AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0858560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0858560 MtrunA17_Chr5g0447711 82.315 311 23 3 1 279 1 311 0.0 508
Msa0858560 MtrunA17_Chr5g0447701 49.367 316 106 8 1 262 1 316 9.20e-89 269
Msa0858560 MtrunA17_Chr4g0009881 63.462 104 22 1 63 150 1 104 6.70e-42 140
Msa0858560 MtrunA17_Chr7g0243891 30.769 221 126 4 79 272 233 453 1.31e-27 111
Msa0858560 MtrunA17_Chr4g0004911 34.135 208 107 5 78 262 200 400 1.34e-27 110
Msa0858560 MtrunA17_Chr5g0445311 30.876 217 118 3 79 265 217 431 1.93e-26 108
Msa0858560 MtrunA17_Chr5g0445311 36.000 100 61 2 159 257 161 258 1.34e-11 64.7
Msa0858560 MtrunA17_Chr3g0101131 31.390 223 117 2 79 265 260 482 6.29e-26 107
Msa0858560 MtrunA17_Chr3g0101131 43.284 67 35 2 192 257 237 301 7.23e-12 65.5
Msa0858560 MtrunA17_Chr5g0432161 30.303 231 118 3 79 267 261 490 1.59e-25 105
Msa0858560 MtrunA17_Chr3g0106491 30.909 220 121 4 79 267 215 434 8.19e-25 103
Msa0858560 MtrunA17_Chr3g0106491 43.548 62 34 1 198 259 198 258 8.04e-11 62.0
Msa0858560 MtrunA17_Chr5g0418191 29.646 226 124 3 78 269 167 391 1.18e-24 102
Msa0858560 MtrunA17_Chr5g0418191 38.043 92 54 2 188 277 139 229 1.02e-11 64.7
Msa0858560 MtrunA17_Chr4g0042831 30.622 209 124 5 79 278 162 358 7.08e-23 96.7
Msa0858560 MtrunA17_Chr4g0042831 36.842 76 48 0 198 273 145 220 3.91e-12 65.9
Msa0858560 MtrunA17_Chr3g0079691 45.122 82 45 0 183 264 480 561 2.87e-19 87.4
Msa0858560 MtrunA17_Chr7g0249311 43.333 90 51 0 183 272 377 466 4.22e-19 87.0
Msa0858560 MtrunA17_Chr7g0249301 48.000 75 39 0 186 260 280 354 4.26e-19 86.3
Msa0858560 MtrunA17_Chr7g0218081 40.230 87 52 0 183 269 395 481 2.48e-18 84.7
Msa0858560 MtrunA17_Chr7g0218081 44.615 65 33 2 194 257 224 286 1.98e-11 63.9
Msa0858560 MtrunA17_Chr7g0263531 46.053 76 41 0 183 258 346 421 3.04e-17 81.3
Msa0858560 MtrunA17_Chr7g0263531 45.588 68 36 1 190 257 181 247 3.53e-12 66.2
Msa0858560 MtrunA17_Chr1g0150801 47.826 69 36 0 186 254 90 158 7.36e-16 73.6
Msa0858560 MtrunA17_Chr4g0074001 45.333 75 41 0 186 260 347 421 2.40e-15 75.9
Msa0858560 MtrunA17_Chr8g0383251 42.466 73 42 0 186 258 96 168 4.38e-15 71.6
Msa0858560 MtrunA17_Chr8g0386241 42.647 68 39 0 187 254 92 159 1.38e-14 70.5
Msa0858560 MtrunA17_Chr7g0270191 44.737 76 42 0 183 258 130 205 3.20e-14 71.2
Msa0858560 MtrunA17_Chr4g0051881 47.222 72 38 0 186 257 166 237 5.97e-14 70.9
Msa0858560 MtrunA17_Chr2g0294231 44.000 75 42 0 183 257 188 262 7.00e-14 70.9
Msa0858560 MtrunA17_Chr8g0335811 43.243 74 42 0 183 256 139 212 8.20e-14 69.3
Msa0858560 MtrunA17_Chr7g0224801 44.118 68 38 0 187 254 82 149 1.20e-13 67.4
Msa0858560 MtrunA17_Chr1g0188941 35.211 71 46 0 187 257 40 110 1.30e-13 65.9
Msa0858560 MtrunA17_Chr3g0129321 41.096 73 43 0 186 258 106 178 2.69e-13 67.4
Msa0858560 MtrunA17_Chr1g0194481 44.000 75 42 0 183 257 143 217 2.75e-13 68.9
Msa0858560 MtrunA17_Chr5g0418781 47.826 69 36 0 186 254 112 180 3.51e-13 68.2
Msa0858560 MtrunA17_Chr5g0443131 44.444 72 40 0 186 257 145 216 1.17e-12 67.0
Msa0858560 MtrunA17_Chr3g0129361 41.892 74 43 0 183 256 129 202 1.70e-12 65.5
Msa0858560 MtrunA17_Chr2g0333851 35.714 84 53 1 175 257 146 229 2.33e-12 66.2
Msa0858560 MtrunA17_Chr3g0136961 43.056 72 38 2 186 254 124 195 2.64e-12 65.9
Msa0858560 MtrunA17_Chr4g0001361 36.957 92 49 3 185 267 149 240 3.21e-12 65.9
Msa0858560 MtrunA17_Chr4g0068541 39.726 73 44 0 186 258 196 268 5.62e-12 65.5
Msa0858560 MtrunA17_Chr1g0150281 46.479 71 36 2 186 254 127 197 7.44e-12 64.3
Msa0858560 MtrunA17_Chr7g0270831 36.709 79 48 2 188 264 206 284 1.01e-11 64.7
Msa0858560 MtrunA17_Chr8g0383351 45.161 62 34 0 195 256 157 218 1.86e-11 62.8
Msa0858560 MtrunA17_Chr7g0248721 38.272 81 49 1 174 254 117 196 2.33e-11 62.0
Msa0858560 MtrunA17_Chr1g0202461 36.170 94 54 2 192 279 255 348 2.59e-11 63.5
Msa0858560 MtrunA17_Chr4g0025061 30.928 97 66 1 164 259 326 422 3.02e-11 63.5
Msa0858560 MtrunA17_Chr3g0099641 32.967 91 60 1 188 277 241 331 3.81e-11 63.2
Msa0858560 MtrunA17_Chr5g0432071 39.130 69 41 1 198 266 104 171 5.30e-11 60.8
Msa0858560 MtrunA17_Chr2g0288871 31.915 94 63 1 165 257 272 365 7.64e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0858560 AT2G04880.2 28.972 321 182 6 1 278 46 363 7.26e-34 128
Msa0858560 AT2G04880.1 27.536 345 180 8 1 278 46 387 1.72e-31 122
Msa0858560 AT2G30250.1 32.367 207 114 3 77 257 181 387 3.99e-26 106
Msa0858560 AT3G01080.1 31.915 188 113 3 79 257 184 365 1.83e-25 104
Msa0858560 AT3G01080.2 32.979 188 111 2 79 257 222 403 3.03e-25 104
Msa0858560 AT5G07100.2 32.796 186 100 4 78 260 40 203 1.13e-23 96.3
Msa0858560 AT5G07100.2 45.455 66 36 0 192 257 18 83 2.32e-13 68.2
Msa0858560 AT5G07100.4 32.796 186 100 4 78 260 40 203 1.13e-23 96.3
Msa0858560 AT5G07100.4 45.455 66 36 0 192 257 18 83 2.32e-13 68.2
Msa0858560 AT5G07100.5 32.796 186 100 4 78 260 40 203 1.13e-23 96.3
Msa0858560 AT5G07100.5 45.455 66 36 0 192 257 18 83 2.32e-13 68.2
Msa0858560 AT5G07100.3 32.796 186 100 4 78 260 40 203 1.13e-23 96.3
Msa0858560 AT5G07100.3 45.455 66 36 0 192 257 18 83 2.32e-13 68.2
Msa0858560 AT5G07100.1 32.796 186 100 4 78 260 133 296 5.53e-23 96.3
Msa0858560 AT5G07100.1 45.455 66 36 0 192 257 111 176 4.80e-13 68.6
Msa0858560 AT5G56270.1 47.436 78 41 0 183 260 472 549 1.10e-19 89.4
Msa0858560 AT2G03340.1 41.573 89 52 0 183 271 400 488 1.45e-18 85.5
Msa0858560 AT2G03340.1 42.857 84 47 1 189 272 241 323 3.49e-15 75.5
Msa0858560 AT1G13960.1 45.333 75 41 0 183 257 394 468 2.39e-18 85.1
Msa0858560 AT1G13960.1 46.575 73 38 1 185 257 216 287 5.95e-15 75.1
Msa0858560 AT1G13960.2 45.333 75 41 0 183 257 241 315 2.44e-18 84.3
Msa0858560 AT1G13960.2 46.575 73 38 1 185 257 63 134 7.83e-15 73.9
Msa0858560 AT2G38470.1 45.570 79 43 0 183 261 347 425 6.18e-18 84.0
Msa0858560 AT4G30935.1 29.665 209 110 6 82 258 188 391 7.55e-18 83.6
Msa0858560 AT4G26440.1 39.604 101 58 1 164 264 341 438 4.06e-17 81.6
Msa0858560 AT4G26640.5 44.872 78 43 0 183 260 190 267 1.19e-16 79.3
Msa0858560 AT4G26640.5 44.444 63 32 2 196 257 33 93 6.60e-11 62.8
Msa0858560 AT4G26640.6 44.872 78 43 0 183 260 190 267 1.19e-16 79.3
Msa0858560 AT4G26640.6 44.444 63 32 2 196 257 33 93 6.60e-11 62.8
Msa0858560 AT4G26640.4 44.872 78 43 0 183 260 190 267 1.19e-16 79.3
Msa0858560 AT4G26640.4 44.444 63 32 2 196 257 33 93 6.60e-11 62.8
Msa0858560 AT4G26640.3 44.872 78 43 0 183 260 190 267 1.19e-16 79.3
Msa0858560 AT4G26640.3 44.444 63 32 2 196 257 33 93 6.60e-11 62.8
Msa0858560 AT4G26640.7 44.872 78 43 0 183 260 326 403 1.31e-16 79.7
Msa0858560 AT4G26640.7 38.824 85 48 3 196 279 169 250 5.99e-11 63.2
Msa0858560 AT4G26640.1 44.872 78 43 0 183 260 294 371 1.40e-16 79.7
Msa0858560 AT4G26640.1 38.824 85 48 3 196 279 137 218 5.84e-11 63.2
Msa0858560 AT4G26640.2 44.872 78 43 0 183 260 366 443 1.41e-16 79.7
Msa0858560 AT4G26640.2 38.824 85 48 3 196 279 209 290 5.83e-11 63.2
Msa0858560 AT2G37260.3 26.957 230 110 5 78 254 101 325 4.37e-16 77.8
Msa0858560 AT2G37260.2 26.957 230 110 5 78 254 99 323 4.48e-16 77.4
Msa0858560 AT5G13080.1 45.588 68 37 0 187 254 56 123 3.61e-15 71.6
Msa0858560 AT5G64810.1 42.254 71 41 0 186 256 98 168 6.35e-15 72.0
Msa0858560 AT5G26170.1 44.928 69 38 0 186 254 101 169 1.24e-14 70.9
Msa0858560 AT1G55600.1 45.833 72 39 0 186 257 295 366 2.74e-14 72.8
Msa0858560 AT2G47260.1 43.421 76 43 0 183 258 159 234 5.28e-14 71.6
Msa0858560 AT5G49520.1 44.737 76 42 0 183 258 206 281 5.77e-14 71.6
Msa0858560 AT2G21900.1 40.789 76 43 1 184 257 93 168 9.04e-14 68.9
Msa0858560 AT2G44745.1 43.243 74 42 0 183 256 130 203 2.39e-13 68.2
Msa0858560 AT1G69310.4 45.714 70 38 0 186 255 135 204 2.73e-13 68.9
Msa0858560 AT1G69310.2 45.714 70 38 0 186 255 135 204 2.73e-13 68.9
Msa0858560 AT1G69310.1 45.714 70 38 0 186 255 135 204 2.73e-13 68.9
Msa0858560 AT3G62340.1 48.387 62 32 0 197 258 117 178 4.31e-13 68.2
Msa0858560 AT5G41570.1 43.478 69 39 0 186 254 86 154 7.80e-13 65.9
Msa0858560 AT1G64000.1 43.478 69 39 0 186 254 102 170 1.06e-12 65.9
Msa0858560 AT1G29860.1 42.667 75 43 0 183 257 121 195 1.36e-12 67.0
Msa0858560 AT2G25000.2 36.782 87 51 2 185 267 90 176 1.45e-12 66.2
Msa0858560 AT2G25000.4 36.782 87 51 2 185 267 90 176 1.45e-12 66.2
Msa0858560 AT3G01970.1 42.647 68 39 0 187 254 54 121 1.63e-12 64.3
Msa0858560 AT2G25000.3 36.782 87 51 2 185 267 112 198 1.84e-12 66.2
Msa0858560 AT4G18170.1 45.333 75 41 0 183 257 157 231 1.85e-12 67.0
Msa0858560 AT4G12020.5 41.096 73 42 1 185 257 388 459 2.06e-12 67.8
Msa0858560 AT4G12020.7 41.096 73 42 1 185 257 455 526 2.22e-12 67.8
Msa0858560 AT4G12020.1 41.096 73 42 1 185 257 455 526 2.22e-12 67.8
Msa0858560 AT4G12020.9 41.096 73 42 1 185 257 455 526 2.22e-12 67.8
Msa0858560 AT4G12020.10 41.096 73 42 1 185 257 455 526 2.23e-12 67.8
Msa0858560 AT4G12020.3 41.096 73 42 1 185 257 455 526 2.23e-12 67.8
Msa0858560 AT4G12020.4 41.096 73 42 1 185 257 455 526 2.23e-12 67.8
Msa0858560 AT4G12020.2 41.096 73 42 1 185 257 455 526 2.23e-12 67.8
Msa0858560 AT4G12020.6 41.096 73 42 1 185 257 455 526 2.32e-12 67.8
Msa0858560 AT4G12020.8 41.096 73 42 1 185 257 455 526 2.32e-12 67.8
Msa0858560 AT4G12020.11 41.096 73 42 1 185 257 455 526 2.38e-12 67.4
Msa0858560 AT2G25000.1 36.782 87 51 2 185 267 133 219 2.64e-12 65.9
Msa0858560 AT4G39410.1 43.243 74 42 0 183 256 208 281 3.12e-12 66.2
Msa0858560 AT2G46130.1 40.580 69 41 0 186 254 18 86 4.05e-12 62.0
Msa0858560 AT5G46350.1 48.438 64 33 0 197 260 182 245 4.75e-12 65.9
Msa0858560 AT4G23550.1 40.000 75 44 1 187 260 129 203 6.62e-12 65.1
Msa0858560 AT4G01720.1 43.662 71 39 1 188 257 229 299 7.52e-12 65.9
Msa0858560 AT2G37260.1 39.706 68 41 0 187 254 338 405 8.99e-12 65.5
Msa0858560 AT4G31800.2 37.333 75 46 1 184 257 97 171 3.07e-11 62.4
Msa0858560 AT1G68150.1 41.538 65 37 1 196 259 233 297 3.75e-11 63.2
Msa0858560 AT4G31800.1 37.333 75 46 1 184 257 162 236 4.80e-11 62.8
Msa0858560 AT1G80840.1 41.429 70 40 1 189 257 137 206 5.13e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAATAATGCGTATTAA+TGG 0.253581 5_4:-56048092 Msa0858560:CDS
TAATGTAACAACTACCTATA+AGG 0.261776 5_4:-56048174 Msa0858560:CDS
ATGATCATGTTGTCCCTTAT+AGG 0.293854 5_4:+56048160 None:intergenic
ACTTCCACACCGGTCAAATA+TGG 0.326583 5_4:+56048661 None:intergenic
AATTCATGTGAAGGAGTATT+AGG 0.352642 5_4:+56048979 None:intergenic
TGTTCTTCCTGTTGTTGAAC+AGG 0.360275 5_4:-56048695 Msa0858560:CDS
ACAATGCTTGATTGAGGATT+AGG 0.367320 5_4:+56048733 None:intergenic
GTTACATTAGTCACATTTAG+AGG 0.369899 5_4:+56048188 None:intergenic
ACAATGTACAATATATTAAG+AGG 0.384416 5_4:+56048584 None:intergenic
ACTAGAGTTGGCGTGTTAAC+TGG 0.387351 5_4:-56048533 Msa0858560:CDS
TGAATGTGTGCATTTGTAAT+AGG 0.403229 5_4:+56048837 None:intergenic
AAATTTCATTGGTCAAATGA+TGG 0.419177 5_4:-56048796 Msa0858560:CDS
TCGCTCAATCATACGTGAAA+TGG 0.436400 5_4:-56048929 Msa0858560:intron
TACTTTCGATGCGCCTATCC+AGG 0.436737 5_4:-56048242 Msa0858560:CDS
AATGTAACAACTACCTATAA+GGG 0.449073 5_4:-56048173 Msa0858560:CDS
TTGTAAATGATGCGTATCGC+TGG 0.453288 5_4:-56048469 Msa0858560:CDS
ACAGGAAGAACATGATCAAC+TGG 0.462404 5_4:+56048706 None:intergenic
GCGTATCGCTGGCGCAAATA+CGG 0.465731 5_4:-56048458 Msa0858560:CDS
ATTAGGATGATTATGCTGAT+TGG 0.483420 5_4:+56048750 None:intergenic
TGTGGTCCCTGTTCAACAAC+AGG 0.490907 5_4:+56048688 None:intergenic
TTTCACTGTGCATCCTGGAT+AGG 0.494274 5_4:+56048229 None:intergenic
TTGACCGGTGTGGAAGTTCA+AGG 0.496310 5_4:-56048655 Msa0858560:intron
AATTTCATTGGTCAAATGAT+GGG 0.502281 5_4:-56048795 Msa0858560:CDS
GTTCTTCCTGTTGTTGAACA+GGG 0.504635 5_4:-56048694 Msa0858560:CDS
AGAAACTAATGATGTAGCTA+TGG 0.532105 5_4:-56049043 Msa0858560:CDS
TTACATTAGTCACATTTAGA+GGG 0.537229 5_4:+56048189 None:intergenic
AATACTGATGCTACTAGAGT+TGG 0.539414 5_4:-56048545 Msa0858560:CDS
TGACGGACTCCCCTACCAAT+CGG 0.541659 5_4:+56048131 None:intergenic
TCATGAACCACCGATTGGTA+GGG 0.556990 5_4:-56048141 Msa0858560:CDS
CCACATCAACCATATTTGAC+CGG 0.557825 5_4:-56048670 Msa0858560:CDS
ATCATGAACCACCGATTGGT+AGG 0.562327 5_4:-56048142 Msa0858560:CDS
CATGAACCACCGATTGGTAG+GGG 0.566020 5_4:-56048140 Msa0858560:CDS
TGTAGCTATGGCTTGTGACT+TGG 0.571450 5_4:-56049031 Msa0858560:CDS
TATTGATTAGATTGTATCAG+AGG 0.571636 5_4:+56048955 None:intergenic
CATGATCATGAACCACCGAT+TGG 0.591424 5_4:-56048146 Msa0858560:CDS
ATCAGAGGTAATTCATGTGA+AGG 0.594434 5_4:+56048970 None:intergenic
CCGGTCAAATATGGTTGATG+TGG 0.605641 5_4:+56048670 None:intergenic
ACTCGATGTCGTACTTCAGA+AGG 0.611376 5_4:+56049096 None:intergenic
GGTGGTACAATGCTTGATTG+AGG 0.614087 5_4:+56048727 None:intergenic
TACATTAGTCACATTTAGAG+GGG 0.623510 5_4:+56048190 None:intergenic
GACAATGTGATCTCCCTCTG+AGG 0.634875 5_4:+56049069 None:intergenic
CACATATGCAACACTCGCAG+AGG 0.656505 5_4:-56049002 Msa0858560:CDS
TATTTGTAACAGAGTCATGA+CGG 0.659620 5_4:+56048114 None:intergenic
GGTGTAACTGAAATATTCGA+CGG 0.662056 5_4:+56048771 None:intergenic
ATCACCTTGAACTTCCACAC+CGG 0.673408 5_4:+56048651 None:intergenic
ACATCGAGTGAATCCTCAGA+GGG 0.680083 5_4:-56049082 Msa0858560:CDS
CACTTGAAGTCTGAACAACA+AGG 0.680480 5_4:+56048501 None:intergenic
TCAACCATATTTGACCGGTG+TGG 0.682556 5_4:-56048665 Msa0858560:CDS
GGAAGAACATGATCAACTGG+TGG 0.702342 5_4:+56048709 None:intergenic
CGGACTCCCCTACCAATCGG+TGG 0.703895 5_4:+56048134 None:intergenic
GACATCGAGTGAATCCTCAG+AGG 0.735585 5_4:-56049083 Msa0858560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGTACAATATATTAAG+AGG + chr5_4:56048636-56048655 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG - chr5_4:56048793-56048812 Msa0858560:CDS 20.0%
!! ACAATGTACAATATATTAAG+AGG + chr5_4:56048636-56048655 None:intergenic 20.0%
!! TTAATGGAAAAACAATTCAA+AGG - chr5_4:56048793-56048812 Msa0858560:CDS 20.0%
!! TATTGATTAGATTGTATCAG+AGG + chr5_4:56048265-56048284 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG - chr5_4:56048410-56048429 Msa0858560:intron 25.0%
! AAATTTCATTGGTCAAATGA+TGG - chr5_4:56048421-56048440 Msa0858560:intron 25.0%
! AATTTCATTGGTCAAATGAT+GGG - chr5_4:56048422-56048441 Msa0858560:intron 25.0%
! ATGAGTTCAAAATAAAAGCT+AGG + chr5_4:56048824-56048843 None:intergenic 25.0%
! TTACATTAGTCACATTTAGA+GGG + chr5_4:56049031-56049050 None:intergenic 25.0%
! TAATGTAACAACTACCTATA+AGG - chr5_4:56049043-56049062 Msa0858560:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG - chr5_4:56049044-56049063 Msa0858560:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG - chr5_4:56049125-56049144 Msa0858560:CDS 25.0%
!! TATTGATTAGATTGTATCAG+AGG + chr5_4:56048265-56048284 None:intergenic 25.0%
! GTGCAAAAAAGAAATTTCAT+TGG - chr5_4:56048410-56048429 Msa0858560:intron 25.0%
! AAATTTCATTGGTCAAATGA+TGG - chr5_4:56048421-56048440 Msa0858560:intron 25.0%
! AATTTCATTGGTCAAATGAT+GGG - chr5_4:56048422-56048441 Msa0858560:intron 25.0%
! ATGAGTTCAAAATAAAAGCT+AGG + chr5_4:56048824-56048843 None:intergenic 25.0%
! TTACATTAGTCACATTTAGA+GGG + chr5_4:56049031-56049050 None:intergenic 25.0%
! TAATGTAACAACTACCTATA+AGG - chr5_4:56049043-56049062 Msa0858560:CDS 25.0%
! AATGTAACAACTACCTATAA+GGG - chr5_4:56049044-56049063 Msa0858560:CDS 25.0%
! ACTCAAATAATGCGTATTAA+TGG - chr5_4:56049125-56049144 Msa0858560:CDS 25.0%
AGAAACTAATGATGTAGCTA+TGG - chr5_4:56048174-56048193 Msa0858560:CDS 30.0%
!! AATTCATGTGAAGGAGTATT+AGG + chr5_4:56048241-56048260 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG - chr5_4:56048311-56048330 Msa0858560:intron 30.0%
GGCTATAAGTGGAAAAAATA+TGG - chr5_4:56048322-56048341 Msa0858560:intron 30.0%
TGAATGTGTGCATTTGTAAT+AGG + chr5_4:56048383-56048402 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG + chr5_4:56048470-56048489 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG - chr5_4:56048777-56048796 Msa0858560:CDS 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG + chr5_4:56048962-56048981 None:intergenic 30.0%
TACATTAGTCACATTTAGAG+GGG + chr5_4:56049030-56049049 None:intergenic 30.0%
GTTACATTAGTCACATTTAG+AGG + chr5_4:56049032-56049051 None:intergenic 30.0%
TATTTGTAACAGAGTCATGA+CGG + chr5_4:56049106-56049125 None:intergenic 30.0%
AGAAACTAATGATGTAGCTA+TGG - chr5_4:56048174-56048193 Msa0858560:CDS 30.0%
!! AATTCATGTGAAGGAGTATT+AGG + chr5_4:56048241-56048260 None:intergenic 30.0%
TAAGAAAAGATGGCTATAAG+TGG - chr5_4:56048311-56048330 Msa0858560:intron 30.0%
GGCTATAAGTGGAAAAAATA+TGG - chr5_4:56048322-56048341 Msa0858560:intron 30.0%
TGAATGTGTGCATTTGTAAT+AGG + chr5_4:56048383-56048402 None:intergenic 30.0%
ATTAGGATGATTATGCTGAT+TGG + chr5_4:56048470-56048489 None:intergenic 30.0%
TACGGAAGAAAAATGGTTAA+TGG - chr5_4:56048777-56048796 Msa0858560:CDS 30.0%
! GAAAGTAATTTCTGGAAGAT+TGG + chr5_4:56048962-56048981 None:intergenic 30.0%
TACATTAGTCACATTTAGAG+GGG + chr5_4:56049030-56049049 None:intergenic 30.0%
GTTACATTAGTCACATTTAG+AGG + chr5_4:56049032-56049051 None:intergenic 30.0%
TATTTGTAACAGAGTCATGA+CGG + chr5_4:56049106-56049125 None:intergenic 30.0%
ATCAGAGGTAATTCATGTGA+AGG + chr5_4:56048250-56048269 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG - chr5_4:56048301-56048320 Msa0858560:intron 35.0%
GGTGTAACTGAAATATTCGA+CGG + chr5_4:56048449-56048468 None:intergenic 35.0%
ACAATGCTTGATTGAGGATT+AGG + chr5_4:56048487-56048506 None:intergenic 35.0%
AATACTGATGCTACTAGAGT+TGG - chr5_4:56048672-56048691 Msa0858560:CDS 35.0%
! GTAATTTCTGGAAGATTGGA+GGG + chr5_4:56048958-56048977 None:intergenic 35.0%
! AGTAATTTCTGGAAGATTGG+AGG + chr5_4:56048959-56048978 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG + chr5_4:56049060-56049079 None:intergenic 35.0%
ATCAGAGGTAATTCATGTGA+AGG + chr5_4:56048250-56048269 None:intergenic 35.0%
CGTGAAATGGTAAGAAAAGA+TGG - chr5_4:56048301-56048320 Msa0858560:intron 35.0%
GGTGTAACTGAAATATTCGA+CGG + chr5_4:56048449-56048468 None:intergenic 35.0%
ACAATGCTTGATTGAGGATT+AGG + chr5_4:56048487-56048506 None:intergenic 35.0%
AATACTGATGCTACTAGAGT+TGG - chr5_4:56048672-56048691 Msa0858560:CDS 35.0%
! GTAATTTCTGGAAGATTGGA+GGG + chr5_4:56048958-56048977 None:intergenic 35.0%
! AGTAATTTCTGGAAGATTGG+AGG + chr5_4:56048959-56048978 None:intergenic 35.0%
ATGATCATGTTGTCCCTTAT+AGG + chr5_4:56049060-56049079 None:intergenic 35.0%
TCGCTCAATCATACGTGAAA+TGG - chr5_4:56048288-56048307 Msa0858560:intron 40.0%
ACAGGAAGAACATGATCAAC+TGG + chr5_4:56048514-56048533 None:intergenic 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG - chr5_4:56048522-56048541 Msa0858560:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG - chr5_4:56048523-56048542 Msa0858560:CDS 40.0%
CCACATCAACCATATTTGAC+CGG - chr5_4:56048547-56048566 Msa0858560:CDS 40.0%
CACTTGAAGTCTGAACAACA+AGG + chr5_4:56048719-56048738 None:intergenic 40.0%
TTGTAAATGATGCGTATCGC+TGG - chr5_4:56048748-56048767 Msa0858560:CDS 40.0%
GCGCAAATACGGAAGAAAAA+TGG - chr5_4:56048770-56048789 Msa0858560:CDS 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG + chr5_4:56048996-56049015 None:intergenic 40.0%
TCGCTCAATCATACGTGAAA+TGG - chr5_4:56048288-56048307 Msa0858560:intron 40.0%
ACAGGAAGAACATGATCAAC+TGG + chr5_4:56048514-56048533 None:intergenic 40.0%
TGTTCTTCCTGTTGTTGAAC+AGG - chr5_4:56048522-56048541 Msa0858560:CDS 40.0%
GTTCTTCCTGTTGTTGAACA+GGG - chr5_4:56048523-56048542 Msa0858560:CDS 40.0%
CCACATCAACCATATTTGAC+CGG - chr5_4:56048547-56048566 Msa0858560:CDS 40.0%
CACTTGAAGTCTGAACAACA+AGG + chr5_4:56048719-56048738 None:intergenic 40.0%
TTGTAAATGATGCGTATCGC+TGG - chr5_4:56048748-56048767 Msa0858560:CDS 40.0%
GCGCAAATACGGAAGAAAAA+TGG - chr5_4:56048770-56048789 Msa0858560:CDS 40.0%
!!! TACTTTTTCACTGTGCATCC+TGG + chr5_4:56048996-56049015 None:intergenic 40.0%
ACTCGATGTCGTACTTCAGA+AGG + chr5_4:56048124-56048143 None:intergenic 45.0%
ACATCGAGTGAATCCTCAGA+GGG - chr5_4:56048135-56048154 Msa0858560:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG - chr5_4:56048186-56048205 Msa0858560:CDS 45.0%
GGTGGTACAATGCTTGATTG+AGG + chr5_4:56048493-56048512 None:intergenic 45.0%
GGAAGAACATGATCAACTGG+TGG + chr5_4:56048511-56048530 None:intergenic 45.0%
! CCGGTCAAATATGGTTGATG+TGG + chr5_4:56048550-56048569 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG - chr5_4:56048552-56048571 Msa0858560:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG + chr5_4:56048559-56048578 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG + chr5_4:56048569-56048588 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG - chr5_4:56048684-56048703 Msa0858560:CDS 45.0%
GGCGCATCGAAAGTAATTTC+TGG + chr5_4:56048970-56048989 None:intergenic 45.0%
TTTCACTGTGCATCCTGGAT+AGG + chr5_4:56048991-56049010 None:intergenic 45.0%
CATGATCATGAACCACCGAT+TGG - chr5_4:56049071-56049090 Msa0858560:CDS 45.0%
! ATCATGAACCACCGATTGGT+AGG - chr5_4:56049075-56049094 Msa0858560:CDS 45.0%
! TCATGAACCACCGATTGGTA+GGG - chr5_4:56049076-56049095 Msa0858560:CDS 45.0%
ACTCGATGTCGTACTTCAGA+AGG + chr5_4:56048124-56048143 None:intergenic 45.0%
ACATCGAGTGAATCCTCAGA+GGG - chr5_4:56048135-56048154 Msa0858560:CDS 45.0%
! TGTAGCTATGGCTTGTGACT+TGG - chr5_4:56048186-56048205 Msa0858560:CDS 45.0%
GGTGGTACAATGCTTGATTG+AGG + chr5_4:56048493-56048512 None:intergenic 45.0%
GGAAGAACATGATCAACTGG+TGG + chr5_4:56048511-56048530 None:intergenic 45.0%
! CCGGTCAAATATGGTTGATG+TGG + chr5_4:56048550-56048569 None:intergenic 45.0%
TCAACCATATTTGACCGGTG+TGG - chr5_4:56048552-56048571 Msa0858560:CDS 45.0%
ACTTCCACACCGGTCAAATA+TGG + chr5_4:56048559-56048578 None:intergenic 45.0%
ATCACCTTGAACTTCCACAC+CGG + chr5_4:56048569-56048588 None:intergenic 45.0%
ACTAGAGTTGGCGTGTTAAC+TGG - chr5_4:56048684-56048703 Msa0858560:CDS 45.0%
GGCGCATCGAAAGTAATTTC+TGG + chr5_4:56048970-56048989 None:intergenic 45.0%
TTTCACTGTGCATCCTGGAT+AGG + chr5_4:56048991-56049010 None:intergenic 45.0%
CATGATCATGAACCACCGAT+TGG - chr5_4:56049071-56049090 Msa0858560:CDS 45.0%
! ATCATGAACCACCGATTGGT+AGG - chr5_4:56049075-56049094 Msa0858560:CDS 45.0%
! TCATGAACCACCGATTGGTA+GGG - chr5_4:56049076-56049095 Msa0858560:CDS 45.0%
GACATCGAGTGAATCCTCAG+AGG - chr5_4:56048134-56048153 Msa0858560:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG + chr5_4:56048151-56048170 None:intergenic 50.0%
CACATATGCAACACTCGCAG+AGG - chr5_4:56048215-56048234 Msa0858560:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG + chr5_4:56048532-56048551 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG - chr5_4:56048562-56048581 Msa0858560:CDS 50.0%
TACTTTCGATGCGCCTATCC+AGG - chr5_4:56048975-56048994 Msa0858560:CDS 50.0%
! CATGAACCACCGATTGGTAG+GGG - chr5_4:56049077-56049096 Msa0858560:CDS 50.0%
GACATCGAGTGAATCCTCAG+AGG - chr5_4:56048134-56048153 Msa0858560:CDS 50.0%
GACAATGTGATCTCCCTCTG+AGG + chr5_4:56048151-56048170 None:intergenic 50.0%
CACATATGCAACACTCGCAG+AGG - chr5_4:56048215-56048234 Msa0858560:CDS 50.0%
TGTGGTCCCTGTTCAACAAC+AGG + chr5_4:56048532-56048551 None:intergenic 50.0%
TTGACCGGTGTGGAAGTTCA+AGG - chr5_4:56048562-56048581 Msa0858560:CDS 50.0%
TACTTTCGATGCGCCTATCC+AGG - chr5_4:56048975-56048994 Msa0858560:CDS 50.0%
! CATGAACCACCGATTGGTAG+GGG - chr5_4:56049077-56049096 Msa0858560:CDS 50.0%
GCGTATCGCTGGCGCAAATA+CGG - chr5_4:56048759-56048778 Msa0858560:CDS 55.0%
!! TGACGGACTCCCCTACCAAT+CGG + chr5_4:56049089-56049108 None:intergenic 55.0%
GCGTATCGCTGGCGCAAATA+CGG - chr5_4:56048759-56048778 Msa0858560:CDS 55.0%
!! TGACGGACTCCCCTACCAAT+CGG + chr5_4:56049089-56049108 None:intergenic 55.0%
CGGACTCCCCTACCAATCGG+TGG + chr5_4:56049086-56049105 None:intergenic 65.0%
CGGACTCCCCTACCAATCGG+TGG + chr5_4:56049086-56049105 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 56048082 56049157 56048082 ID=Msa0858560;Name=Msa0858560
chr5_4 mRNA 56048082 56049157 56048082 ID=Msa0858560-mRNA-1;Parent=Msa0858560;Name=Msa0858560-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|0|0|1|0.33|0.25|4|0|279
chr5_4 exon 56048930 56049157 56048930 ID=Msa0858560-mRNA-1:exon:17143;Parent=Msa0858560-mRNA-1
chr5_4 exon 56048656 56048878 56048656 ID=Msa0858560-mRNA-1:exon:17142;Parent=Msa0858560-mRNA-1
chr5_4 exon 56048425 56048626 56048425 ID=Msa0858560-mRNA-1:exon:17141;Parent=Msa0858560-mRNA-1
chr5_4 exon 56048082 56048268 56048082 ID=Msa0858560-mRNA-1:exon:17140;Parent=Msa0858560-mRNA-1
chr5_4 CDS 56048930 56049157 56048930 ID=Msa0858560-mRNA-1:cds;Parent=Msa0858560-mRNA-1
chr5_4 CDS 56048656 56048878 56048656 ID=Msa0858560-mRNA-1:cds;Parent=Msa0858560-mRNA-1
chr5_4 CDS 56048425 56048626 56048425 ID=Msa0858560-mRNA-1:cds;Parent=Msa0858560-mRNA-1
chr5_4 CDS 56048082 56048268 56048082 ID=Msa0858560-mRNA-1:cds;Parent=Msa0858560-mRNA-1
Gene Sequence

>Msa0858560

ATGGTTTTGTCACTACAAAATGTTGATCACAATGATTCTCCTTCTGAAGTACGACATCGAGTGAATCCTCAGAGGGAGATCACATTGTCACGAGAAACTAATGATGTAGCTATGGCTTGTGACTTGGTATTATCACATATGCAACACTCGCAGAGGCCTAATACTCCTTCACATGAATTACCTCTGATACAATCTAATCAATACACTCGCTCAATCATACGTGAAATGAAGAATGAACATAAAAGAGCCTATTACAAATGCACACATTCAGACTGTCGTGCAAAAAAGAAATTTCATTGGTCAAATGATGGGACCGTCGAATATTTCAGTTACACCAATCAGCATAATCATCCTAATCCTCAATCAAGCATTGTACCACCAGTTGATCATGTTCTTCCTGTTGTTGAACAGGGACCACATCAACCATATTTGACCGGTGTGGAAGTTCAAGTTTCAATATTACATGAAAAACCTCTTAATATATTGTACATTGTTGTGCATGCAGACAACAATACTGATGCTACTAGAGTTGGCGTGTTAACTGGTGAACTTCACCTTGTTGTTCAGACTTCAAGTGCAAATGAAGTTGTAAATGATGCGTATCGCTGGCGCAAATACGGAAGAAAAATGGTTAATGGAAAAACAATTCAAAGAAATTACTTTCGATGCGCCTATCCAGGATGCACAGTGAAAAAGTATGTTGAAAAGTCCCCTCTAAATGTGACTAATGTAACAACTACCTATAAGGGACAACATGATCATGAACCACCGATTGGTAGGGGAGTCCGTCATGACTCTGTTACAAATACTCAAATAATGCGTATTAATGGCGAGCCATAA
Protein sequence

>Msa0858560

MVLSLQNVDHNDSPSEVRHRVNPQREITLSRETNDVAMACDLVLSHMQHSQRPNTPSHELPLIQSNQYTRSIIREMKNEHKRAYYKCTHSDCRAKKKFHWSNDGTVEYFSYTNQHNHPNPQSSIVPPVDHVLPVVEQGPHQPYLTGVEVQVSILHEKPLNILYIVVHADNNTDATRVGVLTGELHLVVQTSSANEVVNDAYRWRKYGRKMVNGKTIQRNYFRCAYPGCTVKKYVEKSPLNVTNVTTTYKGQHDHEPPIGRGVRHDSVTNTQIMRINGEP*