AlfalfaGEDB | Msa0883890

M. sativa cultivar zhongmu-4 / Msa0883890

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0883890	AES92607.2	52.846	123	51	2	45	160	167	289	7.99e-27	116
Msa0883890	AES92607.2	71.774	124	24	1	106	218	52	175	3.34e-46	167

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0883890	sp\|Q9LQQ8\|PBL5_ARATH	55.319	141	43	4	95	218	40	177	2.25e-35	132
Msa0883890	sp\|Q9LQQ8\|PBL5_ARATH	46.341	123	59	2	45	160	169	291	2.48e-24	102

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0883890	G7JI20	52.846	123	51	2	45	160	167	289	3.81e-27	116
Msa0883890	G7JI20	71.774	124	24	1	106	218	52	175	1.60e-46	167

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0883890	MtrunA17_Chr4g0074651	71.774	124	24	1	106	218	74	197	6.32e-50	167
Msa0883890	MtrunA17_Chr4g0074651	52.846	123	51	2	45	160	189	311	8.25e-31	117
Msa0883890	MtrunA17_Chr2g0327471	65.672	134	35	1	96	218	32	165	2.04e-47	160
Msa0883890	MtrunA17_Chr2g0327471	52.033	123	52	3	45	160	157	279	1.61e-30	116
Msa0883890	MtrunA17_Chr2g0327511	68.548	124	28	1	106	218	59	182	4.13e-47	159
Msa0883890	MtrunA17_Chr2g0327511	80.952	63	12	0	45	107	174	236	5.68e-29	111
Msa0883890	MtrunA17_Chr2g0327531	67.327	101	22	1	129	218	17	117	4.20e-34	124
Msa0883890	MtrunA17_Chr2g0327531	76.190	63	15	0	45	107	109	171	4.90e-27	105
Msa0883890	MtrunA17_Chr2g0327541	62.832	113	31	1	117	218	75	187	6.88e-34	125
Msa0883890	MtrunA17_Chr2g0327541	61.538	91	32	2	19	107	152	241	1.42e-26	105

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0883890	AT1G07870.1	55.319	141	43	4	95	218	40	177	2.29e-36	132
Msa0883890	AT1G07870.1	46.341	123	59	2	45	160	169	291	2.52e-25	102
Msa0883890	AT1G07870.2	55.319	141	43	4	95	218	40	177	8.08e-36	132
Msa0883890	AT1G07870.2	46.341	123	59	2	45	160	169	291	4.92e-25	102
Msa0883890	AT2G28590.2	58.559	111	33	2	119	218	64	172	1.03e-31	118
Msa0883890	AT2G28590.2	71.429	63	18	0	45	107	164	226	1.19e-24	99.4
Msa0883890	AT2G28590.1	58.559	111	33	2	119	218	64	172	2.36e-31	119
Msa0883890	AT2G28590.1	71.429	63	18	0	45	107	164	226	3.66e-24	99.4
Msa0883890	AT5G02800.1	70.833	72	20	1	36	107	131	201	1.80e-27	107
Msa0883890	AT5G02800.1	57.143	91	28	1	139	218	57	147	7.10e-27	106

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Find 44 sgRNAs with CRISPR-Local

Find 502 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACGAGAATGAGAATAGTTGA+TGG	0.479000	6_1:-36157314	Msa0883890:CDS
AGAATTTGAATTTAATAGAT+CGG	0.430270	6_1:-36149777	Msa0883890:CDS
AGAATTTGAATTTAATAGAT+CGG	0.430270	6_1:-36157703	Msa0883890:CDS
AGCTAAAGGTTTGGAGTATT+TGG	0.239532	6_1:-36157288	Msa0883890:CDS
AGGTCACGGTATATGACAAG+AGG	0.646179	6_1:+36157251	None:intergenic
AGTTTATGAGTACATGCCTT+TGG	0.355093	6_1:-36157396	Msa0883890:CDS
ATAAAGCAACTTGACCCTGA+TGG	0.558686	6_1:-36149604	Msa0883890:CDS
ATAGTTGATGGTGCAGCTAA+AGG	0.487654	6_1:-36157302	Msa0883890:CDS
ATTCAAATTCTTCACGCCTA+AGG	0.475821	6_1:+36149789	None:intergenic
ATTCAAATTCTTCACGCCTA+AGG	0.475821	6_1:+36157715	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAACACTATCAATATTCCTT+AGG	-	chr6_1:36149601-36149620	Msa0883890:CDS	30.0%
TTACATCGAGAAAATTAAGC+AGG	-	chr6_1:36149771-36149790	Msa0883890:CDS	30.0%
CCTATGAATGAATAAGTTCA+TGG	+	chr6_1:36150196-36150215	None:intergenic	30.0%
CCATGAACTTATTCATTCAT+AGG	-	chr6_1:36150193-36150212	Msa0883890:intron	30.0%
ATTTGCCCACATAATGATAT+TGG	+	chr6_1:36150563-36150582	None:intergenic	30.0%
GAAAGAGTCTTAATCAACTA+AGG	+	chr6_1:36150626-36150645	None:intergenic	30.0%
ACAATAACGATAAACATCCA+TGG	+	chr6_1:36150951-36150970	None:intergenic	30.0%
TTTCCCATTATTCCGTTAAT+GGG	+	chr6_1:36151769-36151788	None:intergenic	30.0%
TACTGTCCTTATTGTCTAAT+TGG	+	chr6_1:36152456-36152475	None:intergenic	30.0%
GTAACAGGTTACATATCAAT+TGG	-	chr6_1:36152557-36152576	Msa0883890:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	36149486	36157868	36149486	ID=Msa0883890;Name=Msa0883890
chr6_1	mRNA	36149486	36157868	36149486	ID=Msa0883890-mRNA-1;Parent=Msa0883890;Name=Msa0883890-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0\|0\|0\|1\|0\|0\|5\|0\|218
chr6_1	exon	36157863	36157868	36157863	ID=Msa0883890-mRNA-1:exon:8607;Parent=Msa0883890-mRNA-1
chr6_1	exon	36157691	36157819	36157691	ID=Msa0883890-mRNA-1:exon:8606;Parent=Msa0883890-mRNA-1
chr6_1	exon	36157248	36157435	36157248	ID=Msa0883890-mRNA-1:exon:8605;Parent=Msa0883890-mRNA-1
chr6_1	exon	36149669	36149849	36149669	ID=Msa0883890-mRNA-1:exon:8604;Parent=Msa0883890-mRNA-1
chr6_1	exon	36149486	36149635	36149486	ID=Msa0883890-mRNA-1:exon:8603;Parent=Msa0883890-mRNA-1
chr6_1	CDS	36157863	36157868	36157863	ID=Msa0883890-mRNA-1:cds;Parent=Msa0883890-mRNA-1
chr6_1	CDS	36157691	36157819	36157691	ID=Msa0883890-mRNA-1:cds;Parent=Msa0883890-mRNA-1
chr6_1	CDS	36157248	36157435	36157248	ID=Msa0883890-mRNA-1:cds;Parent=Msa0883890-mRNA-1
chr6_1	CDS	36149669	36149849	36149669	ID=Msa0883890-mRNA-1:cds;Parent=Msa0883890-mRNA-1
chr6_1	CDS	36149486	36149635	36149486	ID=Msa0883890-mRNA-1:cds;Parent=Msa0883890-mRNA-1

Gene Sequence

>Msa0883890

ATGAAAAAGACCGAAAATAAGGTTAAAGTTGATTTGAATTTGAATGTGAGTGAGAAAAGAGAAGATGGTTCTGAACACTATCAATATTCCTTAGGCGTGAAGAATTTGAATTTAATAGATCGGGTTTCCAGGGATGGAGAGCAGACACATTTAGTTTATGAGTACATGCCTTTGGGATCTTTGGAGAATCATTTGCATCATCTTTCTCCCGGGGAAAAACCACTAGATTGGAATACGAGAATGAGAATAGTTGATGGTGCAGCTAAAGGTTTGGAGTATTTGGATGATAAAATGAAGCCTCTTGTCATATACCGTGACCTCAATGAGAAAAGAGAAGATGGTTCTGAACACTATCAATATTCCTTAGGCGTGAAGAATTTGAATTTAATAGATCGGGTTTCCACGGATGTAAAAGTTGCAAAGACATTTACTTTTGATGAGCTTGCAGCCGCAACAGAGAATTTTAAGGCAGATTGCTTTGTTGGTGAAGGAAGGTTTGGCAAGGTTGTTGCAATAAAGCAACTTGACCCTGATGGACTTCAAGGAACCAAAGAATTTGTTGTTGAAGTGTTAACACTTGGTTTGGCAGAACGCCCAAATCTTGTCAATTTATTAGGATTTTGTGCTGAGGGAGAGCAGAGACATTTGGATTAT

Protein sequence

>Msa0883890

MKKTENKVKVDLNLNVSEKREDGSEHYQYSLGVKNLNLIDRVSRDGEQTHLVYEYMPLGSLENHLHHLSPGEKPLDWNTRMRIVDGAAKGLEYLDDKMKPLVIYRDLNEKREDGSEHYQYSLGVKNLNLIDRVSTDVKVAKTFTFDELAAATENFKADCFVGEGRFGKVVAIKQLDPDGLQGTKEFVVEVLTLGLAERPNLVNLLGFCAEGEQRHLDY