AlfalfaGEDB | Msa0922900

M. sativa cultivar zhongmu-4 / Msa0922900

KEGG: K09260|K12412
GO: -
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0922900	XP_013455733.1	71.324	272	36	6	1	268	1	234	6.37e-115	340

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0922900	sp\|O64703\|AGL29_ARATH	31.646	158	99	5	9	159	2	157	3.83e-15	74.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0922900	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0922900	MtrunA17_Chr4g0025531	71.324	272	36	6	1	268	1	234	5.86e-119	340
Msa0922900	MtrunA17_Chr3g0135891	46.541	159	52	5	106	260	16	145	2.07e-32	116
Msa0922900	MtrunA17_Chr3g0099421	35.338	133	76	3	5	127	30	162	2.86e-16	76.3
Msa0922900	MtrunA17_Chr1g0208561	29.560	159	98	3	5	149	5	163	3.45e-16	75.5
Msa0922900	MtrunA17_Chr8g0355091	34.307	137	80	3	1	127	1	137	3.98e-16	75.5
Msa0922900	MtrunA17_Chr3g0089141	29.448	163	101	3	1	149	1	163	6.97e-16	74.7
Msa0922900	MtrunA17_Chr1g0206991	34.559	136	79	3	1	126	1	136	7.85e-16	73.6
Msa0922900	MtrunA17_Chr1g0206701	35.338	133	76	3	5	127	5	137	7.94e-16	74.7
Msa0922900	MtrunA17_Chr1g0208581	32.117	137	83	2	1	127	1	137	1.39e-15	73.9
Msa0922900	MtrunA17_Chr1g0149391	45.588	68	37	0	5	72	5	72	2.08e-15	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0922900	AT4G36590.1	32.353	170	98	5	5	159	3	170	3.00e-17	79.3
Msa0922900	AT2G34440.1	31.646	158	99	5	9	159	2	157	3.90e-16	74.7
Msa0922900	AT1G29962.1	43.750	80	44	1	1	80	1	79	1.47e-15	73.2
Msa0922900	AT5G60440.1	42.308	78	44	1	5	82	3	79	4.69e-14	71.2
Msa0922900	AT2G24840.1	40.000	80	48	0	7	86	61	140	7.14e-14	70.1
Msa0922900	AT1G28460.1	29.605	152	88	3	1	137	1	148	5.90e-13	66.2
Msa0922900	AT1G01530.1	33.645	107	67	1	5	107	3	109	1.59e-12	66.2
Msa0922900	AT3G04100.1	35.714	84	51	1	5	88	10	90	1.33e-11	62.8
Msa0922900	AT3G66656.1	40.000	65	39	0	9	73	2	66	3.57e-11	61.2
Msa0922900	AT1G28450.1	38.750	80	48	1	1	80	1	79	6.38e-11	60.5

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Find 34 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCTGGAATTTACAACA+AGG	0.757794	6_2:-62617520	Msa0922900:CDS
AACAGCAAATATCATGAAAT+TGG	0.280810	6_2:-62616923	Msa0922900:CDS
AACCTTATTGGTGCAAGCGG+AGG	0.596051	6_2:-62616567	Msa0922900:CDS
AAGATGTACACATACCACGA+GGG	0.757349	6_2:+62617112	None:intergenic
AAGCATGAGAATATCAACTG+AGG	0.702546	6_2:+62617470	None:intergenic
ACACAAGGAGTCAACCTTAT+TGG	0.323352	6_2:-62616579	Msa0922900:CDS
ATCATTTCCTCTTCTATTGA+AGG	0.331143	6_2:+62617369	None:intergenic
ATCCTCCGCTTGCACCAATA+AGG	0.357163	6_2:+62616565	None:intergenic
ATTCTCATGCTTTCTCCAAC+GGG	0.403336	6_2:-62617459	Msa0922900:CDS
CCGCCAAAGAGGTCATCAAG+AGG	0.664394	6_2:-62617609	Msa0922900:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAAGAGGAAAACAAAAAG+TGG	-	chr6_2:62616544-62616563	Msa0922900:CDS	30.0%
TGAAAATCCTTCAATAGAAG+AGG	-	chr6_2:62616763-62616782	Msa0922900:intron	30.0%
ATCATTTCCTCTTCTATTGA+AGG	+	chr6_2:62616773-62616792	None:intergenic	30.0%
TTTCTTTCACTTGATCATAC+TGG	+	chr6_2:62616937-62616956	None:intergenic	30.0%
TTTATTTGTGACAGCAACTA+AGG	+	chr6_2:62616969-62616988	None:intergenic	30.0%
TATGTATTGTCTTCCTTTCA+TGG	-	chr6_2:62617476-62617495	Msa0922900:CDS	30.0%
ATCAAGAGGAAAACAAAAAG+TGG	-	chr6_2:62616544-62616563	Msa0922900:CDS	30.0%
TGAAAATCCTTCAATAGAAG+AGG	-	chr6_2:62616763-62616782	Msa0922900:intron	30.0%
ATCATTTCCTCTTCTATTGA+AGG	+	chr6_2:62616773-62616792	None:intergenic	30.0%
TTTCTTTCACTTGATCATAC+TGG	+	chr6_2:62616937-62616956	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	62616527	62617634	62616527	ID=Msa0922900;Name=Msa0922900
chr6_2	mRNA	62616527	62617634	62616527	ID=Msa0922900-mRNA-1;Parent=Msa0922900;Name=Msa0922900-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|0\|0\|1\|1\|1\|3\|0\|268
chr6_2	exon	62617127	62617634	62617127	ID=Msa0922900-mRNA-1:exon:9705;Parent=Msa0922900-mRNA-1
chr6_2	exon	62616918	62617007	62616918	ID=Msa0922900-mRNA-1:exon:9704;Parent=Msa0922900-mRNA-1
chr6_2	exon	62616527	62616735	62616527	ID=Msa0922900-mRNA-1:exon:9703;Parent=Msa0922900-mRNA-1
chr6_2	CDS	62617127	62617634	62617127	ID=Msa0922900-mRNA-1:cds;Parent=Msa0922900-mRNA-1
chr6_2	CDS	62616918	62617007	62616918	ID=Msa0922900-mRNA-1:cds;Parent=Msa0922900-mRNA-1
chr6_2	CDS	62616527	62616735	62616527	ID=Msa0922900-mRNA-1:cds;Parent=Msa0922900-mRNA-1

Gene Sequence

>Msa0922900

ATGCCGCCAAAGAGGTCATCAAGAGGAAAACAAAAAGTGGAGCTAAAAAAAATTGAGAGTAAGAATGATCTCTTTGTTGCATTTACAACGAGAAAGTCTGGAATTTACAACAAGGCCTCTGAGTTAACAACACTTTCAGGAGCCTCAGTTGATATTCTCATGCTTTCTCCAACGGGAAAACCATTTTGTTATGGAAAACCATCTTCTGAATCTATAACAAAAACTAGTTTGAATGATGAAAATCCTTCAATAGAAGAGGAAATGATGAAACCGAATATTATACAGCAAAATGAAAAAAATGATGATCTTTTGGATGAAAAGCATGTTGCGGAAGCACAACGTAAAAATTTGAAGATGTTGGAAAGTTCTGGATATTGGTCAATTCAAGAGAAAGATGATAAAAGCTACCAGTATGATCAAGTGAAAGAAATTGACAAATCCTTAGTTGCTGTCACAAATAAATTGATAAATAAAGTCTTGAAAAATGGAGGAGATCTTAACCCTCGTGTAATTTCTACCATCGAGGAACTGAATGGAGTCAAAACAAATCCTGCTTATGTTTTTGAGGACAACAGCAAATATCATGAAATTGGTGCAAGCATGCAAACATTTACTTATAATATTTCATGCGACAAATCTAACATATGTTATGTATTGTCTTCCTTTCATGGATTCAATCTTTTTGATCCAAAAGGTAAAGGTATGGTTGCTAATGCTTCTCGTGATATAGAAACACAAGGAGTCAACCTTATTGGTGCAAGCGGAGGATATGAAAGTGGTGTTGATCATGCTTCTCGAGATATATGA

Protein sequence

>Msa0922900

MPPKRSSRGKQKVELKKIESKNDLFVAFTTRKSGIYNKASELTTLSGASVDILMLSPTGKPFCYGKPSSESITKTSLNDENPSIEEEMMKPNIIQQNEKNDDLLDEKHVAEAQRKNLKMLESSGYWSIQEKDDKSYQYDQVKEIDKSLVAVTNKLINKVLKNGGDLNPRVISTIEELNGVKTNPAYVFEDNSKYHEIGASMQTFTYNISCDKSNICYVLSSFHGFNLFDPKGKGMVANASRDIETQGVNLIGASGGYESGVDHASRDI*