AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0846920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0846920 MtrunA17_Chr8g0392301 59.873 157 39 5 12 146 765 919 2.18e-48 166
Msa0846920 MtrunA17_Chr3g0122391 54.082 98 39 2 53 146 862 957 3.26e-25 100
Msa0846920 MtrunA17_Chr8g0377481 51.546 97 42 2 53 146 805 899 1.93e-22 92.0
Msa0846920 MtrunA17_Chr5g0447641 41.549 142 57 5 28 146 994 1132 5.82e-22 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0846920 AT2G01830.2 50.714 140 46 4 28 146 866 1003 5.21e-34 125
Msa0846920 AT2G01830.1 50.714 140 46 4 28 146 843 980 5.51e-34 125
Msa0846920 AT2G01830.4 50.714 140 46 4 28 146 843 980 5.51e-34 125
Msa0846920 AT2G01830.6 50.714 140 46 4 28 146 843 980 5.51e-34 125
Msa0846920 AT2G01830.3 50.714 140 46 4 28 146 843 980 5.51e-34 125
Msa0846920 AT2G01830.5 50.714 140 46 4 28 146 855 992 7.17e-34 125
Msa0846920 AT1G27320.1 46.377 138 52 4 29 146 813 948 2.77e-26 103
Msa0846920 AT5G35750.1 44.138 145 61 4 20 146 951 1093 1.38e-25 101

Find 29 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTGCTAGAAGAATCATTT+TGG 0.207805 5_4:-27761168 None:intergenic
AAATTCAGTGCTGTTAATAT+TGG 0.260654 5_4:-27761189 None:intergenic
GCTTGCCTTCGGCAAGTTCT+TGG 0.268331 5_4:+27761233 Msa0846920:CDS
TAAATTTGATGCTTGCTTCA+TGG 0.287393 5_4:+27761460 Msa0846920:CDS
CTGCAGCTGCAGCTACCTTC+TGG 0.311196 5_4:-27761358 None:intergenic
TTAACAGCACTGAATTTGAT+AGG 0.313476 5_4:+27761195 Msa0846920:CDS
GTCCTCAACCATGACAATAA+TGG 0.330041 5_4:-27761083 None:intergenic
TGAATTTGATAGGGCTAGTA+TGG 0.357246 5_4:+27761205 Msa0846920:CDS
CTTCGGCAAGTTCTTGGAAT+GGG 0.359478 5_4:+27761239 Msa0846920:CDS
TCCTCAACCATGACAATAAT+GGG 0.374360 5_4:-27761082 None:intergenic
TCTCCATAGCAAGAAAATAT+TGG 0.389020 5_4:+27761313 Msa0846920:CDS
GGACATTCAAATGTTAGAAA+TGG 0.392857 5_4:+27761481 Msa0846920:CDS
CCTTCGGCAAGTTCTTGGAA+TGG 0.436627 5_4:+27761238 Msa0846920:CDS
GCCCATTATTGTCATGGTTG+AGG 0.483681 5_4:+27761081 Msa0846920:CDS
GTATGGTGGCTGCTTGCCTT+CGG 0.489568 5_4:+27761222 Msa0846920:CDS
AAGCAAGCATCAAATTTATG+AGG 0.496655 5_4:-27761455 None:intergenic
ATTCAAATGTTAGAAATGGA+TGG 0.501542 5_4:+27761485 Msa0846920:CDS
GAGAAGGCTTTGTAGAAAAG+AGG 0.518294 5_4:-27761294 None:intergenic
GTTCGTGCCCATTATTGTCA+TGG 0.547245 5_4:+27761075 Msa0846920:CDS
TTCAAATGTTAGAAATGGAT+GGG 0.548625 5_4:+27761486 Msa0846920:CDS
TAACAGCACTGAATTTGATA+GGG 0.556919 5_4:+27761196 Msa0846920:CDS
CCATAGCAAGAAAATATTGG+TGG 0.586834 5_4:+27761316 Msa0846920:CDS
TTCGGCAAGTTCTTGGAATG+GGG 0.612266 5_4:+27761240 Msa0846920:CDS
TGACAATTGCATAAACCAGA+AGG 0.629214 5_4:+27761343 Msa0846920:CDS
TTGTCATGGTTGAGGACACA+TGG 0.655815 5_4:+27761089 Msa0846920:CDS
ATTTGATAGGGCTAGTATGG+TGG 0.659564 5_4:+27761208 Msa0846920:CDS
CCATTCCAAGAACTTGCCGA+AGG 0.677575 5_4:-27761238 None:intergenic
GATGTGAAGTGTGTTGAAAG+CGG 0.691717 5_4:+27761404 Msa0846920:CDS
TCATGGTTGAGGACACATGG+TGG 0.761656 5_4:+27761092 Msa0846920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAGATATATAAACTTATAA+AGG - chr5_4:27760458-27760477 None:intergenic 15.0%
!! ACAGATATATAAACTTATAA+AGG - chr5_4:27760458-27760477 None:intergenic 15.0%
!!! CAAAATTTAGTTCACAAATT+TGG + chr5_4:27761007-27761026 Msa0846920:intron 20.0%
!!! CAAAATTTAGTTCACAAATT+TGG + chr5_4:27761007-27761026 Msa0846920:intron 20.0%
! ATATATCTGTGTGTTATCAT+TGG + chr5_4:27760468-27760487 Msa0846920:intron 25.0%
! AAGTTGAGATGTTAATTGAT+TGG + chr5_4:27760569-27760588 Msa0846920:intron 25.0%
! AGCAATGAAACTAAGAAAAT+TGG - chr5_4:27760604-27760623 None:intergenic 25.0%
!! TTTTCTTAGTTTCATTGCTT+TGG + chr5_4:27760605-27760624 Msa0846920:intron 25.0%
! GATTAAAGCCATATTTGATA+AGG + chr5_4:27760630-27760649 Msa0846920:intron 25.0%
!! TTTGAGATTGGAAAATCAAT+AGG + chr5_4:27760939-27760958 Msa0846920:intron 25.0%
! AATCAATATACTTGTCTATG+TGG + chr5_4:27761046-27761065 Msa0846920:intron 25.0%
!! AAATTCAGTGCTGTTAATAT+TGG - chr5_4:27761192-27761211 None:intergenic 25.0%
! ATATATCTGTGTGTTATCAT+TGG + chr5_4:27760468-27760487 Msa0846920:intron 25.0%
! AAGTTGAGATGTTAATTGAT+TGG + chr5_4:27760569-27760588 Msa0846920:intron 25.0%
! AGCAATGAAACTAAGAAAAT+TGG - chr5_4:27760604-27760623 None:intergenic 25.0%
!! TTTTCTTAGTTTCATTGCTT+TGG + chr5_4:27760605-27760624 Msa0846920:intron 25.0%
! GATTAAAGCCATATTTGATA+AGG + chr5_4:27760630-27760649 Msa0846920:intron 25.0%
!! TTTGAGATTGGAAAATCAAT+AGG + chr5_4:27760939-27760958 Msa0846920:intron 25.0%
! AATCAATATACTTGTCTATG+TGG + chr5_4:27761046-27761065 Msa0846920:intron 25.0%
!! AAATTCAGTGCTGTTAATAT+TGG - chr5_4:27761192-27761211 None:intergenic 25.0%
! AACTAGCTTCATGTAAAACT+GGG - chr5_4:27760704-27760723 None:intergenic 30.0%
!! TTTTACATGAAGCTAGTTCT+AGG + chr5_4:27760706-27760725 Msa0846920:intron 30.0%
CTCAAGTAGTTATTACATGA+CGG - chr5_4:27760879-27760898 None:intergenic 30.0%
!!! TCAGTTCTTTTCTTTGAGAT+TGG + chr5_4:27760927-27760946 Msa0846920:intron 30.0%
! GTTGCTAGAAGAATCATTTT+GGG - chr5_4:27761170-27761189 None:intergenic 30.0%
! TTAACAGCACTGAATTTGAT+AGG + chr5_4:27761195-27761214 Msa0846920:CDS 30.0%
! TAACAGCACTGAATTTGATA+GGG + chr5_4:27761196-27761215 Msa0846920:CDS 30.0%
!! ATATTTTCTTGCTATGGAGA+AGG - chr5_4:27761313-27761332 None:intergenic 30.0%
TCTCCATAGCAAGAAAATAT+TGG + chr5_4:27761313-27761332 Msa0846920:CDS 30.0%
GCAGATGCATTAAAAAAGTT+TGG + chr5_4:27761377-27761396 Msa0846920:CDS 30.0%
AAGCAAGCATCAAATTTATG+AGG - chr5_4:27761458-27761477 None:intergenic 30.0%
TAAATTTGATGCTTGCTTCA+TGG + chr5_4:27761460-27761479 Msa0846920:CDS 30.0%
GGACATTCAAATGTTAGAAA+TGG + chr5_4:27761481-27761500 Msa0846920:CDS 30.0%
! AACTAGCTTCATGTAAAACT+GGG - chr5_4:27760704-27760723 None:intergenic 30.0%
!! TTTTACATGAAGCTAGTTCT+AGG + chr5_4:27760706-27760725 Msa0846920:intron 30.0%
CTCAAGTAGTTATTACATGA+CGG - chr5_4:27760879-27760898 None:intergenic 30.0%
!!! TCAGTTCTTTTCTTTGAGAT+TGG + chr5_4:27760927-27760946 Msa0846920:intron 30.0%
! GTTGCTAGAAGAATCATTTT+GGG - chr5_4:27761170-27761189 None:intergenic 30.0%
! TTAACAGCACTGAATTTGAT+AGG + chr5_4:27761195-27761214 Msa0846920:CDS 30.0%
! TAACAGCACTGAATTTGATA+GGG + chr5_4:27761196-27761215 Msa0846920:CDS 30.0%
!! ATATTTTCTTGCTATGGAGA+AGG - chr5_4:27761313-27761332 None:intergenic 30.0%
TCTCCATAGCAAGAAAATAT+TGG + chr5_4:27761313-27761332 Msa0846920:CDS 30.0%
GCAGATGCATTAAAAAAGTT+TGG + chr5_4:27761377-27761396 Msa0846920:CDS 30.0%
AAGCAAGCATCAAATTTATG+AGG - chr5_4:27761458-27761477 None:intergenic 30.0%
TAAATTTGATGCTTGCTTCA+TGG + chr5_4:27761460-27761479 Msa0846920:CDS 30.0%
GGACATTCAAATGTTAGAAA+TGG + chr5_4:27761481-27761500 Msa0846920:CDS 30.0%
!!! CAGCATTTTCTAAAGCTTAG+TGG + chr5_4:27760384-27760403 Msa0846920:intron 35.0%
!! TTTCTAAAGCTTAGTGGTAG+TGG + chr5_4:27760390-27760409 Msa0846920:intron 35.0%
! GGAGAAATCTGACTTGAAAA+AGG + chr5_4:27760411-27760430 Msa0846920:intron 35.0%
CATCAAATCTATCTGATTGC+TGG + chr5_4:27760532-27760551 Msa0846920:intron 35.0%
!! CTCTGGAACCTTATCAAATA+TGG - chr5_4:27760641-27760660 None:intergenic 35.0%
AAAGTAAAATTGCACTGCTC+TGG - chr5_4:27760658-27760677 None:intergenic 35.0%
! AGAGCAGTGCAATTTTACTT+TGG + chr5_4:27760657-27760676 Msa0846920:intron 35.0%
! GAACTAGCTTCATGTAAAAC+TGG - chr5_4:27760705-27760724 None:intergenic 35.0%
GTCAAACATTGCACTTGAAT+TGG + chr5_4:27760738-27760757 Msa0846920:intron 35.0%
TCCTCAACCATGACAATAAT+GGG - chr5_4:27761085-27761104 None:intergenic 35.0%
! GGTTGCTAGAAGAATCATTT+TGG - chr5_4:27761171-27761190 None:intergenic 35.0%
! TGAATTTGATAGGGCTAGTA+TGG + chr5_4:27761205-27761224 Msa0846920:CDS 35.0%
TTCTTGGAATGGGGTAAAAA+AGG + chr5_4:27761249-27761268 Msa0846920:intron 35.0%
ATGGGGTAAAAAAGGAAGAT+AGG + chr5_4:27761257-27761276 Msa0846920:intron 35.0%
TGGGGTAAAAAAGGAAGATA+GGG + chr5_4:27761258-27761277 Msa0846920:intron 35.0%
! CCACCAATATTTTCTTGCTA+TGG - chr5_4:27761319-27761338 None:intergenic 35.0%
CCATAGCAAGAAAATATTGG+TGG + chr5_4:27761316-27761335 Msa0846920:CDS 35.0%
TGACAATTGCATAAACCAGA+AGG + chr5_4:27761343-27761362 Msa0846920:CDS 35.0%
!!! CAGCATTTTCTAAAGCTTAG+TGG + chr5_4:27760384-27760403 Msa0846920:intron 35.0%
!! TTTCTAAAGCTTAGTGGTAG+TGG + chr5_4:27760390-27760409 Msa0846920:intron 35.0%
! GGAGAAATCTGACTTGAAAA+AGG + chr5_4:27760411-27760430 Msa0846920:intron 35.0%
CATCAAATCTATCTGATTGC+TGG + chr5_4:27760532-27760551 Msa0846920:intron 35.0%
!! CTCTGGAACCTTATCAAATA+TGG - chr5_4:27760641-27760660 None:intergenic 35.0%
AAAGTAAAATTGCACTGCTC+TGG - chr5_4:27760658-27760677 None:intergenic 35.0%
! AGAGCAGTGCAATTTTACTT+TGG + chr5_4:27760657-27760676 Msa0846920:intron 35.0%
! GAACTAGCTTCATGTAAAAC+TGG - chr5_4:27760705-27760724 None:intergenic 35.0%
GTCAAACATTGCACTTGAAT+TGG + chr5_4:27760738-27760757 Msa0846920:intron 35.0%
TCCTCAACCATGACAATAAT+GGG - chr5_4:27761085-27761104 None:intergenic 35.0%
! GGTTGCTAGAAGAATCATTT+TGG - chr5_4:27761171-27761190 None:intergenic 35.0%
! TGAATTTGATAGGGCTAGTA+TGG + chr5_4:27761205-27761224 Msa0846920:CDS 35.0%
TTCTTGGAATGGGGTAAAAA+AGG + chr5_4:27761249-27761268 Msa0846920:intron 35.0%
ATGGGGTAAAAAAGGAAGAT+AGG + chr5_4:27761257-27761276 Msa0846920:intron 35.0%
TGGGGTAAAAAAGGAAGATA+GGG + chr5_4:27761258-27761277 Msa0846920:intron 35.0%
! CCACCAATATTTTCTTGCTA+TGG - chr5_4:27761319-27761338 None:intergenic 35.0%
CCATAGCAAGAAAATATTGG+TGG + chr5_4:27761316-27761335 Msa0846920:CDS 35.0%
TGACAATTGCATAAACCAGA+AGG + chr5_4:27761343-27761362 Msa0846920:CDS 35.0%
TGAATTGGCATGTAGCAAAG+AGG + chr5_4:27760753-27760772 Msa0846920:intron 40.0%
GAATTGGCATGTAGCAAAGA+GGG + chr5_4:27760754-27760773 Msa0846920:intron 40.0%
TGTCATTGCTCACTGAATCA+TGG + chr5_4:27760793-27760812 Msa0846920:intron 40.0%
GTCCTCAACCATGACAATAA+TGG - chr5_4:27761086-27761105 None:intergenic 40.0%
! ATTTGATAGGGCTAGTATGG+TGG + chr5_4:27761208-27761227 Msa0846920:CDS 40.0%
! GAGAAGGCTTTGTAGAAAAG+AGG - chr5_4:27761297-27761316 None:intergenic 40.0%
GATGTGAAGTGTGTTGAAAG+CGG + chr5_4:27761404-27761423 Msa0846920:CDS 40.0%
TGAATTGGCATGTAGCAAAG+AGG + chr5_4:27760753-27760772 Msa0846920:intron 40.0%
GAATTGGCATGTAGCAAAGA+GGG + chr5_4:27760754-27760773 Msa0846920:intron 40.0%
TGTCATTGCTCACTGAATCA+TGG + chr5_4:27760793-27760812 Msa0846920:intron 40.0%
GTCCTCAACCATGACAATAA+TGG - chr5_4:27761086-27761105 None:intergenic 40.0%
! ATTTGATAGGGCTAGTATGG+TGG + chr5_4:27761208-27761227 Msa0846920:CDS 40.0%
! GAGAAGGCTTTGTAGAAAAG+AGG - chr5_4:27761297-27761316 None:intergenic 40.0%
GATGTGAAGTGTGTTGAAAG+CGG + chr5_4:27761404-27761423 Msa0846920:CDS 40.0%
ACTGAATCATGGTACATGCG+AGG + chr5_4:27760804-27760823 Msa0846920:intron 45.0%
GCTAAATCCATTCCACACCA+AGG + chr5_4:27760846-27760865 Msa0846920:intron 45.0%
ATCCATTCCACACCAAGGAT+TGG + chr5_4:27760851-27760870 Msa0846920:intron 45.0%
GTTCGTGCCCATTATTGTCA+TGG + chr5_4:27761075-27761094 Msa0846920:CDS 45.0%
GCCCATTATTGTCATGGTTG+AGG + chr5_4:27761081-27761100 Msa0846920:CDS 45.0%
TTGTCATGGTTGAGGACACA+TGG + chr5_4:27761089-27761108 Msa0846920:CDS 45.0%
CTTCGGCAAGTTCTTGGAAT+GGG + chr5_4:27761239-27761258 Msa0846920:CDS 45.0%
TTCGGCAAGTTCTTGGAATG+GGG + chr5_4:27761240-27761259 Msa0846920:CDS 45.0%
ACTGAATCATGGTACATGCG+AGG + chr5_4:27760804-27760823 Msa0846920:intron 45.0%
GCTAAATCCATTCCACACCA+AGG + chr5_4:27760846-27760865 Msa0846920:intron 45.0%
ATCCATTCCACACCAAGGAT+TGG + chr5_4:27760851-27760870 Msa0846920:intron 45.0%
GTTCGTGCCCATTATTGTCA+TGG + chr5_4:27761075-27761094 Msa0846920:CDS 45.0%
GCCCATTATTGTCATGGTTG+AGG + chr5_4:27761081-27761100 Msa0846920:CDS 45.0%
TTGTCATGGTTGAGGACACA+TGG + chr5_4:27761089-27761108 Msa0846920:CDS 45.0%
CTTCGGCAAGTTCTTGGAAT+GGG + chr5_4:27761239-27761258 Msa0846920:CDS 45.0%
TTCGGCAAGTTCTTGGAATG+GGG + chr5_4:27761240-27761259 Msa0846920:CDS 45.0%
! TGCCAATCCTTGGTGTGGAA+TGG - chr5_4:27760856-27760875 None:intergenic 50.0%
! TACATGACGGTGCCAATCCT+TGG - chr5_4:27760866-27760885 None:intergenic 50.0%
TCATGGTTGAGGACACATGG+TGG + chr5_4:27761092-27761111 Msa0846920:CDS 50.0%
CCATTCCAAGAACTTGCCGA+AGG - chr5_4:27761241-27761260 None:intergenic 50.0%
CCTTCGGCAAGTTCTTGGAA+TGG + chr5_4:27761238-27761257 Msa0846920:CDS 50.0%
! TGCCAATCCTTGGTGTGGAA+TGG - chr5_4:27760856-27760875 None:intergenic 50.0%
! TACATGACGGTGCCAATCCT+TGG - chr5_4:27760866-27760885 None:intergenic 50.0%
TCATGGTTGAGGACACATGG+TGG + chr5_4:27761092-27761111 Msa0846920:CDS 50.0%
CCATTCCAAGAACTTGCCGA+AGG - chr5_4:27761241-27761260 None:intergenic 50.0%
CCTTCGGCAAGTTCTTGGAA+TGG + chr5_4:27761238-27761257 Msa0846920:CDS 50.0%
GTATGGTGGCTGCTTGCCTT+CGG + chr5_4:27761222-27761241 Msa0846920:CDS 55.0%
GCTTGCCTTCGGCAAGTTCT+TGG + chr5_4:27761233-27761252 Msa0846920:CDS 55.0%
GTATGGTGGCTGCTTGCCTT+CGG + chr5_4:27761222-27761241 Msa0846920:CDS 55.0%
GCTTGCCTTCGGCAAGTTCT+TGG + chr5_4:27761233-27761252 Msa0846920:CDS 55.0%
! GACGGTGCCAATCCTTGGTG+TGG - chr5_4:27760861-27760880 None:intergenic 60.0%
CTGCAGCTGCAGCTACCTTC+TGG - chr5_4:27761361-27761380 None:intergenic 60.0%
! GACGGTGCCAATCCTTGGTG+TGG - chr5_4:27760861-27760880 None:intergenic 60.0%
CTGCAGCTGCAGCTACCTTC+TGG - chr5_4:27761361-27761380 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 27760375 27761505 27760375 ID=Msa0846920;Name=Msa0846920
chr5_4 mRNA 27760375 27761505 27760375 ID=Msa0846920-mRNA-1;Parent=Msa0846920;Name=Msa0846920-mRNA-1;_AED=0.42;_eAED=0.43;_QI=0|0|0|1|0.33|0|4|0|146
chr5_4 exon 27760375 27760381 27760375 ID=Msa0846920-mRNA-1:exon:10634;Parent=Msa0846920-mRNA-1
chr5_4 exon 27760971 27760976 27760971 ID=Msa0846920-mRNA-1:exon:10635;Parent=Msa0846920-mRNA-1
chr5_4 exon 27761072 27761261 27761072 ID=Msa0846920-mRNA-1:exon:10636;Parent=Msa0846920-mRNA-1
chr5_4 exon 27761271 27761505 27761271 ID=Msa0846920-mRNA-1:exon:10637;Parent=Msa0846920-mRNA-1
chr5_4 CDS 27760375 27760381 27760375 ID=Msa0846920-mRNA-1:cds;Parent=Msa0846920-mRNA-1
chr5_4 CDS 27760971 27760976 27760971 ID=Msa0846920-mRNA-1:cds;Parent=Msa0846920-mRNA-1
chr5_4 CDS 27761072 27761261 27761072 ID=Msa0846920-mRNA-1:cds;Parent=Msa0846920-mRNA-1
chr5_4 CDS 27761271 27761505 27761271 ID=Msa0846920-mRNA-1:cds;Parent=Msa0846920-mRNA-1
Gene Sequence

>Msa0846920

ATGAAATTTCAGAAATGTTCGTGCCCATTATTGTCATGGTTGAGGACACATGGTGGTTTCAATGTATGTAAACAAGACTTGAAACAGAATGAAAATATATTTAAAATTCCCAAAATGATTCTTCTAGCAACCAATATTAACAGCACTGAATTTGATAGGGCTAGTATGGTGGCTGCTTGCCTTCGGCAAGTTCTTGGAATGGGGAAGATAGGGAAAGAAAATAGCTCCTCTTTTCTACAAAGCCTTCTCCATAGCAAGAAAATATTGGTGGTCTATGACAATTGCATAAACCAGAAGGTAGCTGCAGCTGCAGATGCATTAAAAAAGTTTGGAGCTGATGTGAAGTGTGTTGAAAGCGGCAAAGCTGCACTTGAAATGCTTCAATTGCCTCATAAATTTGATGCTTGCTTCATGGACATTCAAATGTTAGAAATGGAT

Protein sequence

>Msa0846920

MKFQKCSCPLLSWLRTHGGFNVCKQDLKQNENIFKIPKMILLATNINSTEFDRASMVAACLRQVLGMGKIGKENSSSFLQSLLHSKKILVVYDNCINQKVAAAADALKKFGADVKCVESGKAALEMLQLPHKFDACFMDIQMLEMD