AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0891930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0891930 MtrunA17_Chr3g0082121 71.037 694 189 3 1 690 8 693 0.0 1016
Msa0891930 MtrunA17_Chr2g0324011 44.887 577 309 6 34 604 10 583 1.89e-170 508
Msa0891930 MtrunA17_Chr1g0164591 72.381 315 79 1 112 426 1 307 4.10e-169 494
Msa0891930 MtrunA17_Chr6g0458391 40.246 569 315 6 27 590 218 766 1.45e-147 454
Msa0891930 MtrunA17_Chr6g0458391 37.594 133 81 2 33 165 90 220 7.27e-21 98.6
Msa0891930 MtrunA17_Chr1g0203721 37.750 649 383 8 36 670 65 706 6.80e-144 449
Msa0891930 MtrunA17_Chr5g0448761 34.098 654 415 7 34 675 60 709 3.89e-123 391
Msa0891930 MtrunA17_Chr1g0202341 33.498 606 389 7 34 630 51 651 1.80e-105 342
Msa0891930 MtrunA17_Chr8g0363841 31.818 572 361 10 35 589 18 577 2.38e-96 317
Msa0891930 MtrunA17_Chr2g0287651 30.804 659 417 13 36 680 77 710 1.65e-92 311
Msa0891930 MtrunA17_Chr1g0207551 29.032 713 467 12 34 738 34 715 3.89e-89 301
Msa0891930 MtrunA17_Chr2g0329031 29.853 680 417 19 34 686 14 660 2.33e-85 289
Msa0891930 MtrunA17_Chr3g0080351 29.228 674 417 15 31 680 73 710 9.44e-83 284
Msa0891930 MtrunA17_Chr5g0441581 29.094 684 428 20 1 658 1 653 1.86e-79 272
Msa0891930 MtrunA17_Chr7g0258861 48.239 284 139 6 34 312 10 290 5.06e-79 258
Msa0891930 MtrunA17_Chr5g0440451 31.013 632 382 16 32 634 41 647 4.37e-78 273
Msa0891930 MtrunA17_Chr2g0296781 31.065 573 357 14 38 589 1 556 1.06e-77 268
Msa0891930 MtrunA17_Chr2g0276841 30.195 563 377 10 34 586 70 626 4.54e-76 263
Msa0891930 MtrunA17_Chr3g0118611 34.305 446 280 7 213 651 122 561 3.41e-75 262
Msa0891930 MtrunA17_Chr1g0198761 30.594 572 371 13 37 590 50 613 2.06e-73 256
Msa0891930 MtrunA17_Chr5g0448511 30.785 484 322 5 197 675 1 476 1.69e-71 246
Msa0891930 MtrunA17_Chr1g0203051 27.778 558 363 9 39 576 84 621 3.27e-71 248
Msa0891930 MtrunA17_Chr2g0294841 26.935 672 445 14 26 676 37 683 8.47e-71 249
Msa0891930 MtrunA17_Chr2g0290391 27.055 669 431 17 34 672 51 692 2.03e-65 233
Msa0891930 MtrunA17_Chr6g0477261 31.264 451 282 10 158 589 4 445 1.34e-64 229
Msa0891930 MtrunA17_Chr1g0149131 83.929 112 18 0 313 424 1 112 9.80e-64 210
Msa0891930 MtrunA17_Chr2g0287061 26.871 588 351 14 25 591 174 703 2.81e-61 223
Msa0891930 MtrunA17_Chr1g0196541 27.513 567 379 14 38 586 1 553 3.15e-58 213
Msa0891930 MtrunA17_Chr2g0291581 26.970 571 384 10 33 589 70 621 9.42e-57 208
Msa0891930 MtrunA17_Chr5g0431001 29.890 455 295 11 11 446 34 483 3.37e-52 191
Msa0891930 MtrunA17_Chr2g0291591 27.368 475 336 6 34 507 64 530 8.41e-52 191
Msa0891930 MtrunA17_Chr5g0401121 62.857 140 48 1 1 140 27 162 5.85e-49 171
Msa0891930 MtrunA17_Chr4g0033611 41.667 108 57 3 34 139 76 179 5.50e-17 82.0
Msa0891930 MtrunA17_Chr2g0329421 39.815 108 59 3 34 139 27 130 7.61e-17 80.5
Msa0891930 MtrunA17_Chr7g0238821 39.450 109 62 3 38 145 1 106 2.16e-15 73.2
Msa0891930 MtrunA17_Chr3g0078041 38.095 105 63 2 34 137 50 153 1.33e-13 71.2
Msa0891930 MtrunA17_Chr2g0308841 22.727 220 159 6 380 591 13 229 7.16e-13 69.7
Msa0891930 MtrunA17_Chr2g0329361 34.752 141 81 3 34 172 61 192 7.63e-13 69.7
Msa0891930 MtrunA17_Chr7g0233911 33.945 109 62 3 34 137 34 137 7.91e-13 68.6
Msa0891930 MtrunA17_Chr3g0088371 29.670 182 116 5 553 725 2 180 2.14e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0891930 AT4G38180.1 34.234 666 423 9 1 655 39 700 8.53e-120 383
Msa0891930 AT4G15090.1 29.130 690 471 8 34 717 51 728 3.21e-93 313
Msa0891930 AT4G15090.5 29.130 690 471 8 34 717 51 728 3.21e-93 313
Msa0891930 AT4G15090.3 29.130 690 471 8 34 717 51 728 3.21e-93 313
Msa0891930 AT4G15090.2 29.130 690 471 8 34 717 51 728 3.21e-93 313
Msa0891930 AT4G15090.4 29.130 690 471 8 34 717 51 728 3.21e-93 313
Msa0891930 AT3G22170.2 29.777 628 380 12 31 634 67 657 2.26e-86 295
Msa0891930 AT3G22170.1 29.777 628 380 12 31 634 67 657 2.26e-86 295
Msa0891930 AT1G76320.3 30.909 605 389 13 38 634 1 584 3.72e-86 290
Msa0891930 AT1G76320.2 30.909 605 389 13 38 634 1 584 1.50e-85 290
Msa0891930 AT1G76320.1 30.909 605 389 13 38 634 1 584 1.58e-85 290
Msa0891930 AT1G76320.4 30.909 605 389 13 38 634 1 584 1.58e-85 290
Msa0891930 AT2G27110.2 30.658 623 376 16 26 634 41 621 2.59e-83 286
Msa0891930 AT2G27110.1 30.658 623 376 16 26 634 41 621 2.59e-83 286
Msa0891930 AT1G10240.2 28.356 663 431 15 2 644 25 663 3.95e-73 254
Msa0891930 AT1G10240.1 28.356 663 431 15 2 644 25 663 3.95e-73 254
Msa0891930 AT3G06250.2 28.496 565 349 12 34 591 190 706 2.78e-72 254
Msa0891930 AT3G06250.1 28.496 565 349 12 34 591 190 706 2.78e-72 254
Msa0891930 AT3G06250.3 28.496 565 349 12 34 591 190 706 2.78e-72 254
Msa0891930 AT2G27110.3 34.023 435 261 9 213 634 55 476 5.10e-71 249
Msa0891930 AT5G18960.2 28.055 581 359 16 23 591 197 730 1.62e-68 244
Msa0891930 AT5G18960.2 31.933 119 67 2 18 136 27 131 4.97e-12 70.5
Msa0891930 AT5G18960.1 28.055 581 359 16 23 591 197 730 1.62e-68 244
Msa0891930 AT5G18960.1 31.933 119 67 2 18 136 27 131 4.97e-12 70.5
Msa0891930 AT2G32250.5 24.613 646 431 11 37 676 41 636 2.12e-63 229
Msa0891930 AT2G32250.6 24.613 646 431 11 37 676 41 636 4.11e-63 229
Msa0891930 AT2G32250.1 24.613 646 431 11 37 676 41 636 4.11e-63 229
Msa0891930 AT2G32250.3 24.613 646 431 11 37 676 41 636 4.11e-63 229
Msa0891930 AT2G32250.2 24.613 646 431 11 37 676 41 636 5.25e-63 228
Msa0891930 AT2G32250.4 24.613 646 431 11 37 676 41 636 5.25e-63 228
Msa0891930 AT2G32250.8 24.496 645 431 11 38 676 1 595 1.96e-62 226
Msa0891930 AT2G32250.7 24.496 645 431 11 38 676 1 595 2.16e-62 226
Msa0891930 AT5G28530.1 25.415 602 408 14 34 609 55 641 4.77e-58 212
Msa0891930 AT5G28530.3 25.249 602 405 14 34 609 55 637 8.22e-58 211
Msa0891930 AT5G28530.2 25.249 602 405 14 34 609 55 637 8.22e-58 211
Msa0891930 AT4G38170.1 29.091 385 261 6 213 590 21 400 7.84e-57 205
Msa0891930 AT4G19990.3 27.516 636 382 13 37 643 24 609 1.88e-56 208
Msa0891930 AT4G19990.4 27.516 636 382 13 37 643 24 609 1.88e-56 208
Msa0891930 AT4G19990.5 27.516 636 382 13 37 643 24 609 2.03e-56 208
Msa0891930 AT4G19990.7 27.516 636 382 13 37 643 38 623 3.03e-56 208
Msa0891930 AT4G19990.6 27.373 632 388 12 37 643 24 609 3.18e-56 208
Msa0891930 AT4G19990.2 27.373 632 388 12 37 643 24 609 3.18e-56 208
Msa0891930 AT1G80010.1 26.368 603 412 13 19 599 80 672 7.98e-53 197
Msa0891930 AT1G52520.1 26.976 582 373 16 33 589 84 638 4.95e-52 195
Msa0891930 AT4G19990.1 26.756 598 367 12 71 643 1 552 7.16e-48 182
Msa0891930 AT5G28530.4 23.639 588 373 16 34 609 55 578 2.59e-40 158
Msa0891930 AT5G28530.5 23.630 584 374 15 34 609 55 574 5.65e-40 157
Msa0891930 AT3G59470.3 40.566 106 61 2 34 139 70 173 2.60e-17 83.2
Msa0891930 AT3G59470.1 40.566 106 61 2 34 139 70 173 2.60e-17 83.2
Msa0891930 AT3G59470.2 40.566 106 61 2 34 139 82 185 4.05e-17 82.8
Msa0891930 AT3G07500.1 34.247 146 86 3 34 179 33 168 5.77e-15 75.5
Msa0891930 AT4G12850.4 34.921 126 75 3 34 159 11 129 3.71e-14 70.9
Msa0891930 AT2G43280.1 38.261 115 67 3 34 145 22 135 5.24e-14 72.4
Msa0891930 AT4G12850.2 37.864 103 60 2 34 136 11 109 9.66e-13 66.6
Msa0891930 AT4G12850.1 37.864 103 60 2 34 136 11 109 1.87e-12 67.4

Find 159 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTAATAACAAATATATTT+TGG 0.155788 6_1:-68744510 Msa0891930:CDS
TCTTTCTAAGCTTCTGTTAT+TGG 0.203806 6_1:-68742376 Msa0891930:CDS
TTCCAGATTGTGTCGATAAA+TGG 0.224458 6_1:-68745200 Msa0891930:CDS
TTCATCATTTGCAATTGATA+TGG 0.235636 6_1:+68743214 None:intergenic
TTTGAAGTCAGATATAAATT+TGG 0.265489 6_1:-68743891 Msa0891930:CDS
GCGGATCCTTGTGTGGATTC+TGG 0.277921 6_1:-68742450 Msa0891930:CDS
GCGCACAATTGGAGTGTTTA+GGG 0.285651 6_1:-68742310 Msa0891930:CDS
TTTGGTATTCGTCGAGAATA+TGG 0.287007 6_1:-68745042 Msa0891930:CDS
CTTTAGTGATTGCTTCCTTT+TGG 0.296517 6_1:+68744621 None:intergenic
GCACAGATGATTAATGATTA+TGG 0.297180 6_1:-68744481 Msa0891930:CDS
AATGATTACAGTAAAAGAAT+TGG 0.319753 6_1:-68745066 Msa0891930:CDS
ATGAGATTCTGAAGCTCTAT+TGG 0.322184 6_1:+68743346 None:intergenic
TACAGTAAAAGAATTGGATT+TGG 0.322823 6_1:-68745060 Msa0891930:CDS
ATTTCACTTGATCGAAAGAT+AGG 0.332402 6_1:-68744877 Msa0891930:CDS
CATAACTTACAAGACAAATA+AGG 0.333702 6_1:-68744431 Msa0891930:CDS
CATTTCATGGATTGTGCACT+TGG 0.334162 6_1:-68744198 Msa0891930:CDS
TAAGCAAATTCTTAAGGTCT+TGG 0.340024 6_1:+68744647 None:intergenic
CATTGTACCGCTTCATGAAA+TGG 0.342244 6_1:+68743396 None:intergenic
ACAGCGGCTGCTCCTTGTTC+TGG 0.346784 6_1:-68742409 Msa0891930:CDS
ACCCATTTATCGACACAATC+TGG 0.348105 6_1:+68745198 None:intergenic
CGACCTTTGGGTGTATTTGT+TGG 0.350273 6_1:-68744403 Msa0891930:CDS
ATTGTCTTGGGAGATGAATC+TGG 0.352705 6_1:-68744742 Msa0891930:CDS
GTTGAGTTTCATTGCTTACT+TGG 0.357841 6_1:+68742908 None:intergenic
TGTGTGTCACTGCACAGTTC+TGG 0.364859 6_1:-68742339 Msa0891930:CDS
AAAGAGTTCGCGATTCCGTT+TGG 0.365271 6_1:-68744131 Msa0891930:CDS
GTTTAATAGACGGAGGTTCC+TGG 0.370085 6_1:-68741283 Msa0891930:intron
ACGCGCTGTGCATCCTGTTC+CGG 0.371872 6_1:+68744906 None:intergenic
AAATAAGGATTATCGACCTT+TGG 0.372421 6_1:-68744416 Msa0891930:CDS
CTTGGCTTGATTGTGTTGCT+TGG 0.381074 6_1:+68742872 None:intergenic
GGAAATAAAAGCAGAATAGA+TGG 0.381196 6_1:-68745021 Msa0891930:CDS
TGCGCACAATTGGAGTGTTT+AGG 0.390563 6_1:-68742311 Msa0891930:CDS
TCGAAAGATAGGGAAATATA+AGG 0.398025 6_1:-68744866 Msa0891930:CDS
CTCTACCCCACCAGCTTGCT+TGG 0.401329 6_1:+68744687 None:intergenic
AGCTCAACATAACCACCTTC+TGG 0.403395 6_1:-68744830 Msa0891930:CDS
TCTGGTGTGGTGCGCACAAT+TGG 0.408841 6_1:-68742321 Msa0891930:CDS
AATAAGGATTATCGACCTTT+GGG 0.412280 6_1:-68744415 Msa0891930:CDS
AGGGAGGCATGGCGGTGAGG+GGG 0.413785 6_1:-68741233 Msa0891930:CDS
GAAGGGAGGCATGGCGGTGA+GGG 0.419623 6_1:-68741235 Msa0891930:CDS
AGAAGGGAGGCATGGCGGTG+AGG 0.425309 6_1:-68741236 Msa0891930:CDS
TGTTATTGGTGTGTGGCTGC+TGG 0.426227 6_1:-68742362 Msa0891930:CDS
TGCCAATAGTGGACCTCACT+TGG 0.426768 6_1:-68745130 Msa0891930:CDS
GCTGGGCTCTTCATAAACTT+TGG 0.428647 6_1:+68743304 None:intergenic
CGGTTTAGTACAATCGTTAC+CGG 0.428762 6_1:-68743042 Msa0891930:intron
ATATAAGACAGAATGCTCTT+AGG 0.429523 6_1:-68744174 Msa0891930:CDS
AATTTATATCTGACTTCAAA+TGG 0.429735 6_1:+68743894 None:intergenic
ATTAATGATTATGGATACTT+TGG 0.430083 6_1:-68744472 Msa0891930:CDS
TCCTGGAGGCATGGCGGCGA+GGG 0.437314 6_1:-68741266 Msa0891930:CDS
TCCCAAGTGAGGTCCACTAT+TGG 0.441740 6_1:+68745128 None:intergenic
GAAATTGGCAAGGGTAAAAG+TGG 0.441981 6_1:-68741438 Msa0891930:CDS
AAGGGAGGCATGGCGGTGAG+GGG 0.442380 6_1:-68741234 Msa0891930:CDS
ATTGTACCGCTTCATGAAAT+GGG 0.445421 6_1:+68743397 None:intergenic
TCAAATAACCACTGAAAAGA+AGG 0.449244 6_1:+68744319 None:intergenic
TCCAGATTGTGTCGATAAAT+GGG 0.451957 6_1:-68745199 Msa0891930:CDS
GCAAAGGAGAGGATGGTAAT+GGG 0.456049 6_1:-68743623 Msa0891930:CDS
TGAAAATCATAAAAGAATGA+TGG 0.460102 6_1:+68744547 None:intergenic
GAAATTACAATGAGTTAACT+TGG 0.462389 6_1:+68742854 None:intergenic
AAAGAAAGTATTGCCAATAG+TGG 0.465982 6_1:-68745141 Msa0891930:CDS
TGCAAAGGAGAGGATGGTAA+TGG 0.465994 6_1:-68743624 Msa0891930:CDS
GACACTGTTGGCAAGGCAAA+AGG 0.467378 6_1:-68743176 Msa0891930:CDS
ATGGACAAGAGATGCAAGGC+TGG 0.468317 6_1:-68743468 Msa0891930:CDS
TGCATCTTGATCTGTTAGAA+TGG 0.469730 6_1:+68744258 None:intergenic
GCGGCGAGGGCGACAGCAGA+AGG 0.471052 6_1:-68741253 Msa0891930:CDS
AGAATTTGCTTAAAACCAAA+AGG 0.473219 6_1:-68744636 Msa0891930:CDS
ATATCAAGATACTTGCATAA+AGG 0.478662 6_1:-68743687 Msa0891930:CDS
ACAAACATCACAAATTGTCT+TGG 0.481510 6_1:-68744755 Msa0891930:CDS
AATGGGCATGGGCTTATGTT+CGG 0.481910 6_1:-68743982 Msa0891930:CDS
TGCATAAAGGAGGTGAAAGA+AGG 0.483547 6_1:-68743674 Msa0891930:CDS
GCCCTCGCCGCCATGCCTCC+AGG 0.486908 6_1:+68741265 None:intergenic
TTGTTTGAAATATGTCATCA+AGG 0.487222 6_1:+68744582 None:intergenic
ATATATTGATGCAACTCTCT+TGG 0.487795 6_1:+68744712 None:intergenic
AACAGGATGCACAGCGCGTA+TGG 0.493774 6_1:-68744902 Msa0891930:CDS
AAAGGGCTGTCAATGCTAAA+CGG 0.496120 6_1:-68743847 Msa0891930:CDS
GATGGTAATGGGAAATCCTA+TGG 0.503211 6_1:-68743612 Msa0891930:CDS
TTGTTGGACTTAATAATCAC+AGG 0.504151 6_1:-68744387 Msa0891930:CDS
ATGCGATCTACTCAATTAAG+TGG 0.512569 6_1:-68743941 Msa0891930:CDS
TCTAGAGCAGAAACCGGAAC+AGG 0.515056 6_1:-68744919 Msa0891930:CDS
GCCTAAATTGAAAATGAAGA+AGG 0.516431 6_1:-68743783 Msa0891930:CDS
TGTTTGTTAGGAGACTTGTA+AGG 0.516731 6_1:-68742741 Msa0891930:intron
CAAACATCACAAATTGTCTT+GGG 0.519857 6_1:-68744754 Msa0891930:CDS
GAAGTAATCAAGGTTGGAGT+GGG 0.524671 6_1:-68743445 Msa0891930:CDS
GCGACAGCAGAAGGGAGGCA+TGG 0.529153 6_1:-68741244 Msa0891930:CDS
AACACTACATACTAAAACGA+TGG 0.530470 6_1:-68743487 Msa0891930:CDS
GCACAATTGGAGTGTTTAGG+GGG 0.533499 6_1:-68742308 Msa0891930:CDS
GCAAGGCTGGGAAGTAATCA+AGG 0.534455 6_1:-68743455 Msa0891930:CDS
CGCACAATTGGAGTGTTTAG+GGG 0.536536 6_1:-68742309 Msa0891930:CDS
TTTCACTTGATCGAAAGATA+GGG 0.538851 6_1:-68744876 Msa0891930:CDS
TGATAATGCAAAGGAGAGGA+TGG 0.539415 6_1:-68743630 Msa0891930:CDS
ATCATATGAACATACTCAGC+TGG 0.541021 6_1:+68744805 None:intergenic
GGCTGGGAAGTAATCAAGGT+TGG 0.541437 6_1:-68743451 Msa0891930:CDS
ATCACTAAAGTACGGTGAAG+TGG 0.545460 6_1:-68744608 Msa0891930:CDS
AAGCTTCTGTTATTGGTGTG+TGG 0.545728 6_1:-68742369 Msa0891930:CDS
TGCATGCTTCAAAATCCAAA+CGG 0.549062 6_1:+68744116 None:intergenic
GCTGCTCCTTGTTCTGGTGG+AGG 0.549405 6_1:-68742403 Msa0891930:CDS
CGGCGAGGGCGACAGCAGAA+GGG 0.550305 6_1:-68741252 Msa0891930:CDS
GTTCGGAAAACATTCACGGC+AGG 0.564623 6_1:-68743965 Msa0891930:CDS
GCGGCTGCTCCTTGTTCTGG+TGG 0.565567 6_1:-68742406 Msa0891930:CDS
TCACTAAAGTACGGTGAAGT+GGG 0.566903 6_1:-68744607 Msa0891930:CDS
GGGAATGCAGTTTGAATCTG+AGG 0.567573 6_1:-68745109 Msa0891930:CDS
TTCCTGGAGGCATGGCGGCG+AGG 0.568691 6_1:-68741267 Msa0891930:CDS
GCCAATAGTGGACCTCACTT+GGG 0.571514 6_1:-68745129 Msa0891930:CDS
GTGCCTCAGATATGTGTCGA+TGG 0.572602 6_1:+68744776 None:intergenic
AACAGATCAAGATGCAGCAA+TGG 0.573110 6_1:-68744251 Msa0891930:CDS
TATATATCCAAGCAAGCTGG+TGG 0.573855 6_1:-68744694 Msa0891930:CDS
CGAGGGCGACAGCAGAAGGG+AGG 0.575218 6_1:-68741249 Msa0891930:CDS
TGAATATTTAGAAATTGGCA+AGG 0.575630 6_1:-68741448 Msa0891930:intron
CAACATAGACACTGTTGGCA+AGG 0.576064 6_1:-68743183 Msa0891930:CDS
TTATGTTCGGAAAACATTCA+CGG 0.576310 6_1:-68743969 Msa0891930:CDS
GTGAATCGACTGGTGTGGAC+TGG 0.576662 6_1:-68741409 Msa0891930:CDS
GACGGAGGTTCCTGGAGGCA+TGG 0.578036 6_1:-68741275 Msa0891930:CDS
GCGGAGTCTAGAGCAGAAAC+CGG 0.578562 6_1:-68744925 Msa0891930:CDS
TGGACAAGAGATGCAAGGCT+GGG 0.580811 6_1:-68743467 Msa0891930:CDS
GTAATCAAGGTTGGAGTGGG+AGG 0.581542 6_1:-68743442 Msa0891930:CDS
AGGAAGCAATCACTAAAGTA+CGG 0.581592 6_1:-68744616 Msa0891930:CDS
CAATATATATCCAAGCAAGC+TGG 0.584632 6_1:-68744697 Msa0891930:CDS
TATATCCAAGCAAGCTGGTG+GGG 0.587725 6_1:-68744692 Msa0891930:CDS
GGATGGAGAATCAAGTGGAA+TGG 0.591122 6_1:-68743246 Msa0891930:CDS
GGAAGTAATCAAGGTTGGAG+TGG 0.603856 6_1:-68743446 Msa0891930:CDS
AAATGTTGAGGAATTTCAAG+AGG 0.603862 6_1:-68743720 Msa0891930:CDS
TGCCTCAGATATGTGTCGAT+GGG 0.606906 6_1:+68744777 None:intergenic
TGCGATCTACTCAATTAAGT+GGG 0.608587 6_1:-68743940 Msa0891930:CDS
TGTCGTAAATTTGAGACACA+TGG 0.610330 6_1:-68743569 Msa0891930:CDS
GAGCTTCCTCCACCAGAACA+AGG 0.610671 6_1:+68742397 None:intergenic
CACAAATTATGATAATGCAA+AGG 0.613820 6_1:-68743639 Msa0891930:CDS
TCTGCTCTAGACTCCGCCGT+CGG 0.614843 6_1:+68744931 None:intergenic
AAAATTCACATGCTTCAAAG+AGG 0.620919 6_1:-68744983 Msa0891930:CDS
GACAACAACATAGACACTGT+TGG 0.621351 6_1:-68743188 Msa0891930:CDS
TCAAGATACTTGCATAAAGG+AGG 0.621392 6_1:-68743684 Msa0891930:CDS
AACGATGGACAAGAGATGCA+AGG 0.621531 6_1:-68743472 Msa0891930:CDS
GCTGGTGGGGTAGAGACTGT+CGG 0.623303 6_1:-68744679 Msa0891930:CDS
GAATATTTAGAAATTGGCAA+GGG 0.629271 6_1:-68741447 Msa0891930:intron
AAATTGGCAAGGGTAAAAGT+GGG 0.630892 6_1:-68741437 Msa0891930:CDS
AAATTCACATGCTTCAAAGA+GGG 0.631197 6_1:-68744982 Msa0891930:CDS
ATATATCCAAGCAAGCTGGT+GGG 0.631572 6_1:-68744693 Msa0891930:CDS
GTCACTGCACAGTTCTGGTG+TGG 0.631768 6_1:-68742334 Msa0891930:CDS
TCGACTGGTGTGGACTGGTG+TGG 0.632789 6_1:-68741404 Msa0891930:CDS
AGTCCAACAAATACACCCAA+AGG 0.632966 6_1:+68744400 None:intergenic
TTGGTCAATTTAAGCAACTG+AGG 0.640782 6_1:+68743365 None:intergenic
CACAATTGGAGTGTTTAGGG+GGG 0.641579 6_1:-68742307 Msa0891930:CDS
GGCAAAAGGCATAAAGACGA+AGG 0.643397 6_1:-68743162 Msa0891930:CDS
ATTATGATAATGCAAAGGAG+AGG 0.652746 6_1:-68743634 Msa0891930:CDS
TCAAACTGCATTCCCAAGTG+AGG 0.657944 6_1:+68745117 None:intergenic
GTGGGTAAGAGTGAATCGAC+TGG 0.664528 6_1:-68741419 Msa0891930:CDS
TGACAAAAGACGTCAACCGA+CGG 0.667263 6_1:-68744947 Msa0891930:CDS
TGTGGAAGAGAAGAGCAGCA+AGG 0.669707 6_1:-68741386 Msa0891930:CDS
CATACTCAGCTGGAACCAGA+AGG 0.670127 6_1:+68744815 None:intergenic
CTGTCGTTATTATTGTACAG+CGG 0.673152 6_1:-68742425 Msa0891930:CDS
TAAGAGTGAATCGACTGGTG+TGG 0.675203 6_1:-68741414 Msa0891930:CDS
ATGCATAGATCTACACGAGG+AGG 0.676939 6_1:-68744098 Msa0891930:CDS
ACTCAGCTGGAACCAGAAGG+TGG 0.678526 6_1:+68744818 None:intergenic
CTCCCATCGACACATATCTG+AGG 0.683683 6_1:-68744779 Msa0891930:CDS
CTGTTATAACAGTGACAGTG+AGG 0.683956 6_1:-68742683 Msa0891930:intron
TATCTGGATGGAGAATCAAG+TGG 0.685001 6_1:-68743251 Msa0891930:CDS
ACAGCAGAAGGGAGGCATGG+CGG 0.695285 6_1:-68741241 Msa0891930:CDS
AATGCAGTTTGAATCTGAGG+CGG 0.700175 6_1:-68745106 Msa0891930:CDS
TAATAGACGGAGGTTCCTGG+AGG 0.702335 6_1:-68741280 Msa0891930:intron
CAAAAGACGTCAACCGACGG+CGG 0.706901 6_1:-68744944 Msa0891930:CDS
GGAGGTTCCTGGAGGCATGG+CGG 0.727679 6_1:-68741272 Msa0891930:CDS
ACAGCACCAGAATCCACACA+AGG 0.733525 6_1:+68742444 None:intergenic
AGCATGCATAGATCTACACG+AGG 0.758431 6_1:-68744101 Msa0891930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCTTTTATGATTTTCAAA+TGG - chr6_1:68741902-68741921 Msa0891930:intron 15.0%
!!! AGCTAATAACAAATATATTT+TGG - chr6_1:68741934-68741953 Msa0891930:intron 15.0%
!!! AATAACAAATATATTTTGGA+CGG - chr6_1:68741938-68741957 Msa0891930:intron 15.0%
!!! TTTTTAAATTGAAGGAAAAA+TGG - chr6_1:68742444-68742463 Msa0891930:CDS 15.0%
!!! TTTTAAATTGAAGGAAAAAT+GGG - chr6_1:68742445-68742464 Msa0891930:CDS 15.0%
!!! AATTTTTTACTCACTTTAAA+AGG - chr6_1:68742579-68742598 Msa0891930:intron 15.0%
!!! ATTTTTTACTCACTTTAAAA+GGG - chr6_1:68742580-68742599 Msa0891930:intron 15.0%
!! AGAAAAGTTAAATAAATGAT+AGG + chr6_1:68743469-68743488 None:intergenic 15.0%
!! AATGATTACAGTAAAAGAAT+TGG - chr6_1:68741378-68741397 Msa0891930:intron 20.0%
!! TGAAAATCATAAAAGAATGA+TGG + chr6_1:68741900-68741919 None:intergenic 20.0%
!! ATTAATGATTATGGATACTT+TGG - chr6_1:68741972-68741991 Msa0891930:intron 20.0%
!!! AAAGTGAATTTTTGAATGAA+TGG - chr6_1:68742369-68742388 Msa0891930:CDS 20.0%
!!! GCATTCAATTTTTAAATTGA+AGG - chr6_1:68742436-68742455 Msa0891930:CDS 20.0%
!! AATTTATATCTGACTTCAAA+TGG + chr6_1:68742553-68742572 None:intergenic 20.0%
!!! TTTGAAGTCAGATATAAATT+TGG - chr6_1:68742553-68742572 Msa0891930:intron 20.0%
!!! ATATCATTAACTATTTTGCT+GGG + chr6_1:68743160-68743179 None:intergenic 20.0%
!! AAAAAGAAAAAATCTCAAGA+CGG - chr6_1:68743382-68743401 Msa0891930:CDS 20.0%
!! ATACAAAAAAATATACGTAC+CGG + chr6_1:68743424-68743443 None:intergenic 20.0%
!!! AGTTTTGATAAATATTGCAA+TGG + chr6_1:68744879-68744898 None:intergenic 20.0%
!! TGTCATGAATATTTAGAAAT+TGG - chr6_1:68744991-68745010 Msa0891930:CDS 20.0%
! TACAGTAAAAGAATTGGATT+TGG - chr6_1:68741384-68741403 Msa0891930:CDS 25.0%
!!! TGGTTTTAAGCAAATTCTTA+AGG + chr6_1:68741806-68741825 None:intergenic 25.0%
!! AGAATTTGCTTAAAACCAAA+AGG - chr6_1:68741808-68741827 Msa0891930:intron 25.0%
!! TTGTTTGAAATATGTCATCA+AGG + chr6_1:68741865-68741884 None:intergenic 25.0%
! CATAACTTACAAGACAAATA+AGG - chr6_1:68742013-68742032 Msa0891930:intron 25.0%
!!! TGAAATTCCTCAACATTTTT+TGG + chr6_1:68742722-68742741 None:intergenic 25.0%
! ATATCAAGATACTTGCATAA+AGG - chr6_1:68742757-68742776 Msa0891930:intron 25.0%
!! CACAAATTATGATAATGCAA+AGG - chr6_1:68742805-68742824 Msa0891930:intron 25.0%
!! CATATCATTAACTATTTTGC+TGG + chr6_1:68743161-68743180 None:intergenic 25.0%
! TATGTTGTCCAAAAAATATC+TGG - chr6_1:68743177-68743196 Msa0891930:CDS 25.0%
! TTCATCATTTGCAATTGATA+TGG + chr6_1:68743233-68743252 None:intergenic 25.0%
! GTAAAAAACGTTATATGTCT+TGG - chr6_1:68743320-68743339 Msa0891930:CDS 25.0%
! GAAATTACAATGAGTTAACT+TGG + chr6_1:68743593-68743612 None:intergenic 25.0%
! AAGTATACAATAAAGGAAAG+AGG + chr6_1:68744253-68744272 None:intergenic 25.0%
! GATGCTAAAGTATACAATAA+AGG + chr6_1:68744260-68744279 None:intergenic 25.0%
!!! AGTATTTTAATCCTTGAGAA+AGG - chr6_1:68744310-68744329 Msa0891930:CDS 25.0%
!! TAGATAATGTTGCTTATGTA+TGG + chr6_1:68744538-68744557 None:intergenic 25.0%
!!! TTAGTTACTAACTTTTTCCA+AGG - chr6_1:68744643-68744662 Msa0891930:CDS 25.0%
!!! GAAATGTTGTTTTATCAGTT+TGG - chr6_1:68744696-68744715 Msa0891930:CDS 25.0%
! ATTTATCAAAACTACTGCTA+TGG - chr6_1:68744886-68744905 Msa0891930:CDS 25.0%
! TGAATATTTAGAAATTGGCA+AGG - chr6_1:68744996-68745015 Msa0891930:CDS 25.0%
! GAATATTTAGAAATTGGCAA+GGG - chr6_1:68744997-68745016 Msa0891930:CDS 25.0%
! TAGATGATGTGTTTAATAGA+CGG - chr6_1:68745151-68745170 Msa0891930:CDS 25.0%
!! AAAGAAAGTATTGCCAATAG+TGG - chr6_1:68741303-68741322 Msa0891930:intron 30.0%
GGAAATAAAAGCAGAATAGA+TGG - chr6_1:68741423-68741442 Msa0891930:CDS 30.0%
AAAATTCACATGCTTCAAAG+AGG - chr6_1:68741461-68741480 Msa0891930:intron 30.0%
AAATTCACATGCTTCAAAGA+GGG - chr6_1:68741462-68741481 Msa0891930:intron 30.0%
ATTTCACTTGATCGAAAGAT+AGG - chr6_1:68741567-68741586 Msa0891930:intron 30.0%
TTTCACTTGATCGAAAGATA+GGG - chr6_1:68741568-68741587 Msa0891930:intron 30.0%
TCGAAAGATAGGGAAATATA+AGG - chr6_1:68741578-68741597 Msa0891930:intron 30.0%
ACAAACATCACAAATTGTCT+TGG - chr6_1:68741689-68741708 Msa0891930:intron 30.0%
CAAACATCACAAATTGTCTT+GGG - chr6_1:68741690-68741709 Msa0891930:intron 30.0%
ATATATTGATGCAACTCTCT+TGG + chr6_1:68741735-68741754 None:intergenic 30.0%
TAAGCAAATTCTTAAGGTCT+TGG + chr6_1:68741800-68741819 None:intergenic 30.0%
GCACAGATGATTAATGATTA+TGG - chr6_1:68741963-68741982 Msa0891930:intron 30.0%
AAATAAGGATTATCGACCTT+TGG - chr6_1:68742028-68742047 Msa0891930:intron 30.0%
AATAAGGATTATCGACCTTT+GGG - chr6_1:68742029-68742048 Msa0891930:intron 30.0%
TTGTTGGACTTAATAATCAC+AGG - chr6_1:68742057-68742076 Msa0891930:intron 30.0%
! AAACAATTCCTTCTTTTCAG+TGG - chr6_1:68742117-68742136 Msa0891930:intron 30.0%
TCAAATAACCACTGAAAAGA+AGG + chr6_1:68742128-68742147 None:intergenic 30.0%
!! TGAAACTTTTCTGAAAGCAA+TGG - chr6_1:68742145-68742164 Msa0891930:intron 30.0%
!! GAAACTTTTCTGAAAGCAAT+GGG - chr6_1:68742146-68742165 Msa0891930:intron 30.0%
! TGATCTGTTAGAATGGTTTT+AGG + chr6_1:68742182-68742201 None:intergenic 30.0%
GTTATGCCAAAAACATTTCA+TGG - chr6_1:68742233-68742252 Msa0891930:intron 30.0%
! CACAATCCATGAAATGTTTT+TGG + chr6_1:68742242-68742261 None:intergenic 30.0%
ATATAAGACAGAATGCTCTT+AGG - chr6_1:68742270-68742289 Msa0891930:intron 30.0%
TTATGTTCGGAAAACATTCA+CGG - chr6_1:68742475-68742494 Msa0891930:intron 30.0%
GCCTAAATTGAAAATGAAGA+AGG - chr6_1:68742661-68742680 Msa0891930:intron 30.0%
! GCCTTCTTCATTTTCAATTT+AGG + chr6_1:68742665-68742684 None:intergenic 30.0%
!!! TTTTGGAGTGTAAACATTTG+CGG + chr6_1:68742705-68742724 None:intergenic 30.0%
TTACACTCCAAAAAATGTTG+AGG - chr6_1:68742712-68742731 Msa0891930:CDS 30.0%
AAATGTTGAGGAATTTCAAG+AGG - chr6_1:68742724-68742743 Msa0891930:CDS 30.0%
! ATTATGATAATGCAAAGGAG+AGG - chr6_1:68742810-68742829 Msa0891930:intron 30.0%
! CAAGAAACTTTTTGTTCCAT+AGG + chr6_1:68742851-68742870 None:intergenic 30.0%
!! TCGTTTTAGTATGTAGTGTT+CGG + chr6_1:68742957-68742976 None:intergenic 30.0%
AACACTACATACTAAAACGA+TGG - chr6_1:68742957-68742976 Msa0891930:intron 30.0%
TTGTCCAAAAAATATCTGGA+TGG - chr6_1:68743181-68743200 Msa0891930:CDS 30.0%
!!! TTCTCCATCCAGATATTTTT+TGG + chr6_1:68743188-68743207 None:intergenic 30.0%
!!! TAACGTTTTTTACTCTTACG+TGG + chr6_1:68743313-68743332 None:intergenic 30.0%
! AACAAACACATTAGAGTACT+TGG + chr6_1:68743652-68743671 None:intergenic 30.0%
! AACAAACACATCAAAGTACT+TGG + chr6_1:68743691-68743710 None:intergenic 30.0%
AGTACTTTGATGTGTTTGTT+AGG - chr6_1:68743691-68743710 Msa0891930:CDS 30.0%
TTTATCAAGAATCATGTGAC+AGG - chr6_1:68743960-68743979 Msa0891930:CDS 30.0%
TCTTTCTAAGCTTCTGTTAT+TGG - chr6_1:68744068-68744087 Msa0891930:CDS 30.0%
AAAGGAAAGAGGAAAACAAT+AGG + chr6_1:68744242-68744261 None:intergenic 30.0%
!! ATTTTAATCCTTGAGAAAGG+TGG - chr6_1:68744313-68744332 Msa0891930:CDS 30.0%
!! TACTAACTTTTTCCAAGGAT+TGG - chr6_1:68744648-68744667 Msa0891930:CDS 30.0%
AGTTCATCATTCATCTATCA+AGG - chr6_1:68744776-68744795 Msa0891930:CDS 30.0%
! TTCCAGATTGTGTCGATAAA+TGG - chr6_1:68741244-68741263 Msa0891930:CDS 35.0%
! TCCAGATTGTGTCGATAAAT+GGG - chr6_1:68741245-68741264 Msa0891930:CDS 35.0%
! CGTGATGAACATTTTGTTCA+TGG + chr6_1:68741281-68741300 None:intergenic 35.0%
TTTGGTATTCGTCGAGAATA+TGG - chr6_1:68741402-68741421 Msa0891930:CDS 35.0%
ATCATATGAACATACTCAGC+TGG + chr6_1:68741642-68741661 None:intergenic 35.0%
CAATATATATCCAAGCAAGC+TGG - chr6_1:68741747-68741766 Msa0891930:intron 35.0%
! CTTTAGTGATTGCTTCCTTT+TGG + chr6_1:68741826-68741845 None:intergenic 35.0%
AGGAAGCAATCACTAAAGTA+CGG - chr6_1:68741828-68741847 Msa0891930:intron 35.0%
!! ACAGGCAAACCATCATTTTT+GGG - chr6_1:68742075-68742094 Msa0891930:intron 35.0%
TGCATCTTGATCTGTTAGAA+TGG + chr6_1:68742189-68742208 None:intergenic 35.0%
! GCGAACTCTTTTGATATAGA+TGG + chr6_1:68742304-68742323 None:intergenic 35.0%
TGCATGCTTCAAAATCCAAA+CGG + chr6_1:68742331-68742350 None:intergenic 35.0%
AATTGAAGGAAAAATGGGCA+TGG - chr6_1:68742450-68742469 Msa0891930:CDS 35.0%
ATTGAAGGAAAAATGGGCAT+GGG - chr6_1:68742451-68742470 Msa0891930:CDS 35.0%
ATGCGATCTACTCAATTAAG+TGG - chr6_1:68742503-68742522 Msa0891930:intron 35.0%
TGCGATCTACTCAATTAAGT+GGG - chr6_1:68742504-68742523 Msa0891930:intron 35.0%
TCAAGATACTTGCATAAAGG+AGG - chr6_1:68742760-68742779 Msa0891930:intron 35.0%
! TGTCGTAAATTTGAGACACA+TGG - chr6_1:68742875-68742894 Msa0891930:CDS 35.0%
!! CGTTTTAGTATGTAGTGTTC+GGG + chr6_1:68742956-68742975 None:intergenic 35.0%
AAAAAGCCCATTTCATGAAG+CGG - chr6_1:68743041-68743060 Msa0891930:intron 35.0%
ATTGTACCGCTTCATGAAAT+GGG + chr6_1:68743050-68743069 None:intergenic 35.0%
TTGGTCAATTTAAGCAACTG+AGG + chr6_1:68743082-68743101 None:intergenic 35.0%
!! ATGAGATTCTGAAGCTCTAT+TGG + chr6_1:68743101-68743120 None:intergenic 35.0%
GTTGAGTTTCATTGCTTACT+TGG + chr6_1:68743539-68743558 None:intergenic 35.0%
!! ACAGTTTTGATGTGTGTGTT+AGG - chr6_1:68743613-68743632 Msa0891930:CDS 35.0%
! TGTTTGTTAGGAGACTTGTA+AGG - chr6_1:68743703-68743722 Msa0891930:CDS 35.0%
!! CTGGTTTAACAATTTGCTAC+TGG - chr6_1:68743786-68743805 Msa0891930:CDS 35.0%
!! CATGTGACAGGTTTTTGTTT+TGG - chr6_1:68743972-68743991 Msa0891930:CDS 35.0%
CTGTCGTTATTATTGTACAG+CGG - chr6_1:68744019-68744038 Msa0891930:CDS 35.0%
! TTGCTGTTATGACAGTGTTT+AGG - chr6_1:68744205-68744224 Msa0891930:CDS 35.0%
TGCTGTTATGACAGTGTTTA+GGG - chr6_1:68744206-68744225 Msa0891930:CDS 35.0%
TCCACAGTAAAAACCCAAAT+AGG + chr6_1:68744291-68744310 None:intergenic 35.0%
AAAGAAGCGGAACATACAAA+AGG + chr6_1:68744367-68744386 None:intergenic 35.0%
ACATTTCATTGTGTCTCTCT+TGG + chr6_1:68744683-68744702 None:intergenic 35.0%
TCATTCATCTATCAAGGTGT+AGG - chr6_1:68744782-68744801 Msa0891930:CDS 35.0%
TTGAAACTTCACAGAGATGA+TGG + chr6_1:68744835-68744854 None:intergenic 35.0%
AAATTGGCAAGGGTAAAAGT+GGG - chr6_1:68745007-68745026 Msa0891930:CDS 35.0%
ATGATGTGTTTAATAGACGG+AGG - chr6_1:68745154-68745173 Msa0891930:CDS 35.0%
ACCCATTTATCGACACAATC+TGG + chr6_1:68741249-68741268 None:intergenic 40.0%
AATGCAGTTTGAATCTGAGG+CGG - chr6_1:68741338-68741357 Msa0891930:intron 40.0%
ATTGTCTTGGGAGATGAATC+TGG - chr6_1:68741702-68741721 Msa0891930:intron 40.0%
TATATATCCAAGCAAGCTGG+TGG - chr6_1:68741750-68741769 Msa0891930:intron 40.0%
ATATATCCAAGCAAGCTGGT+GGG - chr6_1:68741751-68741770 Msa0891930:intron 40.0%
ATCACTAAAGTACGGTGAAG+TGG - chr6_1:68741836-68741855 Msa0891930:intron 40.0%
TCACTAAAGTACGGTGAAGT+GGG - chr6_1:68741837-68741856 Msa0891930:intron 40.0%
AGTCCAACAAATACACCCAA+AGG + chr6_1:68742047-68742066 None:intergenic 40.0%
! CACAGGCAAACCATCATTTT+TGG - chr6_1:68742074-68742093 Msa0891930:intron 40.0%
!! CAGGCAAACCATCATTTTTG+GGG - chr6_1:68742076-68742095 Msa0891930:intron 40.0%
CAATGTTGCCCCAAAAATGA+TGG + chr6_1:68742087-68742106 None:intergenic 40.0%
!! ACTTTTCTGAAAGCAATGGG+TGG - chr6_1:68742149-68742168 Msa0891930:intron 40.0%
AACAGATCAAGATGCAGCAA+TGG - chr6_1:68742193-68742212 Msa0891930:intron 40.0%
! CATTTCATGGATTGTGCACT+TGG - chr6_1:68742246-68742265 Msa0891930:intron 40.0%
AAAGGGCTGTCAATGCTAAA+CGG - chr6_1:68742597-68742616 Msa0891930:intron 40.0%
TGCATAAAGGAGGTGAAAGA+AGG - chr6_1:68742770-68742789 Msa0891930:intron 40.0%
! TGATAATGCAAAGGAGAGGA+TGG - chr6_1:68742814-68742833 Msa0891930:intron 40.0%
!! GATGGTAATGGGAAATCCTA+TGG - chr6_1:68742832-68742851 Msa0891930:intron 40.0%
GAAGTAATCAAGGTTGGAGT+GGG - chr6_1:68742999-68743018 Msa0891930:intron 40.0%
!! GCTTCATGAAATGGGCTTTT+TGG + chr6_1:68743042-68743061 None:intergenic 40.0%
CATTGTACCGCTTCATGAAA+TGG + chr6_1:68743051-68743070 None:intergenic 40.0%
TATCTGGATGGAGAATCAAG+TGG - chr6_1:68743193-68743212 Msa0891930:CDS 40.0%
GACAACAACATAGACACTGT+TGG - chr6_1:68743256-68743275 Msa0891930:CDS 40.0%
CGGTTTAGTACAATCGTTAC+CGG - chr6_1:68743402-68743421 Msa0891930:CDS 40.0%
CTGTTATAACAGTGACAGTG+AGG - chr6_1:68743761-68743780 Msa0891930:CDS 40.0%
!!! GTGACAGGTTTTTGTTTTGG+CGG - chr6_1:68743975-68743994 Msa0891930:CDS 40.0%
AAGCTTCTGTTATTGGTGTG+TGG - chr6_1:68744075-68744094 Msa0891930:CDS 40.0%
GCTGTTATGACAGTGTTTAG+GGG - chr6_1:68744207-68744226 Msa0891930:CDS 40.0%
AGCATCATGCTTAGCCTATT+TGG - chr6_1:68744274-68744293 Msa0891930:CDS 40.0%
GCATCATGCTTAGCCTATTT+GGG - chr6_1:68744275-68744294 Msa0891930:CDS 40.0%
!! GCCTATTTGGGTTTTTACTG+TGG - chr6_1:68744287-68744306 Msa0891930:CDS 40.0%
CCAAAAAACCACCTTTCTCA+AGG + chr6_1:68744324-68744343 None:intergenic 40.0%
!!! CCTTGAGAAAGGTGGTTTTT+TGG - chr6_1:68744321-68744340 Msa0891930:CDS 40.0%
!! ATACAAAAGGTGCCGCATAT+AGG + chr6_1:68744354-68744373 None:intergenic 40.0%
GCGGATAAAAACGAAAGAAG+CGG + chr6_1:68744380-68744399 None:intergenic 40.0%
!! CTTTCGTTTTTATCCGCAAG+CGG - chr6_1:68744383-68744402 Msa0891930:CDS 40.0%
!!! ACTTTTTCCAAGGATTGGCT+TGG - chr6_1:68744653-68744672 Msa0891930:CDS 40.0%
!!! CTTTTTCCAAGGATTGGCTT+GGG - chr6_1:68744654-68744673 Msa0891930:CDS 40.0%
GAAATTGGCAAGGGTAAAAG+TGG - chr6_1:68745006-68745025 Msa0891930:CDS 40.0%
!!! GCAAGGTGTTTTAGTATGTG+AGG - chr6_1:68745075-68745094 Msa0891930:CDS 40.0%
!!! AGGTGTTTTAGTATGTGAGG+AGG - chr6_1:68745078-68745097 Msa0891930:CDS 40.0%
!!! GGTGTTTTAGTATGTGAGGA+GGG - chr6_1:68745079-68745098 Msa0891930:CDS 40.0%
TCAAACTGCATTCCCAAGTG+AGG + chr6_1:68741330-68741349 None:intergenic 45.0%
GGGAATGCAGTTTGAATCTG+AGG - chr6_1:68741335-68741354 Msa0891930:intron 45.0%
TGACAAAAGACGTCAACCGA+CGG - chr6_1:68741497-68741516 Msa0891930:intron 45.0%
AGCTCAACATAACCACCTTC+TGG - chr6_1:68741614-68741633 Msa0891930:intron 45.0%
! TGCCTCAGATATGTGTCGAT+GGG + chr6_1:68741670-68741689 None:intergenic 45.0%
TATATCCAAGCAAGCTGGTG+GGG - chr6_1:68741752-68741771 Msa0891930:intron 45.0%
CGACCTTTGGGTGTATTTGT+TGG - chr6_1:68742041-68742060 Msa0891930:intron 45.0%
AAAGAGTTCGCGATTCCGTT+TGG - chr6_1:68742313-68742332 Msa0891930:CDS 45.0%
AGCATGCATAGATCTACACG+AGG - chr6_1:68742343-68742362 Msa0891930:CDS 45.0%
ATGCATAGATCTACACGAGG+AGG - chr6_1:68742346-68742365 Msa0891930:CDS 45.0%
AATGGGCATGGGCTTATGTT+CGG - chr6_1:68742462-68742481 Msa0891930:CDS 45.0%
TGCAAAGGAGAGGATGGTAA+TGG - chr6_1:68742820-68742839 Msa0891930:intron 45.0%
GCAAAGGAGAGGATGGTAAT+GGG - chr6_1:68742821-68742840 Msa0891930:intron 45.0%
AACGATGGACAAGAGATGCA+AGG - chr6_1:68742972-68742991 Msa0891930:intron 45.0%
GGAAGTAATCAAGGTTGGAG+TGG - chr6_1:68742998-68743017 Msa0891930:intron 45.0%
GCTGGGCTCTTCATAAACTT+TGG + chr6_1:68743143-68743162 None:intergenic 45.0%
GGATGGAGAATCAAGTGGAA+TGG - chr6_1:68743198-68743217 Msa0891930:CDS 45.0%
CAACATAGACACTGTTGGCA+AGG - chr6_1:68743261-68743280 Msa0891930:CDS 45.0%
! GGCAAAAGGCATAAAGACGA+AGG - chr6_1:68743282-68743301 Msa0891930:CDS 45.0%
! CTTGGCTTGATTGTGTTGCT+TGG + chr6_1:68743575-68743594 None:intergenic 45.0%
TAACAGTGACAGTGAGGTAC+TGG - chr6_1:68743767-68743786 Msa0891930:CDS 45.0%
! TGCGCACAATTGGAGTGTTT+AGG - chr6_1:68744133-68744152 Msa0891930:CDS 45.0%
! GCGCACAATTGGAGTGTTTA+GGG - chr6_1:68744134-68744153 Msa0891930:CDS 45.0%
! CGCACAATTGGAGTGTTTAG+GGG - chr6_1:68744135-68744154 Msa0891930:CDS 45.0%
! GCACAATTGGAGTGTTTAGG+GGG - chr6_1:68744136-68744155 Msa0891930:CDS 45.0%
! CACAATTGGAGTGTTTAGGG+GGG - chr6_1:68744137-68744156 Msa0891930:CDS 45.0%
!! TTTGGTTCTCCGCCTATATG+CGG - chr6_1:68744339-68744358 Msa0891930:CDS 45.0%
! GTTTTTATCCGCAAGCGGTA+CGG - chr6_1:68744388-68744407 Msa0891930:CDS 45.0%
TAAGAGTGAATCGACTGGTG+TGG - chr6_1:68745030-68745049 Msa0891930:CDS 45.0%
!! GTTTTAGTATGTGAGGAGGG+CGG - chr6_1:68745082-68745101 Msa0891930:CDS 45.0%
GTTTAATAGACGGAGGTTCC+TGG - chr6_1:68745161-68745180 Msa0891930:CDS 45.0%
!! TGCCAATAGTGGACCTCACT+TGG - chr6_1:68741314-68741333 Msa0891930:intron 50.0%
!! GCCAATAGTGGACCTCACTT+GGG - chr6_1:68741315-68741334 Msa0891930:intron 50.0%
TCCCAAGTGAGGTCCACTAT+TGG + chr6_1:68741319-68741338 None:intergenic 50.0%
! TCTAGAGCAGAAACCGGAAC+AGG - chr6_1:68741525-68741544 Msa0891930:intron 50.0%
CATACTCAGCTGGAACCAGA+AGG + chr6_1:68741632-68741651 None:intergenic 50.0%
CTCCCATCGACACATATCTG+AGG - chr6_1:68741665-68741684 Msa0891930:intron 50.0%
GTGCCTCAGATATGTGTCGA+TGG + chr6_1:68741671-68741690 None:intergenic 50.0%
GTTCGGAAAACATTCACGGC+AGG - chr6_1:68742479-68742498 Msa0891930:intron 50.0%
ATGGACAAGAGATGCAAGGC+TGG - chr6_1:68742976-68742995 Msa0891930:intron 50.0%
TGGACAAGAGATGCAAGGCT+GGG - chr6_1:68742977-68742996 Msa0891930:intron 50.0%
GCAAGGCTGGGAAGTAATCA+AGG - chr6_1:68742989-68743008 Msa0891930:intron 50.0%
GGCTGGGAAGTAATCAAGGT+TGG - chr6_1:68742993-68743012 Msa0891930:intron 50.0%
GTAATCAAGGTTGGAGTGGG+AGG - chr6_1:68743002-68743021 Msa0891930:intron 50.0%
GACACTGTTGGCAAGGCAAA+AGG - chr6_1:68743268-68743287 Msa0891930:CDS 50.0%
!!! TGTTTTGGCGGATCCTTGTG+TGG - chr6_1:68743987-68744006 Msa0891930:CDS 50.0%
ACAGCACCAGAATCCACACA+AGG + chr6_1:68744003-68744022 None:intergenic 50.0%
TGTTATTGGTGTGTGGCTGC+TGG - chr6_1:68744082-68744101 Msa0891930:CDS 50.0%
! TGTGTGTCACTGCACAGTTC+TGG - chr6_1:68744105-68744124 Msa0891930:CDS 50.0%
! AGGCTTTGGTATGCTCAGCT+GGG - chr6_1:68744169-68744188 Msa0891930:CDS 50.0%
! TATGCTCAGCTGGGACTAGT+TGG - chr6_1:68744178-68744197 Msa0891930:CDS 50.0%
!! ATGCTCAGCTGGGACTAGTT+GGG - chr6_1:68744179-68744198 Msa0891930:CDS 50.0%
!! CAAAAGGTGCCGCATATAGG+CGG + chr6_1:68744351-68744370 None:intergenic 50.0%
TGGTTTCCCAAGCCAATCCT+TGG + chr6_1:68744663-68744682 None:intergenic 50.0%
GTGGGTAAGAGTGAATCGAC+TGG - chr6_1:68745025-68745044 Msa0891930:CDS 50.0%
TGTGGAAGAGAAGAGCAGCA+AGG - chr6_1:68745058-68745077 Msa0891930:CDS 50.0%
TAATAGACGGAGGTTCCTGG+AGG - chr6_1:68745164-68745183 Msa0891930:CDS 50.0%
CAAAAGACGTCAACCGACGG+CGG - chr6_1:68741500-68741519 Msa0891930:intron 55.0%
! GCGGAGTCTAGAGCAGAAAC+CGG - chr6_1:68741519-68741538 Msa0891930:intron 55.0%
AACAGGATGCACAGCGCGTA+TGG - chr6_1:68741542-68741561 Msa0891930:intron 55.0%
ACTCAGCTGGAACCAGAAGG+TGG + chr6_1:68741629-68741648 None:intergenic 55.0%
GCGGATCCTTGTGTGGATTC+TGG - chr6_1:68743994-68744013 Msa0891930:CDS 55.0%
GAGCTTCCTCCACCAGAACA+AGG + chr6_1:68744050-68744069 None:intergenic 55.0%
! GTCACTGCACAGTTCTGGTG+TGG - chr6_1:68744110-68744129 Msa0891930:CDS 55.0%
TCTGGTGTGGTGCGCACAAT+TGG - chr6_1:68744123-68744142 Msa0891930:CDS 55.0%
! GAGGCTTTGGTATGCTCAGC+TGG - chr6_1:68744168-68744187 Msa0891930:CDS 55.0%
ACAAGAAGCCGTACCGCTTG+CGG + chr6_1:68744399-68744418 None:intergenic 55.0%
GTGAATCGACTGGTGTGGAC+TGG - chr6_1:68745035-68745054 Msa0891930:CDS 55.0%
! TCTGCTCTAGACTCCGCCGT+CGG + chr6_1:68741516-68741535 None:intergenic 60.0%
ACGCGCTGTGCATCCTGTTC+CGG + chr6_1:68741541-68741560 None:intergenic 60.0%
! CTCTACCCCACCAGCTTGCT+TGG + chr6_1:68741760-68741779 None:intergenic 60.0%
GCTGGTGGGGTAGAGACTGT+CGG - chr6_1:68741765-68741784 Msa0891930:intron 60.0%
ACAGCGGCTGCTCCTTGTTC+TGG - chr6_1:68744035-68744054 Msa0891930:CDS 60.0%
GCTGCTCCTTGTTCTGGTGG+AGG - chr6_1:68744041-68744060 Msa0891930:CDS 60.0%
GTTTAGGGGGGCTGTTAGCG+AGG - chr6_1:68744149-68744168 Msa0891930:CDS 60.0%
!! TCGACTGGTGTGGACTGGTG+TGG - chr6_1:68745040-68745059 Msa0891930:CDS 60.0%
ATGTGAGGAGGGCGGTGAGA+CGG - chr6_1:68745090-68745109 Msa0891930:CDS 60.0%
AGAGGGGCGGTGATAGGAAG+AGG - chr6_1:68745127-68745146 Msa0891930:CDS 60.0%
GAGGGGCGGTGATAGGAAGA+GGG - chr6_1:68745128-68745147 Msa0891930:CDS 60.0%
! ACAGCAGAAGGGAGGCATGG+CGG - chr6_1:68745203-68745222 Msa0891930:CDS 60.0%
GCGGCTGCTCCTTGTTCTGG+TGG - chr6_1:68744038-68744057 Msa0891930:CDS 65.0%
GGGGGCTGTTAGCGAGGCTT+TGG - chr6_1:68744155-68744174 Msa0891930:CDS 65.0%
GACGGAGGTTCCTGGAGGCA+TGG - chr6_1:68745169-68745188 Msa0891930:CDS 65.0%
! GGAGGTTCCTGGAGGCATGG+CGG - chr6_1:68745172-68745191 Msa0891930:CDS 65.0%
GCGACAGCAGAAGGGAGGCA+TGG - chr6_1:68745200-68745219 Msa0891930:CDS 65.0%
! AGAAGGGAGGCATGGCGGTG+AGG - chr6_1:68745208-68745227 Msa0891930:CDS 65.0%
! GAAGGGAGGCATGGCGGTGA+GGG - chr6_1:68745209-68745228 Msa0891930:CDS 65.0%
! AAGGGAGGCATGGCGGTGAG+GGG - chr6_1:68745210-68745229 Msa0891930:CDS 65.0%
TGAGGAGGGCGGTGAGACGG+CGG - chr6_1:68745093-68745112 Msa0891930:CDS 70.0%
GCGGTGAGACGGCGGTGAGA+CGG - chr6_1:68745101-68745120 Msa0891930:CDS 70.0%
CGGTGAGACGGCGGTGAGAC+GGG - chr6_1:68745102-68745121 Msa0891930:CDS 70.0%
GGTGAGACGGCGGTGAGACG+GGG - chr6_1:68745103-68745122 Msa0891930:CDS 70.0%
GTGAGACGGCGGTGAGACGG+GGG - chr6_1:68745104-68745123 Msa0891930:CDS 70.0%
! TTCCTGGAGGCATGGCGGCG+AGG - chr6_1:68745177-68745196 Msa0891930:CDS 70.0%
! TCCTGGAGGCATGGCGGCGA+GGG - chr6_1:68745178-68745197 Msa0891930:CDS 70.0%
CGGCGAGGGCGACAGCAGAA+GGG - chr6_1:68745192-68745211 Msa0891930:CDS 70.0%
CGAGGGCGACAGCAGAAGGG+AGG - chr6_1:68745195-68745214 Msa0891930:CDS 70.0%
! AGGGAGGCATGGCGGTGAGG+GGG - chr6_1:68745211-68745230 Msa0891930:CDS 70.0%
ACGGCGGTGAGACGGGGGAG+AGG - chr6_1:68745109-68745128 Msa0891930:CDS 75.0%
CGGCGGTGAGACGGGGGAGA+GGG - chr6_1:68745110-68745129 Msa0891930:CDS 75.0%
GGCGGTGAGACGGGGGAGAG+GGG - chr6_1:68745111-68745130 Msa0891930:CDS 75.0%
GGTGAGACGGGGGAGAGGGG+CGG - chr6_1:68745114-68745133 Msa0891930:CDS 75.0%
CGGGGGAGAGGGGCGGTGAT+AGG - chr6_1:68745121-68745140 Msa0891930:CDS 75.0%
GCGGCGAGGGCGACAGCAGA+AGG - chr6_1:68745191-68745210 Msa0891930:CDS 75.0%
! GCCCTCGCCGCCATGCCTCC+AGG + chr6_1:68745182-68745201 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 68741228 68745238 68741228 ID=Msa0891930;Name=Msa0891930
chr6_1 mRNA 68741228 68745238 68741228 ID=Msa0891930-mRNA-1;Parent=Msa0891930;Name=Msa0891930-mRNA-1;_AED=0.22;_eAED=0.25;_QI=0|0|0|0.33|1|1|6|0|904
chr6_1 exon 68743043 68745238 68743043 ID=Msa0891930-mRNA-1:exon:12160;Parent=Msa0891930-mRNA-1
chr6_1 exon 68742862 68742965 68742862 ID=Msa0891930-mRNA-1:exon:12159;Parent=Msa0891930-mRNA-1
chr6_1 exon 68742684 68742753 68742684 ID=Msa0891930-mRNA-1:exon:12158;Parent=Msa0891930-mRNA-1
chr6_1 exon 68742290 68742484 68742290 ID=Msa0891930-mRNA-1:exon:12157;Parent=Msa0891930-mRNA-1
chr6_1 exon 68741379 68741459 68741379 ID=Msa0891930-mRNA-1:exon:12156;Parent=Msa0891930-mRNA-1
chr6_1 exon 68741228 68741296 68741228 ID=Msa0891930-mRNA-1:exon:12155;Parent=Msa0891930-mRNA-1
chr6_1 CDS 68743043 68745238 68743043 ID=Msa0891930-mRNA-1:cds;Parent=Msa0891930-mRNA-1
chr6_1 CDS 68742862 68742965 68742862 ID=Msa0891930-mRNA-1:cds;Parent=Msa0891930-mRNA-1
chr6_1 CDS 68742684 68742753 68742684 ID=Msa0891930-mRNA-1:cds;Parent=Msa0891930-mRNA-1
chr6_1 CDS 68742290 68742484 68742290 ID=Msa0891930-mRNA-1:cds;Parent=Msa0891930-mRNA-1
chr6_1 CDS 68741379 68741459 68741379 ID=Msa0891930-mRNA-1:cds;Parent=Msa0891930-mRNA-1
chr6_1 CDS 68741228 68741296 68741228 ID=Msa0891930-mRNA-1:cds;Parent=Msa0891930-mRNA-1
Gene Sequence

>Msa0891930

ATGGATTCATCATCAATTCCAGATTGTGTCGATAAATGGGTGAACAATATCCATGAACAAAATGTTCATCACGAGAAAGAAAGTATTGCCAATAGTGGACCTCACTTGGGAATGCAGTTTGAATCTGAGGCGGTAGCATATGAATTTTATAATGATTACAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAATAGATGGAGTTTTGACTTCGAGAAAATTCACATGCTTCAAAGAGGGTGCGAAAGTTGTTGACAAAAGACGTCAACCGACGGCGGAGTCTAGAGCAGAAACCGGAACAGGATGCACAGCGCGTATGGTTATTTCACTTGATCGAAAGATAGGGAAATATAAGGTTGTTGACTTTGAAGCTCAACATAACCACCTTCTGGTTCCAGCTGAGTATGTTCATATGATTCGCTCCCATCGACACATATCTGAGGCACAAACATCACAAATTGTCTTGGGAGATGAATCTGGCTTAAGACCAAGAGAGTTGCATCAATATATATCCAAGCAAGCTGGTGGGGTAGAGACTGTCGGTTTTACAAGCCAAGACCTTAAGAATTTGCTTAAAACCAAAAGGAAGCAATCACTAAAGTACGGTGAAGTGGGTGCCTTGATGACATATTTCAAACAAGAAAGTGAAAATCCATCATTCTTTTATGATTTTCAAATGGATGTTGATGAGCTAATAACAAATATATTTTGGACGGATGCACAGATGATTAATGATTATGGATACTTTGGTGATATCATCACTTTTGACATAACTTACAAGACAAATAAGGATTATCGACCTTTGGGTGTATTTGTTGGACTTAATAATCACAGGCAAACCATCATTTTTGGGGCAACATTGTTATATGATGAAACAATTCCTTCTTTTCAGTGGTTATTTGAAACTTTTCTGAAAGCAATGGGTGGAGAAAAGCCTAAAACCATTCTAACAGATCAAGATGCAGCAATGGCTAAAGCTATTTCTTTAGTTATGCCAAAAACATTTCATGGATTGTGCACTTGGCATATAAGACAGAATGCTCTTAGGCATGTGAACCATCTATATCAAAAGAGTTCGCGATTCCGTTTGGATTTTGAAGCATGCATAGATCTACACGAGGAGGAAAGTGAATTTTTGAATGAATGGAATTCTTTACTTGTTGATCATAATGTTTTGAAAGATTCGTTGTTGCATTCAATTTTTAAATTGAAGGAAAAATGGGCATGGGCTTATGTTCGGAAAACATTCACGGCAGGTATGCGATCTACTCAATTAAGTGGGAGCTTTAATGCTGATTTGAAAAACCATTTGAAGTCAGATATAAATTTGGTACAATTTTTTACTCACTTTAAAAGGGCTGTCAATGCTAAACGGAACAATGAGTCAGAAGCAGAATATGATTCAAGACATAAGCTGCCTAAATTGAAAATGAAGAAGGCTCGTATGCTCTTACAAGCCGCAAATGTTTACACTCCAAAAAATGTTGAGGAATTTCAAGAGGAATATGAAGAATATCAAGATACTTGCATAAAGGAGGTGAAAGAAGGTTTGTATGTTGTCACAAATTATGATAATGCAAAGGAGAGGATGGTAATGGGAAATCCTATGGAACAAAAAGTTTCTTGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGTAGTCATGATTTGAAAGTTTTAGATGTCATGAACATTAAGTTACTTCCCGAACACTACATACTAAAACGATGGACAAGAGATGCAAGGCTGGGAAGTAATCAAGGTTGGAGTGGGAGGCATGTTGAATTAGACCAAAAAGCCCATTTCATGAAGCGGTACAATGAGTTATGTCCTCAGTTGCTTAAATTGACCAATAGAGCTTCAGAATCTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGCCCAGCAAAATAGTTAATGATATGTTGTCCAAAAAATATCTGGATGGAGAATCAAGTGGAATGGTTCATGTATCCATATCAATTGCAAATGATGAAGTCGACAACAACATAGACACTGTTGGCAAGGCAAAAGGCATAAAGACGAAGGATTGTCCACGTAAGAGTAAAAAACGTTATATGTCTTGGCTTGAAAAAGAACGTAAAAAGAAAAAGATGCGCTCAGAAAAAAAGAAAAAATCTCAAGACGGTTTAGTACAATCGTTACCGCAATCAAGCAACACAATTCAAGCTACACAATCAAGCCAAGTAAGCAATGAAACTCAACATCAAGATACAATCCAAGCAACACAATCAAGCCAAGTTAACTCATTGAGACTTGTAAGGTTTAAGAATTTTGATGTAAAATATCACAAATCTGTCTGTTATAACAGTGACAGTGAGGTTTTTGTTTTGGCGGATCCTTGTGTGGATTCTGGTGCTGTCGTTATTATTGTACAGCGGCTGCTCCTTGTTCTGGTGGAGGAAGCTCTTTCTAAGCTTCTGTTATTGGTGTGTGGCTGCTGGTGTGTGTCACTGCACAGTTCTGGTGTGGTGCGCACAATTGGAGTGTTTAGGGGGGCTGTTAGCGAGGCTTTGAAATTGGCAAGGGTAAAAGTGGGTAAGAGTGAATCGACTGGTGTGGACTGGTGTGGAAGAGAAGAGCAGCAAGGTGTTTTAACGGAGGTTCCTGGAGGCATGGCGGCGAGGGCGACAGCAGAAGGGAGGCATGGCGGTGAGGGGGATTGA
Protein sequence

>Msa0891930

MDSSSIPDCVDKWVNNIHEQNVHHEKESIANSGPHLGMQFESEAVAYEFYNDYSKRIGFGIRREYGNKSRIDGVLTSRKFTCFKEGAKVVDKRRQPTAESRAETGTGCTARMVISLDRKIGKYKVVDFEAQHNHLLVPAEYVHMIRSHRHISEAQTSQIVLGDESGLRPRELHQYISKQAGGVETVGFTSQDLKNLLKTKRKQSLKYGEVGALMTYFKQESENPSFFYDFQMDVDELITNIFWTDAQMINDYGYFGDIITFDITYKTNKDYRPLGVFVGLNNHRQTIIFGATLLYDETIPSFQWLFETFLKAMGGEKPKTILTDQDAAMAKAISLVMPKTFHGLCTWHIRQNALRHVNHLYQKSSRFRLDFEACIDLHEEESEFLNEWNSLLVDHNVLKDSLLHSIFKLKEKWAWAYVRKTFTAGMRSTQLSGSFNADLKNHLKSDINLVQFFTHFKRAVNAKRNNESEAEYDSRHKLPKLKMKKARMLLQAANVYTPKNVEEFQEEYEEYQDTCIKEVKEGLYVVTNYDNAKERMVMGNPMEQKVSCDCRKFETHGILCSHDLKVLDVMNIKLLPEHYILKRWTRDARLGSNQGWSGRHVELDQKAHFMKRYNELCPQLLKLTNRASESHETYTFLSKVYEEPSKIVNDMLSKKYLDGESSGMVHVSISIANDEVDNNIDTVGKAKGIKTKDCPRKSKKRYMSWLEKERKKKKMRSEKKKKSQDGLVQSLPQSSNTIQATQSSQVSNETQHQDTIQATQSSQVNSLRLVRFKNFDVKYHKSVCYNSDSEVFVLADPCVDSGAVVIIVQRLLLVLVEEALSKLLLLVCGCWCVSLHSSGVVRTIGVFRGAVSEALKLARVKVGKSESTGVDWCGREEQQGVLTEVPGGMAARATAEGRHGGEGD*