AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0858530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0858530 MtrunA17_Chr5g0447711 81.350 311 26 3 1 279 1 311 0.0 501
Msa0858530 MtrunA17_Chr5g0447701 48.734 316 108 8 1 262 1 316 2.64e-86 263
Msa0858530 MtrunA17_Chr4g0009881 63.462 104 22 1 63 150 1 104 4.74e-42 140
Msa0858530 MtrunA17_Chr7g0243891 30.769 221 126 4 79 272 233 453 2.71e-27 110
Msa0858530 MtrunA17_Chr4g0004911 34.615 208 106 5 78 262 200 400 5.82e-27 109
Msa0858530 MtrunA17_Chr5g0445311 31.797 217 116 3 79 265 217 431 1.56e-26 108
Msa0858530 MtrunA17_Chr5g0445311 36.000 100 61 2 159 257 161 258 3.71e-11 63.2
Msa0858530 MtrunA17_Chr3g0101131 31.390 223 117 2 79 265 260 482 1.23e-25 105
Msa0858530 MtrunA17_Chr3g0101131 43.284 67 35 2 192 257 237 301 2.03e-11 63.9
Msa0858530 MtrunA17_Chr5g0418191 29.694 229 120 4 78 269 167 391 1.89e-24 101
Msa0858530 MtrunA17_Chr5g0418191 38.043 92 54 2 188 277 139 229 3.62e-11 63.2
Msa0858530 MtrunA17_Chr5g0432161 30.303 231 118 3 79 267 261 490 3.34e-24 101
Msa0858530 MtrunA17_Chr3g0106491 30.531 226 114 6 79 267 215 434 5.18e-23 98.2
Msa0858530 MtrunA17_Chr4g0042831 30.622 209 124 5 79 278 162 358 2.08e-22 95.5
Msa0858530 MtrunA17_Chr4g0042831 36.842 76 48 0 198 273 145 220 1.46e-11 64.3
Msa0858530 MtrunA17_Chr3g0079691 45.122 82 45 0 183 264 480 561 8.87e-19 86.3
Msa0858530 MtrunA17_Chr7g0249301 48.000 75 39 0 186 260 280 354 1.37e-18 84.7
Msa0858530 MtrunA17_Chr7g0249311 43.333 90 51 0 183 272 377 466 1.60e-18 85.5
Msa0858530 MtrunA17_Chr7g0218081 40.230 87 52 0 183 269 395 481 8.44e-18 83.2
Msa0858530 MtrunA17_Chr7g0218081 44.615 65 33 2 194 257 224 286 6.59e-11 62.4
Msa0858530 MtrunA17_Chr7g0263531 44.737 76 42 0 183 258 346 421 3.43e-16 78.2
Msa0858530 MtrunA17_Chr7g0263531 45.588 68 36 1 190 257 181 247 1.02e-11 64.7
Msa0858530 MtrunA17_Chr1g0150801 46.377 69 37 0 186 254 90 158 9.80e-15 70.5
Msa0858530 MtrunA17_Chr8g0383251 42.466 73 42 0 186 258 96 168 1.71e-14 70.1
Msa0858530 MtrunA17_Chr4g0074001 44.000 75 42 0 186 260 347 421 3.11e-14 72.4
Msa0858530 MtrunA17_Chr8g0386241 42.647 68 39 0 187 254 92 159 7.71e-14 68.2
Msa0858530 MtrunA17_Chr7g0270191 43.421 76 43 0 183 258 130 205 3.74e-13 68.2
Msa0858530 MtrunA17_Chr1g0188941 35.211 71 46 0 187 257 40 110 5.11e-13 64.3
Msa0858530 MtrunA17_Chr2g0294231 42.667 75 43 0 183 257 188 262 7.80e-13 67.8
Msa0858530 MtrunA17_Chr4g0051881 45.833 72 39 0 186 257 166 237 7.81e-13 67.8
Msa0858530 MtrunA17_Chr8g0335811 41.892 74 43 0 183 256 139 212 1.03e-12 66.2
Msa0858530 MtrunA17_Chr3g0129321 41.096 73 43 0 186 258 106 178 1.06e-12 65.5
Msa0858530 MtrunA17_Chr7g0224801 42.647 68 39 0 187 254 82 149 2.45e-12 63.9
Msa0858530 MtrunA17_Chr1g0194481 42.667 75 43 0 183 257 143 217 3.52e-12 65.9
Msa0858530 MtrunA17_Chr5g0418781 46.377 69 37 0 186 254 112 180 4.23e-12 65.1
Msa0858530 MtrunA17_Chr2g0333851 35.714 84 53 1 175 257 146 229 7.26e-12 64.7
Msa0858530 MtrunA17_Chr3g0136961 43.056 72 38 2 186 254 124 195 1.11e-11 63.9
Msa0858530 MtrunA17_Chr4g0001361 38.636 88 45 3 189 267 153 240 1.20e-11 63.9
Msa0858530 MtrunA17_Chr5g0443131 43.056 72 41 0 186 257 145 216 1.48e-11 63.5
Msa0858530 MtrunA17_Chr3g0129361 40.541 74 44 0 183 256 129 202 2.17e-11 62.4
Msa0858530 MtrunA17_Chr1g0202461 35.106 94 55 2 192 279 255 348 5.00e-11 62.4
Msa0858530 MtrunA17_Chr4g0068541 38.356 73 45 0 186 258 196 268 7.13e-11 62.0
Msa0858530 MtrunA17_Chr1g0150281 45.070 71 37 2 186 254 127 197 9.93e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0858530 AT2G04880.2 28.660 321 183 6 1 278 46 363 1.05e-32 125
Msa0858530 AT2G04880.1 27.246 345 181 8 1 278 46 387 7.00e-31 120
Msa0858530 AT2G30250.1 31.401 207 116 2 77 257 181 387 6.93e-26 105
Msa0858530 AT3G01080.2 33.511 188 110 3 79 257 222 403 5.00e-25 103
Msa0858530 AT3G01080.1 33.511 188 110 3 79 257 184 365 5.01e-25 103
Msa0858530 AT5G07100.2 32.796 186 100 4 78 260 40 203 4.55e-23 94.7
Msa0858530 AT5G07100.2 45.455 66 36 0 192 257 18 83 8.52e-13 66.6
Msa0858530 AT5G07100.4 32.796 186 100 4 78 260 40 203 4.55e-23 94.7
Msa0858530 AT5G07100.4 45.455 66 36 0 192 257 18 83 8.52e-13 66.6
Msa0858530 AT5G07100.5 32.796 186 100 4 78 260 40 203 4.55e-23 94.7
Msa0858530 AT5G07100.5 45.455 66 36 0 192 257 18 83 8.52e-13 66.6
Msa0858530 AT5G07100.3 32.796 186 100 4 78 260 40 203 4.55e-23 94.7
Msa0858530 AT5G07100.3 45.455 66 36 0 192 257 18 83 8.52e-13 66.6
Msa0858530 AT5G07100.1 32.796 186 100 4 78 260 133 296 1.74e-22 95.1
Msa0858530 AT5G07100.1 45.455 66 36 0 192 257 111 176 1.77e-12 67.0
Msa0858530 AT5G56270.1 47.436 78 41 0 183 260 472 549 3.44e-19 87.8
Msa0858530 AT2G03340.1 41.573 89 52 0 183 271 400 488 5.12e-18 84.0
Msa0858530 AT2G03340.1 42.857 84 47 1 189 272 241 323 1.25e-14 73.9
Msa0858530 AT1G13960.1 45.333 75 41 0 183 257 394 468 7.98e-18 83.6
Msa0858530 AT1G13960.1 46.575 73 38 1 185 257 216 287 1.87e-14 73.6
Msa0858530 AT1G13960.2 45.333 75 41 0 183 257 241 315 8.01e-18 82.8
Msa0858530 AT1G13960.2 46.575 73 38 1 185 257 63 134 2.52e-14 72.8
Msa0858530 AT2G38470.1 45.570 79 43 0 183 261 347 425 2.06e-17 82.4
Msa0858530 AT4G30935.1 30.144 209 109 7 82 258 188 391 8.09e-17 80.5
Msa0858530 AT4G26440.1 39.604 101 58 1 164 264 341 438 1.35e-16 80.1
Msa0858530 AT2G37260.3 26.957 230 110 5 78 254 101 325 2.94e-16 78.2
Msa0858530 AT2G37260.2 26.957 230 110 5 78 254 99 323 2.99e-16 78.2
Msa0858530 AT4G26640.5 44.872 78 43 0 183 260 190 267 3.88e-16 78.2
Msa0858530 AT4G26640.6 44.872 78 43 0 183 260 190 267 3.88e-16 78.2
Msa0858530 AT4G26640.4 44.872 78 43 0 183 260 190 267 3.88e-16 78.2
Msa0858530 AT4G26640.3 44.872 78 43 0 183 260 190 267 3.88e-16 78.2
Msa0858530 AT4G26640.7 44.872 78 43 0 183 260 326 403 4.29e-16 78.2
Msa0858530 AT4G26640.2 44.872 78 43 0 183 260 366 443 4.33e-16 78.6
Msa0858530 AT4G26640.1 44.872 78 43 0 183 260 294 371 4.70e-16 78.2
Msa0858530 AT2G37260.1 26.522 230 111 5 78 254 181 405 1.28e-15 76.6
Msa0858530 AT5G64810.1 42.254 71 41 0 186 256 98 168 2.46e-14 70.5
Msa0858530 AT5G26170.1 44.928 69 38 0 186 254 101 169 3.96e-14 69.3
Msa0858530 AT5G13080.1 44.118 68 38 0 187 254 56 123 6.40e-14 68.2
Msa0858530 AT1G55600.1 45.833 72 39 0 186 257 295 366 1.08e-13 71.2
Msa0858530 AT2G21900.1 40.789 76 43 1 184 257 93 168 3.01e-13 67.4
Msa0858530 AT5G49520.1 43.421 76 43 0 183 258 206 281 6.22e-13 68.6
Msa0858530 AT2G47260.1 42.105 76 44 0 183 258 159 234 6.31e-13 68.2
Msa0858530 AT1G69310.4 44.286 70 39 0 186 255 135 204 2.93e-12 65.9
Msa0858530 AT1G69310.2 44.286 70 39 0 186 255 135 204 2.93e-12 65.9
Msa0858530 AT1G69310.1 44.286 70 39 0 186 255 135 204 2.93e-12 65.9
Msa0858530 AT2G44745.1 41.892 74 43 0 183 256 130 203 3.09e-12 65.1
Msa0858530 AT3G62340.1 46.774 62 33 0 197 258 117 178 5.07e-12 65.1
Msa0858530 AT4G12020.5 41.096 73 42 1 185 257 388 459 7.02e-12 66.2
Msa0858530 AT3G01970.1 42.647 68 39 0 187 254 54 121 7.48e-12 62.4
Msa0858530 AT4G12020.10 41.096 73 42 1 185 257 455 526 8.08e-12 65.9
Msa0858530 AT4G12020.3 41.096 73 42 1 185 257 455 526 8.08e-12 65.9
Msa0858530 AT4G12020.4 41.096 73 42 1 185 257 455 526 8.16e-12 65.9
Msa0858530 AT4G12020.2 41.096 73 42 1 185 257 455 526 8.17e-12 65.9
Msa0858530 AT5G41570.1 42.029 69 40 0 186 254 86 154 1.02e-11 62.8
Msa0858530 AT1G64000.1 42.029 69 40 0 186 254 102 170 1.33e-11 62.8
Msa0858530 AT2G25000.2 35.632 87 52 2 185 267 90 176 1.68e-11 63.2
Msa0858530 AT2G25000.4 35.632 87 52 2 185 267 90 176 1.68e-11 63.2
Msa0858530 AT1G29860.1 41.333 75 44 0 183 257 121 195 1.71e-11 63.5
Msa0858530 AT4G18170.1 44.000 75 42 0 183 257 157 231 2.07e-11 63.9
Msa0858530 AT4G39410.1 41.892 74 43 0 183 256 208 281 3.59e-11 62.8
Msa0858530 AT5G46350.1 46.875 64 34 0 197 260 182 245 5.73e-11 62.4
Msa0858530 AT2G46130.1 39.130 69 42 0 186 254 18 86 6.43e-11 58.9
Msa0858530 AT4G12020.6 41.096 73 42 1 185 257 455 526 9.05e-11 62.8
Msa0858530 AT4G12020.8 41.096 73 42 1 185 257 455 526 9.05e-11 62.8
Msa0858530 AT4G12020.7 41.096 73 42 1 185 257 455 526 9.15e-11 62.8
Msa0858530 AT4G23550.1 37.333 75 46 1 187 260 129 203 9.27e-11 61.6
Msa0858530 AT4G12020.1 41.096 73 42 1 185 257 455 526 9.49e-11 62.8
Msa0858530 AT4G12020.9 41.096 73 42 1 185 257 455 526 9.49e-11 62.8

Find 50 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAATAATGCGTATTAA+TGG 0.253581 5_4:+55987186 Msa0858530:CDS
TAATGTAACAACTACCTATA+AGG 0.261776 5_4:+55987104 Msa0858530:CDS
ATGATCATGTTGTCCCTTAT+AGG 0.293854 5_4:-55987118 None:intergenic
ACTTCCACACCGGTCAAATA+TGG 0.326583 5_4:-55986617 None:intergenic
AATTCATGTGAAGGAGTATT+AGG 0.352642 5_4:-55986299 None:intergenic
TGTTCTTCCTGTTGTTGAAC+AGG 0.360275 5_4:+55986583 Msa0858530:CDS
ACAATGCTTGATTGAGGATT+AGG 0.367320 5_4:-55986545 None:intergenic
GTTACATTAGTCACATTTAG+AGG 0.369899 5_4:-55987090 None:intergenic
ACAATGTACAATATATTAAG+AGG 0.384416 5_4:-55986694 None:intergenic
ACTAGAGTTGGCGTGTTAAC+TGG 0.387351 5_4:+55986745 Msa0858530:CDS
TGAATGTGTGCATTTGTAAT+AGG 0.403229 5_4:-55986441 None:intergenic
AAATTTCATTGGTCAAATGA+TGG 0.419177 5_4:+55986482 Msa0858530:CDS
GTGTATCGCTGGCGCAAATA+CGG 0.423416 5_4:+55986820 Msa0858530:CDS
TCGCTCAATCATACGTGAAA+TGG 0.436400 5_4:+55986349 Msa0858530:CDS
TACTTTCAATGCGCCTATCC+AGG 0.437442 5_4:+55987036 Msa0858530:CDS
AATGTAACAACTACCTATAA+GGG 0.449073 5_4:+55987105 Msa0858530:CDS
ATTAGGATGATTATGCTGAT+TGG 0.483420 5_4:-55986528 None:intergenic
TGTGGTCCCTGTTCAACAAC+AGG 0.490907 5_4:-55986590 None:intergenic
TTTCACTGTGCATCCTGGAT+AGG 0.494274 5_4:-55987049 None:intergenic
TTGACCGGTGTGGAAGTTCA+AGG 0.496310 5_4:+55986623 Msa0858530:CDS
AATTTCATTGGTCAAATGAT+GGG 0.502281 5_4:+55986483 Msa0858530:CDS
GTTCTTCCTGTTGTTGAACA+GGG 0.504635 5_4:+55986584 Msa0858530:CDS
AGAAACTAATGATGTAGCTA+TGG 0.532105 5_4:+55986235 Msa0858530:CDS
TTACATTAGTCACATTTAGA+GGG 0.537229 5_4:-55987089 None:intergenic
AATACTGATGCTACTAGAGT+TGG 0.539414 5_4:+55986733 Msa0858530:CDS
TGACGGACTCCCCTACCAAT+CGG 0.541659 5_4:-55987147 None:intergenic
CGTGGTACAATGCTTGATTG+AGG 0.547291 5_4:-55986551 None:intergenic
TCATGAACCACCGATTGGTA+GGG 0.556990 5_4:+55987137 Msa0858530:CDS
CCACATCAACCATATTTGAC+CGG 0.557825 5_4:+55986608 Msa0858530:CDS
ATCATGAACCACCGATTGGT+AGG 0.562327 5_4:+55987136 Msa0858530:CDS
CATGAACCACCGATTGGTAG+GGG 0.566020 5_4:+55987138 Msa0858530:CDS
TGTAGCTATGGCTTGTGACT+TGG 0.571450 5_4:+55986247 Msa0858530:CDS
TATTGATTAGATTGTATCAG+AGG 0.571636 5_4:-55986323 None:intergenic
CATGATCATGAACCACCGAT+TGG 0.591424 5_4:+55987132 Msa0858530:CDS
ATCAGAGGTAATTCATGTGA+AGG 0.594434 5_4:-55986308 None:intergenic
TTGTAAATGATGTGTATCGC+TGG 0.598613 5_4:+55986809 Msa0858530:CDS
CCGGTCAAATATGGTTGATG+TGG 0.605641 5_4:-55986608 None:intergenic
ACTCGATGTCGTACTTCAGA+AGG 0.611376 5_4:-55986182 None:intergenic
TACATTAGTCACATTTAGAG+GGG 0.623510 5_4:-55987088 None:intergenic
GACAATGTGATCTCCCTCTG+AGG 0.634875 5_4:-55986209 None:intergenic
CACATATGCAACACTCGCAG+AGG 0.656505 5_4:+55986276 Msa0858530:CDS
TATTTGTAACAGAGTCATGA+CGG 0.659620 5_4:-55987164 None:intergenic
GGTGTAACTGAAATATTCGA+CGG 0.662056 5_4:-55986507 None:intergenic
ATCACCTTGAACTTCCACAC+CGG 0.673408 5_4:-55986627 None:intergenic
ACATCGAGTGAATCCTCAGA+GGG 0.680083 5_4:+55986196 Msa0858530:CDS
CACTTGAAGTCTGAACAACA+AGG 0.680480 5_4:-55986777 None:intergenic
TCAACCATATTTGACCGGTG+TGG 0.682556 5_4:+55986613 Msa0858530:CDS
CGGACTCCCCTACCAATCGG+TGG 0.703895 5_4:-55987144 None:intergenic
GGAAGAACATGATCAACTCG+TGG 0.704995 5_4:-55986569 None:intergenic
GACATCGAGTGAATCCTCAG+AGG 0.735585 5_4:+55986195 Msa0858530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGTACAATATATTAAG+AGG - chr5_4:55986697-55986716 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG + chr5_4:55986854-55986873 Msa0858530:CDS 20.0%
!! ACAATGTACAATATATTAAG+AGG - chr5_4:55986697-55986716 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG + chr5_4:55986854-55986873 Msa0858530:CDS 20.0%
!! TATTGATTAGATTGTATCAG+AGG - chr5_4:55986326-55986345 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG + chr5_4:55986471-55986490 Msa0858530:CDS 25.0%
! AAATTTCATTGGTCAAATGA+TGG + chr5_4:55986482-55986501 Msa0858530:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG + chr5_4:55986483-55986502 Msa0858530:CDS 25.0%
! ATGAGTTCAAAATAAAAGCT+AGG - chr5_4:55986885-55986904 None:intergenic 25.0%
! TTACATTAGTCACATTTAGA+GGG - chr5_4:55987092-55987111 None:intergenic 25.0%
! TAATGTAACAACTACCTATA+AGG + chr5_4:55987104-55987123 Msa0858530:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG + chr5_4:55987105-55987124 Msa0858530:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG + chr5_4:55987186-55987205 Msa0858530:CDS 25.0%
!! TATTGATTAGATTGTATCAG+AGG - chr5_4:55986326-55986345 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG + chr5_4:55986471-55986490 Msa0858530:CDS 25.0%
! AAATTTCATTGGTCAAATGA+TGG + chr5_4:55986482-55986501 Msa0858530:CDS 25.0%
! AATTTCATTGGTCAAATGAT+GGG + chr5_4:55986483-55986502 Msa0858530:CDS 25.0%
! ATGAGTTCAAAATAAAAGCT+AGG - chr5_4:55986885-55986904 None:intergenic 25.0%
! TTACATTAGTCACATTTAGA+GGG - chr5_4:55987092-55987111 None:intergenic 25.0%
! TAATGTAACAACTACCTATA+AGG + chr5_4:55987104-55987123 Msa0858530:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG + chr5_4:55987105-55987124 Msa0858530:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG + chr5_4:55987186-55987205 Msa0858530:CDS 25.0%
AGAAACTAATGATGTAGCTA+TGG + chr5_4:55986235-55986254 Msa0858530:CDS 30.0%
!! AATTCATGTGAAGGAGTATT+AGG - chr5_4:55986302-55986321 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG + chr5_4:55986372-55986391 Msa0858530:intron 30.0%
GGCTATAAGTGGAAAAAATA+TGG + chr5_4:55986383-55986402 Msa0858530:intron 30.0%
TGAATGTGTGCATTTGTAAT+AGG - chr5_4:55986444-55986463 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG - chr5_4:55986531-55986550 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG + chr5_4:55986838-55986857 Msa0858530:CDS 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG - chr5_4:55987023-55987042 None:intergenic 30.0%
TACATTAGTCACATTTAGAG+GGG - chr5_4:55987091-55987110 None:intergenic 30.0%
GTTACATTAGTCACATTTAG+AGG - chr5_4:55987093-55987112 None:intergenic 30.0%
TATTTGTAACAGAGTCATGA+CGG - chr5_4:55987167-55987186 None:intergenic 30.0%
AGAAACTAATGATGTAGCTA+TGG + chr5_4:55986235-55986254 Msa0858530:CDS 30.0%
!! AATTCATGTGAAGGAGTATT+AGG - chr5_4:55986302-55986321 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG + chr5_4:55986372-55986391 Msa0858530:intron 30.0%
GGCTATAAGTGGAAAAAATA+TGG + chr5_4:55986383-55986402 Msa0858530:intron 30.0%
TGAATGTGTGCATTTGTAAT+AGG - chr5_4:55986444-55986463 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG - chr5_4:55986531-55986550 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG + chr5_4:55986838-55986857 Msa0858530:CDS 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG - chr5_4:55987023-55987042 None:intergenic 30.0%
TACATTAGTCACATTTAGAG+GGG - chr5_4:55987091-55987110 None:intergenic 30.0%
GTTACATTAGTCACATTTAG+AGG - chr5_4:55987093-55987112 None:intergenic 30.0%
TATTTGTAACAGAGTCATGA+CGG - chr5_4:55987167-55987186 None:intergenic 30.0%
ATCAGAGGTAATTCATGTGA+AGG - chr5_4:55986311-55986330 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG + chr5_4:55986362-55986381 Msa0858530:intron 35.0%
GGTGTAACTGAAATATTCGA+CGG - chr5_4:55986510-55986529 None:intergenic 35.0%
ACAATGCTTGATTGAGGATT+AGG - chr5_4:55986548-55986567 None:intergenic 35.0%
AATACTGATGCTACTAGAGT+TGG + chr5_4:55986733-55986752 Msa0858530:CDS 35.0%
TTGTAAATGATGTGTATCGC+TGG + chr5_4:55986809-55986828 Msa0858530:CDS 35.0%
! GTAATTTCTGGAAGATTGGA+GGG - chr5_4:55987019-55987038 None:intergenic 35.0%
! AGTAATTTCTGGAAGATTGG+AGG - chr5_4:55987020-55987039 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG - chr5_4:55987121-55987140 None:intergenic 35.0%
ATCAGAGGTAATTCATGTGA+AGG - chr5_4:55986311-55986330 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG + chr5_4:55986362-55986381 Msa0858530:intron 35.0%
GGTGTAACTGAAATATTCGA+CGG - chr5_4:55986510-55986529 None:intergenic 35.0%
ACAATGCTTGATTGAGGATT+AGG - chr5_4:55986548-55986567 None:intergenic 35.0%
AATACTGATGCTACTAGAGT+TGG + chr5_4:55986733-55986752 Msa0858530:CDS 35.0%
TTGTAAATGATGTGTATCGC+TGG + chr5_4:55986809-55986828 Msa0858530:CDS 35.0%
! GTAATTTCTGGAAGATTGGA+GGG - chr5_4:55987019-55987038 None:intergenic 35.0%
! AGTAATTTCTGGAAGATTGG+AGG - chr5_4:55987020-55987039 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG - chr5_4:55987121-55987140 None:intergenic 35.0%
TCGCTCAATCATACGTGAAA+TGG + chr5_4:55986349-55986368 Msa0858530:CDS 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG + chr5_4:55986583-55986602 Msa0858530:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG + chr5_4:55986584-55986603 Msa0858530:CDS 40.0%
CCACATCAACCATATTTGAC+CGG + chr5_4:55986608-55986627 Msa0858530:CDS 40.0%
CACTTGAAGTCTGAACAACA+AGG - chr5_4:55986780-55986799 None:intergenic 40.0%
GCGCAAATACGGAAGAAAAA+TGG + chr5_4:55986831-55986850 Msa0858530:CDS 40.0%
GGCGCATTGAAAGTAATTTC+TGG - chr5_4:55987031-55987050 None:intergenic 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG - chr5_4:55987057-55987076 None:intergenic 40.0%
TCGCTCAATCATACGTGAAA+TGG + chr5_4:55986349-55986368 Msa0858530:CDS 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG + chr5_4:55986583-55986602 Msa0858530:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG + chr5_4:55986584-55986603 Msa0858530:CDS 40.0%
CCACATCAACCATATTTGAC+CGG + chr5_4:55986608-55986627 Msa0858530:CDS 40.0%
CACTTGAAGTCTGAACAACA+AGG - chr5_4:55986780-55986799 None:intergenic 40.0%
GCGCAAATACGGAAGAAAAA+TGG + chr5_4:55986831-55986850 Msa0858530:CDS 40.0%
GGCGCATTGAAAGTAATTTC+TGG - chr5_4:55987031-55987050 None:intergenic 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG - chr5_4:55987057-55987076 None:intergenic 40.0%
ACTCGATGTCGTACTTCAGA+AGG - chr5_4:55986185-55986204 None:intergenic 45.0%
ACATCGAGTGAATCCTCAGA+GGG + chr5_4:55986196-55986215 Msa0858530:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG + chr5_4:55986247-55986266 Msa0858530:CDS 45.0%
CGTGGTACAATGCTTGATTG+AGG - chr5_4:55986554-55986573 None:intergenic 45.0%
GGAAGAACATGATCAACTCG+TGG - chr5_4:55986572-55986591 None:intergenic 45.0%
! CCGGTCAAATATGGTTGATG+TGG - chr5_4:55986611-55986630 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG + chr5_4:55986613-55986632 Msa0858530:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG - chr5_4:55986620-55986639 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG - chr5_4:55986630-55986649 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG + chr5_4:55986745-55986764 Msa0858530:CDS 45.0%
TACTTTCAATGCGCCTATCC+AGG + chr5_4:55987036-55987055 Msa0858530:CDS 45.0%
TTTCACTGTGCATCCTGGAT+AGG - chr5_4:55987052-55987071 None:intergenic 45.0%
CATGATCATGAACCACCGAT+TGG + chr5_4:55987132-55987151 Msa0858530:CDS 45.0%
! ATCATGAACCACCGATTGGT+AGG + chr5_4:55987136-55987155 Msa0858530:CDS 45.0%
! TCATGAACCACCGATTGGTA+GGG + chr5_4:55987137-55987156 Msa0858530:CDS 45.0%
ACTCGATGTCGTACTTCAGA+AGG - chr5_4:55986185-55986204 None:intergenic 45.0%
ACATCGAGTGAATCCTCAGA+GGG + chr5_4:55986196-55986215 Msa0858530:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG + chr5_4:55986247-55986266 Msa0858530:CDS 45.0%
CGTGGTACAATGCTTGATTG+AGG - chr5_4:55986554-55986573 None:intergenic 45.0%
GGAAGAACATGATCAACTCG+TGG - chr5_4:55986572-55986591 None:intergenic 45.0%
! CCGGTCAAATATGGTTGATG+TGG - chr5_4:55986611-55986630 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG + chr5_4:55986613-55986632 Msa0858530:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG - chr5_4:55986620-55986639 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG - chr5_4:55986630-55986649 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG + chr5_4:55986745-55986764 Msa0858530:CDS 45.0%
TACTTTCAATGCGCCTATCC+AGG + chr5_4:55987036-55987055 Msa0858530:CDS 45.0%
TTTCACTGTGCATCCTGGAT+AGG - chr5_4:55987052-55987071 None:intergenic 45.0%
CATGATCATGAACCACCGAT+TGG + chr5_4:55987132-55987151 Msa0858530:CDS 45.0%
! ATCATGAACCACCGATTGGT+AGG + chr5_4:55987136-55987155 Msa0858530:CDS 45.0%
! TCATGAACCACCGATTGGTA+GGG + chr5_4:55987137-55987156 Msa0858530:CDS 45.0%
GACATCGAGTGAATCCTCAG+AGG + chr5_4:55986195-55986214 Msa0858530:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG - chr5_4:55986212-55986231 None:intergenic 50.0%
CACATATGCAACACTCGCAG+AGG + chr5_4:55986276-55986295 Msa0858530:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG - chr5_4:55986593-55986612 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG + chr5_4:55986623-55986642 Msa0858530:CDS 50.0%
GTGTATCGCTGGCGCAAATA+CGG + chr5_4:55986820-55986839 Msa0858530:CDS 50.0%
! CATGAACCACCGATTGGTAG+GGG + chr5_4:55987138-55987157 Msa0858530:CDS 50.0%
GACATCGAGTGAATCCTCAG+AGG + chr5_4:55986195-55986214 Msa0858530:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG - chr5_4:55986212-55986231 None:intergenic 50.0%
CACATATGCAACACTCGCAG+AGG + chr5_4:55986276-55986295 Msa0858530:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG - chr5_4:55986593-55986612 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG + chr5_4:55986623-55986642 Msa0858530:CDS 50.0%
GTGTATCGCTGGCGCAAATA+CGG + chr5_4:55986820-55986839 Msa0858530:CDS 50.0%
! CATGAACCACCGATTGGTAG+GGG + chr5_4:55987138-55987157 Msa0858530:CDS 50.0%
!! TGACGGACTCCCCTACCAAT+CGG - chr5_4:55987150-55987169 None:intergenic 55.0%
!! TGACGGACTCCCCTACCAAT+CGG - chr5_4:55987150-55987169 None:intergenic 55.0%
CGGACTCCCCTACCAATCGG+TGG - chr5_4:55987147-55987166 None:intergenic 65.0%
CGGACTCCCCTACCAATCGG+TGG - chr5_4:55987147-55987166 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 55986143 55987218 55986143 ID=Msa0858530;Name=Msa0858530
chr5_4 mRNA 55986143 55987218 55986143 ID=Msa0858530-mRNA-1;Parent=Msa0858530;Name=Msa0858530-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0.33|0.25|4|0|279
chr5_4 exon 55986143 55986370 55986143 ID=Msa0858530-mRNA-1:exon:17130;Parent=Msa0858530-mRNA-1
chr5_4 exon 55986422 55986644 55986422 ID=Msa0858530-mRNA-1:exon:17131;Parent=Msa0858530-mRNA-1
chr5_4 exon 55986674 55986875 55986674 ID=Msa0858530-mRNA-1:exon:17132;Parent=Msa0858530-mRNA-1
chr5_4 exon 55987032 55987218 55987032 ID=Msa0858530-mRNA-1:exon:17133;Parent=Msa0858530-mRNA-1
chr5_4 CDS 55986143 55986370 55986143 ID=Msa0858530-mRNA-1:cds;Parent=Msa0858530-mRNA-1
chr5_4 CDS 55986422 55986644 55986422 ID=Msa0858530-mRNA-1:cds;Parent=Msa0858530-mRNA-1
chr5_4 CDS 55986674 55986875 55986674 ID=Msa0858530-mRNA-1:cds;Parent=Msa0858530-mRNA-1
chr5_4 CDS 55987032 55987218 55987032 ID=Msa0858530-mRNA-1:cds;Parent=Msa0858530-mRNA-1
Gene Sequence

>Msa0858530

ATGGTTTTGTCACTACAAAATGTTGATCACAATGATTCTCCTTCTGAAGTACGACATCGAGTGAATCCTCAGAGGGAGATCACATTGTCACGAGAAACTAATGATGTAGCTATGGCTTGTGACTTGGTATTATCACATATGCAACACTCGCAGAGGCCTAATACTCCTTCACATGAATTACCTCTGATACAATCTAATCAATACACTCGCTCAATCATACGTGAAATGAAGAATGAACATAAAAGAGCCTATTACAAATGCACACATTCAGACTGTCGTGCAAAAAAGAAATTTCATTGGTCAAATGATGGGACCGTCGAATATTTCAGTTACACCAATCAGCATAATCATCCTAATCCTCAATCAAGCATTGTACCACGAGTTGATCATGTTCTTCCTGTTGTTGAACAGGGACCACATCAACCATATTTGACCGGTGTGGAAGTTCAAGTTTCAATATTACATGAAAAACCTCTTAATATATTGTACATTGTTGTGCATGCAGACAACAATACTGATGCTACTAGAGTTGGCGTGTTAACTGGTGAACTTCACCTTGTTGTTCAGACTTCAAGTGCAAATGAAGTTGTAAATGATGTGTATCGCTGGCGCAAATACGGAAGAAAAATGGTTAATGGAAAAACAATTCAAAGAAATTACTTTCAATGCGCCTATCCAGGATGCACAGTGAAAAAGTATGTTGAAAAGTCCCCTCTAAATGTGACTAATGTAACAACTACCTATAAGGGACAACATGATCATGAACCACCGATTGGTAGGGGAGTCCGTCATGACTCTGTTACAAATACTCAAATAATGCGTATTAATGGCGAGCCATAA
Protein sequence

>Msa0858530

MVLSLQNVDHNDSPSEVRHRVNPQREITLSRETNDVAMACDLVLSHMQHSQRPNTPSHELPLIQSNQYTRSIIREMKNEHKRAYYKCTHSDCRAKKKFHWSNDGTVEYFSYTNQHNHPNPQSSIVPRVDHVLPVVEQGPHQPYLTGVEVQVSILHEKPLNILYIVVHADNNTDATRVGVLTGELHLVVQTSSANEVVNDVYRWRKYGRKMVNGKTIQRNYFQCAYPGCTVKKYVEKSPLNVTNVTTTYKGQHDHEPPIGRGVRHDSVTNTQIMRINGEP*