Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0915270 | KEH26442.1 | 81.250 | 80 | 7 | 1 | 4 | 75 | 75 | 154 | 6.86e-37 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0915270 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 6 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTATCATCCGTCTTATT+TGG | 0.197268 | 6_2:+31273331 | None:intergenic |
| TCCAAATAAGACGGATGATA+AGG | 0.391979 | 6_2:-31273332 | Msa0915270:CDS |
| TCAAATACTTGTCCAATCTT+AGG | 0.416494 | 6_2:+31273493 | None:intergenic |
| TATGAATGTGTGCCTAAGAT+TGG | 0.464884 | 6_2:-31273505 | Msa0915270:CDS |
| GCAAAGTGAATCTATTGTCT+AGG | 0.527263 | 6_2:-31273308 | None:intergenic |
| GTATTTGATACGTTACAAGA+AGG | 0.622453 | 6_2:-31273478 | Msa0915270:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTATTTTTTTGACAA+AGG | + | chr6_2:31273681-31273700 | None:intergenic | 10.0% |
| !!! | ATTTTTTATTTTTTTGACAA+AGG | + | chr6_2:31273681-31273700 | None:intergenic | 10.0% |
| !! | ATAGAAAAAAGACATATTTA+AGG | + | chr6_2:31273609-31273628 | None:intergenic | 15.0% |
| !!! | TAACTTTTTTTTCTTTTCAT+GGG | - | chr6_2:31273712-31273731 | Msa0915270:intron | 15.0% |
| !! | ATAGAAAAAAGACATATTTA+AGG | + | chr6_2:31273609-31273628 | None:intergenic | 15.0% |
| !!! | TAACTTTTTTTTCTTTTCAT+GGG | - | chr6_2:31273712-31273731 | Msa0915270:intron | 15.0% |
| !! | AAAGATACATCAATTTGATT+TGG | - | chr6_2:31273438-31273457 | Msa0915270:CDS | 20.0% |
| !! | TACTAATTATAATATACCTG+AGG | + | chr6_2:31273536-31273555 | None:intergenic | 20.0% |
| !! | CAGGTATATTATAATTAGTA+TGG | - | chr6_2:31273536-31273555 | Msa0915270:intron | 20.0% |
| !! | TGAATATGATCAATATAACA+AGG | + | chr6_2:31273576-31273595 | None:intergenic | 20.0% |
| !! | TAACTCAATAGTTAAATATG+AGG | + | chr6_2:31273658-31273677 | None:intergenic | 20.0% |
| !!! | GTAACTTTTTTTTCTTTTCA+TGG | - | chr6_2:31273711-31273730 | Msa0915270:intron | 20.0% |
| !! | ATGATATTGGGTTTATATAT+AGG | - | chr6_2:31273735-31273754 | Msa0915270:intron | 20.0% |
| !!! | TTTTCTTTCTATTTTCTTGT+TGG | - | chr6_2:31273776-31273795 | Msa0915270:intron | 20.0% |
| !! | AAAGATACATCAATTTGATT+TGG | - | chr6_2:31273438-31273457 | Msa0915270:CDS | 20.0% |
| !! | TACTAATTATAATATACCTG+AGG | + | chr6_2:31273536-31273555 | None:intergenic | 20.0% |
| !! | CAGGTATATTATAATTAGTA+TGG | - | chr6_2:31273536-31273555 | Msa0915270:intron | 20.0% |
| !! | TGAATATGATCAATATAACA+AGG | + | chr6_2:31273576-31273595 | None:intergenic | 20.0% |
| !! | TAACTCAATAGTTAAATATG+AGG | + | chr6_2:31273658-31273677 | None:intergenic | 20.0% |
| !!! | GTAACTTTTTTTTCTTTTCA+TGG | - | chr6_2:31273711-31273730 | Msa0915270:intron | 20.0% |
| !! | ATGATATTGGGTTTATATAT+AGG | - | chr6_2:31273735-31273754 | Msa0915270:intron | 20.0% |
| !!! | TTTTCTTTCTATTTTCTTGT+TGG | - | chr6_2:31273776-31273795 | Msa0915270:intron | 20.0% |
| ! | GATTTAATTCTCTCATTAGT+AGG | - | chr6_2:31273317-31273336 | Msa0915270:CDS | 25.0% |
| ! | TGGAATAATAAACGTTATGA+GGG | + | chr6_2:31273388-31273407 | None:intergenic | 25.0% |
| ! | ATGGAATAATAAACGTTATG+AGG | + | chr6_2:31273389-31273408 | None:intergenic | 25.0% |
| ! | AAATATGAGGAGATATAACA+AGG | + | chr6_2:31273645-31273664 | None:intergenic | 25.0% |
| !! | TGTTGTTTATATCTTGTTGA+TGG | - | chr6_2:31273875-31273894 | Msa0915270:intron | 25.0% |
| !!! | AGGTTATTTTCCAAATAAGA+CGG | - | chr6_2:31274114-31274133 | Msa0915270:intron | 25.0% |
| ! | GATTTAATTCTCTCATTAGT+AGG | - | chr6_2:31273317-31273336 | Msa0915270:CDS | 25.0% |
| ! | TGGAATAATAAACGTTATGA+GGG | + | chr6_2:31273388-31273407 | None:intergenic | 25.0% |
| ! | ATGGAATAATAAACGTTATG+AGG | + | chr6_2:31273389-31273408 | None:intergenic | 25.0% |
| ! | AAATATGAGGAGATATAACA+AGG | + | chr6_2:31273645-31273664 | None:intergenic | 25.0% |
| !! | TGTTGTTTATATCTTGTTGA+TGG | - | chr6_2:31273875-31273894 | Msa0915270:intron | 25.0% |
| !!! | AGGTTATTTTCCAAATAAGA+CGG | - | chr6_2:31274114-31274133 | Msa0915270:intron | 25.0% |
| GTTGTGTTTATCATTCTTCA+TGG | - | chr6_2:31273481-31273500 | Msa0915270:CDS | 30.0% | |
| !! | AACTTGTTTTCAAATTCCTC+AGG | - | chr6_2:31273517-31273536 | Msa0915270:intron | 30.0% |
| ! | TTCTTTTCATGGGATGATAT+TGG | - | chr6_2:31273722-31273741 | Msa0915270:intron | 30.0% |
| ! | TCTTTTCATGGGATGATATT+GGG | - | chr6_2:31273723-31273742 | Msa0915270:intron | 30.0% |
| !!! | TTGGTTTTATTGCTGTTCAA+TGG | - | chr6_2:31273795-31273814 | Msa0915270:intron | 30.0% |
| !! | TTTTATTGCTGTTCAATGGT+GGG | - | chr6_2:31273799-31273818 | Msa0915270:intron | 30.0% |
| TCAAATACTTGTCCAATCTT+AGG | + | chr6_2:31273965-31273984 | None:intergenic | 30.0% | |
| GTATTTGATACGTTACAAGA+AGG | - | chr6_2:31273977-31273996 | Msa0915270:intron | 30.0% | |
| ATAAAAACGACATTAAGCGT+TGG | - | chr6_2:31274068-31274087 | Msa0915270:intron | 30.0% | |
| GTTGTGTTTATCATTCTTCA+TGG | - | chr6_2:31273481-31273500 | Msa0915270:CDS | 30.0% | |
| !! | AACTTGTTTTCAAATTCCTC+AGG | - | chr6_2:31273517-31273536 | Msa0915270:intron | 30.0% |
| ! | TTCTTTTCATGGGATGATAT+TGG | - | chr6_2:31273722-31273741 | Msa0915270:intron | 30.0% |
| ! | TCTTTTCATGGGATGATATT+GGG | - | chr6_2:31273723-31273742 | Msa0915270:intron | 30.0% |
| !!! | TTGGTTTTATTGCTGTTCAA+TGG | - | chr6_2:31273795-31273814 | Msa0915270:intron | 30.0% |
| !! | TTTTATTGCTGTTCAATGGT+GGG | - | chr6_2:31273799-31273818 | Msa0915270:intron | 30.0% |
| TCAAATACTTGTCCAATCTT+AGG | + | chr6_2:31273965-31273984 | None:intergenic | 30.0% | |
| GTATTTGATACGTTACAAGA+AGG | - | chr6_2:31273977-31273996 | Msa0915270:intron | 30.0% | |
| ATAAAAACGACATTAAGCGT+TGG | - | chr6_2:31274068-31274087 | Msa0915270:intron | 30.0% | |
| TTCCCTTCATTTGCATTTCA+TGG | - | chr6_2:31273413-31273432 | Msa0915270:CDS | 35.0% | |
| GTCCATGAAATGCAAATGAA+GGG | + | chr6_2:31273418-31273437 | None:intergenic | 35.0% | |
| TGTCCATGAAATGCAAATGA+AGG | + | chr6_2:31273419-31273438 | None:intergenic | 35.0% | |
| !! | GTTTTATTGCTGTTCAATGG+TGG | - | chr6_2:31273798-31273817 | Msa0915270:intron | 35.0% |
| CAAGATATAAACAACAGCAC+AGG | + | chr6_2:31273871-31273890 | None:intergenic | 35.0% | |
| TATGAATGTGTGCCTAAGAT+TGG | - | chr6_2:31273950-31273969 | Msa0915270:intron | 35.0% | |
| !! | CATTTTGTTTGCTCGAAAGA+AGG | - | chr6_2:31274094-31274113 | Msa0915270:intron | 35.0% |
| TCCAAATAAGACGGATGATA+AGG | - | chr6_2:31274123-31274142 | Msa0915270:intron | 35.0% | |
| ! | TCCTTATCATCCGTCTTATT+TGG | + | chr6_2:31274127-31274146 | None:intergenic | 35.0% |
| TTCCCTTCATTTGCATTTCA+TGG | - | chr6_2:31273413-31273432 | Msa0915270:CDS | 35.0% | |
| GTCCATGAAATGCAAATGAA+GGG | + | chr6_2:31273418-31273437 | None:intergenic | 35.0% | |
| TGTCCATGAAATGCAAATGA+AGG | + | chr6_2:31273419-31273438 | None:intergenic | 35.0% | |
| !! | GTTTTATTGCTGTTCAATGG+TGG | - | chr6_2:31273798-31273817 | Msa0915270:intron | 35.0% |
| CAAGATATAAACAACAGCAC+AGG | + | chr6_2:31273871-31273890 | None:intergenic | 35.0% | |
| TATGAATGTGTGCCTAAGAT+TGG | - | chr6_2:31273950-31273969 | Msa0915270:intron | 35.0% | |
| !! | CATTTTGTTTGCTCGAAAGA+AGG | - | chr6_2:31274094-31274113 | Msa0915270:intron | 35.0% |
| TCCAAATAAGACGGATGATA+AGG | - | chr6_2:31274123-31274142 | Msa0915270:intron | 35.0% | |
| ! | TCCTTATCATCCGTCTTATT+TGG | + | chr6_2:31274127-31274146 | None:intergenic | 35.0% |
| TGCAAATGAAGGGAAGAAGA+TGG | + | chr6_2:31273408-31273427 | None:intergenic | 40.0% | |
| GAAAAATACGCACTCTCTGT+CGG | - | chr6_2:31274013-31274032 | Msa0915270:intron | 40.0% | |
| TGCAAATGAAGGGAAGAAGA+TGG | + | chr6_2:31273408-31273427 | None:intergenic | 40.0% | |
| GAAAAATACGCACTCTCTGT+CGG | - | chr6_2:31274013-31274032 | Msa0915270:intron | 40.0% | |
| !! | TTGCTGTTCAATGGTGGGTT+TGG | - | chr6_2:31273804-31273823 | Msa0915270:intron | 45.0% |
| !! | TCAATGGTGGGTTTGGCTAT+AGG | - | chr6_2:31273811-31273830 | Msa0915270:intron | 45.0% |
| !! | TTGCTGTTCAATGGTGGGTT+TGG | - | chr6_2:31273804-31273823 | Msa0915270:intron | 45.0% |
| !! | TCAATGGTGGGTTTGGCTAT+AGG | - | chr6_2:31273811-31273830 | Msa0915270:intron | 45.0% |
| !!! | ATATTTATTTTGTTAATTTT+AGG | - | chr6_2:31273925-31273944 | Msa0915270:intron | 5.0% |
| !!! | ATATTTATTTTGTTAATTTT+AGG | - | chr6_2:31273925-31273944 | Msa0915270:intron | 5.0% |
| !! | TGGTGGGTTTGGCTATAGGA+AGG | - | chr6_2:31273815-31273834 | Msa0915270:intron | 50.0% |
| !! | TGGTGGGTTTGGCTATAGGA+AGG | - | chr6_2:31273815-31273834 | Msa0915270:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 31273309 | 31274168 | 31273309 | ID=Msa0915270;Name=Msa0915270 |
| chr6_2 | mRNA | 31273309 | 31274168 | 31273309 | ID=Msa0915270-mRNA-1;Parent=Msa0915270;Name=Msa0915270-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|0.5|1|1|2|0|75 |
| chr6_2 | exon | 31274163 | 31274168 | 31274163 | ID=Msa0915270-mRNA-1:exon:5600;Parent=Msa0915270-mRNA-1 |
| chr6_2 | exon | 31273309 | 31273530 | 31273309 | ID=Msa0915270-mRNA-1:exon:5599;Parent=Msa0915270-mRNA-1 |
| chr6_2 | CDS | 31274163 | 31274168 | 31274163 | ID=Msa0915270-mRNA-1:cds;Parent=Msa0915270-mRNA-1 |
| chr6_2 | CDS | 31273309 | 31273530 | 31273309 | ID=Msa0915270-mRNA-1:cds;Parent=Msa0915270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
