AlfalfaGEDB | Msa0919230

M. sativa cultivar zhongmu-4 / Msa0919230

KEGG: -
GO: -
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919230	XP_039691123.1	80.277	867	161	4	1	867	1	857	0.0	1422

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919230	sp\|O65468\|CRK8_ARATH	43.577	615	319	8	275	867	34	642	8.48e-172	517
Msa0919230	sp\|O65468\|CRK8_ARATH	38.710	248	135	7	24	263	33	271	1.29e-40	163

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919230	MtrunA17_Chr6g0474901	80.277	867	161	4	1	867	1	857	0.0	1422
Msa0919230	MtrunA17_Chr6g0475091	81.560	846	150	3	24	867	31	872	0.0	1385
Msa0919230	MtrunA17_Chr6g0474811	76.800	875	184	9	1	867	1	864	0.0	1329
Msa0919230	MtrunA17_Chr6g0475201	75.000	868	213	3	1	867	1	865	0.0	1327
Msa0919230	MtrunA17_Chr6g0474871	76.355	867	194	6	1	867	1	856	0.0	1316
Msa0919230	MtrunA17_Chr6g0475001	77.584	687	144	7	1	684	1	680	0.0	1077
Msa0919230	MtrunA17_Chr6g0475111	60.137	873	322	12	12	867	14	877	0.0	1003
Msa0919230	MtrunA17_Chr8g0347571	58.770	878	328	12	3	867	4	860	0.0	996
Msa0919230	MtrunA17_Chr6g0474861	68.833	600	135	5	275	867	23	577	0.0	800
Msa0919230	MtrunA17_Chr6g0474861	49.801	251	117	5	1	250	1	243	1.16e-62	223

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919230	AT4G05200.1	42.946	645	334	11	274	891	23	660	2.58e-172	516
Msa0919230	AT4G05200.1	37.302	252	143	8	4	250	7	248	8.85e-38	151
Msa0919230	AT4G23160.1	43.577	615	319	8	275	867	40	648	2.77e-172	516
Msa0919230	AT4G23160.1	38.710	248	135	7	24	263	39	277	1.60e-41	163
Msa0919230	AT4G05200.2	42.946	645	334	11	274	891	41	678	3.88e-172	516
Msa0919230	AT4G05200.2	37.302	252	143	8	4	250	25	266	8.79e-38	152
Msa0919230	AT4G23180.1	44.754	610	314	9	275	867	34	637	8.11e-166	499
Msa0919230	AT4G23180.1	39.130	230	130	7	23	250	32	253	4.18e-34	140
Msa0919230	AT4G23150.1	43.800	621	319	11	275	875	23	633	6.27e-163	491
Msa0919230	AT4G23150.1	40.851	235	120	8	24	250	22	245	1.97e-32	135

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Find 163 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGCAGAGCAAGCAGC+AGG	0.533161	6_2:+50717189	Msa0919230:CDS
AAAACCAGAACTTGTATCAA+CGG	0.603547	6_2:-50717097	None:intergenic
AAAGCAGGTTCTTGTGGAGT+TGG	0.473095	6_2:-50720061	None:intergenic
AAAGCGGAAGAAATAGTGTA+CGG	0.611056	6_2:+50717648	Msa0919230:CDS
AAAGCTTTACCTGCATTTCC+TGG	0.397671	6_2:-50718212	None:intergenic
AAAGGGGAAGAAATAGTCGT+AGG	0.639341	6_2:+50718399	Msa0919230:CDS
AAAGTAATCAAGACTCTTGT+TGG	0.356091	6_2:-50719120	None:intergenic
AAAGTGTATGATGGAAAGAT+AGG	0.436671	6_2:+50717363	Msa0919230:CDS
AAAGTGTGTTTCATGCCCAA+AGG	0.604268	6_2:+50717749	Msa0919230:CDS
AAATAGCATCACCAAAACAA+TGG	0.653033	6_2:-50718351	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCAGAACTTGTATCAA+CGG	-	chr6_2:50717100-50717119	None:intergenic	30.0%
GCGTTAATAGACCTATAAAA+AGG	-	chr6_2:50717435-50717454	None:intergenic	30.0%
TGTTTCGAATCATTTGTTTG+AGG	-	chr6_2:50717480-50717499	None:intergenic	30.0%
TCAATGTGTGAAAAATGCAT+CGG	+	chr6_2:50717713-50717732	Msa0919230:CDS	30.0%
TGTACCAAATTATACCCTTT+GGG	-	chr6_2:50717767-50717786	None:intergenic	30.0%
TGATAGTTACAGAAATTCCA+TGG	+	chr6_2:50718069-50718088	Msa0919230:CDS	30.0%
AGAAATTCCATGGTCAAATT+TGG	+	chr6_2:50718079-50718098	Msa0919230:CDS	30.0%
GAAATTCCATGGTCAAATTT+GGG	+	chr6_2:50718080-50718099	Msa0919230:CDS	30.0%
AAATAGCATCACCAAAACAA+TGG	-	chr6_2:50718354-50718373	None:intergenic	30.0%
TCGTATTTGTGTATGAAGAA+AGG	+	chr6_2:50718451-50718470	Msa0919230:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	50716605	50720413	50716605	ID=Msa0919230;Name=Msa0919230
chr6_2	mRNA	50716605	50720413	50716605	ID=Msa0919230-mRNA-1;Parent=Msa0919230;Name=Msa0919230-mRNA-1;_AED=0.09;_eAED=0.09;_QI=86\|0.85\|0.75\|0.87\|1\|1\|8\|0\|895
chr6_2	exon	50716605	50718224	50716605	ID=Msa0919230-mRNA-1:exon:7567;Parent=Msa0919230-mRNA-1
chr6_2	exon	50718318	50718440	50718318	ID=Msa0919230-mRNA-1:exon:7568;Parent=Msa0919230-mRNA-1
chr6_2	exon	50718554	50718675	50718554	ID=Msa0919230-mRNA-1:exon:7569;Parent=Msa0919230-mRNA-1
chr6_2	exon	50718939	50719149	50718939	ID=Msa0919230-mRNA-1:exon:7570;Parent=Msa0919230-mRNA-1
chr6_2	exon	50719264	50719501	50719264	ID=Msa0919230-mRNA-1:exon:7571;Parent=Msa0919230-mRNA-1
chr6_2	exon	50719608	50719761	50719608	ID=Msa0919230-mRNA-1:exon:7572;Parent=Msa0919230-mRNA-1
chr6_2	exon	50719875	50720094	50719875	ID=Msa0919230-mRNA-1:exon:7573;Parent=Msa0919230-mRNA-1
chr6_2	exon	50720328	50720413	50720328	ID=Msa0919230-mRNA-1:exon:7574;Parent=Msa0919230-mRNA-1
chr6_2	five_prime_UTR	50716605	50716690	50716605	ID=Msa0919230-mRNA-1:five_prime_utr;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50716691	50718224	50716691	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50718318	50718440	50718318	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50718554	50718675	50718554	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50718939	50719149	50718939	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50719264	50719501	50719264	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50719608	50719761	50719608	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50719875	50720094	50719875	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1
chr6_2	CDS	50720328	50720413	50720328	ID=Msa0919230-mRNA-1:cds;Parent=Msa0919230-mRNA-1

Gene Sequence

>Msa0919230

ATGGCTTACTCTACAATTCTCCTTTTATCTAACTTTATCCTTGTTAGATTCCTCTTTTGTGCTACTATGGCACAAGAGTCTCCATTATATCTGAATACAAATTGTCCTATCAATGTTACCACACCCAGCAGCAACTTCCAAAGCAATCTCAAAAAACTCTTCTCTTCATTGTCATCAAAAGCCATAGGCAACACCAAATTTTACAACACCACAGTCACCGGTATAAATCCAACGGATTCTGTCTATGGATTGTTCATGTGCAGGGGTGACGTCTCCCATCAAGTCTGTCATGAATGCATAGCTAATGCAACTCGAAAATTGTCCTTAGACTGCCTTTTATCCAAACAAGCTGAAATCTATTACAACGAGTGCATTGTTCGGTACTCTGACAACTATTTCTTTTCCACCGTTGATACAAGTTCTGGTTTTTACATGTGGAACACAGACAACGTCTCAAACGCAGAAAGCTTCATGCATTTATTGTTTTCCACGATGAATAAAACAGCAGAGCAAGCAGCAGGTCCACTTACTGGTGACAAAAAAAAGATGTTTGCCACAAAAGAAGCACGAATTTCTGAATCTCAGACCCTTTACTGTCTAGTTCAGTGCACGCCGGATTTGTTGCCCAGTGAATGCAGAACATGCCTCGGTCATGCTATTAGGGAACTTTTAAAAGTGTATGATGGAAAGATAGGTGGGAGAAATGTATATCCTAGTTGTAATATTAGGTATGAACCTTATCCTTTTTATAGGTCTATTAACGCTCAATCACCAAATGAGTTTGTTCCTCAAACAAATGATTCGAAACAAGATTCTAGATTATCACAAGATCCAATTTATCTTTCTTATAATTGCTCAAGAAACCACACTGCAATCACTAACAAAAACTTTAAATTACTTCTCTCTTATTTGTCTTCCAACGCTGCAAATGGCAAAAAATTCTATACTGTTAATGTTAAAGCGGAAGAAATAGTGTACGGCCTCTTCTTGTGTCGTGGTGACCTTCCTGTTCGCCTCTGTGGTCAATGTGTGAAAAATGCATCGGATCACATATACTCAAAGTGTGTTTCATGCCCAAAGGGTATAATTTGGTACAGCCACTGCATGGTTCGCTATTCATATCAAACATTATTCTCTAACATCGAAACAAGTCCTGTGTATAGAGATATAAATATCACAAAATATTCCATCACAGACCAAAATTTGTTCACTAGTACATTATCGAATCAGTTATCTCAACTTGCCACTGAAGTAGGGGACAATGATGAAAGATATAAGACTAATTCAGTAAAACTGAATGACGTACAAACCTTGTATACTCTTGGACAATGTACGCGAGACTTGTCTAGTGAGGATTGTGCTAATTGTCTAAATGATATGATAGTTACAGAAATTCCATGGTCAAATTTGGGTAGTGTTGGGGGAAGAATTATATATCCTAGCTGCAATCTGAGGTTTGAATTGTTCCAATTTTACATGGACGGCGACAAAGCTCAGCTGCCTGGGAGTCCTTCTCCATTACCAGGAAATGCAGAGAAACAGAAAATTGTCTTCATTGTTGTCCCTACCATTGTTTTGGTGATGCTATTTTCTATTGGCTACTATTGGCTTAAGCAAAGGGGAAGAAATAGTCGTAGGACTATTCTTCGTGAAAATTTTGGTGAAGAAAGTGCCACTTTAGAGCCACTGCAATTCGATTGGATGGTAATTGAAGCAGCAACCAACAACTTTTCTAAGGACAATTACACTGGCAAAGGAGGGTTTGGAGAGGTTTACAAGGGTATCCTTCTTGATGGAAGAGAAGTGGCTATAAAAAGGCTTTCAAAAAGTTCTAAGCAAGGTGTAGAAGAGTTCAAGAATGAAGTTTTATTGATAGCCAAGCTTCAACATAGAAACCTAGTGGCATTTATTGGTTTTTGCCTTGAAGAACAAGAGAAAATACTTATTTATGAATTTGTGCCCAACAAGAGTCTTGATTACTTTCTATTTGATTCTCAACAACAAAAGTTCTTAACTTGGGGTGAACGCTTCAACATCATAGGAGGAATTGTTCGAGGGATTCTTTATTTGCATGATCATTCTAGACTCAAAGTTATACACCGGGATCTCAAACCTAGCAATATTCTATTAGACGAAAATATGATTCCTAAAATTTCAGATTTTGGTTTGGCTCGAATTGTTGAAATAAGTCAAGATGAGGGAAGTACAAATAGAATCGTTGGAACATATGGTTATATGTCTCCGGAATATGCAATGTTTGGACAATTTTCTGAAAAATCCGACATTTATAGTTTTGGAGTTATGCTTTTAGAGATTATTGCTGGAAAGAAGAATATAAGTTCAGTTACTCCCCAACATGTTGCTGATGGCCTATTGTATCATGTATGGAGACGGTGGATGGAGGAAACACCTTTAAGTATACTGGATCCAAATATTAAAGAAGATTATTCTACAACTGAAGTCATTAAGTGCATTCAGATTGGTCTATTATGTGTTCAAAATGACCCGGATGCTAGACCTTCGATCGTGACAGTTGCTTCTTATCTTAGCAGTTATGCAGTCGAATTACCAACTCCACAAGAACCTGCTTTTTTCTTGCATGACTTTGCGAGTACGAGCCACTTTTCAGACTGCGGCGGACAAAACCAGGAGGCAATGAGCACGAGAGAAGTTTTTTGTGACCAATGA

Protein sequence

>Msa0919230

MAYSTILLLSNFILVRFLFCATMAQESPLYLNTNCPINVTTPSSNFQSNLKKLFSSLSSKAIGNTKFYNTTVTGINPTDSVYGLFMCRGDVSHQVCHECIANATRKLSLDCLLSKQAEIYYNECIVRYSDNYFFSTVDTSSGFYMWNTDNVSNAESFMHLLFSTMNKTAEQAAGPLTGDKKKMFATKEARISESQTLYCLVQCTPDLLPSECRTCLGHAIRELLKVYDGKIGGRNVYPSCNIRYEPYPFYRSINAQSPNEFVPQTNDSKQDSRLSQDPIYLSYNCSRNHTAITNKNFKLLLSYLSSNAANGKKFYTVNVKAEEIVYGLFLCRGDLPVRLCGQCVKNASDHIYSKCVSCPKGIIWYSHCMVRYSYQTLFSNIETSPVYRDINITKYSITDQNLFTSTLSNQLSQLATEVGDNDERYKTNSVKLNDVQTLYTLGQCTRDLSSEDCANCLNDMIVTEIPWSNLGSVGGRIIYPSCNLRFELFQFYMDGDKAQLPGSPSPLPGNAEKQKIVFIVVPTIVLVMLFSIGYYWLKQRGRNSRRTILRENFGEESATLEPLQFDWMVIEAATNNFSKDNYTGKGGFGEVYKGILLDGREVAIKRLSKSSKQGVEEFKNEVLLIAKLQHRNLVAFIGFCLEEQEKILIYEFVPNKSLDYFLFDSQQQKFLTWGERFNIIGGIVRGILYLHDHSRLKVIHRDLKPSNILLDENMIPKISDFGLARIVEISQDEGSTNRIVGTYGYMSPEYAMFGQFSEKSDIYSFGVMLLEIIAGKKNISSVTPQHVADGLLYHVWRRWMEETPLSILDPNIKEDYSTTEVIKCIQIGLLCVQNDPDARPSIVTVASYLSSYAVELPTPQEPAFFLHDFASTSHFSDCGGQNQEAMSTREVFCDQ*