AlfalfaGEDB | Msa0840660

M. sativa cultivar zhongmu-4 / Msa0840660

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840660	XP_003612667.1	89.787	235	21	2	3	237	2	233	1.70e-143	410

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840660	sp\|Q8S9H7\|DIV_ANTMA	39.227	181	91	5	30	204	65	232	2.00e-26	107

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840660	G7K885	89.787	235	21	2	3	237	2	233	8.10e-144	410

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TFs/TRs:

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Gene ID	Type	Classification
Msa0840660	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840660	MtrunA17_Chr5g0409841	89.787	235	21	2	3	237	2	233	1.56e-147	410
Msa0840660	MtrunA17_Chr5g0440731	57.597	283	64	5	3	235	18	294	1.55e-92	273
Msa0840660	MtrunA17_Chr5g0440741	57.597	283	59	7	3	235	18	289	5.36e-90	267
Msa0840660	MtrunA17_Chr5g0409831	58.367	245	73	5	3	235	2	229	1.77e-83	248
Msa0840660	MtrunA17_Chr1g0210471	42.000	150	69	4	59	204	85	220	3.54e-26	102
Msa0840660	MtrunA17_Chr1g0162081	54.444	90	38	2	82	171	110	196	1.86e-25	101
Msa0840660	MtrunA17_Chr5g0436501	38.286	175	90	4	30	193	66	233	1.42e-24	98.2
Msa0840660	MtrunA17_Chr4g0062741	32.683	205	105	6	29	206	45	243	2.84e-24	98.2
Msa0840660	MtrunA17_Chr7g0241761	44.882	127	57	4	84	208	129	244	1.75e-23	96.3
Msa0840660	MtrunA17_Chr3g0104071	44.715	123	60	2	85	207	137	251	6.19e-23	94.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0840660	AT5G08520.1	45.161	124	54	2	60	171	71	192	2.31e-26	103
Msa0840660	AT3G10580.2	33.690	187	99	6	22	206	39	202	3.06e-24	97.4
Msa0840660	AT5G23650.1	39.264	163	86	4	55	217	83	232	7.01e-24	98.2
Msa0840660	AT5G04760.1	54.217	83	36	1	80	162	84	164	7.07e-24	95.5
Msa0840660	AT3G10580.1	33.690	187	99	6	22	206	39	202	9.01e-24	97.1
Msa0840660	AT5G58900.1	61.333	75	26	2	91	164	137	209	3.33e-23	95.5
Msa0840660	AT1G49010.1	51.163	86	39	2	87	171	126	209	4.35e-23	95.5
Msa0840660	AT2G38090.1	54.878	82	34	2	84	164	130	209	1.33e-21	91.3
Msa0840660	AT3G11280.2	41.538	130	68	3	83	206	115	242	1.50e-21	90.5
Msa0840660	AT3G11280.1	41.538	130	68	3	83	206	115	242	1.50e-21	90.5

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Find 42 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGAGGGCGTAGGGAAA+GGG	0.499642	5_4:+16507954	Msa0840660:CDS
AATGAGCGGACTTCAATTCA+AGG	0.465890	5_4:+16507742	Msa0840660:CDS
AGATATTTCAAAAGAATTTG+TGG	0.448838	5_4:+16508776	Msa0840660:CDS
AGGTCCCTCTGAGAATAGCA+TGG	0.632380	5_4:+16507762	Msa0840660:CDS
ATCATATCGATTTAAATGAG+CGG	0.624082	5_4:+16507728	Msa0840660:CDS
ATGTATAGGTTGTTTCTGGA+GGG	0.542704	5_4:+16508723	Msa0840660:intron
ATGTGAAAGATGTAGAGAAG+AGG	0.526828	5_4:+16508856	Msa0840660:CDS
ATTAGAGTTTAGCATTGCGG+CGG	0.651248	5_4:-16507836	None:intergenic
CAAAATGAAACTCCTCCTCA+AGG	0.584892	5_4:+16508978	Msa0840660:CDS
CAAAGAGAGGGCGTAGGGAA+AGG	0.497543	5_4:+16507953	Msa0840660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCATTAGAGTTTAGCATTG+CGG	-	chr5_4:16507842-16507861	None:intergenic	30.0%
GTAAGTATTCAATCTATCCT+TGG	+	chr5_4:16508002-16508021	Msa0840660:intron	30.0%
TTCTCAAGTTTCTCTATATC+CGG	-	chr5_4:16509080-16509099	None:intergenic	30.0%
TTCATTAGAGTTTAGCATTG+CGG	-	chr5_4:16507842-16507861	None:intergenic	30.0%
GTAAGTATTCAATCTATCCT+TGG	+	chr5_4:16508002-16508021	Msa0840660:intron	30.0%
TTCTCAAGTTTCTCTATATC+CGG	-	chr5_4:16509080-16509099	None:intergenic	30.0%
TTAGCTTCATTACTTCCTTC+AGG	-	chr5_4:16507892-16507911	None:intergenic	35.0%
GGAAGTAATGAAGCTAACTT+AGG	+	chr5_4:16507895-16507914	Msa0840660:CDS	35.0%
AATGTTAACTAGTGTCCCAA+GGG	-	chr5_4:16508195-16508214	None:intergenic	35.0%
TAATGTTAACTAGTGTCCCA+AGG	-	chr5_4:16508196-16508215	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	16507694	16509136	16507694	ID=Msa0840660;Name=Msa0840660
chr5_4	mRNA	16507694	16509136	16507694	ID=Msa0840660-mRNA-1;Parent=Msa0840660;Name=Msa0840660-mRNA-1;_AED=0.01;_eAED=0.03;_QI=0\|0\|0\|1\|1\|1\|2\|0\|237
chr5_4	exon	16507694	16508001	16507694	ID=Msa0840660-mRNA-1:exon:7084;Parent=Msa0840660-mRNA-1
chr5_4	exon	16508731	16509136	16508731	ID=Msa0840660-mRNA-1:exon:7085;Parent=Msa0840660-mRNA-1
chr5_4	CDS	16507694	16508001	16507694	ID=Msa0840660-mRNA-1:cds;Parent=Msa0840660-mRNA-1
chr5_4	CDS	16508731	16509136	16508731	ID=Msa0840660-mRNA-1:cds;Parent=Msa0840660-mRNA-1

Gene Sequence

>Msa0840660

ATGGAAGAAGGAAGAAAAGTGCAACGATTACCTCATCATATCGATTTAAATGAGCGGACTTCAATTCAAGGTCCCTCTGAGAATAGCATGGAACAACAACAAGAGAAACTTGACGACACTGATGTCATTGAGTCGGCTCATGCCGCCGCAATGCTAAACTCTAATGAAAATAACTCAGTTCAAGCTGAGTCAGTACCTGAAGGAAGTAATGAAGCTAACTTAGGTCAAGCTGAGTCAGTACAAGAAGGAAGTCGTGAAGCAAAGAGAGGGCGTAGGGAAAGGGTACATTGGACGGAAGGTGAACATAAGTTGTTTCTGGAGGGGCTAGAAAAATATGGTAAGGGACGTTGGAAAGATATTTCAAAAGAATTTGTGGTGACAAAGACTCCAATTCAAATAGCTAGCCATGCCCAAAAATACTTTATCCACCAAAATGTGAAAGATGTAGAGAAGAGGAAGAAGAGAAGAAGTATTCATGACACAAGCTTAAACAAGAATGGCACACTAGTCACTCTTGCTGTTGACCAACAAGATGAGATTCCTTCCATTGAGCAGCAAAATGAAACTCCTCCTCAAGGAATGCAACAGACACAAACACAACAAAATGAGATTTCTCCAATGTTGTGCTTGCTTTGTCCAATCGGTTCTACAATTCCGGATATAGAGAAACTTGAGAAGATGCGCGACTTATTGGCAAAAGAACTTCGACGCTAG

Protein sequence

>Msa0840660

MEEGRKVQRLPHHIDLNERTSIQGPSENSMEQQQEKLDDTDVIESAHAAAMLNSNENNSVQAESVPEGSNEANLGQAESVQEGSREAKRGRRERVHWTEGEHKLFLEGLEKYGKGRWKDISKEFVVTKTPIQIASHAQKYFIHQNVKDVEKRKKRRSIHDTSLNKNGTLVTLAVDQQDEIPSIEQQNETPPQGMQQTQTQQNEISPMLCLLCPIGSTIPDIEKLEKMRDLLAKELRR*