AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0868470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868470 MtrunA17_Chr6g0449311 80.606 165 11 1 37 201 1 144 5.39e-78 231
Msa0868470 MtrunA17_Chr6g0452661 55.959 193 69 4 1 190 1 180 1.03e-63 199
Msa0868470 MtrunA17_Chr5g0439111 64.865 185 50 6 1 179 1 176 1.97e-62 195
Msa0868470 MtrunA17_Chr4g0055441 61.151 139 40 3 10 145 3 130 4.09e-51 164
Msa0868470 MtrunA17_Chr6g0452691 54.730 148 54 3 1 142 1 141 6.26e-49 159
Msa0868470 MtrunA17_Chr6g0452671 55.634 142 56 3 1 140 1 137 3.08e-45 150
Msa0868470 MtrunA17_Chr4g0059901 50.467 107 52 1 8 114 9 114 9.89e-31 114
Msa0868470 MtrunA17_Chr8g0342351 50.467 107 52 1 8 114 11 116 1.83e-28 108
Msa0868470 MtrunA17_Chr4g0058651 38.060 134 71 2 7 140 9 130 6.29e-25 97.4
Msa0868470 MtrunA17_Chr7g0227511 34.161 161 92 4 7 162 9 160 6.46e-25 97.1
Msa0868470 MtrunA17_Chr2g0330201 43.119 109 61 1 8 116 18 125 1.45e-23 96.7
Msa0868470 MtrunA17_Chr3g0097221 42.857 112 63 1 7 118 5 115 2.69e-23 95.5
Msa0868470 MtrunA17_Chr8g0353981 48.193 83 42 1 7 89 5 86 2.79e-23 94.4
Msa0868470 MtrunA17_Chr5g0402611 42.857 105 59 1 7 111 6 109 5.68e-23 95.1
Msa0868470 MtrunA17_Chr3g0117121 42.857 98 55 1 7 104 8 104 7.91e-23 92.0
Msa0868470 MtrunA17_Chr3g0115431 42.157 102 58 1 10 111 10 110 2.71e-22 91.3
Msa0868470 MtrunA17_Chr6g0449641 40.816 98 57 1 7 104 7 103 9.89e-22 87.8
Msa0868470 MtrunA17_Chr5g0444351 31.469 143 94 2 10 152 6 144 1.10e-21 87.8
Msa0868470 MtrunA17_Chr4g0043421 36.641 131 81 2 10 140 16 144 2.69e-21 87.4
Msa0868470 MtrunA17_Chr7g0225111 37.273 110 68 1 10 119 6 114 1.47e-20 84.0
Msa0868470 MtrunA17_Chr2g0311601 35.833 120 70 2 10 129 14 126 1.70e-20 85.1
Msa0868470 MtrunA17_Chr6g0449651 39.796 98 58 1 7 104 65 161 3.22e-20 85.5
Msa0868470 MtrunA17_Chr5g0437401 35.780 109 69 1 5 113 7 114 7.46e-20 84.0
Msa0868470 MtrunA17_Chr5g0437571 36.207 116 72 2 7 122 4 117 7.69e-20 83.6
Msa0868470 MtrunA17_Chr4g0030001 39.655 116 67 3 10 124 25 138 9.96e-20 84.0
Msa0868470 MtrunA17_Chr5g0438091 32.331 133 88 2 10 142 40 170 2.05e-19 83.2
Msa0868470 MtrunA17_Chr8g0374591 38.462 104 63 1 8 111 7 109 3.42e-19 80.9
Msa0868470 MtrunA17_Chr3g0106101 36.036 111 69 2 10 120 8 116 6.52e-19 81.3
Msa0868470 MtrunA17_Chr8g0374601 38.462 104 63 1 8 111 25 127 4.95e-18 81.3
Msa0868470 MtrunA17_Chr3g0113891 36.538 104 65 1 5 108 10 112 6.50e-18 79.0
Msa0868470 MtrunA17_Chr5g0432451 32.576 132 84 2 10 141 44 170 1.69e-17 78.6
Msa0868470 MtrunA17_Chr1g0200201 35.606 132 73 4 10 132 73 201 2.06e-17 78.6
Msa0868470 MtrunA17_Chr3g0114061 38.462 104 62 2 10 113 9 110 2.96e-17 76.3
Msa0868470 MtrunA17_Chr4g0040831 31.783 129 79 3 8 132 14 137 6.26e-17 76.6
Msa0868470 MtrunA17_Chr3g0129071 35.714 98 62 1 10 107 48 144 8.22e-17 77.0
Msa0868470 MtrunA17_Chr7g0260971 38.835 103 61 2 10 111 15 116 3.91e-16 74.7
Msa0868470 MtrunA17_Chr8g0351721 34.259 108 69 2 5 111 14 120 2.22e-14 70.1
Msa0868470 MtrunA17_Chr6g0460951 33.333 102 66 2 10 110 41 141 1.68e-13 67.8
Msa0868470 MtrunA17_Chr7g0245871 35.354 99 62 2 10 107 28 125 1.78e-13 67.8
Msa0868470 MtrunA17_Chr8g0351701 33.010 103 67 2 10 111 24 125 2.88e-13 67.0
Msa0868470 MtrunA17_Chr4g0046201 36.364 88 55 1 9 96 6 92 1.89e-12 65.5
Msa0868470 MtrunA17_Chr6g0460941 35.227 88 55 2 10 96 8 94 2.35e-12 61.6
Msa0868470 MtrunA17_Chr8g0374581 34.615 104 66 2 8 110 7 109 7.85e-12 63.2
Msa0868470 MtrunA17_Chr2g0326041 50.000 48 24 0 10 57 34 81 7.13e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0868470 AT3G47870.1 54.808 104 46 1 9 112 36 138 4.34e-33 122
Msa0868470 AT3G13850.1 42.568 148 80 3 8 152 35 180 2.09e-28 108
Msa0868470 AT5G63090.4 32.609 184 116 4 7 188 9 186 4.36e-23 92.8
Msa0868470 AT5G63090.3 32.609 184 116 4 7 188 9 186 4.36e-23 92.8
Msa0868470 AT5G63090.2 32.609 184 116 4 7 188 9 186 4.36e-23 92.8
Msa0868470 AT5G63090.1 32.609 184 116 4 7 188 9 186 4.36e-23 92.8
Msa0868470 AT3G27650.1 41.000 100 58 1 7 106 37 135 1.58e-22 90.9
Msa0868470 AT1G65620.1 45.783 83 44 1 7 89 7 88 2.45e-21 88.6
Msa0868470 AT1G65620.4 45.783 83 44 1 7 89 7 88 2.45e-21 88.6
Msa0868470 AT1G65620.3 45.783 83 44 1 7 89 7 88 2.45e-21 88.6
Msa0868470 AT1G65620.5 45.783 83 44 1 7 89 7 88 2.45e-21 88.6
Msa0868470 AT1G65620.2 45.783 83 44 1 7 89 7 88 2.45e-21 88.6
Msa0868470 AT5G66870.1 40.196 102 60 1 10 111 8 108 8.52e-21 89.7
Msa0868470 AT3G26660.1 38.261 115 68 2 8 120 4 117 9.37e-21 85.1
Msa0868470 AT1G31320.1 43.158 95 50 2 5 96 6 99 3.30e-20 85.1
Msa0868470 AT1G72980.1 40.179 112 65 2 9 119 13 123 2.40e-19 83.6
Msa0868470 AT3G26620.1 37.500 112 67 2 8 117 4 114 2.62e-19 81.3
Msa0868470 AT1G07900.1 41.489 94 54 1 10 103 34 126 1.41e-18 81.3
Msa0868470 AT2G28500.1 37.391 115 70 2 10 124 56 168 1.54e-17 79.3
Msa0868470 AT2G30130.1 38.462 104 62 2 10 113 9 110 1.93e-17 78.2
Msa0868470 AT2G23660.2 39.604 101 60 1 10 110 6 105 3.19e-17 79.7
Msa0868470 AT2G23660.3 39.604 101 60 1 10 110 6 105 3.19e-17 79.7
Msa0868470 AT2G23660.1 39.604 101 60 1 10 110 6 105 3.19e-17 79.7
Msa0868470 AT2G30340.2 36.735 98 61 1 10 107 54 150 6.79e-17 78.2
Msa0868470 AT2G30340.1 36.735 98 61 1 10 107 53 149 8.02e-17 77.8
Msa0868470 AT3G11090.1 35.354 99 63 1 5 103 7 104 8.52e-17 75.9
Msa0868470 AT2G40470.2 33.094 139 83 4 10 148 21 149 1.59e-16 75.9
Msa0868470 AT2G40470.1 33.094 139 83 4 10 148 46 174 2.16e-16 75.9
Msa0868470 AT1G16530.1 35.043 117 64 4 4 109 6 121 3.40e-16 73.9
Msa0868470 AT5G15060.1 44.595 74 40 1 10 82 18 91 1.78e-15 71.6
Msa0868470 AT3G50510.1 37.143 105 64 2 10 113 13 116 8.56e-15 71.2
Msa0868470 AT3G50510.2 37.143 105 64 2 10 113 13 116 8.56e-15 71.2
Msa0868470 AT4G00210.1 33.333 138 84 4 10 145 12 143 4.77e-14 69.3
Msa0868470 AT2G19820.1 33.333 99 63 1 1 96 1 99 1.06e-13 66.2
Msa0868470 AT1G06280.1 36.170 94 55 3 8 101 23 111 3.33e-13 67.0
Msa0868470 AT4G00220.1 33.333 105 68 2 12 115 20 123 3.84e-13 67.0
Msa0868470 AT2G42430.1 28.205 156 104 3 10 158 16 170 4.97e-13 67.0
Msa0868470 AT2G45420.1 32.381 105 69 2 12 115 40 143 1.63e-12 65.9
Msa0868470 AT3G03760.1 34.314 102 65 2 10 110 52 152 5.94e-12 64.3
Msa0868470 AT5G35900.1 28.155 103 73 1 9 111 5 106 1.73e-11 62.0

Find 30 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCAACATTGCTAGATAT+TGG 0.233878 6_1:+1977985 Msa0868460:intron
TCACCAACATTGTTAAATAT+TGG 0.234385 6_1:+1978261 Msa0868460:intron
TCACTATTGTGATAATAATA+AGG 0.243500 6_1:+1978234 Msa0868460:intron
CTTTCTTCTTTGATATTTGC+AGG 0.243555 6_1:+1978644 Msa0868460:intron
GTTAAGAATACTCAAGTTCT+TGG 0.244885 6_1:-1978144 Msa0868470:CDS
TGGATGTCTTGGTCTTATTA+TGG 0.281895 6_1:-1978430 Msa0868470:CDS
GGTTTATCGGCTGGAAAATA+AGG 0.296290 6_1:+1978597 Msa0868460:intron
GCCAATGCTCATCGTTTGTT+TGG 0.347008 6_1:-1978564 Msa0868470:CDS
TAAGGAGCTAAAGGGAAATC+TGG 0.373984 6_1:+1978615 Msa0868460:intron
ACCACTAGTTCTCAAATTCC+AGG 0.434386 6_1:-1978015 Msa0868470:CDS
ATTCCAATATTTAACAATGT+TGG 0.448330 6_1:-1978264 Msa0868470:CDS
TCTTCTACATTTCTCTTCAT+CGG 0.464845 6_1:-1978319 Msa0868470:CDS
GGAATTGAAGAACTAGTAGA+AGG 0.471090 6_1:+1978282 Msa0868460:intron
TTCCCAAGTCATGGATGTCT+TGG 0.482571 6_1:-1978441 Msa0868470:CDS
GCTGGAAAATAAGGAGCTAA+AGG 0.488184 6_1:+1978606 Msa0868460:intron
CTGGAAAATAAGGAGCTAAA+GGG 0.491954 6_1:+1978607 Msa0868460:intron
TCTTGATATGATAAAGTATG+AGG 0.492253 6_1:-1978118 Msa0868470:CDS
CTAGATATTGGATTAATACC+TGG 0.493908 6_1:+1977997 Msa0868460:intron
GAATGATCCATTGAAAAGTA+AGG 0.496518 6_1:+1978048 Msa0868460:intron
AATCCAATATCTAGCAATGT+TGG 0.514330 6_1:-1977988 Msa0868470:CDS
AGACCAAGACATCCATGACT+TGG 0.541471 6_1:+1978438 Msa0868460:intron
ACCAAACAAACGATGAGCAT+TGG 0.553402 6_1:+1978563 Msa0868460:intron
CGTGCAAGATTCCCAAGTCA+TGG 0.565788 6_1:-1978450 Msa0868470:CDS
GTAAATTTGTTAACATCGTG+AGG 0.570877 6_1:+1977954 Msa0868460:intron
ACCTGGAATTTGAGAACTAG+TGG 0.587717 6_1:+1978014 Msa0868460:intron
GACCAAGACATCCATGACTT+GGG 0.599785 6_1:+1978439 Msa0868460:intron
TTTGTTTGGTATTTCCAACA+TGG 0.612102 6_1:-1978550 Msa0868470:CDS
TGAGTATTCTTAACATCACG+AGG 0.634892 6_1:+1978153 Msa0868460:intron
GGAATTTGAGAACTAGTGGT+AGG 0.664884 6_1:+1978018 Msa0868460:intron
GAGTATTCTTAACATCACGA+GGG 0.718609 6_1:+1978154 Msa0868460:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATCGATTATTTTTGAAT+TGG - chr6_1:1978163-1978182 Msa0868470:CDS 20.0%
!! ATTCCAATATTTAACAATGT+TGG - chr6_1:1978377-1978396 Msa0868470:CDS 20.0%
!! TCACTATTGTGATAATAATA+AGG + chr6_1:1978410-1978429 Msa0868460:intron 20.0%
! TCACCAACATTGTTAAATAT+TGG + chr6_1:1978383-1978402 Msa0868460:intron 25.0%
! TCTTGATATGATAAAGTATG+AGG - chr6_1:1978523-1978542 Msa0868470:CDS 25.0%
!!! TTTTCTACCTTACTTTTCAA+TGG - chr6_1:1978586-1978605 Msa0868470:CDS 25.0%
GGATATTCACAGAAAAAACA+AGG - chr6_1:1977965-1977984 Msa0868470:CDS 30.0%
CTTTCTTCTTTGATATTTGC+AGG + chr6_1:1978000-1978019 Msa0868460:intron 30.0%
TTTGTTTGGTATTTCCAACA+TGG - chr6_1:1978091-1978110 Msa0868470:CDS 30.0%
TCTTCTACATTTCTCTTCAT+CGG - chr6_1:1978322-1978341 Msa0868470:CDS 30.0%
GTTAAGAATACTCAAGTTCT+TGG - chr6_1:1978497-1978516 Msa0868470:CDS 30.0%
GAATGATCCATTGAAAAGTA+AGG + chr6_1:1978596-1978615 Msa0868460:intron 30.0%
CTAGATATTGGATTAATACC+TGG + chr6_1:1978647-1978666 Msa0868460:intron 30.0%
AATCCAATATCTAGCAATGT+TGG - chr6_1:1978653-1978672 Msa0868470:CDS 30.0%
TTACCAACATTGCTAGATAT+TGG + chr6_1:1978659-1978678 Msa0868460:intron 30.0%
CTGGAAAATAAGGAGCTAAA+GGG + chr6_1:1978037-1978056 Msa0868460:intron 35.0%
!! TGGATGTCTTGGTCTTATTA+TGG - chr6_1:1978211-1978230 Msa0868470:CDS 35.0%
GGAATTGAAGAACTAGTAGA+AGG + chr6_1:1978362-1978381 Msa0868460:intron 35.0%
GAGTATTCTTAACATCACGA+GGG + chr6_1:1978490-1978509 Msa0868460:intron 35.0%
TGAGTATTCTTAACATCACG+AGG + chr6_1:1978491-1978510 Msa0868460:intron 35.0%
TAAGGAGCTAAAGGGAAATC+TGG + chr6_1:1978029-1978048 Msa0868460:intron 40.0%
GCTGGAAAATAAGGAGCTAA+AGG + chr6_1:1978038-1978057 Msa0868460:intron 40.0%
! GGTTTATCGGCTGGAAAATA+AGG + chr6_1:1978047-1978066 Msa0868460:intron 40.0%
!!! AGCATTTTTGGTTTATCGGC+TGG + chr6_1:1978056-1978075 Msa0868460:intron 40.0%
!!! GGCGAGCATTTTTGGTTTAT+CGG + chr6_1:1978060-1978079 Msa0868460:intron 40.0%
ACCAAACAAACGATGAGCAT+TGG + chr6_1:1978081-1978100 Msa0868460:intron 40.0%
! TTTTAGCGTCTTCACCATGT+TGG + chr6_1:1978108-1978127 Msa0868460:intron 40.0%
GGAATTTGAGAACTAGTGGT+AGG + chr6_1:1978626-1978645 Msa0868460:intron 40.0%
! ACCACTAGTTCTCAAATTCC+AGG - chr6_1:1978626-1978645 Msa0868470:CDS 40.0%
ACCTGGAATTTGAGAACTAG+TGG + chr6_1:1978630-1978649 Msa0868460:intron 40.0%
!!! TGAGCATTGGCGAGCATTTT+TGG + chr6_1:1978068-1978087 Msa0868460:intron 45.0%
GCCAATGCTCATCGTTTGTT+TGG - chr6_1:1978077-1978096 Msa0868470:CDS 45.0%
TTCCCAAGTCATGGATGTCT+TGG - chr6_1:1978200-1978219 Msa0868470:CDS 45.0%
GACCAAGACATCCATGACTT+GGG + chr6_1:1978205-1978224 Msa0868460:intron 45.0%
AGACCAAGACATCCATGACT+TGG + chr6_1:1978206-1978225 Msa0868460:intron 45.0%
CGTGCAAGATTCCCAAGTCA+TGG - chr6_1:1978191-1978210 Msa0868470:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 1977963 1978700 1977963 ID=Msa0868470;Name=Msa0868470
chr6_1 mRNA 1977963 1978700 1977963 ID=Msa0868470-mRNA-1;Parent=Msa0868470;Name=Msa0868470-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|1|1|0|245
chr6_1 exon 1977963 1978700 1977963 ID=Msa0868470-mRNA-1:exon:583;Parent=Msa0868470-mRNA-1
chr6_1 CDS 1977963 1978700 1977963 ID=Msa0868470-mRNA-1:cds;Parent=Msa0868470-mRNA-1
Gene Sequence

>Msa0868470

ATGGATATTCACAGAAAAAACAAGGCATGTGCAGCCTGCAAATATCAAAGAAGAAAGTGTACTCCAGATTTCCCTTTAGCTCCTTATTTTCCAGCCGATAAACCAAAAATGCTCGCCAATGCTCATCGTTTGTTTGGTATTTCCAACATGGTGAAGACGCTAAAATCTATTGATGATGATTATCATAAAGATGAAGCAATGAAATCGATTATTTTTGAATTGGAAATGCGTGCAAGATTCCCAAGTCATGGATGTCTTGGTCTTATTATGGAGTATCAAGATATGATAATTGAGTCTATGAAAGAGTTAGATCATGTAAAACAGTTACTAAGTTTGTGTAAGCTATCACAACAACAAAATCTTCTACATTTCTCTTCATCGGATCATTCTTCAGTACCTTCTACTAGTTCTTCAATTCCAATATTTAACAATGTTGGTGATGTTCCTTATTATTATCACAATAGTGAAAATAATAATGATACTATGATGAGATCATCTTCTTCTTCTTATATTAATGACATCATCCCTCGTGATGTTAAGAATACTCAAGTTCTTGGAAATCTTGATATGATAAAGTATGAGGAAGAGTTCGATTATGTAAGAAATAATCTACAGCAGCAAAATTTTCTACCTTACTTTTCAATGGATCATTCTTCTGTACCTACCACTAGTTCTCAAATTCCAGGTATTAATCCAATATCTAGCAATGTTGGTAACATTCATTCCTCACGATGTTAA

Protein sequence

>Msa0868470

MDIHRKNKACAACKYQRRKCTPDFPLAPYFPADKPKMLANAHRLFGISNMVKTLKSIDDDYHKDEAMKSIIFELEMRARFPSHGCLGLIMEYQDMIIESMKELDHVKQLLSLCKLSQQQNLLHFSSSDHSSVPSTSSSIPIFNNVGDVPYYYHNSENNNDTMMRSSSSSYINDIIPRDVKNTQVLGNLDMIKYEEEFDYVRNNLQQQNFLPYFSMDHSSVPTTSSQIPGINPISSNVGNIHSSRC*