AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0835480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835480 MtrunA17_Chr5g0401421 94.196 448 26 0 22 469 1 448 0.0 857
Msa0835480 MtrunA17_Chr4g0053901 66.461 486 130 11 1 469 1 470 0.0 606
Msa0835480 MtrunA17_Chr2g0323111 48.293 205 92 2 265 469 118 308 3.11e-62 205
Msa0835480 MtrunA17_Chr2g0323111 41.441 111 50 3 49 154 45 145 1.04e-15 78.2
Msa0835480 MtrunA17_Chr5g0401131 27.033 418 260 9 56 468 114 491 1.08e-42 157
Msa0835480 MtrunA17_Chr3g0125811 27.251 422 245 8 54 466 201 569 1.91e-40 152
Msa0835480 MtrunA17_Chr1g0159461 26.506 415 257 6 54 466 223 591 1.60e-39 150
Msa0835480 MtrunA17_Chr2g0329591 26.959 434 243 13 55 468 73 452 3.72e-36 139
Msa0835480 MtrunA17_Chr2g0319091 26.478 423 249 10 53 466 209 578 1.27e-35 139
Msa0835480 MtrunA17_Chr5g0445331 24.773 440 261 11 42 466 147 531 1.37e-35 138
Msa0835480 MtrunA17_Chr2g0306641 26.202 416 248 11 56 466 125 486 3.23e-35 137
Msa0835480 MtrunA17_Chr3g0101141 25.000 424 254 11 53 466 172 541 8.86e-35 136
Msa0835480 MtrunA17_Chr5g0447691 25.754 431 242 12 53 466 174 543 5.23e-34 134
Msa0835480 MtrunA17_Chr8g0357601 26.077 418 252 12 57 466 183 551 4.38e-33 132
Msa0835480 MtrunA17_Chr4g0076091 25.986 431 244 13 53 467 172 543 1.12e-32 130
Msa0835480 MtrunA17_Chr8g0344101 25.935 428 253 12 54 466 221 599 1.61e-30 124
Msa0835480 MtrunA17_Chr8g0376381 26.202 416 237 13 57 466 170 521 1.62e-30 124
Msa0835480 MtrunA17_Chr4g0031711 23.934 422 272 10 53 469 358 735 3.82e-30 124
Msa0835480 MtrunA17_Chr3g0110971 25.935 428 238 10 57 466 220 586 8.68e-30 122
Msa0835480 MtrunA17_Chr2g0290241 25.476 420 258 11 53 466 54 424 2.69e-29 119
Msa0835480 MtrunA17_Chr7g0224271 25.060 419 260 10 56 466 300 672 2.79e-29 121
Msa0835480 MtrunA17_Chr4g0057891 27.358 424 244 15 55 466 148 519 9.06e-28 115
Msa0835480 MtrunA17_Chr4g0031731 24.821 419 267 15 53 467 251 625 2.85e-27 115
Msa0835480 MtrunA17_Chr2g0328391 22.406 424 282 9 49 469 292 671 2.09e-26 112
Msa0835480 MtrunA17_Chr4g0031701 24.344 419 269 14 53 467 251 625 5.09e-26 111
Msa0835480 MtrunA17_Chr4g0031721 24.882 422 264 12 53 469 313 686 9.38e-26 110
Msa0835480 MtrunA17_Chr4g0057291 22.857 420 269 12 56 468 292 663 1.66e-24 107
Msa0835480 MtrunA17_Chr7g0236851 24.946 465 265 13 9 466 269 656 1.64e-23 103
Msa0835480 MtrunA17_Chr3g0085681 24.312 436 278 11 57 466 26 435 2.55e-23 102
Msa0835480 MtrunA17_Chr4g0031741 23.866 419 264 12 56 469 284 652 1.33e-22 101
Msa0835480 MtrunA17_Chr6g0468561 24.648 426 255 11 52 466 291 661 2.63e-22 100
Msa0835480 MtrunA17_Chr4g0039661 26.366 421 251 13 51 466 188 554 5.94e-22 99.0
Msa0835480 MtrunA17_Chr7g0238891 21.905 420 279 9 53 469 369 742 1.08e-21 98.6
Msa0835480 MtrunA17_Chr7g0245601 21.602 412 262 11 59 468 441 793 1.11e-21 98.6
Msa0835480 MtrunA17_Chr2g0328341 22.275 422 273 11 53 469 269 640 1.11e-21 98.2
Msa0835480 MtrunA17_Chr5g0396491 23.377 462 263 13 56 466 56 477 2.04e-20 93.6
Msa0835480 MtrunA17_Chr2g0328381 21.801 422 275 11 53 469 272 643 3.88e-20 93.6
Msa0835480 MtrunA17_Chr4g0038851 22.887 485 291 15 30 466 19 468 4.35e-20 92.8
Msa0835480 MtrunA17_Chr2g0328431 21.739 437 268 12 48 469 279 656 7.36e-20 92.8
Msa0835480 MtrunA17_Chr3g0085361 24.496 347 222 9 145 466 18 349 1.35e-19 90.1
Msa0835480 MtrunA17_Chr1g0183641 22.169 415 258 12 56 466 123 476 3.03e-19 90.1
Msa0835480 MtrunA17_Chr2g0328411 21.739 414 274 11 56 467 289 654 6.23e-19 89.7
Msa0835480 MtrunA17_Chr4g0038761 23.373 415 257 14 59 469 87 444 2.19e-18 87.4
Msa0835480 MtrunA17_Chr2g0328401 22.449 392 251 9 48 435 280 622 1.61e-17 85.5
Msa0835480 MtrunA17_Chr8g0383981 23.598 428 245 14 56 469 139 498 9.02e-17 82.8
Msa0835480 MtrunA17_Chr1g0200401 23.009 452 251 15 43 466 76 458 1.40e-14 75.9
Msa0835480 MtrunA17_Chr5g0424131 23.168 423 268 15 56 466 121 498 1.04e-13 73.2
Msa0835480 MtrunA17_Chr3g0114841 21.646 328 207 7 147 466 214 499 1.12e-13 73.2
Msa0835480 MtrunA17_Chr7g0270031 22.910 323 200 9 150 466 274 553 3.56e-13 71.6
Msa0835480 MtrunA17_Chr2g0328371 22.472 267 164 8 150 412 13 240 4.81e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0835480 AT3G49950.1 56.293 437 151 9 35 468 10 409 3.09e-165 472
Msa0835480 AT5G67411.1 55.066 227 75 4 184 408 1 202 9.61e-75 233
Msa0835480 AT4G37650.1 27.716 451 268 12 35 469 123 531 4.63e-43 159
Msa0835480 AT5G48150.1 26.988 415 250 7 57 466 123 489 9.84e-34 133
Msa0835480 AT5G48150.4 26.988 415 250 7 57 466 123 489 9.84e-34 133
Msa0835480 AT5G48150.2 26.988 415 250 7 57 466 123 489 9.84e-34 133
Msa0835480 AT5G48150.3 26.988 415 250 7 57 466 123 489 9.84e-34 133
Msa0835480 AT1G21450.1 26.241 423 249 9 54 466 223 592 1.14e-33 134
Msa0835480 AT5G17490.1 26.087 414 247 6 55 466 159 515 2.59e-33 132
Msa0835480 AT2G04890.1 25.000 416 257 7 53 466 50 412 5.94e-33 129
Msa0835480 AT1G50600.3 24.645 422 258 9 53 466 156 525 2.81e-32 129
Msa0835480 AT1G50600.1 25.118 422 256 10 53 466 227 596 3.26e-32 130
Msa0835480 AT1G50600.2 25.118 422 256 10 53 466 231 600 4.12e-32 129
Msa0835480 AT3G03450.1 26.214 412 251 8 57 466 184 544 5.06e-32 129
Msa0835480 AT4G17230.1 24.009 429 256 9 53 468 155 526 7.36e-30 122
Msa0835480 AT4G17230.2 24.009 429 256 9 53 468 155 526 7.36e-30 122
Msa0835480 AT2G29065.1 26.077 418 266 11 56 469 252 630 1.61e-28 119
Msa0835480 AT2G01570.1 25.182 413 249 13 57 466 225 580 9.74e-28 116
Msa0835480 AT1G14920.1 24.578 415 249 12 57 466 173 528 6.33e-27 114
Msa0835480 AT3G13840.1 26.179 424 242 13 54 466 146 509 1.76e-26 112
Msa0835480 AT5G52510.1 22.811 434 258 11 52 466 264 639 4.01e-25 109
Msa0835480 AT1G66350.1 24.455 413 246 10 57 466 156 505 3.06e-24 105
Msa0835480 AT1G50420.1 24.123 456 267 14 56 466 57 478 3.28e-24 105
Msa0835480 AT2G37650.2 23.404 423 265 14 53 468 332 702 2.10e-22 100
Msa0835480 AT2G37650.1 23.404 423 265 14 53 468 344 714 2.31e-22 100
Msa0835480 AT1G55580.1 23.690 439 275 13 53 466 41 444 1.93e-21 97.1
Msa0835480 AT3G46600.2 23.333 420 272 11 53 469 1 373 1.06e-20 94.0
Msa0835480 AT5G59450.1 23.165 436 281 10 41 469 212 600 3.79e-20 94.0
Msa0835480 AT3G46600.1 23.239 426 265 11 53 469 209 581 3.92e-20 94.0
Msa0835480 AT3G46600.3 23.404 423 268 11 53 469 177 549 7.11e-20 92.8
Msa0835480 AT1G07530.1 21.140 421 282 11 53 469 393 767 6.21e-19 90.5
Msa0835480 AT1G63100.2 23.653 334 199 9 148 466 361 653 1.02e-18 89.7
Msa0835480 AT1G63100.1 23.653 334 199 9 148 466 336 628 1.02e-18 89.7
Msa0835480 AT2G29060.1 21.934 424 275 10 53 468 318 693 2.72e-18 88.2
Msa0835480 AT2G29060.2 21.934 424 275 10 53 468 281 656 2.86e-18 88.2
Msa0835480 AT5G66770.1 26.370 292 176 8 147 435 294 549 3.21e-17 84.7
Msa0835480 AT5G41920.1 22.782 417 256 14 56 466 43 399 4.51e-16 80.5
Msa0835480 AT3G50650.1 24.277 346 184 9 147 466 248 541 1.46e-12 70.1

Find 77 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCTTACGACGAATT+AGG 0.219981 5_4:+8848870 Msa0835480:CDS
CGACGAATTAGGTTCAAAAT+TGG 0.230348 5_4:+8848881 Msa0835480:CDS
AATCCGCGTTTAATTATCTT+TGG 0.234805 5_4:+8849249 Msa0835480:CDS
GCGGTTTGTGTAACTGCTTC+TGG 0.248870 5_4:-8848531 None:intergenic
GCAGCTGTTAGCTTGATTAA+AGG 0.266223 5_4:-8848792 None:intergenic
TTTCAGTGAGGATTCGGTTT+TGG 0.295734 5_4:+8849457 Msa0835480:CDS
AGATCAAGCATTGGTGGAAT+TGG 0.305832 5_4:-8848852 None:intergenic
TAAAACAAGAAACGTGATTA+TGG 0.317994 5_4:+8848914 Msa0835480:CDS
AAGTGTAGTAGAGGGTTATT+AGG 0.328746 5_4:-8848309 None:intergenic
GCATTGGTGGAATTGGAATA+TGG 0.329573 5_4:-8848845 None:intergenic
ATGAGCTTCTTGTGCTTACT+TGG 0.341746 5_4:+8849534 Msa0835480:CDS
AATGTAAATAGAGTTTCTTC+AGG 0.361286 5_4:-8849071 None:intergenic
AGATTTAACATCAAGTAGTT+TGG 0.367518 5_4:+8849214 Msa0835480:CDS
CACTAGCACAACAAATCCTT+TGG 0.378101 5_4:+8848400 Msa0835480:CDS
GCTTCCTGCTAGGCGTTGAT+TGG 0.395425 5_4:-8848457 None:intergenic
TTAACACCTTGGCATCGTTT+CGG 0.442385 5_4:+8848633 Msa0835480:CDS
TCCACCAAGACGACTATTGT+TGG 0.444785 5_4:-8849182 None:intergenic
TCTCATTCGTTGCTCCCACT+TGG 0.447629 5_4:-8849411 None:intergenic
ATGATAGTTATAACTATTTG+CGG 0.450034 5_4:-8848550 None:intergenic
AGTTTGTTTCAGTGAGGATT+CGG 0.453807 5_4:+8849451 Msa0835480:CDS
GAACAAGGACTAATAACTAC+AGG 0.460908 5_4:-8848273 None:intergenic
TAATAAATCTCATGAAGCAT+TGG 0.465324 5_4:+8849016 Msa0835480:CDS
CTGAGAGTGTTGTGTTGTTA+AGG 0.465694 5_4:-8848425 None:intergenic
AAGTGTGTGGTTTGGTGAAA+AGG 0.471718 5_4:-8848219 None:intergenic
ATGTTATCTTTGCTTCTGCT+TGG 0.471769 5_4:+8849567 Msa0835480:CDS
TAGTAGAGGGTTATTAGGTG+TGG 0.478477 5_4:-8848304 None:intergenic
GGTATGAAGCTGAAATTTGT+TGG 0.481427 5_4:+8849327 Msa0835480:CDS
GCAATTCTAGAAGCTACTAA+AGG 0.483791 5_4:+8848675 Msa0835480:CDS
ATTGTTACTTTCAATTGCAC+TGG 0.486642 5_4:-8848373 None:intergenic
GTCTCCAATCAACGCCTAGC+AGG 0.489729 5_4:+8848453 Msa0835480:CDS
GAGTAACTGCTCCATGCAAT+TGG 0.490828 5_4:-8848340 None:intergenic
AGAAAACTCGTGTGTTTGTA+TGG 0.500579 5_4:-8848583 None:intergenic
CTTCTTGTGCTTACTTGGAA+AGG 0.503343 5_4:+8849539 Msa0835480:CDS
ATGCTTGATGTACATGCAGC+TGG 0.511507 5_4:+8849491 Msa0835480:CDS
TTCTGGAATATTGCACCTAC+AGG 0.516060 5_4:-8848514 None:intergenic
TGATGTACATGCAGCTGGAT+GGG 0.516728 5_4:+8849496 Msa0835480:CDS
GAGAATGTGATAGCATATGA+AGG 0.519545 5_4:+8849356 Msa0835480:CDS
AGATCCAACAATAGTCGTCT+TGG 0.519549 5_4:+8849178 Msa0835480:CDS
TCCAACAATAGTCGTCTTGG+TGG 0.526010 5_4:+8849181 Msa0835480:CDS
AATGTGTCCACCGTGTCAAA+CGG 0.536257 5_4:-8849275 None:intergenic
ACTAGCACAACAAATCCTTT+GGG 0.538819 5_4:+8848401 Msa0835480:CDS
GATGTACATGCAGCTGGATG+GGG 0.543723 5_4:+8849497 Msa0835480:CDS
GGTTGCAAAAGTGTGTGGTT+TGG 0.543864 5_4:-8848227 None:intergenic
CTTCAACAACGCCAATTGCA+TGG 0.549170 5_4:+8848329 Msa0835480:CDS
AGTAGAGGGTTATTAGGTGT+GGG 0.549560 5_4:-8848303 None:intergenic
CGTTGTTGAAGTGTAGTAGA+GGG 0.553431 5_4:-8848317 None:intergenic
GTGGGAGCAACGAATGAGAA+AGG 0.554477 5_4:+8849415 Msa0835480:CDS
TGAAGCTGAAATTTGTTGGA+AGG 0.560306 5_4:+8849331 Msa0835480:CDS
TAAGAAAGATCAAGCATTGG+TGG 0.560629 5_4:-8848858 None:intergenic
GTTTCTTCAACTTATTCAGA+CGG 0.564374 5_4:+8848948 Msa0835480:CDS
CTCGAATGAAGCTTCCTGCT+AGG 0.577569 5_4:-8848467 None:intergenic
TTGATGTACATGCAGCTGGA+TGG 0.580774 5_4:+8849495 Msa0835480:CDS
TTGTGTTGTTAAGGACCCAA+AGG 0.585571 5_4:-8848416 None:intergenic
GCAATGGCGTCGATTAGTGT+AGG 0.586251 5_4:-8848750 None:intergenic
TGCCTTTGTTTACTCCCTCG+TGG 0.588128 5_4:-8849302 None:intergenic
GCGTTGTTGAAGTGTAGTAG+AGG 0.604252 5_4:-8848318 None:intergenic
TGTGTGGTTTGGTGAAAAGG+TGG 0.606992 5_4:-8848216 None:intergenic
GCTGTTAGCTTGATTAAAGG+AGG 0.610301 5_4:-8848789 None:intergenic
AACCTCATGATGATTTGCAA+TGG 0.610435 5_4:-8848766 None:intergenic
CAGCTGCAGTTAATAACTGT+AGG 0.611302 5_4:+8848808 Msa0835480:CDS
AATGTTGCACTTGCACTCGT+AGG 0.611711 5_4:-8848992 None:intergenic
CGCCATTGCAAATCATCATG+AGG 0.616800 5_4:+8848764 Msa0835480:CDS
TTGGATACCGTTTGACACGG+TGG 0.625357 5_4:+8849268 Msa0835480:CDS
TCTTTGGATACCGTTTGACA+CGG 0.625561 5_4:+8849265 Msa0835480:CDS
AGAATGTGATAGCATATGAA+GGG 0.625664 5_4:+8849357 Msa0835480:CDS
GAGGGAGTAAACAAAGGCAG+TGG 0.640189 5_4:+8849306 Msa0835480:CDS
TCAAGGAGTTTGTTTCAGTG+AGG 0.641089 5_4:+8849445 Msa0835480:CDS
GGTGTGGGAAATTCTGAACA+AGG 0.649547 5_4:-8848288 None:intergenic
TCGTAAGAAAGATCAAGCAT+TGG 0.656089 5_4:-8848861 None:intergenic
GAGTCGAGTCTAAAACCAAG+TGG 0.658958 5_4:+8849396 Msa0835480:CDS
AACAAGGACTAATAACTACA+GGG 0.659957 5_4:-8848272 None:intergenic
AGTCGAGTCTAAAACCAAGT+GGG 0.662079 5_4:+8849397 Msa0835480:CDS
TACCACGAGGGAGTAAACAA+AGG 0.668759 5_4:+8849300 Msa0835480:CDS
GGTAGGGTTGCAAAAGTGTG+TGG 0.682842 5_4:-8848232 None:intergenic
GTGTAACCGAAACGATGCCA+AGG 0.704462 5_4:-8848639 None:intergenic
CGCGTGCAAGAAGATCCTGT+AGG 0.763698 5_4:+8848499 Msa0835480:CDS
TATCCAAAGATAATTAAACG+CGG 0.789557 5_4:-8849252 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAGTTATAACTATTTG+CGG - chr5_4:8848553-8848572 None:intergenic 20.0%
!!! ATTTTATTGATTTAACACCT+TGG + chr5_4:8848622-8848641 Msa0835480:CDS 20.0%
!! ATGATAGTTATAACTATTTG+CGG - chr5_4:8848553-8848572 None:intergenic 20.0%
!!! ATTTTATTGATTTAACACCT+TGG + chr5_4:8848622-8848641 Msa0835480:CDS 20.0%
!!! TTTGATTTTTGTTCATGGTA+GGG - chr5_4:8848251-8848270 None:intergenic 25.0%
!!! ATTTGATTTTTGTTCATGGT+AGG - chr5_4:8848252-8848271 None:intergenic 25.0%
! TAAAACAAGAAACGTGATTA+TGG + chr5_4:8848914-8848933 Msa0835480:CDS 25.0%
! TAATAAATCTCATGAAGCAT+TGG + chr5_4:8849016-8849035 Msa0835480:CDS 25.0%
! AATGTAAATAGAGTTTCTTC+AGG - chr5_4:8849074-8849093 None:intergenic 25.0%
! AGATTTAACATCAAGTAGTT+TGG + chr5_4:8849214-8849233 Msa0835480:CDS 25.0%
! TATCCAAAGATAATTAAACG+CGG - chr5_4:8849255-8849274 None:intergenic 25.0%
!!! TTTGATTTTTGTTCATGGTA+GGG - chr5_4:8848251-8848270 None:intergenic 25.0%
!!! ATTTGATTTTTGTTCATGGT+AGG - chr5_4:8848252-8848271 None:intergenic 25.0%
! TAAAACAAGAAACGTGATTA+TGG + chr5_4:8848914-8848933 Msa0835480:CDS 25.0%
! TAATAAATCTCATGAAGCAT+TGG + chr5_4:8849016-8849035 Msa0835480:CDS 25.0%
! AATGTAAATAGAGTTTCTTC+AGG - chr5_4:8849074-8849093 None:intergenic 25.0%
! AGATTTAACATCAAGTAGTT+TGG + chr5_4:8849214-8849233 Msa0835480:CDS 25.0%
! TATCCAAAGATAATTAAACG+CGG - chr5_4:8849255-8849274 None:intergenic 25.0%
!! AGGGATTTGATTTTTGTTCA+TGG - chr5_4:8848256-8848275 None:intergenic 30.0%
AACAAGGACTAATAACTACA+GGG - chr5_4:8848275-8848294 None:intergenic 30.0%
!! ATTGTTACTTTCAATTGCAC+TGG - chr5_4:8848376-8848395 None:intergenic 30.0%
GTTTCTTCAACTTATTCAGA+CGG + chr5_4:8848948-8848967 Msa0835480:CDS 30.0%
AATCCGCGTTTAATTATCTT+TGG + chr5_4:8849249-8849268 Msa0835480:CDS 30.0%
AGAATGTGATAGCATATGAA+GGG + chr5_4:8849357-8849376 Msa0835480:CDS 30.0%
!! AGGGATTTGATTTTTGTTCA+TGG - chr5_4:8848256-8848275 None:intergenic 30.0%
AACAAGGACTAATAACTACA+GGG - chr5_4:8848275-8848294 None:intergenic 30.0%
!! ATTGTTACTTTCAATTGCAC+TGG - chr5_4:8848376-8848395 None:intergenic 30.0%
GTTTCTTCAACTTATTCAGA+CGG + chr5_4:8848948-8848967 Msa0835480:CDS 30.0%
AATCCGCGTTTAATTATCTT+TGG + chr5_4:8849249-8849268 Msa0835480:CDS 30.0%
AGAATGTGATAGCATATGAA+GGG + chr5_4:8849357-8849376 Msa0835480:CDS 30.0%
GAACAAGGACTAATAACTAC+AGG - chr5_4:8848276-8848295 None:intergenic 35.0%
AAGTGTAGTAGAGGGTTATT+AGG - chr5_4:8848312-8848331 None:intergenic 35.0%
ACTAGCACAACAAATCCTTT+GGG + chr5_4:8848401-8848420 Msa0835480:CDS 35.0%
AGAAAACTCGTGTGTTTGTA+TGG - chr5_4:8848586-8848605 None:intergenic 35.0%
! GCAATTCTAGAAGCTACTAA+AGG + chr5_4:8848675-8848694 Msa0835480:CDS 35.0%
! AACCTCATGATGATTTGCAA+TGG - chr5_4:8848769-8848788 None:intergenic 35.0%
!! TAAGAAAGATCAAGCATTGG+TGG - chr5_4:8848861-8848880 None:intergenic 35.0%
TCGTAAGAAAGATCAAGCAT+TGG - chr5_4:8848864-8848883 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG + chr5_4:8848870-8848889 Msa0835480:CDS 35.0%
CGACGAATTAGGTTCAAAAT+TGG + chr5_4:8848881-8848900 Msa0835480:CDS 35.0%
!! CGAAGTTTTTAGAAGCTGTA+CGG + chr5_4:8849150-8849169 Msa0835480:CDS 35.0%
GGTATGAAGCTGAAATTTGT+TGG + chr5_4:8849327-8849346 Msa0835480:CDS 35.0%
!! TGAAGCTGAAATTTGTTGGA+AGG + chr5_4:8849331-8849350 Msa0835480:CDS 35.0%
GAGAATGTGATAGCATATGA+AGG + chr5_4:8849356-8849375 Msa0835480:CDS 35.0%
! AGTTTGTTTCAGTGAGGATT+CGG + chr5_4:8849451-8849470 Msa0835480:CDS 35.0%
ATGTTATCTTTGCTTCTGCT+TGG + chr5_4:8849567-8849586 Msa0835480:CDS 35.0%
GAACAAGGACTAATAACTAC+AGG - chr5_4:8848276-8848295 None:intergenic 35.0%
AAGTGTAGTAGAGGGTTATT+AGG - chr5_4:8848312-8848331 None:intergenic 35.0%
ACTAGCACAACAAATCCTTT+GGG + chr5_4:8848401-8848420 Msa0835480:CDS 35.0%
AGAAAACTCGTGTGTTTGTA+TGG - chr5_4:8848586-8848605 None:intergenic 35.0%
! GCAATTCTAGAAGCTACTAA+AGG + chr5_4:8848675-8848694 Msa0835480:CDS 35.0%
! AACCTCATGATGATTTGCAA+TGG - chr5_4:8848769-8848788 None:intergenic 35.0%
!! TAAGAAAGATCAAGCATTGG+TGG - chr5_4:8848861-8848880 None:intergenic 35.0%
TCGTAAGAAAGATCAAGCAT+TGG - chr5_4:8848864-8848883 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG + chr5_4:8848870-8848889 Msa0835480:CDS 35.0%
CGACGAATTAGGTTCAAAAT+TGG + chr5_4:8848881-8848900 Msa0835480:CDS 35.0%
!! CGAAGTTTTTAGAAGCTGTA+CGG + chr5_4:8849150-8849169 Msa0835480:CDS 35.0%
GGTATGAAGCTGAAATTTGT+TGG + chr5_4:8849327-8849346 Msa0835480:CDS 35.0%
!! TGAAGCTGAAATTTGTTGGA+AGG + chr5_4:8849331-8849350 Msa0835480:CDS 35.0%
GAGAATGTGATAGCATATGA+AGG + chr5_4:8849356-8849375 Msa0835480:CDS 35.0%
! AGTTTGTTTCAGTGAGGATT+CGG + chr5_4:8849451-8849470 Msa0835480:CDS 35.0%
ATGTTATCTTTGCTTCTGCT+TGG + chr5_4:8849567-8849586 Msa0835480:CDS 35.0%
!! AAGTGTGTGGTTTGGTGAAA+AGG - chr5_4:8848222-8848241 None:intergenic 40.0%
AGTAGAGGGTTATTAGGTGT+GGG - chr5_4:8848306-8848325 None:intergenic 40.0%
TAGTAGAGGGTTATTAGGTG+TGG - chr5_4:8848307-8848326 None:intergenic 40.0%
CGTTGTTGAAGTGTAGTAGA+GGG - chr5_4:8848320-8848339 None:intergenic 40.0%
CACTAGCACAACAAATCCTT+TGG + chr5_4:8848400-8848419 Msa0835480:CDS 40.0%
! TTGTGTTGTTAAGGACCCAA+AGG - chr5_4:8848419-8848438 None:intergenic 40.0%
!! CTGAGAGTGTTGTGTTGTTA+AGG - chr5_4:8848428-8848447 None:intergenic 40.0%
!! TTCTGGAATATTGCACCTAC+AGG - chr5_4:8848517-8848536 None:intergenic 40.0%
TTAACACCTTGGCATCGTTT+CGG + chr5_4:8848633-8848652 Msa0835480:CDS 40.0%
! GCTGTTAGCTTGATTAAAGG+AGG - chr5_4:8848792-8848811 None:intergenic 40.0%
! GCAGCTGTTAGCTTGATTAA+AGG - chr5_4:8848795-8848814 None:intergenic 40.0%
CAGCTGCAGTTAATAACTGT+AGG + chr5_4:8848808-8848827 Msa0835480:CDS 40.0%
! GCATTGGTGGAATTGGAATA+TGG - chr5_4:8848848-8848867 None:intergenic 40.0%
!! AGATCAAGCATTGGTGGAAT+TGG - chr5_4:8848855-8848874 None:intergenic 40.0%
AGATCCAACAATAGTCGTCT+TGG + chr5_4:8849178-8849197 Msa0835480:CDS 40.0%
TCTTTGGATACCGTTTGACA+CGG + chr5_4:8849265-8849284 Msa0835480:CDS 40.0%
! TGGACACATTTTTACCACGA+GGG + chr5_4:8849288-8849307 Msa0835480:CDS 40.0%
! AGTCGAGTCTAAAACCAAGT+GGG + chr5_4:8849397-8849416 Msa0835480:CDS 40.0%
!! ATGAGAAAGGCACGTTTTCA+AGG + chr5_4:8849428-8849447 Msa0835480:CDS 40.0%
! TCAAGGAGTTTGTTTCAGTG+AGG + chr5_4:8849445-8849464 Msa0835480:CDS 40.0%
!! TTTCAGTGAGGATTCGGTTT+TGG + chr5_4:8849457-8849476 Msa0835480:CDS 40.0%
!!! GATTCGGTTTTGGAGGTTAA+GGG + chr5_4:8849467-8849486 Msa0835480:CDS 40.0%
ATGAGCTTCTTGTGCTTACT+TGG + chr5_4:8849534-8849553 Msa0835480:CDS 40.0%
CTTCTTGTGCTTACTTGGAA+AGG + chr5_4:8849539-8849558 Msa0835480:CDS 40.0%
!! AAGTGTGTGGTTTGGTGAAA+AGG - chr5_4:8848222-8848241 None:intergenic 40.0%
AGTAGAGGGTTATTAGGTGT+GGG - chr5_4:8848306-8848325 None:intergenic 40.0%
TAGTAGAGGGTTATTAGGTG+TGG - chr5_4:8848307-8848326 None:intergenic 40.0%
CGTTGTTGAAGTGTAGTAGA+GGG - chr5_4:8848320-8848339 None:intergenic 40.0%
CACTAGCACAACAAATCCTT+TGG + chr5_4:8848400-8848419 Msa0835480:CDS 40.0%
! TTGTGTTGTTAAGGACCCAA+AGG - chr5_4:8848419-8848438 None:intergenic 40.0%
!! CTGAGAGTGTTGTGTTGTTA+AGG - chr5_4:8848428-8848447 None:intergenic 40.0%
!! TTCTGGAATATTGCACCTAC+AGG - chr5_4:8848517-8848536 None:intergenic 40.0%
TTAACACCTTGGCATCGTTT+CGG + chr5_4:8848633-8848652 Msa0835480:CDS 40.0%
! GCTGTTAGCTTGATTAAAGG+AGG - chr5_4:8848792-8848811 None:intergenic 40.0%
! GCAGCTGTTAGCTTGATTAA+AGG - chr5_4:8848795-8848814 None:intergenic 40.0%
CAGCTGCAGTTAATAACTGT+AGG + chr5_4:8848808-8848827 Msa0835480:CDS 40.0%
! GCATTGGTGGAATTGGAATA+TGG - chr5_4:8848848-8848867 None:intergenic 40.0%
!! AGATCAAGCATTGGTGGAAT+TGG - chr5_4:8848855-8848874 None:intergenic 40.0%
AGATCCAACAATAGTCGTCT+TGG + chr5_4:8849178-8849197 Msa0835480:CDS 40.0%
TCTTTGGATACCGTTTGACA+CGG + chr5_4:8849265-8849284 Msa0835480:CDS 40.0%
! TGGACACATTTTTACCACGA+GGG + chr5_4:8849288-8849307 Msa0835480:CDS 40.0%
! AGTCGAGTCTAAAACCAAGT+GGG + chr5_4:8849397-8849416 Msa0835480:CDS 40.0%
!! ATGAGAAAGGCACGTTTTCA+AGG + chr5_4:8849428-8849447 Msa0835480:CDS 40.0%
! TCAAGGAGTTTGTTTCAGTG+AGG + chr5_4:8849445-8849464 Msa0835480:CDS 40.0%
!! TTTCAGTGAGGATTCGGTTT+TGG + chr5_4:8849457-8849476 Msa0835480:CDS 40.0%
!!! GATTCGGTTTTGGAGGTTAA+GGG + chr5_4:8849467-8849486 Msa0835480:CDS 40.0%
ATGAGCTTCTTGTGCTTACT+TGG + chr5_4:8849534-8849553 Msa0835480:CDS 40.0%
CTTCTTGTGCTTACTTGGAA+AGG + chr5_4:8849539-8849558 Msa0835480:CDS 40.0%
!! TGTGTGGTTTGGTGAAAAGG+TGG - chr5_4:8848219-8848238 None:intergenic 45.0%
! GGTTGCAAAAGTGTGTGGTT+TGG - chr5_4:8848230-8848249 None:intergenic 45.0%
GGTGTGGGAAATTCTGAACA+AGG - chr5_4:8848291-8848310 None:intergenic 45.0%
GCGTTGTTGAAGTGTAGTAG+AGG - chr5_4:8848321-8848340 None:intergenic 45.0%
CTTCAACAACGCCAATTGCA+TGG + chr5_4:8848329-8848348 Msa0835480:CDS 45.0%
GAGTAACTGCTCCATGCAAT+TGG - chr5_4:8848343-8848362 None:intergenic 45.0%
CGCCATTGCAAATCATCATG+AGG + chr5_4:8848764-8848783 Msa0835480:CDS 45.0%
AATGTTGCACTTGCACTCGT+AGG - chr5_4:8848995-8849014 None:intergenic 45.0%
TCCAACAATAGTCGTCTTGG+TGG + chr5_4:8849181-8849200 Msa0835480:CDS 45.0%
TCCACCAAGACGACTATTGT+TGG - chr5_4:8849185-8849204 None:intergenic 45.0%
AATGTGTCCACCGTGTCAAA+CGG - chr5_4:8849278-8849297 None:intergenic 45.0%
! GTGGACACATTTTTACCACG+AGG + chr5_4:8849287-8849306 Msa0835480:CDS 45.0%
TACCACGAGGGAGTAAACAA+AGG + chr5_4:8849300-8849319 Msa0835480:CDS 45.0%
! GAGTCGAGTCTAAAACCAAG+TGG + chr5_4:8849396-8849415 Msa0835480:CDS 45.0%
!!! GGATTCGGTTTTGGAGGTTA+AGG + chr5_4:8849466-8849485 Msa0835480:CDS 45.0%
ATGCTTGATGTACATGCAGC+TGG + chr5_4:8849491-8849510 Msa0835480:CDS 45.0%
TTGATGTACATGCAGCTGGA+TGG + chr5_4:8849495-8849514 Msa0835480:CDS 45.0%
TGATGTACATGCAGCTGGAT+GGG + chr5_4:8849496-8849515 Msa0835480:CDS 45.0%
!! TGTGTGGTTTGGTGAAAAGG+TGG - chr5_4:8848219-8848238 None:intergenic 45.0%
! GGTTGCAAAAGTGTGTGGTT+TGG - chr5_4:8848230-8848249 None:intergenic 45.0%
GGTGTGGGAAATTCTGAACA+AGG - chr5_4:8848291-8848310 None:intergenic 45.0%
GCGTTGTTGAAGTGTAGTAG+AGG - chr5_4:8848321-8848340 None:intergenic 45.0%
CTTCAACAACGCCAATTGCA+TGG + chr5_4:8848329-8848348 Msa0835480:CDS 45.0%
GAGTAACTGCTCCATGCAAT+TGG - chr5_4:8848343-8848362 None:intergenic 45.0%
CGCCATTGCAAATCATCATG+AGG + chr5_4:8848764-8848783 Msa0835480:CDS 45.0%
AATGTTGCACTTGCACTCGT+AGG - chr5_4:8848995-8849014 None:intergenic 45.0%
TCCAACAATAGTCGTCTTGG+TGG + chr5_4:8849181-8849200 Msa0835480:CDS 45.0%
TCCACCAAGACGACTATTGT+TGG - chr5_4:8849185-8849204 None:intergenic 45.0%
AATGTGTCCACCGTGTCAAA+CGG - chr5_4:8849278-8849297 None:intergenic 45.0%
! GTGGACACATTTTTACCACG+AGG + chr5_4:8849287-8849306 Msa0835480:CDS 45.0%
TACCACGAGGGAGTAAACAA+AGG + chr5_4:8849300-8849319 Msa0835480:CDS 45.0%
! GAGTCGAGTCTAAAACCAAG+TGG + chr5_4:8849396-8849415 Msa0835480:CDS 45.0%
!!! GGATTCGGTTTTGGAGGTTA+AGG + chr5_4:8849466-8849485 Msa0835480:CDS 45.0%
ATGCTTGATGTACATGCAGC+TGG + chr5_4:8849491-8849510 Msa0835480:CDS 45.0%
TTGATGTACATGCAGCTGGA+TGG + chr5_4:8849495-8849514 Msa0835480:CDS 45.0%
TGATGTACATGCAGCTGGAT+GGG + chr5_4:8849496-8849515 Msa0835480:CDS 45.0%
! GGTAGGGTTGCAAAAGTGTG+TGG - chr5_4:8848235-8848254 None:intergenic 50.0%
CTCGAATGAAGCTTCCTGCT+AGG - chr5_4:8848470-8848489 None:intergenic 50.0%
GCGGTTTGTGTAACTGCTTC+TGG - chr5_4:8848534-8848553 None:intergenic 50.0%
GTGTAACCGAAACGATGCCA+AGG - chr5_4:8848642-8848661 None:intergenic 50.0%
!! GCAATGGCGTCGATTAGTGT+AGG - chr5_4:8848753-8848772 None:intergenic 50.0%
TTGGATACCGTTTGACACGG+TGG + chr5_4:8849268-8849287 Msa0835480:CDS 50.0%
! TGCCTTTGTTTACTCCCTCG+TGG - chr5_4:8849305-8849324 None:intergenic 50.0%
GAGGGAGTAAACAAAGGCAG+TGG + chr5_4:8849306-8849325 Msa0835480:CDS 50.0%
TCTCATTCGTTGCTCCCACT+TGG - chr5_4:8849414-8849433 None:intergenic 50.0%
GTGGGAGCAACGAATGAGAA+AGG + chr5_4:8849415-8849434 Msa0835480:CDS 50.0%
!! CAGTGAGGATTCGGTTTTGG+AGG + chr5_4:8849460-8849479 Msa0835480:CDS 50.0%
GATGTACATGCAGCTGGATG+GGG + chr5_4:8849497-8849516 Msa0835480:CDS 50.0%
! GGTAGGGTTGCAAAAGTGTG+TGG - chr5_4:8848235-8848254 None:intergenic 50.0%
CTCGAATGAAGCTTCCTGCT+AGG - chr5_4:8848470-8848489 None:intergenic 50.0%
GCGGTTTGTGTAACTGCTTC+TGG - chr5_4:8848534-8848553 None:intergenic 50.0%
GTGTAACCGAAACGATGCCA+AGG - chr5_4:8848642-8848661 None:intergenic 50.0%
!! GCAATGGCGTCGATTAGTGT+AGG - chr5_4:8848753-8848772 None:intergenic 50.0%
TTGGATACCGTTTGACACGG+TGG + chr5_4:8849268-8849287 Msa0835480:CDS 50.0%
! TGCCTTTGTTTACTCCCTCG+TGG - chr5_4:8849305-8849324 None:intergenic 50.0%
GAGGGAGTAAACAAAGGCAG+TGG + chr5_4:8849306-8849325 Msa0835480:CDS 50.0%
TCTCATTCGTTGCTCCCACT+TGG - chr5_4:8849414-8849433 None:intergenic 50.0%
GTGGGAGCAACGAATGAGAA+AGG + chr5_4:8849415-8849434 Msa0835480:CDS 50.0%
!! CAGTGAGGATTCGGTTTTGG+AGG + chr5_4:8849460-8849479 Msa0835480:CDS 50.0%
GATGTACATGCAGCTGGATG+GGG + chr5_4:8849497-8849516 Msa0835480:CDS 50.0%
GTCTCCAATCAACGCCTAGC+AGG + chr5_4:8848453-8848472 Msa0835480:CDS 55.0%
!! GCTTCCTGCTAGGCGTTGAT+TGG - chr5_4:8848460-8848479 None:intergenic 55.0%
CGCGTGCAAGAAGATCCTGT+AGG + chr5_4:8848499-8848518 Msa0835480:CDS 55.0%
GTCTCCAATCAACGCCTAGC+AGG + chr5_4:8848453-8848472 Msa0835480:CDS 55.0%
!! GCTTCCTGCTAGGCGTTGAT+TGG - chr5_4:8848460-8848479 None:intergenic 55.0%
CGCGTGCAAGAAGATCCTGT+AGG + chr5_4:8848499-8848518 Msa0835480:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 8848192 8849601 8848192 ID=Msa0835480;Name=Msa0835480
chr5_4 mRNA 8848192 8849601 8848192 ID=Msa0835480-mRNA-1;Parent=Msa0835480;Name=Msa0835480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|469
chr5_4 exon 8848192 8849601 8848192 ID=Msa0835480-mRNA-1:exon:3874;Parent=Msa0835480-mRNA-1
chr5_4 CDS 8848192 8849601 8848192 ID=Msa0835480-mRNA-1:cds;Parent=Msa0835480-mRNA-1
Gene Sequence

>Msa0835480

ATGACGCAATTCACATCAACATTTCCACCTTTTCACCAAACCACACACTTTTGCAACCCTACCATGAACAAAAATCAAATCCCTGTAGTTATTAGTCCTTGTTCAGAATTTCCCACACCTAATAACCCTCTACTACACTTCAACAACGCCAATTGCATGGAGCAGTTACTCATTCACTGTGCCAGTGCAATTGAAAGTAACAATGCAACACTAGCACAACAAATCCTTTGGGTCCTTAACAACACAACACTCTCAGACGACGTCTCCAATCAACGCCTAGCAGGAAGCTTCATTCGAGCTCTTACAACGCGTGCAAGAAGATCCTGTAGGTGCAATATTCCAGAAGCAGTTACACAAACCGCAAATAGTTATAACTATCATCACAATCTCGCCATACAAACACACGAGTTTTCTGTCATTGAGTTAGCAAATTTTATTGATTTAACACCTTGGCATCGTTTCGGTTACACAGCAGCTAATTCTGCAATTCTAGAAGCTACTAAAGGATTTTCAGTTATTCACATTGTTGATTTGAGTTTAACACACTGCATGCAGATTCCTACACTAATCGACGCCATTGCAAATCATCATGAGGTTCCTCCTTTAATCAAGCTAACAGCTGCAGTTAATAACTGTAGGAATAAGAATATTAACCATATTCCAATTCCACCAATGCTTGATCTTTCTTACGACGAATTAGGTTCAAAATTGGTTAATTTTGCTAAAACAAGAAACGTGATTATGGAATTCAAAGCTGTTTCTTCAACTTATTCAGACGGATTTGCTTCGTTGATTGAACACCTACGAGTGCAAGTGCAACATTTTAATAAATCTCATGAAGCATTGGTTATAAACTGTCACATGATGCTTCATTATATTCCTGAAGAAACTCTATTTACATTTAAAGATACAAATTCAAATTCAAATTTGAATTTAAACTTTTGTTCATCGCTGCGCTCGAAGTTTTTAGAAGCTGTACGGAGTTTAGATCCAACAATAGTCGTCTTGGTGGATGAAGATGCAGATTTAACATCAAGTAGTTTGGTTAGTAGACTAAAATCCGCGTTTAATTATCTTTGGATACCGTTTGACACGGTGGACACATTTTTACCACGAGGGAGTAAACAAAGGCAGTGGTATGAAGCTGAAATTTGTTGGAAGGTTGAGAATGTGATAGCATATGAAGGGATTCAGAGAGTTGAGAGAGTCGAGTCTAAAACCAAGTGGGAGCAACGAATGAGAAAGGCACGTTTTCAAGGAGTTTGTTTCAGTGAGGATTCGGTTTTGGAGGTTAAGGGTATGCTTGATGTACATGCAGCTGGATGGGGATTGAAGAAAGAAGATGAGCTTCTTGTGCTTACTTGGAAAGGACATAATGTTATCTTTGCTTCTGCTTGGTTACCTTCTTGA
Protein sequence

>Msa0835480

MTQFTSTFPPFHQTTHFCNPTMNKNQIPVVISPCSEFPTPNNPLLHFNNANCMEQLLIHCASAIESNNATLAQQILWVLNNTTLSDDVSNQRLAGSFIRALTTRARRSCRCNIPEAVTQTANSYNYHHNLAIQTHEFSVIELANFIDLTPWHRFGYTAANSAILEATKGFSVIHIVDLSLTHCMQIPTLIDAIANHHEVPPLIKLTAAVNNCRNKNINHIPIPPMLDLSYDELGSKLVNFAKTRNVIMEFKAVSSTYSDGFASLIEHLRVQVQHFNKSHEALVINCHMMLHYIPEETLFTFKDTNSNSNLNLNFCSSLRSKFLEAVRSLDPTIVVLVDEDADLTSSSLVSRLKSAFNYLWIPFDTVDTFLPRGSKQRQWYEAEICWKVENVIAYEGIQRVERVESKTKWEQRMRKARFQGVCFSEDSVLEVKGMLDVHAAGWGLKKEDELLVLTWKGHNVIFASAWLPS*