AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0861260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0861260 MtrunA17_Chr3g0082121 76.157 281 64 2 208 485 412 692 2.12e-140 422
Msa0861260 MtrunA17_Chr3g0082121 61.979 192 71 2 15 205 2 192 2.27e-69 236
Msa0861260 MtrunA17_Chr5g0401121 65.116 129 41 1 33 161 36 160 2.83e-48 165
Msa0861260 MtrunA17_Chr2g0324011 44.949 198 105 1 208 401 386 583 9.18e-48 175
Msa0861260 MtrunA17_Chr2g0324011 45.638 149 76 4 57 203 10 155 9.06e-28 117
Msa0861260 MtrunA17_Chr1g0203721 40.244 246 138 4 208 445 442 686 5.67e-43 164
Msa0861260 MtrunA17_Chr1g0203721 38.843 121 72 2 48 167 54 173 3.03e-20 95.1
Msa0861260 MtrunA17_Chr6g0458391 40.761 184 104 3 208 387 584 766 4.11e-35 140
Msa0861260 MtrunA17_Chr6g0458391 42.331 163 79 5 21 178 194 346 4.67e-30 125
Msa0861260 MtrunA17_Chr6g0458391 32.710 214 127 8 57 268 91 289 7.73e-22 99.8
Msa0861260 MtrunA17_Chr5g0448761 30.769 286 185 8 173 452 413 691 1.53e-28 120
Msa0861260 MtrunA17_Chr5g0448761 37.126 167 96 4 48 205 46 212 2.24e-23 104
Msa0861260 MtrunA17_Chr7g0258861 44.667 150 77 5 57 203 10 156 3.67e-28 114
Msa0861260 MtrunA17_Chr3g0118611 31.229 301 182 10 158 448 276 561 1.23e-26 114
Msa0861260 MtrunA17_Chr2g0287651 28.723 282 169 8 210 479 451 712 8.03e-25 109
Msa0861260 MtrunA17_Chr2g0287651 31.892 185 101 7 40 204 45 224 1.79e-13 73.6
Msa0861260 MtrunA17_Chr1g0207551 30.734 218 139 4 208 420 408 618 9.50e-24 105
Msa0861260 MtrunA17_Chr8g0363841 32.609 184 118 2 209 386 394 577 1.27e-23 105
Msa0861260 MtrunA17_Chr1g0164591 65.278 72 24 1 135 205 1 72 1.15e-22 99.4
Msa0861260 MtrunA17_Chr5g0448511 25.080 311 218 6 208 513 215 515 1.25e-22 101
Msa0861260 MtrunA17_Chr1g0202341 32.787 183 117 2 209 386 428 609 2.42e-22 101
Msa0861260 MtrunA17_Chr1g0202341 29.016 193 125 6 21 205 10 198 4.49e-13 72.0
Msa0861260 MtrunA17_Chr5g0441581 28.679 265 163 9 209 455 397 653 7.10e-22 99.8
Msa0861260 MtrunA17_Chr5g0440451 30.488 246 161 5 208 448 424 664 2.60e-21 98.6
Msa0861260 MtrunA17_Chr2g0329031 27.119 295 190 12 210 488 383 668 5.29e-21 97.1
Msa0861260 MtrunA17_Chr2g0329031 35.246 122 73 3 52 171 7 124 4.15e-15 78.6
Msa0861260 MtrunA17_Chr3g0080351 26.556 241 161 4 210 443 444 675 6.48e-20 94.0
Msa0861260 MtrunA17_Chr6g0477261 28.767 219 144 6 179 386 228 445 1.93e-17 85.9
Msa0861260 MtrunA17_Chr1g0198761 29.609 179 124 2 210 387 436 613 4.58e-17 84.7
Msa0861260 MtrunA17_Chr2g0276841 32.184 174 117 1 210 383 454 626 5.98e-17 84.3
Msa0861260 MtrunA17_Chr2g0276841 31.915 141 91 4 54 189 67 207 4.39e-13 72.0
Msa0861260 MtrunA17_Chr7g0233911 34.507 142 78 4 25 159 3 136 6.21e-17 80.1
Msa0861260 MtrunA17_Chr2g0296781 30.570 193 122 6 205 386 365 556 6.47e-17 84.3
Msa0861260 MtrunA17_Chr7g0238821 42.202 109 58 4 61 167 1 106 6.66e-17 76.6
Msa0861260 MtrunA17_Chr2g0291581 30.102 196 110 6 210 386 434 621 7.65e-17 84.0
Msa0861260 MtrunA17_Chr4g0033611 42.056 107 56 3 57 161 76 178 2.65e-16 79.0
Msa0861260 MtrunA17_Chr2g0329421 40.187 107 58 3 57 161 27 129 4.59e-16 77.4
Msa0861260 MtrunA17_Chr2g0290391 26.136 264 177 7 210 469 443 692 1.85e-15 79.7
Msa0861260 MtrunA17_Chr2g0290391 30.216 139 88 5 57 189 51 186 9.98e-12 67.8
Msa0861260 MtrunA17_Chr2g0287061 28.497 193 120 5 210 392 523 707 2.90e-15 79.3
Msa0861260 MtrunA17_Chr3g0078041 38.462 104 62 2 57 159 50 152 5.89e-14 71.6
Msa0861260 MtrunA17_Chr2g0294841 28.191 188 130 4 210 395 432 616 1.03e-13 74.3
Msa0861260 MtrunA17_Chr2g0294841 30.726 179 107 8 37 203 26 199 5.53e-12 68.6
Msa0861260 MtrunA17_Chr2g0308841 23.656 186 133 5 210 388 46 229 6.69e-13 69.3
Msa0861260 MtrunA17_Chr2g0329361 36.190 105 64 2 57 159 61 164 5.23e-12 66.2
Msa0861260 MtrunA17_Chr1g0196541 29.348 184 118 5 209 381 369 551 6.72e-12 68.6
Msa0861260 MtrunA17_Chr1g0203051 29.677 155 89 6 63 206 85 230 2.60e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0861260 AT3G22170.2 31.624 234 137 6 208 431 437 657 4.32e-27 116
Msa0861260 AT3G22170.1 31.624 234 137 6 208 431 437 657 4.32e-27 116
Msa0861260 AT4G38180.1 30.469 256 170 5 195 445 441 693 2.76e-26 114
Msa0861260 AT4G38180.1 36.813 182 104 5 32 205 46 224 7.69e-22 100
Msa0861260 AT4G15090.1 28.980 245 164 3 208 448 428 666 2.73e-25 111
Msa0861260 AT4G15090.5 28.980 245 164 3 208 448 428 666 2.73e-25 111
Msa0861260 AT4G15090.3 28.980 245 164 3 208 448 428 666 2.73e-25 111
Msa0861260 AT4G15090.2 28.980 245 164 3 208 448 428 666 2.73e-25 111
Msa0861260 AT4G15090.4 28.980 245 164 3 208 448 428 666 2.73e-25 111
Msa0861260 AT2G27110.2 32.922 243 149 8 209 444 400 635 4.14e-25 110
Msa0861260 AT2G27110.1 32.922 243 149 8 209 444 400 635 4.14e-25 110
Msa0861260 AT2G27110.3 32.922 243 149 8 209 444 255 490 5.45e-25 110
Msa0861260 AT1G76320.1 29.825 228 144 8 210 431 367 584 4.17e-22 101
Msa0861260 AT1G76320.1 33.333 132 78 3 61 192 1 122 8.45e-14 74.7
Msa0861260 AT1G76320.4 29.825 228 144 8 210 431 367 584 4.17e-22 101
Msa0861260 AT1G76320.4 33.333 132 78 3 61 192 1 122 8.45e-14 74.7
Msa0861260 AT1G76320.2 29.825 228 144 8 210 431 367 584 4.23e-22 100
Msa0861260 AT1G76320.2 33.333 132 78 3 61 192 1 122 8.66e-14 74.7
Msa0861260 AT1G76320.3 29.825 228 144 8 210 431 367 584 6.65e-22 100
Msa0861260 AT1G76320.3 33.333 132 78 3 61 192 1 122 8.97e-14 74.7
Msa0861260 AT5G18960.2 28.395 243 162 6 206 443 547 782 9.85e-20 93.6
Msa0861260 AT5G18960.1 28.395 243 162 6 206 443 547 782 9.85e-20 93.6
Msa0861260 AT4G19990.3 25.882 340 215 7 130 441 280 610 1.21e-19 93.6
Msa0861260 AT4G19990.4 25.882 340 215 7 130 441 280 610 1.21e-19 93.6
Msa0861260 AT4G19990.5 25.882 340 215 7 130 441 280 610 1.23e-19 93.6
Msa0861260 AT4G19990.6 25.882 340 215 7 130 441 280 610 1.24e-19 93.6
Msa0861260 AT4G19990.2 25.882 340 215 7 130 441 280 610 1.24e-19 93.6
Msa0861260 AT4G19990.1 25.882 340 215 7 130 441 223 553 1.32e-19 93.2
Msa0861260 AT4G19990.7 25.882 340 215 7 130 441 294 624 1.48e-19 93.2
Msa0861260 AT3G06250.2 28.194 227 154 5 208 429 525 747 4.56e-19 91.7
Msa0861260 AT3G06250.1 28.194 227 154 5 208 429 525 747 4.56e-19 91.7
Msa0861260 AT3G06250.3 28.194 227 154 5 208 429 525 747 4.56e-19 91.7
Msa0861260 AT4G38170.1 27.027 185 129 3 208 387 217 400 5.05e-19 90.9
Msa0861260 AT2G32250.2 27.083 240 165 4 208 443 372 605 3.54e-17 85.9
Msa0861260 AT2G32250.4 27.083 240 165 4 208 443 372 605 3.54e-17 85.9
Msa0861260 AT2G32250.6 27.083 240 165 4 208 443 372 605 3.68e-17 85.5
Msa0861260 AT2G32250.1 27.083 240 165 4 208 443 372 605 3.68e-17 85.5
Msa0861260 AT2G32250.3 27.083 240 165 4 208 443 372 605 3.68e-17 85.5
Msa0861260 AT2G32250.7 26.199 271 189 5 208 473 331 595 3.93e-17 85.5
Msa0861260 AT2G32250.8 26.199 271 189 5 208 473 331 595 3.96e-17 85.5
Msa0861260 AT2G32250.5 27.083 240 165 4 208 443 372 605 4.39e-17 85.5
Msa0861260 AT3G59470.3 35.762 151 85 3 24 164 27 175 2.47e-16 79.3
Msa0861260 AT3G59470.1 35.762 151 85 3 24 164 27 175 2.47e-16 79.3
Msa0861260 AT3G59470.2 35.762 151 85 3 24 164 39 187 3.46e-16 79.3
Msa0861260 AT4G12850.4 36.364 132 78 4 50 181 4 129 1.61e-15 74.3
Msa0861260 AT4G12850.1 38.739 111 64 3 50 160 4 110 9.66e-15 73.2
Msa0861260 AT4G12850.2 38.739 111 64 3 50 160 4 110 1.11e-14 71.6
Msa0861260 AT1G10240.2 29.714 175 120 2 210 383 432 604 7.10e-14 75.1
Msa0861260 AT1G10240.2 31.507 146 91 5 51 189 42 185 3.59e-13 72.8
Msa0861260 AT1G10240.1 29.714 175 120 2 210 383 432 604 7.10e-14 75.1
Msa0861260 AT1G10240.1 31.507 146 91 5 51 189 42 185 3.59e-13 72.8
Msa0861260 AT3G07500.1 40.000 105 62 1 57 161 33 136 1.25e-13 70.9
Msa0861260 AT2G43280.1 38.318 107 58 3 57 159 22 124 7.95e-13 68.2
Msa0861260 AT1G52520.1 27.273 198 116 6 209 386 449 638 5.20e-12 69.3
Msa0861260 AT5G28530.4 28.249 177 115 4 210 381 384 553 1.74e-11 67.4
Msa0861260 AT5G28530.1 28.249 177 115 4 210 381 447 616 1.87e-11 67.4
Msa0861260 AT5G28530.5 30.719 153 94 4 240 381 398 549 2.67e-11 66.6
Msa0861260 AT5G28530.3 30.719 153 94 4 240 381 461 612 2.80e-11 66.6
Msa0861260 AT5G28530.2 30.719 153 94 4 240 381 461 612 2.80e-11 66.6

Find 101 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTCAGTATATAGGTTTC+AGG 0.197476 5_4:+60533555 None:intergenic
TTTGAAGTCTGATTTAAATT+TGG 0.211881 5_4:-60533955 Msa0861260:CDS
TTCAGATTGTGTCAATAAAT+TGG 0.215486 5_4:-60534886 Msa0861260:intron
TTCATCATTTGCAATTGATA+TGG 0.235636 5_4:+60533278 None:intergenic
CACTTTAAAAGAGCTGTTAA+TGG 0.282185 5_4:-60533918 Msa0861260:CDS
TTTGGTATTCGTCGAGAATA+TGG 0.287007 5_4:-60534729 Msa0861260:CDS
ATATATTCATGCAAATCTTT+TGG 0.296908 5_4:+60534402 None:intergenic
CTTGACTTGGTTATGACTCT+TGG 0.305137 5_4:+60532931 None:intergenic
TCTGCTCTAGGCTCCCCTTT+TGG 0.322934 5_4:+60534618 None:intergenic
AATGAGTATAGTAAAAGAAT+TGG 0.336174 5_4:-60534753 Msa0861260:CDS
TGGAACTCCTCAAATATTCT+AGG 0.341058 5_4:+60533789 None:intergenic
CATTGTACCGCTTCATGAAA+TGG 0.342244 5_4:+60533460 None:intergenic
ATGAGTTTCTGAAGCTTTGT+TGG 0.343120 5_4:+60533410 None:intergenic
ATTGTCTTGGGAGATGAATC+TGG 0.347852 5_4:-60534432 Msa0861260:CDS
TTCACCAGATCTCTTTCTCT+TGG 0.356018 5_4:+60533134 None:intergenic
CTGTATTCCACCAGCTTGCT+TGG 0.359827 5_4:+60534377 None:intergenic
GGAAATAAAAGCAGAGTTGA+TGG 0.361988 5_4:-60534708 Msa0861260:CDS
GATATTCTTCATATTCCTCT+TGG 0.370740 5_4:+60533769 None:intergenic
TGCCAACAATTTACCTCACT+TGG 0.380095 5_4:-60534817 Msa0861260:CDS
TATAGTAAAAGAATTGGATT+TGG 0.393932 5_4:-60534747 Msa0861260:CDS
TGCGTAAAAGATTTGAAAGA+AGG 0.394307 5_4:-60533738 Msa0861260:CDS
AGAAATTAGAACAGGATGTT+TGG 0.398022 5_4:-60534598 Msa0861260:CDS
TGTCCATCGCTTCAGTATAT+AGG 0.401552 5_4:+60533548 None:intergenic
TTACACTCCTAGAATATTTG+AGG 0.404149 5_4:-60533796 Msa0861260:CDS
GCTGGACTCTTCATAAACTT+TGG 0.408472 5_4:+60533368 None:intergenic
CCTGTTCTAATTTCTGCTCT+AGG 0.415588 5_4:+60534606 None:intergenic
AATTTAAATCAGACTTCAAA+TGG 0.428063 5_4:+60533958 None:intergenic
AACAGGATGTTTGGCGCGTA+TGG 0.429347 5_4:-60534589 Msa0861260:CDS
AAATTTAGTACAGTCGTTAC+CGG 0.437312 5_4:-60533112 Msa0861260:intron
ATTGTACCGCTTCATGAAAT+GGG 0.445421 5_4:+60533461 None:intergenic
AACAACAATGGCAGCGGCGC+AGG 0.452252 5_4:+60535362 None:intergenic
GGATGGTCGGAGACGGCGGC+GGG 0.454411 5_4:+60535384 None:intergenic
GACAAAAGACGTCAACCAAA+AGG 0.461065 5_4:-60534633 Msa0861260:CDS
GGGATGGTCGGAGACGGCGG+CGG 0.462743 5_4:+60535383 None:intergenic
TAGTGTTGGTAGCAGGATGA+TGG 0.465525 5_4:+60535412 None:intergenic
CCAACGGAGAGGATAGTTAT+GGG 0.468436 5_4:-60533687 Msa0861260:CDS
GGTTCGTTAGTGTTGGTAGC+AGG 0.468658 5_4:+60535405 None:intergenic
CCCATAACTATCCTCTCCGT+TGG 0.473881 5_4:+60533687 None:intergenic
TCCAACGGAGAGGATAGTTA+TGG 0.475548 5_4:-60533688 Msa0861260:CDS
GCTGCCATTGTTGTTGATCA+AGG 0.480338 5_4:-60535354 Msa0861260:intron
GGTTGGGAAGTGATCAAGAT+TGG 0.482271 5_4:-60533515 Msa0861260:CDS
CAAAATAATTGATGATATGT+TGG 0.486221 5_4:-60533346 Msa0861260:CDS
TTGGTTAACTTAACCATTCG+AGG 0.487402 5_4:+60533429 None:intergenic
AGTGTTGGTAGCAGGATGAT+GGG 0.490017 5_4:+60535413 None:intergenic
TTCCAAGTGAGGTAAATTGT+TGG 0.492887 5_4:+60534815 None:intergenic
ACAAAAGACGTCAACCAAAA+GGG 0.496414 5_4:-60534632 Msa0861260:CDS
GATAGTTATGGGAAATGCTA+TGG 0.498511 5_4:-60533676 Msa0861260:CDS
ACAAGCATCACAAATTGTCT+TGG 0.503759 5_4:-60534445 Msa0861260:CDS
GACACCGTTGGTATGGCAAA+AGG 0.506522 5_4:-60533243 Msa0861260:CDS
CGAATCATATGAACATGCTC+AGG 0.508001 5_4:+60534492 None:intergenic
TGCTCCAAGAGAAAGAGATC+TGG 0.508313 5_4:-60533138 Msa0861260:CDS
CCTAGAGCAGAAATTAGAAC+AGG 0.510209 5_4:-60534606 Msa0861260:CDS
GGCAGCGGCGCAGGGATGGT+CGG 0.512257 5_4:+60535371 None:intergenic
TGGACAAGAAATGCAAGGTT+GGG 0.513978 5_4:-60533531 Msa0861260:CDS
ATGGACAAGAAATGCAAGGT+TGG 0.517510 5_4:-60533532 Msa0861260:CDS
CAAGCATCACAAATTGTCTT+GGG 0.518135 5_4:-60534444 Msa0861260:CDS
GGAAATGAAGTTTGATTCTG+AGG 0.525153 5_4:-60534796 Msa0861260:CDS
CAATGGCAGCGGCGCAGGGA+TGG 0.526019 5_4:+60535367 None:intergenic
ATCGAGAAAGTTCACGCAAG+CGG 0.527366 5_4:-60533212 Msa0861260:CDS
GGCGCAGGGATGGTCGGAGA+CGG 0.533543 5_4:+60535377 None:intergenic
AATGGGCATGGACATATGTC+CGG 0.534093 5_4:-60534046 Msa0861260:CDS
GAAGTTTGATTCTGAGGCAG+CGG 0.545649 5_4:-60534790 Msa0861260:CDS
GCCTAGATTGAAAATGAAGA+AGG 0.546195 5_4:-60533847 Msa0861260:CDS
GCGACACAATCAAGTCAAGT+TGG 0.547894 5_4:-60532981 Msa0861260:CDS
CAATGTGGACACCGTTGGTA+TGG 0.550134 5_4:-60533250 Msa0861260:CDS
GAAGTGATCAAGATTGGAAA+GGG 0.552959 5_4:-60533509 Msa0861260:CDS
GTCCGGAAAACATTCACTGC+AGG 0.554763 5_4:-60534029 Msa0861260:CDS
GCAGGGATGGTCGGAGACGG+CGG 0.559162 5_4:+60535380 None:intergenic
ACAACAATGGCAGCGGCGCA+GGG 0.575609 5_4:+60535363 None:intergenic
TCAAACTTCATTTCCAAGTG+AGG 0.576931 5_4:+60534804 None:intergenic
GGCGGCGGGTTCGTTAGTGT+TGG 0.578521 5_4:+60535398 None:intergenic
CAAAATGTGCAACACGGACA+AGG 0.581561 5_4:-60534846 Msa0861260:CDS
GTGTTGGTAGCAGGATGATG+GGG 0.582430 5_4:+60535414 None:intergenic
GGAAGTGATCAAGATTGGAA+AGG 0.584639 5_4:-60533510 Msa0861260:CDS
GTATGGTTATTTCACTCGAT+CGG 0.586251 5_4:-60534572 Msa0861260:CDS
GGATGGAGAATCAGAGGGCA+TGG 0.588066 5_4:-60533310 Msa0861260:CDS
TATATATCCAAGCAAGCTGG+TGG 0.588298 5_4:-60534384 Msa0861260:CDS
CAATGAGTTATGTCCTCGAA+TGG 0.589590 5_4:-60533442 Msa0861260:CDS
GAATATATATCCAAGCAAGC+TGG 0.590655 5_4:-60534387 Msa0861260:CDS
AAACCTATATACTGAAGCGA+TGG 0.592444 5_4:-60533551 Msa0861260:CDS
CATGCTCAGGTTGAAGTTGG+TGG 0.600927 5_4:+60534505 None:intergenic
GTATGTGGATGGAGAATCAG+AGG 0.603406 5_4:-60533316 Msa0861260:CDS
CAAAAGACGTCAACCAAAAG+GGG 0.607006 5_4:-60534631 Msa0861260:CDS
GAACATGCTCAGGTTGAAGT+TGG 0.607804 5_4:+60534502 None:intergenic
TAAAAGCCCATTTCATGAAG+CGG 0.608976 5_4:-60533467 Msa0861260:CDS
TGTCGTAAATTTGAGACACA+TGG 0.610330 5_4:-60533633 Msa0861260:CDS
CAATCAAGTCAAGTTGGCAA+TGG 0.617634 5_4:-60532975 Msa0861260:CDS
AAGATTCACATGCTACAAAG+AGG 0.619398 5_4:-60534670 Msa0861260:CDS
TTGATCAACAACAATGGCAG+CGG 0.627743 5_4:+60535356 None:intergenic
GTGATCAAGATTGGAAAGGG+AGG 0.630069 5_4:-60533506 Msa0861260:CDS
TGCTACAAAGAGGGTGTACG+AGG 0.638548 5_4:-60534660 Msa0861260:CDS
AGCGATGGACAAGAAATGCA+AGG 0.651903 5_4:-60533536 Msa0861260:CDS
AGATTCACATGCTACAAAGA+GGG 0.654496 5_4:-60534669 Msa0861260:CDS
CACAAACTATGATAATCCAA+CGG 0.657053 5_4:-60533703 Msa0861260:CDS
CATGAACAAAATGTGCAACA+CGG 0.658217 5_4:-60534852 Msa0861260:CDS
ATTGACAATGTGGACACCGT+TGG 0.671830 5_4:-60533255 Msa0861260:CDS
ATTACCTTGATCAACAACAA+TGG 0.673823 5_4:+60535350 None:intergenic
AATATTTGAGGAGTTCCAAG+AGG 0.693235 5_4:-60533784 Msa0861260:CDS
AAATGATGAAATTGACAATG+TGG 0.705740 5_4:-60533265 Msa0861260:CDS
TATGTGGATGGAGAATCAGA+GGG 0.721169 5_4:-60533315 Msa0861260:CDS
ACTATGATAATCCAACGGAG+AGG 0.733483 5_4:-60533698 Msa0861260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACTATTAAAAAAACATT+GGG + chr5_4:60533222-60533241 None:intergenic 10.0%
!! TATTACTATTAAAAAAACAT+TGG + chr5_4:60533223-60533242 None:intergenic 10.0%
!! GAAAAGATAAATAAATAATA+TGG + chr5_4:60535332-60535351 None:intergenic 10.0%
!!! TACATATATATATAGCTTTA+TGG - chr5_4:60533157-60533176 Msa0861260:CDS 15.0%
!! TTACTATTAAAAAAACATTG+GGG + chr5_4:60533221-60533240 None:intergenic 15.0%
!! AAAACCAAAATTTACAATTA+AGG + chr5_4:60533458-60533477 None:intergenic 15.0%
!!! TTGATGAAAAATTAGTTTCT+AGG - chr5_4:60533100-60533119 Msa0861260:intron 20.0%
!! TACTATTAAAAAAACATTGG+GGG + chr5_4:60533220-60533239 None:intergenic 20.0%
!!! AATAGTAATAGATAGCTTTA+TGG - chr5_4:60533233-60533252 Msa0861260:CDS 20.0%
!! AATGAGTATAGTAAAAGAAT+TGG - chr5_4:60533611-60533630 Msa0861260:CDS 20.0%
!! TATAGTAAAAGAATTGGATT+TGG - chr5_4:60533617-60533636 Msa0861260:CDS 20.0%
!!! ATATATTCATGCAAATCTTT+TGG + chr5_4:60533965-60533984 None:intergenic 20.0%
!! TTTATCAATTGAAGGAAAAA+TGG - chr5_4:60534300-60534319 Msa0861260:intron 20.0%
!! TTATCAATTGAAGGAAAAAT+GGG - chr5_4:60534301-60534320 Msa0861260:intron 20.0%
!! AATTTAAATCAGACTTCAAA+TGG + chr5_4:60534409-60534428 None:intergenic 20.0%
!!! TTTGAAGTCTGATTTAAATT+TGG - chr5_4:60534409-60534428 Msa0861260:CDS 20.0%
!!! CAAAATAATTGATGATATGT+TGG - chr5_4:60535018-60535037 Msa0861260:intron 20.0%
!! TAAATAATATGGTTTCTTAC+CGG + chr5_4:60535321-60535340 None:intergenic 20.0%
! GGCAAATAATAAAATAGAGA+AGG + chr5_4:60533048-60533067 None:intergenic 25.0%
! AACAACATCATGTAATAAAG+AGG + chr5_4:60533072-60533091 None:intergenic 25.0%
! TTCAGATTGTGTCAATAAAT+TGG - chr5_4:60533478-60533497 Msa0861260:CDS 25.0%
! GTTCTATTATGACTTTCAAA+TGG - chr5_4:60534232-60534251 Msa0861260:intron 25.0%
! TGTTGAAGAGCAAATAATAT+TGG - chr5_4:60534256-60534275 Msa0861260:intron 25.0%
! AAGAGCAAATAATATTGGAA+GGG - chr5_4:60534261-60534280 Msa0861260:intron 25.0%
! GCATACAATTTATCAATTGA+AGG - chr5_4:60534292-60534311 Msa0861260:intron 25.0%
!!! CATATCATCAATTATTTTGC+TGG + chr5_4:60535017-60535036 None:intergenic 25.0%
! TTCATCATTTGCAATTGATA+TGG + chr5_4:60535089-60535108 None:intergenic 25.0%
! AAATGATGAAATTGACAATG+TGG - chr5_4:60535099-60535118 Msa0861260:intron 25.0%
! CATAGATAAAAACTACTTAC+CGG + chr5_4:60535274-60535293 None:intergenic 25.0%
ATTACCTTGATCAACAACAA+TGG + chr5_4:60533017-60533036 None:intergenic 30.0%
AACATCATGTAATAAAGAGG+CGG + chr5_4:60533069-60533088 None:intergenic 30.0%
TTAAAAAAACATTGGGGGAA+AGG + chr5_4:60533215-60533234 None:intergenic 30.0%
!! GTGGCCTTAATTGTAAATTT+TGG - chr5_4:60533451-60533470 Msa0861260:CDS 30.0%
AGAAATTAGAACAGGATGTT+TGG - chr5_4:60533766-60533785 Msa0861260:CDS 30.0%
TGAAAGTCATAATAGAACGA+GGG + chr5_4:60534230-60534249 None:intergenic 30.0%
TTGAAAGTCATAATAGAACG+AGG + chr5_4:60534231-60534250 None:intergenic 30.0%
GAAGAGCAAATAATATTGGA+AGG - chr5_4:60534260-60534279 Msa0861260:intron 30.0%
! CACTTTAAAAGAGCTGTTAA+TGG - chr5_4:60534446-60534465 Msa0861260:CDS 30.0%
TTACACTCCTAGAATATTTG+AGG - chr5_4:60534568-60534587 Msa0861260:CDS 30.0%
GATATTCTTCATATTCCTCT+TGG + chr5_4:60534598-60534617 None:intergenic 30.0%
TGCGTAAAAGATTTGAAAGA+AGG - chr5_4:60534626-60534645 Msa0861260:CDS 30.0%
CACAAACTATGATAATCCAA+CGG - chr5_4:60534661-60534680 Msa0861260:CDS 30.0%
GGAAAAAGCGATATAAATCT+TGG - chr5_4:60535173-60535192 Msa0861260:intron 30.0%
AAATTTAGTACAGTCGTTAC+CGG - chr5_4:60535252-60535271 Msa0861260:intron 30.0%
!! AATTTTAGTACAGTCGTTAC+CGG - chr5_4:60535299-60535318 Msa0861260:intron 30.0%
! ATGCAGTCTAAGTTTTCTAG+TGG - chr5_4:60533269-60533288 Msa0861260:CDS 35.0%
!! AAAACAACATTCTGAAGTCG+CGG + chr5_4:60533377-60533396 None:intergenic 35.0%
! ATGTCTGAAATCGTCACTTA+TGG - chr5_4:60533429-60533448 Msa0861260:CDS 35.0%
CATGAACAAAATGTGCAACA+CGG - chr5_4:60533512-60533531 Msa0861260:CDS 35.0%
TTCCAAGTGAGGTAAATTGT+TGG + chr5_4:60533552-60533571 None:intergenic 35.0%
TCAAACTTCATTTCCAAGTG+AGG + chr5_4:60533563-60533582 None:intergenic 35.0%
GGAAATGAAGTTTGATTCTG+AGG - chr5_4:60533568-60533587 Msa0861260:CDS 35.0%
TTTGGTATTCGTCGAGAATA+TGG - chr5_4:60533635-60533654 Msa0861260:CDS 35.0%
GGAAATAAAAGCAGAGTTGA+TGG - chr5_4:60533656-60533675 Msa0861260:CDS 35.0%
AAGATTCACATGCTACAAAG+AGG - chr5_4:60533694-60533713 Msa0861260:CDS 35.0%
AGATTCACATGCTACAAAGA+GGG - chr5_4:60533695-60533714 Msa0861260:CDS 35.0%
ACAAAAGACGTCAACCAAAA+GGG - chr5_4:60533732-60533751 Msa0861260:CDS 35.0%
! GTATGGTTATTTCACTCGAT+CGG - chr5_4:60533792-60533811 Msa0861260:CDS 35.0%
ACAAGCATCACAAATTGTCT+TGG - chr5_4:60533919-60533938 Msa0861260:CDS 35.0%
CAAGCATCACAAATTGTCTT+GGG - chr5_4:60533920-60533939 Msa0861260:CDS 35.0%
GAATATATATCCAAGCAAGC+TGG - chr5_4:60533977-60533996 Msa0861260:CDS 35.0%
AGGAAACAGTCACTAAAGTA+TGG - chr5_4:60534158-60534177 Msa0861260:intron 35.0%
AAATAATATTGGAAGGGTCG+TGG - chr5_4:60534267-60534286 Msa0861260:intron 35.0%
AATTGAAGGAAAAATGGGCA+TGG - chr5_4:60534306-60534325 Msa0861260:intron 35.0%
GCCTAGATTGAAAATGAAGA+AGG - chr5_4:60534517-60534536 Msa0861260:CDS 35.0%
! GCCTTCTTCATTTTCAATCT+AGG + chr5_4:60534521-60534540 None:intergenic 35.0%
TGGAACTCCTCAAATATTCT+AGG + chr5_4:60534578-60534597 None:intergenic 35.0%
AATATTTGAGGAGTTCCAAG+AGG - chr5_4:60534580-60534599 Msa0861260:CDS 35.0%
GATAGTTATGGGAAATGCTA+TGG - chr5_4:60534688-60534707 Msa0861260:CDS 35.0%
! TGTCGTAAATTTGAGACACA+TGG - chr5_4:60534731-60534750 Msa0861260:CDS 35.0%
AAACCTATATACTGAAGCGA+TGG - chr5_4:60534813-60534832 Msa0861260:CDS 35.0%
GAAGTGATCAAGATTGGAAA+GGG - chr5_4:60534855-60534874 Msa0861260:CDS 35.0%
TAAAAGCCCATTTCATGAAG+CGG - chr5_4:60534897-60534916 Msa0861260:intron 35.0%
ATTGTACCGCTTCATGAAAT+GGG + chr5_4:60534906-60534925 None:intergenic 35.0%
TTGGTTAACTTAACCATTCG+AGG + chr5_4:60534938-60534957 None:intergenic 35.0%
!! ATGAGTTTCTGAAGCTTTGT+TGG + chr5_4:60534957-60534976 None:intergenic 35.0%
! TATGTTGGCCAAAAAGTATG+TGG - chr5_4:60535033-60535052 Msa0861260:intron 35.0%
! TTCTCCATCCACATACTTTT+TGG + chr5_4:60535044-60535063 None:intergenic 35.0%
TTGATCAACAACAATGGCAG+CGG + chr5_4:60533011-60533030 None:intergenic 40.0%
AAAAACATTGGGGGAAAGGA+TGG + chr5_4:60533211-60533230 None:intergenic 40.0%
! TCTGAAATCGTCACTTATGG+TGG - chr5_4:60533432-60533451 Msa0861260:CDS 40.0%
TGCCAACAATTTACCTCACT+TGG - chr5_4:60533547-60533566 Msa0861260:CDS 40.0%
GACAAAAGACGTCAACCAAA+AGG - chr5_4:60533731-60533750 Msa0861260:CDS 40.0%
CAAAAGACGTCAACCAAAAG+GGG - chr5_4:60533733-60533752 Msa0861260:CDS 40.0%
CCTGTTCTAATTTCTGCTCT+AGG + chr5_4:60533761-60533780 None:intergenic 40.0%
CCTAGAGCAGAAATTAGAAC+AGG - chr5_4:60533758-60533777 Msa0861260:CDS 40.0%
CGAATCATATGAACATGCTC+AGG + chr5_4:60533875-60533894 None:intergenic 40.0%
ATTGTCTTGGGAGATGAATC+TGG - chr5_4:60533932-60533951 Msa0861260:CDS 40.0%
TATATATCCAAGCAAGCTGG+TGG - chr5_4:60533980-60533999 Msa0861260:CDS 40.0%
ACAGTCACTAAAGTATGGTG+AGG - chr5_4:60534163-60534182 Msa0861260:intron 40.0%
TCACTAAAGTATGGTGAGGT+GGG - chr5_4:60534167-60534186 Msa0861260:intron 40.0%
! TACCTGCAGTGAATGTTTTC+CGG + chr5_4:60534340-60534359 None:intergenic 40.0%
ACTATGATAATCCAACGGAG+AGG - chr5_4:60534666-60534685 Msa0861260:CDS 40.0%
CGCTTCAGTATATAGGTTTC+AGG + chr5_4:60534812-60534831 None:intergenic 40.0%
TGTCCATCGCTTCAGTATAT+AGG + chr5_4:60534819-60534838 None:intergenic 40.0%
ATGGACAAGAAATGCAAGGT+TGG - chr5_4:60534832-60534851 Msa0861260:CDS 40.0%
TGGACAAGAAATGCAAGGTT+GGG - chr5_4:60534833-60534852 Msa0861260:CDS 40.0%
GGAAGTGATCAAGATTGGAA+AGG - chr5_4:60534854-60534873 Msa0861260:CDS 40.0%
CATTGTACCGCTTCATGAAA+TGG + chr5_4:60534907-60534926 None:intergenic 40.0%
CAATGAGTTATGTCCTCGAA+TGG - chr5_4:60534922-60534941 Msa0861260:intron 40.0%
GCTGGACTCTTCATAAACTT+TGG + chr5_4:60534999-60535018 None:intergenic 40.0%
! TTGGCCAAAAAGTATGTGGA+TGG - chr5_4:60535037-60535056 Msa0861260:intron 40.0%
TATGTGGATGGAGAATCAGA+GGG - chr5_4:60535049-60535068 Msa0861260:intron 40.0%
! TATGCCTTTTGCCATACCAA+CGG + chr5_4:60535128-60535147 None:intergenic 40.0%
TTCACCAGATCTCTTTCTCT+TGG + chr5_4:60535233-60535252 None:intergenic 40.0%
!! CAATCAAGTCAAGTTGGCAA+TGG - chr5_4:60535389-60535408 Msa0861260:CDS 40.0%
!! AGTGTTGGTAGCAGGATGAT+GGG + chr5_4:60532954-60532973 None:intergenic 45.0%
!! TAGTGTTGGTAGCAGGATGA+TGG + chr5_4:60532955-60532974 None:intergenic 45.0%
! GCTGCCATTGTTGTTGATCA+AGG - chr5_4:60533010-60533029 Msa0861260:intron 45.0%
CAAAATGTGCAACACGGACA+AGG - chr5_4:60533518-60533537 Msa0861260:CDS 45.0%
!! GAAGTTTGATTCTGAGGCAG+CGG - chr5_4:60533574-60533593 Msa0861260:CDS 45.0%
!! GAACATGCTCAGGTTGAAGT+TGG + chr5_4:60533865-60533884 None:intergenic 45.0%
GTCACTAAAGTATGGTGAGG+TGG - chr5_4:60534166-60534185 Msa0861260:intron 45.0%
AATGGGCATGGACATATGTC+CGG - chr5_4:60534318-60534337 Msa0861260:intron 45.0%
TCCAACGGAGAGGATAGTTA+TGG - chr5_4:60534676-60534695 Msa0861260:CDS 45.0%
CCAACGGAGAGGATAGTTAT+GGG - chr5_4:60534677-60534696 Msa0861260:CDS 45.0%
AGCGATGGACAAGAAATGCA+AGG - chr5_4:60534828-60534847 Msa0861260:CDS 45.0%
GGTTGGGAAGTGATCAAGAT+TGG - chr5_4:60534849-60534868 Msa0861260:CDS 45.0%
GTGATCAAGATTGGAAAGGG+AGG - chr5_4:60534858-60534877 Msa0861260:CDS 45.0%
GTATGTGGATGGAGAATCAG+AGG - chr5_4:60535048-60535067 Msa0861260:intron 45.0%
ATTGACAATGTGGACACCGT+TGG - chr5_4:60535109-60535128 Msa0861260:intron 45.0%
ATCGAGAAAGTTCACGCAAG+CGG - chr5_4:60535152-60535171 Msa0861260:intron 45.0%
TGCTCCAAGAGAAAGAGATC+TGG - chr5_4:60535226-60535245 Msa0861260:intron 45.0%
GCGACACAATCAAGTCAAGT+TGG - chr5_4:60535383-60535402 Msa0861260:CDS 45.0%
!! GTGTTGGTAGCAGGATGATG+GGG + chr5_4:60532953-60532972 None:intergenic 50.0%
!! GGTTCGTTAGTGTTGGTAGC+AGG + chr5_4:60532962-60532981 None:intergenic 50.0%
! TGCTACAAAGAGGGTGTACG+AGG - chr5_4:60533704-60533723 Msa0861260:CDS 50.0%
AACAGGATGTTTGGCGCGTA+TGG - chr5_4:60533775-60533794 Msa0861260:CDS 50.0%
!! CATGCTCAGGTTGAAGTTGG+TGG + chr5_4:60533862-60533881 None:intergenic 50.0%
! CTGTATTCCACCAGCTTGCT+TGG + chr5_4:60533990-60534009 None:intergenic 50.0%
GCTGGTGGAATACAGACAGT+TGG - chr5_4:60533995-60534014 Msa0861260:CDS 50.0%
GTCCGGAAAACATTCACTGC+AGG - chr5_4:60534335-60534354 Msa0861260:intron 50.0%
CCCATAACTATCCTCTCCGT+TGG + chr5_4:60534680-60534699 None:intergenic 50.0%
CAATGTGGACACCGTTGGTA+TGG - chr5_4:60535114-60535133 Msa0861260:intron 50.0%
GACACCGTTGGTATGGCAAA+AGG - chr5_4:60535121-60535140 Msa0861260:intron 50.0%
!! TCTGCTCTAGGCTCCCCTTT+TGG + chr5_4:60533749-60533768 None:intergenic 55.0%
GGATGGAGAATCAGAGGGCA+TGG - chr5_4:60535054-60535073 Msa0861260:intron 55.0%
ACAACAATGGCAGCGGCGCA+GGG + chr5_4:60533004-60533023 None:intergenic 60.0%
AACAACAATGGCAGCGGCGC+AGG + chr5_4:60533005-60533024 None:intergenic 60.0%
! GGCGGCGGGTTCGTTAGTGT+TGG + chr5_4:60532969-60532988 None:intergenic 65.0%
GCAGGGATGGTCGGAGACGG+CGG + chr5_4:60532987-60533006 None:intergenic 70.0%
GGCGCAGGGATGGTCGGAGA+CGG + chr5_4:60532990-60533009 None:intergenic 70.0%
CAATGGCAGCGGCGCAGGGA+TGG + chr5_4:60533000-60533019 None:intergenic 70.0%
GGATGGTCGGAGACGGCGGC+GGG + chr5_4:60532983-60533002 None:intergenic 75.0%
GGGATGGTCGGAGACGGCGG+CGG + chr5_4:60532984-60533003 None:intergenic 75.0%
GGCAGCGGCGCAGGGATGGT+CGG + chr5_4:60532996-60533015 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 60532941 60535445 60532941 ID=Msa0861260;Name=Msa0861260
chr5_4 mRNA 60532941 60535445 60532941 ID=Msa0861260-mRNA-1;Parent=Msa0861260;Name=Msa0861260-mRNA-1;_AED=0.12;_eAED=0.15;_QI=0|0|0|1|1|1|4|0|553
chr5_4 exon 60535355 60535445 60535355 ID=Msa0861260-mRNA-1:exon:18703;Parent=Msa0861260-mRNA-1
chr5_4 exon 60534374 60534903 60534374 ID=Msa0861260-mRNA-1:exon:18702;Parent=Msa0861260-mRNA-1
chr5_4 exon 60533113 60534072 60533113 ID=Msa0861260-mRNA-1:exon:18701;Parent=Msa0861260-mRNA-1
chr5_4 exon 60532941 60533021 60532941 ID=Msa0861260-mRNA-1:exon:18700;Parent=Msa0861260-mRNA-1
chr5_4 CDS 60535355 60535445 60535355 ID=Msa0861260-mRNA-1:cds;Parent=Msa0861260-mRNA-1
chr5_4 CDS 60534374 60534903 60534374 ID=Msa0861260-mRNA-1:cds;Parent=Msa0861260-mRNA-1
chr5_4 CDS 60533113 60534072 60533113 ID=Msa0861260-mRNA-1:cds;Parent=Msa0861260-mRNA-1
chr5_4 CDS 60532941 60533021 60532941 ID=Msa0861260-mRNA-1:cds;Parent=Msa0861260-mRNA-1
Gene Sequence

>Msa0861260

ATGAGCGAACCCCATCATCCTGCTACCAACACTAACGAACCCGCCGCCGTCTCCGACCATCCCTGCGCCGCTGCCATTGTTGTTGATCAAGATTGTGTCAATAAATTGGTTAACAACATTCATGAACAAAATGTGCAACACGGACAAGGAAGTATTGCCAACAATTTACCTCACTTGGAAATGAAGTTTGATTCTGAGGCAGCGGCTTATGACTTTTATAATGAGTATAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAGTTGATGGAGTTTTGACTTCAAGAAGATTCACATGCTACAAAGAGGGTGTACGAGGTGTTGACAAAAGACGTCAACCAAAAGGGGAGCCTAGAGCAGAAATTAGAACAGGATGTTTGGCGCGTATGGTTATTTCACTCGATCGGAAGATAGAGAAATATAAAGTTGTTGACTTTATAGCTGAACATAACCACCAACTTCAACCTGAGCATGTTCATATGATTCGCTCTCATCGACGCATATCTGAGACACAAGCATCACAAATTGTCTTGGGAGATGAATCTGGATTAAGACCAAAAGATTTGCATGAATATATATCCAAGCAAGCTGGTGGAATACAGACAGAAAAATGGGCATGGACATATGTCCGGAAAACATTCACTGCAGGTATGAGATCTACTCAATTAAGTGAGAGCTTTAATGCTGATTTGAAGAACCATTTGAAGTCTGATTTAAATTTGGTACAATTTTTTACACACTTTAAAAGAGCTGTTAATGGTAAACGAAACAAAGAGTCAGAAGCTGAATATGAGTCAAAACATAAGCTGCCTAGATTGAAAATGAAGAAGGCTCGTATGCTCATACAAGCTGCAAATGTTTACACTCCTAGAATATTTGAGGAGTTCCAAGAGGAATATGAAGAATATCAAGACACTTGCGTAAAAGATTTGAAAGAAGGTTTGTATGCTGTCACAAACTATGATAATCCAACGGAGAGGATAGTTATGGGAAATGCTATGGAACAAAAAGTTTCATGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGTAGTCATGCTTTGAAAGTTTTAGATGTCATGAACATTAAGTTAATTCCTGAAACCTATATACTGAAGCGATGGACAAGAAATGCAAGGTTGGGAAGTGATCAAGATTGGAAAGGGAGGCATGTTGAATTAGACATAAAAGCCCATTTCATGAAGCGGTACAATGAGTTATGTCCTCGAATGGTTAAGTTAACCAACAAAGCTTCAGAAACTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAATTGATGATATGTTGGCCAAAAAGTATGTGGATGGAGAATCAGAGGGCATGGTTCATGTATCCATATCAATTGCAAATGATGAAATTGACAATGTGGACACCGTTGGTATGGCAAAAGGCATAGCTAATCGAGAAAGTTCACGCAAGCGGAAAAAGCGATATAAATCTTGGATTGAGAAAAAAGATAAAAAGACAAAGATGTGCTCCAAGAGAAAGAGATCTGGTGAAAATTTAGTACAGTCGTTACCGCAAGAAACTACAGTTCAAGCGACACAATCAAGTCAAGTTGGCAATGGAACTCAACATCAAGCTACAATCCAAGAGTCATAA
Protein sequence

>Msa0861260

MSEPHHPATNTNEPAAVSDHPCAAAIVVDQDCVNKLVNNIHEQNVQHGQGSIANNLPHLEMKFDSEAAAYDFYNEYSKRIGFGIRREYGNKSRVDGVLTSRRFTCYKEGVRGVDKRRQPKGEPRAEIRTGCLARMVISLDRKIEKYKVVDFIAEHNHQLQPEHVHMIRSHRRISETQASQIVLGDESGLRPKDLHEYISKQAGGIQTEKWAWTYVRKTFTAGMRSTQLSESFNADLKNHLKSDLNLVQFFTHFKRAVNGKRNKESEAEYESKHKLPRLKMKKARMLIQAANVYTPRIFEEFQEEYEEYQDTCVKDLKEGLYAVTNYDNPTERIVMGNAMEQKVSCDCRKFETHGILCSHALKVLDVMNIKLIPETYILKRWTRNARLGSDQDWKGRHVELDIKAHFMKRYNELCPRMVKLTNKASETHETYTFLSKVYEESSKIIDDMLAKKYVDGESEGMVHVSISIANDEIDNVDTVGMAKGIANRESSRKRKKRYKSWIEKKDKKTKMCSKRKRSGENLVQSLPQETTVQATQSSQVGNGTQHQATIQES*