AlfalfaGEDB | Msa0927180

M. sativa cultivar zhongmu-4 / Msa0927180

KEGG: K04730|K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0927180	KAG5007665.1	57.143	287	57	5	113	355	295	559	4.20e-89	294

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0927180	sp\|Q9LV48\|PERK1_ARATH	47.022	319	91	9	113	355	283	599	2.34e-76	251

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0927180	MtrunA17_Chr6g0485721	52.012	323	79	8	113	363	101	419	1.93e-90	278
Msa0927180	MtrunA17_Chr6g0485761	48.148	324	79	7	113	355	298	613	2.85e-78	253
Msa0927180	MtrunA17_Chr5g0403891	60.656	183	31	2	113	274	259	421	1.54e-66	221
Msa0927180	MtrunA17_Chr3g0144481	40.694	317	113	6	114	355	93	409	6.02e-66	215
Msa0927180	MtrunA17_Chr8g0373501	60.440	182	31	2	114	274	341	502	5.54e-65	218
Msa0927180	MtrunA17_Chr8g0365691	49.333	225	67	4	77	274	273	477	1.18e-56	192
Msa0927180	MtrunA17_Chr5g0413511	53.297	182	44	2	114	274	63	224	2.05e-56	189
Msa0927180	MtrunA17_Chr7g0221191	52.747	182	45	2	114	274	399	560	5.20e-53	187
Msa0927180	MtrunA17_Chr0c01g0489481	52.198	182	46	2	114	274	335	496	4.26e-49	175
Msa0927180	MtrunA17_Chr4g0034931	41.622	185	65	2	113	274	143	307	1.43e-39	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0927180	AT3G24550.1	47.022	319	91	9	113	355	283	599	2.38e-77	251
Msa0927180	AT3G24540.1	43.729	295	93	5	113	336	186	478	4.09e-66	217
Msa0927180	AT2G18470.1	43.711	318	102	8	113	355	287	602	4.56e-65	218
Msa0927180	AT2G18470.3	43.711	318	102	8	113	355	287	602	4.56e-65	218
Msa0927180	AT2G18470.2	43.711	318	102	8	113	355	287	602	4.56e-65	218
Msa0927180	AT1G49270.1	63.536	181	24	2	116	274	342	502	5.35e-65	219
Msa0927180	AT4G34440.2	60.656	183	31	2	113	274	315	477	3.36e-64	216
Msa0927180	AT4G34440.1	60.656	183	31	2	113	274	315	477	3.36e-64	216
Msa0927180	AT2G18470.4	61.202	183	30	2	113	274	287	449	1.80e-63	211
Msa0927180	AT1G52290.2	54.645	183	42	2	113	274	146	308	9.79e-60	197

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Find 91 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGCATCAGACATCAA+AGG	0.543009	6_2:-71370087	Msa0927180:CDS
AAATAAAAGCCTTAAAGAGT+AGG	0.535452	6_2:+71369841	None:intergenic
AAATAGCATCAGACATCAAA+GGG	0.491223	6_2:-71370086	Msa0927180:CDS
AAATCTAAATCTTAATCAAG+GGG	0.619760	6_2:-71370187	Msa0927180:CDS
AAGAAGCTGAAAGCTGGTAG+CGG	0.600019	6_2:-71373142	Msa0927180:CDS
AAGGACGAACAACAATGGAT+TGG	0.587178	6_2:-71372942	Msa0927180:CDS
AATGCAATGCTTGAAGAAGA+AGG	0.556304	6_2:+71373305	None:intergenic
AATGGAAATGAGAACAGTCA+AGG	0.618015	6_2:-71370056	Msa0927180:exon
AATTCAATTGTACCATCCAT+AGG	0.555806	6_2:+71369951	None:intergenic
ACAAGACTGCAAATTGCTTT+AGG	0.305529	6_2:-71372916	Msa0927180:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTTGTTCCTAACAATACAT+TGG	-	chr6_2:71370320-71370339	Msa0927180:intron	30.0%
TGGAATTCCAATGTATTGTT+AGG	+	chr6_2:71370330-71370349	None:intergenic	30.0%
ACATTGGAATTCCATTTACA+CGG	-	chr6_2:71370336-71370355	Msa0927180:intron	30.0%
TCAAAAATGTACCGTGTAAA+TGG	+	chr6_2:71370350-71370369	None:intergenic	30.0%
ATTCCTTTCCTCTCAAAATT+TGG	-	chr6_2:71370813-71370832	Msa0927180:intron	30.0%
TCCTCTCAAAATTTGGATAT+CGG	-	chr6_2:71370820-71370839	Msa0927180:intron	30.0%
AGATACACACAAAAATGAGT+CGG	+	chr6_2:71371239-71371258	None:intergenic	30.0%
TAAAAGTTAGGGTTATGCTA+GGG	+	chr6_2:71371407-71371426	None:intergenic	30.0%
TTAAAAGTTAGGGTTATGCT+AGG	+	chr6_2:71371408-71371427	None:intergenic	30.0%
GATGGTTGTAGTTAAAAGTT+AGG	+	chr6_2:71371419-71371438	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	71369745	71373599	71369745	ID=Msa0927180;Name=Msa0927180
chr6_2	mRNA	71369745	71373599	71369745	ID=Msa0927180-mRNA-1;Parent=Msa0927180;Name=Msa0927180-mRNA-1;_AED=0.13;_eAED=0.13;_QI=24\|0.6\|0.66\|1\|1\|1\|6\|323\|365
chr6_2	exon	71369745	71370244	71369745	ID=Msa0927180-mRNA-1:exon:11874;Parent=Msa0927180-mRNA-1
chr6_2	exon	71371736	71371835	71371736	ID=Msa0927180-mRNA-1:exon:11873;Parent=Msa0927180-mRNA-1
chr6_2	exon	71372546	71372622	71372546	ID=Msa0927180-mRNA-1:exon:11872;Parent=Msa0927180-mRNA-1
chr6_2	exon	71372661	71372791	71372661	ID=Msa0927180-mRNA-1:exon:11871;Parent=Msa0927180-mRNA-1
chr6_2	exon	71372881	71372967	71372881	ID=Msa0927180-mRNA-1:exon:11870;Parent=Msa0927180-mRNA-1
chr6_2	exon	71373050	71373599	71373050	ID=Msa0927180-mRNA-1:exon:11869;Parent=Msa0927180-mRNA-1
chr6_2	five_prime_UTR	71373576	71373599	71373576	ID=Msa0927180-mRNA-1:five_prime_utr;Parent=Msa0927180-mRNA-1
chr6_2	CDS	71373050	71373575	71373050	ID=Msa0927180-mRNA-1:cds;Parent=Msa0927180-mRNA-1
chr6_2	CDS	71372881	71372967	71372881	ID=Msa0927180-mRNA-1:cds;Parent=Msa0927180-mRNA-1
chr6_2	CDS	71372661	71372791	71372661	ID=Msa0927180-mRNA-1:cds;Parent=Msa0927180-mRNA-1
chr6_2	CDS	71372546	71372622	71372546	ID=Msa0927180-mRNA-1:cds;Parent=Msa0927180-mRNA-1
chr6_2	CDS	71371736	71371835	71371736	ID=Msa0927180-mRNA-1:cds;Parent=Msa0927180-mRNA-1
chr6_2	CDS	71370068	71370244	71370068	ID=Msa0927180-mRNA-1:cds;Parent=Msa0927180-mRNA-1
chr6_2	three_prime_UTR	71369745	71370067	71369745	ID=Msa0927180-mRNA-1:three_prime_utr;Parent=Msa0927180-mRNA-1

Gene Sequence

>Msa0927180

ATGATTCAATTCCATCAGGACATCTGCCTCGTGCTTTTCTATCGCGGTCTAATGCTTTTCTACCAAGATGTCTGTCACCGCCATCATCGCCACCTCATGCTTCAATTCCATCAGGACATCTGCATCGTGCTTTTCTATCAAGACGTCTGTCACCGCCATCATCGCCTCATGGTTCTCTATCAGGACGTCTGTCACCTCCATCATTGCCGCCTCATGCTCCAATTCCATCAGGGGGCGAACTGCTTCCACCTTCTTCTTCAAGCATTGCATTTGGCTCCAGCAAGAGCACGTTTAACTATGAGGAGTTCGTGCGTGCAACGGATTGCTTCTCTGATCGCTAACTACCTAGGAATAGGGGGATTTGGATCTGTACACAAAGGTATTCTCCCTGATGGTAAAGTGGTTGCTATTAAGAAGCTGAAAGCTGGTAGCGGACAAGGGGAACGCGAATTTCAAGCTGAAGTTGGGATTATTAGCCGTGTTCATCACAGACATCTTGTCTCTCTTGTTGGATACTGCAGCACTGGACAAGGACGAACAACAATGGATTGGTCAACAAGACTGCAAATTGCTTTAGGATCCGCAAAAGGACTTGCATATCTTCATGATGATTGTCATCCTAAGATCATTCACCGTGATATTAAAGCTGCTAACATCCTTCTTGATTTTATGTTTGAAGCAAAGGAATATTCTATTACACGTCTTCCTTCTGGTAGTGATGATGCACTGTTTAAAAAATTCTTGGTTGCAGATTTTGGTATTGCAAAGACTACTTCTGATTTAAATACTCATGTTTCTACTCGAGTGATGGGAACTTTCGGGCTTCAGAATGAATTTGACCGTAATGAGATGACTCGTATGGTAGCATGCGCTGCAGCTTGCACACGTCAGTCAGCAAAGGATCGACCAAAGATGAGCCAGGTTGTGCGAGTTCTGGAAGGAGCTTTGTCAATAGCAAATCTAAATCTTAATCAAGGGGTTACACGTGGACCCAGCAGGGGTTACAGCAGAGACTACAATAGTCAGCAAGAAAACATGACAAAGTTGAAGTTGAAAAAAATAGCATCAGACATCAAAGGGAAATTGAAATGGAAATGA

Protein sequence

>Msa0927180

MIQFHQDICLVLFYRGLMLFYQDVCHRHHRHLMLQFHQDICIVLFYQDVCHRHHRLMVLYQDVCHLHHCRLMLQFHQGANCFHLLLQALHLAPARARLTMRSSCVQRIASLIANYLGIGGFGSVHKGILPDGKVVAIKKLKAGSGQGEREFQAEVGIISRVHHRHLVSLVGYCSTGQGRTTMDWSTRLQIALGSAKGLAYLHDDCHPKIIHRDIKAANILLDFMFEAKEYSITRLPSGSDDALFKKFLVADFGIAKTTSDLNTHVSTRVMGTFGLQNEFDRNEMTRMVACAAACTRQSAKDRPKMSQVVRVLEGALSIANLNLNQGVTRGPSRGYSRDYNSQQENMTKLKLKKIASDIKGKLKWK*