AlfalfaGEDB | Msa0886210

M. sativa cultivar zhongmu-4 / Msa0886210

KEGG: -
GO: GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886210	PNX75215.1	90.714	140	13	0	89	228	1	140	6.11e-85	266

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886210	sp\|Q9P6P3\|PPK15_SCHPO	36.000	150	86	2	59	207	88	228	1.56e-19	90.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886210	A0A2K3L9K7	90.714	140	13	0	89	228	1	140	2.92e-85	266

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886210	MtrunA17_Chr5g0437131	88.079	151	18	0	78	228	371	521	1.02e-87	273
Msa0886210	MtrunA17_Chr6g0480541	75.000	140	35	0	89	228	877	1016	3.15e-68	226
Msa0886210	MtrunA17_Chr6g0480481	73.404	94	25	0	90	183	14	107	8.87e-46	151
Msa0886210	MtrunA17_Chr5g0421231	72.277	101	20	1	3	95	403	503	8.33e-38	139
Msa0886210	MtrunA17_Chr8g0369241	37.931	145	79	4	91	228	114	254	4.00e-21	91.7
Msa0886210	MtrunA17_Chr3g0077671	75.000	68	9	1	30	89	1	68	4.23e-21	89.4
Msa0886210	MtrunA17_Chr4g0070771	83.333	36	6	0	3	38	952	987	2.69e-12	65.9
Msa0886210	MtrunA17_Chr1g0210061	36.538	104	61	2	109	207	609	712	7.86e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0886210	AT2G40120.1	81.429	140	26	0	89	228	240	379	7.56e-78	244
Msa0886210	AT1G73450.1	73.571	140	37	0	89	228	820	959	1.10e-66	223
Msa0886210	AT1G73460.2	73.571	140	37	0	89	228	837	976	1.14e-66	223
Msa0886210	AT1G73460.1	73.571	140	37	0	89	228	837	976	1.14e-66	223
Msa0886210	AT3G17750.1	74.286	140	36	0	89	228	806	945	1.21e-66	222
Msa0886210	AT5G35980.3	39.344	122	69	2	108	228	85	202	3.00e-19	86.7
Msa0886210	AT5G35980.1	39.344	122	69	2	108	228	120	237	3.02e-19	86.7
Msa0886210	AT5G35980.2	39.344	122	69	2	108	228	120	237	4.89e-19	85.9

Find 47 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAAAGATTAACTCTTTAA+TGG	0.182382	6_1:+44521179	Msa0886210:CDS
ACAGGAGTCTCTTTAATAAT+TGG	0.190067	6_1:-44521141	None:intergenic
ATGCTTCACCACCAGATTCT+TGG	0.279095	6_1:-44521719	None:intergenic
CAGAAATTCAACCAAGAATC+TGG	0.301956	6_1:+44521708	Msa0886210:CDS
TCAGTATCTTCAATTATAAC+AGG	0.330980	6_1:-44521159	None:intergenic
TGATTCCCTTCTGCATATCA+TGG	0.336809	6_1:-44521491	None:intergenic
CTCATGGAATTGCATGTAGA+TGG	0.363694	6_1:+44521342	Msa0886210:CDS
GTTCATTACTCGACAGTGTT+TGG	0.390722	6_1:+44521764	Msa0886210:CDS
GTCGAGCCAGAACGCTGCTT+TGG	0.395848	6_1:-44521233	None:intergenic
TAAGAAGCTTGATTTCATCT+AGG	0.411237	6_1:-44521563	None:intergenic
ATGTAGATGGGGTTCAGTTC+AGG	0.412337	6_1:+44521355	Msa0886210:CDS
GCCAGAACGCTGCTTTGGTC+TGG	0.422121	6_1:-44521228	None:intergenic
GTTATCTAACATGGATGAAA+AGG	0.423280	6_1:+44521101	None:intergenic
AATTGGAGGTGCTTCTTGAA+TGG	0.430261	6_1:-44521124	None:intergenic
TTGACCGTGTTCAGCACAAT+AGG	0.437368	6_1:-44521405	None:intergenic
TCGGTTCAGCTGCCTTCAGT+AGG	0.441041	6_1:+44521457	Msa0886210:CDS
TTATGACTATTTCTACCATC+AGG	0.460782	6_1:+44521635	Msa0886210:CDS
GATTCCCTTCTGCATATCAT+GGG	0.467704	6_1:-44521490	None:intergenic
AGTATGTGGTGTTTATCTCC+CGG	0.479535	6_1:-44521606	None:intergenic
TAAGTTTGCACGCTGAAGTT+CGG	0.485105	6_1:-44521676	None:intergenic
GGAGTCTCTTTAATAATTGG+AGG	0.493895	6_1:-44521138	None:intergenic
CAGCCTTCACATTGGACTCA+TGG	0.496835	6_1:+44521326	Msa0886210:CDS
TCATGGAATTGCATGTAGAT+GGG	0.505051	6_1:+44521343	Msa0886210:CDS
AGAACGCTGCTTTGGTCTGG+TGG	0.506341	6_1:-44521225	None:intergenic
GATGGGGTTCAGTTCAGGAC+TGG	0.518408	6_1:+44521360	Msa0886210:CDS
ACCAGACCAAAGCAGCGTTC+TGG	0.522121	6_1:+44521227	Msa0886210:CDS
CGGTTCAGCTGCCTTCAGTA+GGG	0.526607	6_1:+44521458	Msa0886210:CDS
GTATGTGGTGTTTATCTCCC+GGG	0.540528	6_1:-44521605	None:intergenic
CATTACTCGACAGTGTTTGG+AGG	0.548506	6_1:+44521767	Msa0886210:CDS
AGTCATAAAGACGCAGTATG+TGG	0.571598	6_1:-44521620	None:intergenic
CCGCATCACAGCCTTCACAT+TGG	0.588508	6_1:+44521318	Msa0886210:CDS
TATTGTGCTGAACACGGTCA+AGG	0.598687	6_1:+44521407	Msa0886210:CDS
CAATGAATAAATGTTCCTGA+TGG	0.601057	6_1:-44521650	None:intergenic
TACTATGTGACAGAATCTCT+CGG	0.603979	6_1:+44521438	Msa0886210:CDS
GACTGGATTTGAAGAAAACA+AGG	0.607459	6_1:+44521377	Msa0886210:CDS
TCAAGCCCATGATATGCAGA+AGG	0.611349	6_1:+44521485	Msa0886210:CDS
CATGGAATTGCATGTAGATG+GGG	0.623597	6_1:+44521344	Msa0886210:CDS
TGAACACGGTCAAGGCTGAA+AGG	0.623883	6_1:+44521415	Msa0886210:CDS
GGCTTGAACAACCCTACTGA+AGG	0.627495	6_1:-44521469	None:intergenic
CAAGCCCATGATATGCAGAA+GGG	0.638397	6_1:+44521486	Msa0886210:CDS
ACTTGTCAATAAGCACGACC+CGG	0.643370	6_1:+44521587	Msa0886210:CDS
CTTGTCAATAAGCACGACCC+GGG	0.659914	6_1:+44521588	Msa0886210:CDS
ATGTGAAGGCTGTGATGCGG+CGG	0.662563	6_1:-44521315	None:intergenic
AGTGCCTATTGTGCTGAACA+CGG	0.662852	6_1:+44521401	Msa0886210:CDS
AAATTCAACCAAGAATCTGG+TGG	0.683493	6_1:+44521711	Msa0886210:CDS
ATTCCATGAGTCCAATGTGA+AGG	0.687752	6_1:-44521329	None:intergenic
CCAATGTGAAGGCTGTGATG+CGG	0.688876	6_1:-44521318	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAAAAGATTAACTCTTTAA+TGG	+	chr6_1:44521179-44521198	Msa0886210:CDS	20.0%
!!	GAAGATTATTAAAAATGACA+AGG	+	chr6_1:44521524-44521543	Msa0886210:CDS	20.0%
!	TCAGTATCTTCAATTATAAC+AGG	-	chr6_1:44521162-44521181	None:intergenic	25.0%
	ACAGGAGTCTCTTTAATAAT+TGG	-	chr6_1:44521144-44521163	None:intergenic	30.0%
	TAAGAAGCTTGATTTCATCT+AGG	-	chr6_1:44521566-44521585	None:intergenic	30.0%
!!	TTATGACTATTTCTACCATC+AGG	+	chr6_1:44521635-44521654	Msa0886210:CDS	30.0%
	CAATGAATAAATGTTCCTGA+TGG	-	chr6_1:44521653-44521672	None:intergenic	30.0%
	GGAGTCTCTTTAATAATTGG+AGG	-	chr6_1:44521141-44521160	None:intergenic	35.0%
	TCATGGAATTGCATGTAGAT+GGG	+	chr6_1:44521343-44521362	Msa0886210:CDS	35.0%
	GACTGGATTTGAAGAAAACA+AGG	+	chr6_1:44521377-44521396	Msa0886210:CDS	35.0%
	TACTATGTGACAGAATCTCT+CGG	+	chr6_1:44521438-44521457	Msa0886210:CDS	35.0%
	CAGAAATTCAACCAAGAATC+TGG	+	chr6_1:44521708-44521727	Msa0886210:CDS	35.0%
	AAATTCAACCAAGAATCTGG+TGG	+	chr6_1:44521711-44521730	Msa0886210:CDS	35.0%
!!	AATTGGAGGTGCTTCTTGAA+TGG	-	chr6_1:44521127-44521146	None:intergenic	40.0%
	ATTCCATGAGTCCAATGTGA+AGG	-	chr6_1:44521332-44521351	None:intergenic	40.0%
	CTCATGGAATTGCATGTAGA+TGG	+	chr6_1:44521342-44521361	Msa0886210:CDS	40.0%
	CATGGAATTGCATGTAGATG+GGG	+	chr6_1:44521344-44521363	Msa0886210:CDS	40.0%
	GATTCCCTTCTGCATATCAT+GGG	-	chr6_1:44521493-44521512	None:intergenic	40.0%
	TGATTCCCTTCTGCATATCA+TGG	-	chr6_1:44521494-44521513	None:intergenic	40.0%
	AGTATGTGGTGTTTATCTCC+CGG	-	chr6_1:44521609-44521628	None:intergenic	40.0%
	AGTCATAAAGACGCAGTATG+TGG	-	chr6_1:44521623-44521642	None:intergenic	40.0%
	TAAGTTTGCACGCTGAAGTT+CGG	-	chr6_1:44521679-44521698	None:intergenic	40.0%
	GTTCATTACTCGACAGTGTT+TGG	+	chr6_1:44521764-44521783	Msa0886210:CDS	40.0%
	ATGTAGATGGGGTTCAGTTC+AGG	+	chr6_1:44521355-44521374	Msa0886210:CDS	45.0%
	AGTGCCTATTGTGCTGAACA+CGG	+	chr6_1:44521401-44521420	Msa0886210:CDS	45.0%
	TTGACCGTGTTCAGCACAAT+AGG	-	chr6_1:44521408-44521427	None:intergenic	45.0%
	TATTGTGCTGAACACGGTCA+AGG	+	chr6_1:44521407-44521426	Msa0886210:CDS	45.0%
	TCAAGCCCATGATATGCAGA+AGG	+	chr6_1:44521485-44521504	Msa0886210:CDS	45.0%
	CAAGCCCATGATATGCAGAA+GGG	+	chr6_1:44521486-44521505	Msa0886210:CDS	45.0%
	ACTTGTCAATAAGCACGACC+CGG	+	chr6_1:44521587-44521606	Msa0886210:CDS	45.0%
	GTATGTGGTGTTTATCTCCC+GGG	-	chr6_1:44521608-44521627	None:intergenic	45.0%
	ATGCTTCACCACCAGATTCT+TGG	-	chr6_1:44521722-44521741	None:intergenic	45.0%
	CATTACTCGACAGTGTTTGG+AGG	+	chr6_1:44521767-44521786	Msa0886210:CDS	45.0%
	CCAATGTGAAGGCTGTGATG+CGG	-	chr6_1:44521321-44521340	None:intergenic	50.0%
	CAGCCTTCACATTGGACTCA+TGG	+	chr6_1:44521326-44521345	Msa0886210:CDS	50.0%
	TGAACACGGTCAAGGCTGAA+AGG	+	chr6_1:44521415-44521434	Msa0886210:CDS	50.0%
	GGCTTGAACAACCCTACTGA+AGG	-	chr6_1:44521472-44521491	None:intergenic	50.0%
	CTTGTCAATAAGCACGACCC+GGG	+	chr6_1:44521588-44521607	Msa0886210:CDS	50.0%
!	AGAACGCTGCTTTGGTCTGG+TGG	-	chr6_1:44521228-44521247	None:intergenic	55.0%
	ACCAGACCAAAGCAGCGTTC+TGG	+	chr6_1:44521227-44521246	Msa0886210:CDS	55.0%
	ATGTGAAGGCTGTGATGCGG+CGG	-	chr6_1:44521318-44521337	None:intergenic	55.0%
	CCGCATCACAGCCTTCACAT+TGG	+	chr6_1:44521318-44521337	Msa0886210:CDS	55.0%
	GATGGGGTTCAGTTCAGGAC+TGG	+	chr6_1:44521360-44521379	Msa0886210:CDS	55.0%
	TCGGTTCAGCTGCCTTCAGT+AGG	+	chr6_1:44521457-44521476	Msa0886210:CDS	55.0%
	CGGTTCAGCTGCCTTCAGTA+GGG	+	chr6_1:44521458-44521477	Msa0886210:CDS	55.0%
!	GCCAGAACGCTGCTTTGGTC+TGG	-	chr6_1:44521231-44521250	None:intergenic	60.0%
	GTCGAGCCAGAACGCTGCTT+TGG	-	chr6_1:44521236-44521255	None:intergenic	60.0%
!	TGATGCGGCGGTTTTGCTGG+TGG	-	chr6_1:44521306-44521325	None:intergenic	60.0%
!	CTGTGATGCGGCGGTTTTGC+TGG	-	chr6_1:44521309-44521328	None:intergenic	60.0%
!	GCGGCGGTTTTGCTGGTGGT+CGG	-	chr6_1:44521302-44521321	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	44521111	44521797	44521111	ID=Msa0886210;Name=Msa0886210
chr6_1	mRNA	44521111	44521797	44521111	ID=Msa0886210-mRNA-1;Parent=Msa0886210;Name=Msa0886210-mRNA-1;_AED=0.62;_eAED=0.62;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|228
chr6_1	exon	44521111	44521797	44521111	ID=Msa0886210-mRNA-1:exon:9551;Parent=Msa0886210-mRNA-1
chr6_1	CDS	44521111	44521797	44521111	ID=Msa0886210-mRNA-1:cds;Parent=Msa0886210-mRNA-1

Gene Sequence

>Msa0886210

ATGGATGAAAAGGCCATTCAAGAAGCACCTCCAATTATTAAAGAGACTCCTGTTATAATTGAAGATACTGAAAAGATTAACTCTTTAATGGCTGAAGTCAATAGTTTGAAGAAACCACCAGACCAAAGCAGCGTTCTGGCTCGACTCAAACTGCAGAACTTGAGTACTTATGTTGAGATGAATGAGCTCCGACCACCAGCAAAACCGCCGCATCACAGCCTTCACATTGGACTCATGGAATTGCATGTAGATGGGGTTCAGTTCAGGACTGGATTTGAAGAAAACAAGGAAGTGCCTATTGTGCTGAACACGGTCAAGGCTGAAAGGTACTATGTGACAGAATCTCTCGGTTCAGCTGCCTTCAGTAGGGTTGTTCAAGCCCATGATATGCAGAAGGGAATCAAAGTTTGTTTGAAGATTATTAAAAATGACAAGGATTTCTTTGATCAAAGCCTAGATGAAATCAAGCTTCTTAAACTTGTCAATAAGCACGACCCGGGAGATAAACACCACATACTGCGTCTTTATGACTATTTCTACCATCAGGAACATTTATTCATTGTGACCGAACTTCAGCGTGCAAACTTATATGAATTTCAGAAATTCAACCAAGAATCTGGTGGTGAAGCATATTTTACATTGAAGAGATTGCAGTTCATTACTCGACAGTGTTTGGAGGCATTGTAA

Protein sequence

>Msa0886210

MDEKAIQEAPPIIKETPVIIEDTEKINSLMAEVNSLKKPPDQSSVLARLKLQNLSTYVEMNELRPPAKPPHHSLHIGLMELHVDGVQFRTGFEENKEVPIVLNTVKAERYYVTESLGSAAFSRVVQAHDMQKGIKVCLKIIKNDKDFFDQSLDEIKLLKLVNKHDPGDKHHILRLYDYFYHQEHLFIVTELQRANLYEFQKFNQESGGEAYFTLKRLQFITRQCLEAL*