Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0924710 | GAU30764.1 | 76.000 | 75 | 16 | 1 | 1 | 75 | 254 | 326 | 3.38e-26 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0924710 | TR | mTERF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0924710 | MtrunA17_Chr8g0339041 | 72.000 | 75 | 19 | 1 | 1 | 75 | 254 | 326 | 4.16e-28 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGACATTGTCAACTGATA+AGG | 0.410089 | 6_2:-67193576 | Msa0924710:CDS |
| TCACGCACACGCTCAATACT+TGG | 0.430841 | 6_2:-67193398 | Msa0924710:CDS |
| AGCAATGAAGATACAAGATA+TGG | 0.431980 | 6_2:-67193435 | Msa0924710:CDS |
| AACTGAAAAGTTCAAGTGAC+TGG | 0.468045 | 6_2:+67192433 | None:intergenic |
| TTCATTGCTGTAAGCACTCG+TGG | 0.533120 | 6_2:+67193449 | None:intergenic |
| TTTCTGCCATTGTGAAAACA+TGG | 0.586875 | 6_2:+67192466 | None:intergenic |
| CAGCACTAAGTGATTTGAGA+AGG | 0.593912 | 6_2:+67192490 | None:intergenic |
| CATTGAGGATGTCAGAGGAG+AGG | 0.607342 | 6_2:-67193373 | Msa0924710:intron |
| GCTCAATACTTGGAGCATTG+AGG | 0.621022 | 6_2:-67193388 | Msa0924710:CDS |
| TGTCAACTGATAAGGTTCAG+TGG | 0.649714 | 6_2:-67193568 | Msa0924710:CDS |
| TGGAGCATTGAGGATGTCAG+AGG | 0.663911 | 6_2:-67193378 | Msa0924710:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATTACTAAATATCTGAT+TGG | - | chr6_2:67193151-67193170 | Msa0924710:intron | 15.0% |
| !! | TTTGTGAAAATAATAGTAAA+GGG | - | chr6_2:67193313-67193332 | Msa0924710:intron | 15.0% |
| !! | TTTATTACTAAATATCTGAT+TGG | - | chr6_2:67193151-67193170 | Msa0924710:intron | 15.0% |
| !! | TTTGTGAAAATAATAGTAAA+GGG | - | chr6_2:67193313-67193332 | Msa0924710:intron | 15.0% |
| !! | TTGCTTATGATTTAATTCAA+TGG | - | chr6_2:67192687-67192706 | Msa0924710:intron | 20.0% |
| !! | TTTATAACTATTATTACCGA+AGG | - | chr6_2:67192955-67192974 | Msa0924710:intron | 20.0% |
| !! | TTTATAACTATTATTACCGA+AGG | - | chr6_2:67192955-67192974 | Msa0924710:intron | 20.0% |
| !! | TCAAATCCATAGTAAATTTA+AGG | - | chr6_2:67193257-67193276 | Msa0924710:intron | 20.0% |
| !! | CAAATCCATAGTAAATTTAA+GGG | - | chr6_2:67193258-67193277 | Msa0924710:intron | 20.0% |
| !! | GTTTGTGAAAATAATAGTAA+AGG | - | chr6_2:67193312-67193331 | Msa0924710:intron | 20.0% |
| !! | TAAGATACATAACATTGAAT+AGG | - | chr6_2:67193382-67193401 | Msa0924710:CDS | 20.0% |
| !! | AAGATACATAACATTGAATA+GGG | - | chr6_2:67193383-67193402 | Msa0924710:CDS | 20.0% |
| !! | TTGCTTATGATTTAATTCAA+TGG | - | chr6_2:67192687-67192706 | Msa0924710:intron | 20.0% |
| !! | TTTATAACTATTATTACCGA+AGG | - | chr6_2:67192955-67192974 | Msa0924710:intron | 20.0% |
| !! | TTTATAACTATTATTACCGA+AGG | - | chr6_2:67192955-67192974 | Msa0924710:intron | 20.0% |
| !! | TCAAATCCATAGTAAATTTA+AGG | - | chr6_2:67193257-67193276 | Msa0924710:intron | 20.0% |
| !! | CAAATCCATAGTAAATTTAA+GGG | - | chr6_2:67193258-67193277 | Msa0924710:intron | 20.0% |
| !! | GTTTGTGAAAATAATAGTAA+AGG | - | chr6_2:67193312-67193331 | Msa0924710:intron | 20.0% |
| !! | TAAGATACATAACATTGAAT+AGG | - | chr6_2:67193382-67193401 | Msa0924710:CDS | 20.0% |
| !! | AAGATACATAACATTGAATA+GGG | - | chr6_2:67193383-67193402 | Msa0924710:CDS | 20.0% |
| !! | TGGAACATTACTTTCATTTT+AGG | - | chr6_2:67192452-67192471 | Msa0924710:CDS | 25.0% |
| ! | TATCTGCAAACAAGAAAAAT+GGG | + | chr6_2:67192514-67192533 | None:intergenic | 25.0% |
| ! | ATATCTGCAAACAAGAAAAA+TGG | + | chr6_2:67192515-67192534 | None:intergenic | 25.0% |
| !!! | ATTTTTCTTGTTTGCAGATA+TGG | - | chr6_2:67192514-67192533 | Msa0924710:intron | 25.0% |
| ! | AGAAATGATTGAAACAACTA+TGG | + | chr6_2:67192734-67192753 | None:intergenic | 25.0% |
| ! | TCGTCATAATCTCAAAAAAT+TGG | + | chr6_2:67192905-67192924 | None:intergenic | 25.0% |
| ! | TACTAAATATCTGATTGGAT+TGG | - | chr6_2:67193156-67193175 | Msa0924710:intron | 25.0% |
| ! | TCAATCAAAGTTTGAATGAT+AGG | - | chr6_2:67193220-67193239 | Msa0924710:intron | 25.0% |
| ! | TTCTACCCTTAAATTTACTA+TGG | + | chr6_2:67193266-67193285 | None:intergenic | 25.0% |
| !!! | ATCTTATCCTTTATTTTCAG+TGG | + | chr6_2:67193368-67193387 | None:intergenic | 25.0% |
| !! | TGGAACATTACTTTCATTTT+AGG | - | chr6_2:67192452-67192471 | Msa0924710:CDS | 25.0% |
| ! | TATCTGCAAACAAGAAAAAT+GGG | + | chr6_2:67192514-67192533 | None:intergenic | 25.0% |
| ! | ATATCTGCAAACAAGAAAAA+TGG | + | chr6_2:67192515-67192534 | None:intergenic | 25.0% |
| !!! | ATTTTTCTTGTTTGCAGATA+TGG | - | chr6_2:67192514-67192533 | Msa0924710:intron | 25.0% |
| ! | AGAAATGATTGAAACAACTA+TGG | + | chr6_2:67192734-67192753 | None:intergenic | 25.0% |
| ! | TCGTCATAATCTCAAAAAAT+TGG | + | chr6_2:67192905-67192924 | None:intergenic | 25.0% |
| ! | TACTAAATATCTGATTGGAT+TGG | - | chr6_2:67193156-67193175 | Msa0924710:intron | 25.0% |
| ! | TCAATCAAAGTTTGAATGAT+AGG | - | chr6_2:67193220-67193239 | Msa0924710:intron | 25.0% |
| ! | TTCTACCCTTAAATTTACTA+TGG | + | chr6_2:67193266-67193285 | None:intergenic | 25.0% |
| !!! | ATCTTATCCTTTATTTTCAG+TGG | + | chr6_2:67193368-67193387 | None:intergenic | 25.0% |
| AGCAATGAAGATACAAGATA+TGG | - | chr6_2:67192565-67192584 | Msa0924710:intron | 30.0% | |
| !! | ATTCAGTAATTTGTTGCACA+CGG | + | chr6_2:67193186-67193205 | None:intergenic | 30.0% |
| TTCAGATCCACTGAAAATAA+AGG | - | chr6_2:67193358-67193377 | Msa0924710:intron | 30.0% | |
| AGCAATGAAGATACAAGATA+TGG | - | chr6_2:67192565-67192584 | Msa0924710:intron | 30.0% | |
| !! | ATTCAGTAATTTGTTGCACA+CGG | + | chr6_2:67193186-67193205 | None:intergenic | 30.0% |
| TTCAGATCCACTGAAAATAA+AGG | - | chr6_2:67193358-67193377 | Msa0924710:intron | 30.0% | |
| CATAAGCAATTTCCTTGTCA+TGG | + | chr6_2:67192676-67192695 | None:intergenic | 35.0% | |
| AAGTTCTGTATTCCAAACAG+AGG | - | chr6_2:67192799-67192818 | Msa0924710:intron | 35.0% | |
| !! | TTCAGTAATTTGTTGCACAC+GGG | + | chr6_2:67193185-67193204 | None:intergenic | 35.0% |
| !! | ATTTTCAGTGGATCTGAAGT+TGG | + | chr6_2:67193356-67193375 | None:intergenic | 35.0% |
| TTTCTGCCATTGTGAAAACA+TGG | + | chr6_2:67193537-67193556 | None:intergenic | 35.0% | |
| AACTGAAAAGTTCAAGTGAC+TGG | + | chr6_2:67193570-67193589 | None:intergenic | 35.0% | |
| CATAAGCAATTTCCTTGTCA+TGG | + | chr6_2:67192676-67192695 | None:intergenic | 35.0% | |
| AAGTTCTGTATTCCAAACAG+AGG | - | chr6_2:67192799-67192818 | Msa0924710:intron | 35.0% | |
| !! | TTCAGTAATTTGTTGCACAC+GGG | + | chr6_2:67193185-67193204 | None:intergenic | 35.0% |
| !! | ATTTTCAGTGGATCTGAAGT+TGG | + | chr6_2:67193356-67193375 | None:intergenic | 35.0% |
| TTTCTGCCATTGTGAAAACA+TGG | + | chr6_2:67193537-67193556 | None:intergenic | 35.0% | |
| AACTGAAAAGTTCAAGTGAC+TGG | + | chr6_2:67193570-67193589 | None:intergenic | 35.0% | |
| TGTCAACTGATAAGGTTCAG+TGG | - | chr6_2:67192432-67192451 | Msa0924710:CDS | 40.0% | |
| AATTGTCCAATGCCATGACA+AGG | - | chr6_2:67192661-67192680 | Msa0924710:intron | 40.0% | |
| CAATTTCCTTGTCATGGCAT+TGG | + | chr6_2:67192670-67192689 | None:intergenic | 40.0% | |
| TCAGTTCACTTTCACTCCTT+CGG | + | chr6_2:67192974-67192993 | None:intergenic | 40.0% | |
| TCAGTTCACTTTCACTCCTT+CGG | + | chr6_2:67192974-67192993 | None:intergenic | 40.0% | |
| TAAGATCGAGTGAAGACTAC+AGG | - | chr6_2:67193412-67193431 | Msa0924710:CDS | 40.0% | |
| AAGATCGAGTGAAGACTACA+GGG | - | chr6_2:67193413-67193432 | Msa0924710:CDS | 40.0% | |
| ATGTAGTCTTCACTCGAGTA+CGG | + | chr6_2:67193453-67193472 | None:intergenic | 40.0% | |
| CAGCACTAAGTGATTTGAGA+AGG | + | chr6_2:67193513-67193532 | None:intergenic | 40.0% | |
| ! | TGCTGACCATGTTTTCACAA+TGG | - | chr6_2:67193528-67193547 | Msa0924710:CDS | 40.0% |
| TGTCAACTGATAAGGTTCAG+TGG | - | chr6_2:67192432-67192451 | Msa0924710:CDS | 40.0% | |
| AATTGTCCAATGCCATGACA+AGG | - | chr6_2:67192661-67192680 | Msa0924710:intron | 40.0% | |
| CAATTTCCTTGTCATGGCAT+TGG | + | chr6_2:67192670-67192689 | None:intergenic | 40.0% | |
| TCAGTTCACTTTCACTCCTT+CGG | + | chr6_2:67192974-67192993 | None:intergenic | 40.0% | |
| TCAGTTCACTTTCACTCCTT+CGG | + | chr6_2:67192974-67192993 | None:intergenic | 40.0% | |
| TAAGATCGAGTGAAGACTAC+AGG | - | chr6_2:67193412-67193431 | Msa0924710:CDS | 40.0% | |
| AAGATCGAGTGAAGACTACA+GGG | - | chr6_2:67193413-67193432 | Msa0924710:CDS | 40.0% | |
| ATGTAGTCTTCACTCGAGTA+CGG | + | chr6_2:67193453-67193472 | None:intergenic | 40.0% | |
| CAGCACTAAGTGATTTGAGA+AGG | + | chr6_2:67193513-67193532 | None:intergenic | 40.0% | |
| ! | TGCTGACCATGTTTTCACAA+TGG | - | chr6_2:67193528-67193547 | Msa0924710:CDS | 40.0% |
| TTCATTGCTGTAAGCACTCG+TGG | + | chr6_2:67192554-67192573 | None:intergenic | 45.0% | |
| !! | GCTCAATACTTGGAGCATTG+AGG | - | chr6_2:67192612-67192631 | Msa0924710:intron | 45.0% |
| TTCATTGCTGTAAGCACTCG+TGG | + | chr6_2:67192554-67192573 | None:intergenic | 45.0% | |
| !! | GCTCAATACTTGGAGCATTG+AGG | - | chr6_2:67192612-67192631 | Msa0924710:intron | 45.0% |
| TCACGCACACGCTCAATACT+TGG | - | chr6_2:67192602-67192621 | Msa0924710:intron | 50.0% | |
| !! | TGGAGCATTGAGGATGTCAG+AGG | - | chr6_2:67192622-67192641 | Msa0924710:intron | 50.0% |
| CATTGAGGATGTCAGAGGAG+AGG | - | chr6_2:67192627-67192646 | Msa0924710:intron | 50.0% | |
| ! | ACAGAAGTTGCAGGACTAGC+TGG | - | chr6_2:67192831-67192850 | Msa0924710:intron | 50.0% |
| GCTTGGATACTCGCAGTTTC+AGG | - | chr6_2:67192853-67192872 | Msa0924710:intron | 50.0% | |
| GTGAAGACTACAGGGACAAC+AGG | - | chr6_2:67193421-67193440 | Msa0924710:CDS | 50.0% | |
| TCACGCACACGCTCAATACT+TGG | - | chr6_2:67192602-67192621 | Msa0924710:intron | 50.0% | |
| !! | TGGAGCATTGAGGATGTCAG+AGG | - | chr6_2:67192622-67192641 | Msa0924710:intron | 50.0% |
| CATTGAGGATGTCAGAGGAG+AGG | - | chr6_2:67192627-67192646 | Msa0924710:intron | 50.0% | |
| ! | ACAGAAGTTGCAGGACTAGC+TGG | - | chr6_2:67192831-67192850 | Msa0924710:intron | 50.0% |
| GCTTGGATACTCGCAGTTTC+AGG | - | chr6_2:67192853-67192872 | Msa0924710:intron | 50.0% | |
| GTGAAGACTACAGGGACAAC+AGG | - | chr6_2:67193421-67193440 | Msa0924710:CDS | 50.0% | |
| TGTGAGTGCGCTCCTCTGTT+TGG | + | chr6_2:67192814-67192833 | None:intergenic | 55.0% | |
| AGCGCACTCACAGAAGTTGC+AGG | - | chr6_2:67192822-67192841 | Msa0924710:intron | 55.0% | |
| !! | AGTTGCAGGACTAGCTGGCT+TGG | - | chr6_2:67192836-67192855 | Msa0924710:intron | 55.0% |
| TGTGAGTGCGCTCCTCTGTT+TGG | + | chr6_2:67192814-67192833 | None:intergenic | 55.0% | |
| AGCGCACTCACAGAAGTTGC+AGG | - | chr6_2:67192822-67192841 | Msa0924710:intron | 55.0% | |
| !! | AGTTGCAGGACTAGCTGGCT+TGG | - | chr6_2:67192836-67192855 | Msa0924710:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 67192425 | 67193597 | 67192425 | ID=Msa0924710;Name=Msa0924710 |
| chr6_2 | mRNA | 67192425 | 67193597 | 67192425 | ID=Msa0924710-mRNA-1;Parent=Msa0924710;Name=Msa0924710-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|0|0.5|2|0|107 |
| chr6_2 | exon | 67193374 | 67193597 | 67193374 | ID=Msa0924710-mRNA-1:exon:10619;Parent=Msa0924710-mRNA-1 |
| chr6_2 | exon | 67192425 | 67192521 | 67192425 | ID=Msa0924710-mRNA-1:exon:10618;Parent=Msa0924710-mRNA-1 |
| chr6_2 | CDS | 67193374 | 67193597 | 67193374 | ID=Msa0924710-mRNA-1:cds;Parent=Msa0924710-mRNA-1 |
| chr6_2 | CDS | 67192425 | 67192521 | 67192425 | ID=Msa0924710-mRNA-1:cds;Parent=Msa0924710-mRNA-1 |
| Gene Sequence |
| Protein sequence |
