AlfalfaGEDB | Msa0919910

M. sativa cultivar zhongmu-4 / Msa0919910

KEGG: -
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919910	XP_039021209.1	51.020	98	48	0	36	133	36	133	4.40e-24	108

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919910	sp\|Q9LSE7\|B561C_ARATH	39.568	139	78	3	12	148	13	147	2.49e-21	96.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919910	MtrunA17_Chr7g0234191	39.062	128	78	0	6	133	55	182	2.07e-22	96.7
Msa0919910	MtrunA17_Chr1g0151541	68.421	57	18	0	155	211	827	883	1.43e-18	86.3
Msa0919910	MtrunA17_Chr1g0191171	57.746	71	21	1	155	225	853	914	4.22e-17	82.0
Msa0919910	MtrunA17_Chr8g0392871	56.897	58	25	0	155	212	903	960	1.60e-15	77.0
Msa0919910	MtrunA17_Chr6g0482711	57.895	57	24	0	155	211	824	880	1.81e-15	77.0
Msa0919910	MtrunA17_Chr2g0294351	41.905	105	52	2	155	250	630	734	2.68e-15	76.3
Msa0919910	MtrunA17_Chr3g0132791	43.011	93	29	1	155	223	819	911	6.44e-15	75.5
Msa0919910	MtrunA17_Chr8g0338731	31.884	138	89	2	1	133	1	138	7.43e-15	72.8
Msa0919910	MtrunA17_Chr5g0427651	57.895	57	24	0	155	211	812	868	7.93e-15	75.1
Msa0919910	MtrunA17_Chr1g0179331	51.667	60	29	0	155	214	721	780	1.18e-14	74.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0919910	AT3G25290.1	39.568	139	78	3	12	148	13	147	2.54e-22	96.3
Msa0919910	AT3G25290.2	39.568	139	78	3	12	148	13	147	2.54e-22	96.3
Msa0919910	AT4G12980.1	39.007	141	78	3	12	148	12	148	1.38e-20	91.3
Msa0919910	AT5G11850.1	62.069	58	22	0	155	212	773	830	3.39e-17	82.4
Msa0919910	AT1G08720.2	57.746	71	21	1	155	225	833	894	1.18e-16	80.9
Msa0919910	AT3G06640.2	41.667	108	54	2	155	253	610	717	1.18e-16	80.9
Msa0919910	AT3G06640.3	41.667	108	54	2	155	253	610	717	1.18e-16	80.9
Msa0919910	AT1G08720.1	57.746	71	21	1	155	225	833	894	1.21e-16	80.9
Msa0919910	AT3G06640.1	48.276	87	34	2	155	239	610	687	1.63e-16	80.5
Msa0919910	AT3G06630.2	48.276	87	34	2	155	239	598	675	3.81e-16	79.3

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Find 73 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGAGAGGATCGTGGT+TGG	0.559997	6_2:+53248041	None:intergenic
AAACGTCTTCGAATTCCAAA+TGG	0.505722	6_2:-53246958	Msa0919910:CDS
AAAGGAGTTGCTGACTGCGG+TGG	0.683265	6_2:-53244857	Msa0919910:CDS
AACAACCTGATGAGTTGGAC+AGG	0.535893	6_2:+53247982	None:intergenic
ACAGTGTGGGTGGGCGTGGA+TGG	0.441039	6_2:-53247211	Msa0919910:CDS
ACATAGCGTCAAGTTCAGCG+TGG	0.695271	6_2:+53247920	None:intergenic
ACCACACCATGGGGTTCGTG+TGG	0.608054	6_2:+53247023	None:intergenic
ACCACACGAACCCCATGGTG+TGG	0.623116	6_2:-53247024	Msa0919910:CDS
ACCCAGCCATCAGGCGTTGG+CGG	0.511019	6_2:+53247846	None:intergenic
ACGGTACATACCACACCATG+GGG	0.725550	6_2:+53247014	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATAAAAATAGAGAGGATCG+TGG	+	chr6_2:53244806-53244825	None:intergenic	30.0%
GCCATAGTATAAAAATAGAG+AGG	+	chr6_2:53244813-53244832	None:intergenic	30.0%
ATATTACCTTTACAACATCC+AGG	+	chr6_2:53245198-53245217	None:intergenic	30.0%
ATATCAAATCAATTGAGTGC+TGG	-	chr6_2:53245276-53245295	Msa0919910:intron	30.0%
TCTACATTTATTTCCACTTC+TGG	-	chr6_2:53245329-53245348	Msa0919910:intron	30.0%
AGAAGTTAACGAAAGAAAAG+TGG	-	chr6_2:53245352-53245371	Msa0919910:intron	30.0%
AAAGCCAGATATAAGAGTTT+AGG	-	chr6_2:53245721-53245740	Msa0919910:intron	30.0%
ACATCCTAAACTCTTATATC+TGG	+	chr6_2:53245728-53245747	None:intergenic	30.0%
GAATTCGAAGACGTTTATTT+CGG	+	chr6_2:53245878-53245897	None:intergenic	30.0%
TAATGAAAGGGTAACAAACA+TGG	+	chr6_2:53246020-53246039	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	53244787	53248075	53244787	ID=Msa0919910;Name=Msa0919910
chr6_2	mRNA	53244787	53248075	53244787	ID=Msa0919910-mRNA-1;Parent=Msa0919910;Name=Msa0919910-mRNA-1;_AED=0.50;_eAED=0.51;_QI=0\|0\|0\|0.4\|1\|1\|5\|0\|304
chr6_2	exon	53247652	53248075	53247652	ID=Msa0919910-mRNA-1:exon:7956;Parent=Msa0919910-mRNA-1
chr6_2	exon	53247174	53247255	53247174	ID=Msa0919910-mRNA-1:exon:7955;Parent=Msa0919910-mRNA-1
chr6_2	exon	53246957	53247089	53246957	ID=Msa0919910-mRNA-1:exon:7954;Parent=Msa0919910-mRNA-1
chr6_2	exon	53246164	53246305	53246164	ID=Msa0919910-mRNA-1:exon:7953;Parent=Msa0919910-mRNA-1
chr6_2	exon	53244787	53244920	53244787	ID=Msa0919910-mRNA-1:exon:7952;Parent=Msa0919910-mRNA-1
chr6_2	CDS	53247652	53248075	53247652	ID=Msa0919910-mRNA-1:cds;Parent=Msa0919910-mRNA-1
chr6_2	CDS	53247174	53247255	53247174	ID=Msa0919910-mRNA-1:cds;Parent=Msa0919910-mRNA-1
chr6_2	CDS	53246957	53247089	53246957	ID=Msa0919910-mRNA-1:cds;Parent=Msa0919910-mRNA-1
chr6_2	CDS	53246164	53246305	53246164	ID=Msa0919910-mRNA-1:cds;Parent=Msa0919910-mRNA-1
chr6_2	CDS	53244787	53244920	53244787	ID=Msa0919910-mRNA-1:cds;Parent=Msa0919910-mRNA-1

Gene Sequence

>Msa0919910

ATGACTTCCTTGCCAACCACGATCCTCTCTATTTTTATACTATGGCTGTTGTACATACAACCTGTCTTCGGCCTGTCCAACTCATCAGGTTGTTTTGACCAAATAAAAGGGTACGCTCCCTATCTGCCTTTGCCCACGCTGAACTTGACGCTATGTTACGCGTATGACATACTAAATTCTAGCCTCTCTCTACTTTTCCAAGCGCCCCCGCCAACGCCTGATGGCTGGGTCGCGTGGGGTATAAACCCCACATGCGAAGGCATCTCGGGGGCAGAATTCCTTGCTTCTTTTAAAATGGATAACATGATTGTTGTTACGACATTTTACATTGAATCGAATACTAAAGTTGTTCTTCGACCATTGTCTTTTGAGGTCTGGAATTTAAAAGCTGAAGAGGATGTGGCTAGACCTGGATGTTGTAAAGGACGTAAACGTATATTTTCCTTACAGTGTGGGTGGGCGTGGATGGCACCAAAAGTCTTAAGGAACGAGAGTTGCAATGAAAAGTCCGATGTATATAGTTTCGGTGTGATCTTGTGGGAGTTAACTACCACACGAACCCCATGGTGTGGTATGTACCGTGAGCAGTTTATCAACTATGTTTCGATCCGAAATAAACGTCTTCGAATTCCAAATGGATTTGTCATTGAAAGCCTAACCAAGCTTCTTGACCCGAACTTGCTTTCTCTAACAAACTTGCATCCCGATGTGAGTTTAATTTGGCAGCTGCAATTCCGGCGAAGAAGATGTAATTTCAGCAGAGAAGTGGTGATTTCAGGAGGTTTTGTGGCTCTTAACTGTGATGGAGTTGTGGCAATCGACAAAGGAGTTGCTGACTGCGGTGGTGTCATATGCGATAGTGAAGGTAATTTCAAGGCTGTCTTTCCTTCTACATTAGGCTCATTCAGGTTGTGA

Protein sequence

>Msa0919910

MTSLPTTILSIFILWLLYIQPVFGLSNSSGCFDQIKGYAPYLPLPTLNLTLCYAYDILNSSLSLLFQAPPPTPDGWVAWGINPTCEGISGAEFLASFKMDNMIVVTTFYIESNTKVVLRPLSFEVWNLKAEEDVARPGCCKGRKRIFSLQCGWAWMAPKVLRNESCNEKSDVYSFGVILWELTTTRTPWCGMYREQFINYVSIRNKRLRIPNGFVIESLTKLLDPNLLSLTNLHPDVSLIWQLQFRRRRCNFSREVVISGGFVALNCDGVVAIDKGVADCGGVICDSEGNFKAVFPSTLGSFRL*