Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0849170 | XP_039688063.1 | 63.063 | 111 | 5 | 1 | 1 | 75 | 51 | 161 | 4.58e-36 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0849170 | A0A072U9Z0 | 58.000 | 100 | 6 | 1 | 1 | 64 | 74 | 173 | 4.30e-28 | 109 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0849170 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTACCATCCTTCTTATT+TGG | 0.212526 | 5_4:-35640467 | None:intergenic |
| TTTAATTCATCATCACAATT+TGG | 0.253101 | 5_4:-35640281 | None:intergenic |
| GGGTTATTTGCCAAATAAGA+AGG | 0.490320 | 5_4:+35640457 | Msa0849170:CDS |
| CTGTCAAGGTTAGAAGAAGA+AGG | 0.491761 | 5_4:+35640504 | Msa0849170:CDS |
| GCCAAATAAGAAGGATGGTA+AGG | 0.493986 | 5_4:+35640466 | Msa0849170:CDS |
| GATGAATTAAAGCCTACGAT+TGG | 0.535839 | 5_4:+35640293 | Msa0849170:CDS |
| AGAAGGGCTTTGACAAGAGA+AGG | 0.567487 | 5_4:+35640521 | Msa0849170:CDS |
| TATTTGCCAAATAAGAAGGA+TGG | 0.592394 | 5_4:+35640461 | Msa0849170:CDS |
| GCAAAGTGAATCTACTGTCA+AGG | 0.595204 | 5_4:+35640490 | Msa0849170:CDS |
| TGTCAAGGTTAGAAGAAGAA+GGG | 0.596214 | 5_4:+35640505 | Msa0849170:CDS |
| TCAAATACTTGTCCAATCGT+AGG | 0.603614 | 5_4:-35640305 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATTCATCATCACAATT+TGG | - | chr5_4:35640284-35640303 | None:intergenic | 20.0% |
| !! | TCATCGACTATAGATAAAAA+TGG | + | chr5_4:35640398-35640417 | Msa0849170:intron | 25.0% |
| !!! | ATGCAAATGTTGTTTTCAAA+TGG | + | chr5_4:35640549-35640568 | Msa0849170:CDS | 25.0% |
| !!! | TGCAAATGTTGTTTTCAAAT+GGG | + | chr5_4:35640550-35640569 | Msa0849170:CDS | 25.0% |
| GTATTTGATACGTTAGCATA+AGG | + | chr5_4:35640320-35640339 | Msa0849170:intron | 30.0% | |
| !! | TATTTTGTTTGCTCGAAAGA+GGG | + | chr5_4:35640437-35640456 | Msa0849170:CDS | 30.0% |
| TATTTGCCAAATAAGAAGGA+TGG | + | chr5_4:35640461-35640480 | Msa0849170:CDS | 30.0% | |
| !!! | AAATGTTGTTTTCAAATGGG+TGG | + | chr5_4:35640553-35640572 | Msa0849170:CDS | 30.0% |
| !!! | AATGTTGTTTTCAAATGGGT+GGG | + | chr5_4:35640554-35640573 | Msa0849170:CDS | 30.0% |
| GATGAATTAAAGCCTACGAT+TGG | + | chr5_4:35640293-35640312 | Msa0849170:CDS | 35.0% | |
| TCAAATACTTGTCCAATCGT+AGG | - | chr5_4:35640308-35640327 | None:intergenic | 35.0% | |
| ATAAAAATGGCATGTAGCGT+TGG | + | chr5_4:35640411-35640430 | Msa0849170:intron | 35.0% | |
| !! | GTATTTTGTTTGCTCGAAAG+AGG | + | chr5_4:35640436-35640455 | Msa0849170:CDS | 35.0% |
| GGGTTATTTGCCAAATAAGA+AGG | + | chr5_4:35640457-35640476 | Msa0849170:CDS | 35.0% | |
| TCCTTACCATCCTTCTTATT+TGG | - | chr5_4:35640470-35640489 | None:intergenic | 35.0% | |
| TGTCAAGGTTAGAAGAAGAA+GGG | + | chr5_4:35640505-35640524 | Msa0849170:CDS | 35.0% | |
| !!! | ATGTTGTTTTCAAATGGGTG+GGG | + | chr5_4:35640555-35640574 | Msa0849170:CDS | 35.0% |
| GAAAAATACGCACTCTCTGT+CGG | + | chr5_4:35640356-35640375 | Msa0849170:intron | 40.0% | |
| GCCAAATAAGAAGGATGGTA+AGG | + | chr5_4:35640466-35640485 | Msa0849170:CDS | 40.0% | |
| GCAAAGTGAATCTACTGTCA+AGG | + | chr5_4:35640490-35640509 | Msa0849170:CDS | 40.0% | |
| CTGTCAAGGTTAGAAGAAGA+AGG | + | chr5_4:35640504-35640523 | Msa0849170:CDS | 40.0% | |
| !!! | TGTTGTTTTCAAATGGGTGG+GGG | + | chr5_4:35640556-35640575 | Msa0849170:CDS | 40.0% |
| ! | AGAAGGGCTTTGACAAGAGA+AGG | + | chr5_4:35640521-35640540 | Msa0849170:CDS | 45.0% |
| ! | GTTTTCAAATGGGTGGGGGA+AGG | + | chr5_4:35640560-35640579 | Msa0849170:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 35640266 | 35640601 | 35640266 | ID=Msa0849170;Name=Msa0849170 |
| chr5_4 | mRNA | 35640266 | 35640601 | 35640266 | ID=Msa0849170-mRNA-1;Parent=Msa0849170;Name=Msa0849170-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|75 |
| chr5_4 | exon | 35640266 | 35640319 | 35640266 | ID=Msa0849170-mRNA-1:exon:11895;Parent=Msa0849170-mRNA-1 |
| chr5_4 | exon | 35640428 | 35640601 | 35640428 | ID=Msa0849170-mRNA-1:exon:11896;Parent=Msa0849170-mRNA-1 |
| chr5_4 | CDS | 35640266 | 35640319 | 35640266 | ID=Msa0849170-mRNA-1:cds;Parent=Msa0849170-mRNA-1 |
| chr5_4 | CDS | 35640428 | 35640601 | 35640428 | ID=Msa0849170-mRNA-1:cds;Parent=Msa0849170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
