Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0845170 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0845170 | MtrunA17_Chr6g0460941 | 82.143 | 28 | 5 | 0 | 12 | 39 | 10 | 37 | 9.00e-11 | 52.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGCCGTGCACAAGGTCTT+TGG | 0.247339 | 5_4:-24106032 | Msa0845170:CDS |
| GGCAAAAGCTAGCTGCATTT+TGG | 0.257694 | 5_4:+24106052 | None:intergenic |
| TTGAAGAGGAAGTGTATTAA+TGG | 0.327862 | 5_4:-24106105 | Msa0845170:CDS |
| TGCATCTTTGCACCCTACTT+TGG | 0.399115 | 5_4:-24106081 | Msa0845170:CDS |
| CTCTTCAAGTGCTTGCAAGC+AGG | 0.450520 | 5_4:+24106120 | None:intergenic |
| GTGCACAAGGTCTTTGGAGC+TGG | 0.472916 | 5_4:-24106026 | Msa0845170:CDS |
| GCTTCTTTGCGGTGGTTAGT+TGG | 0.473263 | 5_4:+24105975 | None:intergenic |
| CAGATATATCGGTGCTGTGT+GGG | 0.478200 | 5_4:-24106144 | Msa0845170:CDS |
| ATTCTCTGTGGCAGATATAT+CGG | 0.488685 | 5_4:-24106155 | Msa0845170:intron |
| GCGGTGGTTAGTTGGAATAG+TGG | 0.521356 | 5_4:+24105983 | None:intergenic |
| AGCTCCAAAGACCTTGTGCA+CGG | 0.586947 | 5_4:+24106028 | None:intergenic |
| CTGCTTGCAAGCACTTGAAG+AGG | 0.588240 | 5_4:-24106119 | Msa0845170:CDS |
| GCAGATATATCGGTGCTGTG+TGG | 0.624107 | 5_4:-24106145 | Msa0845170:intron |
| AGATATATCGGTGCTGTGTG+GGG | 0.631275 | 5_4:-24106143 | Msa0845170:CDS |
| TCCAAAGACCTTGTGCACGG+CGG | 0.712208 | 5_4:+24106031 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATATGTGATGATTTATGA+GGG | + | chr5_4:24106010-24106029 | None:intergenic | 20.0% |
| !! | TTTATATGTGATGATTTATG+AGG | + | chr5_4:24106011-24106030 | None:intergenic | 20.0% |
| !!! | CATTTATTAATGTTTTAGTG+TGG | - | chr5_4:24106089-24106108 | Msa0845170:CDS | 20.0% |
| ! | TATATGTGATGATTTATGAG+GGG | + | chr5_4:24106009-24106028 | None:intergenic | 25.0% |
| ! | TCGCAATTTATAACATTGTT+GGG | + | chr5_4:24106040-24106059 | None:intergenic | 25.0% |
| ! | ATCGCAATTTATAACATTGT+TGG | + | chr5_4:24106041-24106060 | None:intergenic | 25.0% |
| ! | AATTCAACCGATATAACTAT+AGG | + | chr5_4:24106119-24106138 | None:intergenic | 25.0% |
| CAATTTATAACATTGTTGGG+TGG | + | chr5_4:24106037-24106056 | None:intergenic | 30.0% | |
| ! | TTGAAGAGGAAGTGTATTAA+TGG | - | chr5_4:24106197-24106216 | Msa0845170:intron | 30.0% |
| GTTGTGACTACATATAGTTG+TGG | + | chr5_4:24106070-24106089 | None:intergenic | 35.0% | |
| AATTTGTCTGTGATTCTCTG+TGG | - | chr5_4:24106135-24106154 | Msa0845170:CDS | 35.0% | |
| ATTCTCTGTGGCAGATATAT+CGG | - | chr5_4:24106147-24106166 | Msa0845170:intron | 35.0% | |
| ! | TTTTGGTCTGTACCAAAGTA+GGG | + | chr5_4:24106236-24106255 | None:intergenic | 35.0% |
| !! | ATTTTGGTCTGTACCAAAGT+AGG | + | chr5_4:24106237-24106256 | None:intergenic | 35.0% |
| TGGCACGCCTATAGTTATAT+CGG | - | chr5_4:24106109-24106128 | Msa0845170:CDS | 40.0% | |
| TTTATGAGGGGAGTGAACGT+TGG | + | chr5_4:24105997-24106016 | None:intergenic | 45.0% | |
| !! | CAGATATATCGGTGCTGTGT+GGG | - | chr5_4:24106158-24106177 | Msa0845170:intron | 45.0% |
| !! | AGATATATCGGTGCTGTGTG+GGG | - | chr5_4:24106159-24106178 | Msa0845170:intron | 45.0% |
| TGCATCTTTGCACCCTACTT+TGG | - | chr5_4:24106221-24106240 | Msa0845170:intron | 45.0% | |
| ! | GGCAAAAGCTAGCTGCATTT+TGG | + | chr5_4:24106253-24106272 | None:intergenic | 45.0% |
| !! | GCAGATATATCGGTGCTGTG+TGG | - | chr5_4:24106157-24106176 | Msa0845170:intron | 50.0% |
| CTCTTCAAGTGCTTGCAAGC+AGG | + | chr5_4:24106185-24106204 | None:intergenic | 50.0% | |
| CTGCTTGCAAGCACTTGAAG+AGG | - | chr5_4:24106183-24106202 | Msa0845170:intron | 50.0% | |
| AGCTCCAAAGACCTTGTGCA+CGG | + | chr5_4:24106277-24106296 | None:intergenic | 50.0% | |
| TCCAAAGACCTTGTGCACGG+CGG | + | chr5_4:24106274-24106293 | None:intergenic | 55.0% | |
| !! | GTGCACAAGGTCTTTGGAGC+TGG | - | chr5_4:24106276-24106295 | Msa0845170:intron | 55.0% |
| ! | AGCTTTTGCCGCCGTGCACA+AGG | - | chr5_4:24106263-24106282 | Msa0845170:intron | 60.0% |
| GCCGCCGTGCACAAGGTCTT+TGG | - | chr5_4:24106270-24106289 | Msa0845170:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 24105990 | 24106334 | 24105990 | ID=Msa0845170;Name=Msa0845170 |
| chr5_4 | mRNA | 24105990 | 24106334 | 24105990 | ID=Msa0845170-mRNA-1;Parent=Msa0845170;Name=Msa0845170-mRNA-1;_AED=0.50;_eAED=0.71;_QI=0|0|0|0.5|0|0|2|0|60 |
| chr5_4 | exon | 24106326 | 24106334 | 24106326 | ID=Msa0845170-mRNA-1:exon:9792;Parent=Msa0845170-mRNA-1 |
| chr5_4 | exon | 24105990 | 24106163 | 24105990 | ID=Msa0845170-mRNA-1:exon:9791;Parent=Msa0845170-mRNA-1 |
| chr5_4 | CDS | 24106326 | 24106334 | 24106326 | ID=Msa0845170-mRNA-1:cds;Parent=Msa0845170-mRNA-1 |
| chr5_4 | CDS | 24105990 | 24106163 | 24105990 | ID=Msa0845170-mRNA-1:cds;Parent=Msa0845170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
