AlfalfaGEDB | Msa0856430

M. sativa cultivar zhongmu-4 / Msa0856430

KEGG: K09422|K16166
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0856430	XP_003616344.3	90.179	224	16	2	1	223	1	219	1.99e-146	417

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0856430	sp\|Q9FNV9\|MY113_ARATH	60.274	146	52	2	4	143	3	148	1.03e-52	173

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0856430	G7K1V4	90.179	224	16	2	1	223	1	219	3.83e-146	415

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TFs/TRs:

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Gene ID	Type	Classification
Msa0856430	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0856430	MtrunA17_Chr7g0220341	60.833	240	73	4	1	220	17	255	7.02e-97	283
Msa0856430	MtrunA17_Chr5g0434891	88.742	151	11	2	74	223	1	146	1.90e-95	275
Msa0856430	MtrunA17_Chr7g0228991	59.211	228	79	2	7	220	27	254	8.47e-86	255
Msa0856430	MtrunA17_Chr7g0229131	68.478	184	42	3	41	223	4	172	2.30e-83	245
Msa0856430	MtrunA17_Chr5g0434931	61.574	216	81	2	4	218	30	244	2.82e-83	249
Msa0856430	MtrunA17_Chr5g0434911	68.342	199	57	4	1	197	13	207	4.51e-81	243
Msa0856430	MtrunA17_Chr5g0434841	56.332	229	68	4	4	200	15	243	6.87e-77	234
Msa0856430	MtrunA17_Chr8g0360111	57.447	235	67	5	4	220	20	239	2.85e-76	230
Msa0856430	MtrunA17_Chr5g0434831	65.000	180	57	2	4	177	2	181	6.01e-76	227
Msa0856430	MtrunA17_Chr7g0229101	63.743	171	46	3	41	210	4	159	3.74e-59	185

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0856430	AT1G66370.1	60.274	146	52	2	4	143	3	148	1.05e-53	173
Msa0856430	AT1G56650.1	60.839	143	51	2	9	146	8	150	1.67e-46	154
Msa0856430	AT1G66380.1	71.028	107	31	0	5	111	4	110	7.80e-46	149
Msa0856430	AT1G66390.1	58.333	144	54	1	9	146	8	151	1.25e-45	152
Msa0856430	AT5G14750.1	49.650	143	69	2	4	146	11	150	7.93e-44	146
Msa0856430	AT3G13540.1	44.318	176	85	4	9	174	23	195	2.03e-43	146
Msa0856430	AT1G22640.1	58.095	105	44	0	9	113	12	116	5.78e-41	140
Msa0856430	AT5G40330.1	59.804	102	41	0	10	111	13	114	8.03e-41	139
Msa0856430	AT3G27920.1	55.455	110	49	0	6	115	11	120	3.58e-40	137
Msa0856430	AT5G35550.1	54.386	114	52	0	9	122	14	127	1.35e-39	137

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Find 43 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTACGTGGACATCTG+AGG	0.629051	5_4:+51123433	Msa0856430:CDS
AAAGCTTATATTAACAAGTA+TGG	0.371628	5_4:+51123470	Msa0856430:CDS
AAGCTTACCTATTCCCTAGG+AGG	0.610757	5_4:-51124634	None:intergenic
AAGGTTTCTCTGTTGATGGT+TGG	0.484086	5_4:-51124572	None:intergenic
AATTTATTTGAAGCAAATAT+TGG	0.252434	5_4:-51125053	None:intergenic
ACAAGTATGGTGAAGGAAAA+TGG	0.332221	5_4:+51123483	Msa0856430:CDS
ACAATCTCTACCAATTTGAT+CGG	0.538699	5_4:-51125123	None:intergenic
ACATTGCACACATAATATTG+GGG	0.657462	5_4:-51125177	None:intergenic
ACTATGGTTCCCGATCAAAT+TGG	0.451524	5_4:+51125113	Msa0856430:CDS
ACTCATTCACCTTGGTTGAA+TGG	0.394227	5_4:+51125008	Msa0856430:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATTTAATTCCTAAAAGAGC+AGG	+	chr5_4:51123506-51123525	Msa0856430:CDS	30.0%
TGTTTATTCTACTGCGTATT+TGG	+	chr5_4:51123541-51123560	Msa0856430:intron	30.0%
GTTTATTCTACTGCGTATTT+GGG	+	chr5_4:51123542-51123561	Msa0856430:intron	30.0%
TGAAAAGTAATGAAACCACT+AGG	+	chr5_4:51123704-51123723	Msa0856430:intron	30.0%
GAAAAGTAATGAAACCACTA+GGG	+	chr5_4:51123705-51123724	Msa0856430:intron	30.0%
AAAAGTAATGAAACCACTAG+GGG	+	chr5_4:51123706-51123725	Msa0856430:intron	30.0%
TTGAATCTAATTCGATGGTT+TGG	-	chr5_4:51123749-51123768	None:intergenic	30.0%
ACTTTGTCCTTTAACTATCA+TGG	+	chr5_4:51124108-51124127	Msa0856430:intron	30.0%
CCAATACCTAATAGAGTAAA+AGG	-	chr5_4:51124147-51124166	None:intergenic	30.0%
AGATTCGGAACAATAATTAC+AGG	-	chr5_4:51124215-51124234	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	51123404	51125250	51123404	ID=Msa0856430;Name=Msa0856430
chr5_4	mRNA	51123404	51125250	51123404	ID=Msa0856430-mRNA-1;Parent=Msa0856430;Name=Msa0856430-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|3\|0\|223
chr5_4	exon	51123404	51123527	51123404	ID=Msa0856430-mRNA-1:exon:15975;Parent=Msa0856430-mRNA-1
chr5_4	exon	51124519	51124648	51124519	ID=Msa0856430-mRNA-1:exon:15976;Parent=Msa0856430-mRNA-1
chr5_4	exon	51124833	51125250	51124833	ID=Msa0856430-mRNA-1:exon:15977;Parent=Msa0856430-mRNA-1
chr5_4	CDS	51123404	51123527	51123404	ID=Msa0856430-mRNA-1:cds;Parent=Msa0856430-mRNA-1
chr5_4	CDS	51124519	51124648	51124519	ID=Msa0856430-mRNA-1:cds;Parent=Msa0856430-mRNA-1
chr5_4	CDS	51124833	51125250	51124833	ID=Msa0856430-mRNA-1:cds;Parent=Msa0856430-mRNA-1

Gene Sequence

>Msa0856430

ATGTGTAAGGAGAATAACAGAGTCATGAGAAAACGTACGTGGACATCTGAGGAAGACAAGTTACTCAAAGCTTATATTAACAAGTATGGTGAAGGAAAATGGCATTTAATTCCTAAAAGAGCAGGGCTAAATAGGTGCCGAAAAAGTTGCAGATTGAGGTGGTTAAATTATTTAAACCCAACCATCAACAGAGAAACCTTTTCTGAGGATGAAGCTGATATGATCCTTAGGTTACACAACCTCCTAGGGAATAGATGGACGTTGATTGCTGCAAGACTTCAGGGTAGAACAGCTAATGATGTGAAAAACTATTGGCACACCCATTTGAAGAAGGATGTTCGAGGAGCAAAAGAAAAGAAAGAAAAAGATAACCCTAAGGAAATTATTAAAGCTCATGAAGTTATTAAACCTCGTCCAGTAACTTTTTCAACTCATTCACCTTGGTTGAATGGGAAACATAATTTTGTGACACATCCAATATTTGCTTCAAATAAATTTGGTAATGTTCCTAGATATCATGATGACTCATCAGACACTATGGTTCCCGATCAAATTGGTAGAGATTGTGCTTCTAAATCACAACCAAATCTAGGTAGTGCCCCAATATTATGTGTGCAATGTGGTTGCGCTTCATTACAAGAGCAGAACTATAAGGTTTTCAAATGGTTATGA

Protein sequence

>Msa0856430

MCKENNRVMRKRTWTSEEDKLLKAYINKYGEGKWHLIPKRAGLNRCRKSCRLRWLNYLNPTINRETFSEDEADMILRLHNLLGNRWTLIAARLQGRTANDVKNYWHTHLKKDVRGAKEKKEKDNPKEIIKAHEVIKPRPVTFSTHSPWLNGKHNFVTHPIFASNKFGNVPRYHDDSSDTMVPDQIGRDCASKSQPNLGSAPILCVQCGCASLQEQNYKVFKWL*