 Msa0834380:intron 40.0%
TCAATTAAGTCTCTCACCAG+TGG + chr5_4:7410104-7410123 Msa0834380:intron 40.0%
ATTAATCTCAACGGCCTGAA+TGG + chr5_4:7410269-7410288 Msa0834380:intron 40.0%
TGTTACCATTACCACCATTC+AGG - chr5_4:7410286-7410305 None:intergenic 40.0%
!! AAAGTGTTTTGTTGGCGGTT+TGG - chr5_4:7410609-7410628 None:intergenic 40.0%
TGCACCCTGAATGTTGAAAA+AGG + chr5_4:7410672-7410691 Msa0834380:intron 40.0%
GCACCCTGAATGTTGAAAAA+GGG + chr5_4:7410673-7410692 Msa0834380:intron 40.0%
ATCTCACTTTGTGTTGCACT+AGG - chr5_4:7410794-7410813 None:intergenic 40.0%
ATTAAAGTGATACACCGGTG+AGG + chr5_4:7410909-7410928 Msa0834380:intron 40.0%
TTAAAGTGATACACCGGTGA+GGG + chr5_4:7410910-7410929 Msa0834380:intron 40.0%
GCCATCAATTTGTGATTGGT+CGG + chr5_4:7411165-7411184 Msa0834380:intron 40.0%
TAACCCAACAGACCTTAAGA+AGG + chr5_4:7411460-7411479 Msa0834380:CDS 40.0%
TTGCCTTCTTAAGGTCTGTT+GGG - chr5_4:7411466-7411485 None:intergenic 40.0%
ATTGCCTTCTTAAGGTCTGT+TGG - chr5_4:7411467-7411486 None:intergenic 40.0%
! ACATTCTGTTTGTGGTCTTG+TGG - chr5_4:7411521-7411540 None:intergenic 40.0%
!! AGCTTCACTTCATCGGTTCT+TGG + chr5_4:7407951-7407970 Msa0834380:CDS 45.0%
TCGGTTCTTGGAGAAAAGGA+AGG + chr5_4:7407963-7407982 Msa0834380:CDS 45.0%
TGTTGATCGCACTGTCCTTA+AGG + chr5_4:7408229-7408248 Msa0834380:intron 45.0%
TGTGCTACAGTTCAAAGGCT+AGG + chr5_4:7408413-7408432 Msa0834380:intron 45.0%
!!! TACTGTTTTTTGGTGGTGGC+CGG + chr5_4:7408854-7408873 Msa0834380:intron 45.0%
CATCGCATGCTCCATTGATA+TGG - chr5_4:7409448-7409467 None:intergenic 45.0%
!! GGCTTGTTAGAAAAGGTCGA+TGG + chr5_4:7409496-7409515 Msa0834380:intron 45.0%
TTGGAGTGATGTGGCATGTT+TGG - chr5_4:7409745-7409764 None:intergenic 45.0%
TGTTATTCATGGTGGTCCAC+TGG - chr5_4:7410123-7410142 None:intergenic 45.0%
AGATAAAGGGGAGAAAGAGG+GGG - chr5_4:7410227-7410246 None:intergenic 45.0%
GAGATAAAGGGGAGAAAGAG+GGG - chr5_4:7410228-7410247 None:intergenic 45.0%
GGAGATAAAGGGGAGAAAGA+GGG - chr5_4:7410229-7410248 None:intergenic 45.0%
AGGAGATAAAGGGGAGAAAG+AGG - chr5_4:7410230-7410249 None:intergenic 45.0%
CAACATTCAGGGTGCAAATG+TGG - chr5_4:7410668-7410687 None:intergenic 45.0%
TTTGTGTTGCACTAGGACCA+TGG - chr5_4:7410787-7410806 None:intergenic 45.0%
CTAGAGAAAGAGAGAGAGAG+AGG + chr5_4:7410824-7410843 Msa0834380:intron 45.0%
!! ATCAGTGGATTCTAGGGTGA+GGG - chr5_4:7410947-7410966 None:intergenic 45.0%
!! TATCAGTGGATTCTAGGGTG+AGG - chr5_4:7410948-7410967 None:intergenic 45.0%
CTCGGCCATCAATTTGTGAT+TGG + chr5_4:7411161-7411180 Msa0834380:intron 45.0%
GCCGACCAATCACAAATTGA+TGG - chr5_4:7411169-7411188 None:intergenic 45.0%
CTCGAGCTACATTCTGTTTG+TGG - chr5_4:7411529-7411548 None:intergenic 45.0%
!!! ATATTTTTTAATTAAAATTC+CGG + chr5_4:7408736-7408755 Msa0834380:intron 5.0%
!! AAAAATTTACAAAAATTAAA+AGG - chr5_4:7409008-7409027 None:intergenic 5.0%
!!! ATTTTATTAAAAAAATATGA+AGG - chr5_4:7409129-7409148 None:intergenic 5.0%
!!! TTAAAAATATCTATTTTTTT+TGG + chr5_4:7409224-7409243 Msa0834380:intron 5.0%
!!! TAAAAATATCTATTTTTTTT+GGG + chr5_4:7409225-7409244 Msa0834380:intron 5.0%
GGGTTCTCATCTTCCATCCT+TGG - chr5_4:7408315-7408334 None:intergenic 50.0%
!!! ACTGTTTTTTGGTGGTGGCC+GGG + chr5_4:7408855-7408874 Msa0834380:intron 50.0%
! TCAGTTGGTATGCAAGCTCC+GGG - chr5_4:7408889-7408908 None:intergenic 50.0%
! CTCAGTTGGTATGCAAGCTC+CGG - chr5_4:7408890-7408909 None:intergenic 50.0%
TCTCGTGAGCTTAGCTCAGT+TGG - chr5_4:7408904-7408923 None:intergenic 50.0%
! GGTCGATGGAATGATGGTGA+TGG + chr5_4:7409510-7409529 Msa0834380:intron 50.0%
AATCTCAACGGCCTGAATGG+TGG + chr5_4:7410272-7410291 Msa0834380:intron 50.0%
AACGGCCTGAATGGTGGTAA+TGG + chr5_4:7410278-7410297 Msa0834380:intron 50.0%
AGAGGAGACATGATCGAGTG+TGG + chr5_4:7410842-7410861 Msa0834380:intron 50.0%
GGCTCAATTAAGTCCCTCAC+CGG - chr5_4:7410926-7410945 None:intergenic 50.0%
ATCCATGTCATGGCTCTCCT+TGG + chr5_4:7411493-7411512 Msa0834380:CDS 50.0%
!! TTTGTGGTCTTGTGGTGCCA+AGG - chr5_4:7411513-7411532 None:intergenic 50.0%
!!! CTGTTTTTTGGTGGTGGCCG+GGG + chr5_4:7408856-7408875 Msa0834380:intron 55.0%
! CAGTTGGTATGCAAGCTCCG+GGG - chr5_4:7408888-7408907 None:intergenic 55.0%
ATGGAGACCAAGCTCTGCCA+TGG + chr5_4:7410767-7410786 Msa0834380:intron 55.0%
ACTAGGACCATGGCAGAGCT+TGG - chr5_4:7410777-7410796 None:intergenic 55.0%
TGCCAAGGAGAGCCATGACA+TGG - chr5_4:7411498-7411517 None:intergenic 55.0%
AAGCTCCGGGGTTCGAACCC+CGG - chr5_4:7408876-7408895 None:intergenic 65.0%
GGGGAGAAAGAGGGGGAGAG+AGG - chr5_4:7410220-7410239 None:intergenic 65.0%
GGTGGCCGGGGTTCGAACCC+CGG + chr5_4:7408868-7408887 Msa0834380:intron 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 7407336 7411568 7407336 ID=Msa0834380;Name=Msa0834380
chr5_4 mRNA 7407336 7411568 7407336 ID=Msa0834380-mRNA-1;Parent=Msa0834380;Name=Msa0834380-mRNA-1;_AED=0.46;_eAED=0.47;_QI=0|0|0|1|1|1|4|0|183
chr5_4 exon 7407336 7407421 7407336 ID=Msa0834380-mRNA-1:exon:3330;Parent=Msa0834380-mRNA-1
chr5_4 exon 7407557 7407825 7407557 ID=Msa0834380-mRNA-1:exon:3331;Parent=Msa0834380-mRNA-1
chr5_4 exon 7407932 7407989 7407932 ID=Msa0834380-mRNA-1:exon:3332;Parent=Msa0834380-mRNA-1
chr5_4 exon 7411430 7411568 7411430 ID=Msa0834380-mRNA-1:exon:3333;Parent=Msa0834380-mRNA-1
chr5_4 CDS 7407336 7407421 7407336 ID=Msa0834380-mRNA-1:cds;Parent=Msa0834380-mRNA-1
chr5_4 CDS 7407557 7407825 7407557 ID=Msa0834380-mRNA-1:cds;Parent=Msa0834380-mRNA-1
chr5_4 CDS 7407932 7407989 7407932 ID=Msa0834380-mRNA-1:cds;Parent=Msa0834380-mRNA-1
chr5_4 CDS 7411430 7411568 7411430 ID=Msa0834380-mRNA-1:cds;Parent=Msa0834380-mRNA-1
Gene Sequence

>Msa0834380

ATGAATCACTACAAACGTACACCTATGAATTTTCAACAAGCTCCTCATCTCTTTCTACAAGAGGTTTCAAATTTGGGACATTCCAGTGTAATGAAGGCTAATATTATCAAGGAAGAGCTTAGAAGTGCCCAAATGACAATATTTTATGATGGGAAAGTAATTGTGTTTGATGATATTCCAGCTGAAAAAGTCAACGACATCATGGTCTTTTCCAAGAAAGGAACTACATCCTCTCAAAACCACAACAACAACAACAACAACTATGCTTTCAGATTTGCTCAGAGCCACCCATCATTTCTTGCTAGAAATTCTGCCTACAATTCTCTTCAAGTACCATCCACACCTGTTATTTATGATTTACCAATGATTAGGAAAGCTTCACTTCATCGGTTCTTGGAGAAAAGGAAGGATAGAATTTCTGCAAGGGCACCATATCAAACAAGTAACCCAACAGACCTTAAGAAGGCAATTAATGAATCCATGTCATGGCTCTCCTTGGCACCACAAGACCACAAACAGAATGTAGCTCGAGCTCTCACTACAAGAAAGTGA
Protein sequence

>Msa0834380

MNHYKRTPMNFQQAPHLFLQEVSNLGHSSVMKANIIKEELRSAQMTIFYDGKVIVFDDIPAEKVNDIMVFSKKGTTSSQNHNNNNNNYAFRFAQSHPSFLARNSAYNSLQVPSTPVIYDLPMIRKASLHRFLEKRKDRISARAPYQTSNPTDLKKAINESMSWLSLAPQDHKQNVARALTTRK*