AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0864560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0864560 MtrunA17_Chr5g0447111 93.636 330 19 1 1 328 1 330 0.0 638
Msa0864560 MtrunA17_Chr3g0103031 63.526 329 117 3 1 327 1 328 2.21e-149 423
Msa0864560 MtrunA17_Chr1g0159831 44.954 327 173 4 7 327 5 330 5.17e-99 295
Msa0864560 MtrunA17_Chr3g0124921 46.894 322 166 5 5 323 2 321 1.41e-98 294
Msa0864560 MtrunA17_Chr5g0447191 54.335 173 78 1 152 323 15 187 6.10e-59 187
Msa0864560 MtrunA17_Chr4g0029841 32.589 224 127 5 98 321 77 276 1.47e-26 106
Msa0864560 MtrunA17_Chr3g0114411 33.735 166 102 4 133 292 70 233 1.58e-12 67.0
Msa0864560 MtrunA17_Chr3g0125321 28.289 152 103 3 174 323 94 241 5.11e-12 65.1
Msa0864560 MtrunA17_Chr1g0185861 26.347 167 111 3 137 292 185 350 1.04e-11 65.5
Msa0864560 MtrunA17_Chr7g0259501 27.333 150 97 3 153 291 197 345 1.36e-11 65.1
Msa0864560 MtrunA17_Chr7g0259851 28.462 130 91 2 163 292 526 653 1.36e-11 65.5
Msa0864560 MtrunA17_Chr5g0394261 27.660 141 99 3 163 302 536 674 2.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0864560 AT1G55760.1 56.402 328 141 2 1 327 1 327 1.16e-134 386
Msa0864560 AT1G21780.2 48.447 322 163 3 7 327 5 324 1.80e-109 322
Msa0864560 AT1G21780.1 48.447 322 163 3 7 327 5 324 1.80e-109 322
Msa0864560 AT1G01640.1 33.333 150 97 3 145 292 6 154 5.71e-15 73.2
Msa0864560 AT3G56230.1 32.117 137 90 3 159 292 107 243 1.42e-13 70.5
Msa0864560 AT1G01640.2 32.836 134 87 3 161 292 1 133 2.57e-13 67.8
Msa0864560 AT2G30600.4 27.805 205 127 7 138 325 326 526 6.14e-13 70.1
Msa0864560 AT2G30600.1 27.805 205 127 7 138 325 326 526 6.14e-13 70.1
Msa0864560 AT2G30600.3 27.805 205 127 7 138 325 326 526 6.14e-13 70.1
Msa0864560 AT2G30600.2 27.805 205 127 7 138 325 326 526 6.14e-13 70.1
Msa0864560 AT2G30600.6 27.805 205 127 7 138 325 326 526 6.18e-13 70.1
Msa0864560 AT2G39760.2 28.049 164 104 3 139 291 172 332 1.03e-12 68.6
Msa0864560 AT2G39760.4 28.049 164 104 3 139 291 172 332 1.31e-12 68.2
Msa0864560 AT2G39760.3 28.049 164 104 3 139 291 172 332 1.31e-12 68.2
Msa0864560 AT2G39760.1 27.119 177 112 4 139 301 172 345 1.62e-12 68.2
Msa0864560 AT1G05690.1 26.012 173 114 6 137 301 29 195 3.71e-12 67.0
Msa0864560 AT4G08455.1 37.500 96 55 2 175 268 107 199 2.95e-11 63.5
Msa0864560 AT2G30600.5 29.252 147 94 4 138 279 326 467 4.02e-11 64.7

Find 66 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGGTAGTGCTCTTTATT+TGG 0.185967 5_4:+64961907 Msa0864560:three_prime_UTR
ATGCAATATCTTGTGAAATT+TGG 0.204904 5_4:+64961661 Msa0864560:CDS
TAGATAGGTTGTTTGGTTAT+TGG 0.217086 5_4:+64961840 Msa0864560:three_prime_UTR
ATTGATACAAAGATGCAATT+TGG 0.251499 5_4:-64961615 None:intergenic
TGACATGTCGGTGATGTTTA+TGG 0.257723 5_4:-64961413 None:intergenic
GTGGGGAAGCTTGCTCTATT+TGG 0.265550 5_4:+64961188 Msa0864560:CDS
GAAATACAAGAATTCTTCTA+AGG 0.281648 5_4:-64960363 None:intergenic
CCATTGAACTAGACGATTCA+TGG 0.288711 5_4:-64960417 None:intergenic
CAATTGCATCTTTCATAGTT+AGG 0.300214 5_4:+64960670 Msa0864560:CDS
GCTTGCTCTATTTGGCCTAA+TGG 0.324580 5_4:+64961196 Msa0864560:CDS
AGAATTTCTTATACATAGAT+TGG 0.343658 5_4:+64961489 Msa0864560:CDS
CTAAACATAGATAGGTTGTT+TGG 0.351077 5_4:+64961833 Msa0864560:three_prime_UTR
AGCAAAATCTTGACCCAATC+AGG 0.352906 5_4:+64960714 Msa0864560:CDS
ATAGCTTTCTGTGGGAGATT+GGG 0.358877 5_4:+64960894 Msa0864560:CDS
GATAGCTTTCTGTGGGAGAT+TGG 0.359309 5_4:+64960893 Msa0864560:CDS
ACTTGTCAGCCGCATGTAAT+AGG 0.362904 5_4:-64961513 None:intergenic
TCAAAGAAATTCTTGATGCT+TGG 0.368629 5_4:+64961758 Msa0864560:CDS
AGATAAACTTCTTAGCAATA+AGG 0.369571 5_4:+64960871 Msa0864560:CDS
TGATTTCAATGTATATATAT+AGG 0.375951 5_4:+64961890 Msa0864560:three_prime_UTR
GTTAGGATTCTTAGTTATGC+TGG 0.386999 5_4:+64960687 Msa0864560:CDS
AGGGTTGTGTGTGATTGTTT+TGG 0.394454 5_4:-64960646 None:intergenic
TCAAAATAGCCATGTGGAAC+TGG 0.403978 5_4:+64960486 Msa0864560:CDS
CGTTGATTGTTATGTCTGTA+TGG 0.409784 5_4:-64961285 None:intergenic
CACAATCCAAGTTGTCAACT+TGG 0.433648 5_4:-64960286 None:intergenic
AAAGAAAATTTATAAACGTC+TGG 0.434882 5_4:-64961447 None:intergenic
TTCTCCTATCATTCGACCTA+GGG 0.439636 5_4:-64961257 None:intergenic
CTAACTATGAAAGATGCAAT+TGG 0.440177 5_4:-64960669 None:intergenic
AGATTCTACTAAACATAGAT+AGG 0.442473 5_4:+64961825 Msa0864560:three_prime_UTR
CCATGAATCGTCTAGTTCAA+TGG 0.453483 5_4:+64960417 Msa0864560:CDS
TGTCAACTTGGAAACATGAT+AGG 0.466255 5_4:-64960274 None:intergenic
TAGAACTTGAATCACCTGAT+TGG 0.468839 5_4:-64960728 None:intergenic
TCATTCGACCTAGGGATTCA+AGG 0.469041 5_4:-64961249 None:intergenic
AGAACTTGAATCACCTGATT+GGG 0.473517 5_4:-64960727 None:intergenic
GAATCCCTAGGTCGAATGAT+AGG 0.475257 5_4:+64961253 Msa0864560:CDS
TAGCTTTCTGTGGGAGATTG+GGG 0.476737 5_4:+64960895 Msa0864560:CDS
GAGATTGGGGTTCCACTACC+TGG 0.484202 5_4:+64960908 Msa0864560:CDS
AGAAAGAAAGGAACAAAAGA+AGG 0.491789 5_4:-64960225 None:intergenic
ATGTTTCCAAGTTGACAACT+TGG 0.509102 5_4:+64960280 Msa0864560:five_prime_UTR
CTCGGCTGCATAAATCCATT+AGG 0.509151 5_4:-64961211 None:intergenic
GAAATAACATTGTCTAGGAC+AGG 0.509771 5_4:-64960203 None:intergenic
TTTCTCCTATCATTCGACCT+AGG 0.524682 5_4:-64961258 None:intergenic
GGCAAAATATATGATAATCA+AGG 0.534940 5_4:+64961682 Msa0864560:CDS
AGAAGGAAATAACATTGTCT+AGG 0.540582 5_4:-64960208 None:intergenic
CATTCGACCTAGGGATTCAA+GGG 0.553806 5_4:-64961248 None:intergenic
CAATAAGGATAGCTTTCTGT+GGG 0.562302 5_4:+64960886 Msa0864560:CDS
ATGAGCTCTTATGCTTCCCT+CGG 0.565616 5_4:-64961314 None:intergenic
ACTATGAAAGATGCAATTGG+AGG 0.571379 5_4:-64960666 None:intergenic
GCAGCGGCCCTTGAATCCCT+AGG 0.588324 5_4:+64961241 Msa0864560:CDS
AACAATCAACGTCAATTCCG+AGG 0.588705 5_4:+64961297 Msa0864560:CDS
AGTTGACAACTTGGATTGTG+AGG 0.593060 5_4:+64960289 Msa0864560:five_prime_UTR
GCAATAAGGATAGCTTTCTG+TGG 0.597072 5_4:+64960885 Msa0864560:CDS
TTTGAAAACTACATGTCCAA+AGG 0.597428 5_4:+64960961 Msa0864560:CDS
CTATGAAAGATGCAATTGGA+GGG 0.614089 5_4:-64960665 None:intergenic
GATATGAAGGCATTTATCAG+TGG 0.615660 5_4:+64960581 Msa0864560:intron
ACAATCAACGTCAATTCCGA+GGG 0.626764 5_4:+64961298 Msa0864560:CDS
TTGACTTTACCAGTTCCACA+TGG 0.646739 5_4:-64960495 None:intergenic
ATAACATTGTCTAGGACAGG+AGG 0.649366 5_4:-64960200 None:intergenic
TTATCAGTGGAAAAGAACTG+TGG 0.666641 5_4:+64960594 Msa0864560:CDS
CATTAGAGTGCTAAACACAA+AGG 0.666694 5_4:-64961937 None:intergenic
GAAAACATGCTGTGAAACAC+AGG 0.667859 5_4:-64961361 None:intergenic
GAAAGATTGAATTGACATGT+CGG 0.685189 5_4:-64961425 None:intergenic
TAGATTGGACCTATTACATG+CGG 0.685610 5_4:+64961504 Msa0864560:CDS
TGATATCTATGAGTTAAGAG+AGG 0.687061 5_4:+64961537 Msa0864560:CDS
ATCCATTCAAAATAGCCATG+TGG 0.702367 5_4:+64960480 Msa0864560:CDS
GTGAAACACAGGTGATTGTG+AGG 0.704876 5_4:-64961350 None:intergenic
GCAGCCGAGACAAAATGCAG+CGG 0.710020 5_4:+64961225 Msa0864560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAATATATCCAAATTA+AGG + chr5_4:64960992-64961011 Msa0864560:intron 10.0%
!! TATATAATATATCCAAATTA+AGG + chr5_4:64960992-64961011 Msa0864560:intron 10.0%
!! TATTATTCAATACCTTAATT+TGG - chr5_4:64961007-64961026 None:intergenic 15.0%
!! CAAACAAAATAAAAAGTAAA+TGG - chr5_4:64961057-64961076 None:intergenic 15.0%
!!! CATTTACTTTTTATTTTGTT+TGG + chr5_4:64961055-64961074 Msa0864560:intron 15.0%
!!! ATCAAGGAAAATTTTAAATT+AGG - chr5_4:64961157-64961176 None:intergenic 15.0%
!!! ACGTTTATAAATTTTCTTTA+TGG + chr5_4:64961451-64961470 Msa0864560:CDS 15.0%
!! AGAAAAAAATAAAACAATCA+TGG - chr5_4:64961870-64961889 None:intergenic 15.0%
!! TGATTTCAATGTATATATAT+AGG + chr5_4:64961890-64961909 Msa0864560:three_prime_UTR 15.0%
!! TATTATTCAATACCTTAATT+TGG - chr5_4:64961007-64961026 None:intergenic 15.0%
!! CAAACAAAATAAAAAGTAAA+TGG - chr5_4:64961057-64961076 None:intergenic 15.0%
!!! CATTTACTTTTTATTTTGTT+TGG + chr5_4:64961055-64961074 Msa0864560:intron 15.0%
!!! ATCAAGGAAAATTTTAAATT+AGG - chr5_4:64961157-64961176 None:intergenic 15.0%
!!! ACGTTTATAAATTTTCTTTA+TGG + chr5_4:64961451-64961470 Msa0864560:CDS 15.0%
!! AGAAAAAAATAAAACAATCA+TGG - chr5_4:64961870-64961889 None:intergenic 15.0%
!! TGATTTCAATGTATATATAT+AGG + chr5_4:64961890-64961909 Msa0864560:three_prime_UTR 15.0%
!!! TTTTTTCTTTCAAGAAAGAA+AGG - chr5_4:64960240-64960259 None:intergenic 20.0%
!!! ATGTCAATTATAAATTTTCC+AGG - chr5_4:64960929-64960948 None:intergenic 20.0%
!! ATTATATACTTACATACCTT+TGG - chr5_4:64960980-64960999 None:intergenic 20.0%
!!! ATAATCATAGTATTGATATG+AGG + chr5_4:64961108-64961127 Msa0864560:intron 20.0%
!!! TAATCATAGTATTGATATGA+GGG + chr5_4:64961109-64961128 Msa0864560:intron 20.0%
!! AAAGAAAATTTATAAACGTC+TGG - chr5_4:64961450-64961469 None:intergenic 20.0%
!! AGAATTTCTTATACATAGAT+TGG + chr5_4:64961489-64961508 Msa0864560:CDS 20.0%
!!! TCTTCCAAATTTTTAAAGAT+AGG + chr5_4:64961796-64961815 Msa0864560:three_prime_UTR 20.0%
!!! TCTTCCTATCTTTAAAAATT+TGG - chr5_4:64961803-64961822 None:intergenic 20.0%
!!! TTTTTTCTTTCAAGAAAGAA+AGG - chr5_4:64960240-64960259 None:intergenic 20.0%
!!! ATGTCAATTATAAATTTTCC+AGG - chr5_4:64960929-64960948 None:intergenic 20.0%
!! ATTATATACTTACATACCTT+TGG - chr5_4:64960980-64960999 None:intergenic 20.0%
!!! ATAATCATAGTATTGATATG+AGG + chr5_4:64961108-64961127 Msa0864560:intron 20.0%
!!! TAATCATAGTATTGATATGA+GGG + chr5_4:64961109-64961128 Msa0864560:intron 20.0%
!! AAAGAAAATTTATAAACGTC+TGG - chr5_4:64961450-64961469 None:intergenic 20.0%
!! AGAATTTCTTATACATAGAT+TGG + chr5_4:64961489-64961508 Msa0864560:CDS 20.0%
!!! TCTTCCAAATTTTTAAAGAT+AGG + chr5_4:64961796-64961815 Msa0864560:three_prime_UTR 20.0%
!!! TCTTCCTATCTTTAAAAATT+TGG - chr5_4:64961803-64961822 None:intergenic 20.0%
! GAAATACAAGAATTCTTCTA+AGG - chr5_4:64960366-64960385 None:intergenic 25.0%
!!! GTTTTGTTCTTATGATATGA+AGG + chr5_4:64960568-64960587 Msa0864560:intron 25.0%
! AGATAAACTTCTTAGCAATA+AGG + chr5_4:64960871-64960890 Msa0864560:CDS 25.0%
!! ATTATAAATTTTCCAGGTAG+TGG - chr5_4:64960923-64960942 None:intergenic 25.0%
!!! ATTTTCCTTGATTTGAATGA+AGG + chr5_4:64961165-64961184 Msa0864560:intron 25.0%
!!! TTTTCCTTGATTTGAATGAA+GGG + chr5_4:64961166-64961185 Msa0864560:intron 25.0%
! ATTGATACAAAGATGCAATT+TGG - chr5_4:64961618-64961637 None:intergenic 25.0%
! ATGCAATATCTTGTGAAATT+TGG + chr5_4:64961661-64961680 Msa0864560:CDS 25.0%
! GGCAAAATATATGATAATCA+AGG + chr5_4:64961682-64961701 Msa0864560:CDS 25.0%
! AGATTCTACTAAACATAGAT+AGG + chr5_4:64961825-64961844 Msa0864560:three_prime_UTR 25.0%
! GAAATACAAGAATTCTTCTA+AGG - chr5_4:64960366-64960385 None:intergenic 25.0%
!!! GTTTTGTTCTTATGATATGA+AGG + chr5_4:64960568-64960587 Msa0864560:intron 25.0%
! AGATAAACTTCTTAGCAATA+AGG + chr5_4:64960871-64960890 Msa0864560:CDS 25.0%
!! ATTATAAATTTTCCAGGTAG+TGG - chr5_4:64960923-64960942 None:intergenic 25.0%
!!! ATTTTCCTTGATTTGAATGA+AGG + chr5_4:64961165-64961184 Msa0864560:intron 25.0%
!!! TTTTCCTTGATTTGAATGAA+GGG + chr5_4:64961166-64961185 Msa0864560:intron 25.0%
! ATTGATACAAAGATGCAATT+TGG - chr5_4:64961618-64961637 None:intergenic 25.0%
! ATGCAATATCTTGTGAAATT+TGG + chr5_4:64961661-64961680 Msa0864560:CDS 25.0%
! GGCAAAATATATGATAATCA+AGG + chr5_4:64961682-64961701 Msa0864560:CDS 25.0%
! AGATTCTACTAAACATAGAT+AGG + chr5_4:64961825-64961844 Msa0864560:three_prime_UTR 25.0%
AGAAGGAAATAACATTGTCT+AGG - chr5_4:64960211-64960230 None:intergenic 30.0%
AGAAAGAAAGGAACAAAAGA+AGG - chr5_4:64960228-64960247 None:intergenic 30.0%
CTAACTATGAAAGATGCAAT+TGG - chr5_4:64960672-64960691 None:intergenic 30.0%
CAATTGCATCTTTCATAGTT+AGG + chr5_4:64960670-64960689 Msa0864560:CDS 30.0%
!!! CTTTTATTTCTACATCAGTG+TGG - chr5_4:64960853-64960872 None:intergenic 30.0%
TTTGAAAACTACATGTCCAA+AGG + chr5_4:64960961-64960980 Msa0864560:CDS 30.0%
!!! TGTTTGGTGTTGATTTTTGA+AGG + chr5_4:64961071-64961090 Msa0864560:intron 30.0%
! CATGTTTTCACACAATGTTA+AGG + chr5_4:64961375-64961394 Msa0864560:CDS 30.0%
GAAAGATTGAATTGACATGT+CGG - chr5_4:64961428-64961447 None:intergenic 30.0%
TGATATCTATGAGTTAAGAG+AGG + chr5_4:64961537-64961556 Msa0864560:CDS 30.0%
!!! AGTCTCTCAAGTACATTTTT+TGG - chr5_4:64961595-64961614 None:intergenic 30.0%
TCAAAGAAATTCTTGATGCT+TGG + chr5_4:64961758-64961777 Msa0864560:CDS 30.0%
CTAAACATAGATAGGTTGTT+TGG + chr5_4:64961833-64961852 Msa0864560:three_prime_UTR 30.0%
TAGATAGGTTGTTTGGTTAT+TGG + chr5_4:64961840-64961859 Msa0864560:three_prime_UTR 30.0%
TATAGGTAGTGCTCTTTATT+TGG + chr5_4:64961907-64961926 Msa0864560:three_prime_UTR 30.0%
AGAAGGAAATAACATTGTCT+AGG - chr5_4:64960211-64960230 None:intergenic 30.0%
AGAAAGAAAGGAACAAAAGA+AGG - chr5_4:64960228-64960247 None:intergenic 30.0%
CTAACTATGAAAGATGCAAT+TGG - chr5_4:64960672-64960691 None:intergenic 30.0%
CAATTGCATCTTTCATAGTT+AGG + chr5_4:64960670-64960689 Msa0864560:CDS 30.0%
!!! CTTTTATTTCTACATCAGTG+TGG - chr5_4:64960853-64960872 None:intergenic 30.0%
TTTGAAAACTACATGTCCAA+AGG + chr5_4:64960961-64960980 Msa0864560:CDS 30.0%
!!! TGTTTGGTGTTGATTTTTGA+AGG + chr5_4:64961071-64961090 Msa0864560:intron 30.0%
! CATGTTTTCACACAATGTTA+AGG + chr5_4:64961375-64961394 Msa0864560:CDS 30.0%
GAAAGATTGAATTGACATGT+CGG - chr5_4:64961428-64961447 None:intergenic 30.0%
TGATATCTATGAGTTAAGAG+AGG + chr5_4:64961537-64961556 Msa0864560:CDS 30.0%
!!! AGTCTCTCAAGTACATTTTT+TGG - chr5_4:64961595-64961614 None:intergenic 30.0%
TCAAAGAAATTCTTGATGCT+TGG + chr5_4:64961758-64961777 Msa0864560:CDS 30.0%
CTAAACATAGATAGGTTGTT+TGG + chr5_4:64961833-64961852 Msa0864560:three_prime_UTR 30.0%
TAGATAGGTTGTTTGGTTAT+TGG + chr5_4:64961840-64961859 Msa0864560:three_prime_UTR 30.0%
TATAGGTAGTGCTCTTTATT+TGG + chr5_4:64961907-64961926 Msa0864560:three_prime_UTR 30.0%
! GAAATAACATTGTCTAGGAC+AGG - chr5_4:64960206-64960225 None:intergenic 35.0%
TGTCAACTTGGAAACATGAT+AGG - chr5_4:64960277-64960296 None:intergenic 35.0%
! ATGTTTCCAAGTTGACAACT+TGG + chr5_4:64960280-64960299 Msa0864560:five_prime_UTR 35.0%
ATCCATTCAAAATAGCCATG+TGG + chr5_4:64960480-64960499 Msa0864560:CDS 35.0%
!! TTCCACATGGCTATTTTGAA+TGG - chr5_4:64960485-64960504 None:intergenic 35.0%
!! GATATGAAGGCATTTATCAG+TGG + chr5_4:64960581-64960600 Msa0864560:intron 35.0%
TTATCAGTGGAAAAGAACTG+TGG + chr5_4:64960594-64960613 Msa0864560:CDS 35.0%
!!! GTGATTGTTTTGGACAATTC+TGG - chr5_4:64960639-64960658 None:intergenic 35.0%
CTATGAAAGATGCAATTGGA+GGG - chr5_4:64960668-64960687 None:intergenic 35.0%
ACTATGAAAGATGCAATTGG+AGG - chr5_4:64960669-64960688 None:intergenic 35.0%
GTTAGGATTCTTAGTTATGC+TGG + chr5_4:64960687-64960706 Msa0864560:CDS 35.0%
AGAACTTGAATCACCTGATT+GGG - chr5_4:64960730-64960749 None:intergenic 35.0%
TAGAACTTGAATCACCTGAT+TGG - chr5_4:64960731-64960750 None:intergenic 35.0%
CAATAAGGATAGCTTTCTGT+GGG + chr5_4:64960886-64960905 Msa0864560:CDS 35.0%
! CGTTGATTGTTATGTCTGTA+TGG - chr5_4:64961288-64961307 None:intergenic 35.0%
TAGATTGGACCTATTACATG+CGG + chr5_4:64961504-64961523 Msa0864560:CDS 35.0%
! CATTAGAGTGCTAAACACAA+AGG - chr5_4:64961940-64961959 None:intergenic 35.0%
! GAAATAACATTGTCTAGGAC+AGG - chr5_4:64960206-64960225 None:intergenic 35.0%
TGTCAACTTGGAAACATGAT+AGG - chr5_4:64960277-64960296 None:intergenic 35.0%
! ATGTTTCCAAGTTGACAACT+TGG + chr5_4:64960280-64960299 Msa0864560:five_prime_UTR 35.0%
ATCCATTCAAAATAGCCATG+TGG + chr5_4:64960480-64960499 Msa0864560:CDS 35.0%
!! TTCCACATGGCTATTTTGAA+TGG - chr5_4:64960485-64960504 None:intergenic 35.0%
!! GATATGAAGGCATTTATCAG+TGG + chr5_4:64960581-64960600 Msa0864560:intron 35.0%
TTATCAGTGGAAAAGAACTG+TGG + chr5_4:64960594-64960613 Msa0864560:CDS 35.0%
!!! GTGATTGTTTTGGACAATTC+TGG - chr5_4:64960639-64960658 None:intergenic 35.0%
CTATGAAAGATGCAATTGGA+GGG - chr5_4:64960668-64960687 None:intergenic 35.0%
ACTATGAAAGATGCAATTGG+AGG - chr5_4:64960669-64960688 None:intergenic 35.0%
GTTAGGATTCTTAGTTATGC+TGG + chr5_4:64960687-64960706 Msa0864560:CDS 35.0%
AGAACTTGAATCACCTGATT+GGG - chr5_4:64960730-64960749 None:intergenic 35.0%
TAGAACTTGAATCACCTGAT+TGG - chr5_4:64960731-64960750 None:intergenic 35.0%
CAATAAGGATAGCTTTCTGT+GGG + chr5_4:64960886-64960905 Msa0864560:CDS 35.0%
! CGTTGATTGTTATGTCTGTA+TGG - chr5_4:64961288-64961307 None:intergenic 35.0%
TAGATTGGACCTATTACATG+CGG + chr5_4:64961504-64961523 Msa0864560:CDS 35.0%
! CATTAGAGTGCTAAACACAA+AGG - chr5_4:64961940-64961959 None:intergenic 35.0%
! ATAACATTGTCTAGGACAGG+AGG - chr5_4:64960203-64960222 None:intergenic 40.0%
CACAATCCAAGTTGTCAACT+TGG - chr5_4:64960289-64960308 None:intergenic 40.0%
AGTTGACAACTTGGATTGTG+AGG + chr5_4:64960289-64960308 Msa0864560:five_prime_UTR 40.0%
CCATTGAACTAGACGATTCA+TGG - chr5_4:64960420-64960439 None:intergenic 40.0%
! CCATGAATCGTCTAGTTCAA+TGG + chr5_4:64960417-64960436 Msa0864560:CDS 40.0%
TCAAAATAGCCATGTGGAAC+TGG + chr5_4:64960486-64960505 Msa0864560:CDS 40.0%
TTGACTTTACCAGTTCCACA+TGG - chr5_4:64960498-64960517 None:intergenic 40.0%
! AGGGTTGTGTGTGATTGTTT+TGG - chr5_4:64960649-64960668 None:intergenic 40.0%
AGCAAAATCTTGACCCAATC+AGG + chr5_4:64960714-64960733 Msa0864560:CDS 40.0%
GCAATAAGGATAGCTTTCTG+TGG + chr5_4:64960885-64960904 Msa0864560:CDS 40.0%
ATAGCTTTCTGTGGGAGATT+GGG + chr5_4:64960894-64960913 Msa0864560:CDS 40.0%
! TCCTTGATTTGAATGAAGGG+TGG + chr5_4:64961169-64961188 Msa0864560:intron 40.0%
! CCTTGATTTGAATGAAGGGT+GGG + chr5_4:64961170-64961189 Msa0864560:intron 40.0%
! CTTGATTTGAATGAAGGGTG+GGG + chr5_4:64961171-64961190 Msa0864560:intron 40.0%
TTCTCCTATCATTCGACCTA+GGG - chr5_4:64961260-64961279 None:intergenic 40.0%
TTTCTCCTATCATTCGACCT+AGG - chr5_4:64961261-64961280 None:intergenic 40.0%
AACAATCAACGTCAATTCCG+AGG + chr5_4:64961297-64961316 Msa0864560:CDS 40.0%
ACAATCAACGTCAATTCCGA+GGG + chr5_4:64961298-64961317 Msa0864560:CDS 40.0%
GAAAACATGCTGTGAAACAC+AGG - chr5_4:64961364-64961383 None:intergenic 40.0%
TGACATGTCGGTGATGTTTA+TGG - chr5_4:64961416-64961435 None:intergenic 40.0%
! ATAACATTGTCTAGGACAGG+AGG - chr5_4:64960203-64960222 None:intergenic 40.0%
CACAATCCAAGTTGTCAACT+TGG - chr5_4:64960289-64960308 None:intergenic 40.0%
AGTTGACAACTTGGATTGTG+AGG + chr5_4:64960289-64960308 Msa0864560:five_prime_UTR 40.0%
CCATTGAACTAGACGATTCA+TGG - chr5_4:64960420-64960439 None:intergenic 40.0%
! CCATGAATCGTCTAGTTCAA+TGG + chr5_4:64960417-64960436 Msa0864560:CDS 40.0%
TCAAAATAGCCATGTGGAAC+TGG + chr5_4:64960486-64960505 Msa0864560:CDS 40.0%
TTGACTTTACCAGTTCCACA+TGG - chr5_4:64960498-64960517 None:intergenic 40.0%
! AGGGTTGTGTGTGATTGTTT+TGG - chr5_4:64960649-64960668 None:intergenic 40.0%
AGCAAAATCTTGACCCAATC+AGG + chr5_4:64960714-64960733 Msa0864560:CDS 40.0%
GCAATAAGGATAGCTTTCTG+TGG + chr5_4:64960885-64960904 Msa0864560:CDS 40.0%
ATAGCTTTCTGTGGGAGATT+GGG + chr5_4:64960894-64960913 Msa0864560:CDS 40.0%
! TCCTTGATTTGAATGAAGGG+TGG + chr5_4:64961169-64961188 Msa0864560:intron 40.0%
! CCTTGATTTGAATGAAGGGT+GGG + chr5_4:64961170-64961189 Msa0864560:intron 40.0%
! CTTGATTTGAATGAAGGGTG+GGG + chr5_4:64961171-64961190 Msa0864560:intron 40.0%
TTCTCCTATCATTCGACCTA+GGG - chr5_4:64961260-64961279 None:intergenic 40.0%
TTTCTCCTATCATTCGACCT+AGG - chr5_4:64961261-64961280 None:intergenic 40.0%
AACAATCAACGTCAATTCCG+AGG + chr5_4:64961297-64961316 Msa0864560:CDS 40.0%
ACAATCAACGTCAATTCCGA+GGG + chr5_4:64961298-64961317 Msa0864560:CDS 40.0%
GAAAACATGCTGTGAAACAC+AGG - chr5_4:64961364-64961383 None:intergenic 40.0%
TGACATGTCGGTGATGTTTA+TGG - chr5_4:64961416-64961435 None:intergenic 40.0%
GATAGCTTTCTGTGGGAGAT+TGG + chr5_4:64960893-64960912 Msa0864560:CDS 45.0%
TAGCTTTCTGTGGGAGATTG+GGG + chr5_4:64960895-64960914 Msa0864560:CDS 45.0%
CCCACCCTTCATTCAAATCA+AGG - chr5_4:64961173-64961192 None:intergenic 45.0%
GCTTGCTCTATTTGGCCTAA+TGG + chr5_4:64961196-64961215 Msa0864560:CDS 45.0%
CTCGGCTGCATAAATCCATT+AGG - chr5_4:64961214-64961233 None:intergenic 45.0%
CATTCGACCTAGGGATTCAA+GGG - chr5_4:64961251-64961270 None:intergenic 45.0%
TCATTCGACCTAGGGATTCA+AGG - chr5_4:64961252-64961271 None:intergenic 45.0%
GAATCCCTAGGTCGAATGAT+AGG + chr5_4:64961253-64961272 Msa0864560:CDS 45.0%
ATGAGCTCTTATGCTTCCCT+CGG - chr5_4:64961317-64961336 None:intergenic 45.0%
GTGAAACACAGGTGATTGTG+AGG - chr5_4:64961353-64961372 None:intergenic 45.0%
ACTTGTCAGCCGCATGTAAT+AGG - chr5_4:64961516-64961535 None:intergenic 45.0%
GATAGCTTTCTGTGGGAGAT+TGG + chr5_4:64960893-64960912 Msa0864560:CDS 45.0%
TAGCTTTCTGTGGGAGATTG+GGG + chr5_4:64960895-64960914 Msa0864560:CDS 45.0%
CCCACCCTTCATTCAAATCA+AGG - chr5_4:64961173-64961192 None:intergenic 45.0%
GCTTGCTCTATTTGGCCTAA+TGG + chr5_4:64961196-64961215 Msa0864560:CDS 45.0%
CTCGGCTGCATAAATCCATT+AGG - chr5_4:64961214-64961233 None:intergenic 45.0%
CATTCGACCTAGGGATTCAA+GGG - chr5_4:64961251-64961270 None:intergenic 45.0%
TCATTCGACCTAGGGATTCA+AGG - chr5_4:64961252-64961271 None:intergenic 45.0%
GAATCCCTAGGTCGAATGAT+AGG + chr5_4:64961253-64961272 Msa0864560:CDS 45.0%
ATGAGCTCTTATGCTTCCCT+CGG - chr5_4:64961317-64961336 None:intergenic 45.0%
GTGAAACACAGGTGATTGTG+AGG - chr5_4:64961353-64961372 None:intergenic 45.0%
ACTTGTCAGCCGCATGTAAT+AGG - chr5_4:64961516-64961535 None:intergenic 45.0%
! GTGGGGAAGCTTGCTCTATT+TGG + chr5_4:64961188-64961207 Msa0864560:CDS 50.0%
! GTGGGGAAGCTTGCTCTATT+TGG + chr5_4:64961188-64961207 Msa0864560:CDS 50.0%
GAGATTGGGGTTCCACTACC+TGG + chr5_4:64960908-64960927 Msa0864560:CDS 55.0%
GCAGCCGAGACAAAATGCAG+CGG + chr5_4:64961225-64961244 Msa0864560:CDS 55.0%
! AGGGCCGCTGCATTTTGTCT+CGG - chr5_4:64961232-64961251 None:intergenic 55.0%
GAGATTGGGGTTCCACTACC+TGG + chr5_4:64960908-64960927 Msa0864560:CDS 55.0%
GCAGCCGAGACAAAATGCAG+CGG + chr5_4:64961225-64961244 Msa0864560:CDS 55.0%
! AGGGCCGCTGCATTTTGTCT+CGG - chr5_4:64961232-64961251 None:intergenic 55.0%
GCAGCGGCCCTTGAATCCCT+AGG + chr5_4:64961241-64961260 Msa0864560:CDS 65.0%
GCAGCGGCCCTTGAATCCCT+AGG + chr5_4:64961241-64961260 Msa0864560:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 64960185 64961984 64960185 ID=Msa0864560;Name=Msa0864560
chr5_4 mRNA 64960185 64961984 64960185 ID=Msa0864560-mRNA-1;Parent=Msa0864560;Name=Msa0864560-mRNA-1;_AED=0.14;_eAED=0.14;_QI=204|1|1|1|1|1|4|195|328
chr5_4 exon 64960185 64960507 64960185 ID=Msa0864560-mRNA-1:exon:20431;Parent=Msa0864560-mRNA-1
chr5_4 exon 64960590 64960735 64960590 ID=Msa0864560-mRNA-1:exon:20432;Parent=Msa0864560-mRNA-1
chr5_4 exon 64960864 64960982 64960864 ID=Msa0864560-mRNA-1:exon:20433;Parent=Msa0864560-mRNA-1
chr5_4 exon 64961187 64961984 64961187 ID=Msa0864560-mRNA-1:exon:20434;Parent=Msa0864560-mRNA-1
chr5_4 five_prime_UTR 64960185 64960388 64960185 ID=Msa0864560-mRNA-1:five_prime_utr;Parent=Msa0864560-mRNA-1
chr5_4 CDS 64960389 64960507 64960389 ID=Msa0864560-mRNA-1:cds;Parent=Msa0864560-mRNA-1
chr5_4 CDS 64960590 64960735 64960590 ID=Msa0864560-mRNA-1:cds;Parent=Msa0864560-mRNA-1
chr5_4 CDS 64960864 64960982 64960864 ID=Msa0864560-mRNA-1:cds;Parent=Msa0864560-mRNA-1
chr5_4 CDS 64961187 64961789 64961187 ID=Msa0864560-mRNA-1:cds;Parent=Msa0864560-mRNA-1
chr5_4 three_prime_UTR 64961790 64961984 64961790 ID=Msa0864560-mRNA-1:three_prime_utr;Parent=Msa0864560-mRNA-1
Gene Sequence

>Msa0864560

ATGAATGATTATGCATACAAAGTTGAAACCATGAATCGTCTAGTTCAATGGAAAATTCACAATTTATCAACATGCACTTATATCAAAGCTGATCCATTCAAAATAGCCATGTGGAACTGGCATTTATCAGTGGAAAAGAACTGTGGTTTACGTATTGAATTATATCCAGAATTGTCCAAAACAATCACACACAACCCTCCAATTGCATCTTTCATAGTTAGGATTCTTAGTTATGCTGGAAATAGCAAAATCTTGACCCAATCAGAAATAAAAGATAAACTTCTTAGCAATAAGGATAGCTTTCTGTGGGAGATTGGGGTTCCACTACCTGGAAAATTTATAATTGACATTGAGTTTCTTGATTTGAAAACTACATGTCCAAAGGGTGGGGAAGCTTGCTCTATTTGGCCTAATGGATTTATGCAGCCGAGACAAAATGCAGCGGCCCTTGAATCCCTAGGTCGAATGATAGGAGAAAGTATCCATACAGACATAACAATCAACGTCAATTCCGAGGGAAGCATAAGAGCTCATCGCGCTGTTCTTGCCTCACAATCACCTGTGTTTCACAGCATGTTTTCACACAATGTTAAGGAGACTGATTCGTCTACCATAAACATCACCGACATGTCAATTCAATCTTTCCAGACGTTTATAAATTTTCTTTATGGCAATGTCAATGATGAAGAATTTCTTATACATAGATTGGACCTATTACATGCGGCTGACAAGTATGATATCTATGAGTTAAGAGAGGCATGTGAAAAGAGTCTTCAAGAAGATATTGATCCAAAAAATGTACTTGAGAGACTCCAAATTGCATCTTTGTATCAATTAACAACGTTGAAGTTTAGTTGTATGCAATATCTTGTGAAATTTGGCAAAATATATGATAATCAAGGTGATTTAAGTTCATTCTTGCAAACTGCTGATAGAGATTTGATATGTGAAGTCTTCAAAGAAATTCTTGATGCTTGGAAAAAGTGA
Protein sequence

>Msa0864560

MNDYAYKVETMNRLVQWKIHNLSTCTYIKADPFKIAMWNWHLSVEKNCGLRIELYPELSKTITHNPPIASFIVRILSYAGNSKILTQSEIKDKLLSNKDSFLWEIGVPLPGKFIIDIEFLDLKTTCPKGGEACSIWPNGFMQPRQNAAALESLGRMIGESIHTDITINVNSEGSIRAHRAVLASQSPVFHSMFSHNVKETDSSTINITDMSIQSFQTFINFLYGNVNDEEFLIHRLDLLHAADKYDIYELREACEKSLQEDIDPKNVLERLQIASLYQLTTLKFSCMQYLVKFGKIYDNQGDLSSFLQTADRDLICEVFKEILDAWKK*