AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0896420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0896420 MtrunA17_Chr6g0481091 94.340 53 3 0 20 72 1 53 4.99e-31 109
Msa0896420 MtrunA17_Chr3g0109821 67.143 70 21 1 20 89 1 68 4.86e-27 103
Msa0896420 MtrunA17_Chr2g0309891 61.429 70 25 1 20 89 1 68 2.52e-24 94.4
Msa0896420 MtrunA17_Chr5g0433781 80.392 51 10 0 20 70 1 51 3.33e-23 92.8
Msa0896420 MtrunA17_Chr4g0054791 70.588 51 15 0 20 70 1 51 3.61e-20 82.8
Msa0896420 MtrunA17_Chr5g0400461 53.623 69 30 1 20 88 1 67 3.88e-20 82.8
Msa0896420 MtrunA17_Chr1g0200561 60.606 66 23 2 25 89 19 82 4.63e-20 82.8
Msa0896420 MtrunA17_Chr5g0405211 54.545 66 27 2 25 89 8 71 2.46e-19 80.9
Msa0896420 MtrunA17_Chr5g0398971 53.846 65 29 1 25 89 7 70 5.88e-19 79.7
Msa0896420 MtrunA17_Chr4g0056541 62.500 48 18 0 25 72 7 54 8.78e-19 79.7
Msa0896420 MtrunA17_Chr4g0018391 64.583 48 17 0 25 72 7 54 1.23e-18 79.0
Msa0896420 MtrunA17_Chr4g0017921 51.562 64 31 0 24 87 7 70 1.25e-18 79.0
Msa0896420 MtrunA17_Chr8g0346001 49.206 63 32 0 25 87 15 77 9.20e-18 77.0
Msa0896420 MtrunA17_Chr2g0309331 50.794 63 31 0 25 87 10 72 1.97e-17 75.5
Msa0896420 MtrunA17_Chr3g0130731 56.923 65 24 2 25 89 7 67 7.37e-17 73.6
Msa0896420 MtrunA17_Chr1g0197351 50.649 77 32 2 23 95 40 114 8.80e-17 74.3
Msa0896420 MtrunA17_Chr8g0372121 58.333 48 20 0 25 72 6 53 3.98e-16 72.0
Msa0896420 MtrunA17_Chr4g0047841 52.174 69 29 1 21 89 11 75 4.39e-15 69.3
Msa0896420 MtrunA17_Chr2g0318101 53.030 66 25 2 25 89 15 75 6.20e-15 68.9
Msa0896420 MtrunA17_Chr6g0453131 58.696 46 19 0 25 70 15 60 6.92e-15 68.9
Msa0896420 MtrunA17_Chr3g0124761 53.846 65 26 1 25 89 7 67 7.96e-15 68.2
Msa0896420 MtrunA17_Chr5g0417791 62.500 48 18 0 25 72 3 50 1.61e-14 67.0
Msa0896420 MtrunA17_Chr7g0252971 45.714 70 32 2 21 89 4 68 1.71e-14 67.4
Msa0896420 MtrunA17_Chr5g0417001 45.902 61 29 1 27 87 16 72 2.90e-14 67.0
Msa0896420 MtrunA17_Chr2g0311651 56.818 44 19 0 27 70 24 67 4.32e-14 66.2
Msa0896420 MtrunA17_Chr1g0201021 58.696 46 19 0 27 72 21 66 4.94e-14 66.6
Msa0896420 MtrunA17_Chr2g0311661 56.818 44 19 0 27 70 23 66 6.37e-14 65.5
Msa0896420 MtrunA17_Chr7g0214071 64.583 48 17 0 23 70 13 60 6.78e-14 65.1
Msa0896420 MtrunA17_Chr2g0318081 53.704 54 25 0 17 70 8 61 9.09e-14 65.5
Msa0896420 MtrunA17_Chr5g0428991 60.417 48 19 0 25 72 25 72 9.12e-14 65.9
Msa0896420 MtrunA17_Chr3g0128151 56.250 48 21 0 25 72 22 69 1.43e-13 65.1
Msa0896420 MtrunA17_Chr1g0147421 51.515 66 27 2 25 89 27 88 1.61e-13 64.7
Msa0896420 MtrunA17_Chr2g0316971 60.870 46 18 0 25 70 23 68 2.09e-13 64.7
Msa0896420 MtrunA17_Chr8g0361731 54.545 44 20 0 27 70 16 59 2.86e-13 64.3
Msa0896420 MtrunA17_Chr4g0076731 59.091 44 18 0 27 70 56 99 2.92e-13 64.3
Msa0896420 MtrunA17_Chr4g0042561 58.333 48 20 0 25 72 9 56 2.94e-13 63.5
Msa0896420 MtrunA17_Chr8g0345711 49.231 65 30 2 25 88 12 74 3.19e-13 62.0
Msa0896420 MtrunA17_Chr1g0183741 43.284 67 34 1 23 89 8 70 3.98e-13 63.2
Msa0896420 MtrunA17_Chr8g0384721 63.043 46 17 0 25 70 7 52 4.30e-13 63.2
Msa0896420 MtrunA17_Chr5g0442771 41.935 62 34 1 27 88 20 79 8.65e-13 62.8
Msa0896420 MtrunA17_Chr7g0261411 44.776 67 33 1 23 89 8 70 9.58e-13 62.4
Msa0896420 MtrunA17_Chr3g0113011 46.429 56 30 0 15 70 9 64 1.08e-12 58.5
Msa0896420 MtrunA17_Chr3g0130101 45.455 66 34 1 27 92 9 72 1.71e-12 62.0
Msa0896420 MtrunA17_Chr4g0060931 44.615 65 32 1 6 70 2 62 2.32e-12 61.6
Msa0896420 MtrunA17_Chr8g0364791 45.161 62 34 0 25 86 6 67 2.41e-12 61.6
Msa0896420 MtrunA17_Chr2g0326361 56.522 46 20 0 25 70 14 59 2.86e-12 61.2
Msa0896420 MtrunA17_Chr6g0484441 46.970 66 31 1 24 89 4 65 2.88e-12 61.2
Msa0896420 MtrunA17_Chr8g0359921 49.254 67 30 1 23 89 12 74 3.59e-12 60.8
Msa0896420 MtrunA17_Chr8g0384201 43.548 62 35 0 25 86 6 67 4.97e-12 60.8
Msa0896420 MtrunA17_Chr4g0050701 45.455 66 32 1 25 90 11 72 7.52e-12 58.9
Msa0896420 MtrunA17_Chr6g0453451 56.522 46 20 0 25 70 20 65 7.58e-12 60.1
Msa0896420 MtrunA17_Chr3g0128111 55.556 45 20 0 28 72 45 89 7.66e-12 59.7
Msa0896420 MtrunA17_Chr7g0263931 50.000 48 24 0 23 70 9 56 9.89e-12 59.7
Msa0896420 MtrunA17_Chr3g0144271 54.000 50 22 1 24 72 22 71 1.31e-11 59.7
Msa0896420 MtrunA17_Chr8g0345691 42.500 80 27 4 19 88 4 74 1.41e-11 59.3
Msa0896420 MtrunA17_Chr7g0253011 52.174 46 22 0 25 70 17 62 1.64e-11 59.3
Msa0896420 MtrunA17_Chr3g0113041 44.643 56 31 0 15 70 9 64 2.03e-11 55.1
Msa0896420 MtrunA17_Chr3g0139331 46.154 65 31 1 25 89 11 71 2.64e-11 58.5
Msa0896420 MtrunA17_Chr3g0091921 56.522 46 20 0 25 70 16 61 3.90e-11 58.2
Msa0896420 MtrunA17_Chr8g0345671 57.447 47 19 1 25 70 11 57 4.04e-11 58.2
Msa0896420 MtrunA17_Chr6g0461661 44.898 49 27 0 22 70 15 63 5.55e-11 57.8
Msa0896420 MtrunA17_Chr1g0194791 55.556 45 19 1 27 70 48 92 7.72e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0896420 AT3G17730.1 92.453 53 4 0 20 72 1 53 1.05e-30 109
Msa0896420 AT5G17260.1 86.275 51 7 0 20 70 1 51 6.03e-28 105
Msa0896420 AT3G03200.1 80.392 51 10 0 20 70 1 51 1.32e-25 99.4
Msa0896420 AT1G54330.1 80.392 51 10 0 20 70 1 51 5.44e-25 95.9
Msa0896420 AT1G65910.1 65.714 70 22 1 20 89 1 68 1.74e-24 96.7
Msa0896420 AT4G36160.2 50.000 78 32 3 17 89 2 77 1.15e-19 82.4
Msa0896420 AT1G32770.1 53.968 63 29 0 25 87 16 78 1.36e-19 82.0
Msa0896420 AT5G66300.1 55.882 68 27 2 23 89 10 75 3.71e-19 80.1
Msa0896420 AT2G18060.3 50.704 71 32 2 20 89 4 72 4.28e-19 80.9
Msa0896420 AT2G18060.1 50.704 71 32 2 20 89 4 72 4.28e-19 80.9
Msa0896420 AT5G46590.2 70.833 48 14 0 25 72 6 53 5.51e-19 79.7
Msa0896420 AT5G46590.1 70.833 48 14 0 25 72 6 53 5.84e-19 79.7
Msa0896420 AT4G36160.1 51.515 66 29 2 25 89 10 73 9.64e-19 79.7
Msa0896420 AT4G36160.3 51.515 66 29 2 25 89 10 73 9.64e-19 79.7
Msa0896420 AT4G10350.1 53.125 64 30 0 24 87 8 71 1.32e-18 79.3
Msa0896420 AT4G17980.1 68.627 51 16 0 20 70 1 51 1.69e-18 78.2
Msa0896420 AT4G17980.2 68.627 51 16 0 20 70 1 51 1.77e-18 78.6
Msa0896420 AT3G61910.1 49.206 63 32 0 25 87 11 73 3.07e-18 78.2
Msa0896420 AT1G12260.2 51.471 68 26 2 25 89 7 70 3.26e-18 78.2
Msa0896420 AT1G12260.1 51.471 68 26 2 25 89 7 70 3.37e-18 78.6
Msa0896420 AT1G33280.1 53.125 64 30 0 24 87 7 70 7.68e-18 76.6
Msa0896420 AT2G46770.1 52.381 63 30 0 25 87 16 78 8.74e-18 77.0
Msa0896420 AT1G71930.2 56.716 67 26 2 24 89 8 72 1.28e-17 76.3
Msa0896420 AT1G71930.1 56.716 67 26 2 24 89 8 72 1.28e-17 76.3
Msa0896420 AT3G10490.1 67.347 49 16 0 23 71 25 73 1.69e-17 75.1
Msa0896420 AT3G10490.2 70.213 47 14 0 25 71 27 73 1.90e-17 76.6
Msa0896420 AT3G10480.3 54.545 66 30 0 23 88 25 90 2.56e-17 76.3
Msa0896420 AT3G10480.1 68.085 47 15 0 25 71 27 73 3.33e-17 75.9
Msa0896420 AT3G10480.2 68.085 47 15 0 25 71 27 73 3.53e-17 75.9
Msa0896420 AT1G62700.1 62.500 48 18 0 25 72 7 54 5.10e-17 75.1
Msa0896420 AT1G62700.2 62.500 48 18 0 25 72 7 54 5.10e-17 75.1
Msa0896420 AT3G10500.1 52.239 67 30 1 25 91 9 73 5.51e-17 75.5
Msa0896420 AT1G79580.3 53.846 65 30 0 23 87 15 79 5.55e-17 75.1
Msa0896420 AT1G79580.5 53.846 65 30 0 23 87 15 79 5.55e-17 75.1
Msa0896420 AT1G79580.4 53.846 65 30 0 23 87 15 79 5.55e-17 75.1
Msa0896420 AT1G79580.2 53.846 65 30 0 23 87 15 79 5.55e-17 75.1
Msa0896420 AT1G79580.1 53.846 65 30 0 23 87 15 79 5.55e-17 75.1
Msa0896420 AT5G04410.1 50.746 67 31 1 25 91 9 73 1.83e-16 73.9
Msa0896420 AT3G04070.1 47.143 70 31 2 21 89 6 70 1.87e-16 73.6
Msa0896420 AT3G04070.2 47.143 70 31 2 21 89 6 70 2.07e-16 73.2
Msa0896420 AT5G04395.1 59.184 49 20 0 23 71 26 74 2.55e-16 70.9
Msa0896420 AT5G62380.2 47.692 65 33 1 25 89 7 70 5.90e-16 72.0
Msa0896420 AT5G62380.1 47.692 65 33 1 25 89 7 70 5.90e-16 72.0
Msa0896420 AT1G33060.2 59.574 47 19 0 24 70 23 69 6.08e-16 72.4
Msa0896420 AT1G33060.1 59.574 47 19 0 24 70 23 69 6.31e-16 72.4
Msa0896420 AT1G32510.1 71.739 46 13 0 25 70 6 51 7.04e-16 71.2
Msa0896420 AT5G64060.1 58.491 53 22 0 20 72 1 53 4.18e-15 69.7
Msa0896420 AT5G24590.2 49.153 59 30 0 14 72 2 60 1.93e-14 68.2
Msa0896420 AT1G01720.1 55.385 65 25 1 25 89 7 67 2.23e-14 67.4
Msa0896420 AT4G35580.3 58.696 46 19 0 25 70 9 54 3.33e-14 67.4
Msa0896420 AT4G35580.2 58.696 46 19 0 25 70 9 54 3.33e-14 67.4
Msa0896420 AT1G61110.1 52.308 65 27 1 25 89 16 76 3.71e-14 67.0
Msa0896420 AT5G39820.1 59.091 44 18 0 27 70 22 65 3.72e-14 67.0
Msa0896420 AT4G35580.1 58.696 46 19 0 25 70 9 54 3.72e-14 67.4
Msa0896420 AT2G02450.2 56.818 44 19 0 27 70 53 96 4.21e-14 67.0
Msa0896420 AT5G09330.5 60.377 53 21 0 20 72 1 53 4.36e-14 67.0
Msa0896420 AT5G09330.2 60.377 53 21 0 20 72 1 53 4.36e-14 67.0
Msa0896420 AT5G09330.4 60.377 53 21 0 20 72 1 53 4.36e-14 67.0
Msa0896420 AT5G09330.3 60.377 53 21 0 20 72 1 53 4.36e-14 67.0
Msa0896420 AT5G09330.1 60.377 53 21 0 20 72 1 53 4.36e-14 67.0
Msa0896420 AT3G49530.1 56.250 48 21 0 25 72 13 60 4.73e-14 67.0
Msa0896420 AT2G27300.1 55.102 49 22 0 24 72 13 61 4.73e-14 66.6
Msa0896420 AT3G49530.2 56.250 48 21 0 25 72 13 60 4.95e-14 66.6
Msa0896420 AT2G02450.1 56.818 44 19 0 27 70 53 96 5.01e-14 66.6
Msa0896420 AT5G63790.2 45.070 71 36 1 3 70 35 105 5.24e-14 66.6
Msa0896420 AT5G63790.1 45.070 71 36 1 3 70 25 95 5.40e-14 66.2
Msa0896420 AT5G22290.1 52.174 46 22 0 27 72 23 68 6.56e-14 66.2
Msa0896420 AT2G43000.1 56.818 44 19 0 27 70 20 63 8.48e-14 65.5
Msa0896420 AT3G44290.2 46.970 66 32 1 27 92 16 78 1.30e-13 64.3
Msa0896420 AT2G24430.2 50.000 66 29 1 24 89 15 76 1.64e-13 65.1
Msa0896420 AT2G24430.1 50.000 66 29 1 24 89 15 76 1.64e-13 65.1
Msa0896420 AT3G44290.1 46.970 66 32 1 27 92 16 78 1.66e-13 65.1
Msa0896420 AT5G53950.1 50.769 65 28 1 25 89 17 77 1.67e-13 65.1
Msa0896420 AT2G33480.2 58.696 46 19 0 25 70 15 60 1.91e-13 64.3
Msa0896420 AT2G33480.1 58.696 46 19 0 25 70 15 60 1.94e-13 64.3
Msa0896420 AT1G26870.1 54.348 46 21 0 25 70 16 61 2.10e-13 65.1
Msa0896420 AT1G69490.1 58.696 46 19 0 25 70 9 54 2.46e-13 64.3
Msa0896420 AT5G08790.1 58.333 48 20 0 23 70 5 52 3.30e-13 63.9
Msa0896420 AT4G27410.3 44.286 70 32 1 17 86 6 68 8.70e-13 63.2
Msa0896420 AT3G18400.1 46.269 67 32 1 24 90 4 66 8.97e-13 63.2
Msa0896420 AT5G13180.1 58.696 46 19 0 25 70 14 59 1.15e-12 62.4
Msa0896420 AT5G61430.1 58.696 46 19 0 25 70 16 61 1.18e-12 62.8
Msa0896420 AT4G27410.2 50.000 54 27 0 17 70 6 59 1.39e-12 62.4
Msa0896420 AT1G76420.1 59.184 49 20 0 24 72 21 69 1.55e-12 62.4
Msa0896420 AT1G77450.1 60.417 48 19 0 25 72 10 57 1.90e-12 61.6
Msa0896420 AT5G18270.2 45.588 68 34 1 23 90 19 83 1.91e-12 62.0
Msa0896420 AT1G34180.4 45.333 75 38 1 27 101 18 89 2.07e-12 62.4
Msa0896420 AT1G34180.2 45.333 75 38 1 27 101 18 89 2.13e-12 62.4
Msa0896420 AT3G15170.1 56.522 46 20 0 25 70 20 65 2.14e-12 62.0
Msa0896420 AT1G34190.1 61.364 44 17 0 27 70 18 61 2.19e-12 62.4
Msa0896420 AT5G18270.1 45.588 68 34 1 23 90 19 83 2.57e-12 61.6
Msa0896420 AT3G15500.1 52.941 51 24 0 20 70 9 59 5.01e-12 60.8
Msa0896420 AT5G64530.2 45.588 68 33 1 23 90 1 64 5.91e-12 58.9
Msa0896420 AT1G52890.1 52.941 51 24 0 20 70 9 59 8.15e-12 60.5
Msa0896420 AT1G34180.1 61.364 44 17 0 27 70 18 61 8.46e-12 60.5
Msa0896420 AT1G34180.3 61.364 44 17 0 27 70 18 61 8.54e-12 60.5
Msa0896420 AT3G15510.1 51.613 62 24 2 29 89 21 77 1.20e-11 60.1
Msa0896420 AT5G07680.2 56.250 48 21 0 23 70 1 48 1.45e-11 59.7
Msa0896420 AT5G07680.1 56.250 48 21 0 23 70 15 62 1.68e-11 59.3
Msa0896420 AT5G64530.1 45.588 68 33 1 23 90 1 64 2.20e-11 57.8
Msa0896420 AT5G39610.1 56.250 48 21 0 23 70 18 65 3.13e-11 58.5
Msa0896420 AT3G29035.1 54.167 48 22 0 23 70 22 69 3.40e-11 58.5
Msa0896420 AT3G12977.1 41.250 80 42 2 12 90 4 79 3.99e-11 58.2
Msa0896420 AT1G52880.1 50.820 61 26 1 29 89 21 77 5.93e-11 57.8
Msa0896420 AT3G04060.1 44.118 68 35 1 23 90 18 82 5.99e-11 58.2

Find 24 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATTACCACCAGGTTTC+AGG 0.179522 6_1:-86522653 Msa0896420:CDS
CATCTCCATTGGCTTGCTTT+TGG 0.263133 6_1:-86522153 Msa0896420:CDS
TTGTCAATTATTATCTAAAG+AGG 0.436603 6_1:-86522608 Msa0896420:CDS
CATTTGTAAAGATCAACTTC+AGG 0.436934 6_1:+86522549 None:intergenic
TTCTTGTCTCGGCTCGGCAT+AGG 0.450111 6_1:-86522127 Msa0896420:CDS
TTGTCTCGGCTCGGCATAGG+AGG 0.456130 6_1:-86522124 Msa0896420:CDS
TTGACAAGTTCTTCCTCAGT+TGG 0.458888 6_1:+86522624 None:intergenic
ATCTTTACAAATGCGAACCT+TGG 0.474234 6_1:-86522539 Msa0896420:CDS
TCTTTACAAATGCGAACCTT+GGG 0.496267 6_1:-86522538 Msa0896420:CDS
AAGAAAATTATGCCACCAGT+TGG 0.514795 6_1:-86522672 Msa0896420:CDS
TGCGAACCTTGGGAATTAGC+AGG 0.524054 6_1:-86522528 Msa0896420:intron
AAACCTGGTGGTAATCCAAC+TGG 0.525177 6_1:+86522657 None:intergenic
CCACCAGTTGGATTACCACC+AGG 0.538135 6_1:-86522660 Msa0896420:CDS
TATCTAAAGAGGAAAATCAA+TGG 0.563389 6_1:-86522597 Msa0896420:CDS
ACATGGCTATGCATCTCCAT+TGG 0.583174 6_1:-86522164 Msa0896420:CDS
ATAGAACCTGCTAATTCCCA+AGG 0.594404 6_1:+86522522 None:intergenic
TGTCTCGGCTCGGCATAGGA+GGG 0.601798 6_1:-86522123 Msa0896420:CDS
GTTGGATGAAACCTGAAACC+TGG 0.630716 6_1:+86522642 None:intergenic
ATCATGAAGCAACAAGACCA+AGG 0.673555 6_1:+86522080 None:intergenic
TGGTTTGAAGACACACCACA+TGG 0.675597 6_1:-86522181 Msa0896420:intron
CCTGGTGGTAATCCAACTGG+TGG 0.676464 6_1:+86522660 None:intergenic
TTTCAGGTTTCATCCAACTG+AGG 0.683691 6_1:-86522637 Msa0896420:CDS
AATGGAGATGCATAGCCATG+TGG 0.780549 6_1:+86522166 None:intergenic
GGATGAAACCTGAAACCTGG+TGG 0.791381 6_1:+86522645 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAATAAAAAATTTCTTTT+CGG + chr6_1:86522516-86522535 None:intergenic 10.0%
!! TTGTCAATTATTATCTAAAG+AGG - chr6_1:86522193-86522212 Msa0896420:intron 20.0%
!!! AGAAAATTGTGACTTTTAAT+TGG - chr6_1:86522553-86522572 Msa0896420:CDS 20.0%
! TATCTAAAGAGGAAAATCAA+TGG - chr6_1:86522204-86522223 Msa0896420:intron 25.0%
! ATTTATCAAATTTGCCCTAA+TGG - chr6_1:86522328-86522347 Msa0896420:intron 25.0%
! TTTATCAAATTTGCCCTAAT+GGG - chr6_1:86522329-86522348 Msa0896420:intron 25.0%
!!! TTGTGACTTTTAATTGGTTT+TGG - chr6_1:86522559-86522578 Msa0896420:CDS 25.0%
CATTTGTAAAGATCAACTTC+AGG + chr6_1:86522255-86522274 None:intergenic 30.0%
CCAAATACTAATTCCCATTA+GGG + chr6_1:86522345-86522364 None:intergenic 30.0%
TCCAAATACTAATTCCCATT+AGG + chr6_1:86522346-86522365 None:intergenic 30.0%
!!! TTTTAATTGGTTTTGGCCTA+TGG - chr6_1:86522566-86522585 Msa0896420:CDS 30.0%
!! CAACATGGATTTGTTTTCTA+TGG - chr6_1:86522600-86522619 Msa0896420:CDS 30.0%
AAGAAAATTATGCCACCAGT+TGG - chr6_1:86522129-86522148 Msa0896420:CDS 35.0%
ATCTTTACAAATGCGAACCT+TGG - chr6_1:86522262-86522281 Msa0896420:intron 35.0%
TCTTTACAAATGCGAACCTT+GGG - chr6_1:86522263-86522282 Msa0896420:intron 35.0%
CCCTAATGGGAATTAGTATT+TGG - chr6_1:86522342-86522361 Msa0896420:intron 35.0%
GAAGTAGAAGAGAACCATTA+GGG + chr6_1:86522386-86522405 None:intergenic 35.0%
! GACATGTGTGATGTTTGTTA+GGG + chr6_1:86522458-86522477 None:intergenic 35.0%
TGACATGTGTGATGTTTGTT+AGG + chr6_1:86522459-86522478 None:intergenic 35.0%
TATCGATCTATCACAACCAT+GGG + chr6_1:86522488-86522507 None:intergenic 35.0%
TTATCGATCTATCACAACCA+TGG + chr6_1:86522489-86522508 None:intergenic 35.0%
TGTTGATGCATTCAAACCAT+AGG + chr6_1:86522585-86522604 None:intergenic 35.0%
ATGGTTTGAATGCATCAACA+TGG - chr6_1:86522585-86522604 Msa0896420:CDS 35.0%
AAAAACCAAAAGCAAGCCAA+TGG + chr6_1:86522656-86522675 None:intergenic 35.0%
!!! GCTTTTGGTTTTTCTTGTCT+CGG - chr6_1:86522663-86522682 Msa0896420:CDS 35.0%
!!! CACTTTTATCTTTGGAACCT+TGG - chr6_1:86522704-86522723 Msa0896420:CDS 35.0%
!! TTTCAGGTTTCATCCAACTG+AGG - chr6_1:86522164-86522183 Msa0896420:CDS 40.0%
TTGACAAGTTCTTCCTCAGT+TGG + chr6_1:86522180-86522199 None:intergenic 40.0%
ATAGAACCTGCTAATTCCCA+AGG + chr6_1:86522282-86522301 None:intergenic 40.0%
CACATTCACATTTGCCCTAA+TGG - chr6_1:86522369-86522388 Msa0896420:intron 40.0%
CGAAGTAGAAGAGAACCATT+AGG + chr6_1:86522387-86522406 None:intergenic 40.0%
!!! GAGGGCATCACTTTTATCTT+TGG - chr6_1:86522696-86522715 Msa0896420:CDS 40.0%
AAACCTGGTGGTAATCCAAC+TGG + chr6_1:86522147-86522166 None:intergenic 45.0%
TTGGATTACCACCAGGTTTC+AGG - chr6_1:86522148-86522167 Msa0896420:CDS 45.0%
GTTGGATGAAACCTGAAACC+TGG + chr6_1:86522162-86522181 None:intergenic 45.0%
ATCTATCACAACCATGGGTG+GGG + chr6_1:86522483-86522502 None:intergenic 45.0%
GATCTATCACAACCATGGGT+GGG + chr6_1:86522484-86522503 None:intergenic 45.0%
TGGTTTGAAGACACACCACA+TGG - chr6_1:86522620-86522639 Msa0896420:CDS 45.0%
AATGGAGATGCATAGCCATG+TGG + chr6_1:86522638-86522657 None:intergenic 45.0%
ACATGGCTATGCATCTCCAT+TGG - chr6_1:86522637-86522656 Msa0896420:CDS 45.0%
!!! CATCTCCATTGGCTTGCTTT+TGG - chr6_1:86522648-86522667 Msa0896420:CDS 45.0%
! TGGTTTTTCTTGTCTCGGCT+CGG - chr6_1:86522668-86522687 Msa0896420:CDS 45.0%
GGATGAAACCTGAAACCTGG+TGG + chr6_1:86522159-86522178 None:intergenic 50.0%
TGCGAACCTTGGGAATTAGC+AGG - chr6_1:86522273-86522292 Msa0896420:intron 50.0%
CGATCTATCACAACCATGGG+TGG + chr6_1:86522485-86522504 None:intergenic 50.0%
CCTGGTGGTAATCCAACTGG+TGG + chr6_1:86522144-86522163 None:intergenic 55.0%
CCACCAGTTGGATTACCACC+AGG - chr6_1:86522141-86522160 Msa0896420:CDS 55.0%
ACACATGTCAACCCCACCCA+TGG - chr6_1:86522469-86522488 Msa0896420:intron 55.0%
!! TTCTTGTCTCGGCTCGGCAT+AGG - chr6_1:86522674-86522693 Msa0896420:CDS 55.0%
!! TTGTCTCGGCTCGGCATAGG+AGG - chr6_1:86522677-86522696 Msa0896420:CDS 60.0%
!! TGTCTCGGCTCGGCATAGGA+GGG - chr6_1:86522678-86522697 Msa0896420:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 86522081 86522742 86522081 ID=Msa0896420;Name=Msa0896420
chr6_1 mRNA 86522081 86522742 86522081 ID=Msa0896420-mRNA-1;Parent=Msa0896420;Name=Msa0896420-mRNA-1;_AED=0.32;_eAED=0.62;_QI=0|0|0|1|1|1|2|0|108
chr6_1 exon 86522529 86522742 86522529 ID=Msa0896420-mRNA-1:exon:14626;Parent=Msa0896420-mRNA-1
chr6_1 exon 86522081 86522193 86522081 ID=Msa0896420-mRNA-1:exon:14625;Parent=Msa0896420-mRNA-1
chr6_1 CDS 86522529 86522742 86522529 ID=Msa0896420-mRNA-1:cds;Parent=Msa0896420-mRNA-1
chr6_1 CDS 86522081 86522193 86522081 ID=Msa0896420-mRNA-1:cds;Parent=Msa0896420-mRNA-1
Gene Sequence

>Msa0896420

ATGGTTGTGATGAGTGAAAACACTTTTGCAAGAACTAGAAAGAATAGAAAGAAAATTATGCCACCAGTTGGATTACCACCAGGTTTCAGGTTTCATCCAACTGAGGAAGAACTTGTCAATTATTATCTAAAGAGGAAAATCAATGGTCAAGAAATTGAACTTGATATAATTCCTGAAGTTGATCTTTACAAATGCGAACCTTGGGAATTAGCAGACACACCACATGGCTATGCATCTCCATTGGCTTGCTTTTGGTTTTTCTTGTCTCGGCTCGGCATAGGAGGGCATCACTTTTATCTTTGGAACCTTGGTCTTGTTGCTTCATGA
Protein sequence

>Msa0896420

MVVMSENTFARTRKNRKKIMPPVGLPPGFRFHPTEEELVNYYLKRKINGQEIELDIIPEVDLYKCEPWELADTPHGYASPLACFWFFLSRLGIGGHHFYLWNLGLVAS*