AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0858630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0858630 MtrunA17_Chr1g0203051 45.527 626 325 3 6 624 5 621 0.0 586
Msa0858630 MtrunA17_Chr2g0287651 35.452 708 438 5 73 774 66 760 1.32e-148 457
Msa0858630 MtrunA17_Chr2g0308841 68.841 276 69 2 411 686 1 259 1.30e-132 394
Msa0858630 MtrunA17_Chr5g0448761 32.042 568 374 7 84 640 62 628 2.40e-100 328
Msa0858630 MtrunA17_Chr1g0202341 30.195 563 380 6 83 637 52 609 3.10e-88 293
Msa0858630 MtrunA17_Chr2g0324011 28.007 582 396 9 88 656 16 587 1.31e-78 265
Msa0858630 MtrunA17_Chr8g0363841 28.763 598 395 9 55 640 2 580 6.00e-73 251
Msa0858630 MtrunA17_Chr5g0441581 27.258 609 403 14 88 678 38 624 2.41e-71 247
Msa0858630 MtrunA17_Chr1g0198761 26.944 720 467 20 88 772 53 748 3.83e-70 245
Msa0858630 MtrunA17_Chr2g0296781 29.167 552 367 12 97 634 12 553 7.19e-70 244
Msa0858630 MtrunA17_Chr1g0203721 28.169 568 377 12 88 637 69 623 3.47e-67 239
Msa0858630 MtrunA17_Chr1g0207551 28.470 562 380 12 87 637 39 589 2.18e-66 236
Msa0858630 MtrunA17_Chr5g0448511 29.000 400 276 4 247 640 3 400 3.20e-66 229
Msa0858630 MtrunA17_Chr2g0287061 28.620 594 364 16 59 640 159 704 6.27e-66 234
Msa0858630 MtrunA17_Chr5g0440451 27.121 601 392 11 88 662 49 629 9.25e-66 236
Msa0858630 MtrunA17_Chr3g0118611 29.263 434 295 4 238 662 96 526 1.03e-65 233
Msa0858630 MtrunA17_Chr6g0477261 31.293 441 286 8 208 634 5 442 1.75e-64 228
Msa0858630 MtrunA17_Chr6g0458391 24.519 624 425 13 33 637 169 765 9.50e-64 228
Msa0858630 MtrunA17_Chr6g0458391 35.714 126 70 4 87 208 96 214 2.04e-12 70.9
Msa0858630 MtrunA17_Chr3g0082121 25.627 558 393 7 87 637 48 590 2.68e-60 217
Msa0858630 MtrunA17_Chr3g0080351 26.889 569 372 12 87 637 81 623 4.07e-57 210
Msa0858630 MtrunA17_Chr1g0196541 26.032 557 384 10 97 637 12 556 7.11e-57 207
Msa0858630 MtrunA17_Chr5g0431001 29.691 421 267 8 88 486 67 480 8.12e-57 202
Msa0858630 MtrunA17_Chr2g0276841 27.160 567 387 13 88 639 76 631 1.12e-55 204
Msa0858630 MtrunA17_Chr2g0290391 27.972 572 369 14 88 634 57 610 6.84e-55 201
Msa0858630 MtrunA17_Chr2g0291581 25.355 564 384 13 87 634 76 618 7.27e-54 198
Msa0858630 MtrunA17_Chr2g0291591 26.575 508 350 11 83 579 65 560 5.20e-53 193
Msa0858630 MtrunA17_Chr2g0329031 23.750 560 399 9 87 636 19 560 1.33e-50 189
Msa0858630 MtrunA17_Chr2g0294841 26.889 569 376 16 88 632 51 603 7.05e-46 176
Msa0858630 MtrunA17_Chr1g0164591 27.243 301 207 2 174 469 14 307 1.89e-37 143
Msa0858630 MtrunA17_Chr1g0171741 40.491 163 93 2 50 212 104 262 1.04e-31 124
Msa0858630 MtrunA17_Chr8g0389811 43.307 127 72 0 67 193 69 195 3.67e-30 118
Msa0858630 MtrunA17_Chr2g0331371 48.780 123 63 0 64 186 37 159 3.24e-29 115
Msa0858630 MtrunA17_Chr7g0258861 27.148 291 198 7 88 370 16 300 7.51e-28 114
Msa0858630 MtrunA17_Chr6g0473421 47.368 95 50 0 64 158 37 131 1.22e-24 100
Msa0858630 MtrunA17_Chr1g0172931 36.508 126 80 0 39 164 33 158 1.08e-19 86.7
Msa0858630 MtrunA17_Chr8g0340801 36.190 105 67 0 193 297 1 105 1.08e-18 82.0
Msa0858630 MtrunA17_Chr1g0149131 28.696 115 67 3 361 465 1 110 4.11e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0858630 AT4G38180.1 30.820 610 394 8 39 640 48 637 5.86e-96 317
Msa0858630 AT3G06250.2 29.749 558 337 13 87 637 195 704 1.23e-73 256
Msa0858630 AT3G06250.1 29.749 558 337 13 87 637 195 704 1.23e-73 256
Msa0858630 AT3G06250.3 29.749 558 337 13 87 637 195 704 1.23e-73 256
Msa0858630 AT5G18960.2 29.803 557 341 15 87 637 216 728 6.35e-69 243
Msa0858630 AT5G18960.1 29.803 557 341 15 87 637 216 728 6.35e-69 243
Msa0858630 AT4G15090.1 26.821 604 409 11 87 675 56 641 1.20e-68 243
Msa0858630 AT4G15090.5 26.821 604 409 11 87 675 56 641 1.20e-68 243
Msa0858630 AT4G15090.3 26.821 604 409 11 87 675 56 641 1.20e-68 243
Msa0858630 AT4G15090.2 26.821 604 409 11 87 675 56 641 1.20e-68 243
Msa0858630 AT4G15090.4 26.821 604 409 11 87 675 56 641 1.20e-68 243
Msa0858630 AT2G27110.2 26.367 622 406 12 24 640 8 582 4.83e-66 236
Msa0858630 AT2G27110.1 26.367 622 406 12 24 640 8 582 4.83e-66 236
Msa0858630 AT2G27110.3 29.040 396 273 3 250 640 45 437 1.89e-62 223
Msa0858630 AT3G22170.2 27.083 576 363 14 87 637 75 618 7.37e-60 218
Msa0858630 AT3G22170.1 27.083 576 363 14 87 637 75 618 7.37e-60 218
Msa0858630 AT1G80010.1 28.621 580 387 13 87 647 100 671 9.64e-60 216
Msa0858630 AT1G10240.2 28.546 564 377 14 88 636 54 606 2.58e-59 214
Msa0858630 AT1G10240.1 28.546 564 377 14 88 636 54 606 2.58e-59 214
Msa0858630 AT1G76320.3 27.046 562 382 11 87 638 2 545 2.83e-58 211
Msa0858630 AT1G76320.2 27.046 562 382 11 87 638 2 545 3.00e-58 212
Msa0858630 AT1G76320.1 27.046 562 382 11 87 638 2 545 3.33e-58 212
Msa0858630 AT1G76320.4 27.046 562 382 11 87 638 2 545 3.33e-58 212
Msa0858630 AT1G52520.1 26.412 602 400 16 87 664 90 672 1.65e-57 209
Msa0858630 AT4G38170.1 28.767 365 251 4 279 637 38 399 3.31e-53 194
Msa0858630 AT2G32250.8 25.090 554 363 11 87 634 2 509 8.75e-51 191
Msa0858630 AT2G32250.2 25.090 554 363 11 87 634 43 550 8.79e-51 191
Msa0858630 AT2G32250.4 25.090 554 363 11 87 634 43 550 8.79e-51 191
Msa0858630 AT2G32250.6 25.090 554 363 11 87 634 43 550 8.86e-51 191
Msa0858630 AT2G32250.1 25.090 554 363 11 87 634 43 550 8.86e-51 191
Msa0858630 AT2G32250.3 25.090 554 363 11 87 634 43 550 8.86e-51 191
Msa0858630 AT2G32250.7 25.090 554 363 11 87 634 2 509 9.09e-51 191
Msa0858630 AT2G32250.5 25.090 554 363 11 87 634 43 550 9.79e-51 191
Msa0858630 AT5G28530.3 27.193 570 376 15 88 634 61 614 7.27e-50 187
Msa0858630 AT5G28530.2 27.193 570 376 15 88 634 61 614 7.27e-50 187
Msa0858630 AT5G28530.1 26.842 570 382 15 88 634 61 618 8.05e-49 184
Msa0858630 AT4G19990.5 25.000 576 365 14 87 637 26 559 3.74e-47 180
Msa0858630 AT4G19990.3 25.000 576 365 14 87 637 26 559 4.37e-47 180
Msa0858630 AT4G19990.4 25.000 576 365 14 87 637 26 559 4.37e-47 180
Msa0858630 AT4G19990.7 25.000 576 365 14 87 637 40 573 4.44e-47 180
Msa0858630 AT4G19990.6 24.451 638 413 15 87 699 26 619 5.50e-47 179
Msa0858630 AT4G19990.2 24.451 638 413 15 87 699 26 619 5.50e-47 179
Msa0858630 AT4G19990.1 25.138 545 336 14 120 637 3 502 6.46e-44 169
Msa0858630 AT5G28530.5 24.910 558 341 16 88 634 61 551 2.53e-32 134
Msa0858630 AT5G28530.4 24.552 558 347 16 88 634 61 555 2.66e-31 130

Find 165 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGCTTTGGCAGATGCTTC+TGG 0.082781 5_4:-56224847 Msa0858630:CDS
TATAGCCCATTGACATTTCT+TGG 0.207487 5_4:+56223828 None:intergenic
CATCACAGGCTGACCATAAT+TGG 0.240920 5_4:+56223964 None:intergenic
ATGCCAATGGATCTCTATAT+TGG 0.244219 5_4:-56223583 Msa0858630:CDS
CAACCGTGTTGTTGATTATC+TGG 0.246014 5_4:-56223474 Msa0858630:CDS
TTTGTTCATGTCAAGGTTAC+AGG 0.251328 5_4:-56225131 Msa0858630:CDS
GGGCTTTGGCAGATGCTTCT+GGG 0.266190 5_4:-56224846 Msa0858630:CDS
TCTGAAATGGTTCAAAAGTT+TGG 0.269335 5_4:-56224220 Msa0858630:CDS
TTTGATTACCAATCCAATTA+TGG 0.278288 5_4:-56223977 Msa0858630:CDS
CAAATTGCCGCAAGGTATTC+AGG 0.289511 5_4:-56223554 Msa0858630:CDS
CTAGGCAAAACCAAACATTT+TGG 0.311764 5_4:+56223644 None:intergenic
CTGAAATGGTTCAAAAGTTT+GGG 0.313722 5_4:-56224219 Msa0858630:CDS
TTCAAATAAGACATGAAATC+AGG 0.313774 5_4:+56224292 None:intergenic
TGGGACAATACGTGCATTCT+AGG 0.314589 5_4:-56224069 Msa0858630:CDS
GATCACAATCATGCTATGTT+AGG 0.329562 5_4:-56224976 Msa0858630:CDS
AACTGAAAATCCTGACATTC+TGG 0.342907 5_4:+56225209 None:intergenic
TGAAGATCTTCCAAAATGTT+TGG 0.345878 5_4:-56223654 Msa0858630:CDS
ATCAGAGGAAGTTTCTTTGC+CGG 0.355305 5_4:-56224133 Msa0858630:CDS
TGAAGAACTTTGGTCTGAAA+TGG 0.356814 5_4:-56224233 Msa0858630:CDS
GGAGGCTTGAGCGGCTGTTC+TGG 0.359878 5_4:-56224663 Msa0858630:CDS
GTACGGAACATAAGAAATAT+TGG 0.363517 5_4:+56223890 None:intergenic
TGCCAATGGATCTCTATATT+GGG 0.365417 5_4:-56223582 Msa0858630:CDS
TCCGATTATGATAAAAGATA+TGG 0.367150 5_4:-56225334 Msa0858630:CDS
AATTGAATCTTTAGCAAACT+TGG 0.371976 5_4:+56223616 None:intergenic
TTGCCTACATGTTCTACTCT+TGG 0.376762 5_4:-56225236 Msa0858630:CDS
GCAGTGATGGAAGTGCCTTT+TGG 0.377707 5_4:-56222904 Msa0858630:CDS
AAACAATGATTTACGTTGCT+TGG 0.383660 5_4:+56224774 None:intergenic
TCTTGGTTTGCCAGAATGTC+AGG 0.390875 5_4:-56225219 Msa0858630:CDS
CACGAACATTTAAAGACAAC+TGG 0.392495 5_4:+56223743 None:intergenic
CAGCTTCTCACAAACATTAG+AGG 0.393690 5_4:+56224700 None:intergenic
TTAACAAATTTGAAGAACTT+TGG 0.394832 5_4:-56224243 Msa0858630:CDS
TTTGCTAAAGATTCAATTTG+TGG 0.401332 5_4:-56223611 Msa0858630:CDS
CAAAATATTGTGGTGATGGT+AGG 0.405059 5_4:-56223793 Msa0858630:CDS
CACACCACAGGTTGTGTGCT+TGG 0.405584 5_4:-56224351 Msa0858630:CDS
CAACCATACGTATGTTTCTT+CGG 0.408075 5_4:+56224477 None:intergenic
TGCAGCTTGTGCGGTGTAAT+AGG 0.410487 5_4:-56223281 Msa0858630:CDS
TCAAGGTTACAGGGAGGATA+GGG 0.412309 5_4:-56225121 Msa0858630:CDS
GTACCAAATGTATGGGGCTT+TGG 0.414713 5_4:-56224860 Msa0858630:CDS
CTGCCAAAGCCCCATACATT+TGG 0.417656 5_4:+56224857 None:intergenic
GGTAATCAAATAAAACTTCC+CGG 0.420822 5_4:+56223990 None:intergenic
TAAATGTTCGTGTCTTAGAA+TGG 0.428935 5_4:-56223732 Msa0858630:CDS
TGTCCGTTTGTTGTGTTCTC+AGG 0.434603 5_4:-56224556 Msa0858630:CDS
CAGCCACGACTTTACACATC+TGG 0.436929 5_4:+56223525 None:intergenic
CGCAAACCAGTCTGCATCAC+AGG 0.437325 5_4:+56223950 None:intergenic
TCCATGCTGTTCTGCACCAC+TGG 0.439550 5_4:+56223395 None:intergenic
CAGCATGGACGAGGATGTCT+TGG 0.444143 5_4:-56223381 Msa0858630:CDS
GTCTTAGAATGGAATCCCTT+GGG 0.450708 5_4:-56223721 Msa0858630:CDS
GGAAGTTCAAATTCAGTTGA+AGG 0.452477 5_4:-56225384 Msa0858630:CDS
GGAATGCTTCAACCAACATT+GGG 0.455845 5_4:-56224318 Msa0858630:CDS
TGCAAAATTTCGAGTCCACT+TGG 0.456820 5_4:-56225040 Msa0858630:CDS
TTCAAATTCAGTTGAAGGCT+CGG 0.457292 5_4:-56225379 Msa0858630:CDS
TGTCTTAGAATGGAATCCCT+TGG 0.459409 5_4:-56223722 Msa0858630:CDS
TGCTTACCCAAGCGGTACTC+CGG 0.461915 5_4:-56223327 Msa0858630:CDS
ATAAGACCGTACCAAATGTA+TGG 0.463653 5_4:-56224868 Msa0858630:CDS
GGCTTTGGCAGATGCTTCTG+GGG 0.464116 5_4:-56224845 Msa0858630:CDS
CTTCCGATACGTTGCATCGA+AGG 0.469074 5_4:+56224591 None:intergenic
GTCTACCCGGAGTACCGCTT+GGG 0.470254 5_4:+56223321 None:intergenic
TCTTGGACCCACAGTGTGTA+AGG 0.475811 5_4:-56223364 Msa0858630:CDS
ACCTTTGTTTGTTCATGTCA+AGG 0.486075 5_4:-56225138 Msa0858630:CDS
AGGAATGCTTCAACCAACAT+TGG 0.487173 5_4:-56224319 Msa0858630:CDS
CGTCTACCCGGAGTACCGCT+TGG 0.491249 5_4:+56223320 None:intergenic
CTGAACATTGATGCGAATCA+TGG 0.491252 5_4:+56223235 None:intergenic
ATGAACAAACAAAGGTCTGC+TGG 0.497463 5_4:+56225145 None:intergenic
AAAATATTGTGGTGATGGTA+GGG 0.498943 5_4:-56223792 Msa0858630:CDS
TGTGTGCTTGGCATCTGTTA+AGG 0.501921 5_4:-56224339 Msa0858630:CDS
GAAGCTGTATTGACGATGAT+AGG 0.508891 5_4:-56224684 Msa0858630:CDS
GTTTCAAAATATTGTGGTGA+TGG 0.514200 5_4:-56223797 Msa0858630:CDS
TTGCAGATGGATTGCAGTGA+TGG 0.514520 5_4:-56222917 Msa0858630:intron
GTCAAGGTTACAGGGAGGAT+AGG 0.518923 5_4:-56225122 Msa0858630:CDS
AGATAGCCAAGAAATGTCAA+TGG 0.519655 5_4:-56223834 Msa0858630:CDS
GAGCGGCTGTTCTGGTGTGA+CGG 0.521233 5_4:-56224655 Msa0858630:CDS
TTCACGGTTTCAAAATATTG+TGG 0.522903 5_4:-56223803 Msa0858630:CDS
ATTTGTGGTAGTTATGCCAA+TGG 0.523957 5_4:-56223596 Msa0858630:CDS
GGTCAGCCTGTGATGCAGAC+TGG 0.523993 5_4:-56223956 Msa0858630:CDS
GCTTTGGCAGATGCTTCTGG+GGG 0.524995 5_4:-56224844 Msa0858630:CDS
AGAAACATACGTATGGTTGT+TGG 0.527629 5_4:-56224473 Msa0858630:CDS
CAGGCTGACCATAATTGGAT+TGG 0.528137 5_4:+56223969 None:intergenic
ACGTGACGTGGTTCTTATAC+CGG 0.529617 5_4:+56224114 None:intergenic
AACCGTGTTGTTGATTATCT+GGG 0.531879 5_4:-56223473 Msa0858630:CDS
GATAGCCAAGAAATGTCAAT+GGG 0.534303 5_4:-56223833 Msa0858630:CDS
ATTTACGTTGCTTGGCAACC+TGG 0.534644 5_4:+56224782 None:intergenic
TTGATCGGCTGAAATTGAAA+AGG 0.535702 5_4:-56223442 Msa0858630:CDS
CACAACCTGTGGTGTGCTGC+AGG 0.536485 5_4:+56224358 None:intergenic
CGTATTGTCCCATGTGGCTG+TGG 0.537113 5_4:+56224080 None:intergenic
TAAGACCGTACCAAATGTAT+GGG 0.537974 5_4:-56224867 Msa0858630:CDS
ACACCTGAGAACACAACAAA+CGG 0.540110 5_4:+56224553 None:intergenic
AAAACCCTGCAAAACCCAAA+AGG 0.542937 5_4:+56222889 None:intergenic
ACACCATCGCGCTCTAACAA+AGG 0.543044 5_4:+56225570 None:intergenic
TTGGGTTAGAAGACAACACT+TGG 0.543866 5_4:-56224201 Msa0858630:CDS
CCTTTGTCCTTACACACTGT+GGG 0.544424 5_4:+56223357 None:intergenic
CCACAGTGTGTAAGGACAAA+GGG 0.549776 5_4:-56223356 Msa0858630:CDS
ATTGAGCGTAGTCGAAAAGC+TGG 0.551197 5_4:-56224892 Msa0858630:CDS
TGGCCTTCGATGCAACGTAT+CGG 0.553496 5_4:-56224594 Msa0858630:CDS
CACAATCATGCTATGTTAGG+AGG 0.553509 5_4:-56224973 Msa0858630:CDS
ATCCCAATATAGAGATCCAT+TGG 0.553790 5_4:+56223580 None:intergenic
TTGTTCATGTCAAGGTTACA+GGG 0.556666 5_4:-56225130 Msa0858630:CDS
TGAGTACGTTTGCGTCTACC+CGG 0.558270 5_4:+56223308 None:intergenic
GAAATCAGGAATCCCAATGT+TGG 0.562787 5_4:+56224306 None:intergenic
AAGAACCACGTCACGTTGTG+AGG 0.565014 5_4:-56224107 Msa0858630:CDS
AGAAATGGACGGACGTTCAG+AGG 0.566572 5_4:-56225601 None:intergenic
TCGTAAGGGGCAAGTGGTGA+GGG 0.567327 5_4:-56225187 Msa0858630:CDS
GCTTACCCAAGCGGTACTCC+GGG 0.567746 5_4:-56223326 Msa0858630:CDS
ATTGTGGTGATGGTAGGGTT+TGG 0.568535 5_4:-56223787 Msa0858630:CDS
ACGCTCAATTTCAGCTATGT+CGG 0.568884 5_4:+56224906 None:intergenic
TTCGTAAGGGGCAAGTGGTG+AGG 0.571006 5_4:-56225188 Msa0858630:CDS
TGTATGAAAAGAAGAAGATG+TGG 0.574681 5_4:-56224168 Msa0858630:CDS
TTCAGTTCGTAAGGGGCAAG+TGG 0.578338 5_4:-56225193 Msa0858630:CDS
CCTTTGTCAGTTATAACAGA+TGG 0.578591 5_4:-56224418 Msa0858630:CDS
TGATATAATGAAGATTGACC+TGG 0.579924 5_4:-56225310 Msa0858630:CDS
ATGCATGTTGGCTGACATTG+AGG 0.584855 5_4:-56224266 Msa0858630:CDS
ATCTGGGTGGTGAAATTGAT+CGG 0.585047 5_4:-56223457 Msa0858630:CDS
CAAGTGGTGAGGGATCGAAA+TGG 0.589736 5_4:-56225177 Msa0858630:CDS
GGTGCAGAACAGCATGGACG+AGG 0.595156 5_4:-56223390 Msa0858630:CDS
TATGATCACATGGCAACCCA+AGG 0.596533 5_4:+56223705 None:intergenic
TGCGACTGGTATAGTAACAC+GGG 0.597035 5_4:-56224503 Msa0858630:CDS
CCCTTTGTCCTTACACACTG+TGG 0.599568 5_4:+56223356 None:intergenic
CCCACAGTGTGTAAGGACAA+AGG 0.609140 5_4:-56223357 Msa0858630:CDS
CAAGGTTACAGGGAGGATAG+GGG 0.609406 5_4:-56225120 Msa0858630:CDS
TGAGGCACTCCACAGCCACA+TGG 0.615499 5_4:-56224089 Msa0858630:CDS
AAGGTTACAGGGAGGATAGG+GGG 0.615842 5_4:-56225119 Msa0858630:CDS
TCCAGTGGTGCAGAACAGCA+TGG 0.619624 5_4:-56223396 Msa0858630:CDS
ACATTGATGCGAATCATGGA+GGG 0.620091 5_4:+56223239 None:intergenic
AAAGCCCCATACATTTGGTA+CGG 0.620279 5_4:+56224862 None:intergenic
GCTGTATTGACGATGATAGG+AGG 0.620500 5_4:-56224681 Msa0858630:CDS
GTGTAAAGTCGTGGCTGAGA+TGG 0.622468 5_4:-56223519 Msa0858630:CDS
TTTGTATTCAGAGAAATGGA+CGG 0.623463 5_4:-56225612 None:intergenic
ACAAATTGCGAACAAAGCAA+TGG 0.627176 5_4:-56225417 Msa0858630:CDS
AACATTGATGCGAATCATGG+AGG 0.628591 5_4:+56223238 None:intergenic
TGGGCCACTGCTCACATCAG+AGG 0.629270 5_4:-56224148 Msa0858630:CDS
CCATCTGTTATAACTGACAA+AGG 0.632179 5_4:+56224418 None:intergenic
TTGCGACTGGTATAGTAACA+CGG 0.632252 5_4:-56224504 Msa0858630:CDS
GACTATGCCTGAATACCTTG+CGG 0.633998 5_4:+56223547 None:intergenic
CAAAGCAATGGAGATGTGAG+TGG 0.636953 5_4:-56225405 Msa0858630:CDS
ACTCAGACTTGCAGCTTGTG+CGG 0.638133 5_4:-56223290 Msa0858630:CDS
GGGATTCACAAATTGCCGCA+AGG 0.642439 5_4:-56223562 Msa0858630:CDS
ACCTTGACATGAACAAACAA+AGG 0.644270 5_4:+56225137 None:intergenic
AGACCGAAGAAACATACGTA+TGG 0.644828 5_4:-56224480 Msa0858630:CDS
AAACCAAGAGTAGAACATGT+AGG 0.647789 5_4:+56225233 None:intergenic
ACTTCCTCTGATGTGAGCAG+TGG 0.650809 5_4:+56224144 None:intergenic
TTGTTACCCTCAGTTGACAT+CGG 0.651830 5_4:+56223200 None:intergenic
AGTCCAGATGTGTAAAGTCG+TGG 0.653679 5_4:-56223528 Msa0858630:CDS
AATCCTTTGTTAGAGCGCGA+TGG 0.654133 5_4:-56225573 Msa0858630:CDS
GATGCCAAGCACACAACCTG+TGG 0.654695 5_4:+56224347 None:intergenic
GAGGCACTCCACAGCCACAT+GGG 0.656832 5_4:-56224088 Msa0858630:CDS
GCCTTGACAAAATATCCAAG+TGG 0.657432 5_4:+56225025 None:intergenic
ACAGTCACCGATGTCAACTG+AGG 0.659122 5_4:-56223207 Msa0858630:CDS
CGTGTTGTTGATTATCTGGG+TGG 0.659880 5_4:-56223470 Msa0858630:CDS
GTATGAAAAGAAGAAGATGT+GGG 0.660182 5_4:-56224167 Msa0858630:CDS
GGTTGTGGTGCTTACCCAAG+CGG 0.663165 5_4:-56223335 Msa0858630:CDS
CACCCAGATAATCAACAACA+CGG 0.664460 5_4:+56223471 None:intergenic
ATGATCACATGGCAACCCAA+GGG 0.665385 5_4:+56223706 None:intergenic
TCTGTTATAACTGACAAAGG+AGG 0.671475 5_4:+56224421 None:intergenic
CAGTCACCGATGTCAACTGA+GGG 0.677228 5_4:-56223206 Msa0858630:CDS
GAGTGCCTCACAACGTGACG+TGG 0.682576 5_4:+56224102 None:intergenic
ATAGCAAGAATATGATCACA+TGG 0.684248 5_4:+56223695 None:intergenic
ATGTTAGGAGGTACTCTGTG+TGG 0.688101 5_4:-56224961 Msa0858630:CDS
AAATTCAGTTGAAGGCTCGG+AGG 0.688759 5_4:-56225376 Msa0858630:CDS
TTAGCAAACTTGGACCACCT+AGG 0.692699 5_4:+56223626 None:intergenic
CGTGGCTGAGATGGTCTACG+AGG 0.697189 5_4:-56223510 Msa0858630:CDS
ACGATGATAGGAGGCTTGAG+CGG 0.698402 5_4:-56224672 Msa0858630:CDS
AAGACCGTACCAAATGTATG+GGG 0.707217 5_4:-56224866 Msa0858630:CDS
TTCATGTCAAGGTTACAGGG+AGG 0.709283 5_4:-56225127 Msa0858630:CDS
TGTGTAAGGACAAAGGGTTG+TGG 0.711066 5_4:-56223350 Msa0858630:CDS
GAACTGTGATTCAGTTCCAG+TGG 0.715411 5_4:-56223411 Msa0858630:CDS
AATGCACGTATTGTCCCATG+TGG 0.761302 5_4:+56224074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAATGCATAAATTAA+TGG + chr5_4:56225511-56225530 None:intergenic 10.0%
!! TTTATTAATATGTGTTCATT+AGG - chr5_4:56225364-56225383 Msa0858630:CDS 15.0%
!!! ATGTGAAATTTGAAATTGAA+TGG - chr5_4:56222960-56222979 Msa0858630:intron 20.0%
!!! TTTTAAATTAACTTAGCATG+TGG - chr5_4:56222985-56223004 Msa0858630:intron 20.0%
!!! CTAAAAGAAAATTTTCTTAC+AGG - chr5_4:56223008-56223027 Msa0858630:intron 20.0%
!!! ACCATATCTTTTATCATAAT+CGG + chr5_4:56223150-56223169 None:intergenic 20.0%
!! AATGAAAAATGCCATTAAAA+AGG - chr5_4:56224094-56224113 Msa0858630:CDS 20.0%
!! TTATTTGAAAAAATGCATGT+TGG - chr5_4:56224202-56224221 Msa0858630:CDS 20.0%
!! TTAACAAATTTGAAGAACTT+TGG - chr5_4:56224237-56224256 Msa0858630:CDS 20.0%
! TCCGATTATGATAAAAGATA+TGG - chr5_4:56223146-56223165 Msa0858630:intron 25.0%
! CAAAAAATGAAACAAGATGT+GGG - chr5_4:56223412-56223431 Msa0858630:CDS 25.0%
! TAAAAAAGATTTGTACAACC+AGG - chr5_4:56223680-56223699 Msa0858630:CDS 25.0%
! CAAAAACAATGGTTTGATTA+TGG + chr5_4:56223959-56223978 None:intergenic 25.0%
!!! CAATTTTTGATTGCAATGAA+CGG - chr5_4:56224032-56224051 Msa0858630:CDS 25.0%
! TTCAAATAAGACATGAAATC+AGG + chr5_4:56224191-56224210 None:intergenic 25.0%
! TTTGATTACCAATCCAATTA+TGG - chr5_4:56224503-56224522 Msa0858630:CDS 25.0%
! AATTGAATCTTTAGCAAACT+TGG + chr5_4:56224867-56224886 None:intergenic 25.0%
! TTTGCTAAAGATTCAATTTG+TGG - chr5_4:56224869-56224888 Msa0858630:CDS 25.0%
! ATTAATATGTGTTCATTAGG+TGG - chr5_4:56225367-56225386 Msa0858630:CDS 25.0%
! TTAATATGTGTTCATTAGGT+GGG - chr5_4:56225368-56225387 Msa0858630:CDS 25.0%
!!! TATTTAGTTTTTCCTCTTGT+AGG - chr5_4:56225398-56225417 Msa0858630:CDS 25.0%
TGATATAATGAAGATTGACC+TGG - chr5_4:56223170-56223189 Msa0858630:intron 30.0%
!!! CCACATCTTGTTTCATTTTT+TGG + chr5_4:56223414-56223433 None:intergenic 30.0%
CCAAAAAATGAAACAAGATG+TGG - chr5_4:56223411-56223430 Msa0858630:CDS 30.0%
AAACAATGATTTACGTTGCT+TGG + chr5_4:56223709-56223728 None:intergenic 30.0%
! CGTATGAACTATGAGATTTT+TGG - chr5_4:56223855-56223874 Msa0858630:CDS 30.0%
!! TGAGATTTTTGGAGATGTTT+TGG - chr5_4:56223866-56223885 Msa0858630:CDS 30.0%
! TAATGGCATTTTTCATTGCA+AGG + chr5_4:56224091-56224110 None:intergenic 30.0%
! TGCAGGAAAAACCTTTTTAA+TGG + chr5_4:56224108-56224127 None:intergenic 30.0%
! TCTGAAATGGTTCAAAAGTT+TGG - chr5_4:56224260-56224279 Msa0858630:CDS 30.0%
CTGAAATGGTTCAAAAGTTT+GGG - chr5_4:56224261-56224280 Msa0858630:CDS 30.0%
TGTATGAAAAGAAGAAGATG+TGG - chr5_4:56224312-56224331 Msa0858630:CDS 30.0%
GTATGAAAAGAAGAAGATGT+GGG - chr5_4:56224313-56224332 Msa0858630:CDS 30.0%
! ATTTTGTGCAACAATTTCAC+AGG - chr5_4:56224447-56224466 Msa0858630:CDS 30.0%
!!! GTCTATCATTTTTTCGATTC+CGG - chr5_4:56224471-56224490 Msa0858630:CDS 30.0%
!!! TCTATCATTTTTTCGATTCC+GGG - chr5_4:56224472-56224491 Msa0858630:CDS 30.0%
GGTAATCAAATAAAACTTCC+CGG + chr5_4:56224493-56224512 None:intergenic 30.0%
GTACGGAACATAAGAAATAT+TGG + chr5_4:56224593-56224612 None:intergenic 30.0%
TTCACGGTTTCAAAATATTG+TGG - chr5_4:56224677-56224696 Msa0858630:CDS 30.0%
GTTTCAAAATATTGTGGTGA+TGG - chr5_4:56224683-56224702 Msa0858630:CDS 30.0%
!! AAAATATTGTGGTGATGGTA+GGG - chr5_4:56224688-56224707 Msa0858630:CDS 30.0%
TAAATGTTCGTGTCTTAGAA+TGG - chr5_4:56224748-56224767 Msa0858630:CDS 30.0%
ATAGCAAGAATATGATCACA+TGG + chr5_4:56224788-56224807 None:intergenic 30.0%
TGAAGATCTTCCAAAATGTT+TGG - chr5_4:56224826-56224845 Msa0858630:CDS 30.0%
!! CAGGATTTTGAAGATGAAAG+TGG - chr5_4:56223027-56223046 Msa0858630:intron 35.0%
!! GATTTTGAAGATGAAAGTGG+AGG - chr5_4:56223030-56223049 Msa0858630:intron 35.0%
ACAAATTGCGAACAAAGCAA+TGG - chr5_4:56223063-56223082 Msa0858630:intron 35.0%
GGAAGTTCAAATTCAGTTGA+AGG - chr5_4:56223096-56223115 Msa0858630:intron 35.0%
TTCAAATTCAGTTGAAGGCT+CGG - chr5_4:56223101-56223120 Msa0858630:intron 35.0%
AAACCAAGAGTAGAACATGT+AGG + chr5_4:56223250-56223269 None:intergenic 35.0%
AACTGAAAATCCTGACATTC+TGG + chr5_4:56223274-56223293 None:intergenic 35.0%
! TCAGGATTTTCAGTTCGTAA+GGG - chr5_4:56223279-56223298 Msa0858630:CDS 35.0%
! ACCTTTGTTTGTTCATGTCA+AGG - chr5_4:56223342-56223361 Msa0858630:CDS 35.0%
ACCTTGACATGAACAAACAA+AGG + chr5_4:56223346-56223365 None:intergenic 35.0%
TTTGTTCATGTCAAGGTTAC+AGG - chr5_4:56223349-56223368 Msa0858630:CDS 35.0%
TTGTTCATGTCAAGGTTACA+GGG - chr5_4:56223350-56223369 Msa0858630:CDS 35.0%
! TCCACTTGGATATTTTGTCA+AGG - chr5_4:56223454-56223473 Msa0858630:CDS 35.0%
! TGGATATTTTGTCAAGGCAT+TGG - chr5_4:56223460-56223479 Msa0858630:CDS 35.0%
GATCACAATCATGCTATGTT+AGG - chr5_4:56223504-56223523 Msa0858630:CDS 35.0%
!! ACACTCATTTTTCTATACGC+TGG + chr5_4:56223552-56223571 None:intergenic 35.0%
ATAAGACCGTACCAAATGTA+TGG - chr5_4:56223612-56223631 Msa0858630:CDS 35.0%
TAAGACCGTACCAAATGTAT+GGG - chr5_4:56223613-56223632 Msa0858630:CDS 35.0%
! TCATTGTTTTCTGATGCTTG+TGG - chr5_4:56223720-56223739 Msa0858630:CDS 35.0%
TATACCAGTCGCAAAAACAA+TGG + chr5_4:56223970-56223989 None:intergenic 35.0%
CAACCATACGTATGTTTCTT+CGG + chr5_4:56224006-56224025 None:intergenic 35.0%
AGAAACATACGTATGGTTGT+TGG - chr5_4:56224007-56224026 Msa0858630:CDS 35.0%
TCTGTTATAACTGACAAAGG+AGG + chr5_4:56224062-56224081 None:intergenic 35.0%
CCATCTGTTATAACTGACAA+AGG + chr5_4:56224065-56224084 None:intergenic 35.0%
CCTTTGTCAGTTATAACAGA+TGG - chr5_4:56224062-56224081 Msa0858630:CDS 35.0%
TGAAGAACTTTGGTCTGAAA+TGG - chr5_4:56224247-56224266 Msa0858630:CDS 35.0%
GCGTTCATTAGAAAAATCTG+CGG - chr5_4:56224550-56224569 Msa0858630:CDS 35.0%
! ATGATCTTTTCAGCACTGTA+CGG + chr5_4:56224610-56224629 None:intergenic 35.0%
AGATAGCCAAGAAATGTCAA+TGG - chr5_4:56224646-56224665 Msa0858630:CDS 35.0%
GATAGCCAAGAAATGTCAAT+GGG - chr5_4:56224647-56224666 Msa0858630:CDS 35.0%
TATAGCCCATTGACATTTCT+TGG + chr5_4:56224655-56224674 None:intergenic 35.0%
!!! GTCAATGGGCTATATTTTCA+CGG - chr5_4:56224661-56224680 Msa0858630:CDS 35.0%
!! CAAAATATTGTGGTGATGGT+AGG - chr5_4:56224687-56224706 Msa0858630:CDS 35.0%
CACGAACATTTAAAGACAAC+TGG + chr5_4:56224740-56224759 None:intergenic 35.0%
! CTAGGCAAAACCAAACATTT+TGG + chr5_4:56224839-56224858 None:intergenic 35.0%
!!! CAAAATGTTTGGTTTTGCCT+AGG - chr5_4:56224837-56224856 Msa0858630:CDS 35.0%
ATTTGTGGTAGTTATGCCAA+TGG - chr5_4:56224884-56224903 Msa0858630:CDS 35.0%
ATGCCAATGGATCTCTATAT+TGG - chr5_4:56224897-56224916 Msa0858630:CDS 35.0%
TGCCAATGGATCTCTATATT+GGG - chr5_4:56224898-56224917 Msa0858630:CDS 35.0%
ATCCCAATATAGAGATCCAT+TGG + chr5_4:56224903-56224922 None:intergenic 35.0%
! AACCGTGTTGTTGATTATCT+GGG - chr5_4:56225007-56225026 Msa0858630:CDS 35.0%
TTGATCGGCTGAAATTGAAA+AGG - chr5_4:56225038-56225057 Msa0858630:CDS 35.0%
! TGACCTGGAAAACTTTTGTG+TGG - chr5_4:56223185-56223204 Msa0858630:intron 40.0%
! AAACTTTTGTGTGGCTGATG+TGG - chr5_4:56223194-56223213 Msa0858630:intron 40.0%
TTGCCTACATGTTCTACTCT+TGG - chr5_4:56223244-56223263 Msa0858630:CDS 40.0%
! GTCAGGATTTTCAGTTCGTA+AGG - chr5_4:56223278-56223297 Msa0858630:CDS 40.0%
! CAGGATTTTCAGTTCGTAAG+GGG - chr5_4:56223280-56223299 Msa0858630:CDS 40.0%
ATGAACAAACAAAGGTCTGC+TGG + chr5_4:56223338-56223357 None:intergenic 40.0%
!!! CATTTTTTGGCTCACGCTTT+CGG + chr5_4:56223401-56223420 None:intergenic 40.0%
! TGCAAAATTTCGAGTCCACT+TGG - chr5_4:56223440-56223459 Msa0858630:CDS 40.0%
GCCTTGACAAAATATCCAAG+TGG + chr5_4:56223458-56223477 None:intergenic 40.0%
CACAATCATGCTATGTTAGG+AGG - chr5_4:56223507-56223526 Msa0858630:CDS 40.0%
ACGCTCAATTTCAGCTATGT+CGG + chr5_4:56223577-56223596 None:intergenic 40.0%
AAGACCGTACCAAATGTATG+GGG - chr5_4:56223614-56223633 Msa0858630:CDS 40.0%
AAAGCCCCATACATTTGGTA+CGG + chr5_4:56223621-56223640 None:intergenic 40.0%
CAGCTTCTCACAAACATTAG+AGG + chr5_4:56223783-56223802 None:intergenic 40.0%
! GAAGCTGTATTGACGATGAT+AGG - chr5_4:56223796-56223815 Msa0858630:CDS 40.0%
ACACCTGAGAACACAACAAA+CGG + chr5_4:56223930-56223949 None:intergenic 40.0%
!!! CAAACCATTGTTTTTGCGAC+TGG - chr5_4:56223963-56223982 Msa0858630:CDS 40.0%
TTGCGACTGGTATAGTAACA+CGG - chr5_4:56223976-56223995 Msa0858630:CDS 40.0%
AGACCGAAGAAACATACGTA+TGG - chr5_4:56224000-56224019 Msa0858630:CDS 40.0%
AGGAATGCTTCAACCAACAT+TGG - chr5_4:56224161-56224180 Msa0858630:CDS 40.0%
GGAATGCTTCAACCAACATT+GGG - chr5_4:56224162-56224181 Msa0858630:CDS 40.0%
GAAATCAGGAATCCCAATGT+TGG + chr5_4:56224177-56224196 None:intergenic 40.0%
! TTGGGTTAGAAGACAACACT+TGG - chr5_4:56224279-56224298 Msa0858630:CDS 40.0%
ATCAGAGGAAGTTTCTTTGC+CGG - chr5_4:56224347-56224366 Msa0858630:CDS 40.0%
TGTCTTAGAATGGAATCCCT+TGG - chr5_4:56224758-56224777 Msa0858630:CDS 40.0%
GTCTTAGAATGGAATCCCTT+GGG - chr5_4:56224759-56224778 Msa0858630:CDS 40.0%
!!! AATGTTTGGTTTTGCCTAGG+TGG - chr5_4:56224840-56224859 Msa0858630:CDS 40.0%
! CAACCGTGTTGTTGATTATC+TGG - chr5_4:56225006-56225025 Msa0858630:CDS 40.0%
CACCCAGATAATCAACAACA+CGG + chr5_4:56225012-56225031 None:intergenic 40.0%
ATCTGGGTGGTGAAATTGAT+CGG - chr5_4:56225023-56225042 Msa0858630:CDS 40.0%
ACATTGATGCGAATCATGGA+GGG + chr5_4:56225244-56225263 None:intergenic 40.0%
AACATTGATGCGAATCATGG+AGG + chr5_4:56225245-56225264 None:intergenic 40.0%
CTGAACATTGATGCGAATCA+TGG + chr5_4:56225248-56225267 None:intergenic 40.0%
TTGTTACCCTCAGTTGACAT+CGG + chr5_4:56225283-56225302 None:intergenic 40.0%
AACGTTAACCGTCCATGTTA+TGG - chr5_4:56225441-56225460 Msa0858630:CDS 40.0%
TAACCGTCCATGTTATGGAA+TGG - chr5_4:56225446-56225465 Msa0858630:CDS 40.0%
CTTACCAGTAGTAATCTCGT+AGG + chr5_4:56225474-56225493 None:intergenic 40.0%
AAAACCCTGCAAAACCCAAA+AGG + chr5_4:56225594-56225613 None:intergenic 40.0%
AATCCTTTGTTAGAGCGCGA+TGG - chr5_4:56222907-56222926 Msa0858630:CDS 45.0%
CAAAGCAATGGAGATGTGAG+TGG - chr5_4:56223075-56223094 Msa0858630:intron 45.0%
AAATTCAGTTGAAGGCTCGG+AGG - chr5_4:56223104-56223123 Msa0858630:intron 45.0%
! CAGCCACACAAAAGTTTTCC+AGG + chr5_4:56223191-56223210 None:intergenic 45.0%
!! TCTTGGTTTGCCAGAATGTC+AGG - chr5_4:56223261-56223280 Msa0858630:CDS 45.0%
TTCATGTCAAGGTTACAGGG+AGG - chr5_4:56223353-56223372 Msa0858630:CDS 45.0%
TCAAGGTTACAGGGAGGATA+GGG - chr5_4:56223359-56223378 Msa0858630:CDS 45.0%
!! ATGTTAGGAGGTACTCTGTG+TGG - chr5_4:56223519-56223538 Msa0858630:CDS 45.0%
ATTGAGCGTAGTCGAAAAGC+TGG - chr5_4:56223588-56223607 Msa0858630:CDS 45.0%
GTACCAAATGTATGGGGCTT+TGG - chr5_4:56223620-56223639 Msa0858630:CDS 45.0%
!! TGCTTCTGGGGGTTTTCATA+AGG - chr5_4:56223647-56223666 Msa0858630:CDS 45.0%
! TCTGGGGGTTTTCATAAGGT+AGG - chr5_4:56223651-56223670 Msa0858630:CDS 45.0%
ATTTACGTTGCTTGGCAACC+TGG + chr5_4:56223701-56223720 None:intergenic 45.0%
! GCTGTATTGACGATGATAGG+AGG - chr5_4:56223799-56223818 Msa0858630:CDS 45.0%
!! TGTCCGTTTGTTGTGTTCTC+AGG - chr5_4:56223924-56223943 Msa0858630:CDS 45.0%
TGCGACTGGTATAGTAACAC+GGG - chr5_4:56223977-56223996 Msa0858630:CDS 45.0%
TGTGTGCTTGGCATCTGTTA+AGG - chr5_4:56224141-56224160 Msa0858630:CDS 45.0%
! ATGCATGTTGGCTGACATTG+AGG - chr5_4:56224214-56224233 Msa0858630:CDS 45.0%
ACGTGACGTGGTTCTTATAC+CGG + chr5_4:56224369-56224388 None:intergenic 45.0%
AATGCACGTATTGTCCCATG+TGG + chr5_4:56224409-56224428 None:intergenic 45.0%
TGGGACAATACGTGCATTCT+AGG - chr5_4:56224411-56224430 Msa0858630:CDS 45.0%
CAGGCTGACCATAATTGGAT+TGG + chr5_4:56224514-56224533 None:intergenic 45.0%
CATCACAGGCTGACCATAAT+TGG + chr5_4:56224519-56224538 None:intergenic 45.0%
!! ATTGTGGTGATGGTAGGGTT+TGG - chr5_4:56224693-56224712 Msa0858630:CDS 45.0%
ATGATCACATGGCAACCCAA+GGG + chr5_4:56224777-56224796 None:intergenic 45.0%
TATGATCACATGGCAACCCA+AGG + chr5_4:56224778-56224797 None:intergenic 45.0%
TTAGCAAACTTGGACCACCT+AGG + chr5_4:56224857-56224876 None:intergenic 45.0%
! CAAATTGCCGCAAGGTATTC+AGG - chr5_4:56224926-56224945 Msa0858630:CDS 45.0%
GACTATGCCTGAATACCTTG+CGG + chr5_4:56224936-56224955 None:intergenic 45.0%
AGTCCAGATGTGTAAAGTCG+TGG - chr5_4:56224952-56224971 Msa0858630:CDS 45.0%
! CGTGTTGTTGATTATCTGGG+TGG - chr5_4:56225010-56225029 Msa0858630:CDS 45.0%
GAACTGTGATTCAGTTCCAG+TGG - chr5_4:56225069-56225088 Msa0858630:CDS 45.0%
CCTTTGTCCTTACACACTGT+GGG + chr5_4:56225126-56225145 None:intergenic 45.0%
CCACAGTGTGTAAGGACAAA+GGG - chr5_4:56225124-56225143 Msa0858630:CDS 45.0%
TGTGTAAGGACAAAGGGTTG+TGG - chr5_4:56225130-56225149 Msa0858630:CDS 45.0%
GACGGTTAACGTTTCAATCC+TGG + chr5_4:56225434-56225453 None:intergenic 45.0%
GCACCATTCCATAACATGGA+CGG + chr5_4:56225452-56225471 None:intergenic 45.0%
GTAGGCACCATTCCATAACA+TGG + chr5_4:56225456-56225475 None:intergenic 45.0%
CCATCTGCAACACATGAAGT+TGG + chr5_4:56225553-56225572 None:intergenic 45.0%
CCAACTTCATGTGTTGCAGA+TGG - chr5_4:56225550-56225569 Msa0858630:intron 45.0%
!! TTGCAGATGGATTGCAGTGA+TGG - chr5_4:56225563-56225582 Msa0858630:CDS 45.0%
!!! CAGTGATGGAAGTGCCTTTT+GGG - chr5_4:56225577-56225596 Msa0858630:CDS 45.0%
!!! AAGTGCCTTTTGGGTTTTGC+AGG - chr5_4:56225586-56225605 Msa0858630:CDS 45.0%
!!! AGTGCCTTTTGGGTTTTGCA+GGG - chr5_4:56225587-56225606 Msa0858630:CDS 45.0%
ACACCATCGCGCTCTAACAA+AGG + chr5_4:56222913-56222932 None:intergenic 50.0%
TTCAGTTCGTAAGGGGCAAG+TGG - chr5_4:56223287-56223306 Msa0858630:CDS 50.0%
CAAGTGGTGAGGGATCGAAA+TGG - chr5_4:56223303-56223322 Msa0858630:CDS 50.0%
GTCAAGGTTACAGGGAGGAT+AGG - chr5_4:56223358-56223377 Msa0858630:CDS 50.0%
CAAGGTTACAGGGAGGATAG+GGG - chr5_4:56223360-56223379 Msa0858630:CDS 50.0%
AAGGTTACAGGGAGGATAGG+GGG - chr5_4:56223361-56223380 Msa0858630:CDS 50.0%
GGAGGATAGGGGGTTAACAA+TGG - chr5_4:56223371-56223390 Msa0858630:CDS 50.0%
CTGCCAAAGCCCCATACATT+TGG + chr5_4:56223626-56223645 None:intergenic 50.0%
ACGATGATAGGAGGCTTGAG+CGG - chr5_4:56223808-56223827 Msa0858630:CDS 50.0%
TGGCCTTCGATGCAACGTAT+CGG - chr5_4:56223886-56223905 Msa0858630:CDS 50.0%
CTTCCGATACGTTGCATCGA+AGG + chr5_4:56223892-56223911 None:intergenic 50.0%
! TTTTTCCTGCAGCACACCAC+AGG - chr5_4:56224117-56224136 Msa0858630:CDS 50.0%
ACTTCCTCTGATGTGAGCAG+TGG + chr5_4:56224339-56224358 None:intergenic 50.0%
AAGAACCACGTCACGTTGTG+AGG - chr5_4:56224373-56224392 Msa0858630:CDS 50.0%
GGGATTCACAAATTGCCGCA+AGG - chr5_4:56224918-56224937 Msa0858630:CDS 50.0%
CAGCCACGACTTTACACATC+TGG + chr5_4:56224958-56224977 None:intergenic 50.0%
GTGTAAAGTCGTGGCTGAGA+TGG - chr5_4:56224961-56224980 Msa0858630:CDS 50.0%
TCTTGGACCCACAGTGTGTA+AGG - chr5_4:56225116-56225135 Msa0858630:CDS 50.0%
CCCACAGTGTGTAAGGACAA+AGG - chr5_4:56225123-56225142 Msa0858630:CDS 50.0%
CCCTTTGTCCTTACACACTG+TGG + chr5_4:56225127-56225146 None:intergenic 50.0%
TGAGTACGTTTGCGTCTACC+CGG + chr5_4:56225175-56225194 None:intergenic 50.0%
ACTCAGACTTGCAGCTTGTG+CGG - chr5_4:56225190-56225209 Msa0858630:CDS 50.0%
TGCAGCTTGTGCGGTGTAAT+AGG - chr5_4:56225199-56225218 Msa0858630:CDS 50.0%
ACAGTCACCGATGTCAACTG+AGG - chr5_4:56225273-56225292 Msa0858630:CDS 50.0%
CAGTCACCGATGTCAACTGA+GGG - chr5_4:56225274-56225293 Msa0858630:CDS 50.0%
GGCTCAACATGTCCTACAAG+AGG + chr5_4:56225413-56225432 None:intergenic 50.0%
CTTGTAGGACATGTTGAGCC+AGG - chr5_4:56225413-56225432 Msa0858630:CDS 50.0%
GGTGCCTACGAGATTACTAC+TGG - chr5_4:56225467-56225486 Msa0858630:intron 50.0%
!!! GCAGTGATGGAAGTGCCTTT+TGG - chr5_4:56225576-56225595 Msa0858630:CDS 50.0%
TTCGTAAGGGGCAAGTGGTG+AGG - chr5_4:56223292-56223311 Msa0858630:CDS 55.0%
TCGTAAGGGGCAAGTGGTGA+GGG - chr5_4:56223293-56223312 Msa0858630:CDS 55.0%
! GGGCTTTGGCAGATGCTTCT+GGG - chr5_4:56223634-56223653 Msa0858630:CDS 55.0%
! GGCTTTGGCAGATGCTTCTG+GGG - chr5_4:56223635-56223654 Msa0858630:CDS 55.0%
GCTTTGGCAGATGCTTCTGG+GGG - chr5_4:56223636-56223655 Msa0858630:CDS 55.0%
CACACCACAGGTTGTGTGCT+TGG - chr5_4:56224129-56224148 Msa0858630:CDS 55.0%
GATGCCAAGCACACAACCTG+TGG + chr5_4:56224136-56224155 None:intergenic 55.0%
CGTATTGTCCCATGTGGCTG+TGG + chr5_4:56224403-56224422 None:intergenic 55.0%
CGCAAACCAGTCTGCATCAC+AGG + chr5_4:56224533-56224552 None:intergenic 55.0%
TCCAGTGGTGCAGAACAGCA+TGG - chr5_4:56225084-56225103 Msa0858630:CDS 55.0%
TCCATGCTGTTCTGCACCAC+TGG + chr5_4:56225088-56225107 None:intergenic 55.0%
CAGCATGGACGAGGATGTCT+TGG - chr5_4:56225099-56225118 Msa0858630:CDS 55.0%
GGTTGTGGTGCTTACCCAAG+CGG - chr5_4:56225145-56225164 Msa0858630:CDS 55.0%
TGCTTACCCAAGCGGTACTC+CGG - chr5_4:56225153-56225172 Msa0858630:CDS 55.0%
! GGGGCTTTGGCAGATGCTTC+TGG - chr5_4:56223633-56223652 Msa0858630:CDS 60.0%
GAGCGGCTGTTCTGGTGTGA+CGG - chr5_4:56223825-56223844 Msa0858630:CDS 60.0%
CACAACCTGTGGTGTGCTGC+AGG + chr5_4:56224125-56224144 None:intergenic 60.0%
TGGGCCACTGCTCACATCAG+AGG - chr5_4:56224332-56224351 Msa0858630:CDS 60.0%
GAGTGCCTCACAACGTGACG+TGG + chr5_4:56224381-56224400 None:intergenic 60.0%
! TGAGGCACTCCACAGCCACA+TGG - chr5_4:56224391-56224410 Msa0858630:CDS 60.0%
! GAGGCACTCCACAGCCACAT+GGG - chr5_4:56224392-56224411 Msa0858630:CDS 60.0%
GGTCAGCCTGTGATGCAGAC+TGG - chr5_4:56224524-56224543 Msa0858630:CDS 60.0%
CGTGGCTGAGATGGTCTACG+AGG - chr5_4:56224970-56224989 Msa0858630:CDS 60.0%
GGTGCAGAACAGCATGGACG+AGG - chr5_4:56225090-56225109 Msa0858630:CDS 60.0%
GCTTACCCAAGCGGTACTCC+GGG - chr5_4:56225154-56225173 Msa0858630:CDS 60.0%
!! GTCTACCCGGAGTACCGCTT+GGG + chr5_4:56225162-56225181 None:intergenic 60.0%
GGAGGCTTGAGCGGCTGTTC+TGG - chr5_4:56223817-56223836 Msa0858630:CDS 65.0%
!! CGTCTACCCGGAGTACCGCT+TGG + chr5_4:56225163-56225182 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 56222883 56225619 56222883 ID=Msa0858630;Name=Msa0858630
chr5_4 mRNA 56222883 56225619 56222883 ID=Msa0858630-mRNA-1;Parent=Msa0858630;Name=Msa0858630-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0.5|0|0.66|1|1|3|0|795
chr5_4 exon 56225557 56225619 56225557 ID=Msa0858630-mRNA-1:exon:17205;Parent=Msa0858630-mRNA-1
chr5_4 exon 56223199 56225472 56223199 ID=Msa0858630-mRNA-1:exon:17204;Parent=Msa0858630-mRNA-1
chr5_4 exon 56222883 56222933 56222883 ID=Msa0858630-mRNA-1:exon:17203;Parent=Msa0858630-mRNA-1
chr5_4 CDS 56225557 56225619 56225557 ID=Msa0858630-mRNA-1:cds;Parent=Msa0858630-mRNA-1
chr5_4 CDS 56223199 56225472 56223199 ID=Msa0858630-mRNA-1:cds;Parent=Msa0858630-mRNA-1
chr5_4 CDS 56222883 56222933 56222883 ID=Msa0858630-mRNA-1:cds;Parent=Msa0858630-mRNA-1
Gene Sequence

>Msa0858630

ATGGACGGACGTTCAGAGGCTGTGAATCCTTTGTTAGAGCGCGATGGTGTGAGAATTTCAAGAGATTTTGAAGATGAAAGTGGAGGTAATAACAACACAAATTGCGAACAAAGCAATGGAGATGTGAGTGGAAGTTCAAATTCAGTTGAAGGCTCGGAGGATGAAACTAATGAAGTCAATCCGATTATGATAAAAGATATGGTTGATATAATGAAGATTGACCTGGAAAACTTTTGTGTGGCTGATGTGGAAAGATATGAATTTGTTGCTTTAGATGTTGCCTACATGTTCTACTCTTGGTTTGCCAGAATGTCAGGATTTTCAGTTCGTAAGGGGCAAGTGGTGAGGGATCGAAATGGTGAAGTCGTCCAGCAGACCTTTGTTTGTTCATGTCAAGGTTACAGGGAGGATAGGGGGTTAACAATGGAAAACCGAAAGCGTGAGCCAAAAAATGAAACAAGATGTGGGTGTAATGCAAAATTTCGAGTCCACTTGGATATTTTGTCAAGGCATTGGTATGTTACTGTATTTGAATTTGATCACAATCATGCTATGTTAGGAGGTACTCTGTGTGGAATGTTGCCAGCGTATAGAAAAATGAGTGTAGCCGACATAGCTGAAATTGAGCGTAGTCGAAAAGCTGGTATAAGACCGTACCAAATGTATGGGGCTTTGGCAGATGCTTCTGGGGGTTTTCATAAGGTAGGATTTGTTAAAAAAGATTTGTACAACCAGGTTGCCAAGCAACGTAAATCATTGTTTTCTGATGCTTGTGGCGCAGTTAAGTATCTTCGAGATCTTTGCAAAGACGATCCTCTAATGTTTGTGAGAAGCTGTATTGACGATGATAGGAGGCTTGAGCGGCTGTTCTGGTGTGACGGTGAAAGTCGTATGAACTATGAGATTTTTGGAGATGTTTTGGCCTTCGATGCAACGTATCGGAAGAACAAGTACAACTGTCCGTTTGTTGTGTTCTCAGGTGTTAATCACCATAATCAAACCATTGTTTTTGCGACTGGTATAGTAACACGGGAGACCGAAGAAACATACGTATGGTTGTTGGAACAATTTTTGATTGCAATGAACGGTAAACCTCCTTTGTCAGTTATAACAGATGGCGACCTTGCAATGAAAAATGCCATTAAAAAGGTTTTTCCTGCAGCACACCACAGGTTGTGTGCTTGGCATCTGTTAAGGAATGCTTCAACCAACATTGGGATTCCTGATTTCATGTCTTATTTGAAAAAATGCATGTTGGCTGACATTGAGGTTAACAAATTTGAAGAACTTTGGTCTGAAATGGTTCAAAAGTTTGGGTTAGAAGACAACACTTGGATCAATGAGATGTATGAAAAGAAGAAGATGTGGGCCACTGCTCACATCAGAGGAAGTTTCTTTGCCGGTATAAGAACCACGTCACGTTGTGAGGCACTCCACAGCCACATGGGACAATACGTGCATTCTAGGATAAACATGACTGATTTTGTGCAACAATTTCACAGGTGTCTATCATTTTTTCGATTCCGGGAAGTTTTATTTGATTACCAATCCAATTATGGTCAGCCTGTGATGCAGACTGGTTTGCGTTCATTAGAAAAATCTGCGGCTAATCAGTTCACAAAGCCAATATTTCTTATGTTCCGTACAGTGCTGAAAAGATCATTGCTTCTTAGAATTACAGATAGCCAAGAAATGTCAATGGGCTATATTTTCACGGTTTCAAAATATTGTGGTGATGGTAGGGTTTGGCACGTAACATACTGTGAAGAACCAGTTGTCTTTAAATGTTCGTGTCTTAGAATGGAATCCCTTGGGTTGCCATGTGATCATATTCTTGCTATTCTCTTGTATCTTGATTTTGAAGATCTTCCAAAATGTTTGGTTTTGCCTAGGTGGTCCAAGTTTGCTAAAGATTCAATTTGTGGTAGTTATGCCAATGGATCTCTATATTGGGATTCACAAATTGCCGCAAGGTATTCAGGCATAGTCCAGATGTGTAAAGTCGTGGCTGAGATGGTCTACGAGGATGACGATGAGTACAACCGTGTTGTTGATTATCTGGGTGGTGAAATTGATCGGCTGAAATTGAAAAGGAACAATGAGAACTGTGATTCAGTTCCAGTGGTGCAGAACAGCATGGACGAGGATGTCTTGGACCCACAGTGTGTAAGGACAAAGGGTTGTGGTGCTTACCCAAGCGGTACTCCGGGTAGACGCAAACGTACTCAGACTTGCAGCTTGTGCGGTGTAATAGGTCATAACAAACGTTGCTGTCCCTCCATGATTCGCATCAATGTTCAGCAGACACAGTCACCGATGTCAACTGAGGGTAACAATATGGATTGCAGTGATGGAAGTGCCTTTTGGGTTTTGCAGGGTTTTAATTAA
Protein sequence

>Msa0858630

MDGRSEAVNPLLERDGVRISRDFEDESGGNNNTNCEQSNGDVSGSSNSVEGSEDETNEVNPIMIKDMVDIMKIDLENFCVADVERYEFVALDVAYMFYSWFARMSGFSVRKGQVVRDRNGEVVQQTFVCSCQGYREDRGLTMENRKREPKNETRCGCNAKFRVHLDILSRHWYVTVFEFDHNHAMLGGTLCGMLPAYRKMSVADIAEIERSRKAGIRPYQMYGALADASGGFHKVGFVKKDLYNQVAKQRKSLFSDACGAVKYLRDLCKDDPLMFVRSCIDDDRRLERLFWCDGESRMNYEIFGDVLAFDATYRKNKYNCPFVVFSGVNHHNQTIVFATGIVTRETEETYVWLLEQFLIAMNGKPPLSVITDGDLAMKNAIKKVFPAAHHRLCAWHLLRNASTNIGIPDFMSYLKKCMLADIEVNKFEELWSEMVQKFGLEDNTWINEMYEKKKMWATAHIRGSFFAGIRTTSRCEALHSHMGQYVHSRINMTDFVQQFHRCLSFFRFREVLFDYQSNYGQPVMQTGLRSLEKSAANQFTKPIFLMFRTVLKRSLLLRITDSQEMSMGYIFTVSKYCGDGRVWHVTYCEEPVVFKCSCLRMESLGLPCDHILAILLYLDFEDLPKCLVLPRWSKFAKDSICGSYANGSLYWDSQIAARYSGIVQMCKVVAEMVYEDDDEYNRVVDYLGGEIDRLKLKRNNENCDSVPVVQNSMDEDVLDPQCVRTKGCGAYPSGTPGRRKRTQTCSLCGVIGHNKRCCPSMIRINVQQTQSPMSTEGNNMDCSDGSAFWVLQGFN*