AlfalfaGEDB | Msa0837150

M. sativa cultivar zhongmu-4 / Msa0837150

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0837150	XP_039685680.1	84.731	799	102	3	1	779	1	799	0.0	1387

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0837150	sp\|Q9SZL8\|FRS5_ARATH	27.769	641	383	14	32	665	70	637	2.32e-68	244

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0837150	D3YBB9	76.208	807	175	4	1	798	49	847	0.0	1277

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TFs/TRs:

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Gene ID	Type	Classification
Msa0837150	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0837150	MtrunA17_Chr3g0088371	82.609	184	29	1	618	798	1	184	4.28e-102	313
Msa0837150	MtrunA17_Chr2g0324011	30.551	635	365	12	36	665	8	571	5.92e-100	322
Msa0837150	MtrunA17_Chr3g0082121	31.548	691	385	17	41	727	49	655	2.06e-99	323
Msa0837150	MtrunA17_Chr1g0202341	29.449	635	367	17	34	662	50	609	1.17e-78	267
Msa0837150	MtrunA17_Chr8g0363841	28.617	629	376	13	40	665	22	580	1.01e-73	253
Msa0837150	MtrunA17_Chr1g0203721	25.897	780	453	20	1	739	16	711	1.12e-71	252
Msa0837150	MtrunA17_Chr5g0448761	25.177	707	450	14	31	728	56	692	3.50e-67	238
Msa0837150	MtrunA17_Chr2g0296781	27.541	679	406	20	39	708	1	602	4.41e-67	236
Msa0837150	MtrunA17_Chr6g0458391	26.984	630	357	15	41	662	231	765	6.24e-65	231
Msa0837150	MtrunA17_Chr6g0477261	29.222	527	315	14	180	699	7	482	1.17e-63	225

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0837150	AT4G38180.1	27.769	641	383	14	32	665	70	637	2.35e-69	244
Msa0837150	AT4G15090.1	25.000	696	435	15	50	741	66	678	5.38e-59	216
Msa0837150	AT4G15090.5	25.000	696	435	15	50	741	66	678	5.38e-59	216
Msa0837150	AT4G15090.3	25.000	696	435	15	50	741	66	678	5.38e-59	216
Msa0837150	AT4G15090.2	25.000	696	435	15	50	741	66	678	5.38e-59	216
Msa0837150	AT4G15090.4	25.000	696	435	15	50	741	66	678	5.38e-59	216
Msa0837150	AT2G27110.2	24.930	714	424	19	10	719	29	634	1.73e-52	197
Msa0837150	AT2G27110.1	24.930	714	424	19	10	719	29	634	1.73e-52	197
Msa0837150	AT2G27110.3	27.112	509	314	11	213	719	36	489	7.13e-52	193
Msa0837150	AT3G06250.2	24.961	633	352	16	34	661	189	703	5.52e-51	191

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Find 122 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTCTGGTTTATGTAA+TGG	0.504865	5_4:-10953496	None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG	0.545537	5_4:+10953298	Msa0837150:CDS
AACAATAAATATCGGCCATT+AGG	0.388497	5_4:+10953898	Msa0837150:CDS
AACTCGTCAAAAGGAGATGG+TGG	0.603142	5_4:+10953690	Msa0837150:CDS
AAGAAAGACCAAATCCAATT+AGG	0.397771	5_4:+10955746	Msa0837150:CDS
AATCAGATTTCGACTACAAT+TGG	0.318540	5_4:+10954666	Msa0837150:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	5_4:-10954869	None:intergenic
AATGAAGCTCTCTCGAAACC+AGG	0.565261	5_4:+10953748	Msa0837150:CDS
AATTCTTGATTGTTATGCAT+TGG	0.467731	5_4:+10955984	Msa0837150:three_prime_UTR
ACAAAATAAGAAATCGGATA+AGG	0.469178	5_4:+10953252	Msa0837150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATTAACTTCATCTGAGAG+TGG	+	chr5_4:10953046-10953065	Msa0837150:CDS	30.0%
TAAAGAATCATGGAAGTCTT+TGG	+	chr5_4:10953132-10953151	Msa0837150:CDS	30.0%
CGTTATGTTTGTAACAAAGA+AGG	+	chr5_4:10953286-10953305	Msa0837150:CDS	30.0%
TAACAAAGAAGGTTTCAAGA+AGG	+	chr5_4:10953297-10953316	Msa0837150:CDS	30.0%
AACAAAGAAGGTTTCAAGAA+GGG	+	chr5_4:10953298-10953317	Msa0837150:CDS	30.0%
TAAAGAAAAACCAGAGGAAA+GGG	+	chr5_4:10953387-10953406	Msa0837150:CDS	30.0%
AGAACATCTTATGAAGTGAT+GGG	+	chr5_4:10953601-10953620	Msa0837150:CDS	30.0%
TTTAAGTCCGAAAATCGAAA+AGG	-	chr5_4:10953655-10953674	None:intergenic	30.0%
TGGATATCATAGTAAAAACC+TGG	-	chr5_4:10953769-10953788	None:intergenic	30.0%
GCCGATATTTATTGTTTGTT+CGG	-	chr5_4:10953894-10953913	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	10952992	10956025	10952992	ID=Msa0837150;Name=Msa0837150
chr5_4	mRNA	10952992	10956025	10952992	ID=Msa0837150-mRNA-1;Parent=Msa0837150;Name=Msa0837150-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45\|1\|1\|1\|1\|1\|2\|152\|800
chr5_4	exon	10952992	10953919	10952992	ID=Msa0837150-mRNA-1:exon:4758;Parent=Msa0837150-mRNA-1
chr5_4	exon	10954354	10956025	10954354	ID=Msa0837150-mRNA-1:exon:4759;Parent=Msa0837150-mRNA-1
chr5_4	five_prime_UTR	10952992	10953036	10952992	ID=Msa0837150-mRNA-1:five_prime_utr;Parent=Msa0837150-mRNA-1
chr5_4	CDS	10953037	10953919	10953037	ID=Msa0837150-mRNA-1:cds;Parent=Msa0837150-mRNA-1
chr5_4	CDS	10954354	10955873	10954354	ID=Msa0837150-mRNA-1:cds;Parent=Msa0837150-mRNA-1
chr5_4	three_prime_UTR	10955874	10956025	10955874	ID=Msa0837150-mRNA-1:three_prime_utr;Parent=Msa0837150-mRNA-1

Gene Sequence

>Msa0837150

ATGGATTTGATATTAACTTCATCTGAGAGTGGACAATTGACGGTTGAAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCATTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGTTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAAGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGTGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCCAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTTGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCGGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACACAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAGTTGGACTTTAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCAGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAATTTGAAGAAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTGGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCCACCATGAATGTTGGCACTAAAGACACCACTGAAGCGCAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTTTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTGTGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAGACAGAAATTCCTTATAGCTCAGAAGAAGCAAAAGAAAGATGATGAGGCTTTAACAAATTAA

Protein sequence

>Msa0837150

MDLILTSSESGQLTVENSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYVCNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYKWEVDHCHPLHKPEHLCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFCDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHAELDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAEFEENLSPRFEDHNRLDERVVTVDIGSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTEAQRYQQICAVTVQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVCEPSVGTAPKFKERPNPIRSKKRLKSDYEKARARQKFLIAQKKQKKDDEALTN*