AlfalfaGEDB | Msa0919250

M. sativa cultivar zhongmu-4 / Msa0919250

KEGG: -
GO: -
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919250	XP_039691123.1	81.617	903	138	5	40	923	1	894	0.0	1474

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919250	sp\|O65468\|CRK8_ARATH	43.082	636	333	9	297	907	33	664	1.05e-174	525
Msa0919250	sp\|O65468\|CRK8_ARATH	38.235	238	130	7	68	298	39	266	1.09e-38	157

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919250	MtrunA17_Chr6g0474901	81.617	903	138	5	40	923	1	894	0.0	1474
Msa0919250	MtrunA17_Chr6g0475091	81.888	911	142	5	32	922	1	908	0.0	1454
Msa0919250	MtrunA17_Chr6g0474871	78.627	903	164	9	40	923	1	893	0.0	1372
Msa0919250	MtrunA17_Chr6g0474811	77.851	912	163	12	40	923	1	901	0.0	1356
Msa0919250	MtrunA17_Chr6g0475201	71.188	892	224	6	50	923	13	889	0.0	1260
Msa0919250	MtrunA17_Chr6g0475001	78.280	686	123	9	40	705	1	680	0.0	1069
Msa0919250	MtrunA17_Chr8g0347571	58.850	904	331	14	45	923	10	897	0.0	1006
Msa0919250	MtrunA17_Chr6g0475111	57.174	920	340	18	44	923	9	914	0.0	974
Msa0919250	MtrunA17_Chr6g0474861	70.236	635	133	6	300	923	25	614	0.0	833
Msa0919250	MtrunA17_Chr6g0474861	56.299	254	97	7	40	290	1	243	1.35e-73	254

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919250	AT4G23160.1	43.082	636	333	9	297	907	39	670	2.89e-175	525
Msa0919250	AT4G23160.1	38.235	238	130	7	68	298	45	272	1.49e-39	157
Msa0919250	AT4G05200.1	43.873	661	325	15	299	923	25	675	1.17e-171	515
Msa0919250	AT4G05200.1	36.965	257	145	6	37	290	6	248	7.89e-42	164
Msa0919250	AT4G05200.2	43.873	661	325	15	299	923	43	693	1.69e-171	515
Msa0919250	AT4G05200.2	36.965	257	145	6	37	290	24	266	8.08e-42	164
Msa0919250	AT4G23180.1	43.932	651	327	11	297	923	33	669	1.06e-170	512
Msa0919250	AT4G23180.1	39.316	234	127	7	60	290	32	253	7.43e-37	149
Msa0919250	AT4G23140.1	44.197	629	312	10	288	890	27	642	5.62e-167	503
Msa0919250	AT4G23140.1	39.655	232	128	9	62	290	24	246	7.74e-31	130

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Find 161 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGCAGAAGAAGCAGC+AGG	0.606388	6_2:-50831741	Msa0919250:CDS
AAAACTTCACCTGCATTTCC+TGG	0.371405	6_2:+50830724	None:intergenic
AAAGGGGAAGAAAGAGTCAT+AGG	0.402530	6_2:-50830552	Msa0919250:CDS
AAAGTGTCTTTCATGTCCAA+AGG	0.554698	6_2:-50831187	Msa0919250:CDS
AAAGTTCCATACTGTTGAAG+TGG	0.509358	6_2:-50831304	Msa0919250:CDS
AAATCCAGGAATTGTATCAA+TGG	0.514014	6_2:+50831833	None:intergenic
AAATCCGACATTTATAGTTT+TGG	0.258466	6_2:-50829427	Msa0919250:CDS
AACGACAAAAGTTGTTAACT+TGG	0.437515	6_2:-50829812	Msa0919250:CDS
AACGCAGGTTCTTGTGGAGT+TGG	0.489424	6_2:+50829033	None:intergenic
AACTCCAAAACTATAAATGT+CGG	0.562787	6_2:+50829423	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATCAGTTCGATTTACAT+CGG	+	chr6_2:50829026-50829045	None:intergenic	30.0%
AAATCCAGGAATTGTATCAA+TGG	+	chr6_2:50829218-50829237	None:intergenic	30.0%
GAACTTTCATATGTGTATGA+TGG	-	chr6_2:50829472-50829491	Msa0919250:CDS	30.0%
GCATTAAGAGACCTATAAAA+AGG	+	chr6_2:50829553-50829572	None:intergenic	30.0%
CAATTAGAGGAAAGATAAAC+TGG	+	chr6_2:50829643-50829662	None:intergenic	30.0%
TGTTGTCCCTACAATTATTT+CGG	-	chr6_2:50830437-50830456	Msa0919250:intron	30.0%
TAGATTTCTATGTTGAAGCT+TGG	+	chr6_2:50831022-50831041	None:intergenic	30.0%
AACGACAAAAGTTGTTAACT+TGG	-	chr6_2:50831236-50831255	Msa0919250:CDS	30.0%
ACGACAAAAGTTGTTAACTT+GGG	-	chr6_2:50831237-50831256	Msa0919250:CDS	30.0%
TTACACTCACATGATTTAAG+AGG	+	chr6_2:50831719-50831738	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	50828686	50832384	50828686	ID=Msa0919250;Name=Msa0919250
chr6_2	mRNA	50828686	50832384	50828686	ID=Msa0919250-mRNA-1;Parent=Msa0919250;Name=Msa0919250-mRNA-1;_AED=0.11;_eAED=0.11;_QI=3\|1\|1\|1\|0.62\|0.33\|9\|169\|923
chr6_2	exon	50828686	50828726	50828686	ID=Msa0919250-mRNA-1:exon:7599;Parent=Msa0919250-mRNA-1
chr6_2	exon	50828787	50829241	50828787	ID=Msa0919250-mRNA-1:exon:7598;Parent=Msa0919250-mRNA-1
chr6_2	exon	50829342	50829495	50829342	ID=Msa0919250-mRNA-1:exon:7597;Parent=Msa0919250-mRNA-1
chr6_2	exon	50829603	50829840	50829603	ID=Msa0919250-mRNA-1:exon:7596;Parent=Msa0919250-mRNA-1
chr6_2	exon	50829938	50830148	50829938	ID=Msa0919250-mRNA-1:exon:7595;Parent=Msa0919250-mRNA-1
chr6_2	exon	50830296	50830417	50830296	ID=Msa0919250-mRNA-1:exon:7594;Parent=Msa0919250-mRNA-1
chr6_2	exon	50830533	50830655	50830533	ID=Msa0919250-mRNA-1:exon:7593;Parent=Msa0919250-mRNA-1
chr6_2	exon	50830734	50831433	50830734	ID=Msa0919250-mRNA-1:exon:7592;Parent=Msa0919250-mRNA-1
chr6_2	exon	50831485	50832384	50831485	ID=Msa0919250-mRNA-1:exon:7591;Parent=Msa0919250-mRNA-1
chr6_2	five_prime_UTR	50832382	50832384	50832382	ID=Msa0919250-mRNA-1:five_prime_utr;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50831485	50832381	50831485	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50830734	50831433	50830734	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50830533	50830655	50830533	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50830296	50830417	50830296	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50829938	50830148	50829938	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50829603	50829840	50829603	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50829342	50829495	50829342	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	CDS	50828915	50829241	50828915	ID=Msa0919250-mRNA-1:cds;Parent=Msa0919250-mRNA-1
chr6_2	three_prime_UTR	50828787	50828914	50828787	ID=Msa0919250-mRNA-1:three_prime_utr;Parent=Msa0919250-mRNA-1
chr6_2	three_prime_UTR	50828686	50828726	50828686	ID=Msa0919250-mRNA-1:three_prime_utr;Parent=Msa0919250-mRNA-1

Gene Sequence

>Msa0919250

ATGAGGACATTAGCTTTCCGCGTACACATTGCACGTGTTATGACACTTCCATCAGGCCTTAGAAAAATTTCATTCAAAAACTTCATTGCACCTCTGATTGCAAATCAAAAAAATTCCATGGCTTGCGCTAAAATTTTCTTTTTCTGTATCCTTGTTAATTTCCTCAATTTTGCCACTATCAAGGCACAAGACTCTCTTTGTTATCGTGATAGATTTTGTTCCACCAAGGACAATACCACAGCCAACAGCACTTTCCAAATCAATCTCAAGACCCTCCTCTCTACCTTGTCATCAAAATCCATAGGCAACACTGAATTCTACAACACCACAGTCACCGATGTAAATCGAACTGATTCTGTCTATGGATTGTTCATGTGCAGGGGTGACGACATCTCCTCTCAAATCTGTGATGAATGCATAGTTAATGCAACTCAAAAATTGTCCTTAGATTGCCCTTTATCCAAACAAGCTGTGATTTATTACGATGAGTGCATTGTTCGTTACTCCGACAACTATTTCTTTTCCACCATTGATACAATTCCTGGATTTTACATGTGGAACACTGGGAACGTCTCAAACGCAGAAAGATTCAAGCCTTTATTGTTTTCCACCATGAATAAAACTGCAGAAGAAGCAGCAGGTCCACTTACTGGTGACAAAAACAAGAAGTTTGCCACAACAGAAGCACGGATTTCTGAATCTCAGAAGCTTTACTCTCTAGCTCAGTGCACCCCAGACTTGTCGCTTAATGATTGCAGAACATGCCTCGGTCGTGCTATTAATGAACTTTCATATGTGTATGATGGACAACAAGGTGGGAGAAATTTATATCCTAGTTGTAATATTAGGTATGAACTATATCCTTTTTATAGGTCTCTTAATGCTCCTTCACCAAATGATCCAGTTTATCTTTCCTCTAATTGCTCAAGAAACCACAGTACAATCACTAACAAAAACTTCAAATTACTTCTCTCTTATTTGTCCTCCAACGCCACAAATGGCAAAAAGTTCCATACTGTTGAAGTGGAAGAAATGCTGTATGGCCTCTTCATGTGTCGAGGTGACCTTTCCGTTTGCCTCTGTGGTCGATGTGTGAAAAATGCAACCGATCAAATATACTCAAAGTGTCTTTCATGTCCAAAGGGTATAATTTGGTACAGCCACTGCTTGGTTCGCTATTCAGATAAAAATATCTTCTCTAACATCGAAACAAGTCCTATGTATAGAGATATAAATATCACCACATATTCCATCACATACGAAAACTTGTTCACTAGTACATTATCAAATCAGTTATCTCAACTTGCGAATGATACAGGGAACAGTGATGAAAGGTATAAGACTGATTCATTAAAACTGAATGATGTACAAACCTTGTATACTCTTGGACAATGTACGCGAGATTTGTCTAGTCAGGATTGCGCTTCTTGTCTAAATGATGTCATCGTTTCAGCAATTCCATGGTCAAATTTGGGTAGTGTTGGGGGAAGAATTATATATCCTAACTGCAATCTGAGGTTTGAATTGTTCCGATTTTACATGGAGGGCGACGAACCTCGGCTGTCTGGGAGTCCTTCTCCATTACCAGGAAATGCAGAGAAACGGAAAATCGTCTTCATTGTTGTCCCTACAATTATTTCGGTGATGCTATTTTCTATTGGCTACTATTGGCTAAAGAAAAGGGGAAGAAAGAGTCATAGGACTATTCTTCGAGAAAATTTTGGTGAAGAAAGTGCCACTTTAGAGCCATTGCGATTCGACTGGGTGGTAATTGAAGCAGCAACCAACAACTTCTCTAAGGACAATTACATTGGCAAAGGCGGGTTTGGAGAGGTTTACAAGGGTATCCTTCTTGATGGAAGAGAAGTGGCTATAAAAAGGCTTTCAAAAAGTTCTAAGCAAGGTGTAGAAGAGTTCAAGAATGAAGTTTTATTGATAGCCAAGCTTCAACATAGAAATCTAGTGGTATTTATTGGTTTTTGCCTTGAAGAACATGAGAAAATACTTATTTATGAATTTGTGCCCAACAAAAGTCTTGATTTCTTTCTATTTGATTCTAAACGACAAAAGTTGTTAACTTGGGTTGAACGCTTCAATATCATTGGAGGAATTGTTCGAGGAATTCTTTATTTGCATGAACATTCTCGACTCAAAGTTATACATCGCGATCTCAAACCTAGCAATATTTTATTAGACAAAAATATGATTCCTAAAATTTCAGATTTTGGTTTGGCTCGAATTGTGGAAATAAGTCAAGATGAGGGAAGTACAAAGAGAATTGTTGGAACATATGGTTATATGTCTCCGGAATATGCAATGCTTGGACAATTTTCTGAAAAATCCGACATTTATAGTTTTGGAGTTATGCTTTTAGAGATTATTGCCGGAAAGAATAATATAAGTTCATTTACTCCACAACATGTTGCTGATGGCCTCTTAAATCATGTATGGAGACAGTGGATGGAGGAAACACCTTTAAGTATTTTGGACCCAAATATTAAAGAAGATTATTCTACAAATGAAGTCATTAAGTGCATTCAGATTGGTCTATTATGTGTTCAACATGACCCGGATGCTAGACCTTCAATTGTGACAGTTGCTTCTTACCTTAGCAGTTATGCAGTTGAATTACCAACTCCACAAGAACCTGCGTTTTTCTTACATGGTAGAACTCAAGTTCTTGCACAAGAATCAAGTTCTACTCAATCTGCCAATAGTTCTGCACTATTCTCAAACAATCAAATGTCTGCAAGTACTTTTATTCCTCGATAG

Protein sequence

>Msa0919250

MRTLAFRVHIARVMTLPSGLRKISFKNFIAPLIANQKNSMACAKIFFFCILVNFLNFATIKAQDSLCYRDRFCSTKDNTTANSTFQINLKTLLSTLSSKSIGNTEFYNTTVTDVNRTDSVYGLFMCRGDDISSQICDECIVNATQKLSLDCPLSKQAVIYYDECIVRYSDNYFFSTIDTIPGFYMWNTGNVSNAERFKPLLFSTMNKTAEEAAGPLTGDKNKKFATTEARISESQKLYSLAQCTPDLSLNDCRTCLGRAINELSYVYDGQQGGRNLYPSCNIRYELYPFYRSLNAPSPNDPVYLSSNCSRNHSTITNKNFKLLLSYLSSNATNGKKFHTVEVEEMLYGLFMCRGDLSVCLCGRCVKNATDQIYSKCLSCPKGIIWYSHCLVRYSDKNIFSNIETSPMYRDINITTYSITYENLFTSTLSNQLSQLANDTGNSDERYKTDSLKLNDVQTLYTLGQCTRDLSSQDCASCLNDVIVSAIPWSNLGSVGGRIIYPNCNLRFELFRFYMEGDEPRLSGSPSPLPGNAEKRKIVFIVVPTIISVMLFSIGYYWLKKRGRKSHRTILRENFGEESATLEPLRFDWVVIEAATNNFSKDNYIGKGGFGEVYKGILLDGREVAIKRLSKSSKQGVEEFKNEVLLIAKLQHRNLVVFIGFCLEEHEKILIYEFVPNKSLDFFLFDSKRQKLLTWVERFNIIGGIVRGILYLHEHSRLKVIHRDLKPSNILLDKNMIPKISDFGLARIVEISQDEGSTKRIVGTYGYMSPEYAMLGQFSEKSDIYSFGVMLLEIIAGKNNISSFTPQHVADGLLNHVWRQWMEETPLSILDPNIKEDYSTNEVIKCIQIGLLCVQHDPDARPSIVTVASYLSSYAVELPTPQEPAFFLHGRTQVLAQESSSTQSANSSALFSNNQMSASTFIPR*