AlfalfaGEDB | Msa0864980

M. sativa cultivar zhongmu-4 / Msa0864980

KEGG: K13203
GO: -
PFAM: PF12874.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864980	XP_003618007.1	77.015	583	69	4	7	588	14	532	0.0	837

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864980	G7K4Z3	77.015	583	69	4	7	588	14	532	0.0	837

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TFs/TRs:

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Gene ID	Type	Classification
Msa0864980	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864980	MtrunA17_Chr5g0447971	77.015	583	69	4	7	588	14	532	0.0	837
Msa0864980	MtrunA17_Chr3g0086321	68.670	233	69	1	360	588	161	393	1.92e-101	313
Msa0864980	MtrunA17_Chr3g0086321	76.613	124	25	1	65	188	80	199	2.57e-56	194
Msa0864980	MtrunA17_Chr3g0086321	32.340	235	111	6	296	490	161	387	4.11e-16	80.5
Msa0864980	MtrunA17_Chr3g0086321	30.120	166	92	3	417	580	82	225	2.54e-12	68.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864980	AT5G61190.10	27.128	188	106	5	393	580	240	396	8.28e-12	68.9
Msa0864980	AT5G61190.1	27.128	188	106	5	393	580	240	396	9.61e-12	68.6
Msa0864980	AT5G61190.4	26.702	191	109	5	393	583	240	399	1.01e-11	68.6
Msa0864980	AT5G61190.2	26.702	191	109	5	393	583	240	399	1.01e-11	68.6
Msa0864980	AT5G61190.7	27.128	188	106	5	393	580	146	302	1.07e-11	68.6
Msa0864980	AT5G61190.3	27.128	188	106	5	393	580	146	302	1.07e-11	68.6
Msa0864980	AT5G61190.5	27.128	188	106	5	393	580	240	396	1.24e-11	68.6
Msa0864980	AT5G61190.6	27.128	188	106	5	393	580	240	396	1.27e-11	68.2
Msa0864980	AT5G61190.11	27.128	188	106	5	393	580	240	396	1.28e-11	68.2
Msa0864980	AT5G61190.9	27.128	188	106	5	393	580	240	396	1.38e-11	68.2

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Find 131 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGCTAAGAAAATCA+AGG	0.554241	5_4:+65563611	Msa0864980:CDS
AAACAGGTTGATAGTATACA+AGG	0.585879	5_4:+65561431	Msa0864980:CDS
AAACGTAAAGCTGTGGAAAG+AGG	0.559914	5_4:+65563879	Msa0864980:CDS
AAACTGTCAAATCCAAAAGT+CGG	0.636332	5_4:+65563735	Msa0864980:CDS
AACACAAGAAGAATTTGCAA+CGG	0.663613	5_4:+65561303	Msa0864980:CDS
AAGAAGGCAGGGTTAATCAC+TGG	0.566902	5_4:+65564008	Msa0864980:CDS
AAGCTTTGCTGACTTTGGTT+TGG	0.410273	5_4:-65561211	None:intergenic
AATGCCATCATTAGACTTCA+AGG	0.445716	5_4:-65563541	None:intergenic
AATTGAAGTGCCTATTGCGG+TGG	0.664606	5_4:-65563784	None:intergenic
ACAAAATCAACCTGCTTCTT+AGG	0.280074	5_4:-65561587	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACACAAGAAGAATTTGCAA+CGG	+	chr5_4:65561303-65561322	Msa0864980:CDS	30.0%
ATCCAAGGACAAACATTAAT+TGG	+	chr5_4:65561638-65561657	Msa0864980:CDS	30.0%
ACTAACTCAAGTAATATCCA+AGG	+	chr5_4:65562007-65562026	Msa0864980:CDS	30.0%
ACATATAACAAACATGTTGC+TGG	+	chr5_4:65562133-65562152	Msa0864980:CDS	30.0%
CATATAACAAACATGTTGCT+GGG	+	chr5_4:65562134-65562153	Msa0864980:CDS	30.0%
GACAGAACAAGAACATTAAA+AGG	+	chr5_4:65562359-65562378	Msa0864980:intron	30.0%
GTTTAGCTAATTAACTCTGT+AGG	+	chr5_4:65562436-65562455	Msa0864980:intron	30.0%
GAAAATAAGAGAATCTTGGT+GGG	-	chr5_4:65562597-65562616	None:intergenic	30.0%
TGAAAATAAGAGAATCTTGG+TGG	-	chr5_4:65562598-65562617	None:intergenic	30.0%
GGAAAAAGTAACTGGAAATA+AGG	+	chr5_4:65562665-65562684	Msa0864980:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	65560906	65564250	65560906	ID=Msa0864980;Name=Msa0864980
chr5_4	mRNA	65560906	65564250	65560906	ID=Msa0864980-mRNA-1;Parent=Msa0864980;Name=Msa0864980-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0\|1\|0.5\|1\|1\|1\|2\|216\|588
chr5_4	exon	65560906	65562174	65560906	ID=Msa0864980-mRNA-1:exon:20661;Parent=Msa0864980-mRNA-1
chr5_4	exon	65563537	65564250	65563537	ID=Msa0864980-mRNA-1:exon:20662;Parent=Msa0864980-mRNA-1
chr5_4	CDS	65560906	65562174	65560906	ID=Msa0864980-mRNA-1:cds;Parent=Msa0864980-mRNA-1
chr5_4	CDS	65563537	65564034	65563537	ID=Msa0864980-mRNA-1:cds;Parent=Msa0864980-mRNA-1
chr5_4	three_prime_UTR	65564035	65564250	65564035	ID=Msa0864980-mRNA-1:three_prime_utr;Parent=Msa0864980-mRNA-1

Gene Sequence

>Msa0864980

ATGCTTCTAATATTGCAGGCTGTTGGTTCATCACACGAGACTTCAGCAGTGCCTGCAAGTTTGCCTTCTGTTCAGAATGGTATTCCAATGCTTGTAATTTTGCAGTCTGCTGGTTCATCACACGAGACTTCAGCAGCGCCTGCAAGTTTGCCTTCTGCGCAGAATGGTATGCCAATGCTTCTAATATTGCCGGCTGTTGGTTCATCACACCAGACTTCAGCAGCGCCTGCAAGTTTGCCTTCTGCACGTAATGGTTCCTGGCAACTTCAAATTTATGGCAATCAGACTACAATAATGTTGCCTAACCAAACCAAAGTCAGCAAAGCTTTCAGATGTGAAGTGTGTGAAATTGATGTCAATAGCCAAGTCTCTTTTGAAAAGCACATAGCTGGAAAGAAACACAAGAAGAATTTGCAACGGCAGACTAATCCTACAATTGCATCTCATGCGAATTTACAAACTGACACAAGTAGTATCCAAGGTCAAGCGTTAATTGGTCCTGTAGGCGAGCAATCAGAGCCTAAGAAACAGGTTGATAGTATACAAGGACAAGCTTTAATCTGTCCTGTAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCCGTTGAGAATGTACAAACCGACACAAGAAGTATTGAAGGACAAGCGTTAATTGTTGCTGTACCCAAACAATCAGAGCCTAAGAAGCAGGTTGATTTTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAACATTAATTGGTCCTGTAGCCGGGCAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAGGATCCATGGACAAGCGTTAATCGGTCCTGTAGCCGAACAATCAGAGCCTAAAAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAGCATTAATTGGTCTTGTAGCCAAACAATCAGAGCCTAAGAAACAGGTTGGTTCTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAATCACAAGCCTTAATTGGTCCTTTAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACTAACTCAAGTAATATCCAAGGACAAGCGTTAATTGGTCCTATAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGGTTTGCTCAACATGCAATGTTGTATGCGTTGGTCAAGATACATATAACAAACATGTTGCTGGGAGAAAGCATGCTGCTAAGGTTGCCTTGAAGTCTAATGATGGCATTGGTCCATCTATTGCCGAACTCAAGCGTAACGGTGATGCTCTAATTGAAAAAGCTGCTAAGAAAATCAAGGTTGCTGAATCCGTATGGTGTGAATTTTGCAAGATTAATTGTAACAGCAAGTATAGCTACACTGCACACTTGTCCGGTAAGAAACACTTGAAGAACATGGAGAAACTGTCAAATCCAAAAGTCGGTGTTGGCAGCGGTGCCACTACCACCACCACCGCAATAGGCACTTCAATTGGGCCACAGGAAAAGCCAGATTCAGATAAACCGAAAACAAAGGAGGTATCAGTACTTGATATCGAGACAGAGAAACGTAAAGCTGTGGAAAGAGGAGCTGCAGTCGATGATATTAAATTGTGCACTTTATGCAATGTGGTGTGTAATAGCCAGGCAAATTTAAATAATCACCTTAGTGACCATAATCATGCTGCTATGGTGAAGAAGGCAGGGTTAATCACTGGTTGA

Protein sequence

>Msa0864980

MLLILQAVGSSHETSAVPASLPSVQNGIPMLVILQSAGSSHETSAAPASLPSAQNGMPMLLILPAVGSSHQTSAAPASLPSARNGSWQLQIYGNQTTIMLPNQTKVSKAFRCEVCEIDVNSQVSFEKHIAGKKHKKNLQRQTNPTIASHANLQTDTSSIQGQALIGPVGEQSEPKKQVDSIQGQALICPVAEQSEPKKQVDSVENVQTDTRSIEGQALIVAVPKQSEPKKQVDFVKNVQTDTSSIQGQTLIGPVAGQSEPKKQVDSVKNVQTDTSRIHGQALIGPVAEQSEPKKQVDSVKNVQTDTSSIQGQALIGLVAKQSEPKKQVGSVKNVQTDTSSIQSQALIGPLAEQSEPKKQVDSVKNVQTNSSNIQGQALIGPIAEQSEPKKQVDSVKVCSTCNVVCVGQDTYNKHVAGRKHAAKVALKSNDGIGPSIAELKRNGDALIEKAAKKIKVAESVWCEFCKINCNSKYSYTAHLSGKKHLKNMEKLSNPKVGVGSGATTTTTAIGTSIGPQEKPDSDKPKTKEVSVLDIETEKRKAVERGAAVDDIKLCTLCNVVCNSQANLNNHLSDHNHAAMVKKAGLITG*