AlfalfaGEDB | Msa0913360

M. sativa cultivar zhongmu-4 / Msa0913360

KEGG: K09419
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0913360	XP_013452015.1	71.779	326	75	5	118	431	7	327	2.52e-158	460
Msa0913360	XP_013452015.1	80.952	42	8	0	2	43	87	128	6.91e-14	84.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0913360	sp\|Q94BZ5\|HSFA5_ARATH	58.294	211	87	1	133	342	24	234	4.88e-81	260
Msa0913360	sp\|Q94BZ5\|HSFA5_ARATH	50.000	68	33	1	1	67	87	154	3.36e-13	74.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0913360	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0913360	MtrunA17_Chr6g0467041	71.779	326	75	5	118	431	7	327	2.32e-162	460
Msa0913360	MtrunA17_Chr6g0467041	80.952	42	8	0	2	43	87	128	6.36e-18	84.3
Msa0913360	MtrunA17_Chr8g0378061	76.871	294	29	4	133	387	23	316	2.07e-151	438
Msa0913360	MtrunA17_Chr8g0378061	61.538	65	24	1	2	65	87	151	5.45e-18	85.9
Msa0913360	MtrunA17_Chr8g0378061	82.609	46	8	0	388	433	412	457	8.24e-17	82.4
Msa0913360	MtrunA17_Chr8g0378061	46.970	66	35	0	68	133	393	458	2.49e-12	68.6
Msa0913360	MtrunA17_Chr2g0330821	56.318	277	101	3	120	379	67	340	5.54e-96	292
Msa0913360	MtrunA17_Chr2g0330821	54.545	66	29	1	1	65	143	208	7.15e-14	72.8
Msa0913360	MtrunA17_Chr2g0330841	52.362	254	75	6	174	387	29	276	5.14e-71	229
Msa0913360	MtrunA17_Chr2g0330841	78.571	42	9	0	2	43	46	87	1.26e-16	81.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0913360	AT4G13980.1	58.294	211	87	1	133	342	24	234	4.96e-82	260
Msa0913360	AT4G13980.1	50.000	68	33	1	1	67	87	154	3.41e-14	74.7
Msa0913360	AT4G17750.1	48.611	216	90	4	132	326	52	267	6.87e-57	195
Msa0913360	AT5G16820.1	49.289	211	92	5	130	326	25	234	1.32e-56	194
Msa0913360	AT5G16820.2	49.289	211	92	5	130	326	25	234	1.32e-56	194
Msa0913360	AT1G32330.2	49.533	214	89	5	132	326	37	250	3.80e-56	193
Msa0913360	AT1G32330.1	49.533	214	89	5	132	326	37	250	4.09e-56	193
Msa0913360	AT3G02990.1	45.701	221	107	4	130	338	21	240	4.65e-56	192
Msa0913360	AT5G45710.3	34.756	328	167	6	124	429	5	307	2.81e-50	174
Msa0913360	AT5G45710.1	34.756	328	167	6	124	429	5	307	2.81e-50	174

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Find 99 sgRNAs with CRISPR-Local

Find 804 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGATCCTGATCGTT+GGG	0.473476	6_2:+24062117	Msa0913360:intron
AAAATCAGATCCTGATCGTT+GGG	0.473476	6_2:+24071857	Msa0913360:CDS
AAATAAACTCCCTGAAGATG+AGG	0.580535	6_2:+24072328	Msa0913360:CDS
AAATAAACTCCCTGAAGATG+AGG	0.582708	6_2:+24063163	Msa0913360:CDS
AACAGCATGGACTTTCAGTA+AGG	0.541453	6_2:-24063358	None:intergenic
AACTGACCAAGCAATTACTG+GGG	0.734160	6_2:+24063238	Msa0913360:CDS
AACTGACCAAGCAATTACTG+GGG	0.734160	6_2:+24072403	Msa0913360:CDS
AAGATGCCCCAGTAATTGCT+TGG	0.581078	6_2:-24072409	None:intergenic
AAGGTAGCATGGTTAATCCA+TGG	0.570861	6_2:+24072285	Msa0913360:CDS
AAGGTAGCTTGGTTAATCCA+TGG	0.568191	6_2:+24063120	Msa0913360:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGGCAATGAGTAAAAACTA+AGG	+	chr6_2:24060535-24060554	Msa0913360:intron	30.0%
TCTCTATGAAATCTTTGACA+GGG	-	chr6_2:24060708-24060727	None:intergenic	30.0%
TTCTCTATGAAATCTTTGAC+AGG	-	chr6_2:24060709-24060728	None:intergenic	30.0%
ATTTCATAGAGAACTCATCT+TGG	+	chr6_2:24060716-24060735	Msa0913360:intron	30.0%
AATTAGCTGGTAAATATGAG+GGG	-	chr6_2:24060770-24060789	None:intergenic	30.0%
GAATTAGCTGGTAAATATGA+GGG	-	chr6_2:24060771-24060790	None:intergenic	30.0%
TGAATTAGCTGGTAAATATG+AGG	-	chr6_2:24060772-24060791	None:intergenic	30.0%
AAATCTGTTTGTCTTCAAGA+GGG	-	chr6_2:24060816-24060835	None:intergenic	30.0%
GATGACAAGTTAATGGAAAA+AGG	+	chr6_2:24060997-24061016	Msa0913360:intron	30.0%
TGGATGAAAGTTAATACTGA+TGG	+	chr6_2:24061474-24061493	Msa0913360:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	24059841	24072577	24059841	ID=Msa0913360;Name=Msa0913360
chr6_2	mRNA	24059841	24072577	24059841	ID=Msa0913360-mRNA-1;Parent=Msa0913360;Name=Msa0913360-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|0\|0\|0.8\|0.25\|0.2\|5\|0\|433
chr6_2	exon	24059841	24059844	24059841	ID=Msa0913360-mRNA-1:exon:4686;Parent=Msa0913360-mRNA-1
chr6_2	exon	24062125	24062232	24062125	ID=Msa0913360-mRNA-1:exon:4687;Parent=Msa0913360-mRNA-1
chr6_2	exon	24063123	24063368	24063123	ID=Msa0913360-mRNA-1:exon:4688;Parent=Msa0913360-mRNA-1
chr6_2	exon	24070327	24070541	24070327	ID=Msa0913360-mRNA-1:exon:4689;Parent=Msa0913360-mRNA-1
chr6_2	exon	24071849	24072577	24071849	ID=Msa0913360-mRNA-1:exon:4690;Parent=Msa0913360-mRNA-1
chr6_2	CDS	24059841	24059844	24059841	ID=Msa0913360-mRNA-1:cds;Parent=Msa0913360-mRNA-1
chr6_2	CDS	24062125	24062232	24062125	ID=Msa0913360-mRNA-1:cds;Parent=Msa0913360-mRNA-1
chr6_2	CDS	24063123	24063368	24063123	ID=Msa0913360-mRNA-1:cds;Parent=Msa0913360-mRNA-1
chr6_2	CDS	24070327	24070541	24070327	ID=Msa0913360-mRNA-1:cds;Parent=Msa0913360-mRNA-1
chr6_2	CDS	24071849	24072577	24071849	ID=Msa0913360-mRNA-1:cds;Parent=Msa0913360-mRNA-1

Gene Sequence

>Msa0913360

ATGGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAAACATAGTCATCCTCCGGGTAGCTTGGTTAATCCATGGAATGTTGTCGAAGATATATCAAATAAACTCCCTGAAGATGAGGAAAGCCTGCAGAAAAATCTTTCTAAAGGAGAACTGACAGGAATGCAGACAAGAACTGACCAAGCAATTACTGGGGCATCTATTAATCTAGCTAATCCAAAGGAGGCTTCTGGTAACATACAAGTCGAAGCAGCTGCTCGGAACGGAGTGAATGATGTGTTTTGGGAACAATTCCTTACTGAAACCGCTGCTGCCGACGGAAGAAGAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAACCTATTTCAAACACAACAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGGATTTCGAAAATCAGATCCTGATCGTTGGGAGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTACTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTTTCGCGCGAAAAATGCCCTTCAATCCAAACTCCTAAGCTATAATTATCTTGAGACTGAGAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGATGGCATGGAGAAGAGGCAGACAAATTTGCAGAACTTCTTTGAGAAGGCTCTTCAAGATTCTTTTATTGTTGAACTTCTTTCCCACAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCATGGTTAATCCATGGAATATTGCCGAAGATATCTCAAATAAACTCCCTGAAGATGAGGAAAGCCTGCAGAAAAATCTTTCTGACGGAGAACTGATAGGAATGCAGACAAGAACTGACCAAGCAATTACTGGGGCATCTTTTACTAATGTGGCTAATCCAAAGGAGGCTTCAGGAAACGTCCAAGTCGAAGCAGCTGCTCGGCACGGAGTGAATGATGTCTTTTGGGAACAATTTCTTACTGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATCTTCAACTAA

Protein sequence

>Msa0913360

MDPDRWEFANEKFIKDQKHLLTDIHRKKPIHKHSHPPGSLVNPWNVVEDISNKLPEDEESLQKNLSKGELTGMQTRTDQAITGASINLANPKEASGNIQVEAAARNGVNDVFWEQFLTETAAADGRREGMAPFLQKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVLLPTYFKHNNFSSFIRQLNTYGFRKSDPDRWEFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSTVDPERAALEKEIEKLFRAKNALQSKLLSYNYLETEKLQLEDFQRRLDGMEKRQTNLQNFFEKALQDSFIVELLSHKIESMDLETYNKKRRLSQVDHMQPVAEGSMVNPWNIAEDISNKLPEDEESLQKNLSDGELIGMQTRTDQAITGASFTNVANPKEASGNVQVEAAARHGVNDVFWEQFLTESPCCSDNEEEIFN*