AlfalfaGEDB | Msa0929580

M. sativa cultivar zhongmu-4 / Msa0929580

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0929580	RHN51853.1	32.184	174	69	2	9	182	9	133	3.69e-16	93.2
Msa0929580	RHN51853.1	37.423	163	89	1	34	183	222	384	4.65e-20	105
Msa0929580	RHN51853.1	43.682	277	142	2	203	466	225	500	1.27e-74	253

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0929580	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0929580	MtrunA17_Chr6g0473661	43.682	277	142	2	203	466	225	500	1.17e-78	253
Msa0929580	MtrunA17_Chr6g0473661	37.423	163	89	1	34	183	222	384	4.27e-24	105
Msa0929580	MtrunA17_Chr6g0473661	32.184	174	69	2	9	182	9	133	3.40e-20	93.2
Msa0929580	MtrunA17_Chr6g0473651	49.640	139	69	1	212	350	216	353	1.47e-36	139
Msa0929580	MtrunA17_Chr6g0473651	44.531	128	71	0	56	183	226	353	2.04e-24	104
Msa0929580	MtrunA17_Chr6g0473651	31.214	173	108	3	279	443	23	192	3.18e-23	101
Msa0929580	MtrunA17_Chr6g0473651	47.887	71	37	0	112	182	22	92	8.05e-13	70.1
Msa0929580	MtrunA17_Chr6g0473921	36.957	138	87	0	328	465	1	138	8.28e-32	119
Msa0929580	MtrunA17_Chr1g0156191	37.500	136	84	1	294	429	1	135	1.55e-27	107
Msa0929580	MtrunA17_Chr1g0206471	29.767	215	139	2	207	410	38	251	1.38e-26	108

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 117 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACGACGGTCCAGTT+TGG	0.405460	6_2:-77706197	None:intergenic
AAACCCACCTAAGTCAGACA+TGG	0.685362	6_2:-77704882	None:intergenic
AAACCTATGGTCAGCGGCAG+TGG	0.455398	6_2:+77708155	Msa0929580:CDS
AAACTGGACCGTCGTGATTT+TGG	0.142859	6_2:+77706199	Msa0929580:CDS
AAATCTGTTGTTGATCGTCA+AGG	0.579813	6_2:-77706131	None:intergenic
AACCAGACTTTGCCGTGGTA+TGG	0.440028	6_2:+77707870	Msa0929580:intron
AACGCTTTGGATGCTGCACA+AGG	0.608033	6_2:+77704917	Msa0929580:CDS
AACGTATACCCCAAGTGAGA+TGG	0.579805	6_2:+77705743	Msa0929580:CDS
AATATGCCTTATGCTATGAT+TGG	0.515256	6_2:+77707993	Msa0929580:CDS
AATGAACCTCGGCAACGCAA+GGG	0.607154	6_2:-77707667	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCTTTATTATGGTCCTTT+AGG	+	chr6_2:77705297-77705316	Msa0929580:intron	30.0%
ATCAAACATAACGATATCAG+AGG	-	chr6_2:77705464-77705483	None:intergenic	30.0%
TATGTCCTCATCTATTAGAA+TGG	-	chr6_2:77705840-77705859	None:intergenic	30.0%
TAGTCTTTCAAAAAGCAGTT+AGG	-	chr6_2:77705929-77705948	None:intergenic	30.0%
TACTCCAATAATATCCAAAC+TGG	+	chr6_2:77706183-77706202	Msa0929580:CDS	30.0%
ATCAAATCTGCATTTGTAGA+CGG	-	chr6_2:77706659-77706678	None:intergenic	30.0%
ACAAATGCAGATTTGATTAG+AGG	+	chr6_2:77706662-77706681	Msa0929580:intron	30.0%
ACAACATATACTTCAGTTCA+GGG	-	chr6_2:77706756-77706775	None:intergenic	30.0%
AACAACATATACTTCAGTTC+AGG	-	chr6_2:77706757-77706776	None:intergenic	30.0%
ACGAATAACAATCAAATTGC+CGG	-	chr6_2:77706788-77706807	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	77704884	77708253	77704884	ID=Msa0929580;Name=Msa0929580
chr6_2	mRNA	77704884	77708253	77704884	ID=Msa0929580-mRNA-1;Parent=Msa0929580;Name=Msa0929580-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0\|0\|0\|0.5\|0.6\|0.66\|6\|0\|473
chr6_2	exon	77704884	77704956	77704884	ID=Msa0929580-mRNA-1:exon:13140;Parent=Msa0929580-mRNA-1
chr6_2	exon	77705721	77705806	77705721	ID=Msa0929580-mRNA-1:exon:13141;Parent=Msa0929580-mRNA-1
chr6_2	exon	77705911	77706328	77705911	ID=Msa0929580-mRNA-1:exon:13142;Parent=Msa0929580-mRNA-1
chr6_2	exon	77706839	77706918	77706839	ID=Msa0929580-mRNA-1:exon:13143;Parent=Msa0929580-mRNA-1
chr6_2	exon	77707390	77707776	77707390	ID=Msa0929580-mRNA-1:exon:13144;Parent=Msa0929580-mRNA-1
chr6_2	exon	77707876	77708253	77707876	ID=Msa0929580-mRNA-1:exon:13145;Parent=Msa0929580-mRNA-1
chr6_2	CDS	77704884	77704956	77704884	ID=Msa0929580-mRNA-1:cds;Parent=Msa0929580-mRNA-1
chr6_2	CDS	77705721	77705806	77705721	ID=Msa0929580-mRNA-1:cds;Parent=Msa0929580-mRNA-1
chr6_2	CDS	77705911	77706328	77705911	ID=Msa0929580-mRNA-1:cds;Parent=Msa0929580-mRNA-1
chr6_2	CDS	77706839	77706918	77706839	ID=Msa0929580-mRNA-1:cds;Parent=Msa0929580-mRNA-1
chr6_2	CDS	77707390	77707776	77707390	ID=Msa0929580-mRNA-1:cds;Parent=Msa0929580-mRNA-1
chr6_2	CDS	77707876	77708253	77707876	ID=Msa0929580-mRNA-1:cds;Parent=Msa0929580-mRNA-1

Gene Sequence

>Msa0929580

ATGTCTGACTTAGGTGGGTTTGTTTTAACTGGAAACGCTTTGGATGCTGCACAAGGAATGCTGTCATTGGCAGGTGGGGGTAACGACTTTCATCCAACGTATACCCCAAGTGAGATGGCAATGATGAGGACATACTTCCTTGAATTTGACACTATGTCTGGAATGGGTCGGATGCCTAACTGCTTTTTGAAAGACTATGGTGGTTATATTGAAGACTACGTTATGTTGCGAGATCCAATGAACAATGAGATTGAGGTTCGACTGTATCGCAGAGGCGACAAGCTTTATATGAGGAAAGGTTGGTATGGACTGAAGGACTTCTACAACATTGAGCTTGGTGCATGGATAGACTTGACATATGAAAGACCAAATCTTATGACCTTGACGATCAACAACAGATTTTTCGAGGAAATTTTGTATCCTGCTCACACTACTCCAATAATATCCAAACTGGACCGTCGTGATTTTGGGGGGAATTTGGATGATTTTTTTGAGACATTCACTATTGAATTGACTTACTCGGATGTTAACTCAGGATGTTTGGTATTCACTGTGGATTGCTTTATATTTCCGTTGTACTCTGCTAACGACCTCGATCCTGTGCGAAGGGACCTGTCTCGGTTGAGGACTTATCGTCTCGAGCTAGATAGCGACATTTTAAAGGCGAGGTTACCTTCATGTTTTGCAAAAGAAGTAGGCGTGCATATTGATGATCATGTTATGCTTCGTGATCCAAACAACAATATCATTGTTGTTGAAGTTCAGAAAAAGAATGGCAAGGTCTACTTCAGACAAGGCTGGTCTAGGGTGAAGGATATTTATAACATTGTAGATGGTGCATGGGTCTATCTGAAATATGCGGGGCCTAATCTACTACTCATGACCATAAGAAACAAGGCTGGATTGGAGATACCATATCCATTATCCGATATATCCCTTGCGTTGCCGAGGTTCATTACTTCATCAGGGGAGAGCCAGTTAGTGCATTTTTTCCGCACATCTGTCAAGATTTTGACTTCGTCTGATGTCCTCTCGGGCACTTTGACTTTGCCGTGGTATGGCTTCTGCCAGAAATCCTTGCCACAGAAACCTACCACACTTACGATTGTTGGTCCGAACGGCTGTACATGGAGATGTGATCTGACCTTCGCTGTTGCAAATAATATGCCTTATGCTATGATTGGTGGTGATTGGAACCTGATTTGCACATCGCAGAGGTTGGTTGCAGGCCATGCAGTTAAGTTCGCTGTTGTCCATGACAAAGATAACACAGTCCTTGGTTTCCGACATACCCCAATACACTGCACCCATGGATACTATGTGAAACCTATGGTCAGCGGCAGTGGTCGATATGTGTACCAGGTCAATCACTATCTCAAATATTGCAAGGCGAAGAGTATGCTGGAACAGATGTCGGGATGA

Protein sequence

>Msa0929580

MSDLGGFVLTGNALDAAQGMLSLAGGGNDFHPTYTPSEMAMMRTYFLEFDTMSGMGRMPNCFLKDYGGYIEDYVMLRDPMNNEIEVRLYRRGDKLYMRKGWYGLKDFYNIELGAWIDLTYERPNLMTLTINNRFFEEILYPAHTTPIISKLDRRDFGGNLDDFFETFTIELTYSDVNSGCLVFTVDCFIFPLYSANDLDPVRRDLSRLRTYRLELDSDILKARLPSCFAKEVGVHIDDHVMLRDPNNNIIVVEVQKKNGKVYFRQGWSRVKDIYNIVDGAWVYLKYAGPNLLLMTIRNKAGLEIPYPLSDISLALPRFITSSGESQLVHFFRTSVKILTSSDVLSGTLTLPWYGFCQKSLPQKPTTLTIVGPNGCTWRCDLTFAVANNMPYAMIGGDWNLICTSQRLVAGHAVKFAVVHDKDNTVLGFRHTPIHCTHGYYVKPMVSGSGRYVYQVNHYLKYCKAKSMLEQMSG*