Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0868090 | AES97588.1 | 93.038 | 158 | 11 | 0 | 1 | 158 | 1 | 158 | 4.11e-94 | 279 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0868090 | G7K1J4 | 93.038 | 158 | 11 | 0 | 1 | 158 | 1 | 158 | 1.96e-94 | 279 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0868090 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0868090 | MtrunA17_Chr5g0423491 | 93.038 | 158 | 11 | 0 | 1 | 158 | 1 | 158 | 3.78e-98 | 279 |
| Msa0868090 | MtrunA17_Chr1g0166461 | 58.046 | 174 | 50 | 5 | 1 | 158 | 1 | 167 | 1.32e-58 | 179 |
| Msa0868090 | MtrunA17_Chr1g0170601 | 47.953 | 171 | 58 | 7 | 1 | 158 | 1 | 153 | 2.89e-34 | 117 |
| Msa0868090 | MtrunA17_Chr1g0187511 | 35.032 | 157 | 78 | 6 | 11 | 158 | 4 | 145 | 4.73e-14 | 65.5 |
| Msa0868090 | MtrunA17_Chr1g0187541 | 32.624 | 141 | 80 | 4 | 11 | 144 | 6 | 138 | 8.08e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATTGATTTCATTGATT+TGG | 0.224195 | 6_1:+1556820 | Msa0868090:CDS |
| CATTTGAAGATTCTCCTTCT+AGG | 0.234818 | 6_1:-1556754 | None:intergenic |
| TGAGGAGGAAATTGTCCTTT+AGG | 0.244142 | 6_1:-1556593 | None:intergenic |
| AACCAAAACCAACGGTTATT+CGG | 0.300666 | 6_1:+1556648 | Msa0868090:CDS |
| GAGGAGGAAATTGTCCTTTA+GGG | 0.320849 | 6_1:-1556592 | None:intergenic |
| AAATCCAATGGATTAACATC+TGG | 0.363954 | 6_1:-1556863 | None:intergenic |
| TGCTTCGATTGGTAAGCATT+TGG | 0.404776 | 6_1:-1556731 | None:intergenic |
| CTGGTGAGTTTAGTAATATT+AGG | 0.423811 | 6_1:+1556429 | Msa0868090:CDS |
| TGAATAAAGCTTTCTGATTG+TGG | 0.433760 | 6_1:-1556555 | None:intergenic |
| TACCAATCGAAGCACCTAGA+AGG | 0.448280 | 6_1:+1556740 | Msa0868090:CDS |
| CTCCTTCTAGGTGCTTCGAT+TGG | 0.488901 | 6_1:-1556742 | None:intergenic |
| GAAGGAGAATCTTCAAATGA+TGG | 0.491518 | 6_1:+1556758 | Msa0868090:CDS |
| TAACCCAGATGTTAATCCAT+TGG | 0.496678 | 6_1:+1556859 | Msa0868090:CDS |
| AATCCAATGGATTAACATCT+GGG | 0.500492 | 6_1:-1556862 | None:intergenic |
| ATCTTCAAATGATGGTACTA+AGG | 0.506473 | 6_1:+1556766 | Msa0868090:CDS |
| GGCTGAGGCCGAATAACCGT+TGG | 0.511549 | 6_1:-1556656 | None:intergenic |
| AAAGGGTTTCTGCTTGGCTG+AGG | 0.532206 | 6_1:-1556671 | None:intergenic |
| TTCGATTGGTAAGCATTTGG+TGG | 0.535435 | 6_1:-1556728 | None:intergenic |
| CATGATGTGTCAAACTTGGT+AGG | 0.541015 | 6_1:+1556452 | Msa0868090:CDS |
| TGCAACATCATCACCATGGC+TGG | 0.552771 | 6_1:+1556410 | None:intergenic |
| AATCAGAAAGCTTTATTCAA+CGG | 0.559288 | 6_1:+1556559 | Msa0868090:CDS |
| TAGGCATGATGTGTCAAACT+TGG | 0.564079 | 6_1:+1556448 | Msa0868090:CDS |
| AGCTTAAGTGATAAATCCAA+TGG | 0.575565 | 6_1:-1556875 | None:intergenic |
| CAATGTTTGCCACCACATGA+TGG | 0.576640 | 6_1:+1556614 | Msa0868090:CDS |
| ACGGAGCGTATCAACCCTAA+AGG | 0.578711 | 6_1:+1556578 | Msa0868090:CDS |
| AACTCACATCGAGTCTCACA+TGG | 0.586787 | 6_1:+1556520 | Msa0868090:CDS |
| ATTACTAAACTCACCAGCCA+TGG | 0.594645 | 6_1:-1556423 | None:intergenic |
| TCATGTGGTGGCAAACATTG+AGG | 0.597472 | 6_1:-1556611 | None:intergenic |
| GCATTTGCCGATAACCAACA+TGG | 0.630189 | 6_1:-1556700 | None:intergenic |
| ATGATGTGTCAAACTTGGTA+GGG | 0.645237 | 6_1:+1556453 | Msa0868090:CDS |
| TGTGGTGGCAAACATTGAGG+AGG | 0.673338 | 6_1:-1556608 | None:intergenic |
| GTCTCACATGGAGTACGAAG+AGG | 0.675983 | 6_1:+1556532 | Msa0868090:CDS |
| AAAATCTTGCCATCATGTGG+TGG | 0.685328 | 6_1:-1556623 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGAAATCAATTTTCTTGAT+AGG | - | chr6_1:1556815-1556834 | None:intergenic | 20.0% |
| !!! | GAAAATTGATTTCATTGATT+TGG | + | chr6_1:1556820-1556839 | Msa0868090:CDS | 20.0% |
| !!! | AATCAGAAAGCTTTATTCAA+CGG | + | chr6_1:1556559-1556578 | Msa0868090:CDS | 25.0% |
| CTGGTGAGTTTAGTAATATT+AGG | + | chr6_1:1556429-1556448 | Msa0868090:CDS | 30.0% | |
| TTTGAGTGTTTGTGAAAAAG+CGG | - | chr6_1:1556493-1556512 | None:intergenic | 30.0% | |
| TGAATAAAGCTTTCTGATTG+TGG | - | chr6_1:1556558-1556577 | None:intergenic | 30.0% | |
| !! | GATTTTTCAACCAAAACCAA+CGG | + | chr6_1:1556640-1556659 | Msa0868090:CDS | 30.0% |
| ATCTTCAAATGATGGTACTA+AGG | + | chr6_1:1556766-1556785 | Msa0868090:CDS | 30.0% | |
| !! | AATCCAATGGATTAACATCT+GGG | - | chr6_1:1556865-1556884 | None:intergenic | 30.0% |
| !! | AAATCCAATGGATTAACATC+TGG | - | chr6_1:1556866-1556885 | None:intergenic | 30.0% |
| AGCTTAAGTGATAAATCCAA+TGG | - | chr6_1:1556878-1556897 | None:intergenic | 30.0% | |
| ATGATGTGTCAAACTTGGTA+GGG | + | chr6_1:1556453-1556472 | Msa0868090:CDS | 35.0% | |
| TGAAAAATCTTGCCATCATG+TGG | - | chr6_1:1556629-1556648 | None:intergenic | 35.0% | |
| AACCAAAACCAACGGTTATT+CGG | + | chr6_1:1556648-1556667 | Msa0868090:CDS | 35.0% | |
| CATTTGAAGATTCTCCTTCT+AGG | - | chr6_1:1556757-1556776 | None:intergenic | 35.0% | |
| GAAGGAGAATCTTCAAATGA+TGG | + | chr6_1:1556758-1556777 | Msa0868090:CDS | 35.0% | |
| TAACCCAGATGTTAATCCAT+TGG | + | chr6_1:1556859-1556878 | Msa0868090:CDS | 35.0% | |
| TAGGCATGATGTGTCAAACT+TGG | + | chr6_1:1556448-1556467 | Msa0868090:CDS | 40.0% | |
| CATGATGTGTCAAACTTGGT+AGG | + | chr6_1:1556452-1556471 | Msa0868090:CDS | 40.0% | |
| GAGGAGGAAATTGTCCTTTA+GGG | - | chr6_1:1556595-1556614 | None:intergenic | 40.0% | |
| TGAGGAGGAAATTGTCCTTT+AGG | - | chr6_1:1556596-1556615 | None:intergenic | 40.0% | |
| AAAATCTTGCCATCATGTGG+TGG | - | chr6_1:1556626-1556645 | None:intergenic | 40.0% | |
| !!! | CCAAGCAGAAACCCTTTTTT+CGG | + | chr6_1:1556677-1556696 | Msa0868090:CDS | 40.0% |
| AACCAACATGGCCGAAAAAA+GGG | - | chr6_1:1556691-1556710 | None:intergenic | 40.0% | |
| TAACCAACATGGCCGAAAAA+AGG | - | chr6_1:1556692-1556711 | None:intergenic | 40.0% | |
| !! | TTTTCGGCCATGTTGGTTAT+CGG | + | chr6_1:1556693-1556712 | Msa0868090:CDS | 40.0% |
| TTCGATTGGTAAGCATTTGG+TGG | - | chr6_1:1556731-1556750 | None:intergenic | 40.0% | |
| TGCTTCGATTGGTAAGCATT+TGG | - | chr6_1:1556734-1556753 | None:intergenic | 40.0% | |
| AACTCACATCGAGTCTCACA+TGG | + | chr6_1:1556520-1556539 | Msa0868090:CDS | 45.0% | |
| TCATGTGGTGGCAAACATTG+AGG | - | chr6_1:1556614-1556633 | None:intergenic | 45.0% | |
| CAATGTTTGCCACCACATGA+TGG | + | chr6_1:1556614-1556633 | Msa0868090:CDS | 45.0% | |
| CCGAAAAAAGGGTTTCTGCT+TGG | - | chr6_1:1556680-1556699 | None:intergenic | 45.0% | |
| !!! | AACCCTTTTTTCGGCCATGT+TGG | + | chr6_1:1556686-1556705 | Msa0868090:CDS | 45.0% |
| GCATTTGCCGATAACCAACA+TGG | - | chr6_1:1556703-1556722 | None:intergenic | 45.0% | |
| ! | TACCAATCGAAGCACCTAGA+AGG | + | chr6_1:1556740-1556759 | Msa0868090:CDS | 45.0% |
| GTCTCACATGGAGTACGAAG+AGG | + | chr6_1:1556532-1556551 | Msa0868090:CDS | 50.0% | |
| ACGGAGCGTATCAACCCTAA+AGG | + | chr6_1:1556578-1556597 | Msa0868090:CDS | 50.0% | |
| TGTGGTGGCAAACATTGAGG+AGG | - | chr6_1:1556611-1556630 | None:intergenic | 50.0% | |
| !!! | GGCCGAATAACCGTTGGTTT+TGG | - | chr6_1:1556653-1556672 | None:intergenic | 50.0% |
| AAAGGGTTTCTGCTTGGCTG+AGG | - | chr6_1:1556674-1556693 | None:intergenic | 50.0% | |
| CTCCTTCTAGGTGCTTCGAT+TGG | - | chr6_1:1556745-1556764 | None:intergenic | 50.0% | |
| GGCTGAGGCCGAATAACCGT+TGG | - | chr6_1:1556659-1556678 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 1556425 | 1556901 | 1556425 | ID=Msa0868090;Name=Msa0868090 |
| chr6_1 | mRNA | 1556425 | 1556901 | 1556425 | ID=Msa0868090-mRNA-1;Parent=Msa0868090;Name=Msa0868090-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|158 |
| chr6_1 | exon | 1556425 | 1556901 | 1556425 | ID=Msa0868090-mRNA-1:exon:418;Parent=Msa0868090-mRNA-1 |
| chr6_1 | CDS | 1556425 | 1556901 | 1556425 | ID=Msa0868090-mRNA-1:cds;Parent=Msa0868090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
