AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0848740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0848740 MtrunA17_Chr5g0422571 92.834 307 10 3 1 307 1 295 0.0 586
Msa0848740 MtrunA17_Chr1g0198651 70.120 251 60 5 21 269 23 260 9.41e-116 335
Msa0848740 MtrunA17_Chr2g0328921 67.890 218 56 4 68 274 84 298 1.21e-106 313
Msa0848740 MtrunA17_Chr1g0173841 61.538 247 60 4 71 288 144 384 5.26e-106 314
Msa0848740 MtrunA17_Chr7g0258011 62.821 234 60 3 76 288 118 345 1.31e-103 306
Msa0848740 MtrunA17_Chr8g0352011 62.174 230 64 5 76 288 112 335 1.22e-102 304
Msa0848740 MtrunA17_Chr2g0321291 62.009 229 68 5 63 274 68 294 3.64e-100 296
Msa0848740 MtrunA17_Chr4g0026521 63.470 219 64 4 78 283 76 291 2.18e-98 291
Msa0848740 MtrunA17_Chr4g0053341 64.390 205 63 3 68 269 32 229 1.43e-94 279
Msa0848740 MtrunA17_Chr1g0200421 38.596 171 87 8 108 264 226 392 1.19e-24 103
Msa0848740 MtrunA17_Chr1g0181381 33.163 196 113 5 77 263 10 196 1.72e-23 100
Msa0848740 MtrunA17_Chr8g0382121 40.278 144 80 5 128 267 127 268 2.14e-23 97.8
Msa0848740 MtrunA17_Chr4g0029521 33.000 200 123 6 64 261 4 194 4.89e-23 99.0
Msa0848740 MtrunA17_Chr4g0040571 31.771 192 120 4 85 275 27 208 2.10e-22 97.1
Msa0848740 MtrunA17_Chr2g0320341 38.065 155 88 5 116 264 235 387 3.65e-22 95.5
Msa0848740 MtrunA17_Chr1g0151351 35.057 174 101 6 89 260 33 196 6.47e-22 95.5
Msa0848740 MtrunA17_Chr1g0199101 36.306 157 87 6 113 267 63 208 1.03e-21 95.1
Msa0848740 MtrunA17_Chr7g0263941 33.889 180 106 6 86 263 23 191 1.73e-21 94.0
Msa0848740 MtrunA17_Chr8g0373541 33.005 203 112 7 93 281 26 218 2.11e-21 94.4
Msa0848740 MtrunA17_Chr1g0185121 30.841 214 135 6 66 276 8 211 2.15e-21 94.0
Msa0848740 MtrunA17_Chr8g0342421 34.868 152 86 5 120 269 85 225 2.44e-21 94.0
Msa0848740 MtrunA17_Chr2g0330171 35.443 158 88 6 108 263 58 203 4.14e-21 93.2
Msa0848740 MtrunA17_Chr7g0268961 37.500 144 77 6 120 261 45 177 4.31e-21 93.2
Msa0848740 MtrunA17_Chr2g0326381 38.571 140 76 4 120 258 55 185 9.99e-21 91.7
Msa0848740 MtrunA17_Chr1g0210821 35.374 147 84 4 118 263 65 201 1.25e-20 92.0
Msa0848740 MtrunA17_Chr1g0191991 35.443 158 83 6 114 263 74 220 2.37e-20 91.3
Msa0848740 MtrunA17_Chr1g0162971 37.671 146 78 5 120 263 64 198 2.56e-20 90.9
Msa0848740 MtrunA17_Chr1g0163001 35.294 153 86 5 113 263 59 200 7.66e-20 89.7
Msa0848740 MtrunA17_Chr3g0141431 33.721 172 102 6 92 261 17 178 3.32e-19 87.4
Msa0848740 MtrunA17_Chr3g0123941 39.583 144 78 7 128 267 139 277 3.84e-19 86.3
Msa0848740 MtrunA17_Chr2g0324991 36.806 144 78 7 113 253 8 141 1.87e-17 77.8
Msa0848740 MtrunA17_Chr6g0479921 30.612 147 89 6 118 261 50 186 4.11e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0848740 AT1G13290.1 67.681 263 57 6 21 278 12 251 1.85e-117 340
Msa0848740 AT3G57670.1 72.632 190 40 2 108 297 199 376 7.03e-101 301
Msa0848740 AT1G51220.1 50.637 314 116 7 7 292 34 336 3.35e-100 298
Msa0848740 AT3G20880.1 63.594 217 46 4 54 264 204 393 2.07e-97 293
Msa0848740 AT1G08290.1 70.056 177 45 1 110 286 164 332 1.36e-95 286
Msa0848740 AT1G34790.1 63.333 210 64 3 76 273 83 291 2.03e-91 274
Msa0848740 AT1G34370.3 38.235 170 91 6 108 264 9 177 6.54e-27 107
Msa0848740 AT1G34370.2 40.397 151 83 5 120 264 238 387 2.18e-26 108
Msa0848740 AT1G34370.1 40.397 151 83 5 120 264 238 387 2.18e-26 108
Msa0848740 AT5G22890.1 39.785 186 94 11 91 264 181 360 1.69e-24 102
Msa0848740 AT5G66730.1 32.673 202 121 6 71 268 4 194 1.04e-22 98.2
Msa0848740 AT1G55110.3 37.013 154 85 5 118 269 84 227 5.09e-22 96.3
Msa0848740 AT1G55110.1 37.013 154 85 5 118 269 84 227 5.09e-22 96.3
Msa0848740 AT1G55110.2 37.013 154 85 5 118 269 84 227 5.09e-22 96.3
Msa0848740 AT3G50700.1 32.673 202 123 5 71 269 6 197 9.65e-22 95.1
Msa0848740 AT5G03150.1 37.063 143 80 4 120 261 76 209 1.27e-21 95.1
Msa0848740 AT5G44160.1 33.523 176 106 4 94 268 34 199 1.62e-21 94.7
Msa0848740 AT2G02080.1 37.255 153 83 5 118 268 75 216 1.83e-21 94.7
Msa0848740 AT2G02080.4 37.255 153 83 5 118 268 75 216 1.83e-21 94.7
Msa0848740 AT2G02080.5 37.255 153 83 5 118 268 75 216 1.83e-21 94.7
Msa0848740 AT2G02080.3 37.255 153 83 5 118 268 75 216 1.83e-21 94.7
Msa0848740 AT2G02070.2 37.255 153 83 5 118 268 73 214 2.09e-21 94.7
Msa0848740 AT2G02070.1 37.255 153 83 5 118 268 73 214 2.09e-21 94.7
Msa0848740 AT1G03840.1 33.523 176 106 5 94 268 38 203 2.62e-21 94.4
Msa0848740 AT1G03840.2 34.091 176 103 7 94 268 38 201 4.52e-21 93.6
Msa0848740 AT5G44160.2 36.000 150 85 4 120 268 21 160 5.14e-21 93.2
Msa0848740 AT2G02080.6 37.086 151 82 5 121 269 1 140 7.67e-21 92.4
Msa0848740 AT2G02080.2 37.086 151 82 5 121 269 1 140 7.67e-21 92.4
Msa0848740 AT1G14580.1 36.986 146 79 5 118 261 74 208 4.26e-20 90.5
Msa0848740 AT1G14580.2 36.986 146 79 5 118 261 74 208 4.26e-20 90.5
Msa0848740 AT1G14580.3 36.986 146 79 5 118 261 88 222 4.57e-20 90.5
Msa0848740 AT3G45260.2 35.333 150 87 4 120 268 62 202 5.79e-20 90.1
Msa0848740 AT3G45260.1 35.333 150 87 4 120 268 62 202 5.79e-20 90.1
Msa0848740 AT5G60470.2 34.104 173 104 5 92 263 39 202 6.14e-20 90.1
Msa0848740 AT5G60470.3 34.104 173 104 5 92 263 35 198 6.90e-20 90.1
Msa0848740 AT3G13810.2 34.615 156 92 4 113 267 87 233 8.39e-20 90.1
Msa0848740 AT3G13810.4 34.615 156 92 4 113 267 86 232 9.37e-20 89.7
Msa0848740 AT3G13810.1 34.615 156 92 4 113 267 86 232 9.37e-20 89.7
Msa0848740 AT5G60470.1 37.241 145 81 5 120 263 58 193 9.69e-20 89.4
Msa0848740 AT3G13810.5 34.615 156 92 4 113 267 81 227 9.98e-20 89.7
Msa0848740 AT3G13810.3 34.615 156 92 4 113 267 73 219 1.05e-19 89.7
Msa0848740 AT3G13810.6 35.099 151 88 4 118 267 72 213 1.08e-19 89.7
Msa0848740 AT4G02670.2 34.211 152 90 4 113 263 71 213 8.61e-19 86.3
Msa0848740 AT4G02670.1 34.211 152 90 4 113 263 69 211 9.45e-19 86.3
Msa0848740 AT1G25250.1 30.216 139 88 4 118 254 31 162 1.89e-11 64.7
Msa0848740 AT1G25250.3 30.216 139 88 4 118 254 54 185 1.93e-11 64.7

Find 66 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACTCCCCTTAGAGATTC+TGG 0.179929 5_4:-34483747 None:intergenic
CTCCTATTCAAGGTCAATAT+TGG 0.253994 5_4:+34482977 Msa0848740:CDS
TCAACAGTGTCATTACTATT+AGG 0.287208 5_4:-34482703 None:intergenic
AAAGATCATGTTCGTGCTTT+CGG 0.314009 5_4:+34484035 Msa0848740:CDS
GGTTTGTGGTTGTGTGTTCC+AGG 0.317646 5_4:-34482757 None:intergenic
GAAAAGGACCAGAATCTCTA+AGG 0.322420 5_4:+34483739 Msa0848740:CDS
TGATGATCAGGTTTACTAAT+AGG 0.342578 5_4:-34482943 None:intergenic
TCACTCTCACTTGGCCCTCC+TGG 0.348650 5_4:+34482739 Msa0848740:CDS
TTTAATGTGGAGGATAGTTT+AGG 0.370427 5_4:-34482778 None:intergenic
TAGTAAACCTGATCATCATT+TGG 0.397302 5_4:+34482948 Msa0848740:CDS
AGCATGGAGCGAAGCCATTC+GGG 0.401651 5_4:+34483895 Msa0848740:CDS
TGGAGGATAGTTTAGGTTTG+TGG 0.408461 5_4:-34482771 None:intergenic
GGGAAACCATTTGCAGTTAG+AGG 0.410419 5_4:+34483930 Msa0848740:CDS
GAAGATCAGGAAGTATTGTT+AGG 0.411354 5_4:+34484092 Msa0848740:CDS
TTGGCTTCAGCTCCTATTCA+AGG 0.424273 5_4:+34482967 Msa0848740:CDS
AAGCATGGAGCGAAGCCATT+CGG 0.440022 5_4:+34483894 Msa0848740:CDS
CATGTTCGTGCTTTCGGTGA+TGG 0.445219 5_4:+34484041 Msa0848740:CDS
TCTCCTGCTCAAATCCTCAT+TGG 0.454551 5_4:+34483006 Msa0848740:CDS
ACCACTTTGCTCATCATCAA+TGG 0.458380 5_4:-34482866 None:intergenic
CGTCGAGAGTTGTGAAGATC+AGG 0.460359 5_4:+34484079 Msa0848740:CDS
CAACCGTTTCAATAATATGC+AGG 0.466033 5_4:+34483068 Msa0848740:CDS
CAGTCACCTCTAACTGCAAA+TGG 0.466119 5_4:-34483936 None:intergenic
AGTAGAAGAGTGATTTAATG+TGG 0.470478 5_4:-34482791 None:intergenic
TCTTCCATTTCACTCTCACT+TGG 0.472544 5_4:+34482730 Msa0848740:CDS
GCTGAAGCCAAATGATGATC+AGG 0.472827 5_4:-34482955 None:intergenic
ATCCAATATTGACCTTGAAT+AGG 0.474167 5_4:-34482979 None:intergenic
TAACCTGCATATTATTGAAA+CGG 0.475929 5_4:-34483071 None:intergenic
AGGTTCAATTGAGGATATGT+AGG 0.476900 5_4:-34482683 None:intergenic
AGTCTTAACATTGATGAAGC+TGG 0.481814 5_4:-34483771 None:intergenic
TCCTTGAGTGGTCTTGACCT+TGG 0.482145 5_4:-34483840 None:intergenic
AGGGCCAAGTGAGAGTGAAA+TGG 0.483147 5_4:-34482734 None:intergenic
GTATATATCAGATGCATATG+TGG 0.492007 5_4:+34483697 Msa0848740:intron
ATTACTATTAGGTTCAATTG+AGG 0.492152 5_4:-34482692 None:intergenic
CCTTGCTATTGCTGTGCAGA+GGG 0.509164 5_4:+34483795 Msa0848740:CDS
TATATATCAGATGCATATGT+GGG 0.512415 5_4:+34483698 Msa0848740:intron
TCCTTGCTATTGCTGTGCAG+AGG 0.519467 5_4:+34483794 Msa0848740:CDS
CAGATGCATATGTGGGGACA+TGG 0.520986 5_4:+34483705 Msa0848740:intron
CATGGATCACAATACAGAAA+AGG 0.532079 5_4:+34483723 Msa0848740:CDS
GAATATGACGAGGAATTTGA+AGG 0.538705 5_4:+34484119 Msa0848740:CDS
TCCAAGGTCAAGACCACTCA+AGG 0.543238 5_4:+34483839 Msa0848740:CDS
CACTTCAAACACACTACAAG+AGG 0.550716 5_4:+34483871 Msa0848740:CDS
TGTGGTTGTGTGTTCCAGGA+GGG 0.551458 5_4:-34482753 None:intergenic
TCACAACTCTCGACGGTGTG+TGG 0.553384 5_4:-34484071 None:intergenic
AAAAGGACCAGAATCTCTAA+GGG 0.555739 5_4:+34483740 Msa0848740:CDS
CATTTGCAGTTAGAGGTGAC+TGG 0.568578 5_4:+34483937 Msa0848740:CDS
ATGAGGATTTGAGCAGGAGA+AGG 0.568596 5_4:-34483003 None:intergenic
GGTCCAATGAGGATTTGAGC+AGG 0.576870 5_4:-34483009 None:intergenic
GGAGAATGTGTGAGCTTGCA+TGG 0.580463 5_4:-34482662 None:intergenic
CCCTCTGCACAGCAATAGCA+AGG 0.583701 5_4:-34483795 None:intergenic
CCATTCGGGTGTAGAAAGTG+TGG 0.590964 5_4:+34483909 Msa0848740:CDS
ACACACTACAAGAGGAAGCA+TGG 0.591744 5_4:+34483879 Msa0848740:CDS
AGAACAACATAGAGCATCCA+AGG 0.593445 5_4:+34483823 Msa0848740:CDS
CCACACTTTCTACACCCGAA+TGG 0.593700 5_4:-34483909 None:intergenic
TAATGATAACATCAATCGAA+TGG 0.599548 5_4:+34484169 Msa0848740:CDS
CTGATCTTCACAACTCTCGA+CGG 0.614391 5_4:-34484078 None:intergenic
ACAGTGCAAGAGAACTGTGT+TGG 0.625514 5_4:-34483030 None:intergenic
AGAAGAGTGATTTAATGTGG+AGG 0.629008 5_4:-34482788 None:intergenic
AGTGTTCTGAAATCCTTGAG+TGG 0.638667 5_4:-34483852 None:intergenic
TTGTGGTTGTGTGTTCCAGG+AGG 0.639378 5_4:-34482754 None:intergenic
AGAACTGTGTTGGTCCAATG+AGG 0.645090 5_4:-34483020 None:intergenic
GTAACAGTTGCACTTCACAT+TGG 0.647512 5_4:+34482889 Msa0848740:CDS
CATTCGGGTGTAGAAAGTGT+GGG 0.658306 5_4:+34483910 Msa0848740:CDS
ACCATTGATGATGAGCAAAG+TGG 0.669448 5_4:+34482865 Msa0848740:CDS
GTTAGGTGATGAATATGACG+AGG 0.714430 5_4:+34484109 Msa0848740:CDS
ATATATCAGATGCATATGTG+GGG 0.720271 5_4:+34483699 Msa0848740:intron
AAAGGACCAGAATCTCTAAG+GGG 0.721082 5_4:+34483741 Msa0848740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAAAAAAGTTCACTAA+AGG - chr5_4:34483324-34483343 None:intergenic 20.0%
!!! ATTGTTCATTTTTATCTTCT+AGG - chr5_4:34483571-34483590 None:intergenic 20.0%
!! CAAAAAAAAAAGTTCACTAA+AGG - chr5_4:34483324-34483343 None:intergenic 20.0%
!!! ATTGTTCATTTTTATCTTCT+AGG - chr5_4:34483571-34483590 None:intergenic 20.0%
! ATTACTATTAGGTTCAATTG+AGG - chr5_4:34482695-34482714 None:intergenic 25.0%
! TAACCTGCATATTATTGAAA+CGG - chr5_4:34483074-34483093 None:intergenic 25.0%
!!! CTAGTTCTTGACAAATTTTA+TGG - chr5_4:34483450-34483469 None:intergenic 25.0%
!!! AGGAGTATTGACAAATTTTT+TGG - chr5_4:34483487-34483506 None:intergenic 25.0%
!! AAATGACAAAATTTTGTGCT+TGG - chr5_4:34483630-34483649 None:intergenic 25.0%
! TATATATCAGATGCATATGT+GGG + chr5_4:34483698-34483717 Msa0848740:intron 25.0%
! TAATGATAACATCAATCGAA+TGG + chr5_4:34484169-34484188 Msa0848740:CDS 25.0%
! ATTACTATTAGGTTCAATTG+AGG - chr5_4:34482695-34482714 None:intergenic 25.0%
! TAACCTGCATATTATTGAAA+CGG - chr5_4:34483074-34483093 None:intergenic 25.0%
!!! CTAGTTCTTGACAAATTTTA+TGG - chr5_4:34483450-34483469 None:intergenic 25.0%
!!! AGGAGTATTGACAAATTTTT+TGG - chr5_4:34483487-34483506 None:intergenic 25.0%
!! AAATGACAAAATTTTGTGCT+TGG - chr5_4:34483630-34483649 None:intergenic 25.0%
! TATATATCAGATGCATATGT+GGG + chr5_4:34483698-34483717 Msa0848740:intron 25.0%
! TAATGATAACATCAATCGAA+TGG + chr5_4:34484169-34484188 Msa0848740:CDS 25.0%
! TCAACAGTGTCATTACTATT+AGG - chr5_4:34482706-34482725 None:intergenic 30.0%
TTTAATGTGGAGGATAGTTT+AGG - chr5_4:34482781-34482800 None:intergenic 30.0%
AGTAGAAGAGTGATTTAATG+TGG - chr5_4:34482794-34482813 None:intergenic 30.0%
! CAATGGTAAAGTTTTGCTTT+TGG - chr5_4:34482852-34482871 None:intergenic 30.0%
! TGTTGTGTTTTGTGAAATGT+TGG - chr5_4:34482923-34482942 None:intergenic 30.0%
TGATGATCAGGTTTACTAAT+AGG - chr5_4:34482946-34482965 None:intergenic 30.0%
TAGTAAACCTGATCATCATT+TGG + chr5_4:34482948-34482967 Msa0848740:CDS 30.0%
ATCCAATATTGACCTTGAAT+AGG - chr5_4:34482982-34483001 None:intergenic 30.0%
AACGATTCAAGATGGTATTA+GGG - chr5_4:34483380-34483399 None:intergenic 30.0%
TAACGATTCAAGATGGTATT+AGG - chr5_4:34483381-34483400 None:intergenic 30.0%
! AAAGTGTTAACGATTCAAGA+TGG - chr5_4:34483388-34483407 None:intergenic 30.0%
ATGTCCTAATGCATACTATA+TGG + chr5_4:34483510-34483529 Msa0848740:intron 30.0%
TCTTCCATATAGTATGCATT+AGG - chr5_4:34483517-34483536 None:intergenic 30.0%
GTATATATCAGATGCATATG+TGG + chr5_4:34483697-34483716 Msa0848740:intron 30.0%
ATATATCAGATGCATATGTG+GGG + chr5_4:34483699-34483718 Msa0848740:intron 30.0%
AGAACACATGAGAAAAATTG+TGG + chr5_4:34483960-34483979 Msa0848740:CDS 30.0%
AGAAAAATTGTGGAAAGCTT+TGG + chr5_4:34483970-34483989 Msa0848740:CDS 30.0%
! AACATCAATCGAATGGTTTT+CGG + chr5_4:34484176-34484195 Msa0848740:CDS 30.0%
! TCAACAGTGTCATTACTATT+AGG - chr5_4:34482706-34482725 None:intergenic 30.0%
TTTAATGTGGAGGATAGTTT+AGG - chr5_4:34482781-34482800 None:intergenic 30.0%
AGTAGAAGAGTGATTTAATG+TGG - chr5_4:34482794-34482813 None:intergenic 30.0%
! CAATGGTAAAGTTTTGCTTT+TGG - chr5_4:34482852-34482871 None:intergenic 30.0%
! TGTTGTGTTTTGTGAAATGT+TGG - chr5_4:34482923-34482942 None:intergenic 30.0%
TGATGATCAGGTTTACTAAT+AGG - chr5_4:34482946-34482965 None:intergenic 30.0%
TAGTAAACCTGATCATCATT+TGG + chr5_4:34482948-34482967 Msa0848740:CDS 30.0%
ATCCAATATTGACCTTGAAT+AGG - chr5_4:34482982-34483001 None:intergenic 30.0%
AACGATTCAAGATGGTATTA+GGG - chr5_4:34483380-34483399 None:intergenic 30.0%
TAACGATTCAAGATGGTATT+AGG - chr5_4:34483381-34483400 None:intergenic 30.0%
! AAAGTGTTAACGATTCAAGA+TGG - chr5_4:34483388-34483407 None:intergenic 30.0%
ATGTCCTAATGCATACTATA+TGG + chr5_4:34483510-34483529 Msa0848740:intron 30.0%
TCTTCCATATAGTATGCATT+AGG - chr5_4:34483517-34483536 None:intergenic 30.0%
GTATATATCAGATGCATATG+TGG + chr5_4:34483697-34483716 Msa0848740:intron 30.0%
ATATATCAGATGCATATGTG+GGG + chr5_4:34483699-34483718 Msa0848740:intron 30.0%
AGAACACATGAGAAAAATTG+TGG + chr5_4:34483960-34483979 Msa0848740:CDS 30.0%
AGAAAAATTGTGGAAAGCTT+TGG + chr5_4:34483970-34483989 Msa0848740:CDS 30.0%
! AACATCAATCGAATGGTTTT+CGG + chr5_4:34484176-34484195 Msa0848740:CDS 30.0%
AGGTTCAATTGAGGATATGT+AGG - chr5_4:34482686-34482705 None:intergenic 35.0%
AGAAGAGTGATTTAATGTGG+AGG - chr5_4:34482791-34482810 None:intergenic 35.0%
!! TGGTAAAGTTTTGCTTTTGG+TGG - chr5_4:34482849-34482868 None:intergenic 35.0%
! GTGTTTTGTGAAATGTTGGT+TGG - chr5_4:34482919-34482938 None:intergenic 35.0%
CTCCTATTCAAGGTCAATAT+TGG + chr5_4:34482977-34482996 Msa0848740:CDS 35.0%
CAACCGTTTCAATAATATGC+AGG + chr5_4:34483068-34483087 Msa0848740:CDS 35.0%
TGTATCTTGAACTACTAGCT+AGG - chr5_4:34483151-34483170 None:intergenic 35.0%
AGTATGCATTAGGACATTGA+AGG - chr5_4:34483507-34483526 None:intergenic 35.0%
CATGGATCACAATACAGAAA+AGG + chr5_4:34483723-34483742 Msa0848740:CDS 35.0%
AAAAGGACCAGAATCTCTAA+GGG + chr5_4:34483740-34483759 Msa0848740:CDS 35.0%
AGTCTTAACATTGATGAAGC+TGG - chr5_4:34483774-34483793 None:intergenic 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr5_4:34483984-34484003 Msa0848740:CDS 35.0%
AAAGATCATGTTCGTGCTTT+CGG + chr5_4:34484035-34484054 Msa0848740:CDS 35.0%
!! GAAGATCAGGAAGTATTGTT+AGG + chr5_4:34484092-34484111 Msa0848740:CDS 35.0%
GAATATGACGAGGAATTTGA+AGG + chr5_4:34484119-34484138 Msa0848740:CDS 35.0%
AGGTTCAATTGAGGATATGT+AGG - chr5_4:34482686-34482705 None:intergenic 35.0%
AGAAGAGTGATTTAATGTGG+AGG - chr5_4:34482791-34482810 None:intergenic 35.0%
!! TGGTAAAGTTTTGCTTTTGG+TGG - chr5_4:34482849-34482868 None:intergenic 35.0%
! GTGTTTTGTGAAATGTTGGT+TGG - chr5_4:34482919-34482938 None:intergenic 35.0%
CTCCTATTCAAGGTCAATAT+TGG + chr5_4:34482977-34482996 Msa0848740:CDS 35.0%
CAACCGTTTCAATAATATGC+AGG + chr5_4:34483068-34483087 Msa0848740:CDS 35.0%
TGTATCTTGAACTACTAGCT+AGG - chr5_4:34483151-34483170 None:intergenic 35.0%
AGTATGCATTAGGACATTGA+AGG - chr5_4:34483507-34483526 None:intergenic 35.0%
CATGGATCACAATACAGAAA+AGG + chr5_4:34483723-34483742 Msa0848740:CDS 35.0%
AAAAGGACCAGAATCTCTAA+GGG + chr5_4:34483740-34483759 Msa0848740:CDS 35.0%
AGTCTTAACATTGATGAAGC+TGG - chr5_4:34483774-34483793 None:intergenic 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr5_4:34483984-34484003 Msa0848740:CDS 35.0%
AAAGATCATGTTCGTGCTTT+CGG + chr5_4:34484035-34484054 Msa0848740:CDS 35.0%
!! GAAGATCAGGAAGTATTGTT+AGG + chr5_4:34484092-34484111 Msa0848740:CDS 35.0%
GAATATGACGAGGAATTTGA+AGG + chr5_4:34484119-34484138 Msa0848740:CDS 35.0%
TCTTCCATTTCACTCTCACT+TGG + chr5_4:34482730-34482749 Msa0848740:CDS 40.0%
! TGGAGGATAGTTTAGGTTTG+TGG - chr5_4:34482774-34482793 None:intergenic 40.0%
!! ACCATTGATGATGAGCAAAG+TGG + chr5_4:34482865-34482884 Msa0848740:CDS 40.0%
ACCACTTTGCTCATCATCAA+TGG - chr5_4:34482869-34482888 None:intergenic 40.0%
GTAACAGTTGCACTTCACAT+TGG + chr5_4:34482889-34482908 Msa0848740:CDS 40.0%
AGCTAGGCAATAGACAGTTT+AGG - chr5_4:34483135-34483154 None:intergenic 40.0%
GAAAAGGACCAGAATCTCTA+AGG + chr5_4:34483739-34483758 Msa0848740:CDS 40.0%
AAAGGACCAGAATCTCTAAG+GGG + chr5_4:34483741-34483760 Msa0848740:CDS 40.0%
TTTACTCCCCTTAGAGATTC+TGG - chr5_4:34483750-34483769 None:intergenic 40.0%
! AGAACAACATAGAGCATCCA+AGG + chr5_4:34483823-34483842 Msa0848740:CDS 40.0%
!! AGTGTTCTGAAATCCTTGAG+TGG - chr5_4:34483855-34483874 None:intergenic 40.0%
CACTTCAAACACACTACAAG+AGG + chr5_4:34483871-34483890 Msa0848740:CDS 40.0%
GTTAGGTGATGAATATGACG+AGG + chr5_4:34484109-34484128 Msa0848740:CDS 40.0%
TCTTCCATTTCACTCTCACT+TGG + chr5_4:34482730-34482749 Msa0848740:CDS 40.0%
! TGGAGGATAGTTTAGGTTTG+TGG - chr5_4:34482774-34482793 None:intergenic 40.0%
!! ACCATTGATGATGAGCAAAG+TGG + chr5_4:34482865-34482884 Msa0848740:CDS 40.0%
ACCACTTTGCTCATCATCAA+TGG - chr5_4:34482869-34482888 None:intergenic 40.0%
GTAACAGTTGCACTTCACAT+TGG + chr5_4:34482889-34482908 Msa0848740:CDS 40.0%
AGCTAGGCAATAGACAGTTT+AGG - chr5_4:34483135-34483154 None:intergenic 40.0%
GAAAAGGACCAGAATCTCTA+AGG + chr5_4:34483739-34483758 Msa0848740:CDS 40.0%
AAAGGACCAGAATCTCTAAG+GGG + chr5_4:34483741-34483760 Msa0848740:CDS 40.0%
TTTACTCCCCTTAGAGATTC+TGG - chr5_4:34483750-34483769 None:intergenic 40.0%
! AGAACAACATAGAGCATCCA+AGG + chr5_4:34483823-34483842 Msa0848740:CDS 40.0%
!! AGTGTTCTGAAATCCTTGAG+TGG - chr5_4:34483855-34483874 None:intergenic 40.0%
CACTTCAAACACACTACAAG+AGG + chr5_4:34483871-34483890 Msa0848740:CDS 40.0%
GTTAGGTGATGAATATGACG+AGG + chr5_4:34484109-34484128 Msa0848740:CDS 40.0%
GCTGAAGCCAAATGATGATC+AGG - chr5_4:34482958-34482977 None:intergenic 45.0%
TTGGCTTCAGCTCCTATTCA+AGG + chr5_4:34482967-34482986 Msa0848740:CDS 45.0%
ATGAGGATTTGAGCAGGAGA+AGG - chr5_4:34483006-34483025 None:intergenic 45.0%
TCTCCTGCTCAAATCCTCAT+TGG + chr5_4:34483006-34483025 Msa0848740:CDS 45.0%
! AGAACTGTGTTGGTCCAATG+AGG - chr5_4:34483023-34483042 None:intergenic 45.0%
ACAGTGCAAGAGAACTGTGT+TGG - chr5_4:34483033-34483052 None:intergenic 45.0%
ACACACTACAAGAGGAAGCA+TGG + chr5_4:34483879-34483898 Msa0848740:CDS 45.0%
! CATTCGGGTGTAGAAAGTGT+GGG + chr5_4:34483910-34483929 Msa0848740:CDS 45.0%
GGGAAACCATTTGCAGTTAG+AGG + chr5_4:34483930-34483949 Msa0848740:CDS 45.0%
CAGTCACCTCTAACTGCAAA+TGG - chr5_4:34483939-34483958 None:intergenic 45.0%
CATTTGCAGTTAGAGGTGAC+TGG + chr5_4:34483937-34483956 Msa0848740:CDS 45.0%
CTGATCTTCACAACTCTCGA+CGG - chr5_4:34484081-34484100 None:intergenic 45.0%
GCTGAAGCCAAATGATGATC+AGG - chr5_4:34482958-34482977 None:intergenic 45.0%
TTGGCTTCAGCTCCTATTCA+AGG + chr5_4:34482967-34482986 Msa0848740:CDS 45.0%
ATGAGGATTTGAGCAGGAGA+AGG - chr5_4:34483006-34483025 None:intergenic 45.0%
TCTCCTGCTCAAATCCTCAT+TGG + chr5_4:34483006-34483025 Msa0848740:CDS 45.0%
! AGAACTGTGTTGGTCCAATG+AGG - chr5_4:34483023-34483042 None:intergenic 45.0%
ACAGTGCAAGAGAACTGTGT+TGG - chr5_4:34483033-34483052 None:intergenic 45.0%
ACACACTACAAGAGGAAGCA+TGG + chr5_4:34483879-34483898 Msa0848740:CDS 45.0%
! CATTCGGGTGTAGAAAGTGT+GGG + chr5_4:34483910-34483929 Msa0848740:CDS 45.0%
GGGAAACCATTTGCAGTTAG+AGG + chr5_4:34483930-34483949 Msa0848740:CDS 45.0%
CAGTCACCTCTAACTGCAAA+TGG - chr5_4:34483939-34483958 None:intergenic 45.0%
CATTTGCAGTTAGAGGTGAC+TGG + chr5_4:34483937-34483956 Msa0848740:CDS 45.0%
CTGATCTTCACAACTCTCGA+CGG - chr5_4:34484081-34484100 None:intergenic 45.0%
!!! TTTTTATAAATAAATATTAG+TGG + chr5_4:34483409-34483428 Msa0848740:intron 5.0%
!!! TTTTTATAAATAAATATTAG+TGG + chr5_4:34483409-34483428 Msa0848740:intron 5.0%
AGGGCCAAGTGAGAGTGAAA+TGG - chr5_4:34482737-34482756 None:intergenic 50.0%
TGTGGTTGTGTGTTCCAGGA+GGG - chr5_4:34482756-34482775 None:intergenic 50.0%
TTGTGGTTGTGTGTTCCAGG+AGG - chr5_4:34482757-34482776 None:intergenic 50.0%
GGTTTGTGGTTGTGTGTTCC+AGG - chr5_4:34482760-34482779 None:intergenic 50.0%
GGTCCAATGAGGATTTGAGC+AGG - chr5_4:34483012-34483031 None:intergenic 50.0%
CAGATGCATATGTGGGGACA+TGG + chr5_4:34483705-34483724 Msa0848740:intron 50.0%
!! TCCTTGCTATTGCTGTGCAG+AGG + chr5_4:34483794-34483813 Msa0848740:CDS 50.0%
!! CCTTGCTATTGCTGTGCAGA+GGG + chr5_4:34483795-34483814 Msa0848740:CDS 50.0%
TCCAAGGTCAAGACCACTCA+AGG + chr5_4:34483839-34483858 Msa0848740:CDS 50.0%
! TCCTTGAGTGGTCTTGACCT+TGG - chr5_4:34483843-34483862 None:intergenic 50.0%
AAGCATGGAGCGAAGCCATT+CGG + chr5_4:34483894-34483913 Msa0848740:CDS 50.0%
CCACACTTTCTACACCCGAA+TGG - chr5_4:34483912-34483931 None:intergenic 50.0%
CCATTCGGGTGTAGAAAGTG+TGG + chr5_4:34483909-34483928 Msa0848740:CDS 50.0%
CATGTTCGTGCTTTCGGTGA+TGG + chr5_4:34484041-34484060 Msa0848740:CDS 50.0%
CGTCGAGAGTTGTGAAGATC+AGG + chr5_4:34484079-34484098 Msa0848740:CDS 50.0%
AGGGCCAAGTGAGAGTGAAA+TGG - chr5_4:34482737-34482756 None:intergenic 50.0%
TGTGGTTGTGTGTTCCAGGA+GGG - chr5_4:34482756-34482775 None:intergenic 50.0%
TTGTGGTTGTGTGTTCCAGG+AGG - chr5_4:34482757-34482776 None:intergenic 50.0%
GGTTTGTGGTTGTGTGTTCC+AGG - chr5_4:34482760-34482779 None:intergenic 50.0%
GGTCCAATGAGGATTTGAGC+AGG - chr5_4:34483012-34483031 None:intergenic 50.0%
CAGATGCATATGTGGGGACA+TGG + chr5_4:34483705-34483724 Msa0848740:intron 50.0%
!! TCCTTGCTATTGCTGTGCAG+AGG + chr5_4:34483794-34483813 Msa0848740:CDS 50.0%
!! CCTTGCTATTGCTGTGCAGA+GGG + chr5_4:34483795-34483814 Msa0848740:CDS 50.0%
TCCAAGGTCAAGACCACTCA+AGG + chr5_4:34483839-34483858 Msa0848740:CDS 50.0%
! TCCTTGAGTGGTCTTGACCT+TGG - chr5_4:34483843-34483862 None:intergenic 50.0%
AAGCATGGAGCGAAGCCATT+CGG + chr5_4:34483894-34483913 Msa0848740:CDS 50.0%
CCACACTTTCTACACCCGAA+TGG - chr5_4:34483912-34483931 None:intergenic 50.0%
CCATTCGGGTGTAGAAAGTG+TGG + chr5_4:34483909-34483928 Msa0848740:CDS 50.0%
CATGTTCGTGCTTTCGGTGA+TGG + chr5_4:34484041-34484060 Msa0848740:CDS 50.0%
CGTCGAGAGTTGTGAAGATC+AGG + chr5_4:34484079-34484098 Msa0848740:CDS 50.0%
CCCTCTGCACAGCAATAGCA+AGG - chr5_4:34483798-34483817 None:intergenic 55.0%
AGCATGGAGCGAAGCCATTC+GGG + chr5_4:34483895-34483914 Msa0848740:CDS 55.0%
TCACAACTCTCGACGGTGTG+TGG - chr5_4:34484074-34484093 None:intergenic 55.0%
CCCTCTGCACAGCAATAGCA+AGG - chr5_4:34483798-34483817 None:intergenic 55.0%
AGCATGGAGCGAAGCCATTC+GGG + chr5_4:34483895-34483914 Msa0848740:CDS 55.0%
TCACAACTCTCGACGGTGTG+TGG - chr5_4:34484074-34484093 None:intergenic 55.0%
TCACTCTCACTTGGCCCTCC+TGG + chr5_4:34482739-34482758 Msa0848740:CDS 60.0%
TCACTCTCACTTGGCCCTCC+TGG + chr5_4:34482739-34482758 Msa0848740:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 34482664 34484205 34482664 ID=Msa0848740;Name=Msa0848740
chr5_4 mRNA 34482664 34484205 34482664 ID=Msa0848740-mRNA-1;Parent=Msa0848740;Name=Msa0848740-mRNA-1;_AED=0.06;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|307
chr5_4 exon 34482664 34483089 34482664 ID=Msa0848740-mRNA-1:exon:11655;Parent=Msa0848740-mRNA-1
chr5_4 exon 34483708 34484205 34483708 ID=Msa0848740-mRNA-1:exon:11656;Parent=Msa0848740-mRNA-1
chr5_4 CDS 34482664 34483089 34482664 ID=Msa0848740-mRNA-1:cds;Parent=Msa0848740-mRNA-1
chr5_4 CDS 34483708 34484205 34483708 ID=Msa0848740-mRNA-1:cds;Parent=Msa0848740-mRNA-1
Gene Sequence

>Msa0848740

ATGCAAGCTCACACATTCTCCTACATATCCTCAATTGAACCTAATAGTAATGACACTGTTGATGAGTCTTCCATTTCACTCTCACTTGGCCCTCCTGGAACACACAACCACAAACCTAAACTATCCTCCACATTAAATCACTCTTCTACTACTCTTCATCATCATCATCATAATCATAATCACCACCAAAAGCAAAACTTTACCATTGATGATGAGCAAAGTGGTGTAACAGTTGCACTTCACATTGGTCTTCCAACCAACATTTCACAAAACACAACACCTATTAGTAAACCTGATCATCATTTGGCTTCAGCTCCTATTCAAGGTCAATATTGGATTCCTTCTCCTGCTCAAATCCTCATTGGACCAACACAGTTCTCTTGCACTGTTTGCAATAAAATGTTCAACCGTTTCAATAATATGCAGATGCATATGTGGGGACATGGATCACAATACAGAAAAGGACCAGAATCTCTAAGGGGAGTAAAACCAGCTTCATCAATGTTAAGACTTCCTTGCTATTGCTGTGCAGAGGGATGCAAGAACAACATAGAGCATCCAAGGTCAAGACCACTCAAGGATTTCAGAACACTTCAAACACACTACAAGAGGAAGCATGGAGCGAAGCCATTCGGGTGTAGAAAGTGTGGGAAACCATTTGCAGTTAGAGGTGACTGGAGAACACATGAGAAAAATTGTGGAAAGCTTTGGTTTTGTATCTGTGGATCTGATTTCAAACACAAGAGATCTCTTAAAGATCATGTTCGTGCTTTCGGTGATGGTCATGCTCCACACACCGTCGAGAGTTGTGAAGATCAGGAAGTATTGTTAGGTGATGAATATGACGAGGAATTTGAAGGAGATGAAAATTATGAAGACGATGATGATAATGATAACATCAATCGAATGGTTTTCGGTTTTTAA
Protein sequence

>Msa0848740

MQAHTFSYISSIEPNSNDTVDESSISLSLGPPGTHNHKPKLSSTLNHSSTTLHHHHHNHNHHQKQNFTIDDEQSGVTVALHIGLPTNISQNTTPISKPDHHLASAPIQGQYWIPSPAQILIGPTQFSCTVCNKMFNRFNNMQMHMWGHGSQYRKGPESLRGVKPASSMLRLPCYCCAEGCKNNIEHPRSRPLKDFRTLQTHYKRKHGAKPFGCRKCGKPFAVRGDWRTHEKNCGKLWFCICGSDFKHKRSLKDHVRAFGDGHAPHTVESCEDQEVLLGDEYDEEFEGDENYEDDDDNDNINRMVFGF*