Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901540 | XP_003620925.1 | 92.308 | 52 | 4 | 0 | 1 | 52 | 317 | 368 | 7.08e-25 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901540 | G7KJT8 | 92.308 | 52 | 4 | 0 | 1 | 52 | 317 | 368 | 3.38e-25 | 104 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0901540 | TF | HRT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901540 | MtrunA17_Chr6g0488451 | 92.308 | 52 | 4 | 0 | 1 | 52 | 317 | 368 | 6.51e-29 | 104 |
| Msa0901540 | MtrunA17_Chr7g0256351 | 80.952 | 42 | 8 | 0 | 6 | 47 | 337 | 378 | 1.70e-18 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0901540 | AT4G26170.1 | 54.167 | 48 | 21 | 1 | 3 | 50 | 294 | 340 | 2.49e-11 | 56.6 |
Find 7 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTACATGTGGAATTATTT+TGG | 0.157647 | 6_1:-98283975 | Msa0901540:CDS |
| ATCAATTATTTCTAATTAAT+AGG | 0.256718 | 6_1:+98283866 | None:intergenic |
| TGTAGAAAACAACCAGTTAA+AGG | 0.282189 | 6_1:-98283938 | Msa0901540:CDS |
| AATTCCACATGTAATAGTTT+TGG | 0.293273 | 6_1:+98283982 | None:intergenic |
| AAAAGATGTCATGATCATAA+AGG | 0.370970 | 6_1:-98283911 | Msa0901540:CDS |
| AAAGATGTCATGATCATAAA+GGG | 0.444185 | 6_1:-98283910 | Msa0901540:CDS |
| ATCACCAAAACTATTACATG+TGG | 0.607083 | 6_1:-98283986 | Msa0901540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTACATGTGGAATTATTT+TGG | - | chr6_1:98283915-98283934 | Msa0901540:CDS | 20.0% |
| !! | AATTCCACATGTAATAGTTT+TGG | + | chr6_1:98283911-98283930 | None:intergenic | 25.0% |
| ! | AAAAGATGTCATGATCATAA+AGG | - | chr6_1:98283979-98283998 | Msa0901540:CDS | 25.0% |
| ! | AAAGATGTCATGATCATAAA+GGG | - | chr6_1:98283980-98283999 | Msa0901540:CDS | 25.0% |
| ATCACCAAAACTATTACATG+TGG | - | chr6_1:98283904-98283923 | Msa0901540:CDS | 30.0% | |
| !!! | TGTGGAATTATTTTGGAAGA+TGG | - | chr6_1:98283922-98283941 | Msa0901540:CDS | 30.0% |
| TGTAGAAAACAACCAGTTAA+AGG | - | chr6_1:98283952-98283971 | Msa0901540:CDS | 30.0% | |
| ! | CATCTTTTTCTTCCTTTAAC+TGG | + | chr6_1:98283967-98283986 | None:intergenic | 30.0% |
| !! | AACTGGTTGTTTTCTACAAG+TGG | + | chr6_1:98283950-98283969 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 98283877 | 98284035 | 98283877 | ID=Msa0901540;Name=Msa0901540 |
| chr6_1 | mRNA | 98283877 | 98284035 | 98283877 | ID=Msa0901540-mRNA-1;Parent=Msa0901540;Name=Msa0901540-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|52 |
| chr6_1 | exon | 98283877 | 98284035 | 98283877 | ID=Msa0901540-mRNA-1:exon:17346;Parent=Msa0901540-mRNA-1 |
| chr6_1 | CDS | 98283877 | 98284035 | 98283877 | ID=Msa0901540-mRNA-1:cds;Parent=Msa0901540-mRNA-1 |
| Gene Sequence |
| Protein sequence |
