AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0854670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0854670 MtrunA17_Chr5g0432071 91.469 211 18 0 56 266 1 211 2.31e-144 403
Msa0854670 MtrunA17_Chr5g0431931 66.923 130 41 2 45 173 26 154 6.77e-54 171
Msa0854670 MtrunA17_Chr5g0444671 66.929 127 18 3 1 126 62 165 3.83e-46 152
Msa0854670 MtrunA17_Chr5g0432011 71.111 90 25 1 56 144 1 90 1.70e-36 125
Msa0854670 MtrunA17_Chr5g0432041 52.713 129 61 0 1 129 66 194 6.99e-32 124
Msa0854670 MtrunA17_Chr5g0432161 49.306 144 61 5 131 266 214 353 3.63e-31 121
Msa0854670 MtrunA17_Chr5g0432161 51.613 62 29 1 159 219 421 482 2.17e-14 72.8
Msa0854670 MtrunA17_Chr5g0431741 38.462 208 115 5 1 200 65 267 6.31e-30 118
Msa0854670 MtrunA17_Chr5g0431921 50.758 132 60 3 1 128 65 195 9.85e-30 112
Msa0854670 MtrunA17_Chr5g0431981 43.373 166 84 2 1 159 62 224 1.98e-29 114
Msa0854670 MtrunA17_Chr5g0432181 48.227 141 63 2 1 136 65 200 3.81e-29 112
Msa0854670 MtrunA17_Chr5g0431811 49.655 145 62 3 1 134 65 209 2.06e-28 114
Msa0854670 MtrunA17_Chr3g0106491 47.761 134 64 3 134 266 173 301 5.43e-28 112
Msa0854670 MtrunA17_Chr3g0106491 43.590 78 40 2 159 235 365 439 1.25e-12 67.4
Msa0854670 MtrunA17_Chr5g0432031 46.715 137 58 4 7 141 84 207 8.67e-27 104
Msa0854670 MtrunA17_Chr3g0104851 50.435 115 53 1 1 111 79 193 1.62e-26 108
Msa0854670 MtrunA17_Chr5g0431761 52.252 111 53 0 1 111 65 175 2.14e-26 107
Msa0854670 MtrunA17_Chr7g0231451 38.994 159 94 2 1 157 62 219 8.75e-26 103
Msa0854670 MtrunA17_Chr7g0243891 55.814 86 34 2 141 225 200 282 6.70e-25 103
Msa0854670 MtrunA17_Chr7g0243891 48.387 62 31 1 159 219 374 435 8.24e-13 67.8
Msa0854670 MtrunA17_Chr5g0432051 43.796 137 63 3 7 141 82 206 1.66e-24 98.6
Msa0854670 MtrunA17_Chr5g0444661 58.889 90 37 0 7 96 72 161 3.52e-24 100
Msa0854670 MtrunA17_Chr5g0432091 55.238 105 47 0 7 111 71 175 6.48e-24 100
Msa0854670 MtrunA17_Chr7g0263531 64.706 68 23 1 160 227 190 256 1.00e-23 99.4
Msa0854670 MtrunA17_Chr7g0263531 49.153 59 29 1 160 217 362 420 5.38e-11 62.4
Msa0854670 MtrunA17_Chr6g0482231 39.103 156 89 3 1 154 156 307 4.50e-23 96.7
Msa0854670 MtrunA17_Chr5g0431991 34.483 232 126 5 1 228 65 274 4.74e-23 97.4
Msa0854670 MtrunA17_Chr7g0249311 64.062 64 23 0 159 222 179 242 8.05e-22 94.7
Msa0854670 MtrunA17_Chr7g0249311 46.774 62 32 1 159 219 392 453 5.08e-12 65.9
Msa0854670 MtrunA17_Chr4g0004911 53.165 79 35 2 154 231 179 256 2.22e-21 93.2
Msa0854670 MtrunA17_Chr4g0004911 48.333 60 30 1 159 217 336 395 1.11e-11 64.3
Msa0854670 MtrunA17_Chr5g0431801 41.353 133 74 2 5 133 70 202 5.56e-21 90.1
Msa0854670 MtrunA17_Chr3g0079691 60.938 64 25 0 159 222 280 343 7.09e-21 92.0
Msa0854670 MtrunA17_Chr3g0079691 48.387 62 31 1 159 219 495 556 2.60e-12 66.6
Msa0854670 MtrunA17_Chr5g0431821 42.520 127 69 2 7 129 72 198 1.94e-20 90.1
Msa0854670 MtrunA17_Chr6g0482191 41.129 124 72 1 1 123 525 648 2.79e-20 90.1
Msa0854670 MtrunA17_Chr5g0445311 57.353 68 29 0 160 227 201 268 5.01e-20 89.4
Msa0854670 MtrunA17_Chr5g0445311 46.774 62 32 1 159 219 364 425 1.02e-12 67.8
Msa0854670 MtrunA17_Chr3g0101131 57.812 64 27 0 159 222 243 306 6.67e-20 89.0
Msa0854670 MtrunA17_Chr3g0101131 48.387 62 31 1 159 219 415 476 1.92e-13 69.7
Msa0854670 MtrunA17_Chr7g0218081 51.724 87 40 1 142 226 211 297 7.80e-20 88.6
Msa0854670 MtrunA17_Chr7g0218081 41.837 98 46 4 159 249 410 503 2.71e-13 69.3
Msa0854670 MtrunA17_Chr6g0482151 40.000 110 66 0 8 117 50 159 9.01e-20 83.6
Msa0854670 MtrunA17_Chr7g0249301 57.812 64 27 0 159 222 62 125 3.45e-19 85.9
Msa0854670 MtrunA17_Chr7g0249301 46.774 62 32 1 159 219 292 353 1.11e-11 64.3
Msa0854670 MtrunA17_Chr5g0418191 61.017 59 23 0 159 217 151 209 6.55e-19 85.5
Msa0854670 MtrunA17_Chr5g0418191 48.387 62 31 1 159 219 320 381 3.01e-13 68.9
Msa0854670 MtrunA17_Chr5g0431851 46.602 103 54 1 1 102 65 167 5.47e-18 81.6
Msa0854670 MtrunA17_Chr1g0188941 50.000 62 30 1 159 219 51 112 1.06e-16 73.9
Msa0854670 MtrunA17_Chr4g0042831 41.748 103 53 3 159 260 145 241 1.16e-16 79.0
Msa0854670 MtrunA17_Chr4g0042831 50.000 62 30 1 159 219 278 339 1.23e-13 70.1
Msa0854670 MtrunA17_Chr6g0468181 64.151 53 18 1 175 227 18 69 1.74e-15 70.9
Msa0854670 MtrunA17_Chr5g0447711 34.921 126 70 2 108 221 20 145 1.47e-13 69.3
Msa0854670 MtrunA17_Chr5g0447701 29.885 174 100 3 89 240 1 174 1.59e-13 69.7
Msa0854670 MtrunA17_Chr5g0447701 43.333 60 33 1 160 218 253 312 9.17e-12 64.7
Msa0854670 MtrunA17_Chr4g0051881 41.935 93 47 3 159 250 178 264 1.80e-13 69.3
Msa0854670 MtrunA17_Chr3g0136961 38.462 91 49 5 159 242 138 228 2.51e-13 68.6
Msa0854670 MtrunA17_Chr1g0194481 49.206 63 31 1 159 220 158 220 3.29e-13 68.6
Msa0854670 MtrunA17_Chr5g0443131 48.387 62 31 1 159 219 157 218 5.67e-13 67.8
Msa0854670 MtrunA17_Chr2g0333851 34.118 85 53 3 159 240 169 253 1.75e-12 66.2
Msa0854670 MtrunA17_Chr2g0294231 46.032 63 33 1 159 220 203 265 3.07e-12 65.9
Msa0854670 MtrunA17_Chr7g0270191 46.154 78 38 2 159 235 145 219 3.23e-12 65.5
Msa0854670 MtrunA17_Chr3g0129361 37.662 77 46 2 159 234 144 219 9.65e-12 63.2
Msa0854670 MtrunA17_Chr8g0383351 47.368 57 29 1 159 214 160 216 1.19e-11 63.2
Msa0854670 MtrunA17_Chr8g0335811 50.877 57 27 1 159 214 154 210 1.35e-11 62.8
Msa0854670 MtrunA17_Chr4g0068541 46.667 60 31 1 159 217 208 267 2.54e-11 63.2
Msa0854670 MtrunA17_Chr8g0386241 42.105 57 32 1 159 214 103 159 9.52e-11 59.7
Msa0854670 MtrunA17_Chr5g0431951 47.143 70 36 1 1 70 79 147 9.87e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0854670 AT2G38470.1 66.102 59 20 0 159 217 184 242 2.79e-23 99.0
Msa0854670 AT2G38470.1 48.387 62 31 1 159 219 362 423 8.95e-12 65.1
Msa0854670 AT5G07100.2 60.000 70 26 2 159 227 24 92 2.42e-22 92.4
Msa0854670 AT5G07100.2 49.180 61 30 1 159 218 141 201 6.42e-13 66.6
Msa0854670 AT5G07100.4 60.000 70 26 2 159 227 24 92 2.42e-22 92.4
Msa0854670 AT5G07100.4 49.180 61 30 1 159 218 141 201 6.42e-13 66.6
Msa0854670 AT5G07100.5 60.000 70 26 2 159 227 24 92 2.42e-22 92.4
Msa0854670 AT5G07100.5 49.180 61 30 1 159 218 141 201 6.42e-13 66.6
Msa0854670 AT5G07100.3 60.000 70 26 2 159 227 24 92 2.42e-22 92.4
Msa0854670 AT5G07100.3 49.180 61 30 1 159 218 141 201 6.42e-13 66.6
Msa0854670 AT5G07100.1 60.000 70 26 2 159 227 117 185 2.72e-22 94.0
Msa0854670 AT5G07100.1 48.387 62 31 1 159 219 234 295 4.41e-13 68.2
Msa0854670 AT5G56270.1 60.938 64 25 0 159 222 273 336 1.30e-21 94.4
Msa0854670 AT5G56270.1 50.000 62 30 1 159 219 487 548 9.86e-14 71.2
Msa0854670 AT2G37260.3 55.882 68 30 0 160 227 86 153 3.39e-21 91.7
Msa0854670 AT2G37260.2 55.882 68 30 0 160 227 84 151 3.41e-21 91.7
Msa0854670 AT2G37260.1 55.882 68 30 0 160 227 166 233 3.55e-21 92.4
Msa0854670 AT1G13960.1 56.061 66 29 0 159 224 229 294 5.85e-21 92.4
Msa0854670 AT1G13960.1 50.877 57 27 1 159 214 409 465 2.81e-13 69.7
Msa0854670 AT1G13960.2 56.061 66 29 0 159 224 76 141 1.41e-20 90.1
Msa0854670 AT1G13960.2 50.877 57 27 1 159 214 256 312 1.10e-12 67.4
Msa0854670 AT4G26640.7 44.737 114 48 3 140 239 142 254 2.44e-20 90.5
Msa0854670 AT4G26640.7 44.186 86 45 2 159 243 341 424 8.72e-14 71.2
Msa0854670 AT4G26640.2 44.737 114 48 3 140 239 182 294 2.48e-20 90.5
Msa0854670 AT4G26640.2 44.186 86 45 2 159 243 381 464 8.64e-14 71.2
Msa0854670 AT4G26640.5 44.737 114 48 3 140 239 6 118 2.90e-20 89.4
Msa0854670 AT4G26640.5 44.186 86 45 2 159 243 205 288 1.01e-13 70.9
Msa0854670 AT4G26640.6 44.737 114 48 3 140 239 6 118 2.90e-20 89.4
Msa0854670 AT4G26640.6 44.186 86 45 2 159 243 205 288 1.01e-13 70.9
Msa0854670 AT4G26640.4 44.737 114 48 3 140 239 6 118 2.90e-20 89.4
Msa0854670 AT4G26640.4 44.186 86 45 2 159 243 205 288 1.01e-13 70.9
Msa0854670 AT4G26640.3 44.737 114 48 3 140 239 6 118 2.90e-20 89.4
Msa0854670 AT4G26640.3 44.186 86 45 2 159 243 205 288 1.01e-13 70.9
Msa0854670 AT4G26640.1 44.737 114 48 3 140 239 110 222 2.96e-20 90.1
Msa0854670 AT4G26640.1 44.186 86 45 2 159 243 309 392 9.31e-14 71.2
Msa0854670 AT2G04880.1 48.649 74 38 0 144 217 96 169 8.30e-20 89.0
Msa0854670 AT2G04880.1 47.458 59 30 1 160 217 308 366 5.31e-11 62.8
Msa0854670 AT2G04880.2 48.649 74 38 0 144 217 96 169 9.86e-20 88.6
Msa0854670 AT2G04880.2 47.458 59 30 1 160 217 284 342 5.72e-11 62.8
Msa0854670 AT2G03340.1 54.412 68 31 0 159 226 250 317 1.99e-19 87.8
Msa0854670 AT2G03340.1 48.387 62 31 1 159 219 415 476 1.78e-13 70.5
Msa0854670 AT4G26440.1 61.017 59 23 0 159 217 178 236 4.96e-19 86.7
Msa0854670 AT4G26440.1 47.826 69 32 2 159 226 372 437 9.08e-12 65.1
Msa0854670 AT3G01080.3 57.627 59 25 0 159 217 205 263 1.83e-18 84.0
Msa0854670 AT3G01080.2 57.627 59 25 0 159 217 205 263 1.04e-17 82.8
Msa0854670 AT3G01080.2 51.667 60 28 1 159 217 344 403 4.79e-15 74.7
Msa0854670 AT3G01080.1 57.627 59 25 0 159 217 167 225 1.53e-17 82.0
Msa0854670 AT3G01080.1 51.667 60 28 1 159 217 306 365 7.37e-15 74.3
Msa0854670 AT4G12020.10 44.706 85 43 2 146 230 457 537 3.06e-16 79.0
Msa0854670 AT4G12020.6 44.706 85 43 2 146 230 457 537 3.19e-16 79.0
Msa0854670 AT4G12020.8 44.706 85 43 2 146 230 457 537 3.19e-16 79.0
Msa0854670 AT4G12020.3 44.706 85 43 2 146 230 457 537 3.24e-16 79.0
Msa0854670 AT4G12020.5 44.706 85 43 2 146 230 390 470 3.58e-16 78.6
Msa0854670 AT4G12020.7 44.706 85 43 2 146 230 457 537 3.60e-16 78.6
Msa0854670 AT4G12020.4 44.706 85 43 2 146 230 457 537 3.69e-16 78.6
Msa0854670 AT4G12020.2 44.706 85 43 2 146 230 457 537 3.69e-16 78.6
Msa0854670 AT4G12020.1 44.706 85 43 2 146 230 457 537 3.78e-16 78.6
Msa0854670 AT4G12020.9 44.706 85 43 2 146 230 457 537 3.78e-16 78.6
Msa0854670 AT4G12020.11 44.706 85 43 2 146 230 457 537 6.02e-16 78.2
Msa0854670 AT2G30250.1 39.474 114 49 3 157 250 164 277 1.27e-15 76.3
Msa0854670 AT2G30250.1 50.820 61 29 1 160 219 329 389 3.25e-13 69.3
Msa0854670 AT1G55600.1 42.500 80 40 2 146 219 289 368 1.18e-13 70.9
Msa0854670 AT2G25000.2 32.653 98 60 3 128 223 76 169 1.52e-13 68.6
Msa0854670 AT2G25000.4 32.653 98 60 3 128 223 76 169 1.52e-13 68.6
Msa0854670 AT2G25000.3 32.653 98 60 3 128 223 98 191 2.47e-13 68.6
Msa0854670 AT2G25000.1 32.653 98 60 3 128 223 119 212 4.17e-13 68.2
Msa0854670 AT1G29860.1 45.205 73 38 2 159 230 136 207 6.51e-13 67.8
Msa0854670 AT5G46350.1 49.180 61 30 1 159 218 183 243 1.37e-12 67.0
Msa0854670 AT4G18170.1 48.387 62 31 1 159 219 172 233 1.65e-12 66.6
Msa0854670 AT5G13080.1 45.614 57 30 1 159 214 67 123 2.57e-12 63.5
Msa0854670 AT2G44745.1 29.861 144 82 3 90 214 58 201 2.72e-12 65.1
Msa0854670 AT2G47260.1 46.032 63 33 1 159 220 174 236 6.17e-12 65.1
Msa0854670 AT1G64000.1 43.860 57 31 1 159 214 114 170 3.62e-11 61.2
Msa0854670 AT2G21900.1 45.902 61 32 1 159 218 109 169 3.94e-11 61.2
Msa0854670 AT5G49520.1 46.774 62 32 1 157 217 219 280 5.06e-11 62.8
Msa0854670 AT2G46130.1 47.368 57 29 1 159 214 30 86 8.11e-11 58.5

Find 33 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATTCAAGGCATGAAAT+TGG 0.216559 5_4:+47397658 Msa0854670:CDS
ATTCTTGCTGAGAGCTCATT+TGG 0.246775 5_4:+47397369 Msa0854670:CDS
ATCATGAGGATGGGTATAAT+TGG 0.303410 5_4:+47397803 Msa0854670:CDS
GATCTCCTCAATTTCTTCCT+TGG 0.315896 5_4:+47397544 Msa0854670:CDS
ATCATTTGGAAGTGAACAAT+TGG 0.346812 5_4:+47398071 Msa0854670:CDS
ATTGATACTGAAGACTTTGC+AGG 0.359791 5_4:-47398108 None:intergenic
TTTGTTCGAAATTGAATTCA+AGG 0.362398 5_4:+47397647 Msa0854670:CDS
GACTTGCAAGATCAATCATT+TGG 0.392633 5_4:+47398057 Msa0854670:CDS
TTCTTGCTGAGAGCTCATTT+GGG 0.400134 5_4:+47397370 Msa0854670:CDS
TAATTGTTTAGTGAAGAAAA+AGG 0.403951 5_4:+47397894 Msa0854670:CDS
TTATCAAGACAGATTGATCT+TGG 0.404285 5_4:+47397511 Msa0854670:CDS
TGATTGATCTTGCAAGTCAA+TGG 0.431766 5_4:-47398052 None:intergenic
TATGAAGATAAAGTAGCAAA+AGG 0.433124 5_4:+47397832 Msa0854670:CDS
GATTGATCTTGCAAGTCAAT+GGG 0.437794 5_4:-47398051 None:intergenic
AAGGTTGAGAGAACAATAGA+TGG 0.440547 5_4:+47397913 Msa0854670:CDS
TTTCAATCTGATTATAGTAA+TGG 0.460671 5_4:+47397745 Msa0854670:CDS
CACAAGAAATCATACTCAAT+AGG 0.466199 5_4:-47397462 None:intergenic
AAAATCAGTCTTTAATAAGA+AGG 0.492421 5_4:+47397776 Msa0854670:CDS
AGAAGGATGAATCATGAGGA+TGG 0.497918 5_4:+47397793 Msa0854670:CDS
GAAATCATACTCAATAGGTC+TGG 0.504110 5_4:-47397457 None:intergenic
AGGATGGGTATAATTGGAAA+AGG 0.511005 5_4:+47397809 Msa0854670:CDS
AAATTCCAAGGAAGAAATTG+AGG 0.514721 5_4:-47397549 None:intergenic
GATGTAGCTATAGAAATTGA+TGG 0.517372 5_4:+47397700 Msa0854670:CDS
ATTGATCTTCATCAATTGAA+CGG 0.533833 5_4:-47397575 None:intergenic
ATTGAACGGTTAAAATTCCA+AGG 0.544574 5_4:-47397561 None:intergenic
AGCCAACGTCTTCAATGAAG+AGG 0.561007 5_4:+47397980 Msa0854670:CDS
TTCCTCTTCATTGAAGACGT+TGG 0.567475 5_4:-47397982 None:intergenic
GAAGGATGAATCATGAGGAT+GGG 0.583422 5_4:+47397794 Msa0854670:CDS
TCAATGGGGCCAGTCTCTGA+AGG 0.613705 5_4:-47398036 None:intergenic
AATAAGAAGGATGAATCATG+AGG 0.620841 5_4:+47397789 Msa0854670:CDS
ATTGATCTTGCAAGTCAATG+GGG 0.684106 5_4:-47398050 None:intergenic
AAGTGAATAAAGATACTCAG+AGG 0.703321 5_4:-47398010 None:intergenic
GAAGCTATTATAAATGTACG+TGG 0.742371 5_4:+47397869 Msa0854670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCAATCTGATTATAGTAA+TGG + chr5_4:47397745-47397764 Msa0854670:CDS 20.0%
!!! TTATTAAAGACTGATTTTGT+TGG - chr5_4:47397774-47397793 None:intergenic 20.0%
!! AAAATCAGTCTTTAATAAGA+AGG + chr5_4:47397776-47397795 Msa0854670:CDS 20.0%
!!! TTTTTCTTCACTAAACAATT+AGG - chr5_4:47397895-47397914 None:intergenic 20.0%
!! TAATTGTTTAGTGAAGAAAA+AGG + chr5_4:47397894-47397913 Msa0854670:CDS 20.0%
!! TTTCAATCTGATTATAGTAA+TGG + chr5_4:47397745-47397764 Msa0854670:CDS 20.0%
!!! TTATTAAAGACTGATTTTGT+TGG - chr5_4:47397774-47397793 None:intergenic 20.0%
!! AAAATCAGTCTTTAATAAGA+AGG + chr5_4:47397776-47397795 Msa0854670:CDS 20.0%
!!! TTTTTCTTCACTAAACAATT+AGG - chr5_4:47397895-47397914 None:intergenic 20.0%
!! TAATTGTTTAGTGAAGAAAA+AGG + chr5_4:47397894-47397913 Msa0854670:CDS 20.0%
! TGAAAAAATGGAAACTTTCA+AGG + chr5_4:47397344-47397363 Msa0854670:CDS 25.0%
! ATTGATCTTCATCAATTGAA+CGG - chr5_4:47397578-47397597 None:intergenic 25.0%
! TTTGTTCGAAATTGAATTCA+AGG + chr5_4:47397647-47397666 Msa0854670:CDS 25.0%
! TATGAAGATAAAGTAGCAAA+AGG + chr5_4:47397832-47397851 Msa0854670:CDS 25.0%
! TGAAAAAATGGAAACTTTCA+AGG + chr5_4:47397344-47397363 Msa0854670:CDS 25.0%
! ATTGATCTTCATCAATTGAA+CGG - chr5_4:47397578-47397597 None:intergenic 25.0%
! TTTGTTCGAAATTGAATTCA+AGG + chr5_4:47397647-47397666 Msa0854670:CDS 25.0%
! TATGAAGATAAAGTAGCAAA+AGG + chr5_4:47397832-47397851 Msa0854670:CDS 25.0%
!! TTTTTCGTTCGAAGCTTTAT+CGG - chr5_4:47397437-47397456 None:intergenic 30.0%
CACAAGAAATCATACTCAAT+AGG - chr5_4:47397465-47397484 None:intergenic 30.0%
GCAAAAAGACATTAAAGCAA+AGG - chr5_4:47397491-47397510 None:intergenic 30.0%
TTATCAAGACAGATTGATCT+TGG + chr5_4:47397511-47397530 Msa0854670:CDS 30.0%
AAATTCCAAGGAAGAAATTG+AGG - chr5_4:47397552-47397571 None:intergenic 30.0%
ATTGAACGGTTAAAATTCCA+AGG - chr5_4:47397564-47397583 None:intergenic 30.0%
TTGAATTCAAGGCATGAAAT+TGG + chr5_4:47397658-47397677 Msa0854670:CDS 30.0%
GATGTAGCTATAGAAATTGA+TGG + chr5_4:47397700-47397719 Msa0854670:CDS 30.0%
AATAAGAAGGATGAATCATG+AGG + chr5_4:47397789-47397808 Msa0854670:CDS 30.0%
GAAGCTATTATAAATGTACG+TGG + chr5_4:47397869-47397888 Msa0854670:CDS 30.0%
AAGTGAATAAAGATACTCAG+AGG - chr5_4:47398013-47398032 None:intergenic 30.0%
ATCATTTGGAAGTGAACAAT+TGG + chr5_4:47398071-47398090 Msa0854670:CDS 30.0%
!! TTTTTCGTTCGAAGCTTTAT+CGG - chr5_4:47397437-47397456 None:intergenic 30.0%
CACAAGAAATCATACTCAAT+AGG - chr5_4:47397465-47397484 None:intergenic 30.0%
GCAAAAAGACATTAAAGCAA+AGG - chr5_4:47397491-47397510 None:intergenic 30.0%
TTATCAAGACAGATTGATCT+TGG + chr5_4:47397511-47397530 Msa0854670:CDS 30.0%
AAATTCCAAGGAAGAAATTG+AGG - chr5_4:47397552-47397571 None:intergenic 30.0%
ATTGAACGGTTAAAATTCCA+AGG - chr5_4:47397564-47397583 None:intergenic 30.0%
TTGAATTCAAGGCATGAAAT+TGG + chr5_4:47397658-47397677 Msa0854670:CDS 30.0%
GATGTAGCTATAGAAATTGA+TGG + chr5_4:47397700-47397719 Msa0854670:CDS 30.0%
AATAAGAAGGATGAATCATG+AGG + chr5_4:47397789-47397808 Msa0854670:CDS 30.0%
GAAGCTATTATAAATGTACG+TGG + chr5_4:47397869-47397888 Msa0854670:CDS 30.0%
AAGTGAATAAAGATACTCAG+AGG - chr5_4:47398013-47398032 None:intergenic 30.0%
ATCATTTGGAAGTGAACAAT+TGG + chr5_4:47398071-47398090 Msa0854670:CDS 30.0%
GAAATCATACTCAATAGGTC+TGG - chr5_4:47397460-47397479 None:intergenic 35.0%
ATCATGAGGATGGGTATAAT+TGG + chr5_4:47397803-47397822 Msa0854670:CDS 35.0%
AGGATGGGTATAATTGGAAA+AGG + chr5_4:47397809-47397828 Msa0854670:CDS 35.0%
AAGGTTGAGAGAACAATAGA+TGG + chr5_4:47397913-47397932 Msa0854670:CDS 35.0%
ATTGATCTTGCAAGTCAATG+GGG - chr5_4:47398053-47398072 None:intergenic 35.0%
GATTGATCTTGCAAGTCAAT+GGG - chr5_4:47398054-47398073 None:intergenic 35.0%
TGATTGATCTTGCAAGTCAA+TGG - chr5_4:47398055-47398074 None:intergenic 35.0%
GACTTGCAAGATCAATCATT+TGG + chr5_4:47398057-47398076 Msa0854670:CDS 35.0%
ATTGATACTGAAGACTTTGC+AGG - chr5_4:47398111-47398130 None:intergenic 35.0%
GAAATCATACTCAATAGGTC+TGG - chr5_4:47397460-47397479 None:intergenic 35.0%
ATCATGAGGATGGGTATAAT+TGG + chr5_4:47397803-47397822 Msa0854670:CDS 35.0%
AGGATGGGTATAATTGGAAA+AGG + chr5_4:47397809-47397828 Msa0854670:CDS 35.0%
AAGGTTGAGAGAACAATAGA+TGG + chr5_4:47397913-47397932 Msa0854670:CDS 35.0%
ATTGATCTTGCAAGTCAATG+GGG - chr5_4:47398053-47398072 None:intergenic 35.0%
GATTGATCTTGCAAGTCAAT+GGG - chr5_4:47398054-47398073 None:intergenic 35.0%
TGATTGATCTTGCAAGTCAA+TGG - chr5_4:47398055-47398074 None:intergenic 35.0%
GACTTGCAAGATCAATCATT+TGG + chr5_4:47398057-47398076 Msa0854670:CDS 35.0%
ATTGATACTGAAGACTTTGC+AGG - chr5_4:47398111-47398130 None:intergenic 35.0%
ATTCTTGCTGAGAGCTCATT+TGG + chr5_4:47397369-47397388 Msa0854670:CDS 40.0%
TTCTTGCTGAGAGCTCATTT+GGG + chr5_4:47397370-47397389 Msa0854670:CDS 40.0%
GATCTCCTCAATTTCTTCCT+TGG + chr5_4:47397544-47397563 Msa0854670:CDS 40.0%
AGAAGGATGAATCATGAGGA+TGG + chr5_4:47397793-47397812 Msa0854670:CDS 40.0%
GAAGGATGAATCATGAGGAT+GGG + chr5_4:47397794-47397813 Msa0854670:CDS 40.0%
TTCCTCTTCATTGAAGACGT+TGG - chr5_4:47397985-47398004 None:intergenic 40.0%
!! TCACTTTTACCTTCAGAGAC+TGG + chr5_4:47398027-47398046 Msa0854670:CDS 40.0%
ATTCTTGCTGAGAGCTCATT+TGG + chr5_4:47397369-47397388 Msa0854670:CDS 40.0%
TTCTTGCTGAGAGCTCATTT+GGG + chr5_4:47397370-47397389 Msa0854670:CDS 40.0%
GATCTCCTCAATTTCTTCCT+TGG + chr5_4:47397544-47397563 Msa0854670:CDS 40.0%
AGAAGGATGAATCATGAGGA+TGG + chr5_4:47397793-47397812 Msa0854670:CDS 40.0%
GAAGGATGAATCATGAGGAT+GGG + chr5_4:47397794-47397813 Msa0854670:CDS 40.0%
TTCCTCTTCATTGAAGACGT+TGG - chr5_4:47397985-47398004 None:intergenic 40.0%
!! TCACTTTTACCTTCAGAGAC+TGG + chr5_4:47398027-47398046 Msa0854670:CDS 40.0%
AGCCAACGTCTTCAATGAAG+AGG + chr5_4:47397980-47397999 Msa0854670:CDS 45.0%
AGCCAACGTCTTCAATGAAG+AGG + chr5_4:47397980-47397999 Msa0854670:CDS 45.0%
TCAATGGGGCCAGTCTCTGA+AGG - chr5_4:47398039-47398058 None:intergenic 55.0%
TCAATGGGGCCAGTCTCTGA+AGG - chr5_4:47398039-47398058 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 47397334 47398134 47397334 ID=Msa0854670;Name=Msa0854670
chr5_4 mRNA 47397334 47398134 47397334 ID=Msa0854670-mRNA-1;Parent=Msa0854670;Name=Msa0854670-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|266
chr5_4 exon 47397334 47398134 47397334 ID=Msa0854670-mRNA-1:exon:14961;Parent=Msa0854670-mRNA-1
chr5_4 CDS 47397334 47398134 47397334 ID=Msa0854670-mRNA-1:cds;Parent=Msa0854670-mRNA-1
Gene Sequence

>Msa0854670

ATGTTATTTTTGAAAAAATGGAAACTTTCAAGGAGATTCTTGCTGAGAGCTCATTTGGGTGTTCTTGTTTTGTTGCTCACATCTTTGTACTCTTTTGTCTCCGATAAAGCTTCGAACGAAAAACCAGACCTATTGAGTATGATTTCTTGTGTTGCCTTTGCTTTAATGTCTTTTTGCTTATCAAGACAGATTGATCTTGGTTTTGAATCTGATCTCCTCAATTTCTTCCTTGGAATTTTAACCGTTCAATTGATGAAGATCAATTTGATTCTGTCTATTGTTGCTGCAATCTTATGTTATTCTCTTATTGTTCTTTGTTCGAAATTGAATTCAAGGCATGAAATTGGAACTTCGAGATTGCAAGATGATGTAGCTATAGAAATTGATGGTGTAGATAACAATAGAAATGAATTTCAATCTGATTATAGTAATGGTAGCCAACAAAATCAGTCTTTAATAAGAAGGATGAATCATGAGGATGGGTATAATTGGAAAAGGTATGAAGATAAAGTAGCAAAAGGAAGTGAAAATCAAAGAAGCTATTATAAATGTACGTGGCCTAATTGTTTAGTGAAGAAAAAGGTTGAGAGAACAATAGATGGAGAAATTATTGAGACACTATACAAAGCTACACATAATCATTGTAAGCCAACGTCTTCAATGAAGAGGAATTCATCCTCTGAGTATCTTTATTCACTTTTACCTTCAGAGACTGGCCCCATTGACTTGCAAGATCAATCATTTGGAAGTGAACAATTGGATTCTGATGAAGAACCTGCAAAGTCTTCAGTATCAATATGA
Protein sequence

>Msa0854670

MLFLKKWKLSRRFLLRAHLGVLVLLLTSLYSFVSDKASNEKPDLLSMISCVAFALMSFCLSRQIDLGFESDLLNFFLGILTVQLMKINLILSIVAAILCYSLIVLCSKLNSRHEIGTSRLQDDVAIEIDGVDNNRNEFQSDYSNGSQQNQSLIRRMNHEDGYNWKRYEDKVAKGSENQRSYYKCTWPNCLVKKKVERTIDGEIIETLYKATHNHCKPTSSMKRNSSSEYLYSLLPSETGPIDLQDQSFGSEQLDSDEEPAKSSVSI*