AlfalfaGEDB | Msa0835290

M. sativa cultivar zhongmu-4 / Msa0835290

KEGG: K10523
GO: GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031461 GO:0031625 GO:0032991 GO:0043161 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234
PFAM: PF00651.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0835290	KYP73506.1	50.467	107	50	2	5	110	150	254	2.33e-22	100

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0835290	sp\|Q9XHZ8\|Y1178_ARATH	40.625	128	71	3	2	129	151	273	1.14e-19	85.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0835290	A0A151U2K6	50.467	107	50	2	5	110	150	254	1.11e-22	100

TFs/TRs:

Gene ID	Type	Classification
Msa0835290	TR	TRAF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0835290	MtrunA17_Chr5g0447191	47.059	102	53	1	4	105	18	118	2.20e-24	92.4
Msa0835290	MtrunA17_Chr3g0103031	44.954	109	57	2	2	110	155	260	3.52e-21	86.7
Msa0835290	MtrunA17_Chr3g0124921	41.818	110	61	2	20	129	167	273	2.27e-18	79.3
Msa0835290	MtrunA17_Chr5g0447111	37.037	108	66	2	2	109	155	260	1.01e-17	77.4
Msa0835290	MtrunA17_Chr1g0159831	40.000	110	63	2	20	129	173	279	6.35e-17	75.1
Msa0835290	MtrunA17_Chr4g0029841	39.560	91	50	1	13	103	122	207	3.35e-13	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0835290	AT1G21780.2	40.625	128	71	3	2	129	151	273	1.16e-20	85.9
Msa0835290	AT1G21780.1	40.625	128	71	3	2	129	151	273	1.16e-20	85.9
Msa0835290	AT1G55760.1	44.231	104	55	2	2	105	154	254	2.90e-20	84.7

Find 12 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTAATTTCTGAGAAGAAAA+AGG	0.240544	5_4:+8596073	Msa0835290:CDS
CTTAACAGACGTCGTAATTA+AGG	0.308709	5_4:+8595974	Msa0835290:CDS
AAAGTATATGTGTGAGCTAT+TGG	0.345442	5_4:+8596193	Msa0835290:CDS
AGAGCTCATAAATGTGTTCT+TGG	0.346517	5_4:+8596020	Msa0835290:CDS
TCAGGTATGTTTATAGTAGT+TGG	0.378921	5_4:-8596101	None:intergenic
AGCATATGAAATTGCTGACT+TGG	0.432729	5_4:+8596226	Msa0835290:CDS
GTTCGCTCAAGTGTTAAGAT+TGG	0.452194	5_4:+8595947	Msa0835290:CDS
AACTTTCATAGAGATACTTA+AGG	0.471143	5_4:-8596304	None:intergenic
TGAAATTGCTGACTTGGAAA+AGG	0.515198	5_4:+8596232	Msa0835290:CDS
CAAACTTCAAATGACATGTC+AGG	0.548382	5_4:-8596119	None:intergenic
ATAAATGTGTTCTTGGTGCA+CGG	0.593241	5_4:+8596027	Msa0835290:CDS
ACAGACGTCGTAATTAAGGT+CGG	0.697400	5_4:+8595978	Msa0835290:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTAATTTCTGAGAAGAAAA+AGG	+	chr5_4:8596073-8596092	Msa0835290:CDS	25.0%
!	AACTTTCATAGAGATACTTA+AGG	-	chr5_4:8596307-8596326	None:intergenic	25.0%
	TCAGGTATGTTTATAGTAGT+TGG	-	chr5_4:8596104-8596123	None:intergenic	30.0%
	AAAGTATATGTGTGAGCTAT+TGG	+	chr5_4:8596193-8596212	Msa0835290:CDS	30.0%
!!	AAGTTTTGTAAAGATCTCGT+TGG	+	chr5_4:8596322-8596341	Msa0835290:CDS	30.0%
	CTTAACAGACGTCGTAATTA+AGG	+	chr5_4:8595974-8595993	Msa0835290:CDS	35.0%
	AGAGCTCATAAATGTGTTCT+TGG	+	chr5_4:8596020-8596039	Msa0835290:CDS	35.0%
!	ATAAATGTGTTCTTGGTGCA+CGG	+	chr5_4:8596027-8596046	Msa0835290:CDS	35.0%
	CAAACTTCAAATGACATGTC+AGG	-	chr5_4:8596122-8596141	None:intergenic	35.0%
!	AGCATATGAAATTGCTGACT+TGG	+	chr5_4:8596226-8596245	Msa0835290:CDS	35.0%
!!	TGAAATTGCTGACTTGGAAA+AGG	+	chr5_4:8596232-8596251	Msa0835290:CDS	35.0%
	GTTCGCTCAAGTGTTAAGAT+TGG	+	chr5_4:8595947-8595966	Msa0835290:CDS	40.0%
	ACAGACGTCGTAATTAAGGT+CGG	+	chr5_4:8595978-8595997	Msa0835290:CDS	40.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	8595945	8596352	8595945	ID=Msa0835290;Name=Msa0835290
chr5_4	mRNA	8595945	8596352	8595945	ID=Msa0835290-mRNA-1;Parent=Msa0835290;Name=Msa0835290-mRNA-1;_AED=0.10;_eAED=0.12;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|135
chr5_4	exon	8595945	8596352	8595945	ID=Msa0835290-mRNA-1:exon:3810;Parent=Msa0835290-mRNA-1
chr5_4	CDS	8595945	8596352	8595945	ID=Msa0835290-mRNA-1:cds;Parent=Msa0835290-mRNA-1

Gene Sequence

>Msa0835290

ATGTTCGCTCAAGTGTTAAGATTGGATGTCTTAACAGACGTCGTAATTAAGGTCGGTGCATCTGATAAAAGCATAAGAGCTCATAAATGTGTTCTTGGTGCACGGTCAGCAGTTGTAGATCGCATGTTTGTAATTTCTGAGAAGAAAAAGGAACAACCAACTACTATAAACATACCTGACATGTCATTTGAAGTTTGTCAAGCATTAATCAGTTATATGTATGATGACATTGAAGAAGAAGAATTTAGAAAGTATATGTGTGAGCTATTGGATGCTGCTAAAGCATATGAAATTGCTGACTTGGAAAAGGCGTGTAGTGAAGTGATTTTTATTATTCTTGTTATTTCTTTTGTCAATTTCCTTAAGTATCTCTATGAAAGTTTTGTAAAGATCTCGTTGGAACTCTAG

Protein sequence

>Msa0835290

MFAQVLRLDVLTDVVIKVGASDKSIRAHKCVLGARSAVVDRMFVISEKKKEQPTTINIPDMSFEVCQALISYMYDDIEEEEFRKYMCELLDAAKAYEIADLEKACSEVIFIILVISFVNFLKYLYESFVKISLEL*