AlfalfaGEDB | Msa0847000

M. sativa cultivar zhongmu-4 / Msa0847000

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847000	AES97051.1	90.909	330	30	0	1	330	1	330	0.0	624

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847000	sp\|Q8L3W1\|VRN1_ARATH	28.571	343	184	9	16	306	6	339	1.50e-33	129

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847000	G7JXZ7	90.909	330	30	0	1	330	1	330	0.0	624

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TFs/TRs:

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Gene ID	Type	Classification
Msa0847000	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847000	MtrunA17_Chr5g0419201	88.136	177	21	0	1	177	1	177	7.25e-114	328
Msa0847000	MtrunA17_Chr5g0419211	90.217	92	9	0	223	314	10	101	1.65e-53	171
Msa0847000	MtrunA17_Chr3g0131891	33.007	306	180	9	16	310	19	310	2.86e-45	157
Msa0847000	MtrunA17_Chr7g0233561	32.039	309	181	10	16	306	18	315	5.90e-43	150
Msa0847000	MtrunA17_Chr8g0390841	28.358	335	188	8	2	309	28	337	2.98e-42	153
Msa0847000	MtrunA17_Chr1g0208771	29.967	307	165	8	32	310	13	297	3.89e-40	146
Msa0847000	MtrunA17_Chr7g0233541	30.619	307	184	7	31	318	1	297	6.92e-40	142
Msa0847000	MtrunA17_Chr1g0163261	30.938	320	184	9	10	317	3	297	7.83e-39	139
Msa0847000	MtrunA17_Chr1g0163181	31.034	290	187	5	25	307	14	297	1.60e-38	141
Msa0847000	MtrunA17_Chr7g0233531	33.216	283	165	7	31	306	1	266	2.83e-38	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0847000	AT3G18990.2	28.814	354	186	10	16	317	6	345	9.21e-36	133
Msa0847000	AT3G18990.1	28.571	343	184	9	16	306	6	339	1.52e-34	129
Msa0847000	AT4G01580.1	38.095	147	75	4	16	161	30	161	9.96e-20	85.9
Msa0847000	AT3G18960.2	41.414	99	58	0	16	114	30	128	1.04e-17	80.5
Msa0847000	AT3G18960.1	41.414	99	58	0	16	114	30	128	1.22e-17	80.5
Msa0847000	AT4G33280.1	22.945	292	204	7	30	306	37	322	1.20e-16	80.1
Msa0847000	AT1G49475.1	32.692	156	94	3	16	166	34	183	4.45e-16	75.9
Msa0847000	AT3G18960.3	42.169	83	48	0	32	114	9	91	1.22e-13	68.6
Msa0847000	AT5G09780.1	23.529	306	203	11	11	299	14	305	8.02e-13	68.6

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Find 54 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATGAAAACCAGTGA+TGG	0.566067	5_4:-28171717	Msa0847000:intron
AAACACTACAAAATACTAAA+AGG	0.340468	5_4:-28171448	Msa0847000:CDS
AAAGGATGGGATTGCAACCG+TGG	0.682554	5_4:-28171194	Msa0847000:CDS
AACACTATTCAATGAGTTAT+GGG	0.394195	5_4:-28171962	Msa0847000:CDS
AACCGTGGTGTTTGGTGATG+AGG	0.573245	5_4:-28171179	Msa0847000:CDS
AATATTTGAATGGAAAGGAT+GGG	0.537959	5_4:-28171207	Msa0847000:CDS
AATCGAGCACTCATAACTTC+TGG	0.465532	5_4:-28171115	Msa0847000:CDS
ACCATATGTTCAAGGTAGGA+TGG	0.542632	5_4:+28171374	None:intergenic
ACGTTGTGTTTCAAAATGGT+TGG	0.559115	5_4:-28171998	Msa0847000:CDS
ACTTCCCATTTATCACCATT+TGG	0.427050	5_4:+28172044	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATTTCTTCACAAAACTCCT+AGG	+	chr5_4:28171054-28171073	None:intergenic	30.0%
TTCTACCAAATGGTGATAAA+TGG	-	chr5_4:28171106-28171125	Msa0847000:CDS	30.0%
TCTACCAAATGGTGATAAAT+GGG	-	chr5_4:28171107-28171126	Msa0847000:CDS	30.0%
GTGATAAATGGGAAGTAAAT+TGG	-	chr5_4:28171118-28171137	Msa0847000:CDS	30.0%
CAACACTATTCAATGAGTTA+TGG	-	chr5_4:28171192-28171211	Msa0847000:CDS	30.0%
TTCTTTCAACTTGATCTCTA+GGG	+	chr5_4:28171314-28171333	None:intergenic	30.0%
TTTCTTTCAACTTGATCTCT+AGG	+	chr5_4:28171315-28171334	None:intergenic	30.0%
AAAAGAATGAAAACCAGTGA+TGG	-	chr5_4:28171438-28171457	Msa0847000:CDS	30.0%
TCAAAGGAAAAACCTAAAGA+AGG	-	chr5_4:28171467-28171486	Msa0847000:CDS	30.0%
TTTACAATTTCAGGAGGTAA+TGG	-	chr5_4:28171628-28171647	Msa0847000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	28170806	28172371	28170806	ID=Msa0847000;Name=Msa0847000
chr5_4	mRNA	28170806	28172371	28170806	ID=Msa0847000-mRNA-1;Parent=Msa0847000;Name=Msa0847000-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|5\|0\|330
chr5_4	exon	28172282	28172371	28172282	ID=Msa0847000-mRNA-1:exon:10694;Parent=Msa0847000-mRNA-1
chr5_4	exon	28171718	28172132	28171718	ID=Msa0847000-mRNA-1:exon:10693;Parent=Msa0847000-mRNA-1
chr5_4	exon	28171376	28171536	28171376	ID=Msa0847000-mRNA-1:exon:10692;Parent=Msa0847000-mRNA-1
chr5_4	exon	28170978	28171254	28170978	ID=Msa0847000-mRNA-1:exon:10691;Parent=Msa0847000-mRNA-1
chr5_4	exon	28170806	28170855	28170806	ID=Msa0847000-mRNA-1:exon:10690;Parent=Msa0847000-mRNA-1
chr5_4	CDS	28172282	28172371	28172282	ID=Msa0847000-mRNA-1:cds;Parent=Msa0847000-mRNA-1
chr5_4	CDS	28171718	28172132	28171718	ID=Msa0847000-mRNA-1:cds;Parent=Msa0847000-mRNA-1
chr5_4	CDS	28171376	28171536	28171376	ID=Msa0847000-mRNA-1:cds;Parent=Msa0847000-mRNA-1
chr5_4	CDS	28170978	28171254	28170978	ID=Msa0847000-mRNA-1:cds;Parent=Msa0847000-mRNA-1
chr5_4	CDS	28170806	28170855	28170806	ID=Msa0847000-mRNA-1:cds;Parent=Msa0847000-mRNA-1

Gene Sequence

>Msa0847000

ATGTCTTCTCCGAGTGTTGTTCATCAGTTCCATAAGTCACAATGTTTTTTCAAAGTCATCGTCGAGAAAACCCTTAAAAAGAAAGAGATTATGATTCCTAGGAGTTTTGTGAAGAAATATGGGAAAAAATTATCAAACCCAGTAACCCTAGTTCTACCAAATGGTGATAAATGGGAAGTAAATTGGATAAAACGTGACCATGACGTTGTGTTTCAAAATGGTTGGGAAAAATTTGCACAACACTATTCAATGAGTTATGGGCATTTTTTAGTTTTTAGACTTGAAACAAGGTCTCAATTTCAAGTAATGATATTTGACAAAAGCGCTTTGGAAATGGATTATTGGAGTATACCAAGCCCTAGAGATCAAGTTGAAAGAAACATTCATCATCATGAAGATGCTGAAAAGAGTGACGATAAAGACGATTATATTGAGATTTCGGATGAGAGTGAAATGCGAAGATCTTCATCACCTCGACCTCAGAAAAGAATGAAAACCAGTGATGGAGGTAATGGAGAAAATTTCTTCAAAGCAAAGAATGACTCATCAAATGAAGCTATGAATTGGAAAGAAACACTACAAAATACTAAAAGGAGAGCCATGAATTTTCATTCCGATAACCCCTTTTTCATTCGCCCGTTGCATCCATCCTACCTTGAACATATGTTTATACCAACATCTTTTTCCAAAGAATATTTGAATGGAAAGGATGGGATTGCAACCGTGGTGTTTGGTGATGAGGATAAGACTTGGTCCTTAAACTTCAAGTTCAATGCTACCACTAATCGAGCACTCATAACTTCTGGTTGGAGACAAATTGTTGAGGAATATAATTTCAAAGTTGGTGACACTTGTGTGTTTGAGATGACTGATAGTACAAATATCTTATTTAAAGTCTATATTGTTCGAGCCAATGATCAATCTAGCTCCGAACTTTTTCAAGATTCTGGAAATCCTAAAATGAAGAGGAAATCAAATTCAAGACAAAACTGA

Protein sequence

>Msa0847000

MSSPSVVHQFHKSQCFFKVIVEKTLKKKEIMIPRSFVKKYGKKLSNPVTLVLPNGDKWEVNWIKRDHDVVFQNGWEKFAQHYSMSYGHFLVFRLETRSQFQVMIFDKSALEMDYWSIPSPRDQVERNIHHHEDAEKSDDKDDYIEISDESEMRRSSSPRPQKRMKTSDGGNGENFFKAKNDSSNEAMNWKETLQNTKRRAMNFHSDNPFFIRPLHPSYLEHMFIPTSFSKEYLNGKDGIATVVFGDEDKTWSLNFKFNATTNRALITSGWRQIVEEYNFKVGDTCVFEMTDSTNILFKVYIVRANDQSSSELFQDSGNPKMKRKSNSRQN*