AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0924170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924170 MtrunA17_Chr7g0214071 84.921 126 17 2 30 153 59 184 4.40e-72 218
Msa0924170 MtrunA17_Chr7g0243371 83.333 84 14 0 35 118 16 99 9.02e-48 152
Msa0924170 MtrunA17_Chr5g0405211 48.305 118 58 2 28 144 56 171 6.20e-33 120
Msa0924170 MtrunA17_Chr1g0200561 54.783 115 49 3 27 140 66 178 6.70e-33 120
Msa0924170 MtrunA17_Chr1g0147421 52.893 121 47 2 34 153 79 190 1.12e-32 120
Msa0924170 MtrunA17_Chr4g0017921 58.763 97 36 3 36 131 64 157 3.62e-32 118
Msa0924170 MtrunA17_Chr4g0018391 56.701 97 39 2 36 131 63 157 1.01e-31 117
Msa0924170 MtrunA17_Chr3g0139331 52.885 104 48 1 34 136 62 165 2.39e-31 116
Msa0924170 MtrunA17_Chr2g0309331 55.670 97 41 2 36 131 66 161 7.14e-31 115
Msa0924170 MtrunA17_Chr7g0261411 52.941 102 44 2 30 128 54 154 2.81e-30 112
Msa0924170 MtrunA17_Chr1g0183741 52.885 104 45 2 30 130 54 156 3.95e-30 111
Msa0924170 MtrunA17_Chr4g0042561 53.774 106 40 3 36 134 62 165 5.56e-30 111
Msa0924170 MtrunA17_Chr2g0309891 51.304 115 48 4 38 146 63 175 6.63e-30 112
Msa0924170 MtrunA17_Chr4g0060931 50.435 115 47 4 30 136 61 173 8.12e-30 112
Msa0924170 MtrunA17_Chr8g0372121 53.608 97 42 2 36 131 62 156 9.05e-30 111
Msa0924170 MtrunA17_Chr3g0109821 55.752 113 43 5 38 145 63 173 1.12e-29 114
Msa0924170 MtrunA17_Chr6g0461661 50.980 102 45 1 34 130 69 170 1.75e-29 112
Msa0924170 MtrunA17_Chr8g0385991 50.450 111 47 2 36 138 65 175 2.18e-29 108
Msa0924170 MtrunA17_Chr8g0346001 50.926 108 39 2 36 131 71 176 3.49e-29 112
Msa0924170 MtrunA17_Chr4g0047841 47.934 121 37 2 36 130 68 188 3.82e-29 110
Msa0924170 MtrunA17_Chr5g0398971 53.061 98 43 2 36 131 63 159 4.26e-29 110
Msa0924170 MtrunA17_Chr2g0316971 50.943 106 47 1 34 134 74 179 4.37e-29 110
Msa0924170 MtrunA17_Chr4g0056541 54.639 97 41 2 36 131 63 157 8.86e-29 109
Msa0924170 MtrunA17_Chr3g0131911 50.877 114 45 2 36 138 65 178 1.58e-28 106
Msa0924170 MtrunA17_Chr7g0252971 46.032 126 50 4 36 144 61 185 2.22e-28 108
Msa0924170 MtrunA17_Chr6g0484441 53.061 98 43 2 34 130 56 151 2.24e-28 108
Msa0924170 MtrunA17_Chr2g0318101 49.541 109 41 2 36 130 68 176 2.95e-28 108
Msa0924170 MtrunA17_Chr5g0417791 51.456 103 41 2 36 131 56 156 3.34e-28 106
Msa0924170 MtrunA17_Chr2g0318081 52.885 104 44 2 36 136 69 170 7.74e-28 107
Msa0924170 MtrunA17_Chr3g0124761 45.714 140 64 4 1 131 22 158 1.00e-27 105
Msa0924170 MtrunA17_Chr1g0197361 48.571 105 50 2 38 140 66 168 1.22e-27 108
Msa0924170 MtrunA17_Chr3g0144271 39.869 153 64 3 30 182 71 195 1.22e-27 107
Msa0924170 MtrunA17_Chr3g0091921 49.074 108 48 2 34 136 67 172 1.28e-27 105
Msa0924170 MtrunA17_Chr3g0113001 49.107 112 49 3 31 136 13 122 1.47e-27 105
Msa0924170 MtrunA17_Chr2g0312321 49.000 100 45 2 37 132 63 160 2.39e-27 105
Msa0924170 MtrunA17_Chr8g0384721 44.928 138 63 4 4 131 25 159 2.83e-27 104
Msa0924170 MtrunA17_Chr7g0263931 50.505 99 42 2 36 127 64 162 3.62e-27 104
Msa0924170 MtrunA17_Chr6g0481091 49.057 106 48 4 38 140 63 165 3.75e-27 102
Msa0924170 MtrunA17_Chr5g0433781 55.208 96 38 3 38 130 63 156 3.78e-27 107
Msa0924170 MtrunA17_Chr3g0130731 45.255 137 63 4 4 131 25 158 5.80e-27 103
Msa0924170 MtrunA17_Chr6g0453451 46.087 115 52 3 30 136 64 176 9.27e-27 103
Msa0924170 MtrunA17_Chr5g0428991 49.533 107 44 3 28 131 77 176 2.01e-26 105
Msa0924170 MtrunA17_Chr7g0217721 42.400 125 52 2 30 136 66 188 2.26e-26 103
Msa0924170 MtrunA17_Chr7g0253011 51.923 104 43 3 34 130 68 171 2.50e-26 103
Msa0924170 MtrunA17_Chr2g0326361 48.485 99 44 1 36 127 67 165 3.00e-26 102
Msa0924170 MtrunA17_Chr8g0359921 51.887 106 44 4 36 136 67 170 4.09e-26 102
Msa0924170 MtrunA17_Chr3g0092671 50.000 104 48 2 38 139 63 164 6.45e-26 103
Msa0924170 MtrunA17_Chr8g0364791 46.226 106 52 2 38 140 63 166 1.84e-25 101
Msa0924170 MtrunA17_Chr5g0417001 42.857 112 53 1 36 136 67 178 1.42e-24 99.0
Msa0924170 MtrunA17_Chr8g0361731 41.593 113 54 1 36 136 67 179 1.43e-24 99.0
Msa0924170 MtrunA17_Chr3g0128151 50.000 106 43 3 28 130 74 172 1.75e-24 99.8
Msa0924170 MtrunA17_Chr7g0217711 53.086 81 36 1 30 110 66 144 1.94e-24 94.4
Msa0924170 MtrunA17_Chr3g0128111 48.958 96 44 2 38 130 99 192 2.18e-24 95.9
Msa0924170 MtrunA17_Chr5g0400461 40.833 120 66 1 38 152 63 182 2.54e-24 97.4
Msa0924170 MtrunA17_Chr3g0113031 49.495 99 35 3 48 136 5 98 1.13e-23 94.4
Msa0924170 MtrunA17_Chr8g0384201 44.340 106 54 2 38 140 63 166 1.52e-23 96.7
Msa0924170 MtrunA17_Chr4g0038751 43.750 96 51 2 36 130 68 161 4.90e-23 93.2
Msa0924170 MtrunA17_Chr1g0201021 43.519 108 56 2 36 140 74 179 7.34e-23 95.1
Msa0924170 MtrunA17_Chr3g0130101 48.077 104 49 3 40 140 66 167 9.01e-23 93.6
Msa0924170 MtrunA17_Chr4g0054791 41.739 115 63 3 38 148 63 177 5.23e-22 90.9
Msa0924170 MtrunA17_Chr4g0076731 45.631 103 52 3 36 137 107 206 1.58e-21 90.9
Msa0924170 MtrunA17_Chr5g0442771 45.283 106 45 3 36 128 71 176 2.45e-21 89.0
Msa0924170 MtrunA17_Chr6g0453131 42.991 107 48 3 36 130 68 173 2.52e-21 90.1
Msa0924170 MtrunA17_Chr2g0311651 40.625 128 56 3 36 147 76 199 2.92e-21 89.0
Msa0924170 MtrunA17_Chr1g0197351 45.614 114 49 3 36 139 97 207 5.87e-21 89.4
Msa0924170 MtrunA17_Chr2g0311661 44.643 112 45 3 36 131 75 185 2.77e-20 85.9
Msa0924170 MtrunA17_Chr2g0321741 46.667 90 42 2 38 127 48 131 4.02e-20 86.3
Msa0924170 MtrunA17_Chr8g0345671 44.444 90 47 2 38 127 69 155 3.07e-17 78.6
Msa0924170 MtrunA17_Chr2g0321631 40.404 99 52 3 37 129 64 161 1.07e-16 75.5
Msa0924170 MtrunA17_Chr4g0026141 42.000 100 51 3 36 129 63 161 3.29e-16 74.3
Msa0924170 MtrunA17_Chr7g0266701 35.577 104 58 3 37 132 64 166 1.03e-15 72.8
Msa0924170 MtrunA17_Chr4g0050701 38.542 96 49 2 55 141 75 169 2.17e-15 70.9
Msa0924170 MtrunA17_Chr8g0345721 43.836 73 40 1 36 108 120 191 5.70e-14 67.4
Msa0924170 MtrunA17_Chr8g0345691 36.264 91 55 2 38 128 70 157 4.28e-13 66.6
Msa0924170 MtrunA17_Chr8g0345741 34.737 95 55 3 36 130 47 134 2.22e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0924170 AT5G13180.1 68.182 110 33 1 30 137 58 167 9.52e-49 159
Msa0924170 AT2G33480.2 65.421 107 34 2 30 135 59 163 7.07e-44 147
Msa0924170 AT2G33480.3 66.019 103 30 2 36 135 8 108 1.06e-43 145
Msa0924170 AT2G33480.1 64.220 109 34 2 30 135 59 165 1.55e-43 146
Msa0924170 AT4G10350.2 48.062 129 48 3 6 131 2 114 2.06e-34 123
Msa0924170 AT1G33280.1 57.292 96 39 1 36 131 64 157 3.93e-34 123
Msa0924170 AT4G10350.1 56.566 99 38 2 36 131 65 161 4.48e-33 121
Msa0924170 AT2G24430.2 56.190 105 44 1 34 136 67 171 5.15e-33 120
Msa0924170 AT2G24430.1 56.190 105 44 1 34 136 67 171 5.15e-33 120
Msa0924170 AT5G61430.1 53.043 115 44 4 30 136 60 172 9.50e-32 117
Msa0924170 AT5G61430.2 54.630 108 42 3 34 136 4 109 9.66e-32 116
Msa0924170 AT5G07680.2 53.043 115 44 4 30 136 47 159 1.24e-31 116
Msa0924170 AT2G18060.3 52.679 112 49 3 30 139 59 168 1.31e-31 117
Msa0924170 AT2G18060.1 52.679 112 49 3 30 139 59 168 1.31e-31 117
Msa0924170 AT5G07680.1 53.043 115 44 4 30 136 61 173 1.66e-31 116
Msa0924170 AT4G36160.1 51.327 113 50 3 30 139 60 170 6.36e-31 116
Msa0924170 AT4G36160.3 51.327 113 50 3 30 139 60 170 6.36e-31 116
Msa0924170 AT4G36160.2 51.327 113 50 3 30 139 64 174 6.40e-31 116
Msa0924170 AT5G62380.2 56.701 97 39 2 36 131 63 157 7.78e-31 115
Msa0924170 AT5G62380.1 56.701 97 39 2 36 131 63 157 7.78e-31 115
Msa0924170 AT5G66300.1 53.271 107 44 3 28 131 60 163 8.99e-31 114
Msa0924170 AT3G12977.2 54.808 104 41 3 37 136 19 120 1.19e-30 112
Msa0924170 AT1G76420.1 49.091 110 53 2 30 137 69 177 1.39e-30 114
Msa0924170 AT1G71930.2 55.238 105 44 3 27 130 56 158 1.41e-30 114
Msa0924170 AT1G71930.1 55.238 105 44 3 27 130 56 158 1.41e-30 114
Msa0924170 AT1G12260.2 51.961 102 46 2 36 136 63 162 1.92e-30 114
Msa0924170 AT1G62700.1 47.328 131 58 4 36 163 63 185 1.93e-30 115
Msa0924170 AT1G62700.2 47.328 131 58 4 36 163 63 185 1.93e-30 115
Msa0924170 AT5G39610.1 50.926 108 46 2 34 136 71 176 2.81e-30 112
Msa0924170 AT1G77450.1 47.328 131 57 4 30 151 54 181 4.68e-30 111
Msa0924170 AT3G18400.1 54.286 105 44 3 34 136 56 158 4.81e-30 112
Msa0924170 AT1G12260.1 51.961 102 46 2 36 136 63 162 4.81e-30 114
Msa0924170 AT3G12977.1 54.808 104 41 3 37 136 72 173 7.88e-30 111
Msa0924170 AT4G28530.1 53.535 99 45 1 37 135 80 177 8.79e-30 112
Msa0924170 AT1G79580.3 54.639 97 41 2 36 131 73 167 1.02e-29 112
Msa0924170 AT1G79580.5 54.639 97 41 2 36 131 73 167 1.02e-29 112
Msa0924170 AT1G79580.4 54.639 97 41 2 36 131 73 167 1.02e-29 112
Msa0924170 AT1G79580.2 54.639 97 41 2 36 131 73 167 1.02e-29 112
Msa0924170 AT1G79580.1 54.639 97 41 2 36 131 73 167 1.02e-29 112
Msa0924170 AT4G27410.2 47.500 120 58 2 36 152 67 184 1.05e-29 111
Msa0924170 AT4G27410.3 47.500 120 58 2 36 152 84 201 1.42e-29 111
Msa0924170 AT5G18270.2 53.922 102 41 2 34 130 72 172 2.52e-29 111
Msa0924170 AT5G18270.1 53.922 102 41 2 34 130 72 172 2.60e-29 111
Msa0924170 AT3G15170.1 49.020 102 47 1 34 130 71 172 3.40e-29 110
Msa0924170 AT1G69490.1 53.704 108 40 3 36 136 62 166 4.14e-29 108
Msa0924170 AT5G53950.1 52.294 109 44 3 30 130 61 169 1.96e-28 109
Msa0924170 AT3G15510.1 49.541 109 41 2 36 130 70 178 1.99e-28 109
Msa0924170 AT1G65910.1 45.312 128 63 4 27 151 53 176 2.23e-28 111
Msa0924170 AT1G56010.1 48.571 105 46 2 37 135 7 109 2.55e-28 106
Msa0924170 AT2G46770.1 44.361 133 55 3 36 152 72 201 4.16e-28 108
Msa0924170 AT3G04060.1 54.902 102 40 3 34 130 71 171 4.23e-28 107
Msa0924170 AT3G10500.1 50.000 106 48 2 38 140 66 169 1.05e-27 108
Msa0924170 AT1G56010.2 48.571 105 46 2 37 135 74 176 1.12e-27 106
Msa0924170 AT5G04410.1 50.943 106 47 2 38 140 66 169 1.66e-27 108
Msa0924170 AT1G33060.2 52.577 97 41 2 37 130 80 174 2.16e-27 108
Msa0924170 AT1G33060.1 52.577 97 41 2 37 130 80 174 2.28e-27 108
Msa0924170 AT4G35580.1 52.778 108 43 3 38 139 66 171 2.29e-27 108
Msa0924170 AT4G35580.3 52.778 108 43 3 38 139 66 171 2.35e-27 108
Msa0924170 AT3G04070.2 52.778 108 38 3 36 130 63 170 2.46e-27 105
Msa0924170 AT4G35580.2 52.778 108 43 3 38 139 66 171 2.48e-27 108
Msa0924170 AT1G54330.1 56.989 93 38 1 38 130 63 153 2.87e-27 105
Msa0924170 AT1G61110.1 51.818 110 37 3 36 130 69 177 3.44e-27 105
Msa0924170 AT3G61910.1 47.748 111 40 3 36 130 67 175 6.07e-27 104
Msa0924170 AT1G01720.1 42.568 148 72 4 1 138 22 166 6.34e-27 103
Msa0924170 AT5G08790.1 43.885 139 64 4 4 131 25 160 8.12e-27 103
Msa0924170 AT1G32770.1 47.706 109 42 3 36 131 72 178 9.31e-27 104
Msa0924170 AT5G63790.1 46.094 128 55 4 15 131 79 203 9.76e-27 103
Msa0924170 AT5G63790.2 46.094 128 55 4 15 131 89 213 1.21e-26 103
Msa0924170 AT3G17730.1 50.000 96 43 3 38 130 63 156 1.27e-26 102
Msa0924170 AT3G03200.1 49.074 108 48 3 27 130 53 157 1.36e-26 105
Msa0924170 AT1G52890.1 50.962 104 46 2 36 136 67 168 1.61e-26 103
Msa0924170 AT3G15500.1 50.962 104 46 2 36 136 67 168 1.76e-26 103
Msa0924170 AT5G17260.1 44.000 125 63 3 27 147 53 174 2.22e-26 105
Msa0924170 AT3G29035.1 45.600 125 59 3 36 155 77 197 2.96e-26 102
Msa0924170 AT3G10480.3 48.598 107 49 2 36 138 115 219 3.44e-26 104
Msa0924170 AT3G10490.1 47.664 107 50 2 36 138 82 186 4.27e-26 100
Msa0924170 AT3G10480.1 49.057 106 48 2 37 138 83 186 5.42e-26 103
Msa0924170 AT5G46590.1 50.515 97 43 2 38 130 63 158 1.11e-25 100
Msa0924170 AT3G04070.1 52.885 104 38 2 36 136 63 158 2.07e-25 101
Msa0924170 AT3G10480.2 48.113 106 50 2 36 138 82 185 9.07e-25 100
Msa0924170 AT3G10490.2 48.113 106 49 2 37 138 83 186 1.03e-24 100
Msa0924170 AT2G17040.1 47.917 96 47 2 36 130 59 152 4.04e-24 96.3
Msa0924170 AT1G34190.1 45.872 109 52 3 36 140 71 176 7.10e-24 98.2
Msa0924170 AT1G52880.1 45.133 113 49 3 36 135 70 182 1.10e-23 95.9
Msa0924170 AT5G46590.2 50.000 84 41 1 38 121 63 145 1.88e-23 94.4
Msa0924170 AT5G24590.2 43.548 124 55 4 38 151 70 188 1.83e-22 94.0
Msa0924170 AT2G02450.1 44.643 112 55 4 30 137 95 203 1.99e-22 93.2
Msa0924170 AT1G34180.4 43.519 108 56 2 36 140 83 188 2.25e-22 93.6
Msa0924170 AT1G34180.1 43.519 108 56 2 36 140 71 176 2.29e-22 94.0
Msa0924170 AT1G34180.2 43.519 108 56 2 36 140 83 188 2.36e-22 94.0
Msa0924170 AT1G34180.3 43.519 108 56 2 36 140 71 176 2.51e-22 93.6
Msa0924170 AT3G49530.1 45.918 98 49 2 38 132 70 166 2.62e-22 93.6
Msa0924170 AT2G02450.2 47.222 108 51 4 30 133 95 200 3.58e-22 92.8
Msa0924170 AT3G49530.2 45.918 98 49 2 38 132 70 166 3.78e-22 92.8
Msa0924170 AT3G12910.1 44.954 109 44 3 36 128 75 183 5.17e-22 91.3
Msa0924170 AT4G17980.1 53.425 73 34 0 38 110 63 135 7.24e-22 90.1
Msa0924170 AT5G64060.1 42.308 104 55 2 40 140 65 166 8.74e-22 91.3
Msa0924170 AT5G09330.5 45.192 104 52 2 40 140 65 166 1.09e-21 92.0
Msa0924170 AT5G09330.2 45.192 104 52 2 40 140 65 166 1.09e-21 92.0
Msa0924170 AT5G09330.4 45.192 104 52 2 40 140 65 166 1.09e-21 92.0
Msa0924170 AT5G09330.3 45.192 104 52 2 40 140 65 166 1.09e-21 92.0
Msa0924170 AT5G09330.1 45.192 104 52 2 40 140 65 166 1.09e-21 92.0
Msa0924170 AT4G17980.2 53.425 73 34 0 38 110 63 135 1.29e-21 89.7
Msa0924170 AT5G39820.1 39.200 125 52 2 30 130 67 191 1.53e-21 90.5
Msa0924170 AT1G32510.1 41.935 124 63 2 38 152 63 186 4.71e-21 88.2
Msa0924170 AT1G26870.1 42.056 107 50 2 36 130 69 175 7.10e-21 89.4
Msa0924170 AT2G43000.1 47.423 97 45 3 36 128 71 165 1.16e-20 87.0
Msa0924170 AT2G18060.2 51.852 81 35 3 61 139 2 80 2.47e-20 86.3
Msa0924170 AT5G22290.1 40.870 115 60 3 38 152 77 183 3.91e-20 86.7
Msa0924170 AT3G10490.4 48.276 87 39 2 56 138 2 86 3.97e-19 84.0
Msa0924170 AT3G10490.3 48.276 87 39 2 56 138 2 86 3.97e-19 84.0
Msa0924170 AT1G32870.3 41.818 110 55 4 36 140 101 206 2.11e-18 82.8
Msa0924170 AT1G32870.1 41.818 110 55 4 36 140 65 170 2.37e-18 82.4
Msa0924170 AT1G32870.2 41.818 110 55 4 36 140 65 170 2.43e-18 82.4
Msa0924170 AT3G44290.2 39.640 111 58 3 38 148 70 171 4.66e-18 79.3
Msa0924170 AT4G01540.3 50.667 75 36 1 38 111 63 137 5.40e-18 81.3
Msa0924170 AT4G01540.2 50.667 75 36 1 38 111 63 137 5.82e-18 81.3
Msa0924170 AT4G01540.1 50.667 75 36 1 38 111 63 137 6.56e-18 81.3
Msa0924170 AT4G01540.4 50.667 75 36 1 38 111 63 137 7.15e-18 80.5
Msa0924170 AT2G27300.1 37.391 115 66 2 36 150 68 176 7.46e-18 80.1
Msa0924170 AT3G44290.1 39.640 111 58 3 38 148 70 171 9.68e-18 79.7
Msa0924170 AT4G01520.1 49.333 75 37 1 38 111 63 137 1.06e-17 79.3
Msa0924170 AT2G43000.2 48.235 85 38 3 48 128 5 87 1.50e-17 77.0
Msa0924170 AT4G01550.1 40.351 114 55 4 40 152 64 165 1.51e-16 77.0
Msa0924170 AT1G02230.1 40.800 125 61 5 40 163 64 176 1.83e-16 76.6
Msa0924170 AT3G44350.2 41.489 94 50 2 41 130 68 160 5.55e-16 73.9
Msa0924170 AT5G04395.1 42.683 82 46 1 36 116 94 175 1.69e-15 71.6
Msa0924170 AT3G44350.1 45.333 75 40 1 41 115 68 141 2.74e-15 70.5
Msa0924170 AT3G04420.2 51.471 68 31 1 41 108 65 130 8.67e-15 71.2
Msa0924170 AT3G04420.1 51.471 68 31 1 41 108 65 130 1.11e-14 71.2
Msa0924170 AT5G22380.1 36.735 98 56 2 37 129 65 161 1.87e-14 69.7
Msa0924170 AT5G22290.2 35.965 114 60 4 62 175 2 102 2.83e-13 66.6
Msa0924170 AT1G02250.1 42.391 92 48 3 40 130 64 151 2.86e-13 67.4
Msa0924170 AT1G02220.1 47.059 68 31 2 41 108 65 127 1.00e-11 63.2
Msa0924170 AT4G28530.2 48.276 58 30 0 37 94 80 137 8.32e-11 60.1

Find 41 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGTCATATTAGCTTAT+TGG 0.203361 6_2:+65971152 Msa0924170:CDS
ATCACCTTCTGCAGCATATA+AGG 0.276669 6_2:-65970211 None:intergenic
CTGTTAGATCGGTTCCCATT+TGG 0.335676 6_2:-65970738 None:intergenic
GAGCTACAAACTCTGGTTAC+TGG 0.345872 6_2:+65970760 Msa0924170:CDS
TCGGTTCCCATTTGGATACT+TGG 0.352516 6_2:-65970730 None:intergenic
CTTGTATTTCTCCAAGTCAT+TGG 0.380097 6_2:+65971135 Msa0924170:CDS
GTGCTTGCTCTCCAAGTCAT+TGG 0.411683 6_2:+65971182 Msa0924170:CDS
TTACTGGAAGGCAACTGGTT+TGG 0.413426 6_2:+65970776 Msa0924170:CDS
CTTGGAGAGCAAGCACAATA+TGG 0.416309 6_2:-65971175 None:intergenic
AAAGAATCCAAGTATCCAAA+TGG 0.424681 6_2:+65970723 Msa0924170:CDS
GATTTCGACCTCCAGATGTA+AGG 0.424926 6_2:-65970183 None:intergenic
TCAAAGAATCGTGAAGTTGT+TGG 0.426965 6_2:+65970816 Msa0924170:CDS
TCTGGTTACTGGAAGGCAAC+TGG 0.438388 6_2:+65970771 Msa0924170:CDS
TGGTACTTCGTTGATTTGAT+TGG 0.447213 6_2:-65970086 None:intergenic
TGCAGAATGAAAATTGGGTT+CGG 0.450439 6_2:+65971029 Msa0924170:intron
GGTGATAGAGCCCTTACATC+TGG 0.457494 6_2:+65970172 Msa0924170:intron
ATGTATCAAAATATTCTTCG+TGG 0.472051 6_2:+65970052 Msa0924170:CDS
TACAAGTTGCTGCCACTGCT+TGG 0.479241 6_2:-65971118 None:intergenic
TTCCTCTCACTCCAACCCCT+TGG 0.484311 6_2:+65970923 Msa0924170:CDS
AGGAACAAGCACCAATGACT+TGG 0.511090 6_2:-65971193 None:intergenic
GGTTGGAGTGAGAGGAAGTG+AGG 0.514880 6_2:-65970917 None:intergenic
AGCTAATATGACCAATGACT+TGG 0.521909 6_2:-65971146 None:intergenic
AGTTTGTAGCTCTGTTAGAT+CGG 0.532621 6_2:-65970749 None:intergenic
AAGAATCCAAGTATCCAAAT+GGG 0.544681 6_2:+65970724 Msa0924170:CDS
AATGGAGAAGAGACAGTGAA+AGG 0.546634 6_2:+65971088 Msa0924170:CDS
GACCAAGGGGTTGGAGTGAG+AGG 0.548262 6_2:-65970925 None:intergenic
CTCATGGTTCAAGAACTGAT+TGG 0.548704 6_2:+65970877 Msa0924170:CDS
AGAAAGACTGACCAAGGGGT+TGG 0.548887 6_2:-65970934 None:intergenic
TCTAACAGAGCTACAAACTC+TGG 0.560803 6_2:+65970753 Msa0924170:CDS
ATTTCGACCTCCAGATGTAA+GGG 0.582611 6_2:-65970182 None:intergenic
TAGAGAAATACAAAGACTTG+AGG 0.596017 6_2:-65971213 None:intergenic
AAAGGAGTGAAGCCAAGCAG+TGG 0.603258 6_2:+65971106 Msa0924170:CDS
TACAGAGGGAAACCACCTCA+TGG 0.609880 6_2:+65970861 Msa0924170:CDS
CGCATATTTCTGAAGAGGAG+AGG 0.611502 6_2:+65971055 Msa0924170:CDS
ACTTCCTTATATGCTGCAGA+AGG 0.614507 6_2:+65970207 Msa0924170:CDS
TCAGTTCTTGAACCATGAGG+TGG 0.615010 6_2:-65970873 None:intergenic
GGTGTCGCATATTTCTGAAG+AGG 0.616757 6_2:+65971050 Msa0924170:CDS
TACAAACTCTGGTTACTGGA+AGG 0.635081 6_2:+65970764 Msa0924170:CDS
ATATTTCTGAAGAGGAGAGG+TGG 0.674268 6_2:+65971058 Msa0924170:CDS
GATAGAGCCCTTACATCTGG+AGG 0.683929 6_2:+65970175 Msa0924170:intron
CAATCAGTTCTTGAACCATG+AGG 0.707588 6_2:-65970876 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAATTGAAATTTGATA+TGG - chr6_2:65970113-65970132 None:intergenic 15.0%
!! TAATATCAATATGAATGAAT+TGG + chr6_2:65971002-65971021 Msa0924170:intron 15.0%
!! AATTCAATTGAAATTTGATA+TGG - chr6_2:65970113-65970132 None:intergenic 15.0%
!! TAATATCAATATGAATGAAT+TGG + chr6_2:65971002-65971021 Msa0924170:intron 15.0%
!!! GAAGAATATTTTGATACATA+TGG - chr6_2:65970051-65970070 None:intergenic 20.0%
!! ACAAATTATGGTAGAAATAA+AGG - chr6_2:65970252-65970271 None:intergenic 20.0%
!! CAAAATCATTAGACAAATTA+TGG - chr6_2:65970264-65970283 None:intergenic 20.0%
!!! GAAGAATATTTTGATACATA+TGG - chr6_2:65970051-65970070 None:intergenic 20.0%
!! ACAAATTATGGTAGAAATAA+AGG - chr6_2:65970252-65970271 None:intergenic 20.0%
!! CAAAATCATTAGACAAATTA+TGG - chr6_2:65970264-65970283 None:intergenic 20.0%
! ATGTATCAAAATATTCTTCG+TGG + chr6_2:65970052-65970071 Msa0924170:CDS 25.0%
! CAATTGAAATTTGATATGGT+TGG - chr6_2:65970109-65970128 None:intergenic 25.0%
!!! ATTGACATTTTTGTTGTTTC+TGG - chr6_2:65970532-65970551 None:intergenic 25.0%
! TTACAAATTCAGACAAAGAA+AGG - chr6_2:65970965-65970984 None:intergenic 25.0%
!! GATTTTGCAGAATGAAAATT+GGG + chr6_2:65971024-65971043 Msa0924170:intron 25.0%
! ATGTATCAAAATATTCTTCG+TGG + chr6_2:65970052-65970071 Msa0924170:CDS 25.0%
! CAATTGAAATTTGATATGGT+TGG - chr6_2:65970109-65970128 None:intergenic 25.0%
!!! ATTGACATTTTTGTTGTTTC+TGG - chr6_2:65970532-65970551 None:intergenic 25.0%
! TTACAAATTCAGACAAAGAA+AGG - chr6_2:65970965-65970984 None:intergenic 25.0%
!! GATTTTGCAGAATGAAAATT+GGG + chr6_2:65971024-65971043 Msa0924170:intron 25.0%
TTTCATGCTACCAAATAACA+GGG + chr6_2:65970470-65970489 Msa0924170:intron 30.0%
!!! GAGTTTTTTTCCCTGTTATT+TGG - chr6_2:65970483-65970502 None:intergenic 30.0%
! ATCTACAGATGATGCATTTT+CGG - chr6_2:65970574-65970593 None:intergenic 30.0%
GATTTATTGTGATCAATGCA+AGG - chr6_2:65970606-65970625 None:intergenic 30.0%
AAAGAATCCAAGTATCCAAA+TGG + chr6_2:65970723-65970742 Msa0924170:CDS 30.0%
AAGAATCCAAGTATCCAAAT+GGG + chr6_2:65970724-65970743 Msa0924170:CDS 30.0%
! AAAACTCATGTTTTCTACAG+AGG + chr6_2:65970846-65970865 Msa0924170:CDS 30.0%
! AAACTCATGTTTTCTACAGA+GGG + chr6_2:65970847-65970866 Msa0924170:CDS 30.0%
! GGATTTTGCAGAATGAAAAT+TGG + chr6_2:65971023-65971042 Msa0924170:intron 30.0%
! CATTGGTCATATTAGCTTAT+TGG + chr6_2:65971152-65971171 Msa0924170:CDS 30.0%
TAGAGAAATACAAAGACTTG+AGG - chr6_2:65971216-65971235 None:intergenic 30.0%
TTTCATGCTACCAAATAACA+GGG + chr6_2:65970470-65970489 Msa0924170:intron 30.0%
!!! GAGTTTTTTTCCCTGTTATT+TGG - chr6_2:65970483-65970502 None:intergenic 30.0%
! ATCTACAGATGATGCATTTT+CGG - chr6_2:65970574-65970593 None:intergenic 30.0%
GATTTATTGTGATCAATGCA+AGG - chr6_2:65970606-65970625 None:intergenic 30.0%
AAAGAATCCAAGTATCCAAA+TGG + chr6_2:65970723-65970742 Msa0924170:CDS 30.0%
AAGAATCCAAGTATCCAAAT+GGG + chr6_2:65970724-65970743 Msa0924170:CDS 30.0%
! AAAACTCATGTTTTCTACAG+AGG + chr6_2:65970846-65970865 Msa0924170:CDS 30.0%
! AAACTCATGTTTTCTACAGA+GGG + chr6_2:65970847-65970866 Msa0924170:CDS 30.0%
! GGATTTTGCAGAATGAAAAT+TGG + chr6_2:65971023-65971042 Msa0924170:intron 30.0%
! CATTGGTCATATTAGCTTAT+TGG + chr6_2:65971152-65971171 Msa0924170:CDS 30.0%
TAGAGAAATACAAAGACTTG+AGG - chr6_2:65971216-65971235 None:intergenic 30.0%
!! TGGTACTTCGTTGATTTGAT+TGG - chr6_2:65970089-65970108 None:intergenic 35.0%
TATTGCATCATCACTACGAA+TGG - chr6_2:65970378-65970397 None:intergenic 35.0%
! TTCGTAGTGATGATGCAATA+CGG + chr6_2:65970378-65970397 Msa0924170:intron 35.0%
GTTTCATGCTACCAAATAAC+AGG + chr6_2:65970469-65970488 Msa0924170:intron 35.0%
! TGAGTGTGTGTTGTTCTATT+AGG + chr6_2:65970680-65970699 Msa0924170:intron 35.0%
! AGTTTGTAGCTCTGTTAGAT+CGG - chr6_2:65970752-65970771 None:intergenic 35.0%
TCAAAGAATCGTGAAGTTGT+TGG + chr6_2:65970816-65970835 Msa0924170:CDS 35.0%
AAGAAAGAAAGACTGACCAA+GGG - chr6_2:65970942-65970961 None:intergenic 35.0%
TGCAGAATGAAAATTGGGTT+CGG + chr6_2:65971029-65971048 Msa0924170:intron 35.0%
CTTGTATTTCTCCAAGTCAT+TGG + chr6_2:65971135-65971154 Msa0924170:CDS 35.0%
AGCTAATATGACCAATGACT+TGG - chr6_2:65971149-65971168 None:intergenic 35.0%
!! TGGTACTTCGTTGATTTGAT+TGG - chr6_2:65970089-65970108 None:intergenic 35.0%
TATTGCATCATCACTACGAA+TGG - chr6_2:65970378-65970397 None:intergenic 35.0%
! TTCGTAGTGATGATGCAATA+CGG + chr6_2:65970378-65970397 Msa0924170:intron 35.0%
GTTTCATGCTACCAAATAAC+AGG + chr6_2:65970469-65970488 Msa0924170:intron 35.0%
! TGAGTGTGTGTTGTTCTATT+AGG + chr6_2:65970680-65970699 Msa0924170:intron 35.0%
! AGTTTGTAGCTCTGTTAGAT+CGG - chr6_2:65970752-65970771 None:intergenic 35.0%
TCAAAGAATCGTGAAGTTGT+TGG + chr6_2:65970816-65970835 Msa0924170:CDS 35.0%
AAGAAAGAAAGACTGACCAA+GGG - chr6_2:65970942-65970961 None:intergenic 35.0%
TGCAGAATGAAAATTGGGTT+CGG + chr6_2:65971029-65971048 Msa0924170:intron 35.0%
CTTGTATTTCTCCAAGTCAT+TGG + chr6_2:65971135-65971154 Msa0924170:CDS 35.0%
AGCTAATATGACCAATGACT+TGG - chr6_2:65971149-65971168 None:intergenic 35.0%
CATGACAATCAAGCAAATCC+CGG - chr6_2:65970153-65970172 None:intergenic 40.0%
ATTTCGACCTCCAGATGTAA+GGG - chr6_2:65970185-65970204 None:intergenic 40.0%
ACTTCCTTATATGCTGCAGA+AGG + chr6_2:65970207-65970226 Msa0924170:CDS 40.0%
ATCACCTTCTGCAGCATATA+AGG - chr6_2:65970214-65970233 None:intergenic 40.0%
TCTAACAGAGCTACAAACTC+TGG + chr6_2:65970753-65970772 Msa0924170:CDS 40.0%
TACAAACTCTGGTTACTGGA+AGG + chr6_2:65970764-65970783 Msa0924170:CDS 40.0%
CAATCAGTTCTTGAACCATG+AGG - chr6_2:65970879-65970898 None:intergenic 40.0%
CTCATGGTTCAAGAACTGAT+TGG + chr6_2:65970877-65970896 Msa0924170:CDS 40.0%
AGAAAGAAAGACTGACCAAG+GGG - chr6_2:65970941-65970960 None:intergenic 40.0%
GAAGAAAGAAAGACTGACCA+AGG - chr6_2:65970943-65970962 None:intergenic 40.0%
! ATATTTCTGAAGAGGAGAGG+TGG + chr6_2:65971058-65971077 Msa0924170:CDS 40.0%
AATGGAGAAGAGACAGTGAA+AGG + chr6_2:65971088-65971107 Msa0924170:CDS 40.0%
CATGACAATCAAGCAAATCC+CGG - chr6_2:65970153-65970172 None:intergenic 40.0%
ATTTCGACCTCCAGATGTAA+GGG - chr6_2:65970185-65970204 None:intergenic 40.0%
ACTTCCTTATATGCTGCAGA+AGG + chr6_2:65970207-65970226 Msa0924170:CDS 40.0%
ATCACCTTCTGCAGCATATA+AGG - chr6_2:65970214-65970233 None:intergenic 40.0%
TCTAACAGAGCTACAAACTC+TGG + chr6_2:65970753-65970772 Msa0924170:CDS 40.0%
TACAAACTCTGGTTACTGGA+AGG + chr6_2:65970764-65970783 Msa0924170:CDS 40.0%
CAATCAGTTCTTGAACCATG+AGG - chr6_2:65970879-65970898 None:intergenic 40.0%
CTCATGGTTCAAGAACTGAT+TGG + chr6_2:65970877-65970896 Msa0924170:CDS 40.0%
AGAAAGAAAGACTGACCAAG+GGG - chr6_2:65970941-65970960 None:intergenic 40.0%
GAAGAAAGAAAGACTGACCA+AGG - chr6_2:65970943-65970962 None:intergenic 40.0%
! ATATTTCTGAAGAGGAGAGG+TGG + chr6_2:65971058-65971077 Msa0924170:CDS 40.0%
AATGGAGAAGAGACAGTGAA+AGG + chr6_2:65971088-65971107 Msa0924170:CDS 40.0%
TTGAATCGCATGTCCTTGAC+CGG + chr6_2:65970131-65970150 Msa0924170:intron 45.0%
AATCAAGCAAATCCCGGTCA+AGG - chr6_2:65970147-65970166 None:intergenic 45.0%
! CGGGATTTGCTTGATTGTCA+TGG + chr6_2:65970151-65970170 Msa0924170:intron 45.0%
GATTTCGACCTCCAGATGTA+AGG - chr6_2:65970186-65970205 None:intergenic 45.0%
TCGGTTCCCATTTGGATACT+TGG - chr6_2:65970733-65970752 None:intergenic 45.0%
! CTGTTAGATCGGTTCCCATT+TGG - chr6_2:65970741-65970760 None:intergenic 45.0%
GAGCTACAAACTCTGGTTAC+TGG + chr6_2:65970760-65970779 Msa0924170:CDS 45.0%
TTACTGGAAGGCAACTGGTT+TGG + chr6_2:65970776-65970795 Msa0924170:CDS 45.0%
TCAGTTCTTGAACCATGAGG+TGG - chr6_2:65970876-65970895 None:intergenic 45.0%
! GGTGTCGCATATTTCTGAAG+AGG + chr6_2:65971050-65971069 Msa0924170:CDS 45.0%
! CGCATATTTCTGAAGAGGAG+AGG + chr6_2:65971055-65971074 Msa0924170:CDS 45.0%
! AGGAGAGGTGGTGCTAAAAA+TGG + chr6_2:65971070-65971089 Msa0924170:CDS 45.0%
CTTGGAGAGCAAGCACAATA+TGG - chr6_2:65971178-65971197 None:intergenic 45.0%
AGGAACAAGCACCAATGACT+TGG - chr6_2:65971196-65971215 None:intergenic 45.0%
TTGAATCGCATGTCCTTGAC+CGG + chr6_2:65970131-65970150 Msa0924170:intron 45.0%
AATCAAGCAAATCCCGGTCA+AGG - chr6_2:65970147-65970166 None:intergenic 45.0%
! CGGGATTTGCTTGATTGTCA+TGG + chr6_2:65970151-65970170 Msa0924170:intron 45.0%
GATTTCGACCTCCAGATGTA+AGG - chr6_2:65970186-65970205 None:intergenic 45.0%
TCGGTTCCCATTTGGATACT+TGG - chr6_2:65970733-65970752 None:intergenic 45.0%
! CTGTTAGATCGGTTCCCATT+TGG - chr6_2:65970741-65970760 None:intergenic 45.0%
GAGCTACAAACTCTGGTTAC+TGG + chr6_2:65970760-65970779 Msa0924170:CDS 45.0%
TTACTGGAAGGCAACTGGTT+TGG + chr6_2:65970776-65970795 Msa0924170:CDS 45.0%
TCAGTTCTTGAACCATGAGG+TGG - chr6_2:65970876-65970895 None:intergenic 45.0%
! GGTGTCGCATATTTCTGAAG+AGG + chr6_2:65971050-65971069 Msa0924170:CDS 45.0%
! CGCATATTTCTGAAGAGGAG+AGG + chr6_2:65971055-65971074 Msa0924170:CDS 45.0%
! AGGAGAGGTGGTGCTAAAAA+TGG + chr6_2:65971070-65971089 Msa0924170:CDS 45.0%
CTTGGAGAGCAAGCACAATA+TGG - chr6_2:65971178-65971197 None:intergenic 45.0%
AGGAACAAGCACCAATGACT+TGG - chr6_2:65971196-65971215 None:intergenic 45.0%
TGAATCGCATGTCCTTGACC+GGG + chr6_2:65970132-65970151 Msa0924170:intron 50.0%
GGTGATAGAGCCCTTACATC+TGG + chr6_2:65970172-65970191 Msa0924170:intron 50.0%
GATAGAGCCCTTACATCTGG+AGG + chr6_2:65970175-65970194 Msa0924170:intron 50.0%
CTGCGATGCTGCTACTAGAT+TGG + chr6_2:65970412-65970431 Msa0924170:intron 50.0%
TCTGGTTACTGGAAGGCAAC+TGG + chr6_2:65970771-65970790 Msa0924170:CDS 50.0%
TACAGAGGGAAACCACCTCA+TGG + chr6_2:65970861-65970880 Msa0924170:CDS 50.0%
AGAAAGACTGACCAAGGGGT+TGG - chr6_2:65970937-65970956 None:intergenic 50.0%
AAAGGAGTGAAGCCAAGCAG+TGG + chr6_2:65971106-65971125 Msa0924170:CDS 50.0%
TACAAGTTGCTGCCACTGCT+TGG - chr6_2:65971121-65971140 None:intergenic 50.0%
GTGCTTGCTCTCCAAGTCAT+TGG + chr6_2:65971182-65971201 Msa0924170:CDS 50.0%
TGAATCGCATGTCCTTGACC+GGG + chr6_2:65970132-65970151 Msa0924170:intron 50.0%
GGTGATAGAGCCCTTACATC+TGG + chr6_2:65970172-65970191 Msa0924170:intron 50.0%
GATAGAGCCCTTACATCTGG+AGG + chr6_2:65970175-65970194 Msa0924170:intron 50.0%
CTGCGATGCTGCTACTAGAT+TGG + chr6_2:65970412-65970431 Msa0924170:intron 50.0%
TCTGGTTACTGGAAGGCAAC+TGG + chr6_2:65970771-65970790 Msa0924170:CDS 50.0%
TACAGAGGGAAACCACCTCA+TGG + chr6_2:65970861-65970880 Msa0924170:CDS 50.0%
AGAAAGACTGACCAAGGGGT+TGG - chr6_2:65970937-65970956 None:intergenic 50.0%
AAAGGAGTGAAGCCAAGCAG+TGG + chr6_2:65971106-65971125 Msa0924170:CDS 50.0%
TACAAGTTGCTGCCACTGCT+TGG - chr6_2:65971121-65971140 None:intergenic 50.0%
GTGCTTGCTCTCCAAGTCAT+TGG + chr6_2:65971182-65971201 Msa0924170:CDS 50.0%
GGTTGGAGTGAGAGGAAGTG+AGG - chr6_2:65970920-65970939 None:intergenic 55.0%
TTCCTCTCACTCCAACCCCT+TGG + chr6_2:65970923-65970942 Msa0924170:CDS 55.0%
GGTTGGAGTGAGAGGAAGTG+AGG - chr6_2:65970920-65970939 None:intergenic 55.0%
TTCCTCTCACTCCAACCCCT+TGG + chr6_2:65970923-65970942 Msa0924170:CDS 55.0%
GACCAAGGGGTTGGAGTGAG+AGG - chr6_2:65970928-65970947 None:intergenic 60.0%
GACCAAGGGGTTGGAGTGAG+AGG - chr6_2:65970928-65970947 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 65970041 65971255 65970041 ID=Msa0924170;Name=Msa0924170
chr6_2 mRNA 65970041 65971255 65970041 ID=Msa0924170-mRNA-1;Parent=Msa0924170;Name=Msa0924170-mRNA-1;_AED=0.47;_eAED=0.55;_QI=0|0|0|1|1|1|4|0|191
chr6_2 exon 65970041 65970102 65970041 ID=Msa0924170-mRNA-1:exon:10391;Parent=Msa0924170-mRNA-1
chr6_2 exon 65970180 65970228 65970180 ID=Msa0924170-mRNA-1:exon:10392;Parent=Msa0924170-mRNA-1
chr6_2 exon 65970702 65970944 65970702 ID=Msa0924170-mRNA-1:exon:10393;Parent=Msa0924170-mRNA-1
chr6_2 exon 65971034 65971255 65971034 ID=Msa0924170-mRNA-1:exon:10394;Parent=Msa0924170-mRNA-1
chr6_2 CDS 65970041 65970102 65970041 ID=Msa0924170-mRNA-1:cds;Parent=Msa0924170-mRNA-1
chr6_2 CDS 65970180 65970228 65970180 ID=Msa0924170-mRNA-1:cds;Parent=Msa0924170-mRNA-1
chr6_2 CDS 65970702 65970944 65970702 ID=Msa0924170-mRNA-1:cds;Parent=Msa0924170-mRNA-1
chr6_2 CDS 65971034 65971255 65971034 ID=Msa0924170-mRNA-1:cds;Parent=Msa0924170-mRNA-1
Gene Sequence

>Msa0924170

ATGGATACCATATGTATCAAAATATTCTTCGTGGAATATCGAATTCCAATCAAATCAACGAAAGCCCTTACATCTGGAGGTCGAAATCAACTTCCTTATATGCTGCAGAAGGAGATGTACTTTTTCAGTACTAAAGAATCCAAGTATCCAAATGGGAACCGATCTAACAGAGCTACAAACTCTGGTTACTGGAAGGCAACTGGTTTGGACAAACAAATCATGAATTCAAAGAATCGTGAAGTTGTTGGTATGAAGAAAACTCATGTTTTCTACAGAGGGAAACCACCTCATGGTTCAAGAACTGATTGGATCATGCATGAATATCGCCTCACTTCCTCTCACTCCAACCCCTTGAATGAAAATTGGGTTCGGTGTCGCATATTTCTGAAGAGGAGAGGTGGTGCTAAAAATGGAGAAGAGACAGTGAAAGGAGTGAAGCCAAGCAGTGGCAGCAACTTGTATTTCTCCAAGTCATTGGTCATATTAGCTTATTGGCCATATTGTGCTTGCTCTCCAAGTCATTGGTGCTTGTTCCTCAAGTCTTTGTATTTCTCTATAAATAGAGCTCATATATAA
Protein sequence

>Msa0924170

MDTICIKIFFVEYRIPIKSTKALTSGGRNQLPYMLQKEMYFFSTKESKYPNGNRSNRATNSGYWKATGLDKQIMNSKNREVVGMKKTHVFYRGKPPHGSRTDWIMHEYRLTSSHSNPLNENWVRCRIFLKRRGGAKNGEETVKGVKPSSGSNLYFSKSLVILAYWPYCACSPSHWCLFLKSLYFSINRAHI*