AlfalfaGEDB | Msa0916500

M. sativa cultivar zhongmu-4 / Msa0916500

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009889 GO:0010035 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0916500	TKY57836.1	37.643	263	140	6	3	250	8	261	1.08e-50	176

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0916500	sp\|O65508\|NAC76_ARATH	33.544	158	88	5	4	150	10	161	1.03e-17	84.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0916500	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0916500	MtrunA17_Chr4g0050701	29.508	183	91	7	4	172	11	169	1.65e-17	78.2
Msa0916500	MtrunA17_Chr4g0056541	33.758	157	81	6	4	146	7	154	1.93e-17	80.5
Msa0916500	MtrunA17_Chr5g0405211	33.117	154	86	5	4	146	8	155	6.43e-17	79.0
Msa0916500	MtrunA17_Chr1g0183741	34.641	153	82	7	4	146	10	154	3.83e-16	75.9
Msa0916500	MtrunA17_Chr7g0263931	33.750	160	80	7	4	145	11	162	3.98e-16	76.6
Msa0916500	MtrunA17_Chr2g0318101	31.737	167	83	7	4	146	15	174	7.64e-16	75.9
Msa0916500	MtrunA17_Chr8g0345671	35.065	154	79	8	4	145	11	155	9.74e-16	75.9
Msa0916500	MtrunA17_Chr8g0372121	34.194	155	83	6	4	146	6	153	1.15e-15	75.1
Msa0916500	MtrunA17_Chr5g0417791	30.625	160	85	6	4	146	3	153	1.90e-15	73.6
Msa0916500	MtrunA17_Chr4g0047841	35.556	135	74	5	4	132	15	142	2.46e-15	74.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0916500	AT4G36160.1	33.544	158	88	5	4	150	10	161	1.05e-18	84.7
Msa0916500	AT4G36160.3	33.544	158	88	5	4	150	10	161	1.05e-18	84.7
Msa0916500	AT4G36160.2	33.544	158	88	5	4	150	14	165	1.11e-18	84.7
Msa0916500	AT1G71930.2	33.121	157	88	5	4	149	9	159	5.97e-18	82.0
Msa0916500	AT1G71930.1	33.121	157	88	5	4	149	9	159	5.97e-18	82.0
Msa0916500	AT2G24430.2	35.669	157	82	6	2	146	14	163	2.04e-17	80.5
Msa0916500	AT2G24430.1	35.669	157	82	6	2	146	14	163	2.04e-17	80.5
Msa0916500	AT2G18060.3	33.117	154	86	5	4	146	9	156	3.04e-17	80.5
Msa0916500	AT2G18060.1	33.117	154	86	5	4	146	9	156	3.04e-17	80.5
Msa0916500	AT4G28530.1	33.133	166	85	5	4	147	10	171	5.43e-16	76.6

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Find 48 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACACGACAAAGAACAAA+AGG	0.484780	6_2:+37605382	None:intergenic
AAGTTACTCAGAAACCTATG+GGG	0.699471	6_2:-37605264	Msa0916500:CDS
ACATCCAGATCCATCTCTGG+TGG	0.568257	6_2:+37605352	None:intergenic
AGAATGGCATCAAAACTAGT+TGG	0.512078	6_2:-37605550	Msa0916500:CDS
AGTAAAATTCCCTTGGGTGA+CGG	0.539767	6_2:+37605723	None:intergenic
AGTGCTGCTGTTGAGCAACG+CGG	0.701822	6_2:-37605220	Msa0916500:CDS
AGTTACTCAGAAACCTATGG+GGG	0.738835	6_2:-37605263	Msa0916500:CDS
ATAACAAGGAGTTCATCAGA+AGG	0.650392	6_2:+37605869	None:intergenic
ATAGGAAATTCCGCTCAGAA+TGG	0.595921	6_2:-37605566	Msa0916500:CDS
ATGGATCTGGATGTTGAGTT+TGG	0.395501	6_2:-37605343	Msa0916500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCCAACAAAAATAATGAAG+AGG	-	chr6_2:37603649-37603668	Msa0916500:CDS	30.0%
GAAGAGGTTCAAAATTTCAA+TGG	-	chr6_2:37603665-37603684	Msa0916500:CDS	30.0%
ACATTTAGTAGCAAAGATTG+GGG	+	chr6_2:37604459-37604478	None:intergenic	30.0%
TAAAAGCAATAATTGTGCGT+GGG	+	chr6_2:37604823-37604842	None:intergenic	30.0%
AATAATATTCATAGGAGTGG+GGG	+	chr6_2:37604924-37604943	None:intergenic	30.0%
GAATAATATTCATAGGAGTG+GGG	+	chr6_2:37604925-37604944	None:intergenic	30.0%
ATACGAGGAGTTAAATAAGA+GGG	+	chr6_2:37604968-37604987	None:intergenic	30.0%
GATAACAATTACAATTGGAG+AGG	-	chr6_2:37605026-37605045	Msa0916500:intron	30.0%
ATTACAATTGGAGAGGAATA+TGG	-	chr6_2:37605033-37605052	Msa0916500:intron	30.0%
TGTCTACAAAATGAATCGTA+TGG	-	chr6_2:37605167-37605186	Msa0916500:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	37603584	37605924	37603584	ID=Msa0916500;Name=Msa0916500
chr6_2	mRNA	37603584	37605924	37603584	ID=Msa0916500-mRNA-1;Parent=Msa0916500;Name=Msa0916500-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|250
chr6_2	exon	37605514	37605924	37605514	ID=Msa0916500-mRNA-1:exon:6347;Parent=Msa0916500-mRNA-1
chr6_2	exon	37605183	37605407	37605183	ID=Msa0916500-mRNA-1:exon:6346;Parent=Msa0916500-mRNA-1
chr6_2	exon	37603584	37603700	37603584	ID=Msa0916500-mRNA-1:exon:6345;Parent=Msa0916500-mRNA-1
chr6_2	CDS	37605514	37605924	37605514	ID=Msa0916500-mRNA-1:cds;Parent=Msa0916500-mRNA-1
chr6_2	CDS	37605183	37605407	37605183	ID=Msa0916500-mRNA-1:cds;Parent=Msa0916500-mRNA-1
chr6_2	CDS	37603584	37603700	37603584	ID=Msa0916500-mRNA-1:cds;Parent=Msa0916500-mRNA-1

Gene Sequence

>Msa0916500

ATGGAATTGCTTCCACCTGGTGTTGGCTTCATCCCTTCTGATGAACTCCTTGTTATCTACTACCTTGCCAACAAAAATAATGAAGAGGTTCAAAATTTCAATGGTTCTGACTTGATTAAGGAGCTAAACTTGTACCAGTATGACCCATTTGAATTGCGTCTTACTCCGTCCTTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGATGAGGCAATGTAAGATTGGATTTTGGATGCAGAAGGGGGTTGTTCGAGACATTATTGTTGGAACTGATAACGTGTTGGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCGCTCAGAATGGCATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGCTAACAATCCTCAGGCTCCTTTTGTTCTTTGTCGTGTGTTTGACTGCCCACCAGAGATGGATCTGGATGTTGAGTTTGGAGCAGATGTTGATGAAGGTGATGCTACTGGAGATGGCTCTGCTAACAAGCTGAACGAAGTTACTCAGAAACCTATGGGGGCTAGGGATGGGGCTGGTACTAGTGCTGCTGTTGAGCAACGCGGTGCATCAAATTTCACAGATTTTGAAGGCACCCTGCAGGCGAGTTATGCTGCATTTCTTGACGATGTTGTCTTGCACATGGAAGCTATGACCCCAGAGCAACTAATGCTTTCAATTATGGATGACGATTTTCTTGAAATAGCCGATCTTGTGTAA

Protein sequence

>Msa0916500

MELLPPGVGFIPSDELLVIYYLANKNNEEVQNFNGSDLIKELNLYQYDPFELRLTPSFFFRHPREFYCFTAKVDEQMRQCKIGFWMQKGVVRDIIVGTDNVLVGKKSLFMFYIGNSAQNGIKTSWTMYEYTLANNPQAPFVLCRVFDCPPEMDLDVEFGADVDEGDATGDGSANKLNEVTQKPMGARDGAGTSAAVEQRGASNFTDFEGTLQASYAAFLDDVVLHMEAMTPEQLMLSIMDDDFLEIADLV*