Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0893940 | KAG4937911.1 | 73.565 | 575 | 104 | 8 | 14 | 550 | 12 | 576 | 0.0 | 840 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0893940 | sp|Q9SY66|FRS11_ARATH | 41.176 | 238 | 114 | 7 | 50 | 267 | 21 | 252 | 2.29e-43 | 167 |
| Msa0893940 | sp|Q9SY66|FRS11_ARATH | 41.477 | 176 | 99 | 2 | 271 | 444 | 429 | 602 | 5.62e-37 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0893940 | A0A445GCJ8 | 65.167 | 600 | 136 | 12 | 14 | 550 | 12 | 601 | 0.0 | 744 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0893940 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0893940 | MtrunA17_Chr1g0198761 | 35.238 | 315 | 148 | 7 | 271 | 536 | 433 | 740 | 1.13e-50 | 186 |
| Msa0893940 | MtrunA17_Chr1g0198761 | 33.600 | 250 | 136 | 8 | 78 | 299 | 46 | 293 | 1.90e-29 | 123 |
| Msa0893940 | MtrunA17_Chr2g0276841 | 42.925 | 212 | 101 | 5 | 78 | 271 | 69 | 278 | 4.31e-44 | 166 |
| Msa0893940 | MtrunA17_Chr2g0276841 | 44.068 | 177 | 93 | 4 | 271 | 444 | 451 | 624 | 1.45e-39 | 153 |
| Msa0893940 | MtrunA17_Chr2g0294841 | 39.062 | 256 | 120 | 7 | 57 | 278 | 11 | 264 | 3.81e-39 | 152 |
| Msa0893940 | MtrunA17_Chr2g0294841 | 36.932 | 176 | 108 | 2 | 271 | 444 | 429 | 603 | 3.23e-31 | 129 |
| Msa0893940 | MtrunA17_Chr5g0431001 | 36.000 | 250 | 130 | 8 | 78 | 299 | 60 | 307 | 7.36e-32 | 129 |
| Msa0893940 | MtrunA17_Chr5g0431001 | 70.270 | 37 | 11 | 0 | 271 | 307 | 447 | 483 | 3.66e-11 | 65.5 |
| Msa0893940 | MtrunA17_Chr2g0290391 | 35.510 | 245 | 120 | 9 | 56 | 271 | 32 | 267 | 5.75e-30 | 124 |
| Msa0893940 | MtrunA17_Chr2g0290391 | 35.795 | 176 | 104 | 3 | 271 | 444 | 440 | 608 | 2.80e-29 | 122 |
| Msa0893940 | MtrunA17_Chr1g0202341 | 36.066 | 183 | 112 | 1 | 271 | 448 | 426 | 608 | 3.44e-26 | 113 |
| Msa0893940 | MtrunA17_Chr5g0448761 | 33.152 | 184 | 110 | 4 | 274 | 448 | 445 | 624 | 4.39e-23 | 103 |
| Msa0893940 | MtrunA17_Chr5g0448511 | 29.218 | 243 | 149 | 6 | 252 | 480 | 186 | 419 | 1.10e-22 | 101 |
| Msa0893940 | MtrunA17_Chr5g0440451 | 31.148 | 183 | 119 | 4 | 271 | 448 | 423 | 603 | 2.40e-21 | 98.6 |
| Msa0893940 | MtrunA17_Chr3g0080351 | 30.366 | 191 | 115 | 3 | 269 | 448 | 439 | 622 | 3.18e-20 | 94.7 |
| Msa0893940 | MtrunA17_Chr1g0207551 | 31.414 | 191 | 126 | 3 | 262 | 448 | 399 | 588 | 8.05e-20 | 93.6 |
| Msa0893940 | MtrunA17_Chr5g0441581 | 35.829 | 187 | 108 | 6 | 269 | 444 | 393 | 578 | 1.52e-19 | 92.4 |
| Msa0893940 | MtrunA17_Chr3g0118611 | 30.978 | 184 | 118 | 4 | 271 | 448 | 320 | 500 | 2.37e-19 | 92.0 |
| Msa0893940 | MtrunA17_Chr2g0296781 | 34.146 | 205 | 109 | 9 | 271 | 452 | 367 | 568 | 2.99e-19 | 91.7 |
| Msa0893940 | MtrunA17_Chr6g0477261 | 32.353 | 204 | 114 | 7 | 271 | 452 | 256 | 457 | 1.87e-18 | 89.0 |
| Msa0893940 | MtrunA17_Chr2g0329031 | 28.814 | 177 | 123 | 2 | 271 | 444 | 380 | 556 | 1.35e-16 | 83.2 |
| Msa0893940 | MtrunA17_Chr2g0291581 | 33.161 | 193 | 107 | 7 | 269 | 444 | 429 | 616 | 8.10e-16 | 80.9 |
| Msa0893940 | MtrunA17_Chr1g0196541 | 30.808 | 198 | 112 | 7 | 264 | 444 | 362 | 551 | 2.71e-14 | 75.9 |
| Msa0893940 | MtrunA17_Chr2g0324011 | 27.807 | 187 | 130 | 3 | 269 | 450 | 383 | 569 | 5.69e-14 | 74.7 |
| Msa0893940 | MtrunA17_Chr2g0308841 | 26.374 | 182 | 128 | 3 | 269 | 444 | 41 | 222 | 9.45e-13 | 68.6 |
| Msa0893940 | MtrunA17_Chr2g0287651 | 25.134 | 187 | 128 | 4 | 271 | 448 | 448 | 631 | 7.20e-12 | 68.6 |
| Msa0893940 | MtrunA17_Chr2g0287061 | 26.344 | 186 | 128 | 4 | 269 | 448 | 518 | 700 | 1.64e-11 | 67.0 |
| Msa0893940 | MtrunA17_Chr3g0082121 | 30.435 | 184 | 121 | 4 | 274 | 453 | 414 | 594 | 2.38e-11 | 66.6 |
| Msa0893940 | MtrunA17_Chr3g0081281 | 38.667 | 75 | 43 | 1 | 206 | 277 | 1 | 75 | 4.65e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0893940 | AT1G10240.2 | 41.176 | 238 | 114 | 7 | 50 | 267 | 21 | 252 | 2.32e-44 | 167 |
| Msa0893940 | AT1G10240.2 | 41.477 | 176 | 99 | 2 | 271 | 444 | 429 | 602 | 5.71e-38 | 149 |
| Msa0893940 | AT1G10240.1 | 41.176 | 238 | 114 | 7 | 50 | 267 | 21 | 252 | 2.32e-44 | 167 |
| Msa0893940 | AT1G10240.1 | 41.477 | 176 | 99 | 2 | 271 | 444 | 429 | 602 | 5.71e-38 | 149 |
| Msa0893940 | AT5G28530.1 | 34.728 | 239 | 126 | 6 | 60 | 271 | 36 | 271 | 1.15e-30 | 127 |
| Msa0893940 | AT5G28530.1 | 30.000 | 180 | 113 | 4 | 271 | 444 | 444 | 616 | 7.58e-19 | 90.9 |
| Msa0893940 | AT5G28530.3 | 34.728 | 239 | 126 | 6 | 60 | 271 | 36 | 271 | 1.17e-30 | 127 |
| Msa0893940 | AT5G28530.3 | 30.114 | 176 | 114 | 3 | 271 | 444 | 444 | 612 | 3.17e-20 | 95.1 |
| Msa0893940 | AT5G28530.2 | 34.728 | 239 | 126 | 6 | 60 | 271 | 36 | 271 | 1.17e-30 | 127 |
| Msa0893940 | AT5G28530.2 | 30.114 | 176 | 114 | 3 | 271 | 444 | 444 | 612 | 3.17e-20 | 95.1 |
| Msa0893940 | AT5G28530.4 | 33.465 | 254 | 138 | 7 | 60 | 285 | 36 | 286 | 1.17e-30 | 127 |
| Msa0893940 | AT5G28530.4 | 30.000 | 180 | 113 | 4 | 271 | 444 | 381 | 553 | 3.44e-19 | 91.7 |
| Msa0893940 | AT5G28530.5 | 33.465 | 254 | 138 | 7 | 60 | 285 | 36 | 286 | 1.40e-30 | 126 |
| Msa0893940 | AT5G28530.5 | 30.114 | 176 | 114 | 3 | 271 | 444 | 381 | 549 | 1.74e-20 | 95.9 |
| Msa0893940 | AT4G38170.1 | 32.212 | 208 | 134 | 3 | 271 | 472 | 216 | 422 | 4.97e-26 | 112 |
| Msa0893940 | AT4G38180.1 | 33.333 | 204 | 121 | 5 | 271 | 462 | 451 | 651 | 7.39e-24 | 106 |
| Msa0893940 | AT3G22170.2 | 32.990 | 194 | 121 | 5 | 261 | 448 | 427 | 617 | 2.71e-20 | 95.5 |
| Msa0893940 | AT3G22170.1 | 32.990 | 194 | 121 | 5 | 261 | 448 | 427 | 617 | 2.71e-20 | 95.5 |
| Msa0893940 | AT3G06250.2 | 33.166 | 199 | 122 | 6 | 269 | 460 | 522 | 716 | 2.60e-18 | 89.4 |
| Msa0893940 | AT3G06250.1 | 33.166 | 199 | 122 | 6 | 269 | 460 | 522 | 716 | 2.60e-18 | 89.4 |
| Msa0893940 | AT3G06250.3 | 33.166 | 199 | 122 | 6 | 269 | 460 | 522 | 716 | 2.60e-18 | 89.4 |
| Msa0893940 | AT4G19990.3 | 30.851 | 188 | 120 | 3 | 269 | 448 | 373 | 558 | 2.11e-17 | 86.3 |
| Msa0893940 | AT4G19990.4 | 30.851 | 188 | 120 | 3 | 269 | 448 | 373 | 558 | 2.11e-17 | 86.3 |
| Msa0893940 | AT4G19990.1 | 30.851 | 188 | 120 | 3 | 269 | 448 | 316 | 501 | 2.11e-17 | 86.3 |
| Msa0893940 | AT4G19990.5 | 30.851 | 188 | 120 | 3 | 269 | 448 | 373 | 558 | 2.12e-17 | 86.3 |
| Msa0893940 | AT4G19990.7 | 30.851 | 188 | 120 | 3 | 269 | 448 | 387 | 572 | 2.16e-17 | 86.3 |
| Msa0893940 | AT4G19990.6 | 30.851 | 188 | 120 | 3 | 269 | 448 | 373 | 558 | 2.57e-17 | 86.3 |
| Msa0893940 | AT4G19990.2 | 30.851 | 188 | 120 | 3 | 269 | 448 | 373 | 558 | 2.57e-17 | 86.3 |
| Msa0893940 | AT1G76320.2 | 28.333 | 180 | 123 | 3 | 269 | 444 | 362 | 539 | 3.19e-17 | 85.9 |
| Msa0893940 | AT1G76320.1 | 28.333 | 180 | 123 | 3 | 269 | 444 | 362 | 539 | 3.31e-17 | 85.5 |
| Msa0893940 | AT1G76320.4 | 28.333 | 180 | 123 | 3 | 269 | 444 | 362 | 539 | 3.31e-17 | 85.5 |
| Msa0893940 | AT1G76320.3 | 28.333 | 180 | 123 | 3 | 269 | 444 | 362 | 539 | 3.78e-17 | 85.5 |
| Msa0893940 | AT4G15090.1 | 29.834 | 181 | 121 | 4 | 269 | 444 | 425 | 604 | 1.38e-16 | 84.0 |
| Msa0893940 | AT4G15090.5 | 29.834 | 181 | 121 | 4 | 269 | 444 | 425 | 604 | 1.38e-16 | 84.0 |
| Msa0893940 | AT4G15090.3 | 29.834 | 181 | 121 | 4 | 269 | 444 | 425 | 604 | 1.38e-16 | 84.0 |
| Msa0893940 | AT4G15090.2 | 29.834 | 181 | 121 | 4 | 269 | 444 | 425 | 604 | 1.38e-16 | 84.0 |
| Msa0893940 | AT4G15090.4 | 29.834 | 181 | 121 | 4 | 269 | 444 | 425 | 604 | 1.38e-16 | 84.0 |
| Msa0893940 | AT1G52520.1 | 28.947 | 190 | 120 | 5 | 269 | 444 | 445 | 633 | 1.39e-16 | 83.6 |
| Msa0893940 | AT5G18960.2 | 30.851 | 188 | 120 | 5 | 269 | 450 | 546 | 729 | 1.51e-15 | 80.5 |
| Msa0893940 | AT5G18960.1 | 30.851 | 188 | 120 | 5 | 269 | 450 | 546 | 729 | 1.51e-15 | 80.5 |
| Msa0893940 | AT2G27110.2 | 29.891 | 184 | 120 | 4 | 271 | 448 | 398 | 578 | 2.72e-15 | 79.7 |
| Msa0893940 | AT2G27110.1 | 29.891 | 184 | 120 | 4 | 271 | 448 | 398 | 578 | 2.72e-15 | 79.7 |
| Msa0893940 | AT2G27110.3 | 29.891 | 184 | 120 | 4 | 271 | 448 | 253 | 433 | 3.85e-15 | 79.0 |
| Msa0893940 | AT2G32250.5 | 25.281 | 178 | 129 | 2 | 271 | 444 | 371 | 548 | 2.37e-11 | 67.0 |
| Msa0893940 | AT2G32250.2 | 25.281 | 178 | 129 | 2 | 271 | 444 | 371 | 548 | 2.37e-11 | 67.0 |
| Msa0893940 | AT2G32250.4 | 25.281 | 178 | 129 | 2 | 271 | 444 | 371 | 548 | 2.37e-11 | 67.0 |
| Msa0893940 | AT2G32250.6 | 25.281 | 178 | 129 | 2 | 271 | 444 | 371 | 548 | 2.53e-11 | 67.0 |
| Msa0893940 | AT2G32250.1 | 25.281 | 178 | 129 | 2 | 271 | 444 | 371 | 548 | 2.53e-11 | 67.0 |
| Msa0893940 | AT2G32250.3 | 25.281 | 178 | 129 | 2 | 271 | 444 | 371 | 548 | 2.53e-11 | 67.0 |
| Msa0893940 | AT1G80010.1 | 26.702 | 191 | 117 | 7 | 271 | 444 | 472 | 656 | 2.72e-11 | 67.0 |
| Msa0893940 | AT2G32250.8 | 25.281 | 178 | 129 | 2 | 271 | 444 | 330 | 507 | 2.80e-11 | 67.0 |
| Msa0893940 | AT2G32250.7 | 25.281 | 178 | 129 | 2 | 271 | 444 | 330 | 507 | 2.90e-11 | 66.6 |
Find 76 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATTGTGTTCCCTTTAT+TGG | 0.123228 | 6_1:-78921798 | Msa0893940:CDS |
| CGAATGTCCCTTTCAATAAA+TGG | 0.156974 | 6_1:+78921315 | None:intergenic |
| TATCTTCGTGATTATTTCTT+TGG | 0.163692 | 6_1:-78920508 | Msa0893940:CDS |
| CGACCTTTAGGTTTGGATTT+TGG | 0.167927 | 6_1:+78919604 | None:intergenic |
| GAAGATATTGCTTTAGAAAA+TGG | 0.185026 | 6_1:-78921966 | Msa0893940:CDS |
| CCTAAACGACGGCGACTTAA+AGG | 0.199216 | 6_1:-78919580 | Msa0893940:CDS |
| GTTGTAAGCTCTTTGAATTT+TGG | 0.244484 | 6_1:-78919828 | Msa0893940:CDS |
| CCTTTAAGTCGCCGTCGTTT+AGG | 0.263372 | 6_1:+78919580 | None:intergenic |
| GCAGAAAACATGCAGTGTTT+TGG | 0.283471 | 6_1:-78919873 | Msa0893940:CDS |
| TATTCAATTGATCATGAAAA+TGG | 0.285695 | 6_1:-78919934 | Msa0893940:CDS |
| TAAATGTGTACCTAAACTTT+TGG | 0.293742 | 6_1:+78919412 | None:intergenic |
| CGTCGTTTAGGACGACCTTT+AGG | 0.309582 | 6_1:+78919592 | None:intergenic |
| AATTCTTCTTCCAAAAGTTT+AGG | 0.313803 | 6_1:-78919422 | None:intergenic |
| AAATGGTTGTTCAGAAATAA+TGG | 0.340072 | 6_1:-78921949 | Msa0893940:CDS |
| TGATGGTTCCATGAATTTCA+TGG | 0.362135 | 6_1:+78921626 | None:intergenic |
| TTTAACCGACTTTGTAAAAC+AGG | 0.371626 | 6_1:-78920413 | Msa0893940:intron |
| CGTGATGGCAAGTAGTTAGA+AGG | 0.372743 | 6_1:+78919748 | None:intergenic |
| ATTTGAGAGAAGTGATGTTA+TGG | 0.378695 | 6_1:-78921265 | Msa0893940:CDS |
| GTATGAAATCAGAAACTATT+GGG | 0.418531 | 6_1:-78920539 | Msa0893940:intron |
| GTAGATTCACAAACAAGAAT+GGG | 0.422387 | 6_1:-78921695 | Msa0893940:CDS |
| TGTATGAAATCAGAAACTAT+TGG | 0.424688 | 6_1:-78920540 | Msa0893940:intron |
| TTTAGGACGACCTTTAGGTT+TGG | 0.425449 | 6_1:+78919597 | None:intergenic |
| CTTCGTGATTATTTCTTTGG+TGG | 0.427885 | 6_1:-78920505 | Msa0893940:CDS |
| TTTGCCTTCTTTCCCTTTAG+CGG | 0.431590 | 6_1:+78919484 | None:intergenic |
| TAGAGCCTCCATGAAATTCA+TGG | 0.438357 | 6_1:-78921634 | Msa0893940:CDS |
| CATTGTCACATGTCCGCTAA+AGG | 0.446736 | 6_1:-78919497 | Msa0893940:CDS |
| TTCGTGATTATTTCTTTGGT+GGG | 0.450960 | 6_1:-78920504 | Msa0893940:CDS |
| CGGTATTATAAAGAGGTTAA+CGG | 0.457669 | 6_1:-78919895 | Msa0893940:CDS |
| ATTAATACTCTCCGATCTTC+CGG | 0.458636 | 6_1:+78920471 | None:intergenic |
| GGTAGATTCACAAACAAGAA+TGG | 0.461401 | 6_1:-78921696 | Msa0893940:CDS |
| ATTGTCACATGTCCGCTAAA+GGG | 0.468125 | 6_1:-78919496 | Msa0893940:CDS |
| AATGAAGAAGGTGATAAAGA+TGG | 0.470337 | 6_1:-78921834 | Msa0893940:intron |
| TAACCTTCCATTTATTGAAA+GGG | 0.475583 | 6_1:-78921322 | Msa0893940:CDS |
| ATGGTAATGTGTTTGTGCTT+CGG | 0.482748 | 6_1:-78919915 | Msa0893940:CDS |
| GTAGATGATAAAGAACATAA+TGG | 0.487034 | 6_1:-78922051 | None:intergenic |
| GTAACCTTCCATTTATTGAA+AGG | 0.500947 | 6_1:-78921323 | Msa0893940:CDS |
| TTTAGTGAAGGACTTGTTAG+TGG | 0.507871 | 6_1:-78921916 | Msa0893940:CDS |
| GAAGGTGATAAAGATGGGTC+TGG | 0.517282 | 6_1:-78921828 | Msa0893940:intron |
| GTTGTAACTCGCCACTCAGT+TGG | 0.517634 | 6_1:+78921519 | None:intergenic |
| ATGTCCGCTAAAGGGAAAGA+AGG | 0.522488 | 6_1:-78919488 | Msa0893940:CDS |
| TGTGCTTCGGTATTATAAAG+AGG | 0.524446 | 6_1:-78919902 | Msa0893940:CDS |
| AGAGCAGAGAAATAGAGAAT+CGG | 0.530469 | 6_1:-78921601 | Msa0893940:CDS |
| TTGTTAGTGGTTGATAACAT+AGG | 0.538715 | 6_1:-78921903 | Msa0893940:CDS |
| CCTCCAAAATCCAAACCTAA+AGG | 0.542127 | 6_1:-78919607 | Msa0893940:CDS |
| ACAAGAATGGGGTTATGAGC+AGG | 0.542872 | 6_1:-78921683 | Msa0893940:CDS |
| ATGAAGAAGGTGATAAAGAT+GGG | 0.545139 | 6_1:-78921833 | Msa0893940:intron |
| GATGACAACCACCGGAAGAT+CGG | 0.546284 | 6_1:-78920482 | Msa0893940:CDS |
| AGTTTCAAGAGGAGTTTGAA+AGG | 0.562176 | 6_1:-78919966 | Msa0893940:CDS |
| TCAGAGATAGTTCGATAAGC+CGG | 0.569841 | 6_1:+78921441 | None:intergenic |
| TCACATAACATGAACACTTG+TGG | 0.569949 | 6_1:-78919544 | Msa0893940:CDS |
| CTGATTGGTTTAGCAAACCA+TGG | 0.574779 | 6_1:+78921478 | None:intergenic |
| CTTCTAACTACTTGCCATCA+CGG | 0.575563 | 6_1:-78919747 | Msa0893940:CDS |
| TGTCCCTTTCAATAAATGGA+AGG | 0.578784 | 6_1:+78921319 | None:intergenic |
| ATACACTTGATGAGGAAAGA+AGG | 0.579288 | 6_1:-78921185 | Msa0893940:intron |
| TTTGGTGGGATGACAACCAC+CGG | 0.581206 | 6_1:-78920490 | Msa0893940:CDS |
| AAGAGATATGCTTATCAACA+AGG | 0.596535 | 6_1:-78921735 | Msa0893940:CDS |
| AAACGACGGCGACTTAAAGG+TGG | 0.596721 | 6_1:-78919577 | Msa0893940:CDS |
| TTTGTTGTTGAACATAACCA+TGG | 0.597979 | 6_1:-78921495 | Msa0893940:CDS |
| ATGATCAATTGAATATTGAG+CGG | 0.600490 | 6_1:+78919942 | None:intergenic |
| AATACTCTCCGATCTTCCGG+TGG | 0.600856 | 6_1:+78920474 | None:intergenic |
| GGTTGAGCATGATTTAGTGA+AGG | 0.607141 | 6_1:-78921928 | Msa0893940:CDS |
| CTATGAGCTTGCTCTTGTAG+AGG | 0.612240 | 6_1:+78920015 | None:intergenic |
| ATATGCATATACACTTGATG+AGG | 0.621673 | 6_1:-78921193 | Msa0893940:CDS |
| GTGATGGCAAGTAGTTAGAA+GGG | 0.633198 | 6_1:+78919749 | None:intergenic |
| TGTGGATTATGCAAAGGTGA+AGG | 0.636366 | 6_1:-78919526 | Msa0893940:CDS |
| GGATCTTCTGAAGTATTGCG+AGG | 0.640419 | 6_1:-78921244 | Msa0893940:CDS |
| AGAGATATGCTTATCAACAA+GGG | 0.641950 | 6_1:-78921734 | Msa0893940:CDS |
| ATGATATATTTCCAACTGAG+TGG | 0.655744 | 6_1:-78921530 | Msa0893940:CDS |
| GCTTGAAGCAACCACCGTGA+TGG | 0.657308 | 6_1:+78919733 | None:intergenic |
| ATAATGTTAGAGAAGTGCAA+AGG | 0.660770 | 6_1:-78920060 | Msa0893940:CDS |
| TGGTTTGCTAAACCAATCAG+AGG | 0.671864 | 6_1:-78921475 | Msa0893940:CDS |
| TAGATTCACAAACAAGAATG+GGG | 0.673558 | 6_1:-78921694 | Msa0893940:CDS |
| AACACTTGTGGATTATGCAA+AGG | 0.684041 | 6_1:-78919532 | Msa0893940:CDS |
| CTAAAGGTCGTCCTAAACGA+CGG | 0.691619 | 6_1:-78919591 | Msa0893940:CDS |
| TGGTTCCATGAATTTCATGG+AGG | 0.699375 | 6_1:+78921629 | None:intergenic |
| CTAACTACTTGCCATCACGG+TGG | 0.742949 | 6_1:-78919744 | Msa0893940:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CGTATTTTTTTATTAAAAAA+AGG | - | chr6_1:78920065-78920084 | Msa0893940:CDS | 10.0% |
| !!! | ATTTTTTTATTAAAAAAAGG+TGG | - | chr6_1:78920068-78920087 | Msa0893940:CDS | 10.0% |
| !!! | TTAAAATAATTTTGTTTCAT+GGG | + | chr6_1:78921259-78921278 | None:intergenic | 10.0% |
| !!! | ATTAAAATAATTTTGTTTCA+TGG | + | chr6_1:78921260-78921279 | None:intergenic | 10.0% |
| !!! | TTTAATTTTAAAAGTTAATC+CGG | - | chr6_1:78921280-78921299 | Msa0893940:CDS | 10.0% |
| !!! | ATCAAAACTTATTTTATTGT+TGG | + | chr6_1:78919466-78919485 | None:intergenic | 15.0% |
| !! | AATATTGTAACAAATGAAGA+AGG | - | chr6_1:78919624-78919643 | Msa0893940:CDS | 20.0% |
| !! | AGAAAAATTTGACCAATAAA+GGG | + | chr6_1:78919687-78919706 | None:intergenic | 20.0% |
| !! | AAGAAAAATTTGACCAATAA+AGG | + | chr6_1:78919688-78919707 | None:intergenic | 20.0% |
| !! | TTAATAAAAAAATACGCTCA+CGG | + | chr6_1:78920061-78920080 | None:intergenic | 20.0% |
| !! | AACGCATATGTATATAAAAA+AGG | + | chr6_1:78920574-78920593 | None:intergenic | 20.0% |
| !!! | TTTTTATGAGTTGTATAAGT+TGG | - | chr6_1:78920826-78920845 | Msa0893940:intron | 20.0% |
| !!! | ATTTCTGTAGACAATTTTTT+CGG | - | chr6_1:78921223-78921242 | Msa0893940:CDS | 20.0% |
| !! | TATTCAATTGATCATGAAAA+TGG | - | chr6_1:78921536-78921555 | Msa0893940:CDS | 20.0% |
| !!! | GAAGATATTGCTTTAGAAAA+TGG | - | chr6_1:78919504-78919523 | Msa0893940:CDS | 25.0% |
| ! | AAATGGTTGTTCAGAAATAA+TGG | - | chr6_1:78919521-78919540 | Msa0893940:CDS | 25.0% |
| ! | ATTAAAAAAAGGTGGTCTTT+CGG | - | chr6_1:78920076-78920095 | Msa0893940:CDS | 25.0% |
| ! | TAACCTTCCATTTATTGAAA+GGG | - | chr6_1:78920148-78920167 | Msa0893940:intron | 25.0% |
| ! | TTTGATTGGTACCAAAAATA+TGG | - | chr6_1:78920341-78920360 | Msa0893940:intron | 25.0% |
| ! | TGTATTTGATACTACATACA+AGG | - | chr6_1:78920373-78920392 | Msa0893940:intron | 25.0% |
| ! | AATTCTTATGAAATGCCATT+TGG | - | chr6_1:78920398-78920417 | Msa0893940:intron | 25.0% |
| !!! | TAACTTTCAAGTTTAGTTCT+TGG | - | chr6_1:78920759-78920778 | Msa0893940:intron | 25.0% |
| ! | TTGTATTTAGCAACAACATT+TGG | + | chr6_1:78920881-78920900 | None:intergenic | 25.0% |
| ! | TGTATGAAATCAGAAACTAT+TGG | - | chr6_1:78920930-78920949 | Msa0893940:intron | 25.0% |
| ! | GTATGAAATCAGAAACTATT+GGG | - | chr6_1:78920931-78920950 | Msa0893940:intron | 25.0% |
| !! | TATCTTCGTGATTATTTCTT+TGG | - | chr6_1:78920962-78920981 | Msa0893940:intron | 25.0% |
| !! | TTCTTTTCAAAAGTTTCAAG+AGG | - | chr6_1:78921493-78921512 | Msa0893940:CDS | 25.0% |
| ! | ATGATCAATTGAATATTGAG+CGG | + | chr6_1:78921531-78921550 | None:intergenic | 25.0% |
| !! | TTGTAAGCTCTTTGAATTTT+GGG | - | chr6_1:78921643-78921662 | Msa0893940:intron | 25.0% |
| !!! | TAAACTTTTGGAAGAAGAAT+TGG | + | chr6_1:78922049-78922068 | None:intergenic | 25.0% |
| !! | TTGTTAGTGGTTGATAACAT+AGG | - | chr6_1:78919567-78919586 | Msa0893940:CDS | 30.0% |
| AATGAAGAAGGTGATAAAGA+TGG | - | chr6_1:78919636-78919655 | Msa0893940:CDS | 30.0% | |
| ATGAAGAAGGTGATAAAGAT+GGG | - | chr6_1:78919637-78919656 | Msa0893940:CDS | 30.0% | |
| ACAAATTGTGTTCCCTTTAT+TGG | - | chr6_1:78919672-78919691 | Msa0893940:CDS | 30.0% | |
| AAGAGATATGCTTATCAACA+AGG | - | chr6_1:78919735-78919754 | Msa0893940:CDS | 30.0% | |
| AGAGATATGCTTATCAACAA+GGG | - | chr6_1:78919736-78919755 | Msa0893940:CDS | 30.0% | |
| CAAGGGTTCTCAATAAAAAA+AGG | - | chr6_1:78919753-78919772 | Msa0893940:CDS | 30.0% | |
| GTAGATTCACAAACAAGAAT+GGG | - | chr6_1:78919775-78919794 | Msa0893940:CDS | 30.0% | |
| TAGATTCACAAACAAGAATG+GGG | - | chr6_1:78919776-78919795 | Msa0893940:CDS | 30.0% | |
| ATGATATATTTCCAACTGAG+TGG | - | chr6_1:78919940-78919959 | Msa0893940:CDS | 30.0% | |
| !! | TTTGTTGTTGAACATAACCA+TGG | - | chr6_1:78919975-78919994 | Msa0893940:CDS | 30.0% |
| GTAACCTTCCATTTATTGAA+AGG | - | chr6_1:78920147-78920166 | Msa0893940:intron | 30.0% | |
| ATTTGAGAGAAGTGATGTTA+TGG | - | chr6_1:78920205-78920224 | Msa0893940:intron | 30.0% | |
| ATATGCATATACACTTGATG+AGG | - | chr6_1:78920277-78920296 | Msa0893940:intron | 30.0% | |
| !! | AATGCCATTTGGAATTTTTG+TGG | - | chr6_1:78920409-78920428 | Msa0893940:intron | 30.0% |
| !! | ATGCCATTTGGAATTTTTGT+GGG | - | chr6_1:78920410-78920429 | Msa0893940:intron | 30.0% |
| ATACCCACAAAAATTCCAAA+TGG | + | chr6_1:78920416-78920435 | None:intergenic | 30.0% | |
| TTTGTGGGTATGAATAATCA+TGG | - | chr6_1:78920425-78920444 | Msa0893940:CDS | 30.0% | |
| TGATCGGTTAATATAGTCTT+TGG | + | chr6_1:78920668-78920687 | None:intergenic | 30.0% | |
| TATTAACCGATCAAGATCTA+TGG | - | chr6_1:78920675-78920694 | Msa0893940:intron | 30.0% | |
| TTTCATCCATAGATCTTGAT+CGG | + | chr6_1:78920684-78920703 | None:intergenic | 30.0% | |
| TTCGAGACAAATATGCAAAA+TGG | - | chr6_1:78920795-78920814 | Msa0893940:intron | 30.0% | |
| CAGTCAAATAAACATGTGAA+AGG | - | chr6_1:78920905-78920924 | Msa0893940:intron | 30.0% | |
| ! | TTCGTGATTATTTCTTTGGT+GGG | - | chr6_1:78920966-78920985 | Msa0893940:intron | 30.0% |
| ! | TTTAACCGACTTTGTAAAAC+AGG | - | chr6_1:78921057-78921076 | Msa0893940:intron | 30.0% |
| ! | TCATACCTGTTTTACAAAGT+CGG | + | chr6_1:78921065-78921084 | None:intergenic | 30.0% |
| TAGTGTATTCTTGAAAATGC+TGG | + | chr6_1:78921196-78921215 | None:intergenic | 30.0% | |
| ! | ATAATGTTAGAGAAGTGCAA+AGG | - | chr6_1:78921410-78921429 | Msa0893940:intron | 30.0% |
| CGGTATTATAAAGAGGTTAA+CGG | - | chr6_1:78921575-78921594 | Msa0893940:CDS | 30.0% | |
| ! | GTTGTAAGCTCTTTGAATTT+TGG | - | chr6_1:78921642-78921661 | Msa0893940:intron | 30.0% |
| ! | TGTAAGCTCTTTGAATTTTG+GGG | - | chr6_1:78921644-78921663 | Msa0893940:intron | 30.0% |
| !! | TTTAGTGAAGGACTTGTTAG+TGG | - | chr6_1:78919554-78919573 | Msa0893940:CDS | 35.0% |
| GGTAGATTCACAAACAAGAA+TGG | - | chr6_1:78919774-78919793 | Msa0893940:CDS | 35.0% | |
| !!! | GATTTTTTTTGCCATCGTGA+AGG | - | chr6_1:78919813-78919832 | Msa0893940:CDS | 35.0% |
| TGATGGTTCCATGAATTTCA+TGG | + | chr6_1:78919847-78919866 | None:intergenic | 35.0% | |
| !! | CTATTTCTCTGCTCTTTTGA+TGG | + | chr6_1:78919864-78919883 | None:intergenic | 35.0% |
| AGAGCAGAGAAATAGAGAAT+CGG | - | chr6_1:78919869-78919888 | Msa0893940:CDS | 35.0% | |
| CTAGAGAAAAATGTGAAGCA+TGG | - | chr6_1:78920125-78920144 | Msa0893940:intron | 35.0% | |
| TGTCCCTTTCAATAAATGGA+AGG | + | chr6_1:78920154-78920173 | None:intergenic | 35.0% | |
| CGAATGTCCCTTTCAATAAA+TGG | + | chr6_1:78920158-78920177 | None:intergenic | 35.0% | |
| ATACACTTGATGAGGAAAGA+AGG | - | chr6_1:78920285-78920304 | Msa0893940:intron | 35.0% | |
| !!! | GAAGGTTGGAGCATATTTTT+TGG | - | chr6_1:78920303-78920322 | Msa0893940:intron | 35.0% |
| !!! | GGAGCATATTTTTTGGTCAT+CGG | - | chr6_1:78920310-78920329 | Msa0893940:intron | 35.0% |
| ! | CAACAACATCGCCATATTTT+TGG | + | chr6_1:78920355-78920374 | None:intergenic | 35.0% |
| ! | TTTTTGGATGTGCACTCTTA+CGG | - | chr6_1:78920459-78920478 | Msa0893940:CDS | 35.0% |
| TCGGTTAATATAGTCTTTGG+TGG | + | chr6_1:78920665-78920684 | None:intergenic | 35.0% | |
| CAAAACACAGTTTCTGCATA+TGG | - | chr6_1:78920732-78920751 | Msa0893940:intron | 35.0% | |
| TGCATATTTGTCTCGAAGTA+TGG | + | chr6_1:78920792-78920811 | None:intergenic | 35.0% | |
| GTGAAGAATTTGAGCATCAA+TGG | - | chr6_1:78920855-78920874 | Msa0893940:intron | 35.0% | |
| ! | CTTCGTGATTATTTCTTTGG+TGG | - | chr6_1:78920965-78920984 | Msa0893940:intron | 35.0% |
| ATTAATACTCTCCGATCTTC+CGG | + | chr6_1:78921002-78921021 | None:intergenic | 35.0% | |
| GGAGGAAGAAAATGAGAAAT+GGG | + | chr6_1:78921175-78921194 | None:intergenic | 35.0% | |
| TGGAGGAAGAAAATGAGAAA+TGG | + | chr6_1:78921176-78921195 | None:intergenic | 35.0% | |
| TGTATTCTTGAAAATGCTGG+AGG | + | chr6_1:78921193-78921212 | None:intergenic | 35.0% | |
| GTCTACAGAAATAGTAACAC+CGG | + | chr6_1:78921302-78921321 | None:intergenic | 35.0% | |
| AGTTTCAAGAGGAGTTTGAA+AGG | - | chr6_1:78921504-78921523 | Msa0893940:CDS | 35.0% | |
| ! | ATGGTAATGTGTTTGTGCTT+CGG | - | chr6_1:78921555-78921574 | Msa0893940:CDS | 35.0% |
| TGTGCTTCGGTATTATAAAG+AGG | - | chr6_1:78921568-78921587 | Msa0893940:CDS | 35.0% | |
| ! | CAGAAAACATGCAGTGTTTT+GGG | - | chr6_1:78921598-78921617 | Msa0893940:CDS | 35.0% |
| ! | GTAAGCTCTTTGAATTTTGG+GGG | - | chr6_1:78921645-78921664 | Msa0893940:intron | 35.0% |
| TCACATAACATGAACACTTG+TGG | - | chr6_1:78921926-78921945 | Msa0893940:CDS | 35.0% | |
| ! | AACACTTGTGGATTATGCAA+AGG | - | chr6_1:78921938-78921957 | Msa0893940:CDS | 35.0% |
| GGTTGAGCATGATTTAGTGA+AGG | - | chr6_1:78919542-78919561 | Msa0893940:CDS | 40.0% | |
| TAGAGCCTCCATGAAATTCA+TGG | - | chr6_1:78919836-78919855 | Msa0893940:CDS | 40.0% | |
| TGGTTCCATGAATTTCATGG+AGG | + | chr6_1:78919844-78919863 | None:intergenic | 40.0% | |
| !! | CTGATTGGTTTAGCAAACCA+TGG | + | chr6_1:78919995-78920014 | None:intergenic | 40.0% |
| !! | TGGTTTGCTAAACCAATCAG+AGG | - | chr6_1:78919995-78920014 | Msa0893940:CDS | 40.0% |
| GTAAAAAACGCACCTCTGAT+TGG | + | chr6_1:78920010-78920029 | None:intergenic | 40.0% | |
| !!! | ATCAGAGGTGCGTTTTTTAC+CGG | - | chr6_1:78920010-78920029 | Msa0893940:CDS | 40.0% |
| TCAGAGATAGTTCGATAAGC+CGG | + | chr6_1:78920032-78920051 | None:intergenic | 40.0% | |
| ACTTGATGAGGAAAGAAGGT+TGG | - | chr6_1:78920289-78920308 | Msa0893940:intron | 40.0% | |
| !!! | CATCGGCTTCTTGTTTTGAT+TGG | - | chr6_1:78920327-78920346 | Msa0893940:intron | 40.0% |
| ! | CATGGAAAGACCGTACTTTT+TGG | - | chr6_1:78920443-78920462 | Msa0893940:CDS | 40.0% |
| GAGTGCACATCCAAAAAGTA+CGG | + | chr6_1:78920456-78920475 | None:intergenic | 40.0% | |
| AAACTGTGTCTGCATTTCGT+TGG | - | chr6_1:78920486-78920505 | Msa0893940:CDS | 40.0% | |
| ! | TGCATTTCGTTGGCTAATGA+AGG | - | chr6_1:78920496-78920515 | Msa0893940:CDS | 40.0% |
| GGAGAGTTTCTTAAGGATGA+TGG | + | chr6_1:78921132-78921151 | None:intergenic | 40.0% | |
| GACATGTACAAAGTTTCACG+GGG | + | chr6_1:78921153-78921172 | None:intergenic | 40.0% | |
| GGACATGTACAAAGTTTCAC+GGG | + | chr6_1:78921154-78921173 | None:intergenic | 40.0% | |
| GGGACATGTACAAAGTTTCA+CGG | + | chr6_1:78921155-78921174 | None:intergenic | 40.0% | |
| ! | GCAGAAAACATGCAGTGTTT+TGG | - | chr6_1:78921597-78921616 | Msa0893940:CDS | 40.0% |
| !!! | AAACATGCAGTGTTTTGGGA+TGG | - | chr6_1:78921602-78921621 | Msa0893940:CDS | 40.0% |
| GTGATGGCAAGTAGTTAGAA+GGG | + | chr6_1:78921724-78921743 | None:intergenic | 40.0% | |
| CTTCTAACTACTTGCCATCA+CGG | - | chr6_1:78921723-78921742 | Msa0893940:CDS | 40.0% | |
| !! | CCTTTAGGTTTGGATTTTGG+AGG | + | chr6_1:78921866-78921885 | None:intergenic | 40.0% |
| CCTCCAAAATCCAAACCTAA+AGG | - | chr6_1:78921863-78921882 | Msa0893940:intron | 40.0% | |
| !! | CGACCTTTAGGTTTGGATTT+TGG | + | chr6_1:78921869-78921888 | None:intergenic | 40.0% |
| ! | TTTAGGACGACCTTTAGGTT+TGG | + | chr6_1:78921876-78921895 | None:intergenic | 40.0% |
| ! | TGTGGATTATGCAAAGGTGA+AGG | - | chr6_1:78921944-78921963 | Msa0893940:CDS | 40.0% |
| ATTGTCACATGTCCGCTAAA+GGG | - | chr6_1:78921974-78921993 | Msa0893940:CDS | 40.0% | |
| TTTGCCTTCTTTCCCTTTAG+CGG | + | chr6_1:78921989-78922008 | None:intergenic | 40.0% | |
| GAAGGTGATAAAGATGGGTC+TGG | - | chr6_1:78919642-78919661 | Msa0893940:CDS | 45.0% | |
| ACAAGAATGGGGTTATGAGC+AGG | - | chr6_1:78919787-78919806 | Msa0893940:CDS | 45.0% | |
| !! | GGATCTTCTGAAGTATTGCG+AGG | - | chr6_1:78920226-78920245 | Msa0893940:intron | 45.0% |
| TTCACGGGGAGAGTTTCTTA+AGG | + | chr6_1:78921139-78921158 | None:intergenic | 45.0% | |
| ! | CTATGAGCTTGCTCTTGTAG+AGG | + | chr6_1:78921458-78921477 | None:intergenic | 45.0% |
| !!! | TGCAGTGTTTTGGGATGGTA+AGG | - | chr6_1:78921607-78921626 | Msa0893940:CDS | 45.0% |
| CGTGATGGCAAGTAGTTAGA+AGG | + | chr6_1:78921725-78921744 | None:intergenic | 45.0% | |
| CTAAAGGTCGTCCTAAACGA+CGG | - | chr6_1:78921879-78921898 | Msa0893940:CDS | 45.0% | |
| CATTGTCACATGTCCGCTAA+AGG | - | chr6_1:78921973-78921992 | Msa0893940:CDS | 45.0% | |
| ATGTCCGCTAAAGGGAAAGA+AGG | - | chr6_1:78921982-78922001 | Msa0893940:CDS | 45.0% | |
| !!! | AATTATTTTTCATTAATTTT+AGG | - | chr6_1:78921343-78921362 | Msa0893940:CDS | 5.0% |
| GTTGTAACTCGCCACTCAGT+TGG | + | chr6_1:78919954-78919973 | None:intergenic | 50.0% | |
| TTTGGTGGGATGACAACCAC+CGG | - | chr6_1:78920980-78920999 | Msa0893940:intron | 50.0% | |
| GATGACAACCACCGGAAGAT+CGG | - | chr6_1:78920988-78921007 | Msa0893940:intron | 50.0% | |
| AATACTCTCCGATCTTCCGG+TGG | + | chr6_1:78920999-78921018 | None:intergenic | 50.0% | |
| CTAACTACTTGCCATCACGG+TGG | - | chr6_1:78921726-78921745 | Msa0893940:CDS | 50.0% | |
| ! | CGTCGTTTAGGACGACCTTT+AGG | + | chr6_1:78921881-78921900 | None:intergenic | 50.0% |
| CCTTTAAGTCGCCGTCGTTT+AGG | + | chr6_1:78921893-78921912 | None:intergenic | 50.0% | |
| CCTAAACGACGGCGACTTAA+AGG | - | chr6_1:78921890-78921909 | Msa0893940:CDS | 50.0% | |
| AAACGACGGCGACTTAAAGG+TGG | - | chr6_1:78921893-78921912 | Msa0893940:CDS | 50.0% | |
| TGGAGGCTCTACCTTCACGA+TGG | + | chr6_1:78919827-78919846 | None:intergenic | 55.0% | |
| GCTTGAAGCAACCACCGTGA+TGG | + | chr6_1:78921740-78921759 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 78919423 | 78922069 | 78919423 | ID=Msa0893940;Name=Msa0893940 |
| chr6_1 | mRNA | 78919423 | 78922069 | 78919423 | ID=Msa0893940-mRNA-1;Parent=Msa0893940;Name=Msa0893940-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|0.2|0.16|6|0|550 |
| chr6_1 | exon | 78921874 | 78922069 | 78921874 | ID=Msa0893940-mRNA-1:exon:13220;Parent=Msa0893940-mRNA-1 |
| chr6_1 | exon | 78921682 | 78921846 | 78921682 | ID=Msa0893940-mRNA-1:exon:13219;Parent=Msa0893940-mRNA-1 |
| chr6_1 | exon | 78921427 | 78921657 | 78921427 | ID=Msa0893940-mRNA-1:exon:13218;Parent=Msa0893940-mRNA-1 |
| chr6_1 | exon | 78921186 | 78921405 | 78921186 | ID=Msa0893940-mRNA-1:exon:13217;Parent=Msa0893940-mRNA-1 |
| chr6_1 | exon | 78920414 | 78920549 | 78920414 | ID=Msa0893940-mRNA-1:exon:13216;Parent=Msa0893940-mRNA-1 |
| chr6_1 | exon | 78919423 | 78920127 | 78919423 | ID=Msa0893940-mRNA-1:exon:13215;Parent=Msa0893940-mRNA-1 |
| chr6_1 | CDS | 78921874 | 78922069 | 78921874 | ID=Msa0893940-mRNA-1:cds;Parent=Msa0893940-mRNA-1 |
| chr6_1 | CDS | 78921682 | 78921846 | 78921682 | ID=Msa0893940-mRNA-1:cds;Parent=Msa0893940-mRNA-1 |
| chr6_1 | CDS | 78921427 | 78921657 | 78921427 | ID=Msa0893940-mRNA-1:cds;Parent=Msa0893940-mRNA-1 |
| chr6_1 | CDS | 78921186 | 78921405 | 78921186 | ID=Msa0893940-mRNA-1:cds;Parent=Msa0893940-mRNA-1 |
| chr6_1 | CDS | 78920414 | 78920549 | 78920414 | ID=Msa0893940-mRNA-1:cds;Parent=Msa0893940-mRNA-1 |
| chr6_1 | CDS | 78919423 | 78920127 | 78919423 | ID=Msa0893940-mRNA-1:cds;Parent=Msa0893940-mRNA-1 |
| Gene Sequence |
| Protein sequence |
