Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898520 | QSD99670.1 | 89.744 | 39 | 4 | 0 | 1 | 39 | 1 | 39 | 7.10e-16 | 79.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898520 | A0A896WC68 | 89.744 | 39 | 4 | 0 | 1 | 39 | 1 | 39 | 3.39e-16 | 79.0 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0898520 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898520 | MtrunA17_Chr1g0209661 | 72.222 | 36 | 10 | 0 | 4 | 39 | 21 | 56 | 1.32e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATGATAATGAACTTAGA+AGG | 0.345561 | 6_1:-92717031 | Msa0898520:CDS |
| TCTTGGCTAGCTATGAGTTC+AGG | 0.377605 | 6_1:-92716935 | Msa0898520:intron |
| TAAAGGGAGATGGAATTTCT+TGG | 0.407300 | 6_1:-92716952 | Msa0898520:CDS |
| CACATTGCTAATCATGGTAA+AGG | 0.424041 | 6_1:-92716969 | Msa0898520:CDS |
| ATCAAACACATTGCTAATCA+TGG | 0.471602 | 6_1:-92716975 | Msa0898520:CDS |
| ACATTGCTAATCATGGTAAA+GGG | 0.503089 | 6_1:-92716968 | Msa0898520:CDS |
| CTAATCATGGTAAAGGGAGA+TGG | 0.516947 | 6_1:-92716962 | Msa0898520:CDS |
| ATGAACTTAGAAGGGAACCT+TGG | 0.543730 | 6_1:-92717022 | Msa0898520:CDS |
| TCATCCTCTTCAAGAGTCCA+AGG | 0.584097 | 6_1:+92717005 | None:intergenic |
| AGATGATAATGAACTTAGAA+GGG | 0.590350 | 6_1:-92717030 | Msa0898520:CDS |
| GGAACCTTGGACTCTTGAAG+AGG | 0.638793 | 6_1:-92717009 | Msa0898520:CDS |
| GATTAGCAATGTGTTTGATG+AGG | 0.678454 | 6_1:+92716979 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATTTATCTTTTTTGATTT+AGG | - | chr6_1:92717017-92717036 | Msa0898520:CDS | 15.0% |
| !!! | TCTTTTTTGATTTAGGATTA+AGG | - | chr6_1:92717024-92717043 | Msa0898520:CDS | 20.0% |
| !! | AAGATGATAATGAACTTAGA+AGG | - | chr6_1:92716824-92716843 | Msa0898520:intron | 25.0% |
| !! | AGATGATAATGAACTTAGAA+GGG | - | chr6_1:92716825-92716844 | Msa0898520:intron | 25.0% |
| !!! | TTTTTGATTTAGGATTAAGG+AGG | - | chr6_1:92717027-92717046 | Msa0898520:CDS | 25.0% |
| ATCAAACACATTGCTAATCA+TGG | - | chr6_1:92716880-92716899 | Msa0898520:intron | 30.0% | |
| ACATTGCTAATCATGGTAAA+GGG | - | chr6_1:92716887-92716906 | Msa0898520:intron | 30.0% | |
| !! | AAATTTGCTAGTGCAAGATA+TGG | - | chr6_1:92716948-92716967 | Msa0898520:CDS | 30.0% |
| GATTAGCAATGTGTTTGATG+AGG | + | chr6_1:92716879-92716898 | None:intergenic | 35.0% | |
| CACATTGCTAATCATGGTAA+AGG | - | chr6_1:92716886-92716905 | Msa0898520:intron | 35.0% | |
| TAAAGGGAGATGGAATTTCT+TGG | - | chr6_1:92716903-92716922 | Msa0898520:intron | 35.0% | |
| ATGAACTTAGAAGGGAACCT+TGG | - | chr6_1:92716833-92716852 | Msa0898520:intron | 40.0% | |
| CTAATCATGGTAAAGGGAGA+TGG | - | chr6_1:92716893-92716912 | Msa0898520:intron | 40.0% | |
| TCATCCTCTTCAAGAGTCCA+AGG | + | chr6_1:92716853-92716872 | None:intergenic | 45.0% | |
| ! | TCTTGGCTAGCTATGAGTTC+AGG | - | chr6_1:92716920-92716939 | Msa0898520:intron | 45.0% |
| GGAACCTTGGACTCTTGAAG+AGG | - | chr6_1:92716846-92716865 | Msa0898520:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 92716811 | 92717066 | 92716811 | ID=Msa0898520;Name=Msa0898520 |
| chr6_1 | mRNA | 92716811 | 92717066 | 92716811 | ID=Msa0898520-mRNA-1;Parent=Msa0898520;Name=Msa0898520-mRNA-1;_AED=0.35;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|52 |
| chr6_1 | exon | 92716936 | 92717066 | 92716936 | ID=Msa0898520-mRNA-1:exon:15662;Parent=Msa0898520-mRNA-1 |
| chr6_1 | exon | 92716811 | 92716838 | 92716811 | ID=Msa0898520-mRNA-1:exon:15661;Parent=Msa0898520-mRNA-1 |
| chr6_1 | CDS | 92716936 | 92717066 | 92716936 | ID=Msa0898520-mRNA-1:cds;Parent=Msa0898520-mRNA-1 |
| chr6_1 | CDS | 92716811 | 92716838 | 92716811 | ID=Msa0898520-mRNA-1:cds;Parent=Msa0898520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
