AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0845720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0845720 MtrunA17_Chr5g0417281 89.354 526 54 2 1 526 1 524 0.0 952
Msa0845720 MtrunA17_Chr5g0436761 53.592 515 176 7 1 510 1 457 1.72e-175 502
Msa0845720 MtrunA17_Chr5g0417211 77.287 317 66 3 1 313 1 315 1.69e-174 498
Msa0845720 MtrunA17_Chr5g0417211 60.000 55 21 1 464 517 352 406 6.50e-11 64.3
Msa0845720 MtrunA17_Chr5g0424931 49.548 553 212 10 1 518 8 528 2.18e-163 474
Msa0845720 MtrunA17_Chr2g0289221 70.159 315 89 4 1 312 9 321 8.36e-151 436
Msa0845720 MtrunA17_Chr2g0277461 46.628 519 195 11 4 514 5 449 7.98e-133 393
Msa0845720 MtrunA17_Chr3g0115271 41.115 574 264 13 2 521 9 562 1.00e-123 375
Msa0845720 MtrunA17_Chr1g0178011 50.685 365 163 9 1 354 1 359 1.86e-110 336
Msa0845720 MtrunA17_Chr1g0178011 48.718 78 40 0 426 503 359 436 4.96e-13 71.2
Msa0845720 MtrunA17_Chr5g0424741 40.971 515 230 13 1 448 1 508 3.27e-103 320
Msa0845720 MtrunA17_Chr5g0424801 47.989 348 152 9 1 332 1 335 5.49e-99 304
Msa0845720 MtrunA17_Chr5g0425261 48.276 348 151 9 1 332 1 335 1.18e-97 300
Msa0845720 MtrunA17_Chr2g0326761 35.870 552 254 16 9 494 8 525 3.49e-90 291
Msa0845720 MtrunA17_Chr4g0028971 37.083 480 207 12 1 414 1 451 2.26e-87 283
Msa0845720 MtrunA17_Chr2g0277531 46.006 313 155 10 6 312 14 318 1.15e-85 267
Msa0845720 MtrunA17_Chr1g0181211 39.518 415 180 9 2 353 16 422 2.90e-83 272
Msa0845720 MtrunA17_Chr1g0199681 38.557 402 174 9 9 351 91 478 5.24e-79 261
Msa0845720 MtrunA17_Chr7g0264171 33.512 561 266 18 9 495 28 555 7.20e-75 248
Msa0845720 MtrunA17_Chr5g0425661 53.093 194 87 2 12 204 37 227 4.19e-65 211
Msa0845720 MtrunA17_Chr7g0264161 38.907 311 159 8 22 312 2 301 6.93e-64 210
Msa0845720 MtrunA17_Chr4g0009751 33.854 384 201 9 12 359 19 385 7.89e-60 208
Msa0845720 MtrunA17_Chr2g0282961 34.493 345 192 8 12 332 53 387 3.77e-59 206
Msa0845720 MtrunA17_Chr4g0046371 34.091 352 188 9 12 332 38 376 2.67e-58 205
Msa0845720 MtrunA17_Chr2g0276781 36.034 358 206 8 12 353 23 373 3.77e-58 204
Msa0845720 MtrunA17_Chr8g0346711 35.659 387 209 12 2 360 14 388 1.51e-57 203
Msa0845720 MtrunA17_Chr7g0239061 34.375 352 205 8 1 334 3 346 1.98e-57 202
Msa0845720 MtrunA17_Chr2g0285461 34.545 385 215 12 9 368 20 392 8.46e-57 204
Msa0845720 MtrunA17_Chr5g0433301 33.509 379 219 11 12 367 24 392 3.84e-55 198
Msa0845720 MtrunA17_Chr8g0376771 33.862 378 226 10 9 368 20 391 4.96e-55 199
Msa0845720 MtrunA17_Chr8g0385791 34.254 362 212 8 12 354 59 413 1.48e-54 197
Msa0845720 MtrunA17_Chr1g0156321 32.553 470 263 17 12 448 43 491 1.72e-54 197
Msa0845720 MtrunA17_Chr2g0301251 34.085 355 200 7 9 340 20 363 1.24e-53 196
Msa0845720 MtrunA17_Chr3g0109391 33.161 386 211 12 12 367 26 394 2.33e-53 193
Msa0845720 MtrunA17_Chr4g0070181 34.000 350 211 6 9 342 20 365 4.21e-53 194
Msa0845720 MtrunA17_Chr2g0326281 34.456 386 196 15 12 352 56 429 8.60e-53 192
Msa0845720 MtrunA17_Chr8g0389081 35.311 354 189 10 9 335 29 369 8.60e-52 190
Msa0845720 MtrunA17_Chr4g0029671 34.294 347 198 10 12 332 54 396 1.73e-49 182
Msa0845720 MtrunA17_Chr5g0424791 43.243 148 74 4 63 210 14 151 1.38e-27 108
Msa0845720 MtrunA17_Chr5g0425651 50.000 120 53 5 231 344 2 120 2.71e-26 103
Msa0845720 MtrunA17_Chr8g0356211 27.434 226 148 6 122 335 2 223 7.64e-25 105
Msa0845720 MtrunA17_Chr5g0419861 42.478 113 58 3 53 162 15 123 6.96e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0845720 AT1G77850.1 36.822 535 270 11 9 494 16 531 7.08e-107 331
Msa0845720 AT1G77850.2 36.822 535 270 11 9 494 16 531 8.33e-107 332
Msa0845720 AT2G28350.2 39.655 406 181 9 4 351 2 401 1.16e-84 276
Msa0845720 AT2G28350.1 39.655 406 181 9 4 351 2 401 1.55e-84 276
Msa0845720 AT4G30080.1 36.784 454 217 9 9 411 16 450 1.97e-84 275
Msa0845720 AT4G23980.1 37.982 337 187 8 12 332 12 342 1.18e-63 219
Msa0845720 AT4G23980.2 37.982 337 187 8 12 332 12 342 1.26e-63 219
Msa0845720 AT2G33860.1 36.261 353 185 8 12 337 54 393 1.98e-63 218
Msa0845720 AT2G46530.1 36.111 360 204 10 12 355 21 370 1.88e-58 204
Msa0845720 AT5G37020.2 33.687 377 219 10 12 366 22 389 2.20e-58 207
Msa0845720 AT5G37020.1 33.687 377 219 10 12 366 22 389 3.62e-58 207
Msa0845720 AT2G46530.3 36.111 360 204 10 12 355 42 391 4.27e-58 204
Msa0845720 AT3G61830.1 35.097 359 209 9 12 353 25 376 9.34e-58 202
Msa0845720 AT1G59750.1 34.734 357 212 7 12 352 22 373 1.00e-56 201
Msa0845720 AT1G59750.3 34.734 357 212 7 12 352 22 373 1.00e-56 201
Msa0845720 AT1G59750.2 34.734 357 212 7 12 352 22 373 1.02e-56 201
Msa0845720 AT1G59750.4 34.734 357 212 7 12 352 22 373 1.11e-56 201
Msa0845720 AT5G60450.2 36.538 364 209 9 7 349 36 398 1.76e-56 202
Msa0845720 AT1G35520.1 35.457 361 205 9 12 355 27 376 2.53e-56 198
Msa0845720 AT1G19850.1 36.011 361 190 12 9 342 51 397 3.58e-56 202
Msa0845720 AT1G19850.2 36.011 361 190 12 9 342 40 386 3.82e-56 202
Msa0845720 AT5G60450.1 36.769 359 205 9 12 349 65 422 4.87e-56 201
Msa0845720 AT1G30330.3 34.711 363 204 11 12 351 25 377 1.74e-54 197
Msa0845720 AT1G30330.2 34.711 363 204 11 12 351 25 377 1.74e-54 197
Msa0845720 AT1G30330.1 34.711 363 204 11 12 351 23 375 2.21e-54 197
Msa0845720 AT5G62000.4 34.332 367 205 10 12 354 61 415 7.52e-54 195
Msa0845720 AT5G62000.2 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0845720 AT5G62000.5 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0845720 AT5G62000.3 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0845720 AT5G62000.1 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0845720 AT1G34410.1 35.693 339 191 8 12 334 27 354 1.02e-53 191
Msa0845720 AT1G35540.1 35.294 340 193 8 12 335 27 355 1.64e-53 191
Msa0845720 AT1G35240.1 35.693 339 191 8 12 334 27 354 3.56e-53 190
Msa0845720 AT1G34390.1 33.973 365 203 11 12 355 27 374 5.76e-53 189
Msa0845720 AT1G34310.1 35.652 345 193 9 7 334 22 354 6.92e-53 189
Msa0845720 AT1G19220.1 33.978 362 195 10 9 342 20 365 1.17e-51 190
Msa0845720 AT5G20730.3 33.333 357 205 7 9 342 21 367 3.72e-50 186
Msa0845720 AT5G20730.1 33.333 357 205 7 9 342 21 367 3.87e-50 186
Msa0845720 AT5G20730.2 33.613 357 203 8 9 342 21 366 1.16e-49 184
Msa0845720 AT2G33860.2 39.076 238 128 4 114 337 2 236 4.90e-49 176
Msa0845720 AT3G61830.2 34.951 309 180 8 59 353 24 325 5.47e-46 169
Msa0845720 AT1G34170.1 30.523 344 204 9 12 334 25 354 1.13e-44 165
Msa0845720 AT5G37020.3 34.328 268 157 7 114 366 31 294 1.76e-42 162
Msa0845720 AT2G46530.2 37.209 258 141 8 111 355 34 283 4.78e-40 152
Msa0845720 AT1G34170.2 30.952 336 187 11 12 334 25 328 1.84e-38 147
Msa0845720 AT1G34170.3 31.250 336 186 11 12 334 25 328 3.84e-38 147
Msa0845720 AT1G43950.1 36.364 209 113 5 12 213 27 222 1.23e-33 127

Find 132 sgRNAs with CRISPR-Local

Find 346 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTTAAACTCTATATT+TGG 0.091140 5_4:+25081491 None:intergenic
CCTGGTTCATTTATGCTATT+TGG 0.118465 5_4:-25081179 Msa0845720:CDS
TCTCAATATCATAGGTTAAA+TGG 0.119810 5_4:-25081709 Msa0845720:intron
GCACAGCACTGCCAGATTTC+TGG 0.173902 5_4:+25083206 None:intergenic
TGCTGCATTCAGTGATAAAA+AGG 0.230634 5_4:-25081558 Msa0845720:CDS
CAATCTTGGTCCTATATCTT+TGG 0.252865 5_4:-25081288 Msa0845720:CDS
GGGAGAGTGTGTTAGGGTTT+GGG 0.258688 5_4:+25083102 None:intergenic
CGTGTTTACTACTTCCCATT+AGG 0.273970 5_4:-25083149 Msa0845720:CDS
CCTAACACACTCTCCCTTCT+TGG 0.290201 5_4:-25083095 Msa0845720:CDS
CCAAGAAGGGAGAGTGTGTT+AGG 0.303749 5_4:+25083095 None:intergenic
GTGATATTCACGGCAAAGTT+TGG 0.308610 5_4:-25082767 Msa0845720:CDS
ATGTGTTCTAAGTGCCCTAA+TGG 0.313804 5_4:+25083134 None:intergenic
TTTGATGCCTTCAATTTCAT+TGG 0.329868 5_4:+25081085 None:intergenic
TGAACCAGGACTAACTTTCT+TGG 0.334964 5_4:+25081193 None:intergenic
GATAAAAGCAATGGTTTCCT+TGG 0.335382 5_4:-25081356 Msa0845720:CDS
TCTGGAACGTGCGAGACTTT+AGG 0.336417 5_4:+25081680 None:intergenic
TAAACACGAGAATATAGTTT+TGG 0.340637 5_4:+25083164 None:intergenic
TGAGTCTTTCATGAAAATTA+AGG 0.342069 5_4:+25082647 None:intergenic
GATATAGGACCAAGATTGTT+TGG 0.344613 5_4:+25081293 None:intergenic
GATTTCTGGCTCAACACGGC+TGG 0.344702 5_4:+25083220 None:intergenic
AGGGAGAGTGTGTTAGGGTT+TGG 0.345088 5_4:+25083101 None:intergenic
ATTGTTCAACAATGTTTGAT+TGG 0.354882 5_4:+25081454 None:intergenic
GTTTACGGAGGCATTGGAAT+TGG 0.359403 5_4:-25082553 Msa0845720:CDS
ATGAATATTAAGTGGAATCT+TGG 0.367936 5_4:-25082438 Msa0845720:CDS
CTCAATATCATAGGTTAAAT+GGG 0.383997 5_4:-25081708 Msa0845720:intron
GCCGTGTTGAGCCAGAAATC+TGG 0.395763 5_4:-25083217 Msa0845720:CDS
GTGTTTACTACTTCCCATTA+GGG 0.402184 5_4:-25083148 Msa0845720:CDS
GAAATCTGGCAGTGCTGTGC+AGG 0.404779 5_4:-25083203 Msa0845720:CDS
AATGTTTAAACTCTATATTT+GGG 0.405482 5_4:+25081492 None:intergenic
AAAGAGATGCTCAAAATTTG+AGG 0.405775 5_4:-25082376 Msa0845720:CDS
GTTCGCCGACAGACAAAGTT+TGG 0.410958 5_4:-25082603 Msa0845720:CDS
AGCATCCGCCATGGACGATT+AGG 0.415227 5_4:+25082330 None:intergenic
CATTGCATCCTCCACAACTT+TGG 0.422061 5_4:+25082458 None:intergenic
TTCCAGAGAGAGTCAGTCCT+TGG 0.427770 5_4:-25081664 Msa0845720:CDS
TCTTGAAAATCGCTTCCAAC+AGG 0.432199 5_4:+25081143 None:intergenic
TCCCAAGGACTGACTCTCTC+TGG 0.440968 5_4:+25081662 None:intergenic
ATTAATTTCGCGCATTCCAC+TGG 0.445870 5_4:+25082843 None:intergenic
TCTCTTGTGACAAAGTTTGC+CGG 0.446910 5_4:-25081233 Msa0845720:CDS
AGACCAAGATGATGGCAAAA+AGG 0.450916 5_4:-25082928 Msa0845720:CDS
GTGTATTATCCGATGGCTAA+AGG 0.458436 5_4:-25082504 Msa0845720:CDS
GGAGAGTGTGTTAGGGTTTG+GGG 0.461223 5_4:+25083103 None:intergenic
GCTCTTCTTGATTGCATGCA+GGG 0.464239 5_4:-25081428 Msa0845720:CDS
GACATCTGCTTACCACTGAT+TGG 0.464434 5_4:-25082710 Msa0845720:CDS
ATAAAAGCAATGGTTTCCTT+GGG 0.470549 5_4:-25081355 Msa0845720:CDS
TCAAGTGAGCCAAACAATCT+TGG 0.472055 5_4:-25081302 Msa0845720:CDS
GGTGACAAGTCCAAAGATAT+AGG 0.472899 5_4:+25081278 None:intergenic
TGCTCTTCTTGATTGCATGC+AGG 0.478114 5_4:-25081429 Msa0845720:CDS
AAAATCGTTTACGGAGGCAT+TGG 0.479022 5_4:-25082559 Msa0845720:CDS
TCCAGAGAGAGTCAGTCCTT+GGG 0.479886 5_4:-25081663 Msa0845720:CDS
AAAGCTTAGAAAATCTGATC+AGG 0.481737 5_4:-25081585 Msa0845720:CDS
TGGGGTGAGAGTCTTGGTAT+AGG 0.489342 5_4:+25082899 None:intergenic
CTCCTTCGTCCCAAATATAG+AGG 0.491263 5_4:-25081531 Msa0845720:CDS
TTGATTGCATGCAGGGAGCC+CGG 0.491918 5_4:-25081421 Msa0845720:CDS
TCTTGGTCTTCACGAACCTC+AGG 0.497877 5_4:+25082942 None:intergenic
AGTGTGTTAGGGTTTGGGGA+TGG 0.498039 5_4:+25083107 None:intergenic
CTCACTCCTGTCACTAATAA+CGG 0.499710 5_4:-25082984 Msa0845720:CDS
TGTGTTCTAAGTGCCCTAAT+GGG 0.500812 5_4:+25083135 None:intergenic
TACGGAGGCATTGGAATTGG+CGG 0.504623 5_4:-25082550 Msa0845720:CDS
CAAGAGAGTTAGAAATATCA+TGG 0.505101 5_4:+25081249 None:intergenic
ATTTCTGGCTCAACACGGCT+GGG 0.505787 5_4:+25083221 None:intergenic
CCGCCATTGTTAGCATCAGA+TGG 0.508974 5_4:+25082879 None:intergenic
TCACGAACCTCAGGAGATTG+AGG 0.509135 5_4:+25082951 None:intergenic
CGCCATTGTTAGCATCAGAT+GGG 0.516466 5_4:+25082880 None:intergenic
GAGACGGTGCATAGAATGAA+TGG 0.520972 5_4:+25083062 None:intergenic
AAAGGCTCCAATGAAATTGA+AGG 0.522242 5_4:-25081092 Msa0845720:CDS
TGAACACCGTTATTAGTGAC+AGG 0.525054 5_4:+25082978 None:intergenic
GGTAAGATCATAAAACCTGT+TGG 0.525681 5_4:-25081158 Msa0845720:CDS
CAGCATTGAGGTCAAGTGGC+GGG 0.528362 5_4:+25082817 None:intergenic
TTTATGAATGATAAAAGCAA+TGG 0.529057 5_4:-25081365 Msa0845720:CDS
CTCTCCATCAGTGATATTCA+CGG 0.529787 5_4:-25082777 Msa0845720:CDS
GCAGTGCTGTGCAGGCAGTT+CGG 0.530524 5_4:-25083195 Msa0845720:CDS
GGTGTTGCTGTTGTCAGTAC+CGG 0.533496 5_4:+25081214 None:intergenic
AATATTAAGTGGAATCTTGG+AGG 0.534264 5_4:-25082435 Msa0845720:CDS
ATCAGATGGGGTGAGAGTCT+TGG 0.534822 5_4:+25082893 None:intergenic
TGAAGTTGTGTATTATCCGA+TGG 0.535100 5_4:-25082511 Msa0845720:CDS
AAGAGATGCTCAAAATTTGA+GGG 0.539733 5_4:-25082375 Msa0845720:CDS
ATGGGAAATGATGATTGTAA+AGG 0.540341 5_4:-25081110 Msa0845720:CDS
GTTCAACAATGTTTGATTGG+AGG 0.545186 5_4:+25081457 None:intergenic
TCGTCCATGGCGGATGCTTG+AGG 0.546119 5_4:-25082325 Msa0845720:intron
TGGATACAGTGCAAATATGT+CGG 0.548922 5_4:+25081625 None:intergenic
AGGATGCAATGAATATTAAG+TGG 0.550257 5_4:-25082446 Msa0845720:CDS
ATGGACGGGAACTGCCAAGA+AGG 0.550509 5_4:+25083081 None:intergenic
CAAGAAGGGAGAGTGTGTTA+GGG 0.557064 5_4:+25083096 None:intergenic
GTTGACAAGAAGAAACTAGT+TGG 0.559606 5_4:-25082678 Msa0845720:CDS
TTTCCTTGGGAACAATTCTA+CGG 0.560425 5_4:-25081342 Msa0845720:CDS
TTTCAAGAAGTAGATATCAT+GGG 0.561761 5_4:-25081128 Msa0845720:CDS
CGGTGCATTCTCCGTGCCAG+TGG 0.572523 5_4:-25082859 Msa0845720:CDS
AGTGGTAAGCAGATGTCGCA+AGG 0.572865 5_4:+25082716 None:intergenic
AGCCTCTATATTTGGGACGA+AGG 0.575856 5_4:+25081529 None:intergenic
GCTGGGTTGCTGAGAAGACA+TGG 0.576060 5_4:+25083238 None:intergenic
GAGTAGGTCAACCGCGGAGA+CGG 0.578786 5_4:+25083046 None:intergenic
TAGTTTGACGAATACTTCAT+CGG 0.580000 5_4:+25083010 None:intergenic
GTTCGTGAAGACCAAGATGA+TGG 0.580300 5_4:-25082936 Msa0845720:CDS
TATCTGCTCCTAATCGTCCA+TGG 0.582308 5_4:-25082338 Msa0845720:CDS
AAAGTTGGAAGAAGTTGATG+TGG 0.582671 5_4:+25081385 None:intergenic
AGGGAGTCCCACGGTAGACA+TGG 0.583730 5_4:+25082736 None:intergenic
TGGACGGGAACTGCCAAGAA+GGG 0.586243 5_4:+25083082 None:intergenic
TGTTGTTGATGCCAAAGTTG+TGG 0.589052 5_4:-25082469 Msa0845720:CDS
TTACACCAACCTTTAGCCAT+CGG 0.591900 5_4:+25082495 None:intergenic
GGAATTGGCGGATAAGAACA+TGG 0.591988 5_4:-25082538 Msa0845720:CDS
TTAGAAATATCATGGCTATG+AGG 0.592683 5_4:+25081257 None:intergenic
AACACCAAGAAAGTTAGTCC+TGG 0.601578 5_4:-25081197 Msa0845720:CDS
CAGCACCAAACTTTGTCTGT+CGG 0.605931 5_4:+25082598 None:intergenic
GAAATAGTTTCAACCTCCCA+AGG 0.609478 5_4:+25081647 None:intergenic
TTTGCCGTGAATATCACTGA+TGG 0.609703 5_4:+25082773 None:intergenic
CCAAATAGCATAAATGAACC+AGG 0.612115 5_4:+25081179 None:intergenic
AGAGAGAGTCAGTCCTTGGG+AGG 0.615146 5_4:-25081660 Msa0845720:CDS
ATATTAAGTGGAATCTTGGA+GGG 0.616357 5_4:-25082434 Msa0845720:CDS
ATTATCCGATGGCTAAAGGT+TGG 0.620292 5_4:-25082500 Msa0845720:CDS
CATTCTATGCACCGTCTCCG+CGG 0.621676 5_4:-25083057 Msa0845720:CDS
TCAGACGGAAATATCTCAGT+CGG 0.627485 5_4:-25082627 Msa0845720:CDS
GACAAGAAGAAACTAGTTGG+CGG 0.628517 5_4:-25082675 Msa0845720:CDS
CGGTGCATAGAATGAATGGA+CGG 0.632419 5_4:+25083066 None:intergenic
TATACCTCAAGCATCCGCCA+TGG 0.633522 5_4:+25082321 None:intergenic
GTGCCGTAGAATTGTTCCCA+AGG 0.635548 5_4:+25081339 None:intergenic
TGTTGATGCCAAAGTTGTGG+AGG 0.640943 5_4:-25082466 Msa0845720:CDS
AACGAACTCACTCCAATCAG+TGG 0.642790 5_4:+25082698 None:intergenic
GGTGCATAGAATGAATGGAC+GGG 0.644683 5_4:+25083067 None:intergenic
CGTTATTAGTGACAGGAGTG+AGG 0.644895 5_4:+25082985 None:intergenic
CGGTACAAGAGTCAGCGAGT+AGG 0.652069 5_4:+25083030 None:intergenic
TTTCGCGCATTCCACTGGCA+CGG 0.656401 5_4:+25082848 None:intergenic
ACCCCATCTGATGCTAACAA+TGG 0.658035 5_4:-25082882 Msa0845720:CDS
CTGATGGAGAGTTCTTGAGA+CGG 0.666946 5_4:+25082789 None:intergenic
GTGGTAAGCAGATGTCGCAA+GGG 0.667574 5_4:+25082717 None:intergenic
TCACGAACCTCAATCTCCTG+AGG 0.668607 5_4:-25082958 Msa0845720:CDS
TCAGCATTGAGGTCAAGTGG+CGG 0.682470 5_4:+25082816 None:intergenic
GTCAGCGAGTAGGTCAACCG+CGG 0.683086 5_4:+25083040 None:intergenic
GCCATTGTTAGCATCAGATG+GGG 0.683328 5_4:+25082881 None:intergenic
GCCAGATTTCTGGCTCAACA+CGG 0.683376 5_4:+25083216 None:intergenic
CCATCTGATGCTAACAATGG+CGG 0.690560 5_4:-25082879 Msa0845720:CDS
GATGTCGCAAGGGAGTCCCA+CGG 0.699974 5_4:+25082727 None:intergenic
CTGCTCCTAATCGTCCATGG+CGG 0.723801 5_4:-25082335 Msa0845720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTAACTTTCTTTTTT+AGG - chr5_4:25082176-25082195 Msa0845720:intron 10.0%
!!! TTAATTAACTTTCTTTTTTA+GGG - chr5_4:25082177-25082196 Msa0845720:intron 10.0%
!!! ATCATTTAAAATATATGATA+AGG + chr5_4:25082552-25082571 None:intergenic 10.0%
!!! TATCATATATTTTAAATGAT+TGG - chr5_4:25082552-25082571 Msa0845720:CDS 10.0%
!!! TTTAATTAACTTTCTTTTTT+AGG - chr5_4:25082176-25082195 Msa0845720:intron 10.0%
!!! TTAATTAACTTTCTTTTTTA+GGG - chr5_4:25082177-25082196 Msa0845720:intron 10.0%
!!! ATCATTTAAAATATATGATA+AGG + chr5_4:25082552-25082571 None:intergenic 10.0%
!!! TATCATATATTTTAAATGAT+TGG - chr5_4:25082552-25082571 Msa0845720:CDS 10.0%
!! AAAAAGAAAGTTAATTAAAC+AGG + chr5_4:25082175-25082194 None:intergenic 15.0%
!!! TATTTTTATCTCAATATCAT+AGG - chr5_4:25082589-25082608 Msa0845720:CDS 15.0%
!! AATGTTTAAACTCTATATTT+GGG + chr5_4:25082817-25082836 None:intergenic 15.0%
!! AAATGTTTAAACTCTATATT+TGG + chr5_4:25082818-25082837 None:intergenic 15.0%
!! AAAAAGAAAGTTAATTAAAC+AGG + chr5_4:25082175-25082194 None:intergenic 15.0%
!!! TATTTTTATCTCAATATCAT+AGG - chr5_4:25082589-25082608 Msa0845720:CDS 15.0%
!! AATGTTTAAACTCTATATTT+GGG + chr5_4:25082817-25082836 None:intergenic 15.0%
!! AAATGTTTAAACTCTATATT+TGG + chr5_4:25082818-25082837 None:intergenic 15.0%
!! TATTTCAACTTCATAAAGAA+TGG + chr5_4:25082279-25082298 None:intergenic 20.0%
!! ATTCTTTATGAAGTTGAAAT+AGG - chr5_4:25082278-25082297 Msa0845720:intron 20.0%
!! TTGTACAGTATACTTTATAT+AGG - chr5_4:25082336-25082355 Msa0845720:CDS 20.0%
!! AAACTTAAATACCATTTCTT+AGG + chr5_4:25082448-25082467 None:intergenic 20.0%
!!! ACATTAAGAGTATTTACAAT+AGG + chr5_4:25082490-25082509 None:intergenic 20.0%
!! AAATACTCTTAATGTTAAGT+AGG - chr5_4:25082495-25082514 Msa0845720:CDS 20.0%
!! TTTATGAATGATAAAAGCAA+TGG - chr5_4:25082941-25082960 Msa0845720:CDS 20.0%
!! TATTTCAACTTCATAAAGAA+TGG + chr5_4:25082279-25082298 None:intergenic 20.0%
!! ATTCTTTATGAAGTTGAAAT+AGG - chr5_4:25082278-25082297 Msa0845720:intron 20.0%
!! TTGTACAGTATACTTTATAT+AGG - chr5_4:25082336-25082355 Msa0845720:CDS 20.0%
!! AAACTTAAATACCATTTCTT+AGG + chr5_4:25082448-25082467 None:intergenic 20.0%
!!! ACATTAAGAGTATTTACAAT+AGG + chr5_4:25082490-25082509 None:intergenic 20.0%
!! AAATACTCTTAATGTTAAGT+AGG - chr5_4:25082495-25082514 Msa0845720:CDS 20.0%
!! TTTATGAATGATAAAAGCAA+TGG - chr5_4:25082941-25082960 Msa0845720:CDS 20.0%
!! TAAACACGAGAATATAGTTT+TGG + chr5_4:25081145-25081164 None:intergenic 25.0%
! TGAGTCTTTCATGAAAATTA+AGG + chr5_4:25081662-25081681 None:intergenic 25.0%
! GATAACAGAAAAATCGTTTA+CGG - chr5_4:25081738-25081757 Msa0845720:intron 25.0%
! ATGAATATTAAGTGGAATCT+TGG - chr5_4:25081868-25081887 Msa0845720:intron 25.0%
! AGTCATTGCAGTTAATTTAA+TGG + chr5_4:25082233-25082252 None:intergenic 25.0%
! TTCTTAGGAAATATCACTAT+TGG + chr5_4:25082433-25082452 None:intergenic 25.0%
! TAGTGATATTTCCTAAGAAA+TGG - chr5_4:25082434-25082453 Msa0845720:CDS 25.0%
! TCTCAATATCATAGGTTAAA+TGG - chr5_4:25082597-25082616 Msa0845720:CDS 25.0%
! CTCAATATCATAGGTTAAAT+GGG - chr5_4:25082598-25082617 Msa0845720:CDS 25.0%
!!! GATTTTCTAAGCTTTTTTGT+CGG + chr5_4:25082716-25082735 None:intergenic 25.0%
! TCAAAAAAGCCTCTATATTT+GGG + chr5_4:25082787-25082806 None:intergenic 25.0%
! TTCAAAAAAGCCTCTATATT+TGG + chr5_4:25082788-25082807 None:intergenic 25.0%
! ATTGTTCAACAATGTTTGAT+TGG + chr5_4:25082855-25082874 None:intergenic 25.0%
!! GCTTTTATCATTCATAAAGT+TGG + chr5_4:25082939-25082958 None:intergenic 25.0%
!!! TTTTCAAGAAGTAGATATCA+TGG - chr5_4:25083177-25083196 Msa0845720:CDS 25.0%
! TTTCAAGAAGTAGATATCAT+GGG - chr5_4:25083178-25083197 Msa0845720:CDS 25.0%
!! TAAACACGAGAATATAGTTT+TGG + chr5_4:25081145-25081164 None:intergenic 25.0%
! TGAGTCTTTCATGAAAATTA+AGG + chr5_4:25081662-25081681 None:intergenic 25.0%
! GATAACAGAAAAATCGTTTA+CGG - chr5_4:25081738-25081757 Msa0845720:intron 25.0%
! ATGAATATTAAGTGGAATCT+TGG - chr5_4:25081868-25081887 Msa0845720:intron 25.0%
! AGTCATTGCAGTTAATTTAA+TGG + chr5_4:25082233-25082252 None:intergenic 25.0%
! TTCTTAGGAAATATCACTAT+TGG + chr5_4:25082433-25082452 None:intergenic 25.0%
! TAGTGATATTTCCTAAGAAA+TGG - chr5_4:25082434-25082453 Msa0845720:CDS 25.0%
! TCTCAATATCATAGGTTAAA+TGG - chr5_4:25082597-25082616 Msa0845720:CDS 25.0%
! CTCAATATCATAGGTTAAAT+GGG - chr5_4:25082598-25082617 Msa0845720:CDS 25.0%
!!! GATTTTCTAAGCTTTTTTGT+CGG + chr5_4:25082716-25082735 None:intergenic 25.0%
! TCAAAAAAGCCTCTATATTT+GGG + chr5_4:25082787-25082806 None:intergenic 25.0%
! TTCAAAAAAGCCTCTATATT+TGG + chr5_4:25082788-25082807 None:intergenic 25.0%
! ATTGTTCAACAATGTTTGAT+TGG + chr5_4:25082855-25082874 None:intergenic 25.0%
!! GCTTTTATCATTCATAAAGT+TGG + chr5_4:25082939-25082958 None:intergenic 25.0%
!!! TTTTCAAGAAGTAGATATCA+TGG - chr5_4:25083177-25083196 Msa0845720:CDS 25.0%
! TTTCAAGAAGTAGATATCAT+GGG - chr5_4:25083178-25083197 Msa0845720:CDS 25.0%
TAGTTTGACGAATACTTCAT+CGG + chr5_4:25081299-25081318 None:intergenic 30.0%
! ATTTTCATGAAAGACTCAGA+CGG - chr5_4:25081664-25081683 Msa0845720:CDS 30.0%
AGGATGCAATGAATATTAAG+TGG - chr5_4:25081860-25081879 Msa0845720:intron 30.0%
AATATTAAGTGGAATCTTGG+AGG - chr5_4:25081871-25081890 Msa0845720:intron 30.0%
ATATTAAGTGGAATCTTGGA+GGG - chr5_4:25081872-25081891 Msa0845720:intron 30.0%
AAAGAGATGCTCAAAATTTG+AGG - chr5_4:25081930-25081949 Msa0845720:intron 30.0%
AAGAGATGCTCAAAATTTGA+GGG - chr5_4:25081931-25081950 Msa0845720:intron 30.0%
CTTGGTCTCATAATTAACTA+GGG + chr5_4:25082080-25082099 None:intergenic 30.0%
ACTTGGTCTCATAATTAACT+AGG + chr5_4:25082081-25082100 None:intergenic 30.0%
TTATGAGACCAAGTAAGTTA+CGG - chr5_4:25082087-25082106 Msa0845720:intron 30.0%
AAGATAAACCGTAACTTACT+TGG + chr5_4:25082098-25082117 None:intergenic 30.0%
CATAAACTGAGACAACTTAT+TGG + chr5_4:25082309-25082328 None:intergenic 30.0%
AAAGCTTAGAAAATCTGATC+AGG - chr5_4:25082721-25082740 Msa0845720:CDS 30.0%
!!! TATAGAGGCTTTTTTGAAGA+TGG - chr5_4:25082790-25082809 Msa0845720:CDS 30.0%
!! AAGTTGATGTGGAAAAAAGA+CGG + chr5_4:25082913-25082932 None:intergenic 30.0%
ATAAAAGCAATGGTTTCCTT+GGG - chr5_4:25082951-25082970 Msa0845720:CDS 30.0%
TTAGAAATATCATGGCTATG+AGG + chr5_4:25083052-25083071 None:intergenic 30.0%
CAAGAGAGTTAGAAATATCA+TGG + chr5_4:25083060-25083079 None:intergenic 30.0%
ATGGGAAATGATGATTGTAA+AGG - chr5_4:25083196-25083215 Msa0845720:CDS 30.0%
TTTGATGCCTTCAATTTCAT+TGG + chr5_4:25083224-25083243 None:intergenic 30.0%
TAGTTTGACGAATACTTCAT+CGG + chr5_4:25081299-25081318 None:intergenic 30.0%
! ATTTTCATGAAAGACTCAGA+CGG - chr5_4:25081664-25081683 Msa0845720:CDS 30.0%
AGGATGCAATGAATATTAAG+TGG - chr5_4:25081860-25081879 Msa0845720:intron 30.0%
AATATTAAGTGGAATCTTGG+AGG - chr5_4:25081871-25081890 Msa0845720:intron 30.0%
ATATTAAGTGGAATCTTGGA+GGG - chr5_4:25081872-25081891 Msa0845720:intron 30.0%
AAAGAGATGCTCAAAATTTG+AGG - chr5_4:25081930-25081949 Msa0845720:intron 30.0%
AAGAGATGCTCAAAATTTGA+GGG - chr5_4:25081931-25081950 Msa0845720:intron 30.0%
CTTGGTCTCATAATTAACTA+GGG + chr5_4:25082080-25082099 None:intergenic 30.0%
ACTTGGTCTCATAATTAACT+AGG + chr5_4:25082081-25082100 None:intergenic 30.0%
TTATGAGACCAAGTAAGTTA+CGG - chr5_4:25082087-25082106 Msa0845720:intron 30.0%
AAGATAAACCGTAACTTACT+TGG + chr5_4:25082098-25082117 None:intergenic 30.0%
CATAAACTGAGACAACTTAT+TGG + chr5_4:25082309-25082328 None:intergenic 30.0%
AAAGCTTAGAAAATCTGATC+AGG - chr5_4:25082721-25082740 Msa0845720:CDS 30.0%
!!! TATAGAGGCTTTTTTGAAGA+TGG - chr5_4:25082790-25082809 Msa0845720:CDS 30.0%
!! AAGTTGATGTGGAAAAAAGA+CGG + chr5_4:25082913-25082932 None:intergenic 30.0%
ATAAAAGCAATGGTTTCCTT+GGG - chr5_4:25082951-25082970 Msa0845720:CDS 30.0%
TTAGAAATATCATGGCTATG+AGG + chr5_4:25083052-25083071 None:intergenic 30.0%
CAAGAGAGTTAGAAATATCA+TGG + chr5_4:25083060-25083079 None:intergenic 30.0%
ATGGGAAATGATGATTGTAA+AGG - chr5_4:25083196-25083215 Msa0845720:CDS 30.0%
TTTGATGCCTTCAATTTCAT+TGG + chr5_4:25083224-25083243 None:intergenic 30.0%
GTGTTTACTACTTCCCATTA+GGG - chr5_4:25081158-25081177 Msa0845720:CDS 35.0%
GTTGACAAGAAGAAACTAGT+TGG - chr5_4:25081628-25081647 Msa0845720:CDS 35.0%
AACAGAAAAATCGTTTACGG+AGG - chr5_4:25081741-25081760 Msa0845720:intron 35.0%
TGAAGTTGTGTATTATCCGA+TGG - chr5_4:25081795-25081814 Msa0845720:intron 35.0%
AACTGAGACAACTTATTGGA+CGG + chr5_4:25082305-25082324 None:intergenic 35.0%
TGGATACAGTGCAAATATGT+CGG + chr5_4:25082684-25082703 None:intergenic 35.0%
!!! TTTTTTGTCGGTTGGAATTG+TGG + chr5_4:25082704-25082723 None:intergenic 35.0%
!!! TTCTAAGCTTTTTTGTCGGT+TGG + chr5_4:25082712-25082731 None:intergenic 35.0%
TGCTGCATTCAGTGATAAAA+AGG - chr5_4:25082748-25082767 Msa0845720:CDS 35.0%
GCTGCATTCAGTGATAAAAA+GGG - chr5_4:25082749-25082768 Msa0845720:CDS 35.0%
GTTCAACAATGTTTGATTGG+AGG + chr5_4:25082852-25082871 None:intergenic 35.0%
!! AAAGTTGGAAGAAGTTGATG+TGG + chr5_4:25082924-25082943 None:intergenic 35.0%
GATAAAAGCAATGGTTTCCT+TGG - chr5_4:25082950-25082969 Msa0845720:CDS 35.0%
TTTCCTTGGGAACAATTCTA+CGG - chr5_4:25082964-25082983 Msa0845720:CDS 35.0%
GATATAGGACCAAGATTGTT+TGG + chr5_4:25083016-25083035 None:intergenic 35.0%
CAATCTTGGTCCTATATCTT+TGG - chr5_4:25083018-25083037 Msa0845720:CDS 35.0%
CCAAATAGCATAAATGAACC+AGG + chr5_4:25083130-25083149 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr5_4:25083127-25083146 Msa0845720:CDS 35.0%
GGTAAGATCATAAAACCTGT+TGG - chr5_4:25083148-25083167 Msa0845720:CDS 35.0%
AAAGGCTCCAATGAAATTGA+AGG - chr5_4:25083214-25083233 Msa0845720:CDS 35.0%
GTGTTTACTACTTCCCATTA+GGG - chr5_4:25081158-25081177 Msa0845720:CDS 35.0%
GTTGACAAGAAGAAACTAGT+TGG - chr5_4:25081628-25081647 Msa0845720:CDS 35.0%
AACAGAAAAATCGTTTACGG+AGG - chr5_4:25081741-25081760 Msa0845720:intron 35.0%
TGAAGTTGTGTATTATCCGA+TGG - chr5_4:25081795-25081814 Msa0845720:intron 35.0%
AACTGAGACAACTTATTGGA+CGG + chr5_4:25082305-25082324 None:intergenic 35.0%
TGGATACAGTGCAAATATGT+CGG + chr5_4:25082684-25082703 None:intergenic 35.0%
!!! TTTTTTGTCGGTTGGAATTG+TGG + chr5_4:25082704-25082723 None:intergenic 35.0%
!!! TTCTAAGCTTTTTTGTCGGT+TGG + chr5_4:25082712-25082731 None:intergenic 35.0%
TGCTGCATTCAGTGATAAAA+AGG - chr5_4:25082748-25082767 Msa0845720:CDS 35.0%
GCTGCATTCAGTGATAAAAA+GGG - chr5_4:25082749-25082768 Msa0845720:CDS 35.0%
GTTCAACAATGTTTGATTGG+AGG + chr5_4:25082852-25082871 None:intergenic 35.0%
!! AAAGTTGGAAGAAGTTGATG+TGG + chr5_4:25082924-25082943 None:intergenic 35.0%
GATAAAAGCAATGGTTTCCT+TGG - chr5_4:25082950-25082969 Msa0845720:CDS 35.0%
TTTCCTTGGGAACAATTCTA+CGG - chr5_4:25082964-25082983 Msa0845720:CDS 35.0%
GATATAGGACCAAGATTGTT+TGG + chr5_4:25083016-25083035 None:intergenic 35.0%
CAATCTTGGTCCTATATCTT+TGG - chr5_4:25083018-25083037 Msa0845720:CDS 35.0%
CCAAATAGCATAAATGAACC+AGG + chr5_4:25083130-25083149 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr5_4:25083127-25083146 Msa0845720:CDS 35.0%
GGTAAGATCATAAAACCTGT+TGG - chr5_4:25083148-25083167 Msa0845720:CDS 35.0%
AAAGGCTCCAATGAAATTGA+AGG - chr5_4:25083214-25083233 Msa0845720:CDS 35.0%
CGTGTTTACTACTTCCCATT+AGG - chr5_4:25081157-25081176 Msa0845720:CDS 40.0%
! TGTGTTCTAAGTGCCCTAAT+GGG + chr5_4:25081174-25081193 None:intergenic 40.0%
! ATGTGTTCTAAGTGCCCTAA+TGG + chr5_4:25081175-25081194 None:intergenic 40.0%
CTCACTCCTGTCACTAATAA+CGG - chr5_4:25081322-25081341 Msa0845720:CDS 40.0%
TGAACACCGTTATTAGTGAC+AGG + chr5_4:25081331-25081350 None:intergenic 40.0%
! AGACCAAGATGATGGCAAAA+AGG - chr5_4:25081378-25081397 Msa0845720:CDS 40.0%
! CAACCTTTTTGCCATCATCT+TGG + chr5_4:25081384-25081403 None:intergenic 40.0%
ATTAATTTCGCGCATTCCAC+TGG + chr5_4:25081466-25081485 None:intergenic 40.0%
!! TTTTCAGCATTGAGGTCAAG+TGG + chr5_4:25081496-25081515 None:intergenic 40.0%
CTCTCCATCAGTGATATTCA+CGG - chr5_4:25081529-25081548 Msa0845720:CDS 40.0%
TTTGCCGTGAATATCACTGA+TGG + chr5_4:25081536-25081555 None:intergenic 40.0%
GTGATATTCACGGCAAAGTT+TGG - chr5_4:25081539-25081558 Msa0845720:CDS 40.0%
GACAAGAAGAAACTAGTTGG+CGG - chr5_4:25081631-25081650 Msa0845720:CDS 40.0%
TCAGACGGAAATATCTCAGT+CGG - chr5_4:25081679-25081698 Msa0845720:CDS 40.0%
! AAAATCGTTTACGGAGGCAT+TGG - chr5_4:25081747-25081766 Msa0845720:intron 40.0%
GTGTATTATCCGATGGCTAA+AGG - chr5_4:25081802-25081821 Msa0845720:intron 40.0%
ATTATCCGATGGCTAAAGGT+TGG - chr5_4:25081806-25081825 Msa0845720:intron 40.0%
TTACACCAACCTTTAGCCAT+CGG + chr5_4:25081814-25081833 None:intergenic 40.0%
TGTTGTTGATGCCAAAGTTG+TGG - chr5_4:25081837-25081856 Msa0845720:intron 40.0%
!! CACTATTGGAGCAAGTTGAT+AGG + chr5_4:25082419-25082438 None:intergenic 40.0%
GAAATAGTTTCAACCTCCCA+AGG + chr5_4:25082662-25082681 None:intergenic 40.0%
TCAAGTGAGCCAAACAATCT+TGG - chr5_4:25083004-25083023 Msa0845720:CDS 40.0%
GGTGACAAGTCCAAAGATAT+AGG + chr5_4:25083031-25083050 None:intergenic 40.0%
TCTCTTGTGACAAAGTTTGC+CGG - chr5_4:25083073-25083092 Msa0845720:CDS 40.0%
AACACCAAGAAAGTTAGTCC+TGG - chr5_4:25083109-25083128 Msa0845720:CDS 40.0%
TGAACCAGGACTAACTTTCT+TGG + chr5_4:25083116-25083135 None:intergenic 40.0%
TCTTGAAAATCGCTTCCAAC+AGG + chr5_4:25083166-25083185 None:intergenic 40.0%
CGTGTTTACTACTTCCCATT+AGG - chr5_4:25081157-25081176 Msa0845720:CDS 40.0%
! TGTGTTCTAAGTGCCCTAAT+GGG + chr5_4:25081174-25081193 None:intergenic 40.0%
! ATGTGTTCTAAGTGCCCTAA+TGG + chr5_4:25081175-25081194 None:intergenic 40.0%
CTCACTCCTGTCACTAATAA+CGG - chr5_4:25081322-25081341 Msa0845720:CDS 40.0%
TGAACACCGTTATTAGTGAC+AGG + chr5_4:25081331-25081350 None:intergenic 40.0%
! AGACCAAGATGATGGCAAAA+AGG - chr5_4:25081378-25081397 Msa0845720:CDS 40.0%
! CAACCTTTTTGCCATCATCT+TGG + chr5_4:25081384-25081403 None:intergenic 40.0%
ATTAATTTCGCGCATTCCAC+TGG + chr5_4:25081466-25081485 None:intergenic 40.0%
!! TTTTCAGCATTGAGGTCAAG+TGG + chr5_4:25081496-25081515 None:intergenic 40.0%
CTCTCCATCAGTGATATTCA+CGG - chr5_4:25081529-25081548 Msa0845720:CDS 40.0%
TTTGCCGTGAATATCACTGA+TGG + chr5_4:25081536-25081555 None:intergenic 40.0%
GTGATATTCACGGCAAAGTT+TGG - chr5_4:25081539-25081558 Msa0845720:CDS 40.0%
GACAAGAAGAAACTAGTTGG+CGG - chr5_4:25081631-25081650 Msa0845720:CDS 40.0%
TCAGACGGAAATATCTCAGT+CGG - chr5_4:25081679-25081698 Msa0845720:CDS 40.0%
! AAAATCGTTTACGGAGGCAT+TGG - chr5_4:25081747-25081766 Msa0845720:intron 40.0%
GTGTATTATCCGATGGCTAA+AGG - chr5_4:25081802-25081821 Msa0845720:intron 40.0%
ATTATCCGATGGCTAAAGGT+TGG - chr5_4:25081806-25081825 Msa0845720:intron 40.0%
TTACACCAACCTTTAGCCAT+CGG + chr5_4:25081814-25081833 None:intergenic 40.0%
TGTTGTTGATGCCAAAGTTG+TGG - chr5_4:25081837-25081856 Msa0845720:intron 40.0%
!! CACTATTGGAGCAAGTTGAT+AGG + chr5_4:25082419-25082438 None:intergenic 40.0%
GAAATAGTTTCAACCTCCCA+AGG + chr5_4:25082662-25082681 None:intergenic 40.0%
TCAAGTGAGCCAAACAATCT+TGG - chr5_4:25083004-25083023 Msa0845720:CDS 40.0%
GGTGACAAGTCCAAAGATAT+AGG + chr5_4:25083031-25083050 None:intergenic 40.0%
TCTCTTGTGACAAAGTTTGC+CGG - chr5_4:25083073-25083092 Msa0845720:CDS 40.0%
AACACCAAGAAAGTTAGTCC+TGG - chr5_4:25083109-25083128 Msa0845720:CDS 40.0%
TGAACCAGGACTAACTTTCT+TGG + chr5_4:25083116-25083135 None:intergenic 40.0%
TCTTGAAAATCGCTTCCAAC+AGG + chr5_4:25083166-25083185 None:intergenic 40.0%
! CAAGAAGGGAGAGTGTGTTA+GGG + chr5_4:25081213-25081232 None:intergenic 45.0%
GGTGCATAGAATGAATGGAC+GGG + chr5_4:25081242-25081261 None:intergenic 45.0%
CGGTGCATAGAATGAATGGA+CGG + chr5_4:25081243-25081262 None:intergenic 45.0%
GAGACGGTGCATAGAATGAA+TGG + chr5_4:25081247-25081266 None:intergenic 45.0%
CGTTATTAGTGACAGGAGTG+AGG + chr5_4:25081324-25081343 None:intergenic 45.0%
GTTCGTGAAGACCAAGATGA+TGG - chr5_4:25081370-25081389 Msa0845720:CDS 45.0%
ACCCCATCTGATGCTAACAA+TGG - chr5_4:25081424-25081443 Msa0845720:CDS 45.0%
GCCATTGTTAGCATCAGATG+GGG + chr5_4:25081428-25081447 None:intergenic 45.0%
CGCCATTGTTAGCATCAGAT+GGG + chr5_4:25081429-25081448 None:intergenic 45.0%
CCATCTGATGCTAACAATGG+CGG - chr5_4:25081427-25081446 Msa0845720:CDS 45.0%
CTGATGGAGAGTTCTTGAGA+CGG + chr5_4:25081520-25081539 None:intergenic 45.0%
! AATTTTCGCCATGTCTACCG+TGG - chr5_4:25081562-25081581 Msa0845720:CDS 45.0%
! ATTTTCGCCATGTCTACCGT+GGG - chr5_4:25081563-25081582 Msa0845720:CDS 45.0%
GACATCTGCTTACCACTGAT+TGG - chr5_4:25081596-25081615 Msa0845720:CDS 45.0%
AACGAACTCACTCCAATCAG+TGG + chr5_4:25081611-25081630 None:intergenic 45.0%
CAGCACCAAACTTTGTCTGT+CGG + chr5_4:25081711-25081730 None:intergenic 45.0%
!! GTTTACGGAGGCATTGGAAT+TGG - chr5_4:25081753-25081772 Msa0845720:intron 45.0%
! GGAATTGGCGGATAAGAACA+TGG - chr5_4:25081768-25081787 Msa0845720:intron 45.0%
TGTTGATGCCAAAGTTGTGG+AGG - chr5_4:25081840-25081859 Msa0845720:intron 45.0%
CATTGCATCCTCCACAACTT+TGG + chr5_4:25081851-25081870 None:intergenic 45.0%
TATCTGCTCCTAATCGTCCA+TGG - chr5_4:25081968-25081987 Msa0845720:intron 45.0%
CTCCTTCGTCCCAAATATAG+AGG - chr5_4:25082775-25082794 Msa0845720:CDS 45.0%
! AGCCTCTATATTTGGGACGA+AGG + chr5_4:25082780-25082799 None:intergenic 45.0%
TGCTCTTCTTGATTGCATGC+AGG - chr5_4:25082877-25082896 Msa0845720:CDS 45.0%
GCTCTTCTTGATTGCATGCA+GGG - chr5_4:25082878-25082897 Msa0845720:CDS 45.0%
ATGTGGAAAAAAGACGGTGC+CGG + chr5_4:25082907-25082926 None:intergenic 45.0%
! CAAGAAGGGAGAGTGTGTTA+GGG + chr5_4:25081213-25081232 None:intergenic 45.0%
GGTGCATAGAATGAATGGAC+GGG + chr5_4:25081242-25081261 None:intergenic 45.0%
CGGTGCATAGAATGAATGGA+CGG + chr5_4:25081243-25081262 None:intergenic 45.0%
GAGACGGTGCATAGAATGAA+TGG + chr5_4:25081247-25081266 None:intergenic 45.0%
CGTTATTAGTGACAGGAGTG+AGG + chr5_4:25081324-25081343 None:intergenic 45.0%
GTTCGTGAAGACCAAGATGA+TGG - chr5_4:25081370-25081389 Msa0845720:CDS 45.0%
ACCCCATCTGATGCTAACAA+TGG - chr5_4:25081424-25081443 Msa0845720:CDS 45.0%
GCCATTGTTAGCATCAGATG+GGG + chr5_4:25081428-25081447 None:intergenic 45.0%
CGCCATTGTTAGCATCAGAT+GGG + chr5_4:25081429-25081448 None:intergenic 45.0%
CCATCTGATGCTAACAATGG+CGG - chr5_4:25081427-25081446 Msa0845720:CDS 45.0%
CTGATGGAGAGTTCTTGAGA+CGG + chr5_4:25081520-25081539 None:intergenic 45.0%
! AATTTTCGCCATGTCTACCG+TGG - chr5_4:25081562-25081581 Msa0845720:CDS 45.0%
! ATTTTCGCCATGTCTACCGT+GGG - chr5_4:25081563-25081582 Msa0845720:CDS 45.0%
GACATCTGCTTACCACTGAT+TGG - chr5_4:25081596-25081615 Msa0845720:CDS 45.0%
AACGAACTCACTCCAATCAG+TGG + chr5_4:25081611-25081630 None:intergenic 45.0%
CAGCACCAAACTTTGTCTGT+CGG + chr5_4:25081711-25081730 None:intergenic 45.0%
!! GTTTACGGAGGCATTGGAAT+TGG - chr5_4:25081753-25081772 Msa0845720:intron 45.0%
! GGAATTGGCGGATAAGAACA+TGG - chr5_4:25081768-25081787 Msa0845720:intron 45.0%
TGTTGATGCCAAAGTTGTGG+AGG - chr5_4:25081840-25081859 Msa0845720:intron 45.0%
CATTGCATCCTCCACAACTT+TGG + chr5_4:25081851-25081870 None:intergenic 45.0%
TATCTGCTCCTAATCGTCCA+TGG - chr5_4:25081968-25081987 Msa0845720:intron 45.0%
CTCCTTCGTCCCAAATATAG+AGG - chr5_4:25082775-25082794 Msa0845720:CDS 45.0%
! AGCCTCTATATTTGGGACGA+AGG + chr5_4:25082780-25082799 None:intergenic 45.0%
TGCTCTTCTTGATTGCATGC+AGG - chr5_4:25082877-25082896 Msa0845720:CDS 45.0%
GCTCTTCTTGATTGCATGCA+GGG - chr5_4:25082878-25082897 Msa0845720:CDS 45.0%
ATGTGGAAAAAAGACGGTGC+CGG + chr5_4:25082907-25082926 None:intergenic 45.0%
!! ATTTCTGGCTCAACACGGCT+GGG + chr5_4:25081088-25081107 None:intergenic 50.0%
!! GCCAGATTTCTGGCTCAACA+CGG + chr5_4:25081093-25081112 None:intergenic 50.0%
AGTGTGTTAGGGTTTGGGGA+TGG + chr5_4:25081202-25081221 None:intergenic 50.0%
! GGAGAGTGTGTTAGGGTTTG+GGG + chr5_4:25081206-25081225 None:intergenic 50.0%
! GGGAGAGTGTGTTAGGGTTT+GGG + chr5_4:25081207-25081226 None:intergenic 50.0%
! AGGGAGAGTGTGTTAGGGTT+TGG + chr5_4:25081208-25081227 None:intergenic 50.0%
! CCAAGAAGGGAGAGTGTGTT+AGG + chr5_4:25081214-25081233 None:intergenic 50.0%
CCTAACACACTCTCCCTTCT+TGG - chr5_4:25081211-25081230 Msa0845720:CDS 50.0%
TCACGAACCTCAATCTCCTG+AGG - chr5_4:25081348-25081367 Msa0845720:CDS 50.0%
TCACGAACCTCAGGAGATTG+AGG + chr5_4:25081358-25081377 None:intergenic 50.0%
! TCTTGGTCTTCACGAACCTC+AGG + chr5_4:25081367-25081386 None:intergenic 50.0%
TGGGGTGAGAGTCTTGGTAT+AGG + chr5_4:25081410-25081429 None:intergenic 50.0%
ATCAGATGGGGTGAGAGTCT+TGG + chr5_4:25081416-25081435 None:intergenic 50.0%
CCGCCATTGTTAGCATCAGA+TGG + chr5_4:25081430-25081449 None:intergenic 50.0%
! TCAGCATTGAGGTCAAGTGG+CGG + chr5_4:25081493-25081512 None:intergenic 50.0%
! GAGACGGCTTTTCAGCATTG+AGG + chr5_4:25081504-25081523 None:intergenic 50.0%
GTGGTAAGCAGATGTCGCAA+GGG + chr5_4:25081592-25081611 None:intergenic 50.0%
AGTGGTAAGCAGATGTCGCA+AGG + chr5_4:25081593-25081612 None:intergenic 50.0%
GTTCGCCGACAGACAAAGTT+TGG - chr5_4:25081703-25081722 Msa0845720:intron 50.0%
!! TACGGAGGCATTGGAATTGG+CGG - chr5_4:25081756-25081775 Msa0845720:intron 50.0%
TATACCTCAAGCATCCGCCA+TGG + chr5_4:25081988-25082007 None:intergenic 50.0%
! TCTGGAACGTGCGAGACTTT+AGG + chr5_4:25082629-25082648 None:intergenic 50.0%
TTCCAGAGAGAGTCAGTCCT+TGG - chr5_4:25082642-25082661 Msa0845720:CDS 50.0%
TCCAGAGAGAGTCAGTCCTT+GGG - chr5_4:25082643-25082662 Msa0845720:CDS 50.0%
TGTGGAAAAAAGACGGTGCC+GGG + chr5_4:25082906-25082925 None:intergenic 50.0%
GTGCCGTAGAATTGTTCCCA+AGG + chr5_4:25082970-25082989 None:intergenic 50.0%
!! GGTGTTGCTGTTGTCAGTAC+CGG + chr5_4:25083095-25083114 None:intergenic 50.0%
!! ATTTCTGGCTCAACACGGCT+GGG + chr5_4:25081088-25081107 None:intergenic 50.0%
!! GCCAGATTTCTGGCTCAACA+CGG + chr5_4:25081093-25081112 None:intergenic 50.0%
AGTGTGTTAGGGTTTGGGGA+TGG + chr5_4:25081202-25081221 None:intergenic 50.0%
! GGAGAGTGTGTTAGGGTTTG+GGG + chr5_4:25081206-25081225 None:intergenic 50.0%
! GGGAGAGTGTGTTAGGGTTT+GGG + chr5_4:25081207-25081226 None:intergenic 50.0%
! AGGGAGAGTGTGTTAGGGTT+TGG + chr5_4:25081208-25081227 None:intergenic 50.0%
! CCAAGAAGGGAGAGTGTGTT+AGG + chr5_4:25081214-25081233 None:intergenic 50.0%
CCTAACACACTCTCCCTTCT+TGG - chr5_4:25081211-25081230 Msa0845720:CDS 50.0%
TCACGAACCTCAATCTCCTG+AGG - chr5_4:25081348-25081367 Msa0845720:CDS 50.0%
TCACGAACCTCAGGAGATTG+AGG + chr5_4:25081358-25081377 None:intergenic 50.0%
! TCTTGGTCTTCACGAACCTC+AGG + chr5_4:25081367-25081386 None:intergenic 50.0%
TGGGGTGAGAGTCTTGGTAT+AGG + chr5_4:25081410-25081429 None:intergenic 50.0%
ATCAGATGGGGTGAGAGTCT+TGG + chr5_4:25081416-25081435 None:intergenic 50.0%
CCGCCATTGTTAGCATCAGA+TGG + chr5_4:25081430-25081449 None:intergenic 50.0%
! TCAGCATTGAGGTCAAGTGG+CGG + chr5_4:25081493-25081512 None:intergenic 50.0%
! GAGACGGCTTTTCAGCATTG+AGG + chr5_4:25081504-25081523 None:intergenic 50.0%
GTGGTAAGCAGATGTCGCAA+GGG + chr5_4:25081592-25081611 None:intergenic 50.0%
AGTGGTAAGCAGATGTCGCA+AGG + chr5_4:25081593-25081612 None:intergenic 50.0%
GTTCGCCGACAGACAAAGTT+TGG - chr5_4:25081703-25081722 Msa0845720:intron 50.0%
!! TACGGAGGCATTGGAATTGG+CGG - chr5_4:25081756-25081775 Msa0845720:intron 50.0%
TATACCTCAAGCATCCGCCA+TGG + chr5_4:25081988-25082007 None:intergenic 50.0%
! TCTGGAACGTGCGAGACTTT+AGG + chr5_4:25082629-25082648 None:intergenic 50.0%
TTCCAGAGAGAGTCAGTCCT+TGG - chr5_4:25082642-25082661 Msa0845720:CDS 50.0%
TCCAGAGAGAGTCAGTCCTT+GGG - chr5_4:25082643-25082662 Msa0845720:CDS 50.0%
TGTGGAAAAAAGACGGTGCC+GGG + chr5_4:25082906-25082925 None:intergenic 50.0%
GTGCCGTAGAATTGTTCCCA+AGG + chr5_4:25082970-25082989 None:intergenic 50.0%
!! GGTGTTGCTGTTGTCAGTAC+CGG + chr5_4:25083095-25083114 None:intergenic 50.0%
!! GATTTCTGGCTCAACACGGC+TGG + chr5_4:25081089-25081108 None:intergenic 55.0%
GCCGTGTTGAGCCAGAAATC+TGG - chr5_4:25081089-25081108 Msa0845720:CDS 55.0%
GCACAGCACTGCCAGATTTC+TGG + chr5_4:25081103-25081122 None:intergenic 55.0%
GAAATCTGGCAGTGCTGTGC+AGG - chr5_4:25081103-25081122 Msa0845720:CDS 55.0%
TGGACGGGAACTGCCAAGAA+GGG + chr5_4:25081227-25081246 None:intergenic 55.0%
ATGGACGGGAACTGCCAAGA+AGG + chr5_4:25081228-25081247 None:intergenic 55.0%
CATTCTATGCACCGTCTCCG+CGG - chr5_4:25081249-25081268 Msa0845720:CDS 55.0%
CGGTACAAGAGTCAGCGAGT+AGG + chr5_4:25081279-25081298 None:intergenic 55.0%
TTTCGCGCATTCCACTGGCA+CGG + chr5_4:25081461-25081480 None:intergenic 55.0%
! CAGCATTGAGGTCAAGTGGC+GGG + chr5_4:25081492-25081511 None:intergenic 55.0%
CTGCTCCTAATCGTCCATGG+CGG - chr5_4:25081971-25081990 Msa0845720:intron 55.0%
AGCATCCGCCATGGACGATT+AGG + chr5_4:25081979-25081998 None:intergenic 55.0%
! TCCCAAGGACTGACTCTCTC+TGG + chr5_4:25082647-25082666 None:intergenic 55.0%
AGAGAGAGTCAGTCCTTGGG+AGG - chr5_4:25082646-25082665 Msa0845720:CDS 55.0%
TTGATTGCATGCAGGGAGCC+CGG - chr5_4:25082885-25082904 Msa0845720:CDS 55.0%
!! GATTTCTGGCTCAACACGGC+TGG + chr5_4:25081089-25081108 None:intergenic 55.0%
GCCGTGTTGAGCCAGAAATC+TGG - chr5_4:25081089-25081108 Msa0845720:CDS 55.0%
GCACAGCACTGCCAGATTTC+TGG + chr5_4:25081103-25081122 None:intergenic 55.0%
GAAATCTGGCAGTGCTGTGC+AGG - chr5_4:25081103-25081122 Msa0845720:CDS 55.0%
TGGACGGGAACTGCCAAGAA+GGG + chr5_4:25081227-25081246 None:intergenic 55.0%
ATGGACGGGAACTGCCAAGA+AGG + chr5_4:25081228-25081247 None:intergenic 55.0%
CATTCTATGCACCGTCTCCG+CGG - chr5_4:25081249-25081268 Msa0845720:CDS 55.0%
CGGTACAAGAGTCAGCGAGT+AGG + chr5_4:25081279-25081298 None:intergenic 55.0%
TTTCGCGCATTCCACTGGCA+CGG + chr5_4:25081461-25081480 None:intergenic 55.0%
! CAGCATTGAGGTCAAGTGGC+GGG + chr5_4:25081492-25081511 None:intergenic 55.0%
CTGCTCCTAATCGTCCATGG+CGG - chr5_4:25081971-25081990 Msa0845720:intron 55.0%
AGCATCCGCCATGGACGATT+AGG + chr5_4:25081979-25081998 None:intergenic 55.0%
! TCCCAAGGACTGACTCTCTC+TGG + chr5_4:25082647-25082666 None:intergenic 55.0%
AGAGAGAGTCAGTCCTTGGG+AGG - chr5_4:25082646-25082665 Msa0845720:CDS 55.0%
TTGATTGCATGCAGGGAGCC+CGG - chr5_4:25082885-25082904 Msa0845720:CDS 55.0%
GCAGTGCTGTGCAGGCAGTT+CGG - chr5_4:25081111-25081130 Msa0845720:CDS 60.0%
GAGTAGGTCAACCGCGGAGA+CGG + chr5_4:25081263-25081282 None:intergenic 60.0%
GTCAGCGAGTAGGTCAACCG+CGG + chr5_4:25081269-25081288 None:intergenic 60.0%
AGGGAGTCCCACGGTAGACA+TGG + chr5_4:25081573-25081592 None:intergenic 60.0%
GATGTCGCAAGGGAGTCCCA+CGG + chr5_4:25081582-25081601 None:intergenic 60.0%
!! TCGTCCATGGCGGATGCTTG+AGG - chr5_4:25081981-25082000 Msa0845720:intron 60.0%
GCAGTGCTGTGCAGGCAGTT+CGG - chr5_4:25081111-25081130 Msa0845720:CDS 60.0%
GAGTAGGTCAACCGCGGAGA+CGG + chr5_4:25081263-25081282 None:intergenic 60.0%
GTCAGCGAGTAGGTCAACCG+CGG + chr5_4:25081269-25081288 None:intergenic 60.0%
AGGGAGTCCCACGGTAGACA+TGG + chr5_4:25081573-25081592 None:intergenic 60.0%
GATGTCGCAAGGGAGTCCCA+CGG + chr5_4:25081582-25081601 None:intergenic 60.0%
!! TCGTCCATGGCGGATGCTTG+AGG - chr5_4:25081981-25082000 Msa0845720:intron 60.0%
CGGTGCATTCTCCGTGCCAG+TGG - chr5_4:25081447-25081466 Msa0845720:CDS 65.0%
CGGTGCATTCTCCGTGCCAG+TGG - chr5_4:25081447-25081466 Msa0845720:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 25081070 25083258 25081070 ID=Msa0845720;Name=Msa0845720
chr5_4 mRNA 25081070 25083258 25081070 ID=Msa0845720-mRNA-1;Parent=Msa0845720;Name=Msa0845720-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|526
chr5_4 exon 25082326 25083258 25082326 ID=Msa0845720-mRNA-1:exon:10001;Parent=Msa0845720-mRNA-1
chr5_4 exon 25081070 25081717 25081070 ID=Msa0845720-mRNA-1:exon:10000;Parent=Msa0845720-mRNA-1
chr5_4 CDS 25082326 25083258 25082326 ID=Msa0845720-mRNA-1:cds;Parent=Msa0845720-mRNA-1
chr5_4 CDS 25081070 25081717 25081070 ID=Msa0845720-mRNA-1:cds;Parent=Msa0845720-mRNA-1
Gene Sequence

>Msa0845720

ATGTCTTCTCAGCAACCCAGCCGTGTTGAGCCAGAAATCTGGCAGTGCTGTGCAGGCAGTTCGGTTAAAATTCCAAAACTATATTCTCGTGTTTACTACTTCCCATTAGGGCACTTAGAACACATATGTCCATCCCCAAACCCTAACACACTCTCCCTTCTTGGCAGTTCCCGTCCATTCATTCTATGCACCGTCTCCGCGGTTGACCTACTCGCTGACTCTTGTACCGATGAAGTATTCGTCAAACTACTCCTCACTCCTGTCACTAATAACGGTGTTCACGAACCTCAATCTCCTGAGGTTCGTGAAGACCAAGATGATGGCAAAAAGGTTGTTTCCTATACCAAGACTCTCACCCCATCTGATGCTAACAATGGCGGTGCATTCTCCGTGCCAGTGGAATGCGCGAAATTAATCTTCCCGCCACTTGACCTCAATGCTGAAAAGCCGTCTCAAGAACTCTCCATCAGTGATATTCACGGCAAAGTTTGGAATTTTCGCCATGTCTACCGTGGGACTCCCTTGCGACATCTGCTTACCACTGATTGGAGTGAGTTCGTTGACAAGAAGAAACTAGTTGGCGGCGATTCCTTAATTTTCATGAAAGACTCAGACGGAAATATCTCAGTCGGAGTTCGCCGACAGACAAAGTTTGGTGCTGCTGCTAAGATAACAGAAAAATCGTTTACGGAGGCATTGGAATTGGCGGATAAGAACATGGCTTTTGAAGTTGTGTATTATCCGATGGCTAAAGGTTGGTGTAATTTTGTTGTTGATGCCAAAGTTGTGGAGGATGCAATGAATATTAAGTGGAATCTTGGAGGGAGAATTGAACTTTCTTTGAAGAATTTCGATTCTTCAAAGAGATGCTCAAAATTTGAGGGAACAATATCTGCTCTATCTGCTCCTAATCGTCCATGGCGGATGCTTGAGGTTAAATGGGATGAACCTAAAGTCTCGCACGTTCCAGAGAGAGTCAGTCCTTGGGAGGTTGAAACTATTTCCGACATATTTGCACTGTATCCACAATTCCAACCGACAAAAAAGCTTAGAAAATCTGATCAGGATTCTGCTGCATTCAGTGATAAAAAGGGAGACTCCTTCGTCCCAAATATAGAGGCTTTTTTGAAGATGGTCCCAAATATAGAGTTTAAACATTTCGATATGACATCCTCCAATCAAACATTGTTGAACAATGATGCTCTTCTTGATTGCATGCAGGGAGCCCGGCACCGTCTTTTTTCCACATCAACTTCTTCCAACTTTATGAATGATAAAAGCAATGGTTTCCTTGGGAACAATTCTACGGCACTGTTGAGTAATGTGTCAAGTGAGCCAAACAATCTTGGTCCTATATCTTTGGACTTGTCACCTCATAGCCATGATATTTCTAACTCTCTTGTGACAAAGTTTGCCGGTACTGACAACAGCAACACCAAGAAAGTTAGTCCTGGTTCATTTATGCTATTTGGTAAGATCATAAAACCTGTTGGAAGCGATTTTCAAGAAGTAGATATCATGGGAAATGATGATTGTAAAGGCTCCAATGAAATTGAAGGCATCAAACTTGCACAAAAATAG
Protein sequence

>Msa0845720

MSSQQPSRVEPEIWQCCAGSSVKIPKLYSRVYYFPLGHLEHICPSPNPNTLSLLGSSRPFILCTVSAVDLLADSCTDEVFVKLLLTPVTNNGVHEPQSPEVREDQDDGKKVVSYTKTLTPSDANNGGAFSVPVECAKLIFPPLDLNAEKPSQELSISDIHGKVWNFRHVYRGTPLRHLLTTDWSEFVDKKKLVGGDSLIFMKDSDGNISVGVRRQTKFGAAAKITEKSFTEALELADKNMAFEVVYYPMAKGWCNFVVDAKVVEDAMNIKWNLGGRIELSLKNFDSSKRCSKFEGTISALSAPNRPWRMLEVKWDEPKVSHVPERVSPWEVETISDIFALYPQFQPTKKLRKSDQDSAAFSDKKGDSFVPNIEAFLKMVPNIEFKHFDMTSSNQTLLNNDALLDCMQGARHRLFSTSTSSNFMNDKSNGFLGNNSTALLSNVSSEPNNLGPISLDLSPHSHDISNSLVTKFAGTDNSNTKKVSPGSFMLFGKIIKPVGSDFQEVDIMGNDDCKGSNEIEGIKLAQK*