AlfalfaGEDB | Msa0919270

M. sativa cultivar zhongmu-4 / Msa0919270

KEGG: -
GO: -
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919270	XP_024641490.2	82.048	869	147	7	1	866	1	863	0.0	1434

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919270	sp\|Q9M0X5\|CRK25_ARATH	44.553	615	309	10	275	862	24	633	3.47e-171	515
Msa0919270	sp\|Q9M0X5\|CRK25_ARATH	39.044	251	140	7	4	250	7	248	6.65e-44	173

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919270	MtrunA17_Chr6g0474901	81.178	866	154	4	1	866	1	857	0.0	1434
Msa0919270	MtrunA17_Chr6g0474811	80.345	870	154	8	1	862	1	861	0.0	1398
Msa0919270	MtrunA17_Chr6g0474871	80.600	866	158	5	1	866	1	856	0.0	1395
Msa0919270	MtrunA17_Chr6g0475091	80.667	869	160	4	1	866	9	872	0.0	1389
Msa0919270	MtrunA17_Chr6g0475201	72.402	866	231	6	1	862	1	862	0.0	1247
Msa0919270	MtrunA17_Chr6g0475001	79.708	685	131	6	1	682	1	680	0.0	1109
Msa0919270	MtrunA17_Chr8g0347571	60.620	871	306	14	9	864	11	859	0.0	1006
Msa0919270	MtrunA17_Chr6g0475111	59.101	890	337	14	3	875	7	886	0.0	994
Msa0919270	MtrunA17_Chr6g0474861	70.500	600	124	5	275	866	23	577	0.0	816
Msa0919270	MtrunA17_Chr6g0474861	50.996	251	114	4	1	250	1	243	2.40e-70	244

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919270	AT4G05200.1	44.553	615	309	10	275	862	24	633	3.53e-172	515
Msa0919270	AT4G05200.1	39.044	251	140	7	4	250	7	248	6.76e-45	173
Msa0919270	AT4G05200.2	44.553	615	309	10	275	862	42	651	5.48e-172	515
Msa0919270	AT4G05200.2	39.044	251	140	7	4	250	25	266	7.18e-45	173
Msa0919270	AT4G23160.1	44.175	618	303	11	275	862	40	645	2.92e-171	513
Msa0919270	AT4G23160.1	38.306	248	136	7	23	263	40	277	2.02e-41	162
Msa0919270	AT4G23180.1	44.663	609	312	9	275	864	34	636	4.99e-167	501
Msa0919270	AT4G23180.1	41.379	232	124	8	21	250	32	253	1.52e-37	150
Msa0919270	AT4G23140.1	43.134	619	316	7	275	862	24	637	1.60e-163	493
Msa0919270	AT4G23140.1	40.851	235	120	8	23	250	24	246	2.84e-35	144

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Find 169 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGGAAGCTTAGAAA+GGG	0.502231	6_2:+51028473	None:intergenic
AAAATTCTCTAAGGTAAATG+TGG	0.508580	6_2:-51027944	Msa0919270:CDS
AAAATTTCACCTGTATTTCC+TGG	0.333291	6_2:+51027364	None:intergenic
AAAGACCATCACCAAAACAA+TGG	0.663310	6_2:+51027221	None:intergenic
AAAGTAATCAAGACTCTTGT+TGG	0.356091	6_2:+51026447	None:intergenic
AAAGTGTCTTTCACGTCCAA+AGG	0.515827	6_2:-51027827	Msa0919270:CDS
AAATCAGACATTTATAGTTT+TGG	0.245769	6_2:-51025915	Msa0919270:CDS
AAATTCTCTAAGGTAAATGT+GGG	0.614195	6_2:-51027943	Msa0919270:CDS
AACAACAAAGGTTGTTAACT+TGG	0.409973	6_2:-51026304	Msa0919270:CDS
AAGGACAATTACATTGGCAA+AGG	0.582936	6_2:-51026937	Msa0919270:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAGTCTAATTGAAAGACA+GGG	+	chr6_2:51025187-51025206	None:intergenic	30.0%
ATTGAGTCTAATTGAAAGAC+AGG	+	chr6_2:51025188-51025207	None:intergenic	30.0%
CAAAACAAAGGAATCGAATA+AGG	+	chr6_2:51025252-51025271	None:intergenic	30.0%
AAAAGAAGGAAGCTTAGAAA+GGG	+	chr6_2:51025626-51025645	None:intergenic	30.0%
TAAAAGAAGGAAGCTTAGAA+AGG	+	chr6_2:51025627-51025646	None:intergenic	30.0%
TCAATGTGTGAAAAATGCAA+CGG	-	chr6_2:51026233-51026252	Msa0919270:CDS	30.0%
AAAATTTCACCTGTATTTCC+TGG	+	chr6_2:51026735-51026754	None:intergenic	30.0%
ATTGTCCTTAGAAAAGTTGT+TGG	+	chr6_2:51027148-51027167	None:intergenic	30.0%
CTTAGTGCTTAGACATTATA+TGG	-	chr6_2:51027224-51027243	Msa0919270:CDS	30.0%
AAAAACCAATAAATGCCACT+AGG	+	chr6_2:51027587-51027606	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	51025138	51028980	51025138	ID=Msa0919270;Name=Msa0919270
chr6_2	mRNA	51025138	51028980	51025138	ID=Msa0919270-mRNA-1;Parent=Msa0919270;Name=Msa0919270-mRNA-1;_AED=0.08;_eAED=0.08;_QI=79\|1\|1\|1\|0.71\|0.62\|8\|133\|887
chr6_2	exon	51025138	51025348	51025138	ID=Msa0919270-mRNA-1:exon:7623;Parent=Msa0919270-mRNA-1
chr6_2	exon	51025374	51025583	51025374	ID=Msa0919270-mRNA-1:exon:7622;Parent=Msa0919270-mRNA-1
chr6_2	exon	51025830	51025983	51025830	ID=Msa0919270-mRNA-1:exon:7621;Parent=Msa0919270-mRNA-1
chr6_2	exon	51026095	51026332	51026095	ID=Msa0919270-mRNA-1:exon:7620;Parent=Msa0919270-mRNA-1
chr6_2	exon	51026440	51026650	51026440	ID=Msa0919270-mRNA-1:exon:7619;Parent=Msa0919270-mRNA-1
chr6_2	exon	51026915	51027036	51026915	ID=Msa0919270-mRNA-1:exon:7618;Parent=Msa0919270-mRNA-1
chr6_2	exon	51027154	51027276	51027154	ID=Msa0919270-mRNA-1:exon:7617;Parent=Msa0919270-mRNA-1
chr6_2	exon	51027374	51028980	51027374	ID=Msa0919270-mRNA-1:exon:7616;Parent=Msa0919270-mRNA-1
chr6_2	five_prime_UTR	51028902	51028980	51028902	ID=Msa0919270-mRNA-1:five_prime_utr;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51027374	51028901	51027374	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51027154	51027276	51027154	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51026915	51027036	51026915	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51026440	51026650	51026440	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51026095	51026332	51026095	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51025830	51025983	51025830	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51025374	51025583	51025374	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	CDS	51025271	51025348	51025271	ID=Msa0919270-mRNA-1:cds;Parent=Msa0919270-mRNA-1
chr6_2	three_prime_UTR	51025138	51025270	51025138	ID=Msa0919270-mRNA-1:three_prime_utr;Parent=Msa0919270-mRNA-1

Gene Sequence

>Msa0919270

ATGACTTACTCCAAAATTATCTTCTTCTCAATCCTTATTCGATTCCTTTGTTTTGCAACTATCAAGGCACAGGACGATCCTATTGTGTCTCGCTACTGTCCCTCCAATGGTACCACAACCAATAGCACCTTCCAAACCAATCTCAAAACCCTCCTCTCTACCTTGTCATCTACAGCCATTGGCAACACTGAATTCTACAACACCACAGTCACTGGTATAAATCCCTCTGATTCTGTCTATGGATTGTTCATGTGCAGGGGTGACGTTTACTCTCAACTCTGTCATAATTGCGTAGTTAATGCAATTCAACAACTCTCCATAGAGTGCCCTTTATCCAAAAAAGCTATTATTTGGTACGACAATTGCATTTGCATGGTTCGATACTCCAACGAGTCTTTCTTTTCCACCCTTTCTAAGCTTCCTTCTTTTATTTTGATGAACACATTAAATGTCTCAAAACCGAATAGCTTTATGCATTTATTGTCATCGACGATGAATCAAACTGCACATGAAGCAGCACGTCCATTTATTGGAGACATAAACAAGAAGTATGCCACCAGGAATGCATCTGTTTTAAATTTTCAGACCCTTTACTGTCTAGCTCAGTGCACGCCGGACTTGTCGCCCAGTGACTGCAGAACCTGTCTCCGTAGTGCTATTGAGGAACTTCTACACTATCATGATGGAAAGAAGGGTGGGAGAATTCTTTTGCCTAGCTGTAATGTTAGGTATGAACTATATCCTTTTTATAGGTCTAATAATGCTCCTTCACCAAATGAGCTTGTTCCTCAAACAAATGATTCAAAACAAGATTCTAGATTTTCAAAAGATCCAATATATCTTTCCTATAATTGCTCAAGAAACCACACTACAATCACTAGAAAAAATTTCAAATTACTTCTCTCTTATTTGTCTTCGAACACCACAAATGGAAAAAAATTCTCTAAGGTAAATGTGGGAGAAATGGTGTACGGCCTCTTCTTGTGTCGAGGTGACCTTCCCGTTCACCTCTGTGGTCAATGTGTGAAAAATGCAACGGATCAAATATACTCAAAGTGTCTTTCACGTCCAAAGGGTATAATTTGGTACAGCCACTGCTTGGTTCGCTATTCAGATAAAGAAATCGCCTCCAACATCGAAACAAGTCCTGTGTATAGCGATATAAATATAACCAAATATTCCCTCACAAACCAAAACTTGTTCACTAGTGCATTATCGAATCAGTTATCTCAACTTGCGAATGATACAGGGGACAATGATGAAAGATATATGACTAATTCATTAAAACTGAATCATGTACAAACCTTGTATACTCTTGGACAATGTACACGAGATTTGTCTAGTGAGGATTGCGCTTCTTGTCTAAATGATGTGATCGTTACAGCAATTCCATGGTCAAAATTGGGGAGTGTTGGAGGAAGAATTATATATCCTAGCTGCAATCTGAGGTTTGAATTGTTCCAATTTTACATGGACGGCGACAAAGCTCAGCTGCCTGAGAGTCCTTCCCCATTACCAGGAAATACAGAGAAACGGAAAATTATCTTCATTGTTGTCCCTACCATTGTTTTGGTGATGGTCTTTTCGACTGGCTACTATTTGCTTAAGAAAAGAGGAAGAAAAAGTCGCAGGACTATTCTCCGAGAAAATTTTGGTGAAGAAAGTGCAACTTTAGAGCCACTGCAATTCGATTGGGTGGTAATTGAAGCAGCAACCAACAACTTTTCTAAGGACAATTACATTGGCAAAGGCGGGTTTGGAGAGGTTTACAAGGGTATTCTTCTTGATGGACGAGAAGTGGCTATAAAAAGGCTTTCAAAAAGTTCAAATCAAGGTGTAGAAGAGTTTAAGAATGAAGTTATATTGATAGCCAAGCTTCAACATAGAAACCTAGTGGCATTTATTGGTTTTTGCCTTGAAGAACAAGAGAAAATACTTATTTATGAATTTGTGCCCAACAAGAGTCTTGATTACTTTCTATTTGATTCTCAACAACAAAGGTTGTTAACTTGGCTTGAACGCTTCAACATCATAGGAGGAATTGTTCGAGGGATTCTTTATTTGCATGAACATTCTCGACTCAAAGTTATACATCGCGATCTCAAACCTAGCAATATTCTATTAGATCAAAATATGATTCCTAAAATTTCAGATTTTGGTTTGGCTCGAATTGTTGAAATAAGTCAAGATGAGGGAAATACAAATAGAATTGCTGGAACATTTGGTTATATGTCTCCGGAATATGCAATGCTTGGACAATTTTCTGAAAAATCAGACATTTATAGTTTTGGAGTTATGCTGTTAGAGATTATTGCTGGAAAGAAGAATAAAAGTTCATGTACTCCACACCAAGTTGCTTATGACCTCTTGAATTATGTATGGAGACAGTGGATGGATCAAACACCTTTAAGTATTTTGGATCCAAATATTAAAGAAGATTATTCTACAACTGAAGTCATTAAGTGCATTCAGATTGGTCTGTTATGTGTTCAAAATGACCCGGATGCTAGACCTTCAATTGTGACAGTTTCTTCTTACCTTAGCAGTTATGCAGTTGAATTACCAACTCCAAAAGAACCTGCATTTACATTCTTCCACAAGAATCAAGTTCTACTCAATCTGCCAATAGTTCTGCACTATTCTCAAACAAACAAATGTCGGTAA

Protein sequence

>Msa0919270

MTYSKIIFFSILIRFLCFATIKAQDDPIVSRYCPSNGTTTNSTFQTNLKTLLSTLSSTAIGNTEFYNTTVTGINPSDSVYGLFMCRGDVYSQLCHNCVVNAIQQLSIECPLSKKAIIWYDNCICMVRYSNESFFSTLSKLPSFILMNTLNVSKPNSFMHLLSSTMNQTAHEAARPFIGDINKKYATRNASVLNFQTLYCLAQCTPDLSPSDCRTCLRSAIEELLHYHDGKKGGRILLPSCNVRYELYPFYRSNNAPSPNELVPQTNDSKQDSRFSKDPIYLSYNCSRNHTTITRKNFKLLLSYLSSNTTNGKKFSKVNVGEMVYGLFLCRGDLPVHLCGQCVKNATDQIYSKCLSRPKGIIWYSHCLVRYSDKEIASNIETSPVYSDINITKYSLTNQNLFTSALSNQLSQLANDTGDNDERYMTNSLKLNHVQTLYTLGQCTRDLSSEDCASCLNDVIVTAIPWSKLGSVGGRIIYPSCNLRFELFQFYMDGDKAQLPESPSPLPGNTEKRKIIFIVVPTIVLVMVFSTGYYLLKKRGRKSRRTILRENFGEESATLEPLQFDWVVIEAATNNFSKDNYIGKGGFGEVYKGILLDGREVAIKRLSKSSNQGVEEFKNEVILIAKLQHRNLVAFIGFCLEEQEKILIYEFVPNKSLDYFLFDSQQQRLLTWLERFNIIGGIVRGILYLHEHSRLKVIHRDLKPSNILLDQNMIPKISDFGLARIVEISQDEGNTNRIAGTFGYMSPEYAMLGQFSEKSDIYSFGVMLLEIIAGKKNKSSCTPHQVAYDLLNYVWRQWMDQTPLSILDPNIKEDYSTTEVIKCIQIGLLCVQNDPDARPSIVTVSSYLSSYAVELPTPKEPAFTFFHKNQVLLNLPIVLHYSQTNKCR*