Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0928630 | AES61431.2 | 92.661 | 218 | 16 | 0 | 1 | 218 | 1 | 218 | 1.15e-149 | 427 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0928630 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0928630 | MtrunA17_Chr1g0172971 | 75.758 | 132 | 17 | 1 | 1 | 132 | 1 | 117 | 7.72e-62 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 6_2:+74461688 | None:intergenic |
| TTTGTTGTTGGCCTGTTGTT+AGG | 0.317810 | 6_2:+74461879 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 6_2:-74461613 | Msa0928630:CDS |
| ACCTGTGGACTGTTTGTTGT+TGG | 0.414676 | 6_2:+74461867 | None:intergenic |
| GTCTTATGAAGTTATGGGTC+AGG | 0.415385 | 6_2:-74461607 | Msa0928630:CDS |
| GAAGTTATGGGTCAGGGAGT+TGG | 0.426260 | 6_2:-74461600 | Msa0928630:CDS |
| CGTTATGTTTGTAACAAAGA+AGG | 0.427485 | 6_2:-74461927 | Msa0928630:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6_2:-74461675 | Msa0928630:CDS |
| TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 6_2:+74462090 | None:intergenic |
| TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 6_2:+74462089 | None:intergenic |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 6_2:-74461810 | Msa0928630:CDS |
| ATGTGTATAAGATATATAGA+TGG | 0.452819 | 6_2:-74461758 | Msa0928630:CDS |
| ACATCAACTTCATCTGATAG+TGG | 0.459514 | 6_2:-74462215 | Msa0928630:CDS |
| GCTCTTATAAATTCTAAAGC+TGG | 0.462891 | 6_2:-74461645 | Msa0928630:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 6_2:-74461612 | Msa0928630:CDS |
| GTTTACTTAATCCTAACAAC+AGG | 0.470860 | 6_2:-74461890 | Msa0928630:CDS |
| CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 6_2:-74462091 | Msa0928630:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 6_2:+74461816 | None:intergenic |
| AGCTGGAATATATTTGAAGC+TGG | 0.507410 | 6_2:-74461793 | Msa0928630:CDS |
| ACAAAACAAGAAATCTGATA+AGG | 0.511435 | 6_2:-74461961 | Msa0928630:CDS |
| CTAAGGTAACACGAATGCTC+TGG | 0.517941 | 6_2:+74461705 | None:intergenic |
| GTCAGCATGAATCATTGCTA+GGG | 0.522802 | 6_2:+74462130 | None:intergenic |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 6_2:-74462023 | Msa0928630:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 6_2:-74461916 | Msa0928630:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.528498 | 6_2:-74461751 | Msa0928630:CDS |
| GAATGCTCTGGTTTATGTAA+TGG | 0.534744 | 6_2:+74461717 | None:intergenic |
| CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 6_2:+74462091 | None:intergenic |
| TGTCAGCATGAATCATTGCT+AGG | 0.540740 | 6_2:+74462129 | None:intergenic |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 6_2:-74461915 | Msa0928630:CDS |
| GTTATGGGTCAGGGAGTTGG+AGG | 0.547221 | 6_2:-74461597 | Msa0928630:CDS |
| TGTGTATAAGATATATAGAT+GGG | 0.601002 | 6_2:-74461757 | Msa0928630:CDS |
| TCACTGATTCATGCTGAGAG+TGG | 0.606950 | 6_2:-74462170 | Msa0928630:CDS |
| TCTTATGAAGTTATGGGTCA+GGG | 0.609153 | 6_2:-74461606 | Msa0928630:CDS |
| GCCAACAACAAACAGTCCAC+AGG | 0.618105 | 6_2:-74461868 | Msa0928630:CDS |
| TTCACAAACTCAATAACCTG+TGG | 0.626760 | 6_2:+74461852 | None:intergenic |
| TCAGCATGAATCATTGCTAG+GGG | 0.681156 | 6_2:+74462131 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | chr6_2:74461770-74461789 | Msa0928630:CDS | 20.0% |
| !! | ATGTGTATAAGATATATAGA+TGG | - | chr6_2:74462056-74462075 | Msa0928630:CDS | 20.0% |
| !! | TGTGTATAAGATATATAGAT+GGG | - | chr6_2:74462057-74462076 | Msa0928630:CDS | 20.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | - | chr6_2:74461804-74461823 | Msa0928630:CDS | 25.0% |
| ! | ACAAAACAAGAAATCTGATA+AGG | - | chr6_2:74461853-74461872 | Msa0928630:CDS | 25.0% |
| TCCATGATTCTTTATTGCTA+GGG | + | chr6_2:74461727-74461746 | None:intergenic | 30.0% | |
| TTCCATGATTCTTTATTGCT+AGG | + | chr6_2:74461728-74461747 | None:intergenic | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | chr6_2:74461791-74461810 | Msa0928630:CDS | 30.0% | |
| CGTTATGTTTGTAACAAAGA+AGG | - | chr6_2:74461887-74461906 | Msa0928630:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | chr6_2:74461898-74461917 | Msa0928630:CDS | 30.0% | |
| AACAAAGAAGGTTTCAAGAA+GGG | - | chr6_2:74461899-74461918 | Msa0928630:CDS | 30.0% | |
| GTTTACTTAATCCTAACAAC+AGG | - | chr6_2:74461924-74461943 | Msa0928630:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr6_2:74462063-74462082 | Msa0928630:CDS | 30.0% | |
| TTACTTCTCGAAATGATCTA+AGG | + | chr6_2:74462129-74462148 | None:intergenic | 30.0% | |
| !! | GCTCTTATAAATTCTAAAGC+TGG | - | chr6_2:74462169-74462188 | Msa0928630:CDS | 30.0% |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr6_2:74462202-74462221 | Msa0928630:CDS | 30.0% | |
| ACATCAACTTCATCTGATAG+TGG | - | chr6_2:74461599-74461618 | Msa0928630:CDS | 35.0% | |
| CCATGATTCTTTATTGCTAG+GGG | + | chr6_2:74461726-74461745 | None:intergenic | 35.0% | |
| TTCACAAACTCAATAACCTG+TGG | + | chr6_2:74461965-74461984 | None:intergenic | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | chr6_2:74461982-74462001 | Msa0928630:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | chr6_2:74461987-74462006 | Msa0928630:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | - | chr6_2:74461988-74462007 | Msa0928630:CDS | 35.0% | |
| ! | AGCTGGAATATATTTGAAGC+TGG | - | chr6_2:74462021-74462040 | Msa0928630:CDS | 35.0% |
| ! | GAATGCTCTGGTTTATGTAA+TGG | + | chr6_2:74462100-74462119 | None:intergenic | 35.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr6_2:74462139-74462158 | Msa0928630:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr6_2:74462201-74462220 | Msa0928630:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+GGG | - | chr6_2:74462208-74462227 | Msa0928630:CDS | 35.0% | |
| TCAGCATGAATCATTGCTAG+GGG | + | chr6_2:74461686-74461705 | None:intergenic | 40.0% | |
| GTCAGCATGAATCATTGCTA+GGG | + | chr6_2:74461687-74461706 | None:intergenic | 40.0% | |
| TGTCAGCATGAATCATTGCT+AGG | + | chr6_2:74461688-74461707 | None:intergenic | 40.0% | |
| CCCCTAGCAATAAAGAATCA+TGG | - | chr6_2:74461723-74461742 | Msa0928630:CDS | 40.0% | |
| !! | TTTGTTGTTGGCCTGTTGTT+AGG | + | chr6_2:74461938-74461957 | None:intergenic | 40.0% |
| GTCTTATGAAGTTATGGGTC+AGG | - | chr6_2:74462207-74462226 | Msa0928630:CDS | 40.0% | |
| !! | TCACTGATTCATGCTGAGAG+TGG | - | chr6_2:74461644-74461663 | Msa0928630:CDS | 45.0% |
| !! | ACCTGTGGACTGTTTGTTGT+TGG | + | chr6_2:74461950-74461969 | None:intergenic | 45.0% |
| GAAAAACCTGAGGAAAGGGT+TGG | - | chr6_2:74461992-74462011 | Msa0928630:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | + | chr6_2:74462001-74462020 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr6_2:74462004-74462023 | Msa0928630:CDS | 45.0% |
| CTAAGGTAACACGAATGCTC+TGG | + | chr6_2:74462112-74462131 | None:intergenic | 45.0% | |
| GCCAACAACAAACAGTCCAC+AGG | - | chr6_2:74461946-74461965 | Msa0928630:CDS | 50.0% | |
| GAAGTTATGGGTCAGGGAGT+TGG | - | chr6_2:74462214-74462233 | Msa0928630:CDS | 50.0% | |
| GTTATGGGTCAGGGAGTTGG+AGG | - | chr6_2:74462217-74462236 | Msa0928630:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 74461590 | 74462246 | 74461590 | ID=Msa0928630;Name=Msa0928630 |
| chr6_2 | mRNA | 74461590 | 74462246 | 74461590 | ID=Msa0928630-mRNA-1;Parent=Msa0928630;Name=Msa0928630-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|218 |
| chr6_2 | exon | 74461590 | 74462246 | 74461590 | ID=Msa0928630-mRNA-1:exon:12687;Parent=Msa0928630-mRNA-1 |
| chr6_2 | CDS | 74461590 | 74462246 | 74461590 | ID=Msa0928630-mRNA-1:cds;Parent=Msa0928630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
