AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0901910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0901910 MtrunA17_Chr6g0473731 61.714 350 78 6 54 350 6 352 1.05e-131 383
Msa0901910 MtrunA17_Chr7g0223971 60.215 93 37 0 129 221 124 216 9.55e-27 110
Msa0901910 MtrunA17_Chr8g0376881 25.954 262 129 6 6 202 27 288 7.86e-15 75.5
Msa0901910 MtrunA17_Chr7g0223991 34.184 196 75 8 194 338 24 216 2.14e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0901910 AT4G31920.1 27.823 248 121 7 8 203 17 258 1.28e-12 69.3

Find 60 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATATAGCTTTGGGTTAA+TGG 0.137829 6_1:-99009577 Msa0901910:CDS
TCGTTCAATCATTTCAATTA+TGG 0.195385 6_1:-99009693 Msa0901910:CDS
TTAACAAGGAACTTAAAATT+TGG 0.202387 6_1:-99009756 Msa0901910:CDS
ATTTCATTGATGACAGAAAT+TGG 0.248135 6_1:+99009987 None:intergenic
CAGATCCAACATGCAAGTTT+TGG 0.289471 6_1:-99013416 Msa0901910:CDS
AAATGTATCCAGTCATTTAC+AGG 0.301898 6_1:-99010621 Msa0901910:intron
GTAGAAGCGACATCATAAAA+TGG 0.305371 6_1:+99009843 None:intergenic
TCCATTAACCCAAAGCTATA+TGG 0.315314 6_1:+99009576 None:intergenic
GACCAAGATCAAGGGGAGTT+TGG 0.325620 6_1:-99010969 Msa0901910:CDS
ATGTTATTCCATATAGCTTT+GGG 0.337073 6_1:-99009584 Msa0901910:CDS
GTAAGAAACCAAGGCTTGTT+TGG 0.352874 6_1:-99010942 Msa0901910:CDS
TCAATTATGGTGTGAAGAAC+TGG 0.359272 6_1:-99009680 Msa0901910:CDS
AATGTTATTCCATATAGCTT+TGG 0.374067 6_1:-99009585 Msa0901910:CDS
GGACCATGATGACGAGTCTT+TGG 0.388380 6_1:-99013389 Msa0901910:CDS
CTGAAGACTTCAGAGACATT+TGG 0.388892 6_1:-99011126 Msa0901910:CDS
GGAACAGGAACTACGAATAA+AGG 0.399758 6_1:-99009618 Msa0901910:CDS
CTCCAAACTCCCCTTGATCT+TGG 0.403396 6_1:+99010967 None:intergenic
CAAAGCATGGTGAGCATCCT+TGG 0.404636 6_1:+99011826 None:intergenic
TCTGAAGTCTTCAGCACAAA+TGG 0.419146 6_1:+99011135 None:intergenic
TTGTATGCTATTAGACAGAT+TGG 0.429839 6_1:-99010895 Msa0901910:CDS
CAAGGATGCTCACCATGCTT+TGG 0.455806 6_1:-99011825 Msa0901910:CDS
TCTACCAGCTATCCGTCATA+TGG 0.457430 6_1:-99009825 Msa0901910:CDS
AAGTTCCTTGTTAAGAAACT+TGG 0.468839 6_1:+99009765 None:intergenic
CCGTCATATGGAGTTAGAAA+AGG 0.479711 6_1:-99009813 Msa0901910:CDS
AGATCAACAGAAGTGGGAAC+AGG 0.481464 6_1:-99009633 Msa0901910:CDS
TACCGACATCATAGTATAGT+TGG 0.481804 6_1:-99009723 Msa0901910:CDS
TGACGAGTCTTTGGCATCAC+TGG 0.484509 6_1:-99013380 Msa0901910:CDS
TGTGATGATGACCAAGATCA+AGG 0.491694 6_1:-99010978 Msa0901910:CDS
TTATTAGTCCAAACAAGCCT+TGG 0.494508 6_1:+99010934 None:intergenic
CTGAAAGATGTACAAGAGAA+AGG 0.494563 6_1:-99010065 Msa0901910:CDS
AATGACATGGAGATTATGTC+TGG 0.501152 6_1:+99011189 None:intergenic
TAGAAGCGACATCATAAAAT+GGG 0.505067 6_1:+99009844 None:intergenic
GCTATTAGACAGATTGGAAT+GGG 0.512204 6_1:-99010889 Msa0901910:CDS
GTACAAGAGAAAGGAATGGA+AGG 0.530188 6_1:-99010056 Msa0901910:CDS
TTTAAGTTCAAAATTTGCAT+CGG 0.531556 6_1:-99010015 Msa0901910:CDS
AGATGTACAAGAGAAAGGAA+TGG 0.533550 6_1:-99010060 Msa0901910:CDS
TGAATGTTCCAAACCTAACA+AGG 0.533568 6_1:-99010646 Msa0901910:CDS
AACTCCATATGACGGATAGC+TGG 0.539302 6_1:+99009821 None:intergenic
TGGAGTTAGAAAAGGAATGA+TGG 0.547753 6_1:-99009805 Msa0901910:CDS
TGCTATTAGACAGATTGGAA+TGG 0.548483 6_1:-99010890 Msa0901910:CDS
ATGCCAAAGACTCGTCATCA+TGG 0.549394 6_1:+99013386 None:intergenic
GTGATGATGACCAAGATCAA+GGG 0.559984 6_1:-99010977 Msa0901910:CDS
ATCACTGGCTAATATACTCA+TGG 0.566664 6_1:-99013365 Msa0901910:CDS
CAAGAATAGATCAACAGAAG+TGG 0.568819 6_1:-99009640 Msa0901910:CDS
TGTCGCCAAGTTTCTTAACA+AGG 0.569859 6_1:-99009770 Msa0901910:CDS
CAAGAGAAAGGAATGGAAGG+AGG 0.573386 6_1:-99010053 Msa0901910:CDS
AAGAATAGATCAACAGAAGT+GGG 0.573718 6_1:-99009639 Msa0901910:CDS
CACAACCAAAACTTGCATGT+TGG 0.574671 6_1:+99013411 None:intergenic
TTTGGAGTTGTAAGAAACCA+AGG 0.583346 6_1:-99010951 Msa0901910:CDS
CTCGAAGAGTGACCAAAGCA+TGG 0.605583 6_1:+99011813 None:intergenic
TGCTCCATGTTCTAATGACA+TGG 0.612114 6_1:+99011176 None:intergenic
GGTGTGAAGAACTGGAGCGT+TGG 0.621741 6_1:-99009672 Msa0901910:CDS
TTCCAACTATACTATGATGT+CGG 0.631257 6_1:+99009721 None:intergenic
ATCTCCATGTCATTAGAACA+TGG 0.631773 6_1:-99011180 Msa0901910:CDS
TGATGATGACCAAGATCAAG+GGG 0.634597 6_1:-99010976 Msa0901910:CDS
TGGCTAATATACTCATGGCA+TGG 0.635039 6_1:-99013360 Msa0901910:CDS
GAATGTTCCAAACCTAACAA+GGG 0.643998 6_1:-99010645 Msa0901910:CDS
CAGGAACTACGAATAAAGGA+AGG 0.649263 6_1:-99009614 Msa0901910:CDS
ATTAGACAGATTGGAATGGG+AGG 0.653232 6_1:-99010886 Msa0901910:intron
CTCATGGCATGGCAATACAA+AGG 0.657436 6_1:-99013349 Msa0901910:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTAACAAAACATTTTA+GGG + chr6_1:99010761-99010780 None:intergenic 10.0%
!!! TATATTTAACAAAACATTTT+AGG + chr6_1:99010762-99010781 None:intergenic 10.0%
!! AAAACCAAATAAAAAAAATT+AGG + chr6_1:99011135-99011154 None:intergenic 10.0%
!! TATAAAATAAAATAATTGCA+AGG + chr6_1:99011657-99011676 None:intergenic 10.0%
!!! AATAAATAGTTTATTTTTCT+TGG + chr6_1:99011915-99011934 None:intergenic 10.0%
!!! TATATAAAATGGAAAATTTT+AGG + chr6_1:99012597-99012616 None:intergenic 10.0%
!!! ACATATATTCTAAAAATATT+TGG + chr6_1:99012854-99012873 None:intergenic 10.0%
!! TTATATTTAATTAAGTAGTC+TGG + chr6_1:99010602-99010621 None:intergenic 15.0%
!! AAAACAAAGTTTAATATCAT+TGG - chr6_1:99010783-99010802 Msa0901910:intron 15.0%
!!! ATTATGAATAAACTTTTAAC+CGG - chr6_1:99010870-99010889 Msa0901910:intron 15.0%
!!! TTTTAACTTAAATTACTGAA+TGG + chr6_1:99011056-99011075 None:intergenic 15.0%
!!! ATGACCTAATTTTTTTTATT+TGG - chr6_1:99011128-99011147 Msa0901910:CDS 15.0%
!! TGGATGAAATTTAATAAATA+AGG - chr6_1:99011492-99011511 Msa0901910:intron 15.0%
!! GGATGAAATTTAATAAATAA+GGG - chr6_1:99011493-99011512 Msa0901910:intron 15.0%
!!! AATAAATAAGGGTATAATAA+TGG - chr6_1:99011504-99011523 Msa0901910:intron 15.0%
!!! ATTAGTCTTCATAAAAAAAA+AGG + chr6_1:99012478-99012497 None:intergenic 15.0%
!!! ACTGTTTTAAATAATAAATG+AGG + chr6_1:99012682-99012701 None:intergenic 15.0%
!! AAAATGAAAAAAATTAGAGT+AGG + chr6_1:99012827-99012846 None:intergenic 15.0%
!! AAAGTAATTCAATTATCTGA+TGG + chr6_1:99009698-99009717 None:intergenic 20.0%
!! ATTAAAATACCACAATTGAA+AGG + chr6_1:99009909-99009928 None:intergenic 20.0%
!!! TACAAATACTTTGTTTGTAA+AGG - chr6_1:99010116-99010135 Msa0901910:intron 20.0%
!!! AATCATTCTTTGTTTTTGAA+GGG + chr6_1:99010153-99010172 None:intergenic 20.0%
!!! AAATCATTCTTTGTTTTTGA+AGG + chr6_1:99010154-99010173 None:intergenic 20.0%
!!! TGGTATGTTAGTTTTTTTTT+AGG - chr6_1:99010803-99010822 Msa0901910:intron 20.0%
!!! TATGTTAGTTTTTTTTTAGG+AGG - chr6_1:99010806-99010825 Msa0901910:intron 20.0%
!!! ATGTTAGTTTTTTTTTAGGA+GGG - chr6_1:99010807-99010826 Msa0901910:intron 20.0%
!! TTATATATTTCTTCCTTATC+AGG + chr6_1:99010977-99010996 None:intergenic 20.0%
!! GAAGAAATATATAACTCTTA+TGG - chr6_1:99010983-99011002 Msa0901910:CDS 20.0%
!!! TTTCATATATTTGGAATGAA+TGG - chr6_1:99011244-99011263 Msa0901910:intron 20.0%
!! AAATGATATTTACCTATAAC+AGG + chr6_1:99011310-99011329 None:intergenic 20.0%
!!! ATTAATTGTGTTGATTTCAA+TGG - chr6_1:99011409-99011428 Msa0901910:intron 20.0%
!! CAAAATAATAAATGTTGCAT+TGG - chr6_1:99011530-99011549 Msa0901910:intron 20.0%
!!! TTAGTTAGCTTTCTATATAA+TGG + chr6_1:99012267-99012286 None:intergenic 20.0%
!!! AGACTAATGAAATTTTTTGA+AGG - chr6_1:99012490-99012509 Msa0901910:intron 20.0%
!! AGATATTCTAAATATTGTTG+AGG - chr6_1:99012514-99012533 Msa0901910:intron 20.0%
!! GTTATGGTGTATATATAAAA+TGG + chr6_1:99012608-99012627 None:intergenic 20.0%
!!! TTTAAGTTCAAAATTTGCAT+CGG - chr6_1:99012984-99013003 Msa0901910:intron 20.0%
!! TTAACAAGGAACTTAAAATT+TGG - chr6_1:99013243-99013262 Msa0901910:intron 20.0%
! AAACAGTAGAATACATTGTT+TGG + chr6_1:99009794-99009813 None:intergenic 25.0%
! ATTAATTATCAGAAGCCAAA+GGG + chr6_1:99009945-99009964 None:intergenic 25.0%
! AACCAATCCTTAAAATAAAG+GGG + chr6_1:99010188-99010207 None:intergenic 25.0%
! GAACCAATCCTTAAAATAAA+GGG + chr6_1:99010189-99010208 None:intergenic 25.0%
! TGAACCAATCCTTAAAATAA+AGG + chr6_1:99010190-99010209 None:intergenic 25.0%
! TCTATATGTATGAGTGAAAA+TGG - chr6_1:99010444-99010463 Msa0901910:intron 25.0%
! CTATATGTATGAGTGAAAAT+GGG - chr6_1:99010445-99010464 Msa0901910:intron 25.0%
!!! TGTTAGTTTTTTTTTAGGAG+GGG - chr6_1:99010808-99010827 Msa0901910:intron 25.0%
! TGAATCAAATTAACCTGATA+AGG - chr6_1:99010961-99010980 Msa0901910:CDS 25.0%
! AATATTAATGCACACTCAAT+AGG - chr6_1:99011019-99011038 Msa0901910:CDS 25.0%
! ATCAGTGAGTTTCATATATT+TGG - chr6_1:99011235-99011254 Msa0901910:intron 25.0%
!!! AGAGTATTTTTTAGTTGGAA+GGG + chr6_1:99011335-99011354 None:intergenic 25.0%
!!! TAGAGAGAGTATTTTTTAGT+TGG + chr6_1:99011340-99011359 None:intergenic 25.0%
! CTCTAAATTATAGTCGTTTA+AGG - chr6_1:99011361-99011380 Msa0901910:intron 25.0%
! CCACTAACTACAATTAATAA+GGG + chr6_1:99011461-99011480 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr6_1:99011462-99011481 None:intergenic 25.0%
!! CATCACTATTTGAAACAAAA+AGG + chr6_1:99011775-99011794 None:intergenic 25.0%
!!! TCATTATCTCCAAGATTTTT+TGG + chr6_1:99012336-99012355 None:intergenic 25.0%
! AAAAAAAACCTGTAAATGAC+TGG + chr6_1:99012389-99012408 None:intergenic 25.0%
! TACAAAAATAAACGGATAAC+AGG + chr6_1:99012630-99012649 None:intergenic 25.0%
! GTATGCAAAGAATATTATGT+TGG - chr6_1:99012702-99012721 Msa0901910:intron 25.0%
!! ATGAAATTGGTAACTGAATT+TGG + chr6_1:99012799-99012818 None:intergenic 25.0%
! AGAGTAGGAAAAAATGAAAT+TGG + chr6_1:99012812-99012831 None:intergenic 25.0%
!! ATTTCATTGATGACAGAAAT+TGG + chr6_1:99013015-99013034 None:intergenic 25.0%
! TCGTTCAATCATTTCAATTA+TGG - chr6_1:99013306-99013325 Msa0901910:intron 25.0%
! AATGTTATTCCATATAGCTT+TGG - chr6_1:99013414-99013433 Msa0901910:CDS 25.0%
! ATGTTATTCCATATAGCTTT+GGG - chr6_1:99013415-99013434 Msa0901910:CDS 25.0%
CTGATGGTTAAATAGAAAAG+AGG + chr6_1:99009682-99009701 None:intergenic 30.0%
! AAAGTTTTGCCTTTCAATTG+TGG - chr6_1:99009897-99009916 Msa0901910:CDS 30.0%
ATTGTTAGATATCAACCCTT+TGG - chr6_1:99009927-99009946 Msa0901910:CDS 30.0%
GATTAATTATCAGAAGCCAA+AGG + chr6_1:99009946-99009965 None:intergenic 30.0%
! ATCTTGGCTAAAGCAAATTT+TGG + chr6_1:99010005-99010024 None:intergenic 30.0%
! AATTTGCTTTAGCCAAGATT+TGG - chr6_1:99010006-99010025 Msa0901910:CDS 30.0%
ACCAATCCTTAAAATAAAGG+GGG + chr6_1:99010187-99010206 None:intergenic 30.0%
AGACAATCAAAAGGGAAAAA+AGG + chr6_1:99010410-99010429 None:intergenic 30.0%
!! AAGTGTTGAGACAATCAAAA+GGG + chr6_1:99010418-99010437 None:intergenic 30.0%
!! AAAGTGTTGAGACAATCAAA+AGG + chr6_1:99010419-99010438 None:intergenic 30.0%
!! TTTTCCGTTTTAAACTGAGA+AGG - chr6_1:99010528-99010547 Msa0901910:intron 30.0%
! TTAGCCTTCTCAGTTTAAAA+CGG + chr6_1:99010535-99010554 None:intergenic 30.0%
! TAATTAAGTAGTCTGGTTTG+TGG + chr6_1:99010595-99010614 None:intergenic 30.0%
!!! GTTAGTTTTTTTTTAGGAGG+GGG - chr6_1:99010809-99010828 Msa0901910:intron 30.0%
!!! TTAGTTTTTTTTTAGGAGGG+GGG - chr6_1:99010810-99010829 Msa0901910:intron 30.0%
! AGAATAAGTTGAGCAAAGTT+AGG + chr6_1:99010918-99010937 None:intergenic 30.0%
! TATTCTCAAACAGGAGTTTT+AGG - chr6_1:99010932-99010951 Msa0901910:CDS 30.0%
!! ACTCATCTTGAATTTGCTTT+TGG + chr6_1:99011279-99011298 None:intergenic 30.0%
!!! GAGTATTTTTTAGTTGGAAG+GGG + chr6_1:99011334-99011353 None:intergenic 30.0%
!!! GAGAGTATTTTTTAGTTGGA+AGG + chr6_1:99011336-99011355 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr6_1:99011458-99011477 Msa0901910:intron 30.0%
TTATAGCAAGTGAAGAAAAG+AGG - chr6_1:99011945-99011964 Msa0901910:intron 30.0%
TTGTATGCTATTAGACAGAT+TGG - chr6_1:99012104-99012123 Msa0901910:intron 30.0%
CATCTGAAAGAGAGAAAAAA+AGG + chr6_1:99012308-99012327 None:intergenic 30.0%
AAATGTATCCAGTCATTTAC+AGG - chr6_1:99012378-99012397 Msa0901910:intron 30.0%
AATAAACGGATAACAGGTTA+TGG + chr6_1:99012624-99012643 None:intergenic 30.0%
! TTTTCGCTTGAAAAAGTGAA+AGG + chr6_1:99013071-99013090 None:intergenic 30.0%
TAGAAGCGACATCATAAAAT+GGG + chr6_1:99013158-99013177 None:intergenic 30.0%
AAGTTCCTTGTTAAGAAACT+TGG + chr6_1:99013237-99013256 None:intergenic 30.0%
TTCCAACTATACTATGATGT+CGG + chr6_1:99013281-99013300 None:intergenic 30.0%
AAGAATAGATCAACAGAAGT+GGG - chr6_1:99013360-99013379 Msa0901910:CDS 30.0%
! ATCACTGGCTAATATACTCA+TGG - chr6_1:99009634-99009653 Msa0901910:CDS 35.0%
AGATTTGCAATGCATACACA+AGG + chr6_1:99009827-99009846 None:intergenic 35.0%
AGACACTAGATTCCAAATCT+TGG + chr6_1:99010021-99010040 None:intergenic 35.0%
!!! TCCCCCTTTATTTTAAGGAT+TGG - chr6_1:99010183-99010202 Msa0901910:intron 35.0%
!!! ATTTTAAGGATTGGTTCACG+TGG - chr6_1:99010192-99010211 Msa0901910:intron 35.0%
CTACATTGTAGGATTCTCAA+GGG + chr6_1:99010479-99010498 None:intergenic 35.0%
!!! TAGTTTTTTTTTAGGAGGGG+GGG - chr6_1:99010811-99010830 Msa0901910:intron 35.0%
! CTTTTAACCGGACTATACTT+AGG - chr6_1:99010882-99010901 Msa0901910:intron 35.0%
TGCTCAACTTATTCTCAAAC+AGG - chr6_1:99010923-99010942 Msa0901910:CDS 35.0%
GAGTTTCAACTACCTGTTAT+AGG - chr6_1:99011295-99011314 Msa0901910:intron 35.0%
AGTTAGTGGAGTAGTTAAGT+TGG - chr6_1:99011472-99011491 Msa0901910:intron 35.0%
AGACAATTATTGTGAGACGT+AGG - chr6_1:99011600-99011619 Msa0901910:intron 35.0%
GACAATTATTGTGAGACGTA+GGG - chr6_1:99011601-99011620 Msa0901910:intron 35.0%
AATGACATGGAGATTATGTC+TGG + chr6_1:99011813-99011832 None:intergenic 35.0%
ATCTCCATGTCATTAGAACA+TGG - chr6_1:99011819-99011838 Msa0901910:CDS 35.0%
! TTTGGAGTTGTAAGAAACCA+AGG - chr6_1:99012048-99012067 Msa0901910:intron 35.0%
TTATTAGTCCAAACAAGCCT+TGG + chr6_1:99012068-99012087 None:intergenic 35.0%
TGCTATTAGACAGATTGGAA+TGG - chr6_1:99012109-99012128 Msa0901910:intron 35.0%
GCTATTAGACAGATTGGAAT+GGG - chr6_1:99012110-99012129 Msa0901910:intron 35.0%
GATGTTGTGCCAAAAAATCT+TGG - chr6_1:99012324-99012343 Msa0901910:intron 35.0%
TGAATGTTCCAAACCTAACA+AGG - chr6_1:99012353-99012372 Msa0901910:intron 35.0%
GAATGTTCCAAACCTAACAA+GGG - chr6_1:99012354-99012373 Msa0901910:intron 35.0%
! ACATTTTCCCTTGTTAGGTT+TGG + chr6_1:99012364-99012383 None:intergenic 35.0%
! TGGATACATTTTCCCTTGTT+AGG + chr6_1:99012369-99012388 None:intergenic 35.0%
!! GAATATTATGTTGGTTCGAG+TGG - chr6_1:99012711-99012730 Msa0901910:intron 35.0%
CTGAAAGATGTACAAGAGAA+AGG - chr6_1:99012934-99012953 Msa0901910:intron 35.0%
AGATGTACAAGAGAAAGGAA+TGG - chr6_1:99012939-99012958 Msa0901910:intron 35.0%
GCGAAAAACTCTTTCAATGA+TGG - chr6_1:99013084-99013103 Msa0901910:intron 35.0%
! TTTTCGCTTGAAAAGGTGAA+AGG + chr6_1:99013134-99013153 None:intergenic 35.0%
!! AATGGGTTTTTCGCTTGAAA+AGG + chr6_1:99013141-99013160 None:intergenic 35.0%
GTAGAAGCGACATCATAAAA+TGG + chr6_1:99013159-99013178 None:intergenic 35.0%
! CCTTTTCTAACTCCATATGA+CGG + chr6_1:99013189-99013208 None:intergenic 35.0%
TGGAGTTAGAAAAGGAATGA+TGG - chr6_1:99013194-99013213 Msa0901910:intron 35.0%
TACCGACATCATAGTATAGT+TGG - chr6_1:99013276-99013295 Msa0901910:intron 35.0%
TCAATTATGGTGTGAAGAAC+TGG - chr6_1:99013319-99013338 Msa0901910:intron 35.0%
CAAGAATAGATCAACAGAAG+TGG - chr6_1:99013359-99013378 Msa0901910:CDS 35.0%
!! TCCATATAGCTTTGGGTTAA+TGG - chr6_1:99013422-99013441 Msa0901910:CDS 35.0%
TCCATTAACCCAAAGCTATA+TGG + chr6_1:99013426-99013445 None:intergenic 35.0%
! CAGATCCAACATGCAAGTTT+TGG - chr6_1:99009583-99009602 Msa0901910:CDS 40.0%
CACAACCAAAACTTGCATGT+TGG + chr6_1:99009591-99009610 None:intergenic 40.0%
!!! CAACATGCAAGTTTTGGTTG+TGG - chr6_1:99009589-99009608 Msa0901910:CDS 40.0%
TGGCTAATATACTCATGGCA+TGG - chr6_1:99009639-99009658 Msa0901910:CDS 40.0%
TAATCCAGAATCTAGCTGAG+AGG - chr6_1:99009961-99009980 Msa0901910:CDS 40.0%
!!! TGCGATCCCCCTTTATTTTA+AGG - chr6_1:99010178-99010197 Msa0901910:intron 40.0%
TGACACAATTCAAAGACTCG+AGG + chr6_1:99010347-99010366 None:intergenic 40.0%
GCTACATTGTAGGATTCTCA+AGG + chr6_1:99010480-99010499 None:intergenic 40.0%
AGAATCCTACAATGTAGCCT+AGG - chr6_1:99010482-99010501 Msa0901910:intron 40.0%
AACTTGATCTGATCTGACCT+AGG + chr6_1:99010502-99010521 None:intergenic 40.0%
CACAATGCCTAAGTATAGTC+CGG + chr6_1:99010892-99010911 None:intergenic 40.0%
! ATTTTCAGTTCGAACTGCCA+AGG - chr6_1:99011156-99011175 Msa0901910:CDS 40.0%
TGCTCCATGTTCTAATGACA+TGG + chr6_1:99011826-99011845 None:intergenic 40.0%
! TCTGAAGTCTTCAGCACAAA+TGG + chr6_1:99011867-99011886 None:intergenic 40.0%
CTGAAGACTTCAGAGACATT+TGG - chr6_1:99011873-99011892 Msa0901910:intron 40.0%
! AGACATTTGGAAGTATGCAC+TGG - chr6_1:99011886-99011905 Msa0901910:intron 40.0%
! TGTGATGATGACCAAGATCA+AGG - chr6_1:99012021-99012040 Msa0901910:intron 40.0%
! GTGATGATGACCAAGATCAA+GGG - chr6_1:99012022-99012041 Msa0901910:intron 40.0%
! TGATGATGACCAAGATCAAG+GGG - chr6_1:99012023-99012042 Msa0901910:intron 40.0%
! GTAAGAAACCAAGGCTTGTT+TGG - chr6_1:99012057-99012076 Msa0901910:intron 40.0%
ATTAGACAGATTGGAATGGG+AGG - chr6_1:99012113-99012132 Msa0901910:intron 40.0%
GTACAAGAGAAAGGAATGGA+AGG - chr6_1:99012943-99012962 Msa0901910:intron 40.0%
CCGTCATATGGAGTTAGAAA+AGG - chr6_1:99013186-99013205 Msa0901910:intron 40.0%
TGTCGCCAAGTTTCTTAACA+AGG - chr6_1:99013229-99013248 Msa0901910:intron 40.0%
GGAACAGGAACTACGAATAA+AGG - chr6_1:99013381-99013400 Msa0901910:CDS 40.0%
CAGGAACTACGAATAAAGGA+AGG - chr6_1:99013385-99013404 Msa0901910:CDS 40.0%
ATGCCAAAGACTCGTCATCA+TGG + chr6_1:99009616-99009635 None:intergenic 45.0%
CTCATGGCATGGCAATACAA+AGG - chr6_1:99009650-99009669 Msa0901910:CDS 45.0%
TCAACCTCTCAGCTAGATTC+TGG + chr6_1:99009968-99009987 None:intergenic 45.0%
!!! AGGATTGGTTCACGTGGTTT+TGG - chr6_1:99010198-99010217 Msa0901910:intron 45.0%
TCTGACCTAGGCTACATTGT+AGG + chr6_1:99010490-99010509 None:intergenic 45.0%
! TAAGTAGTCTGGTTTGTGGC+AGG + chr6_1:99010591-99010610 None:intergenic 45.0%
CAAGAGAAAGGAATGGAAGG+AGG - chr6_1:99012946-99012965 Msa0901910:intron 45.0%
TCTACCAGCTATCCGTCATA+TGG - chr6_1:99013174-99013193 Msa0901910:intron 45.0%
!! AACTCCATATGACGGATAGC+TGG + chr6_1:99013181-99013200 None:intergenic 45.0%
AGATCAACAGAAGTGGGAAC+AGG - chr6_1:99013366-99013385 Msa0901910:CDS 45.0%
!!! TTTTTTTTTAAAGTTTATTT+AGG - chr6_1:99010557-99010576 Msa0901910:intron 5.0%
!!! TAAAATATTCAATATTTTAA+TGG - chr6_1:99011071-99011090 Msa0901910:intron 5.0%
!! ATATATTATACAAAAATAAA+CGG + chr6_1:99012638-99012657 None:intergenic 5.0%
! GGACCATGATGACGAGTCTT+TGG - chr6_1:99009610-99009629 Msa0901910:CDS 50.0%
TGACGAGTCTTTGGCATCAC+TGG - chr6_1:99009619-99009638 Msa0901910:CDS 50.0%
CAAAGCATGGTGAGCATCCT+TGG + chr6_1:99011176-99011195 None:intergenic 50.0%
CAAGGATGCTCACCATGCTT+TGG - chr6_1:99011174-99011193 Msa0901910:CDS 50.0%
CTCGAAGAGTGACCAAAGCA+TGG + chr6_1:99011189-99011208 None:intergenic 50.0%
GACCAAGATCAAGGGGAGTT+TGG - chr6_1:99012030-99012049 Msa0901910:intron 50.0%
CTCCAAACTCCCCTTGATCT+TGG + chr6_1:99012035-99012054 None:intergenic 50.0%
GACAGATTGGAATGGGAGGT+AGG - chr6_1:99012117-99012136 Msa0901910:intron 50.0%
GGTGTGAAGAACTGGAGCGT+TGG - chr6_1:99013327-99013346 Msa0901910:intron 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 99009572 99013449 99009572 ID=Msa0901910;Name=Msa0901910
chr6_1 mRNA 99009572 99013449 99009572 ID=Msa0901910-mRNA-1;Parent=Msa0901910;Name=Msa0901910-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|0.83|0.8|0.83|6|0|352
chr6_1 exon 99013350 99013449 99013350 ID=Msa0901910-mRNA-1:exon:17523;Parent=Msa0901910-mRNA-1
chr6_1 exon 99011783 99011857 99011783 ID=Msa0901910-mRNA-1:exon:17522;Parent=Msa0901910-mRNA-1
chr6_1 exon 99011124 99011231 99011124 ID=Msa0901910-mRNA-1:exon:17521;Parent=Msa0901910-mRNA-1
chr6_1 exon 99010887 99011056 99010887 ID=Msa0901910-mRNA-1:exon:17520;Parent=Msa0901910-mRNA-1
chr6_1 exon 99010622 99010696 99010622 ID=Msa0901910-mRNA-1:exon:17519;Parent=Msa0901910-mRNA-1
chr6_1 exon 99009572 99010102 99009572 ID=Msa0901910-mRNA-1:exon:17518;Parent=Msa0901910-mRNA-1
chr6_1 CDS 99013350 99013449 99013350 ID=Msa0901910-mRNA-1:cds;Parent=Msa0901910-mRNA-1
chr6_1 CDS 99011783 99011857 99011783 ID=Msa0901910-mRNA-1:cds;Parent=Msa0901910-mRNA-1
chr6_1 CDS 99011124 99011231 99011124 ID=Msa0901910-mRNA-1:cds;Parent=Msa0901910-mRNA-1
chr6_1 CDS 99010887 99011056 99010887 ID=Msa0901910-mRNA-1:cds;Parent=Msa0901910-mRNA-1
chr6_1 CDS 99010622 99010696 99010622 ID=Msa0901910-mRNA-1:cds;Parent=Msa0901910-mRNA-1
chr6_1 CDS 99009572 99010102 99009572 ID=Msa0901910-mRNA-1:cds;Parent=Msa0901910-mRNA-1
Gene Sequence

>Msa0901910

ATGAGTTACGACAGATCCAACATGCAAGTTTTGGTTGTGGACCATGATGACGAGTCTTTGGCATCACTGGCTAATATACTCATGGCATGGCAATACAAAGTTCGAACTGCCAAGGATGCTCACCATGCTTTGGTCACTCTTCGAGACTCTCAAGTCTTGTTTGATCTCATAATCATGATGTCTAAAGATAATAGACCAGACATAATCTCCATGTCATTAGAACATGGAGCAGCACAATATATTGTTAAACCATTTGTGCTGAAGACTTCAGAGACATTTGGAAAGGAGACATCAACTGATCAATTACAAACTAAAAAGAATTGTAGTAAAAGAAAGAGTTGTGATGATGACCAAGATCAAGGGGAGTTTGGAGTTGTAAGAAACCAAGGCTTGTTTGGACTAATAATCTTCACAGTCGATTTTTGTATGCTATTAGACAGATTGGAATGGGAGATGTTGTGCCAAAAAATCTTGGAGATAATGAATGTTCCAAACCTAACAAGGGAAAATGTATCCAGTCATTTACAGAAGCATCGCATGTTTCTGAAAGATGTACAAGAGAAAGGAATGGAAGGAGGAGTTTCTCAGAGAGCTTTAAGTTCAAAATTTGCATCGGATCTTCCAATTTCTGTCATCAATGAAATCATTGAAAGAAGAAAAAACAGTCGATATTCAAACCTTTCACTTTTTCAAGCGAAAAACTCTTTCAATGATGGTGCTTCTACTAGCAATTCATCAAACCTTTCACCTTTTCAAGCGAAAAACCCATTTTATGATGTCGCTTCTACCAGCTATCCGTCATATGGAGTTAGAAAAGGAATGATGGCTTCTTCTTCTTTGTCGCCAAGTTTCTTAACAAGGAACTTAAAATTTGGTGATACTAGTTACCGACATCATAGTATAGTTGGAAATGATTCGTTCAATCATTTCAATTATGGTGTGAAGAACTGGAGCGTTGGATCTACAATCAAGAATAGATCAACAGAAGTGGGAACAGGAACTACGAATAAAGGAAGGTTTGATAATGTTATTCCATATAGCTTTGGGTTAATGGAGTAA
Protein sequence

>Msa0901910

MSYDRSNMQVLVVDHDDESLASLANILMAWQYKVRTAKDAHHALVTLRDSQVLFDLIIMMSKDNRPDIISMSLEHGAAQYIVKPFVLKTSETFGKETSTDQLQTKKNCSKRKSCDDDQDQGEFGVVRNQGLFGLIIFTVDFCMLLDRLEWEMLCQKILEIMNVPNLTRENVSSHLQKHRMFLKDVQEKGMEGGVSQRALSSKFASDLPISVINEIIERRKNSRYSNLSLFQAKNSFNDGASTSNSSNLSPFQAKNPFYDVASTSYPSYGVRKGMMASSSLSPSFLTRNLKFGDTSYRHHSIVGNDSFNHFNYGVKNWSVGSTIKNRSTEVGTGTTNKGRFDNVIPYSFGLME*