AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0839400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0839400 MtrunA17_Chr2g0296781 72.727 737 189 5 37 771 1 727 0.0 1092
Msa0839400 MtrunA17_Chr6g0477261 72.939 643 149 4 149 768 1 641 0.0 947
Msa0839400 MtrunA17_Chr1g0196541 62.010 766 256 4 37 802 1 731 0.0 947
Msa0839400 MtrunA17_Chr5g0441581 48.226 620 304 6 27 640 21 629 0.0 584
Msa0839400 MtrunA17_Chr2g0291581 37.797 590 347 9 11 594 47 622 2.19e-132 409
Msa0839400 MtrunA17_Chr1g0207551 36.861 567 347 5 30 593 31 589 2.47e-126 397
Msa0839400 MtrunA17_Chr5g0448761 37.629 582 338 11 27 593 54 625 1.86e-124 392
Msa0839400 MtrunA17_Chr2g0329031 36.667 570 336 10 25 588 6 556 3.48e-122 384
Msa0839400 MtrunA17_Chr1g0202341 37.805 574 333 11 32 594 50 610 3.78e-122 383
Msa0839400 MtrunA17_Chr3g0080351 35.726 585 327 9 27 593 70 623 1.97e-117 375
Msa0839400 MtrunA17_Chr2g0291591 34.725 527 338 4 2 525 33 556 8.03e-108 342
Msa0839400 MtrunA17_Chr5g0440451 33.008 615 359 15 32 619 42 630 1.25e-94 316
Msa0839400 MtrunA17_Chr5g0448511 36.946 406 244 5 191 593 1 397 4.21e-88 289
Msa0839400 MtrunA17_Chr2g0287651 28.595 619 423 9 37 647 79 686 6.06e-87 294
Msa0839400 MtrunA17_Chr3g0118611 38.776 392 231 4 203 593 118 501 3.84e-86 290
Msa0839400 MtrunA17_Chr2g0324011 29.879 579 374 10 30 593 7 568 1.74e-82 275
Msa0839400 MtrunA17_Chr8g0363841 30.705 596 379 13 29 605 10 590 7.92e-82 276
Msa0839400 MtrunA17_Chr2g0287061 32.509 566 328 15 32 591 182 699 9.47e-80 273
Msa0839400 MtrunA17_Chr6g0476441 52.941 289 92 3 533 778 1 288 3.48e-78 254
Msa0839400 MtrunA17_Chr2g0276841 29.611 591 369 15 23 590 60 626 1.07e-75 259
Msa0839400 MtrunA17_Chr1g0198761 28.571 707 421 23 36 700 50 714 1.81e-73 254
Msa0839400 MtrunA17_Chr6g0458391 28.399 581 379 12 22 593 213 765 5.58e-71 248
Msa0839400 MtrunA17_Chr1g0203721 29.145 573 378 10 35 593 65 623 3.96e-69 245
Msa0839400 MtrunA17_Chr1g0203051 27.731 595 382 14 7 574 43 616 9.05e-69 239
Msa0839400 MtrunA17_Chr3g0082121 30.298 604 369 18 12 593 17 590 8.43e-66 232
Msa0839400 MtrunA17_Chr2g0294841 26.783 575 388 11 33 589 45 604 7.98e-62 222
Msa0839400 MtrunA17_Chr2g0290391 26.264 613 394 16 9 591 27 611 7.81e-53 195
Msa0839400 MtrunA17_Chr5g0431001 33.019 318 202 5 126 437 168 480 2.55e-52 190
Msa0839400 MtrunA17_Chr1g0164591 33.016 315 187 7 115 421 10 308 2.03e-47 171
Msa0839400 MtrunA17_Chr7g0258861 33.101 287 186 3 26 306 3 289 7.57e-47 169
Msa0839400 MtrunA17_Chr6g0476431 59.302 86 35 0 37 122 1 86 2.99e-30 114
Msa0839400 MtrunA17_Chr6g0451441 30.851 188 122 4 13 195 63 247 3.27e-20 94.7
Msa0839400 MtrunA17_Chr2g0308841 25.108 231 158 3 365 590 4 224 1.72e-19 89.4
Msa0839400 MtrunA17_Chr3g0077731 56.364 110 45 3 646 754 1 108 1.66e-18 82.0
Msa0839400 MtrunA17_Chr1g0172041 33.636 110 72 1 505 614 1 109 5.07e-13 66.2
Msa0839400 MtrunA17_Chr2g0329361 30.400 125 71 3 32 145 60 179 3.16e-12 67.4
Msa0839400 MtrunA17_Chr1g0149131 30.000 110 76 1 308 416 1 110 4.31e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0839400 AT4G15090.1 34.039 661 418 9 15 668 33 682 2.43e-129 406
Msa0839400 AT4G15090.5 34.039 661 418 9 15 668 33 682 2.43e-129 406
Msa0839400 AT4G15090.3 34.039 661 418 9 15 668 33 682 2.43e-129 406
Msa0839400 AT4G15090.2 34.039 661 418 9 15 668 33 682 2.43e-129 406
Msa0839400 AT4G15090.4 34.039 661 418 9 15 668 33 682 2.43e-129 406
Msa0839400 AT1G52520.1 35.126 595 359 11 29 613 81 658 3.55e-119 376
Msa0839400 AT4G38180.1 35.052 582 363 8 20 593 60 634 2.07e-118 376
Msa0839400 AT3G22170.2 36.007 586 327 10 26 593 63 618 4.25e-116 372
Msa0839400 AT3G22170.1 36.007 586 327 10 26 593 63 618 4.25e-116 372
Msa0839400 AT1G76320.3 36.111 576 339 10 37 601 1 558 1.56e-113 360
Msa0839400 AT1G76320.1 36.111 576 339 10 37 601 1 558 2.23e-113 362
Msa0839400 AT1G76320.4 36.111 576 339 10 37 601 1 558 2.23e-113 362
Msa0839400 AT1G76320.2 36.111 576 339 10 37 601 1 558 2.40e-113 361
Msa0839400 AT1G80010.1 34.775 601 356 16 20 597 78 665 3.58e-107 345
Msa0839400 AT2G27110.2 32.215 596 368 8 8 598 20 584 6.04e-93 310
Msa0839400 AT2G27110.1 32.215 596 368 8 8 598 20 584 6.04e-93 310
Msa0839400 AT4G19990.3 32.767 589 312 10 36 593 24 559 3.69e-92 306
Msa0839400 AT4G19990.4 32.767 589 312 10 36 593 24 559 3.69e-92 306
Msa0839400 AT4G19990.5 32.767 589 312 10 36 593 24 559 6.05e-92 305
Msa0839400 AT4G19990.7 32.767 589 312 10 36 593 38 573 8.54e-92 305
Msa0839400 AT4G19990.6 32.767 589 312 10 36 593 24 559 9.72e-92 305
Msa0839400 AT4G19990.2 32.767 589 312 10 36 593 24 559 9.72e-92 305
Msa0839400 AT2G32250.5 32.918 562 326 8 32 590 37 550 2.01e-91 304
Msa0839400 AT2G32250.6 32.918 562 326 8 32 590 37 550 4.53e-91 304
Msa0839400 AT2G32250.1 32.918 562 326 8 32 590 37 550 4.53e-91 304
Msa0839400 AT2G32250.3 32.918 562 326 8 32 590 37 550 4.53e-91 304
Msa0839400 AT2G32250.2 32.918 562 326 8 32 590 37 550 4.82e-91 304
Msa0839400 AT2G32250.4 32.918 562 326 8 32 590 37 550 4.82e-91 304
Msa0839400 AT2G32250.8 32.855 557 323 8 37 590 1 509 2.13e-90 301
Msa0839400 AT2G32250.7 32.855 557 323 8 37 590 1 509 2.22e-90 301
Msa0839400 AT4G19990.1 34.082 534 296 9 63 593 22 502 3.21e-90 299
Msa0839400 AT2G27110.3 34.389 442 273 5 158 598 14 439 1.85e-82 278
Msa0839400 AT3G06250.2 30.556 576 340 12 23 591 180 702 2.68e-80 274
Msa0839400 AT3G06250.1 30.556 576 340 12 23 591 180 702 2.68e-80 274
Msa0839400 AT3G06250.3 30.556 576 340 12 23 591 180 702 2.68e-80 274
Msa0839400 AT5G18960.2 31.393 567 332 17 32 591 210 726 2.84e-77 266
Msa0839400 AT5G18960.1 31.393 567 332 17 32 591 210 726 2.84e-77 266
Msa0839400 AT4G38170.1 34.783 391 243 5 205 593 19 399 1.29e-75 256
Msa0839400 AT1G10240.2 28.595 619 376 19 5 590 19 604 1.29e-68 240
Msa0839400 AT1G10240.1 28.595 619 376 19 5 590 19 604 1.29e-68 240
Msa0839400 AT5G28530.3 25.617 648 416 19 33 645 55 671 2.56e-53 197
Msa0839400 AT5G28530.2 25.617 648 416 19 33 645 55 671 2.56e-53 197
Msa0839400 AT5G28530.1 25.613 652 415 20 33 645 55 675 1.65e-52 195
Msa0839400 AT5G28530.5 26.913 379 246 12 276 645 252 608 1.39e-29 125
Msa0839400 AT5G28530.4 26.893 383 245 13 276 645 252 612 9.14e-29 122

Find 102 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGTTTATTTGAGTTTA+AGG 0.126982 5_4:+14331432 Msa0839400:CDS
ATTGCTTGTATAAGTCATTT+TGG 0.199496 5_4:+14331193 Msa0839400:CDS
TCCATCTTGGTCAGTAATTA+TGG 0.221359 5_4:-14330720 None:intergenic
AAGAATTATGCAAGATGTTT+AGG 0.244212 5_4:+14329924 Msa0839400:CDS
TATGCAAGATGTTTAGGTTT+TGG 0.256539 5_4:+14329930 Msa0839400:CDS
TTTGAGCATAAGTGATTTCA+TGG 0.262311 5_4:+14330892 Msa0839400:CDS
TGTATGTGAGAGACACATTT+TGG 0.270477 5_4:+14331005 Msa0839400:CDS
CACCATCGATGGCGAGCTTT+CGG 0.279320 5_4:-14330773 None:intergenic
ACACTTATTAAAAGTGGTTT+TGG 0.290751 5_4:+14331565 Msa0839400:CDS
TGGAACAATTACTATTTCTA+AGG 0.295449 5_4:+14330118 Msa0839400:CDS
TAAAATTGAAAAGGAAAATA+TGG 0.301034 5_4:+14331144 Msa0839400:CDS
TATACAAGCAATAATTCTAT+TGG 0.306437 5_4:-14331182 None:intergenic
TAGTATCCTCCTTCGACAAT+AGG 0.312589 5_4:-14330637 None:intergenic
AGGGCTGCTTATGAGTATTT+TGG 0.315150 5_4:+14330506 Msa0839400:CDS
TGAAGAAGCAAATCCGTAAA+AGG 0.325895 5_4:-14331638 None:intergenic
TGCTGTTTATTTGAGTTTAA+GGG 0.334665 5_4:+14331433 Msa0839400:CDS
CTAAAATATCTTGATTTGCT+TGG 0.342688 5_4:-14330173 None:intergenic
CATTCTTCATTGCTCCATCT+TGG 0.348218 5_4:-14330733 None:intergenic
ATATAGACCCTAGTATAAAA+AGG 0.351811 5_4:+14330207 Msa0839400:CDS
ACGCTGCAACTCTTCATTTC+CGG 0.353530 5_4:-14331596 None:intergenic
TTTCTTGGTTGTTCACTACT+TGG 0.357896 5_4:+14330666 Msa0839400:CDS
TCATGCTTTCTTTGAGAGAA+TGG 0.375982 5_4:+14331297 Msa0839400:CDS
ACCATCGATGGCGAGCTTTC+GGG 0.381382 5_4:-14330772 None:intergenic
AAAATATCTTGATGTTAATC+AGG 0.381504 5_4:+14332151 Msa0839400:CDS
TTGTGTTACAGAAAGTAAAA+AGG 0.383201 5_4:+14331339 Msa0839400:CDS
CATGGTCAGTCAGTTCTGTT+AGG 0.383640 5_4:+14330608 Msa0839400:CDS
CTTGTGGCATATAATGAAAA+AGG 0.386165 5_4:+14330796 Msa0839400:CDS
TAGAATAGAGAACTTAAAAC+TGG 0.390920 5_4:+14331699 Msa0839400:CDS
AGTATCCTCCTTCGACAATA+GGG 0.392582 5_4:-14330636 None:intergenic
ATGATGAATGATCATTTATA+TGG 0.403678 5_4:-14332409 None:intergenic
GGATATGATAACCTTACATT+CGG 0.409109 5_4:+14330302 Msa0839400:CDS
CCTTTACTTCGAGTCGCTAC+AGG 0.409218 5_4:-14331802 None:intergenic
AAGGGCGTCCTCCTTCAAAA+AGG 0.415773 5_4:+14331821 Msa0839400:CDS
TTAAATTTCATGCCAACCTT+AGG 0.424975 5_4:-14329873 None:intergenic
GTTTGATGAGCGTCATCGTT+GGG 0.431278 5_4:+14330976 Msa0839400:CDS
AAGTGAGGCGGCTTCGACTT+GGG 0.432744 5_4:+14330354 Msa0839400:CDS
ATATTTATTTGTTAAGTAAG+TGG 0.436838 5_4:-14330549 None:intergenic
TTAATATCCTTCCTCCATCT+TGG 0.438245 5_4:-14331532 None:intergenic
CTTGTTATATATTGCCAAGA+TGG 0.439590 5_4:+14331518 Msa0839400:CDS
AAGAAGCTTGCTGGAAAGAA+AGG 0.445180 5_4:+14331874 Msa0839400:CDS
CATGATAATGAATGGTTGAA+AGG 0.452805 5_4:+14330950 Msa0839400:CDS
GATATGATAACCTTACATTC+GGG 0.457280 5_4:+14330303 Msa0839400:CDS
ATTTGAATCTCAATTTCAAA+AGG 0.459389 5_4:+14331216 Msa0839400:CDS
ATGATCATTCATCATCATGA+TGG 0.461994 5_4:+14332417 Msa0839400:CDS
TCTATGCTTTGAGAAGAATA+TGG 0.462169 5_4:-14332263 None:intergenic
ATATATACACTTATTAAAAG+TGG 0.471649 5_4:+14331559 Msa0839400:CDS
ACTCCAACAAAAGGAGCAAA+AGG 0.476605 5_4:-14330578 None:intergenic
TCTTGAACTTTAGATATACT+AGG 0.479097 5_4:-14332026 None:intergenic
CAAAGAGTTTCAAGTAGAAG+TGG 0.481571 5_4:+14331258 Msa0839400:CDS
TTTGGGCAGATGCGAGATGT+AGG 0.485083 5_4:+14330486 Msa0839400:CDS
TACTTACATTAATCCCAGCT+TGG 0.488151 5_4:-14330247 None:intergenic
CCTGTAGCGACTCGAAGTAA+AGG 0.488591 5_4:+14331802 Msa0839400:CDS
TAGCATGAAAACACACTGTA+AGG 0.490277 5_4:+14330067 Msa0839400:CDS
AGTTAAAGACATAATGTTCA+AGG 0.493487 5_4:+14331372 Msa0839400:CDS
CTGTAGCGACTCGAAGTAAA+GGG 0.498287 5_4:+14331803 Msa0839400:CDS
ATTGAAAGATAAAATTGAAA+AGG 0.501824 5_4:+14331135 Msa0839400:CDS
TCCCGAAAGCTCGCCATCGA+TGG 0.503126 5_4:+14330771 Msa0839400:CDS
TTTGTTGGAGTAAATCATCA+TGG 0.506547 5_4:+14330590 Msa0839400:CDS
AGGCGGCTTCGACTTGGGAC+AGG 0.507987 5_4:+14330359 Msa0839400:CDS
GTCAATCACATCAAGATCAA+TGG 0.511334 5_4:-14332075 None:intergenic
TTGGGCAGATGCGAGATGTA+GGG 0.512318 5_4:+14330487 Msa0839400:CDS
TGATGATTTACTCCAACAAA+AGG 0.515106 5_4:-14330587 None:intergenic
TGTTTGATGAGCGTCATCGT+TGG 0.518510 5_4:+14330975 Msa0839400:CDS
AAAGTGAGGCGGCTTCGACT+TGG 0.518819 5_4:+14330353 Msa0839400:CDS
TTGGAGGTTAATGACCAAGC+TGG 0.528235 5_4:+14330233 Msa0839400:CDS
ATGATAATGAATGGTTGAAA+GGG 0.531139 5_4:+14330951 Msa0839400:CDS
AAATGATTTATCAAAAGTAA+TGG 0.531224 5_4:+14331675 Msa0839400:CDS
TGGAGGTTAATGACCAAGCT+GGG 0.539674 5_4:+14330234 Msa0839400:CDS
CTCGCCATCGATGGTGCTTG+TGG 0.540244 5_4:+14330780 Msa0839400:CDS
TACTCACATAGCTTCTTGCA+CGG 0.540398 5_4:-14330016 None:intergenic
ATGAACTTCATGATAATGAA+TGG 0.543870 5_4:+14330942 Msa0839400:CDS
AAATGAAGATGATGAACCTA+AGG 0.544931 5_4:+14329857 Msa0839400:CDS
TGGTCAGTAATTATGGCTTG+TGG 0.548697 5_4:-14330713 None:intergenic
AAGTGATTTCATGGAGAAGT+GGG 0.549527 5_4:+14330901 Msa0839400:CDS
GAAGATGATGAACCTAAGGT+TGG 0.554642 5_4:+14329861 Msa0839400:CDS
AAATCCGTAAAAGGCATCGC+AGG 0.562477 5_4:-14331629 None:intergenic
TAAGTGATTTCATGGAGAAG+TGG 0.562788 5_4:+14330900 Msa0839400:CDS
ATGGCGAGCTTTCGGGAAGA+CGG 0.568527 5_4:-14330765 None:intergenic
AATGTATGTGTGTCTTCAGA+TGG 0.571681 5_4:+14330098 Msa0839400:CDS
TGCTTTCTTTGAGAGAATGG+AGG 0.581817 5_4:+14331300 Msa0839400:CDS
GTTATATATTGCCAAGATGG+AGG 0.585753 5_4:+14331521 Msa0839400:CDS
AGGTTGCGCCCTATTGTCGA+AGG 0.587751 5_4:+14330628 Msa0839400:CDS
GCCATAATTACTGACCAAGA+TGG 0.588574 5_4:+14330719 Msa0839400:CDS
TGTTAAGTAAGTGGTGTCGA+AGG 0.588670 5_4:-14330540 None:intergenic
GAAACTACATTGACAAAGTG+AGG 0.593331 5_4:+14330339 Msa0839400:CDS
TATATTGCCAAGATGGAGGA+AGG 0.599926 5_4:+14331525 Msa0839400:CDS
CACTTATCAAAGTCAAGAAG+AGG 0.608354 5_4:+14331921 Msa0839400:CDS
TCTTGGCAATATATAACAAG+AGG 0.613918 5_4:-14331515 None:intergenic
CATGAAATTTAATTCTACAG+AGG 0.616241 5_4:+14329884 Msa0839400:CDS
GTTCTAAATATGAAACATTG+TGG 0.622522 5_4:-14332286 None:intergenic
ACTTGGTTAGAATGTATGCG+TGG 0.623148 5_4:+14330683 Msa0839400:CDS
AGCAATGAAGAATGCAGTTG+AGG 0.631682 5_4:+14330742 Msa0839400:CDS
AATGTGTCTCTCACATACAC+AGG 0.631924 5_4:-14331001 None:intergenic
TCTATGGTTGTTGAAGCAAG+TGG 0.641961 5_4:+14330281 Msa0839400:CDS
CTTGGTTAGAATGTATGCGT+GGG 0.645599 5_4:+14330684 Msa0839400:CDS
CTTGGCAATATATAACAAGA+GGG 0.647143 5_4:-14331514 None:intergenic
TCACATAAATGAAAATTGTG+AGG 0.656952 5_4:+14329821 Msa0839400:CDS
TATGCCACAAGCACCATCGA+TGG 0.658002 5_4:-14330784 None:intergenic
TTGCGCCCTATTGTCGAAGG+AGG 0.658239 5_4:+14330631 Msa0839400:CDS
CTGTGAGCTTAAGAACACAA+AGG 0.694006 5_4:-14331474 None:intergenic
ATATGATAACCTTACATTCG+GGG 0.715139 5_4:+14330304 Msa0839400:CDS
ACTACATTGACAAAGTGAGG+CGG 0.739143 5_4:+14330342 Msa0839400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTATTTTTATGTAG+GGG + chr5_4:14332003-14332022 Msa0839400:intron 10.0%
!! TTTATATGTGTAAATAAAAT+TGG - chr5_4:14332197-14332216 None:intergenic 10.0%
!!! TTAATGTAAGTAGAAATTTT+CGG + chr5_4:14330258-14330277 Msa0839400:CDS 15.0%
!!! TCTAACAAAATAATTTTGAA+TGG - chr5_4:14330396-14330415 None:intergenic 15.0%
!!! TAGTGAATTTTATTATGTAA+TGG + chr5_4:14330430-14330449 Msa0839400:CDS 15.0%
!!! ATATTTATTTGTTAAGTAAG+TGG - chr5_4:14330552-14330571 None:intergenic 15.0%
!! ATTGAAAGATAAAATTGAAA+AGG + chr5_4:14331135-14331154 Msa0839400:CDS 15.0%
!! TAAAATTGAAAAGGAAAATA+TGG + chr5_4:14331144-14331163 Msa0839400:CDS 15.0%
!! ATATATACACTTATTAAAAG+TGG + chr5_4:14331559-14331578 Msa0839400:CDS 15.0%
!! AAATGATTTATCAAAAGTAA+TGG + chr5_4:14331675-14331694 Msa0839400:CDS 15.0%
!!! GAAAAAAGTATTTTACTCTA+GGG + chr5_4:14329988-14330007 Msa0839400:CDS 20.0%
!!! ATTTGGCTTTAAAAGATTTT+TGG - chr5_4:14330045-14330064 None:intergenic 20.0%
!!! ATTTTATTATGTAATGGATG+TGG + chr5_4:14330436-14330455 Msa0839400:CDS 20.0%
!! TATACAAGCAATAATTCTAT+TGG - chr5_4:14331185-14331204 None:intergenic 20.0%
!! ATTTGAATCTCAATTTCAAA+AGG + chr5_4:14331216-14331235 Msa0839400:CDS 20.0%
!!! TTGATAAATCATTTTCAGTA+TGG - chr5_4:14331669-14331688 None:intergenic 20.0%
!!! ATACATTTTATTACGTGTAA+AGG - chr5_4:14331978-14331997 None:intergenic 20.0%
!!! TATTTTTGATTTGGATTGAA+GGG - chr5_4:14332137-14332156 None:intergenic 20.0%
!!! ATATTTTTGATTTGGATTGA+AGG - chr5_4:14332138-14332157 None:intergenic 20.0%
!!! ACATCAAGATATTTTTGATT+TGG - chr5_4:14332146-14332165 None:intergenic 20.0%
!! AAAATATCTTGATGTTAATC+AGG + chr5_4:14332151-14332170 Msa0839400:CDS 20.0%
!! ATAACTCATTCAAAATCTAT+TGG + chr5_4:14332231-14332250 Msa0839400:intron 20.0%
!!! ATTCAAAATCTATTGGTTTT+AGG + chr5_4:14332238-14332257 Msa0839400:intron 20.0%
!!! TTCATATTTAGAACTTTTAC+AGG + chr5_4:14332295-14332314 Msa0839400:CDS 20.0%
!! ATGATGAATGATCATTTATA+TGG - chr5_4:14332412-14332431 None:intergenic 20.0%
! TCACATAAATGAAAATTGTG+AGG + chr5_4:14329821-14329840 Msa0839400:CDS 25.0%
! CATGAAATTTAATTCTACAG+AGG + chr5_4:14329884-14329903 Msa0839400:CDS 25.0%
! AAGAATTATGCAAGATGTTT+AGG + chr5_4:14329924-14329943 Msa0839400:CDS 25.0%
!!! GGAAAAAAGTATTTTACTCT+AGG + chr5_4:14329987-14330006 Msa0839400:CDS 25.0%
!!! TGGAACAATTACTATTTCTA+AGG + chr5_4:14330118-14330137 Msa0839400:CDS 25.0%
!!! CTAAAATATCTTGATTTGCT+TGG - chr5_4:14330176-14330195 None:intergenic 25.0%
! ATATAGACCCTAGTATAAAA+AGG + chr5_4:14330207-14330226 Msa0839400:CDS 25.0%
!!! TCGTTTACGAAATGTTTTTT+GGG + chr5_4:14330469-14330488 Msa0839400:CDS 25.0%
!!! AGGATACTAAAACTTTTTCT+TGG + chr5_4:14330651-14330670 Msa0839400:CDS 25.0%
!! ATGAACTTCATGATAATGAA+TGG + chr5_4:14330942-14330961 Msa0839400:CDS 25.0%
!! ATGATAATGAATGGTTGAAA+GGG + chr5_4:14330951-14330970 Msa0839400:CDS 25.0%
!! ATTGCTTGTATAAGTCATTT+TGG + chr5_4:14331193-14331212 Msa0839400:CDS 25.0%
!! AAACTCTTTGAATTTTGCAT+TGG - chr5_4:14331248-14331267 None:intergenic 25.0%
! TTGTGTTACAGAAAGTAAAA+AGG + chr5_4:14331339-14331358 Msa0839400:CDS 25.0%
! AGTTAAAGACATAATGTTCA+AGG + chr5_4:14331372-14331391 Msa0839400:CDS 25.0%
!! ATGCTGTTTATTTGAGTTTA+AGG + chr5_4:14331432-14331451 Msa0839400:CDS 25.0%
!! TGCTGTTTATTTGAGTTTAA+GGG + chr5_4:14331433-14331452 Msa0839400:CDS 25.0%
!! ACACTTATTAAAAGTGGTTT+TGG + chr5_4:14331565-14331584 Msa0839400:CDS 25.0%
! TAGAATAGAGAACTTAAAAC+TGG + chr5_4:14331699-14331718 Msa0839400:CDS 25.0%
! TCTTGAACTTTAGATATACT+AGG - chr5_4:14332029-14332048 None:intergenic 25.0%
!! GTTCTAAATATGAAACATTG+TGG - chr5_4:14332289-14332308 None:intergenic 25.0%
AAATGAAGATGATGAACCTA+AGG + chr5_4:14329857-14329876 Msa0839400:CDS 30.0%
TTAAATTTCATGCCAACCTT+AGG - chr5_4:14329876-14329895 None:intergenic 30.0%
!! TATGCAAGATGTTTAGGTTT+TGG + chr5_4:14329930-14329949 Msa0839400:CDS 30.0%
TAGCACAATCAGTACAAAAA+AGG + chr5_4:14329956-14329975 Msa0839400:CDS 30.0%
AGCACAATCAGTACAAAAAA+GGG + chr5_4:14329957-14329976 Msa0839400:CDS 30.0%
! CCAATCTCCTTTTTATACTA+GGG - chr5_4:14330217-14330236 None:intergenic 30.0%
! TCCAATCTCCTTTTTATACT+AGG - chr5_4:14330218-14330237 None:intergenic 30.0%
TAGTATAAAAAGGAGATTGG+AGG + chr5_4:14330217-14330236 Msa0839400:CDS 30.0%
! AAGTAGAAATTTTCGGTCTA+TGG + chr5_4:14330265-14330284 Msa0839400:CDS 30.0%
GGATATGATAACCTTACATT+CGG + chr5_4:14330302-14330321 Msa0839400:CDS 30.0%
GATATGATAACCTTACATTC+GGG + chr5_4:14330303-14330322 Msa0839400:CDS 30.0%
ATATGATAACCTTACATTCG+GGG + chr5_4:14330304-14330323 Msa0839400:CDS 30.0%
!!! GTCGTTTACGAAATGTTTTT+TGG + chr5_4:14330468-14330487 Msa0839400:CDS 30.0%
TGATGATTTACTCCAACAAA+AGG - chr5_4:14330590-14330609 None:intergenic 30.0%
TTTGTTGGAGTAAATCATCA+TGG + chr5_4:14330590-14330609 Msa0839400:CDS 30.0%
CTTGTGGCATATAATGAAAA+AGG + chr5_4:14330796-14330815 Msa0839400:CDS 30.0%
! CAAAATACTTTTGCATGATG+CGG + chr5_4:14330859-14330878 Msa0839400:CDS 30.0%
TTTGAGCATAAGTGATTTCA+TGG + chr5_4:14330892-14330911 Msa0839400:CDS 30.0%
! CATGATAATGAATGGTTGAA+AGG + chr5_4:14330950-14330969 Msa0839400:CDS 30.0%
!!! AGTATGAACGCATTTTTTGA+TGG + chr5_4:14331058-14331077 Msa0839400:CDS 30.0%
CTTGGCAATATATAACAAGA+GGG - chr5_4:14331517-14331536 None:intergenic 30.0%
TCTTGGCAATATATAACAAG+AGG - chr5_4:14331518-14331537 None:intergenic 30.0%
! CTTGTTATATATTGCCAAGA+TGG + chr5_4:14331518-14331537 Msa0839400:CDS 30.0%
! GACATGTCAAGAAAGTTTTT+CGG + chr5_4:14331726-14331745 Msa0839400:CDS 30.0%
!! TCTATGCTTTGAGAAGAATA+TGG - chr5_4:14332266-14332285 None:intergenic 30.0%
AGTACAAAAAAGGGAGATGA+TGG + chr5_4:14329966-14329985 Msa0839400:CDS 35.0%
! TGTGTTTTCATGCTAGGATT+TGG - chr5_4:14330062-14330081 None:intergenic 35.0%
! TTACAGTGTGTTTTCATGCT+AGG - chr5_4:14330068-14330087 None:intergenic 35.0%
TAGCATGAAAACACACTGTA+AGG + chr5_4:14330067-14330086 Msa0839400:CDS 35.0%
AATGTATGTGTGTCTTCAGA+TGG + chr5_4:14330098-14330117 Msa0839400:CDS 35.0%
CCCTAGTATAAAAAGGAGAT+TGG + chr5_4:14330214-14330233 Msa0839400:CDS 35.0%
TACTTACATTAATCCCAGCT+TGG - chr5_4:14330250-14330269 None:intergenic 35.0%
GAAACTACATTGACAAAGTG+AGG + chr5_4:14330339-14330358 Msa0839400:CDS 35.0%
TTTCTTGGTTGTTCACTACT+TGG + chr5_4:14330666-14330685 Msa0839400:CDS 35.0%
TCCATCTTGGTCAGTAATTA+TGG - chr5_4:14330723-14330742 None:intergenic 35.0%
! GAGAGTCTACTCAATTTTTC+TGG - chr5_4:14330824-14330843 None:intergenic 35.0%
TAAGTGATTTCATGGAGAAG+TGG + chr5_4:14330900-14330919 Msa0839400:CDS 35.0%
AAGTGATTTCATGGAGAAGT+GGG + chr5_4:14330901-14330920 Msa0839400:CDS 35.0%
! TGTATGTGAGAGACACATTT+TGG + chr5_4:14331005-14331024 Msa0839400:CDS 35.0%
! GTATGTGAGAGACACATTTT+GGG + chr5_4:14331006-14331025 Msa0839400:CDS 35.0%
! CAAAGAGTTTCAAGTAGAAG+TGG + chr5_4:14331258-14331277 Msa0839400:CDS 35.0%
! TCATGCTTTCTTTGAGAGAA+TGG + chr5_4:14331297-14331316 Msa0839400:CDS 35.0%
GTTATATATTGCCAAGATGG+AGG + chr5_4:14331521-14331540 Msa0839400:CDS 35.0%
TTAATATCCTTCCTCCATCT+TGG - chr5_4:14331535-14331554 None:intergenic 35.0%
TGAAGAAGCAAATCCGTAAA+AGG - chr5_4:14331641-14331660 None:intergenic 35.0%
! TTAGAAGCCTTCCTTTTTGA+AGG - chr5_4:14331835-14331854 None:intergenic 35.0%
ATTGTGAAAAAGAAGCTTGC+TGG + chr5_4:14331865-14331884 Msa0839400:CDS 35.0%
CACTTATCAAAGTCAAGAAG+AGG + chr5_4:14331921-14331940 Msa0839400:CDS 35.0%
GTCAATCACATCAAGATCAA+TGG - chr5_4:14332078-14332097 None:intergenic 35.0%
GAAGATGATGAACCTAAGGT+TGG + chr5_4:14329861-14329880 Msa0839400:CDS 40.0%
TACTCACATAGCTTCTTGCA+CGG - chr5_4:14330019-14330038 None:intergenic 40.0%
TCTATGGTTGTTGAAGCAAG+TGG + chr5_4:14330281-14330300 Msa0839400:CDS 40.0%
! CATCTTTTTCCCCGAATGTA+AGG - chr5_4:14330316-14330335 None:intergenic 40.0%
ACTACATTGACAAAGTGAGG+CGG + chr5_4:14330342-14330361 Msa0839400:CDS 40.0%
! AGGGCTGCTTATGAGTATTT+TGG + chr5_4:14330506-14330525 Msa0839400:CDS 40.0%
! TGTTAAGTAAGTGGTGTCGA+AGG - chr5_4:14330543-14330562 None:intergenic 40.0%
! ATGCCTTTTGCTCCTTTTGT+TGG + chr5_4:14330575-14330594 Msa0839400:CDS 40.0%
ACTCCAACAAAAGGAGCAAA+AGG - chr5_4:14330581-14330600 None:intergenic 40.0%
AGTATCCTCCTTCGACAATA+GGG - chr5_4:14330639-14330658 None:intergenic 40.0%
TAGTATCCTCCTTCGACAAT+AGG - chr5_4:14330640-14330659 None:intergenic 40.0%
ACTTGGTTAGAATGTATGCG+TGG + chr5_4:14330683-14330702 Msa0839400:CDS 40.0%
CTTGGTTAGAATGTATGCGT+GGG + chr5_4:14330684-14330703 Msa0839400:CDS 40.0%
TGGTCAGTAATTATGGCTTG+TGG - chr5_4:14330716-14330735 None:intergenic 40.0%
GCCATAATTACTGACCAAGA+TGG + chr5_4:14330719-14330738 Msa0839400:CDS 40.0%
CATTCTTCATTGCTCCATCT+TGG - chr5_4:14330736-14330755 None:intergenic 40.0%
AGCAATGAAGAATGCAGTTG+AGG + chr5_4:14330742-14330761 Msa0839400:CDS 40.0%
AATGTGTCTCTCACATACAC+AGG - chr5_4:14331004-14331023 None:intergenic 40.0%
! TGCTTTCTTTGAGAGAATGG+AGG + chr5_4:14331300-14331319 Msa0839400:CDS 40.0%
CTGTGAGCTTAAGAACACAA+AGG - chr5_4:14331477-14331496 None:intergenic 40.0%
TATATTGCCAAGATGGAGGA+AGG + chr5_4:14331525-14331544 Msa0839400:CDS 40.0%
!!! AGTGGTTTTGGTCAATTAGC+CGG + chr5_4:14331577-14331596 Msa0839400:CDS 40.0%
!! AGTTTTTCGGAGATCACTGA+AGG + chr5_4:14331739-14331758 Msa0839400:CDS 40.0%
!! AAGAAGCTTGCTGGAAAGAA+AGG + chr5_4:14331874-14331893 Msa0839400:CDS 40.0%
!! CATTCGTGTGTACTTTTTGC+AGG + chr5_4:14332379-14332398 Msa0839400:intron 40.0%
TTGGAGGTTAATGACCAAGC+TGG + chr5_4:14330233-14330252 Msa0839400:CDS 45.0%
TGGAGGTTAATGACCAAGCT+GGG + chr5_4:14330234-14330253 Msa0839400:CDS 45.0%
CATGGTCAGTCAGTTCTGTT+AGG + chr5_4:14330608-14330627 Msa0839400:CDS 45.0%
TGTTTGATGAGCGTCATCGT+TGG + chr5_4:14330975-14330994 Msa0839400:CDS 45.0%
GTTTGATGAGCGTCATCGTT+GGG + chr5_4:14330976-14330995 Msa0839400:CDS 45.0%
ACGCTGCAACTCTTCATTTC+CGG - chr5_4:14331599-14331618 None:intergenic 45.0%
! AAAGCCTGCGATGCCTTTTA+CGG + chr5_4:14331625-14331644 Msa0839400:CDS 45.0%
!! AAATCCGTAAAAGGCATCGC+AGG - chr5_4:14331632-14331651 None:intergenic 45.0%
CTGTAGCGACTCGAAGTAAA+GGG + chr5_4:14331803-14331822 Msa0839400:CDS 45.0%
!! GAAGCCTTCCTTTTTGAAGG+AGG - chr5_4:14331832-14331851 None:intergenic 45.0%
!!! TAATTTATTTATTTTTATGT+AGG + chr5_4:14332001-14332020 Msa0839400:intron 5.0%
!!! AATTTATTTATTTTTATGTA+GGG + chr5_4:14332002-14332021 Msa0839400:intron 5.0%
TTTGGGCAGATGCGAGATGT+AGG + chr5_4:14330486-14330505 Msa0839400:CDS 50.0%
TTGGGCAGATGCGAGATGTA+GGG + chr5_4:14330487-14330506 Msa0839400:CDS 50.0%
TATGCCACAAGCACCATCGA+TGG - chr5_4:14330787-14330806 None:intergenic 50.0%
! GTGAGAGACACATTTTGGGC+TGG + chr5_4:14331010-14331029 Msa0839400:CDS 50.0%
CCTTTACTTCGAGTCGCTAC+AGG - chr5_4:14331805-14331824 None:intergenic 50.0%
CCTGTAGCGACTCGAAGTAA+AGG + chr5_4:14331802-14331821 Msa0839400:CDS 50.0%
!! AAGGGCGTCCTCCTTCAAAA+AGG + chr5_4:14331821-14331840 Msa0839400:CDS 50.0%
GCGTCCTCCTTCAAAAAGGA+AGG + chr5_4:14331825-14331844 Msa0839400:CDS 50.0%
AAAGTGAGGCGGCTTCGACT+TGG + chr5_4:14330353-14330372 Msa0839400:CDS 55.0%
AAGTGAGGCGGCTTCGACTT+GGG + chr5_4:14330354-14330373 Msa0839400:CDS 55.0%
AGGTTGCGCCCTATTGTCGA+AGG + chr5_4:14330628-14330647 Msa0839400:CDS 55.0%
TTGCGCCCTATTGTCGAAGG+AGG + chr5_4:14330631-14330650 Msa0839400:CDS 55.0%
ATGGCGAGCTTTCGGGAAGA+CGG - chr5_4:14330768-14330787 None:intergenic 55.0%
ACCATCGATGGCGAGCTTTC+GGG - chr5_4:14330775-14330794 None:intergenic 55.0%
CACCATCGATGGCGAGCTTT+CGG - chr5_4:14330776-14330795 None:intergenic 55.0%
TCCCGAAAGCTCGCCATCGA+TGG + chr5_4:14330771-14330790 Msa0839400:CDS 60.0%
CTCGCCATCGATGGTGCTTG+TGG + chr5_4:14330780-14330799 Msa0839400:CDS 60.0%
!! AGGCGGCTTCGACTTGGGAC+AGG + chr5_4:14330359-14330378 Msa0839400:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 14329777 14332436 14329777 ID=Msa0839400;Name=Msa0839400
chr5_4 mRNA 14329777 14332436 14329777 ID=Msa0839400-mRNA-1;Parent=Msa0839400;Name=Msa0839400-mRNA-1;_AED=0.25;_eAED=0.37;_QI=0|0|0|0.25|1|1|4|0|802
chr5_4 exon 14329777 14331942 14329777 ID=Msa0839400-mRNA-1:exon:6272;Parent=Msa0839400-mRNA-1
chr5_4 exon 14332023 14332172 14332023 ID=Msa0839400-mRNA-1:exon:6273;Parent=Msa0839400-mRNA-1
chr5_4 exon 14332260 14332316 14332260 ID=Msa0839400-mRNA-1:exon:6274;Parent=Msa0839400-mRNA-1
chr5_4 exon 14332401 14332436 14332401 ID=Msa0839400-mRNA-1:exon:6275;Parent=Msa0839400-mRNA-1
chr5_4 CDS 14329777 14331942 14329777 ID=Msa0839400-mRNA-1:cds;Parent=Msa0839400-mRNA-1
chr5_4 CDS 14332023 14332172 14332023 ID=Msa0839400-mRNA-1:cds;Parent=Msa0839400-mRNA-1
chr5_4 CDS 14332260 14332316 14332260 ID=Msa0839400-mRNA-1:cds;Parent=Msa0839400-mRNA-1
chr5_4 CDS 14332401 14332436 14332401 ID=Msa0839400-mRNA-1:cds;Parent=Msa0839400-mRNA-1
Gene Sequence

>Msa0839400

GAGAAAACTTTTAGATCTTTTGAAGAAGATGAAGAAGAGAGTGATCACATAAATGAAAATTGTGAGGATAATTTAACAGAAAATGAAGATGATGAACCTAAGGTTGGCATGAAATTTAATTCTACAGAGGAAGTTGACAAATATTACAAGAATTATGCAAGATGTTTAGGTTTTGGAGTTAGCACAATCAGTACAAAAAAGGGAGATGATGGAAAAAAGTATTTTACTCTAGGGTGTAACCGTGCAAGAAGCTATGTGAGTAAGTCCAAAAATCTTTTAAAGCCAAATCCTAGCATGAAAACACACTGTAAGGCTAGAATAAATGTATGTGTGTCTTCAGATGGAACAATTACTATTTCTAAGGTTGCTCTTGAACATAATCATGACTTAATAAGTCCAAGCAAATCAAGATATTTTAGATGCAACAAGAATATAGACCCTAGTATAAAAAGGAGATTGGAGGTTAATGACCAAGCTGGGATTAATGTAAGTAGAAATTTTCGGTCTATGGTTGTTGAAGCAAGTGGATATGATAACCTTACATTCGGGGAAAAAGATGTTAGAAACTACATTGACAAAGTGAGGCGGCTTCGACTTGGGACAGGAGACGCTGAAGCCATTCAAAATTATTTTGTTAGAATGCAAAGACAAAATAGTGAATTTTATTATGTAATGGATGTGGATGACAAAAGTCGTTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGGCTGCTTATGAGTATTTTGGTGAAGTAATAACCTTCGACACCACTTACTTAACAAATAAATATGACATGCCTTTTGCTCCTTTTGTTGGAGTAAATCATCATGGTCAGTCAGTTCTGTTAGGTTGCGCCCTATTGTCGAAGGAGGATACTAAAACTTTTTCTTGGTTGTTCACTACTTGGTTAGAATGTATGCGTGGGCGTGCTCCACAAGCCATAATTACTGACCAAGATGGAGCAATGAAGAATGCAGTTGAGGCCGTCTTCCCGAAAGCTCGCCATCGATGGTGCTTGTGGCATATAATGAAAAAGGTTCCAGAAAAATTGAGTAGACTCTCTAACTATGAGTCTATCAAAATACTTTTGCATGATGCGGTGTATGATTCTTTGAGCATAAGTGATTTCATGGAGAAGTGGGAAAAAATGATTGAATGTTATGAACTTCATGATAATGAATGGTTGAAAGGGTTGTTTGATGAGCGTCATCGTTGGGTTCCTGTGTATGTGAGAGACACATTTTGGGCTGGAATGTCAACAACACAACGAAGTGAAAGTATGAACGCATTTTTTGATGGATATGTGCACTCAAAGACATCATTGAAGCAATTTGTTGAACAATATGATAATGCATTGAAAGATAAAATTGAAAAGGAAAATATGGCTGATTTTAGTTCATCCAATAGAATTATTGCTTGTATAAGTCATTTTGGATTTGAATCTCAATTTCAAAAGGCATATACCAATGCAAAATTCAAAGAGTTTCAAGTAGAAGTGGCTTCTATGATGTACTGTCATGCTTTCTTTGAGAGAATGGAGGCTTTGAACTTAACATATTGTGTTACAGAAAGTAAAAAGGTATTTGATACAGTTAAAGACATAATGTTCAAGGTATTCTTCAATGAGAAAGACTTTGAAATACGATGTACATGCTGTTTATTTGAGTTTAAGGGTATTTTATGTAGACATATCCTTTGTGTTCTTAAGCTCACAGCTAAAACAGAGTCAGTGCCCTCTTGTTATATATTGCCAAGATGGAGGAAGGATATTAAGCGAATATATACACTTATTAAAAGTGGTTTTGGTCAATTAGCCGGAAATGAAGAGTTGCAGCGTGTAGCTAAAGCCTGCGATGCCTTTTACGGATTTGCTTCTTCATGTATCCATACTGAAAATGATTTATCAAAAGTAATGGATAGAATAGAGAACTTAAAACTGGAGATGACATGTCAAGAAAGTTTTTCGGAGATCACTGAAGGAGATAACTTAGTTCAAAATCAGATGACTAAAATTCTTGATCCTGTAGCGACTCGAAGTAAAGGGCGTCCTCCTTCAAAAAGGAAGGCTTCTAAAGTTGATCAAATTGTGAAAAAGAAGCTTGCTGGAAAGAAAGGACAAAAAAGCAACAAAAAAAGTAACACTTATCAAAGTCAAGAAGAGGGGCCTAGTATATCTAAAGTTCAAGAAAATCAAGAGTTCTCTATATCTCAGACCATTGATCTTGATGTGATTGACACACAAGAGAGCATTCAAGTAAAACAAATGTTAGCTCCCTTCAATCCAAATCAAAAATATCTTGATGTTAATCAGGTTCCATATTCTTCTCAAAGCATAGACCACAATGTTTCATATTTAGAACTTTTACAGGCACAACACCATATAAATGATCATTCATCATCATGA
Protein sequence

>Msa0839400

EKTFRSFEEDEEESDHINENCEDNLTENEDDEPKVGMKFNSTEEVDKYYKNYARCLGFGVSTISTKKGDDGKKYFTLGCNRARSYVSKSKNLLKPNPSMKTHCKARINVCVSSDGTITISKVALEHNHDLISPSKSRYFRCNKNIDPSIKRRLEVNDQAGINVSRNFRSMVVEASGYDNLTFGEKDVRNYIDKVRRLRLGTGDAEAIQNYFVRMQRQNSEFYYVMDVDDKSRLRNVFWADARCRAAYEYFGEVITFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSKEDTKTFSWLFTTWLECMRGRAPQAIITDQDGAMKNAVEAVFPKARHRWCLWHIMKKVPEKLSRLSNYESIKILLHDAVYDSLSISDFMEKWEKMIECYELHDNEWLKGLFDERHRWVPVYVRDTFWAGMSTTQRSESMNAFFDGYVHSKTSLKQFVEQYDNALKDKIEKENMADFSSSNRIIACISHFGFESQFQKAYTNAKFKEFQVEVASMMYCHAFFERMEALNLTYCVTESKKVFDTVKDIMFKVFFNEKDFEIRCTCCLFEFKGILCRHILCVLKLTAKTESVPSCYILPRWRKDIKRIYTLIKSGFGQLAGNEELQRVAKACDAFYGFASSCIHTENDLSKVMDRIENLKLEMTCQESFSEITEGDNLVQNQMTKILDPVATRSKGRPPSKRKASKVDQIVKKKLAGKKGQKSNKKSNTYQSQEEGPSISKVQENQEFSISQTIDLDVIDTQESIQVKQMLAPFNPNQKYLDVNQVPYSSQSIDHNVSYLELLQAQHHINDHSSS*