AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0897550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0897550 MtrunA17_Chr7g0214071 88.776 98 9 2 1 96 87 184 3.91e-57 177
Msa0897550 MtrunA17_Chr7g0243371 86.885 61 8 0 1 61 39 99 6.63e-35 116
Msa0897550 MtrunA17_Chr1g0147421 50.515 97 38 2 1 96 103 190 1.69e-23 92.4
Msa0897550 MtrunA17_Chr7g0261411 54.930 71 31 1 1 71 85 154 1.63e-22 89.0
Msa0897550 MtrunA17_Chr3g0139331 52.500 80 37 1 1 79 86 165 1.99e-22 89.4
Msa0897550 MtrunA17_Chr1g0183741 45.745 94 47 2 1 91 85 177 2.21e-22 88.2
Msa0897550 MtrunA17_Chr5g0405211 45.455 88 45 2 1 87 86 171 2.70e-22 89.0
Msa0897550 MtrunA17_Chr1g0200561 53.571 84 36 3 1 83 97 178 3.06e-22 89.0
Msa0897550 MtrunA17_Chr4g0017921 57.333 75 28 3 1 74 86 157 3.15e-22 89.0
Msa0897550 MtrunA17_Chr8g0385991 42.991 107 49 3 1 99 87 189 3.81e-21 84.0
Msa0897550 MtrunA17_Chr3g0092671 47.059 85 41 2 1 83 83 165 3.92e-21 86.7
Msa0897550 MtrunA17_Chr2g0309331 50.667 75 35 2 1 74 88 161 7.83e-21 85.1
Msa0897550 MtrunA17_Chr2g0309891 48.421 95 41 4 1 89 83 175 7.89e-21 85.1
Msa0897550 MtrunA17_Chr1g0197361 47.059 85 41 2 1 83 86 168 8.44e-21 85.9
Msa0897550 MtrunA17_Chr3g0113001 50.588 85 34 3 1 79 40 122 1.29e-20 84.0
Msa0897550 MtrunA17_Chr2g0312321 43.564 101 43 3 1 97 84 174 1.37e-20 84.0
Msa0897550 MtrunA17_Chr4g0018391 52.000 75 33 2 1 74 85 157 1.63e-20 84.3
Msa0897550 MtrunA17_Chr3g0109821 52.688 93 37 5 1 88 83 173 2.70e-20 84.3
Msa0897550 MtrunA17_Chr4g0060931 50.000 84 35 3 1 79 92 173 4.94e-20 82.8
Msa0897550 MtrunA17_Chr6g0461661 48.718 78 35 1 1 73 93 170 7.48e-20 82.8
Msa0897550 MtrunA17_Chr3g0113031 42.017 119 48 5 1 104 15 127 7.59e-20 81.3
Msa0897550 MtrunA17_Chr5g0398971 48.684 76 36 2 1 74 85 159 1.37e-19 81.6
Msa0897550 MtrunA17_Chr8g0346001 47.674 86 31 2 1 74 93 176 1.59e-19 82.0
Msa0897550 MtrunA17_Chr4g0047841 44.444 99 29 2 1 73 90 188 2.53e-19 80.9
Msa0897550 MtrunA17_Chr2g0316971 47.561 82 38 1 1 77 98 179 2.85e-19 80.9
Msa0897550 MtrunA17_Chr4g0042561 50.000 84 33 3 1 77 84 165 2.87e-19 79.7
Msa0897550 MtrunA17_Chr8g0372121 49.333 75 35 2 1 74 84 156 3.31e-19 80.1
Msa0897550 MtrunA17_Chr4g0056541 52.000 75 33 2 1 74 85 157 4.02e-19 80.5
Msa0897550 MtrunA17_Chr5g0428991 50.649 77 33 2 1 74 102 176 4.41e-19 80.9
Msa0897550 MtrunA17_Chr3g0144271 54.839 62 28 0 1 62 99 160 7.49e-19 80.1
Msa0897550 MtrunA17_Chr3g0131911 47.826 92 37 2 1 81 87 178 8.49e-19 78.2
Msa0897550 MtrunA17_Chr7g0263931 40.566 106 55 3 1 99 86 190 9.16e-19 79.3
Msa0897550 MtrunA17_Chr1g0201021 37.037 108 61 3 1 104 96 200 9.95e-19 80.1
Msa0897550 MtrunA17_Chr8g0384201 40.698 86 46 2 1 83 83 166 1.98e-18 79.0
Msa0897550 MtrunA17_Chr3g0130101 41.905 105 56 3 1 102 84 186 2.03e-18 78.6
Msa0897550 MtrunA17_Chr2g0318081 50.633 79 34 2 4 79 94 170 2.12e-18 78.6
Msa0897550 MtrunA17_Chr2g0318101 45.977 87 33 2 1 73 90 176 2.52e-18 78.2
Msa0897550 MtrunA17_Chr5g0417791 48.148 81 33 2 1 74 78 156 3.96e-18 76.6
Msa0897550 MtrunA17_Chr8g0364791 40.698 86 46 2 1 83 83 166 4.51e-18 77.8
Msa0897550 MtrunA17_Chr3g0091921 47.619 84 37 2 1 79 91 172 4.61e-18 77.0
Msa0897550 MtrunA17_Chr6g0484441 50.000 74 34 2 1 73 80 151 7.66e-18 76.6
Msa0897550 MtrunA17_Chr7g0252971 42.308 104 42 5 1 87 83 185 8.12e-18 76.6
Msa0897550 MtrunA17_Chr3g0128111 48.000 75 34 2 2 73 120 192 8.37e-18 75.5
Msa0897550 MtrunA17_Chr5g0417001 40.217 92 44 1 1 81 89 180 8.63e-18 77.0
Msa0897550 MtrunA17_Chr6g0453451 45.238 84 39 2 1 79 95 176 1.01e-17 76.3
Msa0897550 MtrunA17_Chr8g0361731 38.710 93 45 1 1 81 89 181 1.22e-17 76.6
Msa0897550 MtrunA17_Chr3g0128151 50.000 76 33 2 1 73 99 172 1.42e-17 76.6
Msa0897550 MtrunA17_Chr5g0433781 50.000 76 33 3 1 73 83 156 1.99e-17 76.3
Msa0897550 MtrunA17_Chr2g0326361 45.946 74 33 1 4 70 92 165 2.45e-17 75.1
Msa0897550 MtrunA17_Chr5g0400461 38.000 100 57 1 1 95 83 182 2.61e-17 75.5
Msa0897550 MtrunA17_Chr6g0481091 43.023 86 43 3 1 83 83 165 5.59e-17 73.2
Msa0897550 MtrunA17_Chr7g0217721 43.299 97 35 3 1 79 94 188 7.22e-17 74.3
Msa0897550 MtrunA17_Chr7g0253011 41.284 109 56 4 1 102 92 199 8.24e-17 74.3
Msa0897550 MtrunA17_Chr8g0359921 50.000 82 32 4 4 79 92 170 9.25e-17 73.9
Msa0897550 MtrunA17_Chr3g0124761 50.000 80 31 3 1 74 82 158 1.18e-16 73.2
Msa0897550 MtrunA17_Chr8g0384721 44.231 104 43 4 1 97 82 177 1.18e-16 73.2
Msa0897550 MtrunA17_Chr4g0038751 43.243 74 39 2 1 73 90 161 2.08e-16 72.4
Msa0897550 MtrunA17_Chr7g0217711 58.491 53 20 1 1 53 94 144 4.08e-16 69.7
Msa0897550 MtrunA17_Chr3g0130731 47.500 80 33 2 1 74 82 158 6.27e-16 71.2
Msa0897550 MtrunA17_Chr6g0453131 41.667 84 36 3 2 73 91 173 8.55e-15 68.6
Msa0897550 MtrunA17_Chr8g0345671 47.826 69 33 2 2 70 90 155 1.09e-14 68.2
Msa0897550 MtrunA17_Chr4g0050701 38.043 92 47 2 2 84 79 169 1.20e-14 66.2
Msa0897550 MtrunA17_Chr1g0197351 42.391 92 40 3 1 82 119 207 1.59e-14 67.8
Msa0897550 MtrunA17_Chr5g0442771 38.739 111 52 5 1 98 93 200 2.14e-14 67.0
Msa0897550 MtrunA17_Chr4g0054791 37.895 95 55 2 1 91 83 177 2.61e-14 67.0
Msa0897550 MtrunA17_Chr4g0076731 43.210 81 42 3 1 80 129 206 3.72e-14 67.0
Msa0897550 MtrunA17_Chr2g0311651 36.792 106 47 3 1 90 98 199 4.61e-14 66.2
Msa0897550 MtrunA17_Chr2g0321741 42.857 70 34 2 1 70 68 131 1.07e-13 65.5
Msa0897550 MtrunA17_Chr2g0321631 42.857 77 37 3 2 72 86 161 4.79e-13 62.8
Msa0897550 MtrunA17_Chr2g0311661 43.333 90 34 4 1 74 97 185 5.53e-13 63.2
Msa0897550 MtrunA17_Chr7g0266701 37.805 82 42 3 2 75 86 166 9.60e-13 62.0
Msa0897550 MtrunA17_Chr8g0345691 40.000 70 39 2 1 70 90 156 1.44e-12 62.4
Msa0897550 MtrunA17_Chr8g0345721 51.923 52 24 1 1 52 142 192 3.01e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0897550 AT5G13180.1 64.634 82 27 1 1 80 86 167 3.18e-32 113
Msa0897550 AT2G33480.3 60.494 81 27 2 1 78 30 108 8.78e-29 103
Msa0897550 AT2G33480.2 62.025 79 27 2 1 78 87 163 2.97e-28 103
Msa0897550 AT2G33480.1 60.494 81 27 2 1 78 87 165 6.51e-28 102
Msa0897550 AT2G24430.2 54.321 81 35 1 1 79 91 171 8.64e-24 92.8
Msa0897550 AT2G24430.1 54.321 81 35 1 1 79 91 171 8.64e-24 92.8
Msa0897550 AT1G33280.1 54.054 74 32 1 1 74 86 157 1.25e-23 92.4
Msa0897550 AT4G10350.2 53.247 77 31 2 1 74 40 114 3.79e-23 90.9
Msa0897550 AT4G10350.1 53.247 77 31 2 1 74 87 161 7.82e-23 90.9
Msa0897550 AT4G28530.1 51.282 78 37 1 1 78 101 177 4.63e-22 89.0
Msa0897550 AT5G61430.2 52.381 84 33 3 1 79 28 109 1.92e-21 85.9
Msa0897550 AT2G18060.3 53.571 84 35 4 1 82 87 168 2.53e-21 87.0
Msa0897550 AT2G18060.1 53.571 84 35 4 1 82 87 168 2.53e-21 87.0
Msa0897550 AT5G61430.1 52.381 84 33 3 1 79 91 172 4.11e-21 85.9
Msa0897550 AT5G07680.2 52.381 84 33 3 1 79 78 159 4.47e-21 85.5
Msa0897550 AT5G07680.1 52.381 84 33 3 1 79 92 173 4.70e-21 85.9
Msa0897550 AT4G36160.1 50.588 85 37 3 1 82 88 170 5.63e-21 86.3
Msa0897550 AT4G36160.3 50.588 85 37 3 1 82 88 170 5.63e-21 86.3
Msa0897550 AT4G36160.2 50.588 85 37 3 1 82 92 174 5.78e-21 86.3
Msa0897550 AT2G18060.2 53.086 81 34 4 4 82 2 80 6.25e-21 84.7
Msa0897550 AT5G66300.1 53.247 77 30 3 1 74 90 163 7.91e-21 84.7
Msa0897550 AT3G12977.2 40.708 113 60 4 1 109 40 149 8.16e-21 83.6
Msa0897550 AT1G76420.1 50.000 82 38 2 1 80 97 177 1.30e-20 84.7
Msa0897550 AT5G62380.2 54.667 75 31 2 1 74 85 157 1.58e-20 84.7
Msa0897550 AT5G62380.1 54.667 75 31 2 1 74 85 157 1.58e-20 84.7
Msa0897550 AT1G56010.1 47.619 84 36 2 1 78 28 109 1.74e-20 83.2
Msa0897550 AT1G62700.1 52.000 75 33 2 1 74 85 157 1.77e-20 84.7
Msa0897550 AT1G62700.2 52.000 75 33 2 1 74 85 157 1.77e-20 84.7
Msa0897550 AT5G39610.1 48.810 84 36 2 1 79 95 176 2.25e-20 83.6
Msa0897550 AT1G12260.2 48.750 80 38 2 1 79 85 162 2.33e-20 84.0
Msa0897550 AT3G18400.1 51.852 81 35 3 1 79 80 158 2.59e-20 83.6
Msa0897550 AT1G12260.1 48.750 80 38 2 1 79 85 162 3.32e-20 84.0
Msa0897550 AT3G12977.1 40.708 113 60 4 1 109 93 202 3.39e-20 82.8
Msa0897550 AT1G56010.2 47.619 84 36 2 1 78 95 176 3.87e-20 83.2
Msa0897550 AT3G10500.1 47.674 86 40 2 1 83 86 169 5.55e-20 84.0
Msa0897550 AT1G79580.3 50.667 75 34 2 1 74 95 167 6.00e-20 83.2
Msa0897550 AT1G79580.5 50.667 75 34 2 1 74 95 167 6.00e-20 83.2
Msa0897550 AT1G79580.4 50.667 75 34 2 1 74 95 167 6.00e-20 83.2
Msa0897550 AT1G79580.2 50.667 75 34 2 1 74 95 167 6.00e-20 83.2
Msa0897550 AT1G79580.1 50.667 75 34 2 1 74 95 167 6.00e-20 83.2
Msa0897550 AT5G04410.1 48.837 86 39 2 1 83 86 169 8.50e-20 83.2
Msa0897550 AT3G15170.1 50.000 78 34 2 1 73 95 172 1.08e-19 82.0
Msa0897550 AT4G35580.3 50.000 88 36 3 1 82 86 171 2.59e-19 82.0
Msa0897550 AT4G35580.2 50.000 88 36 3 1 82 86 171 2.75e-19 82.0
Msa0897550 AT4G27410.2 44.211 95 48 2 4 95 92 184 2.77e-19 80.5
Msa0897550 AT1G71930.2 50.000 74 34 2 1 73 87 158 2.89e-19 80.9
Msa0897550 AT1G71930.1 50.000 74 34 2 1 73 87 158 2.89e-19 80.9
Msa0897550 AT4G35580.1 50.000 88 36 3 1 82 86 171 3.07e-19 81.6
Msa0897550 AT4G27410.3 44.211 95 48 2 4 95 109 201 3.09e-19 80.9
Msa0897550 AT3G10480.3 45.882 85 40 2 1 81 137 219 3.59e-19 81.6
Msa0897550 AT5G53950.1 51.282 78 33 2 1 73 92 169 6.23e-19 80.5
Msa0897550 AT1G77450.1 44.000 100 47 3 1 94 85 181 6.26e-19 79.0
Msa0897550 AT5G18270.2 41.228 114 60 3 1 108 96 208 6.50e-19 80.1
Msa0897550 AT5G18270.1 41.228 114 60 3 1 108 96 208 6.70e-19 80.1
Msa0897550 AT1G69490.1 50.000 86 33 3 1 79 84 166 8.22e-19 79.0
Msa0897550 AT3G10480.1 45.882 85 40 2 1 81 104 186 8.59e-19 80.5
Msa0897550 AT1G65910.1 44.330 97 48 4 1 94 83 176 1.26e-18 80.1
Msa0897550 AT3G10490.4 45.882 85 40 2 1 81 4 86 1.39e-18 79.3
Msa0897550 AT3G10490.3 45.882 85 40 2 1 81 4 86 1.39e-18 79.3
Msa0897550 AT3G15510.1 45.977 87 33 2 1 73 92 178 1.39e-18 79.3
Msa0897550 AT3G04060.1 53.846 78 30 3 1 73 95 171 1.60e-18 79.0
Msa0897550 AT3G10490.2 45.882 85 40 2 1 81 104 186 2.08e-18 79.3
Msa0897550 AT3G49530.1 42.000 100 46 3 1 97 90 180 3.26e-18 79.0
Msa0897550 AT3G49530.2 42.000 100 46 3 1 97 90 180 4.01e-18 78.6
Msa0897550 AT3G10490.1 45.882 85 40 2 1 81 104 186 4.58e-18 76.6
Msa0897550 AT2G46770.1 44.444 90 32 2 1 74 94 181 6.63e-18 77.4
Msa0897550 AT1G34190.1 46.591 88 38 4 1 83 93 176 6.74e-18 77.8
Msa0897550 AT1G61110.1 47.727 88 30 3 1 73 91 177 9.50e-18 76.6
Msa0897550 AT3G10480.2 45.238 84 41 2 1 81 104 185 1.05e-17 77.4
Msa0897550 AT5G24590.2 42.308 104 45 4 1 94 90 188 1.22e-17 77.0
Msa0897550 AT1G33060.1 50.000 76 33 2 1 73 101 174 1.27e-17 77.0
Msa0897550 AT1G33060.2 50.000 76 33 2 1 73 101 174 1.29e-17 77.0
Msa0897550 AT1G52890.1 49.367 79 35 2 4 79 92 168 1.68e-17 75.9
Msa0897550 AT3G15500.1 48.148 81 37 2 4 81 92 170 2.43e-17 75.5
Msa0897550 AT3G17730.1 46.053 76 36 3 1 73 83 156 3.15e-17 74.3
Msa0897550 AT5G17260.1 45.455 77 36 2 1 73 83 157 5.53e-17 75.5
Msa0897550 AT1G32770.1 43.678 87 34 3 1 74 94 178 7.61e-17 74.7
Msa0897550 AT5G63790.1 48.780 82 31 3 1 74 125 203 1.07e-16 73.9
Msa0897550 AT5G63790.2 48.780 82 31 3 1 74 135 213 1.17e-16 73.9
Msa0897550 AT5G08790.1 48.780 82 31 3 1 74 82 160 1.18e-16 73.6
Msa0897550 AT5G22290.1 42.553 94 46 3 1 94 97 182 1.26e-16 73.9
Msa0897550 AT3G61910.1 44.944 89 31 3 1 73 89 175 1.66e-16 73.6
Msa0897550 AT1G34180.4 43.678 87 42 3 1 83 105 188 2.22e-16 73.6
Msa0897550 AT1G34180.2 43.678 87 42 3 1 83 105 188 2.31e-16 73.6
Msa0897550 AT2G17040.1 46.575 73 36 2 2 73 82 152 2.46e-16 72.4
Msa0897550 AT5G46590.1 47.368 76 35 2 2 73 84 158 2.46e-16 72.8
Msa0897550 AT1G34180.3 43.678 87 42 3 1 83 93 176 2.80e-16 73.2
Msa0897550 AT1G34180.1 43.678 87 42 3 1 83 93 176 2.91e-16 73.2
Msa0897550 AT5G09330.5 41.860 86 45 2 1 83 83 166 3.07e-16 73.2
Msa0897550 AT5G09330.2 41.860 86 45 2 1 83 83 166 3.07e-16 73.2
Msa0897550 AT5G09330.4 41.860 86 45 2 1 83 83 166 3.07e-16 73.2
Msa0897550 AT5G09330.3 41.860 86 45 2 1 83 83 166 3.07e-16 73.2
Msa0897550 AT5G09330.1 41.860 86 45 2 1 83 83 166 3.07e-16 73.2
Msa0897550 AT1G54330.1 52.055 73 33 1 1 73 83 153 3.07e-16 72.4
Msa0897550 AT3G29035.1 44.211 95 44 3 1 90 99 189 3.46e-16 72.4
Msa0897550 AT1G01720.1 45.455 88 38 3 1 81 82 166 4.25e-16 72.0
Msa0897550 AT5G22290.2 42.222 90 44 3 5 94 2 83 4.49e-16 71.2
Msa0897550 AT3G04070.2 47.674 86 32 3 1 73 85 170 4.50e-16 72.4
Msa0897550 AT3G12910.1 41.379 87 35 3 1 71 97 183 1.04e-15 70.9
Msa0897550 AT3G03200.1 44.156 77 37 2 1 73 83 157 1.38e-15 71.2
Msa0897550 AT2G02450.2 48.684 76 36 3 2 76 127 200 1.58e-15 71.2
Msa0897550 AT4G01550.1 41.667 96 43 4 1 95 82 165 2.18e-15 70.9
Msa0897550 AT1G32510.1 39.423 104 54 2 1 95 83 186 3.08e-15 69.7
Msa0897550 AT2G43000.2 40.426 94 49 4 1 90 15 105 5.98e-15 67.4
Msa0897550 AT2G02450.1 48.052 77 37 3 1 76 126 200 6.24e-15 69.3
Msa0897550 AT4G01540.1 54.545 55 24 1 1 54 83 137 1.56e-14 68.2
Msa0897550 AT4G01540.3 54.545 55 24 1 1 54 83 137 1.79e-14 68.2
Msa0897550 AT2G43000.1 42.857 84 41 4 1 80 93 173 1.83e-14 67.4
Msa0897550 AT4G01540.2 54.545 55 24 1 1 54 83 137 1.83e-14 68.2
Msa0897550 AT4G01540.4 54.545 55 24 1 1 54 83 137 2.19e-14 67.8
Msa0897550 AT3G04070.1 55.738 61 24 1 1 58 85 145 2.48e-14 67.4
Msa0897550 AT2G27300.1 45.714 70 32 2 1 70 90 153 2.97e-14 67.4
Msa0897550 AT4G17980.1 52.830 53 25 0 1 53 83 135 3.46e-14 66.6
Msa0897550 AT3G44290.2 40.659 91 45 3 1 91 90 171 4.51e-14 65.9
Msa0897550 AT4G17980.2 52.830 53 25 0 1 53 83 135 4.51e-14 66.6
Msa0897550 AT5G46590.2 46.032 63 33 1 2 64 84 145 4.61e-14 66.2
Msa0897550 AT1G32870.1 42.045 88 44 3 1 83 85 170 4.94e-14 67.0
Msa0897550 AT1G32870.3 42.045 88 44 3 1 83 121 206 5.12e-14 67.0
Msa0897550 AT1G32870.2 42.045 88 44 3 1 83 85 170 5.23e-14 67.0
Msa0897550 AT4G01520.1 52.727 55 25 1 1 54 83 137 5.27e-14 66.2
Msa0897550 AT1G26870.1 40.000 85 39 2 1 73 91 175 6.04e-14 66.6
Msa0897550 AT1G52880.1 40.659 91 41 3 1 78 92 182 6.24e-14 66.2
Msa0897550 AT3G44290.1 40.659 91 45 3 1 91 90 171 6.73e-14 66.2
Msa0897550 AT5G64060.1 35.185 108 64 3 1 105 83 187 1.15e-13 65.9
Msa0897550 AT5G39820.1 36.082 97 38 2 1 73 95 191 1.62e-13 65.1
Msa0897550 AT3G44350.2 43.421 76 38 2 2 73 86 160 1.45e-12 62.0
Msa0897550 AT5G22380.1 42.105 76 38 2 2 72 87 161 1.92e-12 61.6
Msa0897550 AT3G04420.1 40.278 72 39 2 2 73 83 150 2.20e-11 59.3
Msa0897550 AT3G04420.2 40.278 72 39 2 2 73 83 150 2.24e-11 58.9
Msa0897550 AT3G44350.1 49.123 57 28 1 2 58 86 141 3.09e-11 57.0
Msa0897550 AT1G02230.1 39.394 99 47 5 2 99 83 169 3.31e-11 58.9
Msa0897550 AT3G55210.1 38.750 80 44 3 2 80 101 176 4.86e-11 58.2
Msa0897550 AT5G04395.1 49.091 55 27 1 1 54 116 170 8.45e-11 56.6

Find 25 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTACAAACTCTGGTTAC+TGG 0.345872 6_1:+89911800 None:intergenic
CCAAGCAGTGGCAGCAACTT+TGG 0.381811 6_1:+89912158 Msa0897550:CDS
TTACTGGAAGGCAACTGGTT+TGG 0.413426 6_1:+89911816 Msa0897550:CDS
CCAAAGTTGCTGCCACTGCT+TGG 0.433802 6_1:-89912158 None:intergenic
CTTGATTACACCCTTGATCA+TGG 0.437127 6_1:+89912627 Msa0897550:CDS
TCTGGTTACTGGAAGGCAAC+TGG 0.438388 6_1:+89911811 Msa0897550:CDS
TCGAAGACTCGTGAAGTTGT+TGG 0.445139 6_1:+89911856 Msa0897550:CDS
TGCAGAATGAAAATTGGGTT+CGG 0.450439 6_1:+89912069 Msa0897550:intron
TTCCTCTCACTCCAACCCCT+TGG 0.484311 6_1:+89911963 Msa0897550:CDS
AAACGAGACAACCATGATCA+AGG 0.489124 6_1:-89912638 None:intergenic
GGTTGGAGTGAGAGGAAGTG+AGG 0.511570 6_1:-89911957 None:intergenic
AATGGAGAAGAGACAGTGAA+AGG 0.546634 6_1:+89912128 Msa0897550:CDS
GACCAAGGGGTTGGAGTGAG+AGG 0.548262 6_1:-89911965 None:intergenic
CTCATGGTTCAAGAACTGAT+TGG 0.548704 6_1:+89911917 Msa0897550:CDS
AGAAAGACTGACCAAGGGGT+TGG 0.548887 6_1:-89911974 None:intergenic
TCTAACAGAGCTACAAACTC+TGG 0.576816 6_1:+89911793 None:intergenic
AACGAGACAACCATGATCAA+GGG 0.603035 6_1:-89912637 None:intergenic
AAAGGAGTGAAGCCAAGCAG+TGG 0.603258 6_1:+89912146 Msa0897550:CDS
TACATAGGGAAACCACCTCA+TGG 0.606934 6_1:+89911901 Msa0897550:CDS
CGCATATTTCTGAAGAGGAG+AGG 0.611502 6_1:+89912095 Msa0897550:CDS
TCAGTTCTTGAACCATGAGG+TGG 0.615010 6_1:-89911913 None:intergenic
GGTGTCGCATATTTCTGAAG+AGG 0.616757 6_1:+89912090 Msa0897550:CDS
TACAAACTCTGGTTACTGGA+AGG 0.635081 6_1:+89911804 Msa0897550:CDS
ATATTTCTGAAGAGGAGAGG+TGG 0.674268 6_1:+89912098 Msa0897550:CDS
CAATCAGTTCTTGAACCATG+AGG 0.707588 6_1:-89911916 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATTAATATGAATGAAT+TGG + chr6_1:89912042-89912061 Msa0897550:intron 10.0%
!! TAATTTATGTTATAAAACTA+AGG - chr6_1:89912496-89912515 None:intergenic 10.0%
!! TAATATTAATATGAATGAAT+TGG + chr6_1:89912042-89912061 Msa0897550:intron 10.0%
!! TAATTTATGTTATAAAACTA+AGG - chr6_1:89912496-89912515 None:intergenic 10.0%
!! ATTGTTATGAATATTATAAG+TGG + chr6_1:89912248-89912267 Msa0897550:intron 15.0%
!! ATTGTTATGAATATTATAAG+TGG + chr6_1:89912248-89912267 Msa0897550:intron 15.0%
!!! AAAACTATTGTTTTCTACAT+AGG + chr6_1:89911886-89911905 Msa0897550:CDS 20.0%
!!! AAACTATTGTTTTCTACATA+GGG + chr6_1:89911887-89911906 Msa0897550:CDS 20.0%
!! TTACAAATTTAGACAAAGAA+AGG - chr6_1:89912005-89912024 None:intergenic 20.0%
!! TGTTATAAAACTAAGGAAAT+AGG - chr6_1:89912489-89912508 None:intergenic 20.0%
!! TTTGTACTTTCATATCTTAT+AGG - chr6_1:89912525-89912544 None:intergenic 20.0%
!!! AAAACTATTGTTTTCTACAT+AGG + chr6_1:89911886-89911905 Msa0897550:CDS 20.0%
!!! AAACTATTGTTTTCTACATA+GGG + chr6_1:89911887-89911906 Msa0897550:CDS 20.0%
!! TTACAAATTTAGACAAAGAA+AGG - chr6_1:89912005-89912024 None:intergenic 20.0%
!! TGTTATAAAACTAAGGAAAT+AGG - chr6_1:89912489-89912508 None:intergenic 20.0%
!! TTTGTACTTTCATATCTTAT+AGG - chr6_1:89912525-89912544 None:intergenic 20.0%
!! GATTTTGCAGAATGAAAATT+GGG + chr6_1:89912064-89912083 Msa0897550:intron 25.0%
!! GATTTTGCAGAATGAAAATT+GGG + chr6_1:89912064-89912083 Msa0897550:intron 25.0%
! GGATTTTGCAGAATGAAAAT+TGG + chr6_1:89912063-89912082 Msa0897550:intron 30.0%
TGGATGTACATACAACAATT+GGG + chr6_1:89912268-89912287 Msa0897550:intron 30.0%
ACATACAACAATTGGGTTAT+TGG + chr6_1:89912275-89912294 Msa0897550:intron 30.0%
CATACAACAATTGGGTTATT+GGG + chr6_1:89912276-89912295 Msa0897550:intron 30.0%
TAGAGAAATACAATGACTTG+AGG - chr6_1:89912360-89912379 None:intergenic 30.0%
TTCTCTTGTAGTGTACAATT+TGG - chr6_1:89912403-89912422 None:intergenic 30.0%
! GGATTTTGCAGAATGAAAAT+TGG + chr6_1:89912063-89912082 Msa0897550:intron 30.0%
TGGATGTACATACAACAATT+GGG + chr6_1:89912268-89912287 Msa0897550:intron 30.0%
ACATACAACAATTGGGTTAT+TGG + chr6_1:89912275-89912294 Msa0897550:intron 30.0%
CATACAACAATTGGGTTATT+GGG + chr6_1:89912276-89912295 Msa0897550:intron 30.0%
TAGAGAAATACAATGACTTG+AGG - chr6_1:89912360-89912379 None:intergenic 30.0%
TTCTCTTGTAGTGTACAATT+TGG - chr6_1:89912403-89912422 None:intergenic 30.0%
AAGAAAGAAAGACTGACCAA+GGG - chr6_1:89911982-89912001 None:intergenic 35.0%
TGCAGAATGAAAATTGGGTT+CGG + chr6_1:89912069-89912088 Msa0897550:intron 35.0%
GTGGATGTACATACAACAAT+TGG + chr6_1:89912267-89912286 Msa0897550:intron 35.0%
! GGGTCATACTATTAGCTTAT+TGG + chr6_1:89912296-89912315 Msa0897550:intron 35.0%
AAAGTAGAGAGAAGTAGAGA+AGG - chr6_1:89912457-89912476 None:intergenic 35.0%
AAGAAAGAAAGACTGACCAA+GGG - chr6_1:89911982-89912001 None:intergenic 35.0%
TGCAGAATGAAAATTGGGTT+CGG + chr6_1:89912069-89912088 Msa0897550:intron 35.0%
GTGGATGTACATACAACAAT+TGG + chr6_1:89912267-89912286 Msa0897550:intron 35.0%
! GGGTCATACTATTAGCTTAT+TGG + chr6_1:89912296-89912315 Msa0897550:intron 35.0%
AAAGTAGAGAGAAGTAGAGA+AGG - chr6_1:89912457-89912476 None:intergenic 35.0%
TACAAACTCTGGTTACTGGA+AGG + chr6_1:89911804-89911823 Msa0897550:CDS 40.0%
CAATCAGTTCTTGAACCATG+AGG - chr6_1:89911919-89911938 None:intergenic 40.0%
CTCATGGTTCAAGAACTGAT+TGG + chr6_1:89911917-89911936 Msa0897550:CDS 40.0%
AGAAAGAAAGACTGACCAAG+GGG - chr6_1:89911981-89912000 None:intergenic 40.0%
GAAGAAAGAAAGACTGACCA+AGG - chr6_1:89911983-89912002 None:intergenic 40.0%
! ATATTTCTGAAGAGGAGAGG+TGG + chr6_1:89912098-89912117 Msa0897550:CDS 40.0%
AATGGAGAAGAGACAGTGAA+AGG + chr6_1:89912128-89912147 Msa0897550:CDS 40.0%
! CTTGGAGAGAAAGCACAATA+TGG - chr6_1:89912322-89912341 None:intergenic 40.0%
GTGTAATCAAGCAGTTCTGT+CGG - chr6_1:89912618-89912637 None:intergenic 40.0%
CTTGATTACACCCTTGATCA+TGG + chr6_1:89912627-89912646 Msa0897550:CDS 40.0%
AACGAGACAACCATGATCAA+GGG - chr6_1:89912640-89912659 None:intergenic 40.0%
AAACGAGACAACCATGATCA+AGG - chr6_1:89912641-89912660 None:intergenic 40.0%
TACAAACTCTGGTTACTGGA+AGG + chr6_1:89911804-89911823 Msa0897550:CDS 40.0%
CAATCAGTTCTTGAACCATG+AGG - chr6_1:89911919-89911938 None:intergenic 40.0%
CTCATGGTTCAAGAACTGAT+TGG + chr6_1:89911917-89911936 Msa0897550:CDS 40.0%
AGAAAGAAAGACTGACCAAG+GGG - chr6_1:89911981-89912000 None:intergenic 40.0%
GAAGAAAGAAAGACTGACCA+AGG - chr6_1:89911983-89912002 None:intergenic 40.0%
! ATATTTCTGAAGAGGAGAGG+TGG + chr6_1:89912098-89912117 Msa0897550:CDS 40.0%
AATGGAGAAGAGACAGTGAA+AGG + chr6_1:89912128-89912147 Msa0897550:CDS 40.0%
! CTTGGAGAGAAAGCACAATA+TGG - chr6_1:89912322-89912341 None:intergenic 40.0%
GTGTAATCAAGCAGTTCTGT+CGG - chr6_1:89912618-89912637 None:intergenic 40.0%
CTTGATTACACCCTTGATCA+TGG + chr6_1:89912627-89912646 Msa0897550:CDS 40.0%
AACGAGACAACCATGATCAA+GGG - chr6_1:89912640-89912659 None:intergenic 40.0%
AAACGAGACAACCATGATCA+AGG - chr6_1:89912641-89912660 None:intergenic 40.0%
TTACTGGAAGGCAACTGGTT+TGG + chr6_1:89911816-89911835 Msa0897550:CDS 45.0%
TCGAAGACTCGTGAAGTTGT+TGG + chr6_1:89911856-89911875 Msa0897550:CDS 45.0%
TACATAGGGAAACCACCTCA+TGG + chr6_1:89911901-89911920 Msa0897550:CDS 45.0%
TCAGTTCTTGAACCATGAGG+TGG - chr6_1:89911916-89911935 None:intergenic 45.0%
! GGTGTCGCATATTTCTGAAG+AGG + chr6_1:89912090-89912109 Msa0897550:CDS 45.0%
! CGCATATTTCTGAAGAGGAG+AGG + chr6_1:89912095-89912114 Msa0897550:CDS 45.0%
! AGGAGAGGTGGTGCTAAAAA+TGG + chr6_1:89912110-89912129 Msa0897550:CDS 45.0%
CTCAGCTTCAAGTGCAAAAG+TGG + chr6_1:89912199-89912218 Msa0897550:intron 45.0%
GTGCTTTCTCTCCAAGTCAT+TGG + chr6_1:89912326-89912345 Msa0897550:intron 45.0%
AGGAACAAGCACCAATGACT+TGG - chr6_1:89912340-89912359 None:intergenic 45.0%
TTACTGGAAGGCAACTGGTT+TGG + chr6_1:89911816-89911835 Msa0897550:CDS 45.0%
TCGAAGACTCGTGAAGTTGT+TGG + chr6_1:89911856-89911875 Msa0897550:CDS 45.0%
TACATAGGGAAACCACCTCA+TGG + chr6_1:89911901-89911920 Msa0897550:CDS 45.0%
TCAGTTCTTGAACCATGAGG+TGG - chr6_1:89911916-89911935 None:intergenic 45.0%
! GGTGTCGCATATTTCTGAAG+AGG + chr6_1:89912090-89912109 Msa0897550:CDS 45.0%
! CGCATATTTCTGAAGAGGAG+AGG + chr6_1:89912095-89912114 Msa0897550:CDS 45.0%
! AGGAGAGGTGGTGCTAAAAA+TGG + chr6_1:89912110-89912129 Msa0897550:CDS 45.0%
CTCAGCTTCAAGTGCAAAAG+TGG + chr6_1:89912199-89912218 Msa0897550:intron 45.0%
GTGCTTTCTCTCCAAGTCAT+TGG + chr6_1:89912326-89912345 Msa0897550:intron 45.0%
AGGAACAAGCACCAATGACT+TGG - chr6_1:89912340-89912359 None:intergenic 45.0%
!!! TTTATTTTGAATAAAATTAT+AGG - chr6_1:89912557-89912576 None:intergenic 5.0%
!!! TTTATTTTGAATAAAATTAT+AGG - chr6_1:89912557-89912576 None:intergenic 5.0%
TCTGGTTACTGGAAGGCAAC+TGG + chr6_1:89911811-89911830 Msa0897550:CDS 50.0%
AGAAAGACTGACCAAGGGGT+TGG - chr6_1:89911977-89911996 None:intergenic 50.0%
AAAGGAGTGAAGCCAAGCAG+TGG + chr6_1:89912146-89912165 Msa0897550:CDS 50.0%
TCTGGTTACTGGAAGGCAAC+TGG + chr6_1:89911811-89911830 Msa0897550:CDS 50.0%
AGAAAGACTGACCAAGGGGT+TGG - chr6_1:89911977-89911996 None:intergenic 50.0%
AAAGGAGTGAAGCCAAGCAG+TGG + chr6_1:89912146-89912165 Msa0897550:CDS 50.0%
GGTTGGAGTGAGAGGAAGTG+AGG - chr6_1:89911960-89911979 None:intergenic 55.0%
TTCCTCTCACTCCAACCCCT+TGG + chr6_1:89911963-89911982 Msa0897550:CDS 55.0%
CCAAAGTTGCTGCCACTGCT+TGG - chr6_1:89912161-89912180 None:intergenic 55.0%
CCAAGCAGTGGCAGCAACTT+TGG + chr6_1:89912158-89912177 Msa0897550:CDS 55.0%
AGCAGTGGCAGCAACTTTGG+TGG + chr6_1:89912161-89912180 Msa0897550:intron 55.0%
GCAGTGGCAGCAACTTTGGT+GGG + chr6_1:89912162-89912181 Msa0897550:intron 55.0%
CAGTGGCAGCAACTTTGGTG+GGG + chr6_1:89912163-89912182 Msa0897550:intron 55.0%
GGTTGGAGTGAGAGGAAGTG+AGG - chr6_1:89911960-89911979 None:intergenic 55.0%
TTCCTCTCACTCCAACCCCT+TGG + chr6_1:89911963-89911982 Msa0897550:CDS 55.0%
CCAAAGTTGCTGCCACTGCT+TGG - chr6_1:89912161-89912180 None:intergenic 55.0%
CCAAGCAGTGGCAGCAACTT+TGG + chr6_1:89912158-89912177 Msa0897550:CDS 55.0%
AGCAGTGGCAGCAACTTTGG+TGG + chr6_1:89912161-89912180 Msa0897550:intron 55.0%
GCAGTGGCAGCAACTTTGGT+GGG + chr6_1:89912162-89912181 Msa0897550:intron 55.0%
CAGTGGCAGCAACTTTGGTG+GGG + chr6_1:89912163-89912182 Msa0897550:intron 55.0%
GACCAAGGGGTTGGAGTGAG+AGG - chr6_1:89911968-89911987 None:intergenic 60.0%
GACCAAGGGGTTGGAGTGAG+AGG - chr6_1:89911968-89911987 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 89911802 89912662 89911802 ID=Msa0897550;Name=Msa0897550
chr6_1 mRNA 89911802 89912662 89911802 ID=Msa0897550-mRNA-1;Parent=Msa0897550;Name=Msa0897550-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0|0.66|1|1|3|0|109
chr6_1 exon 89911802 89911984 89911802 ID=Msa0897550-mRNA-1:exon:15263;Parent=Msa0897550-mRNA-1
chr6_1 exon 89912074 89912179 89912074 ID=Msa0897550-mRNA-1:exon:15264;Parent=Msa0897550-mRNA-1
chr6_1 exon 89912622 89912662 89912622 ID=Msa0897550-mRNA-1:exon:15265;Parent=Msa0897550-mRNA-1
chr6_1 CDS 89911802 89911984 89911802 ID=Msa0897550-mRNA-1:cds;Parent=Msa0897550-mRNA-1
chr6_1 CDS 89912074 89912179 89912074 ID=Msa0897550-mRNA-1:cds;Parent=Msa0897550-mRNA-1
chr6_1 CDS 89912622 89912662 89912622 ID=Msa0897550-mRNA-1:cds;Parent=Msa0897550-mRNA-1
Gene Sequence

>Msa0897550

GCTACAAACTCTGGTTACTGGAAGGCAACTGGTTTGGACAAACAAATCATGAATTCGAAGACTCGTGAAGTTGTTGGTATGAAGAAAACTATTGTTTTCTACATAGGGAAACCACCTCATGGTTCAAGAACTGATTGGATCATGCATGAATATCACCTCACTTCCTCTCACTCCAACCCCTTGAATGAAAATTGGGTTCGGTGTCGCATATTTCTGAAGAGGAGAGGTGGTGCTAAAAATGGAGAAGAGACAGTGAAAGGAGTGAAGCCAAGCAGTGGCAGCAACTTTGAACTGCTTGATTACACCCTTGATCATGGTTGTCTCGTTTAA
Protein sequence

>Msa0897550

ATNSGYWKATGLDKQIMNSKTREVVGMKKTIVFYIGKPPHGSRTDWIMHEYHLTSSHSNPLNENWVRCRIFLKRRGGAKNGEETVKGVKPSSGSNFELLDYTLDHGCLV*