AlfalfaGEDB | Msa0861230

M. sativa cultivar zhongmu-4 / Msa0861230

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0861230	AES92029.1	80.311	193	17	2	29	200	31	223	3.36e-107	317

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0861230	G7JQ00	80.311	193	17	2	29	200	31	223	1.60e-107	317

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TFs/TRs:

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Gene ID	Type	Classification
Msa0861230	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0861230	MtrunA17_Chr5g0393501	32.308	195	108	4	29	199	45	239	8.37e-28	105
Msa0861230	MtrunA17_Chr4g0044671	27.222	180	110	2	29	187	28	207	4.26e-14	68.2
Msa0861230	MtrunA17_Chr4g0034781	26.667	180	111	2	29	187	22	201	1.53e-13	66.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 53 sgRNAs with CRISPR-Local

Find 317 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCGTGATGTTTCACTCTA+AGG	0.341127	5_4:-60488886	None:intergenic
ACACGATGCTGCCGCACTCA+TGG	0.487735	5_4:-60488685	None:intergenic
ACCCTATCATTCCATGAGTG+CGG	0.640367	5_4:+60488674	Msa0861230:CDS
ACGGAAATGATATTGTCATA+AGG	0.522697	5_4:-60488926	None:intergenic
AGATTTGCGATTTCCAATTT+GGG	0.327058	5_4:+60486712	Msa0861230:CDS
AGCCGTTGTTGACAGATGGT+TGG	0.550734	5_4:+60488532	Msa0861230:CDS
AGGAATTTGCCGAAAGTATC+AGG	0.335653	5_4:-60488866	None:intergenic
AGTCAGCCGTTGTTGACAGA+TGG	0.548377	5_4:+60488528	Msa0861230:CDS
ATCTACTTCCACCAAGGTAC+TGG	0.428620	5_4:-60488605	None:intergenic
ATGTGATGTTCGGAAAAGGT+TGG	0.606400	5_4:+60488981	Msa0861230:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATTTGCGATTTCCAATTT+GGG	+	chr5_4:60486712-60486731	Msa0861230:CDS	30.0%
TGGTCCCTTAAGTATTTATT+TGG	+	chr5_4:60486822-60486841	Msa0861230:intron	30.0%
ATTTCTCTTTCCTCTTACTT+CGG	-	chr5_4:60487113-60487132	None:intergenic	30.0%
AGAAAATCTAGAGAGTCATA+GGG	-	chr5_4:60487474-60487493	None:intergenic	30.0%
TATGCTATGAAAAATAGGGA+TGG	-	chr5_4:60487585-60487604	None:intergenic	30.0%
AATCATTGGGAATAGGAAAA+TGG	+	chr5_4:60487694-60487713	Msa0861230:intron	30.0%
TCATTAGTACATGTTTAGGA+GGG	-	chr5_4:60487814-60487833	None:intergenic	30.0%
TTGATTAGCAATTAGTGTAG+GGG	-	chr5_4:60487975-60487994	None:intergenic	30.0%
ATCTTCCTATCACTCTATTT+CGG	-	chr5_4:60488141-60488160	None:intergenic	30.0%
ATAGGAAGATATGCGTTTAA+AGG	+	chr5_4:60488151-60488170	Msa0861230:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	60486665	60489096	60486665	ID=Msa0861230;Name=Msa0861230
chr5_4	mRNA	60486665	60489096	60486665	ID=Msa0861230-mRNA-1;Parent=Msa0861230;Name=Msa0861230-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0\|0\|0\|0.75\|0.66\|0\|4\|0\|200
chr5_4	exon	60486665	60486749	60486665	ID=Msa0861230-mRNA-1:exon:18673;Parent=Msa0861230-mRNA-1
chr5_4	exon	60488421	60488562	60488421	ID=Msa0861230-mRNA-1:exon:18674;Parent=Msa0861230-mRNA-1
chr5_4	exon	60488587	60488722	60488587	ID=Msa0861230-mRNA-1:exon:18675;Parent=Msa0861230-mRNA-1
chr5_4	exon	60488860	60489096	60488860	ID=Msa0861230-mRNA-1:exon:18676;Parent=Msa0861230-mRNA-1
chr5_4	CDS	60486665	60486749	60486665	ID=Msa0861230-mRNA-1:cds;Parent=Msa0861230-mRNA-1
chr5_4	CDS	60488421	60488562	60488421	ID=Msa0861230-mRNA-1:cds;Parent=Msa0861230-mRNA-1
chr5_4	CDS	60488587	60488722	60488587	ID=Msa0861230-mRNA-1:cds;Parent=Msa0861230-mRNA-1
chr5_4	CDS	60488860	60489096	60488860	ID=Msa0861230-mRNA-1:cds;Parent=Msa0861230-mRNA-1

Gene Sequence

>Msa0861230

ATTTGGGTAAGGGGTTTGCGATTTGGGGAAGAGGTGGAGAAGGTTCCAGATTTGCGATTTCCAATTTGGGGAAGAGGAGGAGAAGAACTACAACAATTATTCGTTGCTAAATATTTGTACGAGCTACCGGAGCAATGGCACTTGCTAGATGGTGATGGGAATACTCATAACGTCATGTTCAACAAATCAGTTAGTCAGCCGTTGTTGACAGATGGTTGGAGTGCATTAAGTAAGATTATTGCTTTCCAGTACCTTGGTGGAAGTAGATTTCAAATCTTTGTTTTTGCTCATAACATTGCTCCAGCTGATTATCCACCCTATCATTCCATGAGTGCGGCAGCATCGTGTTATTGTACCTTTGAAGAACTACCTGATACTTTCGGCAAATTCCTTAGAGTGAAACATCACGATTACGTCATGTTGTGCAGACCTTATGACAATATCATTTCCGTGAGACTCAAATATACAGACGACCCCATACCAAATGTGATGTTCGGAAAAGGTTGGACGAGTTTTTGTGCTGCCAATGGTATTGAGGGCGGAGACGTGTTGATTTTCAAGTGCAAGTGCGTCATGGACAAAAAATATGTTATCGTATCC

Protein sequence

>Msa0861230

IWVRGLRFGEEVEKVPDLRFPIWGRGGEELQQLFVAKYLYELPEQWHLLDGDGNTHNVMFNKSVSQPLLTDGWSALSKIIAFQYLGGSRFQIFVFAHNIAPADYPPYHSMSAAASCYCTFEELPDTFGKFLRVKHHDYVMLCRPYDNIISVRLKYTDDPIPNVMFGKGWTSFCAANGIEGGDVLIFKCKCVMDKKYVIVS