AlfalfaGEDB | Msa0889430

M. sativa cultivar zhongmu-4 / Msa0889430

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0889430	KEH25323.1	88.764	89	10	0	17	105	41	129	3.64e-50	167

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0889430	A0A072U878	88.764	89	10	0	17	105	41	129	1.74e-50	167

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TFs/TRs:

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Gene ID	Type	Classification
Msa0889430	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0889430	MtrunA17_Chr3g0082121	65.476	84	29	22	105	31	114	1.63e-31	117
Msa0889430	MtrunA17_Chr5g0401121	59.524	84	34	22	105	44	127	5.73e-28	101
Msa0889430	MtrunA17_Chr6g0458391	53.030	66	31	28	93	219	284	1.69e-17	77.8
Msa0889430	MtrunA17_Chr6g0458391	44.615	65	36	33	97	90	154	3.96e-11	59.3
Msa0889430	MtrunA17_Chr1g0203721	38.272	81	50	25	105	54	134	2.22e-12	63.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTCACATGCTTCAAAG+AGG	0.633613	6_1:-57105194	Msa0889430:CDS
AATGAAGTTTGATTCTGAGG+CGG	0.710835	6_1:-57105317	Msa0889430:CDS
ACAAAAGACGTCAACCAAAA+GGG	0.496414	6_1:-57105156	Msa0889430:CDS
AGATTCACATGCTTCAAAGA+GGG	0.643478	6_1:-57105193	Msa0889430:CDS
ATAAAGCAGAAATTAGAAAT+TGG	0.362913	6_1:-57105129	Msa0889430:CDS
ATGTATGTGTCAACTTGATT+TGG	0.422918	6_1:+57105024	None:intergenic
ATTGAGTACAGTAAAAGAAT+TGG	0.326573	6_1:-57105277	Msa0889430:CDS
CAAAAGACGTCAACCAAAAG+GGG	0.607006	6_1:-57105155	Msa0889430:CDS
CAATCAAGTCAAGTTGGAAA+TGG	0.427480	6_1:-57105081	Msa0889430:CDS
CAGAAATTAGAAATTGGAAA+TGG	0.429148	6_1:-57105123	Msa0889430:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAACTTCATTTCCAAGTA+AGG	+	chr6_1:57105222-57105241	None:intergenic	30.0%
ATGCCTGCAATTATCAGTTA+TGG	-	chr6_1:57105088-57105107	Msa0889430:CDS	35.0%
TTCCAAGTAAGGTACATTGT+TGG	+	chr6_1:57105211-57105230	None:intergenic	35.0%
GGAAATGAAGTTTGATTCTG+AGG	-	chr6_1:57105227-57105246	Msa0889430:CDS	35.0%
TTTGGTATTCGTCGAGAATA+TGG	-	chr6_1:57105294-57105313	Msa0889430:CDS	35.0%
GGAAATAAAAGCAGAGTAGA+TGG	-	chr6_1:57105315-57105334	Msa0889430:CDS	35.0%
AAGATTCACATGCTTCAAAG+AGG	-	chr6_1:57105353-57105372	Msa0889430:CDS	35.0%
AGATTCACATGCTTCAAAGA+GGG	-	chr6_1:57105354-57105373	Msa0889430:CDS	35.0%
ACAAAAGACGTCAACCAAAA+GGG	-	chr6_1:57105391-57105410	Msa0889430:intron	35.0%
CCACCATAACTGATAATTGC+AGG	+	chr6_1:57105094-57105113	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_1	gene	57105033	57105536	57105033	ID=Msa0889430;Name=Msa0889430
chr6_1	mRNA	57105033	57105536	57105033	ID=Msa0889430-mRNA-1;Parent=Msa0889430;Name=Msa0889430-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|138
chr6_1	exon	57105490	57105536	57105490	ID=Msa0889430-mRNA-1:exon:11172;Parent=Msa0889430-mRNA-1
chr6_1	exon	57105033	57105402	57105033	ID=Msa0889430-mRNA-1:exon:11171;Parent=Msa0889430-mRNA-1
chr6_1	CDS	57105490	57105536	57105490	ID=Msa0889430-mRNA-1:cds;Parent=Msa0889430-mRNA-1
chr6_1	CDS	57105033	57105402	57105033	ID=Msa0889430-mRNA-1:cds;Parent=Msa0889430-mRNA-1

Gene Sequence

>Msa0889430

ATGCAGTCTAAGTTCTTCAAGTATATTGATTTGAGACATTGTTTCATCATTCATGAACAGAATGTGCAACGCGGACAAGGAAGTATTGCCAACAATGTACCTTACTTGGAAATGAAGTTTGATTCTGAGGCGGTGGCTTATGGATTTTATATTGAGTACAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAGTAGATGGAGTTTTAACTTCAAGAAGATTCACATGCTTCAAAGAGGGTGTACGAGGTGTTGACAAAAGACGTCAACCAAAAGGGGAGCATAAAGCAGAAATTAGAAATTGGAAATGGAACTACAGTTCAAGCGACACAATCAAGTCAAGTTGGAAATGGAACTCAACATCAAGCTACAATCCAAGAGTCATAACCAAATCAAGTTGA

Protein sequence

>Msa0889430

MQSKFFKYIDLRHCFIIHEQNVQRGQGSIANNVPYLEMKFDSEAVAYGFYIEYSKRIGFGIRREYGNKSRVDGVLTSRRFTCFKEGVRGVDKRRQPKGEHKAEIRNWKWNYSSSDTIKSSWKWNSTSSYNPRVITKSS*